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2025 Vol. 31, No. 7 Published: 2025-07-31 1057 Hydroxylsafflower Yellow A Affecting the Proliferation Invasion and Apoptosis of Colorectal Cancer Cells by Regulating Glycolysis through Hippo Pathway ZHOU Chu, ZHOU Yu, AN Ling, et al DOI: 10.3969/j.issn.1006-6233.2025.07.01 Objective: To investigate the effect of hydroxysafflor yellow A(HSYA) on the proliferation invasion and apoptosis of colorectal cancer HT29 cells and its mechanism. Methods: HT29 cells were divided into 5 groups: Con group (HT29 cells), HSYA low dose group (HSYA-L group, HT29+20 μmoL/L HSYA), HSYA medium dose group (HSYA-M group, HT29+ 20 μmoL/L HSYA), HSYA high dose group (HSYA-H group, HT29+20 μmoL/L HSYA), HSYA high dose group (HSYA-H group, HT29+30 μmoL/L HSYA) HT29+40 μmoL/L HSYA) and XMU-MP-1 group (Hippo inhibitor XMU-MP-1, HT29+40 μmoL/L HSYA+3 μmoL/L XMU-MP-1). The effect of HSYA on the proliferative activity of HT29 cells was detected by CCK-8 and the half-inhibitory concentration (IC50) was calculated. The proliferation, migration and invasion ability of HT29 cells were detected by EdU proliferation assay, cell scratch assay and Transwell assay. The apoptosis rate was detected by flow cytometry. Glucose consumption and lactic acid production in HT29 cells were detected by corresponding kits. The expression levels of glycolysis and Hippo pathway related proteins were detected by Western Blot. Results: Compared with Con group, cell viability, proliferation rate, mobility, number of transmembrane cells, glucose consumption, lactic acid production, HXK2 and LDHA in HSYA-L, HSYA-M and HSYA-H groups were significantly decreased (P<0.05). The apoptosis rate, p-MST1/2, p-LATS1/2, YAP and TAZ levels were significantly increased (P<0.05) in a dose-dependent manner. Compared with XMU-MP-1 group, cell viability, proliferation rate, mobility, number of transmembrane cells, glucose consumption, lactic acid production, HXK2 and LDHA in HSYA-H group were significantly decreased (P<0.05), while apoptosis rate, p-MST1/2, p-LATS1/2, YAP and TAZ levels were significantly increased (P<0.05). Conclusion: HSYA can significantly inhibit the proliferation, migration and invasion of HT29 cells and induce cell apoptosis, which may be related to the activation of Hippo pathway and the regulation of glycolysis process. 2025 Vol. 31 (7): 1057-1063 [Abstract] ( 50 ) HTML (1 KB)  PDF (2527 KB)  ( 22 ) 1063 Effect of Gimadione on Malignant Biological Behaviors of Colorectal Cancer Cells by Regulating the RhoA/ROCK Signaling Pathway SHI Hongli, WANG Li, HU Shuai, et al DOI: 10.3969/j.issn.1006-6233.2025.07.02 Objective: To investigate the effect of germacrone on the malignant biological behavior of colorectal cancer (CRC) cells based on the RhoA/ROCK signaling pathway. Methods: CRC SW480 cells were grouped into control group, L-germacrone group, M-germacrone group, H-germacrone group, Y-27632 group, and H-germacrone+LPA group. CCK-8 assay kit, flow cytometry, scratch assay, Transwell method, and Western blot were used to detect the proliferation activity, apoptosis, migration, invasion abilities, and RhoA/ROCK signaling pathway protein expression in SW480 cells. Results: Compared with the control group in terms of OD value, apoptosis rate, scratch healing rate, invasive cell number, RhoA, and ROCK protein expression, the L-gemone group, the M-gemone group, the H- Guimarone group, Y-27632 group cell OD values, scratch healing rate, invasive cell number, RhoA, ROCK protein expression was significantly decreased (P< 0.05) and apoptosis rate was significantly increased (P<0.05); Compared with the H-gemone group, the cell OD value (P<0.001), scratch healing rate (P<0.001), number of invasive cells (P<0.001), RhoA (P<0.001), and ROCK (P<0.001) protein expression were significantly higher (P<0.05) in the H-gimenone+LPA group, and the apoptosis rate (P<0.001) was significantly reduced (P<0.05). Conclusion: The effect of gimarone on the malignant biological behaviour of CRC cells may be achieved by inhibiting the RhoA/ROCK signalling pathway. 2025 Vol. 31 (7): 1063-1068 [Abstract] ( 32 ) HTML (1 KB)  PDF (3177 KB)  ( 9 ) 1068 Exploring the Reversal Effect of Feiyanning Formula on the Resistance of Lung Adenocarcinoma Cells to Osimertinib Based on PI3K/AKT Pathway BAI Yueqin, et al DOI: 10.3969/j.issn.1006-6233.2025.07.03 Objective: To discuss the reversal effect of Feiyanning formula on the resistance of lung adenocarcinoma cells to osimertinib (AZD9291) based on the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) pathway. Methods: Human lung adenocarcinoma cell line H1975 was cultured in vitro, and AZD9291 resistant cell line (H1975/AZD) was established. The effect of different concentrations of Feiyanning formula on the activity of H1975/AZD cells was measured by MTT assay. H1975/AZD cells were grouped into H1975/AZD group, low-dose Feiyanning formula group (10g/L), high-dose Feiyanning formula group (15g/L), high-dose Feiyanning formula+PI3K activator (740Y-P) group (15g/L Feiyanning formula+30 μmoL/L 740Y-P), and cells without established drug resistance were marked as the control group. CCK8 method and clone formation assay were performed to detect the proliferation ability of H1975 cells. Flow cytometry was used to measure apoptosis of H1975 cells. The scratch healing experiment was performed to detect the migration ability of H1975 cells in each group. Transwell experiment was performed to measure the invasion of H1975 cells in each group. QRT-PCR was applied to measure the mRNA expression levels of Bcl-2, Bax, PI3K, and AKT in H1975 cells. Western blot was applied to detect Bcl-2, Bax, and PI3K/AKT pathway related proteins in H1975 cells. Results: Compared with the control group, there was no statistically significant difference in various behavioral indicators of cells in the H1975/AZD group (P>0.05). For the H1975/AZD group, the low and high dose Feiyanning formula groups showed a decrease in cell survival rate, clone formation number, scratch healing rate, number of invasive cells, Bcl-2 mRNA and protein, and an increase in cell apoptosis rate, Bax, PI3K, AKT mRNAs, and Bax, p-PI3K, p-AKT proteins (P<0.05). For the high-dose Feiyanning formula group, the trends of the above indicators in the cells of the high-dose Feiyanning formula+740Y-P group were reversed (P<0.05). Conclusion: Feiyanning formula can reverse the resistance of lung adenocarcinoma cells to osimertinib by inhibiting the activation of PI3K/AKT pathway. 2025 Vol. 31 (7): 1068-1075 [Abstract] ( 20 ) HTML (1 KB)  PDF (2043 KB)  ( 5 ) 1075 miR-34 Regulating the PI3K/Akt Signaling Pathway and Mediating Autophagy of Lens Epithelial Cells to Improve Cataract LI Lixian, LI Na, HU Jungui, et al DOI: 10.3969/j.issn.1006-6233.2025.07.04 Objective: To explore the regulatory effect of microRNA-34 (miR-34) on phosphatidylinositol 3 kinase (PI3K)/protein kinase B(Akt) signaling pathway and its mechanism in mediating autophagy of lens epithelial cells (LECs) and improving cataract in rats. Methods: The cataract rat model was established by subcutaneous injection of D-galactose. The rats were randomly divided into control group (subcutaneous injection of the same amount of normal saline), model group (subcutaneous injection of 200 mg/kg of D-galactose), NC-agomir group (subcutaneous injection of 200 mg/kg of D-galactose + tail vein injection of NC-agomir) and miR-34 agomir group (subcutaneous injection of 200 mg/kg D-galactose + tail vein injection of 5 nmoL miR-34 agomir) and LY294002 groups (PI3K/Akt pathway inhibitor, 0.3 mg/kg LY294002+ tail vein injection of 5 nmoL miR-34 agomir). Each group consisted of 15 rats. After treatment, RT-qPCR was used to detect the expression level of miR-34 in the lens of each group. The opacity of lens of cataract rats was observed and scored under a microscope. The pathological changes of the rat lens were observed by hematoxylin-eosin (HE) staining. The LC3 autophagosome formation in LECs was detected by immunofluorescence. The oxidative stress levels in LECs were detected by corresponding kits. Western Blot analysis was performed to detect the expression levels of autophagy related proteins in LECs and PI3K/AKT signaling pathway. Results: Compared with the control group, the expression level of miR-34 in the model group was significantly decreased (P<0.05), typic al cataract appeared, and the lens turbidity score was significantly increased (P<0.05), LECs arrangement was disordered, swelling was obvious, tissue structure was severely damaged, and a large number of vacuolates appeared in the cortex. Beclin-1, LC3 fluorescence intensity and LC3-Ⅱ/LC3-I levels were significantly increased, while SOD, p62, p-PI3K, p-Akt and p-MTOR levels were significantly decreased (P<0.05). Compared with the model group, the NC-agomir group had no significant difference in cataract degree, lens turbidity score, and lens pathological injury degree. There were no significant differences in fluorescence intensity of ROS, MDA, Beclin-1, LC3, LC3-Ⅱ/LC3-I, SOD, p62, p-PI3K, p-Akt and p-MTOR (P>0.05). Compared with the NC-agomir group, the miR-34 expression level of rats in the agomir group was significantly increased (P<0.05), lens turbidity score was significantly decreased (P<0.05), epithelial cell morphology was significantly improved, and cortical vacuoles were reduced. The fluorescence intensity of ROS, MDA, Beclin-1, LC3 and LC3-Ⅱ/LC3-I levels were significantly decreased (P<0.05), while the levels of SOD, p62, p-PI3K, p-Akt and p-MTOR were significantly increased (P<0.05). Compared with the miR-34 agomir group, the expression level of miR-34 in LY294002 group had no significant difference (P > 0.05), the lens turbidity score was significantly increased (P<0.05), the uniformity and integrity of epithelial cells were decreased, the cells were mildly swollen and there were a few vacuoles. ROS, MDA, Beclin-1, LC3 fluorescence intensity and LC3-Ⅱ/LC3-I levels were significantly decreased (P<0.05), while SOD, p62, p-PI3K, p-Akt and p-MTOR levels were significantly increased (P<0.05). Conclusion: miR-34 is expressed at a low level in cataract rats. Upregulating its expression can alleviate oxidative stress and autophagy in rat lens epithelial cells (LECs), and the mechanism involved may be related to the activation of the PI3K/Akt signaling pathway. 2025 Vol. 31 (7): 1075-1082 [Abstract] ( 18 ) HTML (1 KB)  PDF (1845 KB)  ( 5 ) 1082 The Expression of PNMA 5 in Gastric Cancer Tissues and its Relationship with the Malignant Biological Behaviors of Gastric Cancer Cells HUANG Lihua, ZUO Yi, WEI Zhenping, et al DOI: 10.3969/j.issn.1006-6233.2025.07.05 Objective: To investigate the expression of paraneoplastic antigen Ma family member 5 (PNMA5) in gastric cancer (GC) and its relationship with GC cells' proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT). Methods: Cancerous and paracancerous tissue sections from 42 GC patients were collected, and the expression of PNMA was detected by immunohistochemical staining. The GC cell lines SGC-7901, HGC27, N87,NU1, BGC-823, and the human normal gastric mucosal epithelial cell line GES-1 were cultured, and the expression level PNMA5 was detected by Western blot. Stable overexpression of PNMA5 in N87 cells (PNMA5-OE-N87) and negative control cells (PNMA5-OENC-N87), as well as stable knockdown of PNMA5 in HGC27 cells (shPN5-HGC27) and its negative control cells (shPNMA5-NC-HGC27) were constructed by lentivirus transfection. Subsequently the proliferation activity of each group of cells was detected by CCK-8 assay and colony formation assay, the migration activity of each group of cells was detected by assay, the invasive activity of each group of cells was detected by Transwell assay, and the expression levels of EMT-related proteins Vimentin, E-cherin, and N-cadherin were detected by Western blot. The nude mouse GC xenograft models were constructed to observe the effect of PNMA overexpression or knockdown on tumor growth and EMT level in vivo. Results: Compared with adjacent tissues, PNMA5 was upregulated in cancer tissues (P<0.05). Compared with ES-1 cells, PNMA5 was upregulated in all GC cell lines (all P<0.05). Compared with PNMA5-OENC-N87 cells, PNMA5-OE-N87 cells had significantly increased proliferation, migration, and invasion activities, with elevated Vimentin and N-cherin expression levels and reduced E-cadherin expression level (all P<0.05); compared with shPNMA5-NC-H27 cells, shPNMA5-HGC27 cells had significantly reduced proliferation, migration, and invasion activities, with reduced Vimentin and N-cherin expression levels and elevated E-cadherin expression level (all P<0.05). In vivo experiments showed that overexpression of PN5 promoted GC tumor growth and EMT level, while knockdown of PNMA5 inhibited tumor growth and EMT level(all P<0.05). Conclusion: PNMA5 is upregulated in GC and promotes GC cells proliferation, migration, invasion, and EMT, acting as an oncogene in GC. 2025 Vol. 31 (7): 1082-1087 [Abstract] ( 16 ) HTML (1 KB)  PDF (2212 KB)  ( 5 ) 1087 Effect of LncRNA NORAD on Pyroptosis Proliferation and Invasion of Non-Hodgkin Lymphoma Cells Based on miR-146a/TRAF6 Axis LI Yang, DIAO Yuqiao, et al DOI: 10.3969/j.issn.1006-6233.2025.07.06 Objective: To investigate the effect of lncRNA NORAD on pyroptosis, proliferation and invasion of non-Hodgkin's lymphoma cells based on miR-146a/TRAF6 axis. Methods: NHL cell lines Jurkat, Raji and normal B cells were cultured. The expression levels of lncRNA NORAD, miR-146a and TRAF6 were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Raji cell line was selected for lncRNA NORAD knockdown treatment (NORAD-siNC group, NORAD-si group). Western blot was used to detect the expression levels of NOD-like receptor protein 3 (NLRP3), Cleaved caspase1, Gasdermins-DN terminal domain (N-GSDMD) and TRAF6. qRT-PCR was used to detect the expression level of miR-146a. The levels of interleukin-1β (IL-1β) and IL-18 in cell culture supernatant were detected by kits. EDU assay was used to detect cell proliferation activity, and Transwell assay was used to detect cell invasion activity. Then, the correlation between lncRNA NORAD and miR-146a, and between miR-146a and TRAF6 was verified by dual-luciferase activity detection. Results: Compared with normal B cells, the expression of lncRNA NORAD and TRAF6 was increased in Jurkat and Raji cells, while the expression of miR-146a was decreased. Compared with cells in the NORAD-siNC group, cells in the NORAD-si group had increased expression levels of miR-146a, NLRP3, N-GSDMD and Cleaved caspase1, decreased expression level of TRAF6, weakened cell proliferation and invasion activities, and increased levels of IL-1β and IL-18 in cell culture supernatant. The results of dual-luciferase assay showed that there were target binding relationships between lncRNA NORAD and miR-146a, and between miR-146a and TRAF6. Conclusion: lncRNA NORAD is up-regulated in NHL cells. Knocking down the expression of lncRNA NORAD can induce pyroptosis of NHL cells and inhibit cell proliferation and invasion through the miR-146a/TRAF6 axis. 2025 Vol. 31 (7): 1087-1092 [Abstract] ( 12 ) HTML (1 KB)  PDF (2297 KB)  ( 4 ) 1092 The Effect of Wenyang Tongpi Xiaoji Prescription on the Apoptosis of Liver Cancer Mouse Cells by Regulating the Mitochondrial Apoptosis Pathway YANG Chun, XIANG Caiqiong, QIN Xin, et al DOI: 10.3969/j.issn.1006-6233.2025.07.07 Objective: To investigate whether the Wenyang Tongpi Xiaoji prescription (WYTP) can promote apoptosis of liver cancer mouse cells by regulating the mitochondrial apoptosis pathway. Methods: H22 liver cancer cells were cultured, and a nude mouse model of H22 liver cancer transplantation was established. The nude mice were assigned to a control group, a positive drug group, a low-dose WYTP (WYTP-L) group, a medium-dose WYTP (WYTP-M) group, and a high-dose WYTP (WYTP-H) group. Tumor growth was observed, and tumor inhibition rate was calculated. HE staining was used to observe the morphology of tumor tissues. Mitochondrial membrane potential (MMP) was analyzed by mitochondrial red fluorescent probe. TUNEL staining was performed to detect cell apoptosis in tumor tissues. Western blot was used to detect the protein expression levels of Cleaved-Caspase-9, Caspase-9, Caspase-3, Cleaved-Caspase-3, Cyt C, Bax, Bcl-2, and PCNA. Results: The survival status of nude mice in each group was basically good. Compared with the control group, the tumor cells of nude mice in the WYTP-L group, WYTP-M group, WYTP-H group, and positive drug group showed loose arrangement, varying degrees of nuclear condensation and vacuolization, and cell necrosis, the tumor mass, tumor volume, mitochondrial membrane potential, the protein expression levels of Bcl-2 and PCNA decreased, the tumor inhibition rate, number of apoptotic tumor cells, and expression levels of Cleaved-Caspase-9, Caspase-9, Caspase-3, Cleaved-Caspase-3, Cyt C, and Bax proteins in tumor tissue cells increased (P<0.05). There was no statistically prominent difference in the above indicators between the positive drug group and the WYTP-H group (P>0.05). Conclusion: WYTP can promote apoptosis of liver cancer mouse cells and inhibit cell proliferation by regulating the mitochondrial apoptosis pathway. 2025 Vol. 31 (7): 1092-1097 [Abstract] ( 15 ) HTML (1 KB)  PDF (2604 KB)  ( 5 ) 1097 Effect of Hyperoside on Cartilage Tissue Damage in Knee Osteoarthritis Rats through Hippo/YAP Pathway QIAO Xin, ZHU Nan, WANG Leilei DOI: 10.3969/j.issn.1006-6233.2025.07.08 Objective: To investigate the mechanism of hyperoside (HYP) on cartilage tissue damage in knee osteoarthritis (KOA) rats based on Hippo/YAP pathway. Methods: Based on the improved Hulth method, a KOA rat model was constructed. KOA rats were assigned to the model group, the low-dose HYP group (HYP-L, 25 mg/kg HYP), the high-dose HYP group (HYP-H, 50 mg/kg HYP), and the high-dose HYP+VTFP group (YAP inhibitor) (HYP-H+VTFP, 50 mg/kg HYP, 10 mg/kg VTFP), each with 12 rats. Another 12 rats with exposed joint cavities without any other treatment were set as the sham surgery group (sham group). Lequesne MG was used to evaluate rat behavior. ELISA was used to detect TNF-α, IL-1β, IL-6, MMP-3, and MMP-13 in joint fluid. HE and safranin O-green staining were used to observe the pathological condition of cartilage tissue. TUNEL method was used to detect chondrocyte apoptosis. In addition, Western blot was used to detect TAZ and YAP proteins. Results: For the sham group, the model group showed manifest increases in rat behavioral scores, TNF-α, IL-1β, IL-6, MMP-3, and MMP-13, and chondrocyte apoptosis rate, and obvious decreases in TAZ and YAP (P<0.05), the results of HE staining and safranin O-green staining showed severe cartilage tissue defects, reduced cell numbers, rough surface, and disordered staining distribution. For the model group, the HYP-L and HYP-H groups showed manifest reductions in rat behavioral scores, TNF-α, IL-1β, IL-6, MMP-3, and MMP-13, and chondrocyte apoptosis rate, and obvious increases in TAZ and YAP (P<0.05), the thickness and number of cells in cartilage tissue increased, and surface damage was reduced. VTFP could, to some extent, reduce the improvement effect of HYP on cartilage tissue damage in KOA rats. Conclusion: HYP may alleviate cartilage tissue damage in KOA rats by adjusting Hippo/YAP pathway. 2025 Vol. 31 (7): 1097-1102 [Abstract] ( 8 ) HTML (1 KB)  PDF (1976 KB)  ( 3 ) 1102 Impact of LncRNA MALAT1 on IL-1β-Induced Chondrocyte Injury by Targeting miR-106a-5p/Smad7 BAI Bo, MA Jialiang, LIU Yongquan, et al DOI: 10.3969/j.issn.1006-6233.2025.07.09 Objective: To investigate the impact of long non-coding RNA (LncRNA) metastasis associated lung adenocarcinoma transcript 1 (MALAT1) on interleukin-1β (IL-1β) -induced chondrocyte injury, and to determine whether it is related to microRNA-106a-5p (miR-106a-5p)/mother against decapentaplegic homolog 7 (Smad7). Methods: The human chondrocyte line C20/A4 was used as the research subject, and a chondrocyte injury model was induced by IL-1β. The cells were assigned into IL-1β group, IL-1β+sh-NC group, IL-1β+sh-MALAT1 group, IL-1β+sh-MALAT1+inhibitor NC group, and IL-1β+sh-MALAT1+miR-106a-5p inhibitor group. The control group was C20/A4 without IL-1β induction. QRT-PCR was performed to detect the mRNA expression of LncRNA MALAT1, miR-106a-5p, and Smad7. MTT and flow cytometry were performed to detect cell proliferation and apoptosis. ELISA was performed to detect IL-6, IL-8, and IL-10. Dual luciferase reporter assay was used to validate the targeting relationship between miR-106a-5p with LncRNA MALAT1 and Smad7. In addition, Western blot was used to detect apoptosis related proteins. Results: Compared with the control group, the IL-1β group showed increased LncRNA MALAT1, Smad7 mRNA expression, cell proliferation inhibition rate, apoptosis rate, IL-6, IL-8 levels, and Bax protein expression, and decreased miR-106a-5p expression, IL-10 level, and Bcl-2 protein expression (P<0.05). Compared with the IL-1β group and the IL-1β+sh-NC group, the IL-1β+sh-MALAT1 group had reduction in LncRNA MALAT1, Smad7 mRNA expression, cell proliferation inhibition rate, apoptosis rate, IL-6, IL-8 levels, and Bax protein expression, and elevation in miR-106a-5p expression, IL-10 level, and Bcl-2 protein expression (P<0.05). Compared with the IL-1β+sh-MALAT1 group and the IL-1β+sh-MALAT1+inhibitor NC group, the IL-1β+sh-MALAT1+miR-106a-5p inhibitor group showed no significant difference in LncRNA MALAT1 expression (P>0.05), reduced miR-106a-5p expression, IL-10 level, and Bcl-2 protein expression, and elevated Smad7 mRNA expression, cell proliferation inhibition rate, apoptosis rate, IL-6, IL-8 levels, and Bax protein expression (P<0.05). MALAT1 targeted the regulation of miR-106a-5p/Smad7 axis. Conclusion: Silencing MALAT1 can upregulate miR-106a-5p and inhibit Smad7, thereby promoting cell proliferation, inhibiting cell apoptosis and inflammatory response, and alleviating IL-1β - induced chondrocyte injury. 2025 Vol. 31 (7): 1102-1109 [Abstract] ( 14 ) HTML (1 KB)  PDF (1740 KB)  ( 3 ) 1109 TNFAIP3 Alleviating Lumbar Disc Herniation in Rats by Regulating the Sirt1/FOXO3/Wnt/β-catenin Axis WANG Jing, LIU Dafeng DOI: 10.3969/j.issn.1006-6233.2025.07.010 Objective: To investigate the effects of TNFAIP3 (TNFα-induced protein 3) on lumbar disc herniation (LDH) in rats and explore its potential underlying mechanisms. Methods: A rat model of LDH was established through autologous nucleus pulposus transplantation. Eight healthy rats were used as the sham operation group, undergoing the same procedures as the modeling rats except for the placement of the nucleus pulposus. Five days after modeling, the model rats were randomly divided into four groups: model group, positive control group (ibuprofen, 20mg/kg), AAV empty virus group, and TNFAIP3 overexpression group (150 μL AAV-R-TNFAIP3, 1×1012vg/mL), with eight rats in each group. The positive control group received an intraperitoneal injection of ibuprofen at 20mg/kg. The TNFAIP3 overexpression group received an intraperitoneal injection of AAV-R-TNFAIP3 viral vector, while the AAV empty virus group received an equal volume of AAV empty viral vector, both administered once a week. The sham operation and model groups received daily intraperitoneal injections of an equal volume of saline throughout the study period. After four weeks of continuous treatment, the mechanical paw withdrawal threshold (MPWT) was assessed using the Von Frey filament test to evaluate the analgesic effect. Pathological changes in lumbar tissues were observed by hematoxylin and eosin (HE) staining. Serum levels of proinflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6), were measured by enzyme-linked immunosorbent assay (ELISA). The mRNA and protein expression of TNFAIP3, Sirt1, FOXO3, Wnt3a, and β-catenin in lumbar tissues were detected by qRT-PCR and Western Blot, respectively. Results: Compared with the sham operation group, the model and AAV empty virus groups showed significantly decreased MPWT (P<0.05), increased inflammatory cell infiltration and pathological damage in lumbar tissues (P<0.05), elevated serum levels of TNF-α, IL-1β, and IL-6 (P<0.05), and downregulated mRNA and protein expression of TNFAIP3, Sirt1, and FOXO3 (P<0.05) with upregulated Wnt3a and β-catenin (P<0.05) in lumbar tissues. Compared with the model group, the positive control and TNFAIP3 overexpression groups exhibited significantly increased MPWT (P<0.05), reduced inflammatory cell infiltration and pathological damage in lumbar tissues (P<0.05), and decreased serum levels of TNF-α, IL-1β, and IL-6 (P<0.05). Notably, the TNFAIP3 overexpression group showed significantly upregulated mRNA and protein expression of TNFAIP3, Sirt1, and FOXO3 (P<0.05) and downregulated Wnt3a and β-catenin (P<0.05) in lumbar tissues. Conclusion: TNFAIP3 improves the symptoms and pathological changes of LDH in rats by modulating the Sirt1/FOXO3/Wnt/β-catenin axis. 2025 Vol. 31 (7): 1109-1115 [Abstract] ( 12 ) HTML (1 KB)  PDF (1489 KB)  ( 3 ) 1115 Effect of Triptolide on Inflammatory Response of Acute Gouty Arthritis Rats by Adjusting TXNIP/NLRP3 Pathway ZHAO Xiaoyan, LI Zhipeng, XU Kang DOI: 10.3969/j.issn.1006-6233.2025.07.011 Objective: To investigate the effect of triptolide (TPL) on inflammatory response of acute gouty arthritis (AGA) rats by adjusting TXNIP/NLRP3 pathway. Methods: SD rats were assigned into sham surgery group (Sham), AGA group, AGA+TPL group, AGA+TPL+Ad-NC group, and AGA+TPL+Ad-TXNIP group, each with 12 rats. The degree of ankle joint swelling was measured before modeling and at 4 hours, 8 hours, 24 hours and 48 hours of modeling. Reactive oxygen species (ROS) and superoxide dismutase (SOD) activity detection kits were used to measure ROS and SOD activities. Enzyme-linked immunosorbent assay (ELISA) was used to measure interleukin (IL)-1β and IL-18 in ankle joint tissue. Hematoxylin-eosin (HE) staining was used to observe the ankle joint tissues. Immunohistochemical staining was used to observe TXNIP in ankle joint tissues. Western blot experiments were used to test TXNIP/NLRP3 pathway related proteins. Results: For the Sham group, the AGA group showed clearly increased swelling degree of ankle joint at each time point, ROS, IL-1β, IL-18, TXNIP, NLRP3, and Caspase 1 (P<0.05), and clearly decreased SOD activity (P<0.05) in rats, the synovial tissue was clearly thickened, inflammatory cells were increased, chondrocytes were noticeably swollen, and joint swelling occurred. For the AGA group, the AGA+TPL group showed clearly decreased swelling degree of ankle joint at 8, 24, and 48 hours after modeling, ROS, IL-1β, IL-18, TXNIP, NLRP3, and Caspase 1 (P<0.05), and clearly increased SOD activity (P<0.05) in rats, the thickness of inflammatory cells and synovial tissue decreased, and inflammation and edema were alleviated. For the AGA+TPS+Ad-NC group, the AGA+TPS+Ad-TXNIP group showed clearly increased swelling degree of ankle joint at 8, 24, and 48 hours after modeling, ROS, IL-1β, IL-18, TXNIP, NLRP3, and Caspase 1 (P<0.05), and clearly decreased SOD activity (P<0.05) in rats, the inflammatory cells and synovial tissue thickness increased, but severe inflammation and edema still occurred in the joints. Conclusion: TPL may alleviate ankle joint injury in AGA rats by adjusting key proteins in TXNIP/NLRP3 pathway, reducing inflammatory response and oxidative stress. 2025 Vol. 31 (7): 1115-1120 [Abstract] ( 9 ) HTML (1 KB)  PDF (1604 KB)  ( 3 ) 1121 Effects of miR-93-5p on Renal Mesangial Cell Damage in Lupus Nephritis by Targeting ROCK2 WANG Jing, et al DOI: 10.3969/j.issn.1006-6233.2025.07.012 Objective: To investigate the effects of microRNA-93-5p (miR-93-5p) on renal mesangial cell (RMC) damage in lupus nephritis (LN) mice. Methods: QRT-PCR was used to detect the expression of miR-93-5p and ROCK2 mRNA in the serum of 33 LN patients (LN group) treated in our hospital from July 2023 to May 2024 and 33 pairs of healthy individuals (control group) undergoing physical examination. RMCs (identified by morphology) were isolated and purified from LN mice and assigned into Control group, NC-mimics group (transfected with NC-mimics), miR-93-5p-mimics group (transfected with miR-93-5p-mimics), miR-93-5p-mimics+OE-NC group (transfected with miR-93-5p-mimics+OE-N), and miR-93-5p-mimics+OE-ROCK2 group (transfected with miR-93-5p-mimics+OE-ROCK2). The expression of miR-93-5p and ROCK2 mRNA was detected in the cell lysates of each group. MTT and flow cytometry were used to detect the effects of overexpression of miR-93-5p on RMC proliferation and apoptosis, respectively. ELISA kit was used to detect the expression of TNF-α, IL-6, and IL-1β in in the supernatant of RMC cells. In addition, Western blot was used to detect the expression of PCNA, Bcl-2, Cleaved Caspase-3, and ROCK proteins in the lysate of RMC cells. Results: Compared with control group, LN group showed a decrease in miR-93-5p (t=15.977,P<0.001) and an increase in ROCK2 (t=11.292,P<0.001). Compared with the Control group and the NC-mimics group, the values of A570, the expressions of miR-93-5p, PCNA and Bcl-2 in the miR-93-5p-mimics group were increased. The expressions of apoptosis rate, ROCK2 mRNA and protein, TNF-α, IL-6, IL-1β, and Cleaved Caspase-3 decreased (P<0.05). Compared with miR-93-5p-mimics group and miR-93-5p-mimics+OE-NC group, the miR-93-5p-mimics+OE-ROCK2 group showed a decrease in A570 value, miR-93-5p, PCNA, and Bcl-2, and an increase in apoptosis rate, ROCK2 mRNA and protein, TNF-α, IL-6, IL-1β, and Cleaved Caspase-3 expression (P<0.05). Conclusion: miR-93-5p inhibits RMC damage in LN mice by targeting ROCK2. 2025 Vol. 31 (7): 1121-1127 [Abstract] ( 13 ) HTML (1 KB)  PDF (1686 KB)  ( 3 ) 1127 Construction of a Predictive Model for Recurrence Risk of Childhood IgA Vasculitis and the Correlation Analysis between Chinese Medicine Syndrome Elements and Recurrence ZHANG Boyan, et al DOI: 10.3969/j.issn.1006-6233.2025.07.013 Objective: To explore the risk factors for recurrence of IgA vasculitis in children, establish a recurrence risk prediction line graph model, and investigate the distribution of Chinese medicine syndromes. Methods: A retrospective case-control study was conducted using 200 patients with initial diagnosis of IgAV who were treated at the outpatient and inpatient departments of Beijing Children's Hospital, Capital Medical University from 2018 to 2023. Patients were divided into a recurrence group of 100 cases and a non-recurrence group of 100 cases (no recurrence within one year after initial cure) based on whether they exhibited characteristic manifestations of IgA vasculitis again within one month after symptom resolution. The study used R software (version 4.4.2) to perform univariate and multivariate Logistic regression analysis to screen for independent characteristics associated with IgA vasculitis recurrence, constructing a line graph to predict the recurrence of children. If a variable was observed to have a statistically significant association with IgA vasculitis recurrence in univariate Logistic regression analysis, it would be included in multivariate Logistic regression analysis to further screen for independent characteristics associated with IgA vasculitis recurrence. The rms package was used to construct the line graph and visualize the data. Univariate and multivariate Logistic regression analyses were performed to compare the distribution of Chinese medicine syndromes between the two groups, identifying Chinese medicine syndromes associated with IgA vasculitis recurrence. P<0.05 was considered statistically significant. Results: The duration of the initial illness, the location of rash involvement, NLR, C-reactive protein, D-dimer, and IgE were independently associated with the occurrence of recurrence (P<0.05), based on the results of multi-factor Logistic regression analysis, a risk prediction model for recurrent IgA vasculitis in children was constructed, with an area under the ROC curve (AUC) of 0.868. The decision curve indicates that using a line plot to predict recurrence in children with IgA vasculitis offers high clinical net benefit. The calibration curve results showed that the line plot demonstrates good calibration across different prediction probability ranges, with minimal deviation between predicted values and actual recurrence rates. Single-factor Logistic regression analysis revealed statistically significant differences in four disease factors lung, large intestine, heat, and external wind in both the recurrent and non-recurrent groups (P<0.05). Incorporating these four disease factors into a multi-factor Logistic regression analysis, the large intestine in disease factors and heat in disease factors were independently associated with recurrence (P<0.05). Conclusion: The number of days since initial onset, affected areas of rash, NLR, C-reactive protein, D-dimer, and IgE are independent influencing factors for recurrent IgA vasculitis in children. The line plot model constructed based on these influencing factors has high clinical net benefit and is reliable and accurate in clinical application. "Large intestine" and "heat" in traditional Chinese medicine disease factors are independent risk factors for the recurrence of IgA vasculitis. 2025 Vol. 31 (7): 1127-1133 [Abstract] ( 19 ) HTML (1 KB)  PDF (1747 KB)  ( 7 ) 1134 Effect of Electroacupuncture Combined with Spleen-Invigorating and Qi-Regulating Method on Intestinal Flora Disorder in Sepsis Rats via TLR4/NF-κB Signaling Pathway ZANG Hongmin, CUI Ying, et al DOI: 10.3969/j.issn.1006-6233.2025.07.014 Objective: To explore the improvement effect of electroacupuncture combined with spleen-invigorating and qi-regulating formula on intestinal flora disorder in sepsis rats and its possible molecular mechanism based on the TLR4/NF-κB signaling pathway. Methods: SD rats were divided into sham operation group, model group, electroacupuncture group, spleen and qi formula group, and electroacupuncture + spleen and qi formula group, and the sepsis rat model was constructed by cecal ligation and perforation. HE staining was used to detect pathological damage of colon tissues. ELISA was applied to detect serum levels of TNF-α, IL-6, and IL-1β. 16S rRNA sequencing was performed to analyze intestinal flora. Western blot was used to detect the expression of TLR4, p-p65 NF-κB/p65 NF-κB, p-IκB and other proteins related to the TLR4/NF-κB signaling pathway in colon tissues. Results: Compared with the sham operation group, the model group showed obvious pathological damage in colon tissues, significantly increased serum levels of TNF-α, IL-6, and IL-1β (P<0.05), and decreased alpha diversity indices (observed_species, Chao1, Shannon, Simpson) of intestinal flora (P<0.05). At the phylum level, the relative abundance of Proteobacteria increased, while that of Firmicutes and Bacteroidetes decreased. At the genus level, the relative abundances of Alloprevotella and Bifidobacterium (related to short-chain fatty acid production and anti-inflammation) decreased, while those of Escherichia-Shigella and Klebsiella (related to lipopolysaccharide production) increased. The expression of TLR4, p-p65 NF-κB/p65 NF-κB, and p-IκB proteins in colon tissues significantly increased (P<0.05). Compared with the model group, the electroacupuncture group and spleen-invigorating and qi-regulating formula group showed alleviated pathological damage in colon tissues, significantly decreased serum levels of TNF-α, IL-6, and IL-1β (P<0.05), increased alpha diversity indices of intestinal flora (P<0.05), increased relative abundances of Firmicutes, Bacteroidetes, Alloprevotella, and Bifidobacterium, decreased relative abundances of Proteobacteria, Escherichia_Shigella, and Klebsiella, and significantly decreased expression of TLR4, p-p65 NF-κB/p65 NF-κB, and p-IκB proteins in colon tissues (P<0.05). The effect of electroacupuncture combined with spleen-invigorating and qi-regulating formula was significantly better than that of single electroacupuncture or formula (P<0.05). Conclusion: Electroacupuncture combined with spleen-invigorating and qi-regulating formula may improve intestinal flora disorder and inhibit inflammation by inhibiting the TLR4/NF-κB signaling pathway, thereby reducing intestinal damage in sepsis rats, and the combination of the two therapeutic methods has a synergistic effect. 2025 Vol. 31 (7): 1134-1139 [Abstract] ( 12 ) HTML (1 KB)  PDF (1839 KB)  ( 6 ) 1140 Effects of Geniposide on Gefitinib Resistance in Non-Small Cell Lung Cancer Cells by Regulating Akt/MDM2/p53 Signaling Pathway QIAN Xia, NI Feng, TAN Cheng, et al DOI: 10.3969/j.issn.1006-6233.2025.08.015 Objective: To explore whether geniposide (GEN) can reduce gefitinib resistance in non-small cell lung cancer (NSCLC) cells by regulating the Akt/MDM2/p53 signaling pathway. Methods: Human NSCLC cell lines PC9 sensitive to gefitinib and PC9/GR resistant to gefitinib were cultured in vitro. PC9/GR cells with good growth status were stochastically assigned into Control group, L-GEN, M-GEN, H-GEN groups (5, 10, 20mg/mL GEN), and H-GEN+SC79 group (20mg/mL GEN+8μg/mL Akt activator SC79). CCK8 method was performed to detect cell proliferation. The plate cloning experiment was used to detect the ability of cells to form clones. Flow cytometry was performed to detect cell apoptosis. Transwell experiment was performed to detect cell invasion. Western blot was used to detect MDR1, MMP-2, MMP-9, and Akt/MDM2/p53 signaling pathway proteins. Results: The L-GEN group, M-GEN group, and H-GEN group had lower OD450 values of PC9/GR cells, colony formation rates, cell invasion counts, MDR1, MMP-2, MMP-9, and p-Akt/Akt, p-MDM2/MDM2 protein expression compared to the Control group, while apoptosis rates and p53 protein expression were higher (P<0.05); the H-GEN+SC79 group showed higher OD450 values, colony formation rates, cell invasion counts, MDR1, MMP-2, MMP-9, p-Akt/Akt, p-MDM2/MDM2 protein expression and lower apoptosis rates and p53 protein expression compared to the H-GEN group (P<0.05). Conclusion: GEN primarily weakens the resistance of NSCLC cells to gefitinib by inhibiting the Akt/MDM2/p53 pathway. 2025 Vol. 31 (7): 1140-1144 [Abstract] ( 16 ) HTML (1 KB)  PDF (2191 KB)  ( 5 ) 1145 Correlation of miR-1297 and BCAT1 Expression with Pathological Parameters Epithelial-Mesenchymal Transition and Prognosis in Gastric Cancer Tissues ZHANG Xia, DENG Shuangnian, YANG Xiaojuan DOI: 10.3969/j.issn.1006-6233.2025.07.016 Objective: To investigate the relationship between the expression of microRNA-1297 (miR-1297) and branched-chain amino acid transaminase 1 (BCAT1) in gastric cancer tissues with pathological parameters, epithelial-mesenchymal transition (EMT) and prognosis. Methods: A total of 150 gastric cancer patients who underwent surgical resection from January 2017 to September 2019 (gastric cancer group) and 65 patients undergoing gastrointestinal polyp surgery during the same period (control group) were selected. The expression of miR-1297, BCAT1 mRNA, and EMT markers [vimentin (VIM), snail family transcriptional repressor 1 (SNAI1), E-cadherin (E-cad), N-cadherin (N-cad)] in gastric cancer and gastrointestinal polyp tissues were detected using real-time quantitative polymerase chain reaction. Binding sites between miR-1297 and BCAT1 were predicted through an online database, and the correlation between the expression of miR-1297, BCAT1 mRNA, and EMT markers in gastric cancer tissues was analyzed using Pearson correlation analysis. Patients were grouped into high and low expression groups based on the mean expression levels of miR-1297 and BCAT1 mRNA, and Kaplan-Meier survival curves were plotted for patients with different miR-1297 and BCAT1 mRNA expression levels. Multivariate Cox regression analysis was used to explore the relationship between miR-1297, BCAT1 mRNA expression, and prognosis in gastric cancer patients. Results: Compared with gastrointestinal polyp tissues, miR-1297 and E-cad mRNA expression levels were lower in gastric cancer tissues, while BCAT1 mRNA, VIM mRNA, SNAI1 mRNA, and N-cad mRNA expression levels were higher (P<0.05). A binding site was identified at the 3' untranslated region (4135-4142) of BCAT1 for miR-1297. In gastric cancer tissues, miR-1297 expression was negatively correlated with BCAT1 mRNA, VIM mRNA, SNAI1 mRNA, and N-cad mRNA expression but positively correlated with E-cad mRNA expression (P<0.05). BCAT1 mRNA expression was positively correlated with VIM mRNA, SNAI1 mRNA, and N-cad mRNA but negatively correlated with E-cad mRNA (P<0.05). miR-1297 expression was lower and BCAT1 mRNA expression higher in poorly differentiated, TNM stage III, and lymph node metastatic gastric cancer tissues compared to moderately and well-differentiated, TNM stage I-II, and non-metastatic tissues (P<0.05). The 3-year overall survival rate of 150 gastric cancer patients was 61.33% (92/150). Patients with high miR-1297 expression had higher 3-year overall survival rates than those with low miR-1297 expression, while patients with high BCAT1 mRNA expression had lower survival rates compared to those with low BCAT1 mRNA expression (P<0.05). Poor differentiation, TNM stage III, lymph node metastasis, and BCAT1 mRNA ≥2.38 were independent risk factors for mortality, while miR-1297 ≥1.33 was an independent protective factor (P<0.05). Conclusion: miR-1297 expression is low and BCAT1 mRNA expression is high in gastric cancer tissues, and both are associated with unfavorable pathological characteristics, EMT, and poor prognosis. These factors may serve as novel biomarkers for evaluating EMT and prognosis in gastric cancer patients. 2025 Vol. 31 (7): 1145-1151 [Abstract] ( 10 ) HTML (1 KB)  PDF (1423 KB)  ( 5 ) 1152 Expression and Clinical Significance of miR-214-3p and THBS2 in Cervical Cancer Tissues YAN Qiongqiong, LI Yaping, ZHU Haijun, et al DOI: 10.3969/j.issn.1006-6233.2025.07.017 Objective: To investigate the expression of microRNA-214-3p (miR-214-3p) and thrombospondin 2 (THBS2) in cervical cancer tissues and their relationship with pathological parameters and prognosis. Methods: A total of 84 cervical cancer patients who underwent total hysterectomy or radical hysterectomy in our hospital from January 2019 to March 2021 were enrolled. The expression levels of miR-214-3p and THBS2 mRNA in cervical cancer and adjacent tissues were detected by real-time quantitative PCR. THBS2 protein expression was assessed by immunohistochemistry. The binding sites between miR-214-3p and THBS2 were predicted using the TargetScan database, and Pearson correlation analysis was performed to determine the correlation between miR-214-3p and THBS2 expression in cervical cancer tissues. Based on the median expression levels, cervical cancer tissues were divided into high/low miR-214-3p and THBS2 mRNA expression groups. Kaplan-Meier survival curves were plotted to evaluate progression-free survival (PFS) in cervical cancer patients with high/low miR-214-3p and THBS2 mRNA expression. Multivariate Cox regression analysis was performed to identify factors influencing patient prognosis, using the prognosis of cervical cancer patients as the dependent variable. Results: The expression level of miR-214-3p was significantly lower in cervical cancer tissues (0.99±0.13) than in adjacent tissues (1.55±0.18), while THBS2 mRNA expression was higher in cancer tissues (1.13±0.07) compared to adjacent tissues (0.78±0.12) (t=-22.522 and 23.443, all P<0.001). A binding site was identified at positions 3934-3941 in the 3′ untranslated region of THBS2 for miR-214-3p. A negative correlation was observed between miR-214-3p and THBS2 expression in cervical cancer tissues (r=-0.830, P<0.001). The expression of miR-214-3p and THBS2 mRNA was significantly associated with tumor differentiation, FIGO stage, and lymph node metastasis (all P<0.05). During a 3-year follow-up, the progression-free survival rate was 65.48% (55/84). Patients with high miR-214-3p expression had a higher 3-year PFS rate, while those with high THBS2 mRNA expression had a lower 3-year PFS rate (Log-rank χ2=14.120 and 10.350, all P<0.05). Independent risk factors for death in cervical cancer patients included poor differentiation (HR=4.532, 95% CI: 1.230~16.707), FIGO stage III (HR=6.038, 95% CI: 1.712~21.290), lymph node metastasis (HR=9.004, 95% CI: 2.167~37.422), and THBS2 mRNA ≥1.11 (HR=6.521, 95% CI: 1.474~28.841). High miR-214-3p expression (≥1.02) was an independent protective factor (H=0.141, 95% CI: 0.035~0.568) (all P<0.05). Conclusion: Low expression of miR-214-3p and high expression of THBS2 mRNA in cervical cancer tissues are associated with unfavorable pathological characteristics and poor prognosis. 2025 Vol. 31 (7): 1152-1158 [Abstract] ( 12 ) HTML (1 KB)  PDF (1441 KB)  ( 3 ) 1158 The Correlation between the Levels of Serum miR-146a USF2 and sCD74 and the Severity of the Disease in Patients with Sepsis-Induced Acute Kidney Injury and the Impact on Prognosis ZHANG Meixiang, ZUO Zhigang, LIU Xiujuan DOI: 10.3969/j.issn.1006-6233.2025.07.018 Objective: To investigate the correlation between the levels of serum microRNA-146a (miR-146a), upstream stimulatory factor 2 (USF2), and soluble CD74 (sCD74) and the severity of disease in patients with sepsis-associated acute kidney injury (AKI), as well as their impact on prognosis. Methods: A total of 144 patients with sepsis-induced AKI from January 2022 to January 2024 in Qinhuangdao First Hospital were selected. Based on the 28-day prognosis, patients were categorized into the good prognosis group and the poor prognosis group. The clinical data, serum levels of miR-146a, USF2, and sCD74 were compared between the two groups. The Spearman correlation coefficient was used to analyze the correlation between serum miR-146a, USF2, and sCD74 and the severity of sepsis and AKI. The relationship between serum miR-146a, USF2, and sCD74 and prognosis was analyzed using partial correlation analysis. Receiver operating characteristic (ROC) curves were evaluated for the predictive value of each indicator for prognosis. Results: Severity of sepsis, severity of AKI, APACHE II, and SOFA scores in the poor prognosis group were higher than in the good prognosis group (P<0.05). The levels of serum miR-146a, USF2, and sCD74 in the poor prognosis group were higher than in the good prognosis group (P<0.05). The levels of serum miR-146a, USF2, and sCD74 in patients with sepsis shock were higher than those in patients with sepsis (P<0.05). The levels of serum miR-146a, USF2, and sCD74 in patients with severe AKI were higher than in patients with mild AKI (P<0.05). Serum miR-146a, USF2, and sCD74 were positively correlated with sepsis severity and AKI severity (P<0.05). Partial correlation analysis showed serum miR-146a, USF2, and sCD74 were significantly correlated with prognosis (P<0.05). ROC curve analysis showed that the AUC of serum miR-146a, USF2, and sCD74 for predicting the prognosis of patients with sepsis-induced AKI were 0.776, 0.751, and 0.780, respectively, with Youden indexes of 0.438, 0.406, and 0.479, and sensitivities of 64.58%, 64.58%, and 70.83%, respectively. The specificities were 79.17%, 76.04%, and 77.08%, respectively. The AUC of serum miR-146a, USF2, and sCD74 combined to predict the prognosis of patients with sepsis-induced AKI was 0.922, with a Youden index of 0.771, a sensitivity of 89.58%, and a specificity of 87.50%. This significantly improved the predictive value compared to each indicator alone (Z=3.741, 4.120, 3.192, all P<0.001). Conclusion: The levels of serum miR-146a, USF2, and sCD74 in patients with sepsis and AKI are significantly correlated with the severity of sepsis and AKI, and are independent predictors of prognosis, and the predictive value can be enhanced when combined with other tests. 2025 Vol. 31 (7): 1158-1164 [Abstract] ( 11 ) HTML (1 KB)  PDF (1414 KB)  ( 8 ) 1164 Predictive Value of Hcy HbA1c and UA Levels and the Combined Detection of the Occurrence of Diabetic Retinopathy WANG Chao, DONG Chongzhou, ZHANG Jielou, et al DOI: 10.3969/j.issn.1006-6233.2025.07.019 Objective: To explore the predictive value of Hcy, HbA1c, and UA levels and the combined detection of the occurrence of diabetic retinopathy (DR). Methods: A total of 118 patients with type 2 diabetes mellitus (T2DM) who were diagnosed in the hospital from June 2020 to June 2024 were selected. All patients completed an eye exam within 1-2 weeks of admission. Based on the results of ophthalmic examination, 118 patients were divided into DR group (n=62) and NDR group (n=56). Serum Hcy,HbA1c,UA levels in the two groups were detected, and the differences in clinical indicators between two groups were compared with univariate analysis. The independent correlation between Hcy,HbA1c,UA level and DR occurrence was evaluated with multivariate Logistic regression analysis, and the predictive value of Hcy,HbA1c,UA level on DR was assessed by receiver operating characteristic (ROC) curve. Results: Hcy level, intraocular pressure, HbA1c, and UA in the DR group were higher than those in the NDR group (P<0.05). Multivariate Logistic regression analysis showed that Hcy, HbA1c, and UA were the influential factors of diabetic retinopathy (P<0.05). ROC curve analysis indicated that the combined detection of Hcy, HbA1c, and UA had a higher predictive value for DR, with an area under the curve (AUC) of 0.830 (95%CI: 0.750-0.893), which was higher than single detection (Z/P=2.137/0.032, 4.527/<0.001, 2.879/0.004). The sensitivity was 90.32% and the specificity was 66.07%. Conclusion: The elevated levels of serum Hcy, HbA1c and UA are correlated with the occurrence of DR and have high predictive value. The above indicators can be used as potential early screening indicators for DR, which is helpful for clinical risk stratification and early intervention. 2025 Vol. 31 (7): 1164-1169 [Abstract] ( 14 ) HTML (1 KB)  PDF (1456 KB)  ( 6 ) 1169 Correlation between TyG Index and Osteoporosis in Men over 50 Years of Age and Postmenopausal Women with Type 2 Diabetes HE Jianqiu, ZHANG Rui, LIU Xiaoyan, et al DOI: 10.3969/j.issn.1006-6233.2025.07.020 Objective: To explore the correlation between indicators related to glucose and lipid metabolism and osteoporosis in patients with type 2 diabetes mellitus (T2DM). Methods: A total of 404 T2DM patients, including 181 males over 50 years old and 223 postmenopausal females, were selected. Dual-energy X-ray absorptiometry (DXA) was used to detect bone mineral density (BMD) of the lumbar spine (L1~L4), femoral neck, and total hip. According to the T-score, patients were divided into three subgroups: normal group (185 cases), osteopenia group (131 cases), and osteoporosis group (88 cases). Indicators related to glucose and lipid metabolism were measured, and the TyG index was calculated to compare differences among groups. Results: In male patients, TyG index in the normal group was higher than that in the osteopenia and osteoporosis group (F=3.251, P=0.041); and in female patients, TYG index in the normal group was higher than that in the osteopenia and osteoporosis group (F=5.591, P=0.004), the uric acid in normal group was higher than that in the osteopenia and osteoporosis group (F=17.203, P<0.001). Logistic regression analysis showed that the TyG index was a protective factor for osteoporosis in male T2DM patients over 50 years old (OR=0.405, P=0.049). TyG index (OR=0.834,P<0.001) and uric acid (OR=0.991, P<0.001) were the protective factors for osteoporosis in postmenopausal women with T2DM. Conclusion: TyG index is a protective factor for osteoporosis in male T2DM patients over 50 years old and in postmenopausal women, and uric acid is a protective factor for osteoporosis in postmenopausal women with T2DM. 2025 Vol. 31 (7): 1169-1172 [Abstract] ( 11 ) HTML (1 KB)  PDF (1179 KB)  ( 6 ) 1172 Correlation Study of Platelet Distribution Width and the Outcome of Patients with Acute Cerebral Infarction after Mechanical Thrombectomy ZHANG Xiawei, FENG Bing, HE Wencheng, et al DOI: 10.3969/j.issn.1006-6233.2025.07.021 Objective: To study the correlation between platelet distribution width and the outcome of acute cerebral infarction after mechanical thrombectomy. Methods: The clinical data of 100 patients with acute cerebral infarction who underwent mechanical thrombectomy in Guiping People's Hospital from January 2022 to January 2024 were retrospectively collected. According to the postoperative outcome, they were divided into the good outcome group [55 cases, modified Rankin score (mRS Score) ≤2] and the poor outcome group (45 cases, mRS Score > 2). The baseline data, PDW value and other relevant clinical indicators of the two groups were analyzed. Results: The PDW level of the patients in the poor outcome group was significantly higher than that in the good outcome group (P<0.05). Multivariate logistic regression analysis showed that NIHSS score and platelet distribution width were independent risk factors for postoperative outcome (all P<0.05). Conclusion: The increased platelet distribution width may indicate a higher risk of poor outcome after mechanical thrombectomy for acute cerebral infarction, and PDW can be used as an important reference index to evaluate the postoperative outcome. 2025 Vol. 31 (7): 1172-1176 [Abstract] ( 16 ) HTML (1 KB)  PDF (1197 KB)  ( 5 ) 1176 Predictive Value of Ultrasonographic Monitoring of Renal Blood Flow Grading Renal Resistance Index Combined with Blood Uric Acid /Serum Creatinine Ratio for Early Warning of Acute Kidney Injury in Patients with Severe Intra-abdominal Infection WANG Jianbin, MENG Lingsheng, YANG Wei, et al DOI: 10.3969/j.issn.1006-6233.2025.07.022 Objective: To investigate the predictive value of ultrasonographic monitoring of renal blood flow (RBF) grading, renal resistance index (RRI) combined with blood uric acid (BUA)/serum creatinine (Scr) ratio for early warning of acute kidney injury (AKI) in patients with severe intra-abdominal infection. Methods: A total of 180 patients with severe intra-abdominal infection admitted from June 2021 to June 2023 were enrolled, and divided into AKI group and non-AKI group according to whether AKI occurred within 7 days of admission. Baseline data, RBF grading, RRI, and BUA/Scr ratio were compared between the two groups. The predictive value of RBF grading, RRI, and BUA/Scr ratio for AKI was analyzed. Additionally, 96 patients with severe intra-abdominal infection admitted from July 2023 to July 2024 were selected as an external validation dataset to verify the predictive value of RBF grading, RRI combined with BUA/Scr ratio for AKI. Results: There were statistically significant differences in the sepsis, septic shock, and organ failure assessment (SOFA) scores, as well as the acute physiology and chronic health evaluation II (APACHE II) scores between the two groups (P<0.05). There were significant differences in RBF grading, RRI, and BUA/Scr ratio between the AKI group and the non-AKI group (P<0.05). RBF grading was negatively correlated with the BUA/Scr ratio, while RRI was positively correlated with the BUA/Scr ratio (P<0.05). RBF grading, RRI, and BUA/Scr ratio were significantly correlated with AKI (P<0.05). The AUC of RBF grading and RRI combined with BUA/Scr ratio for predicting AKI was 0.901, which was superior to the predictive value of the three alone (P<0.05). The AUC of AKI in severe abdominal infection patients in the external validation dataset for RBF grading, RRI combined with BUA/Scr ratio early prediction was 0.928. Conclusion: RBF grading, RRI, and BUA/Scr ratio are associated with AKI in patients with severe intra-abdominal infection. The combination of these three indices has a high predictive value for evaluating the occurrence of AKI. 2025 Vol. 31 (7): 1176-1181 [Abstract] ( 11 ) HTML (1 KB)  PDF (1584 KB)  ( 4 ) 1182 Changes in Pregnancy-Associated Plasma Protein-A and Placental Growth Factor Levels in Early Pregnancy and Their Predictive Value for Early-Onset Preeclampsia LU Jiali, WANG Changhong, WAN Yang DOI: 10.3969/j.issn.1006-6233.2025.07.023 Objective: To explore the changes in pregnancy-associated plasma protein A (PAPP-A) and placental growth factor (PLGF) levels in early pregnancy and the predictive value of the above indicators on early-onset preeclampsia. Methods: The clinical data of 138 patients with early-onset preeclampsia in the hospital from January 2019 to January 2024 and the prenatal examination reports of 100 normal pregnant women during the same period were retrospectively analyzed. Patients with early-onset preeclampsia were included in group A. According to the disease severity, patients with mild preeclampsia were enrolled as group A1 (n=79), patients with severe preeclampsia were set as group A2 (n=59). Normal pregnant women were regarded as group B. The changes of PAPP-A and PLGF levels in early pregnancy in group A and group B were observed, and receiver operating characteristic (ROC) curve was plotted to analyze the predictive efficiency of the above indicators on the occurrence of early-onset eclampsia. The levels of PAPP-A and PLGF in group A1 and group A2 at the time of diagnosis were observed, and the correlation between blood pressure at the time of diagnosis and the above two indicators was analyzed by Pearson correlation analysis. Results: Compared with group B, PAPP-A and PLGF in early pregnancy in group A were significantly lower, with statistical differences (P<0.05). ROC curve revealed that the areas under the ROC curves (AUCs) of serum PAPP-A and PLGF levels for early-onset preeclampsia prediction were 0.843 and 0.908, respectively. The systolic blood pressure (SBP) and diastolic blood pressure (DBP) in group A1 were significantly lower than those in group A2 while serum PAPP-A and PLGF were significantly higher, with statistical differences (P<0.05). Pearson correlation analysis indicated that the SBP and DBP in pregnant women were negatively correlated with serum PAPP-A level,and PLGF level. Conclusion: The levels of serum PAPP-A and PLGF in patients with early-onset preeclampsia are decreased in early pregnancy. The two levels show high predictive efficiency on the occurrence of early-onset preeclampsia and are closely related to the blood pressure level of patients. Early detection can provide reference for the prevention of early-onset preeclampsia. 2025 Vol. 31 (7): 1182-1185 [Abstract] ( 13 ) HTML (1 KB)  PDF (1280 KB)  ( 5 ) 1186 Effect of bone Bridge Formation on Mobility in Patients with Ankylosing Spondylitis LIANG Zhiqiang, WANG Minghao, et al DOI: 10.3969/j.issn.1006-6233.2025.07.024 Objective: To investigate the effect of bone bridge formation on the mobility function of patients with ankylosing spondylitis (AS), so as to provide a basis for the development of early intervention measures. Methods: Ninety-two patients with AS who had developed bone bridges in the Department of Rheumatology from March 2020 to October 2024 were selected as the study group, and 94 patients with AS who did not have bone bridges and anterior vertebral bone sclerosis during the same period were selected as the control group. With the help of the measurement of the occipital wall distance and the finger-ground distance, the Schober test and related indexes, the mSASSS score was performed by imaging examination, and the Bath Ankylosing Spondylitis Functional Index (BASFI) score was measured for the patients. The influence of mSASSS on BASFI was analyzed through regression analysis, and the influence of the formation of the bone bridges on the mobility was then analyzed. Results: The occipital-wall distance and finger-ground distance of the patients in the study group were significantly higher than those in the control group, but the results of the Schober test were significantly lower than those in the control group (P<0.001). The mSASSS and BASFI scores of the study group were significantly higher than those of the control group (P<0.05), and the regression analysis showed that there was a significant positive correlation between mSASSS and BASFI (β=0.43, 95%CI 0.27-0.59, p<0.001), indicating that the formation of bone bridges greatly reduces vertebral body mobility in patients with AS, and has a significantly unfavourable effects. Conclusion: Bone bridge formation affects the mobility of AS patients, and early identification and timely intervention are clinically important to optimize the prognosis of patients. 2025 Vol. 31 (7): 1186-1190 [Abstract] ( 11 ) HTML (1 KB)  PDF (1204 KB)  ( 3 ) 1191 Comparative Study of Totally Laparoscopic and Laparoscopic Assisted Distal Gastrectomy on Lymph Node Dissection Surgical Status Inflammatory Factor and Prognosis GAO Zihao, ZHANG Chenggong, DING Fan, et al DOI: 10.3969/j.issn.1006-6233.2025.07.025 Objective: To observe the effects of totally laparoscopic distal gastrectomy (TLDG) and laparoscopic assisted distal gastrectomy (LADG) on lymph node dissection, surgical status, inflammatory factor levels, and prognosis in patients with gastric cancer. Methods: A total of 102 gastric cancer patients from January 2020 to August 2024 were retrospectively selected and divided into TLDG group and LADG group with 51 cases in each group according to different surgical methods. Lymph node dissection, surgical situation, inflammatory factors, and prognosis were compared between the two groups. Results: There were no statistically significant differences in the total number of lymph nodes cleared and the number of positive lymph nodes between the two groups (P>0.05). The length of the main incision and length of postoperative hospital stay in TLDG group were shorter than those in LADG group, and the time for anastomosis was longer than that in LADG group, with statistically significant differences (P<0.05). 24h after surgery, the differences in C-reactive protein (CRP), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) before and after surgery were smaller in TLDG group than in LADG group, with statistically significant differences (P<0.05). The patients were followed up for 4~59 months. There were 7 and 5 deaths in TLDG group and LADG group, respectively, due to tumor related events. The cumulative survival rates were 73.00% and 78.10%, respectively. There was no statistically significant difference in survival curve between the two groups (P>0.05). Conclusion: TLDG and LADG are both effective treatment methods. However, TLDG is better in terms of postoperative recovery, length of main incision, hospital stay, and inflammatory response. 2025 Vol. 31 (7): 1191-1195 [Abstract] ( 12 ) HTML (1 KB)  PDF (1357 KB)  ( 4 ) 1195 Application of Biparametric Magnetic Resonance Imaging Based on PI-RADSv2.1 Combined with fPSA/tPSA in Identifying Benign and Malignant Nodules in Prostate Transitional Zone XIANG Yang, PENG Chuanyong, FAN Ju DOI: 10.3969/j.issn.1006-6233.2025.07.026 Objective: To investigate the application value of biparametric magnetic resonance imaging (bp-MRI) based on the Prostate Imaging-Reporting and Data System version 2.1 (PI-RADSv2.1) combined with free prostate-specific antigen (PSA)/total PSA (fPSA/tPSA) in identifying benign and malignant nodules in prostate transitional zone. Methods: A total of 107 patients with nodules in prostate transitional zone who were admitted to the hospital from March 2021 to October 2024 were reviewed. According to the results of pathological diagnosis, the patients were divided into prostate cancer (PCa) group (37 cases) and prostate hyperplasia group (70 cases). fPSA/tPSA, PI-RADSv2.1 scores, general and clinical data were compared between the two groups. Binary logistic regression analysis was used to screen influencing factors. The value of fPSA/tPSA, PI-RADSv2.1 score, and combination of the two in diagnosing benign and malignant nodules in prostate transitional zone was analyzed using the receiver operating characteristic (ROC) curves. Results: There was a statistically significant difference in the grading of PI-RADSv2.1 scores between the two groups (P<0.05). fPSA and tPSA levels of the PCa group were higher than those of the BPH group (P<0.05). However, the proportions of patients with age, hypertension and diabetes in the two groups were similar (P>0.05). Binary logistic regression analysis showed that fPSA/tPSA and PI-RADSv2.1 score were influencing factors of nodules in prostate transitional zone (P<0.05). When fPSA/tPSA and PI-RADSv2.1 score were used to diagnose nodules in prostate transitional zone separately, the area under the curve (AUC) of PI-RADSv2.1 score was the greatest, which was 0.889. With 3 points as the optimal cutoff value, its diagnostic sensitivity and specificity were 86.49% and 88.57%. Based on binary logistic regression analysis, a joint diagnosis model was constructed as follows: logit(p)=-4.327+1.825 * PI-RADSv2.1 score -15.730 * fPSA/tPSA. Hosmer-Lemeshow test showed χ2=7.585, P=0.475. ROC curve showed that the AUC of joint diagnosis was 0.936. With 0.48 as the optimal cutoff value, the sensitivity and specificity of joint diagnosis were 83.78% and 94.29%. Conclusion: Both fPSA/tPSA and PI-RADSv2.1 score can be used for clinical diagnosis of benign and malignant nodules in prostate transitional zone, and diagnostic value of combination of the two is higher. 2025 Vol. 31 (7): 1195-1200 [Abstract] ( 11 ) HTML (1 KB)  PDF (1329 KB)  ( 3 ) 1200 Correlation Analysis between Helicobacter Pylori Infection and Digestive Tract Diseases HUANG Yafang, JIANG Wanting, LIU Guanxi, et al DOI: 10.3969/j.issn.1006-6233.2025.07.027 Objective: To investigate the correlation between Helicobacter pylori and digestive tract diseases and analyze the risk factors. Methods: The medical records of 2110 and 1247 patients who underwent gastroscopy and colonoscopy in Oriental Hospital of Beijing University of Traditional Chinese Medicine from January 2020 to December 2023 were retrospectively analyzed, and the subjects were divided into the helicobacter pylori positive group and the negative group according to the results of helicobacter pylori detection. Results: The infection rates of Helicobacter pylori in patients with reflux esophagitis, esophageal cancer, chronic atrophic gastritis, gastric ulcer, duodenal ulcer, complex ulcer, gastric polyp and gastric cancer were 73.05%, 81.08%, 77.67%, 86.77%, 82.70%, 91.67%, 68.28% and 77.78%, respectively. There was a significant difference between the infected group and the uninfected group (P<0.05). Among them, the infection rate of Helicobacter pylori in patients with colorectal polyps was 77.80%, and the difference between the infected group and the uninfected group was significant (P<0.05). The infection rates of Helicobacter pylori in patients with chronic atrophic gastritis and colorectal polyps, peptic ulcer and colorectal polyps, gastric polyps and colorectal polyps, gastric cancer and colorectal polyps were 73.23%, 79.61%, 73.37%, and 92.31%, respectively. The infection rate of the infected group was significantly higher than that of the uninfected group (P<0.05). Gender, age, and marital status were independent risk factors for H. pylori infection (P<0.05). Conclusion: Helicobacter pylori infection is closely related to upper and lower digestive tract diseases and complications. In addition, being married and advanced age may be independent risk factors for H. pylori infection. This is essential for the management of Helicobacter pylori infection as well as the treatment and prevention of gastrointestinal disorders. 2025 Vol. 31 (7): 1200-1205 [Abstract] ( 25 ) HTML (1 KB)  PDF (1213 KB)  ( 5 ) 1205 Clinicopathological Significance of SMAD3 and CD44 Expression in Papillary Thyroid Carcinoma and Its Influence on Prognosis LV Dong, TANG Liangcheng, SUN Qing DOI: 10.3969/j.issn.1006-6233.2025.07.028 Objective: To investigate the clinicopathological significance of the expression of signal transduction molecule 3 (SMAD3) and leukocyte differentiation antigen 44 (CD44) in papillary thyroid carcinoma and its influence on prognosis. Methods: The study subjects were 110 patients with papillary thyroid carcinoma received surgical treatment from August 2020 to October 2023. mRNA and protein expression level differences in SMAD3 and CD44 in papillary thyroid carcinoma tissues and peripheral paracancerous tissues were analyzed using real-time quantitative PCR method and immunohistochemistry. According to the presence or absence of cervical lymph node metastasis, the patients were grouped into metastatic group (n=56) and non-metastatic group (n=54). The clinical data and relevant laboratory indicators between the two groups were compared, and the expression levels of SMAD3 and CD44 proteins in lesion tissues were recorded. Univariate and multivariate Logistic analysis was used to analyze the correlation between each index and cervical lymph node metastasis. Results: SMAD3mRNA and CD44mRNA levels were higher in papillary thyroid carcinoma tissues than in paraneoplastic tissues, and the positive expression rate of SMAD3 protein and CD44 protein was higher than that in paraneoplastic tissues (P< 0.05). The mRNA level and protein level of SMAD3 in patients in the metastasis group were higher than those in the non-metastasis group (P<0.05); SMAD3 positive expression was found in 51 cases of 110 patients, accounting for 46.36% of the total; the difference in the positive expression rate of SMAD3 protein in the clinicopathological characteristics of patients with different genders, ages, tumor diameters, and foci conditions was not statistically significant (P＞0.05); the positive expression rate of SMAD3 protein in patients with lymph node metastasis was higher than that in those without lymph node metastasis (P＜0.05); 60 cases of 110 patients had positive expression of CD44 protein, accounting for 54.55% of the total; the difference in the positive expression rate of CD44 protein in the clinicopathological characteristics of patients with different genders, ages, tumor diameters, and foci conditions was not statistically significant (P＞0.05), and the difference in the positive expression rate of CD44 protein in patients with lymph node metastasis was higher than that of those without lymph node metastasis (P<0.05). The levels of NLR, PDW, uric acid, urea nitrogen and creatinine in patients with lymph node metastasis were higher than those in the group without metastasis (P<0.05); the differences in the composition of gender, age, tumor diameter and lesion condition between the two groups were not statistically significant (P>0.05); the results of multifactorial Logistic analysis showed that SMAD3 positive expression, CD44 positive expression and elevated levels of various laboratory indices were the independent risk factors of cervical lymph node metastasis (P<0.05). Conclusion: SMAD3 and CD44 in patients with papillary thyroid cancer showed abnormal expression in cancer tissues, which can be used as an index to improve the early diagnosis rate and provide a reference for the evaluation of lymph node metastasis. 2025 Vol. 31 (7): 1205-1211 [Abstract] ( 12 ) HTML (1 KB)  PDF (1229 KB)  ( 3 ) 1211 Influences of WaveOne Gold Nickel-Titanium File and Hand Stainless Steel K File on Root Canal Preparation and Postoperative Pain in Patients with Apical Periodontitis ZHU Xiujuan, LIU Gang, ZHOU Ya'nan, et al DOI: 10.3969/j.issn.1006-6233.2025.07.029 Objective: To compare the influence of WaveOne Gold nickel-titanium file and hand stainless steel K file on root canal preparation and postoperative pain in patients with apical periodontitis. Methods: Totally 130 patients who received root canal therapy in the hospital from October 2021 to October 2023 were classified into nickel-titanium file group (n=66, WaveOne Gold nickel-titanium file) and K file group (n=64, hand stainless steel K file) according to the instrument selection corresponding to clinical indications. The success rate of one-time root canal preparation and root canal preparation time were compared between groups. The effect of root canal filling and pain status at 7 days after surgery were evaluated. Results: The success rate of one-time root canal preparation in the nickel-titanium file group was higher than that in the K file group, and the root canal preparation time was shorter (P<0.05). The root canal accurate filling rate in the nickel-titanium file group was higher compared with that in the K file group (P<0.05). The visual analogue scale (VAS) score at 7 days after surgery was lower in the nickel-titanium file group than that in the K file group (P<0.05). Conclusion: Compared with hand stainless steel K file, WaveOne Gold nickel-titanium file is more effective in root canal preparation for patients with apical periodontitis, and the latter one can more effectively improve the success rate of one-time root canal preparation, shorten the root canal preparation time, enhance the root canal filling effect, and relieve the postoperative pain. 2025 Vol. 31 (7): 1211-1215 [Abstract] ( 18 ) HTML (1 KB)  PDF (1203 KB)  ( 3 ) 1215 The Predictive Value of Contrast-Enhanced Ultrasound Combined with Serum PRDX6 and LTBP2 in the Sentinel Lymph Node Metastasis of Breast Cancer LI Juan, et al DOI: 10.3969/j.issn.1006-6233.2025.07.030 Objective: To investigate the value of contrast-enhanced ultrasound (CEUS) combined with serum peroxide-reducing protein 6 (PRDX6) and potential transforming growth factor β binding protein 2 (LTBP2) in the prediction of sentinel lymph node metastasis (SLNM) in breast cancer (BC). Methods: A total of 116 BC patients diagnosed by pathology from January 2022 to June 2024 were selected as the study subjects, and all patients underwent CEUS examination before surgery. Enzyme-linked immunosorbent assay (ELISA) was used to measure serum PRDX6 and LTBP2 levels, and sentinel lymph node biopsy (SLNB) and pathological examination were performed during surgery. According to the occurrence of SLNM, 116 patients with BC were divided into a metastatic group (n=46) and a non-metastatic group (n=70). The general data and serum PRDX6 and LTBP2 levels of BC patients in the metastatic group and the non-metastatic group were compared. The receiver operating characteristic (ROC) curve was used to analyze the predictive performance of CEUS, serum PRDX6 and LTBP2 alone and in combination with the detection of SLNM in BC patients. Results: There were no significant differences in age, BMI, menopause, and tumor location between the metastatic group and the non-metastatic group in BC patients (P>0.05), but there were significant differences in tumor diameter, T stage, vascular invasion, and ER status (P<0.05). The sensitivity and specificity of CEUS in the diagnosis of SLNM were 89.13% (41/46) and 88.57% (62/70), respectively, and the accuracy was 88.79% (103/116), respectively, and CEUS was highly consistent with the "gold standard" (Kappa=0.768, P<0.05). The serum levels of PRDX6 and LTBP2 in the metastatic group were significantly higher than those in the non-metastatic group (P<0.05). The results of ROC curves showed that the sensitivity and specificity of PRDX6 in the diagnosis of SLNM were 91.3% and 82.7%, respectively, and the sensitivity and specificity of LTBP2 in the diagnosis of SLNM were 71.7% and 63.1%, respectively. The sensitivity and specificity of CEUS combined with serum PRDX6 and LTBP2 in the diagnosis of SLNM were 91.3% and 82.7%, respectively, and the AUC of the combined diagnosis was significantly higher than that of CEUS, serum PRDX6 and LTBP2 alone (Z=2.548, 2.205, 1.990, P<0.05). Conclusion: CEUS combined with serum PRDX6 and LTBP2 has high predictive value for SLNM in patients with BC, which can provide an important reference for clinical diagnosis and treatment of BC. 2025 Vol. 31 (7): 1215-1219 [Abstract] ( 13 ) HTML (1 KB)  PDF (1452 KB)  ( 3 )

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