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2024 Vol. 30, No. 10 Published: 2024-10-31 1585 Effect of LINC00852 Regulating the miR-671-5p/MYH9 Signaling Axis on Proliferation Invasion and Migration of Non-Small Cell Lung Cancer Cells LI Yaming, ZHANG Jinzhao, LIU Song, et al DOI: 10.3969/j.issn.1006-6233.2024.10.01 Objective: To investigate the effects of long non-coding RNA 00852 (LINC00852) on the proliferation, invasion, and migration of non-small cell lung cancer (NSCLC) cells by regulating the microRNA-671-5p (miR-671-5p)/myosin heavy chain 9 (MYH9) signaling axis. Methods: The expression levels of LINC00852, miR-671-5p, and MYH9 mRNA were detected in NSCLC tissues and adjacent normal tissues using qRT-PCR. A549 cells were randomly divided into several groups: Control group, sh-NC group, sh-LINC00852 group, sh-LINC00852+anti-NC group, and sh-LINC00852+anti-miR-671-5p group. The expression levels of LINC00852, miR-671-5p, and MYH9 mRNA were detected. Cell proliferation, invasion, and migration were assessed by colony formation assay, Transwell assay, and wound healing assay, respectively. Western blot was used to detect the protein expression of PCNA, MYH9, and MMP-9. Dual-luciferase reporter assay was performed to verify the interaction between LINC00852 and miR-671-5p, and between miR-671-5p and MYH9. Results: The expression levels of LINC00852 and MYH9 mRNA were significantly higher in NSCLC tissues compared with adjacent normal tissues, while the expression of miR-671-5p was significantly lower. Knockdown of LINC00852 inhibited the proliferation, invasion, and migration of A549 cells, and downregulated the expression of MYH9, PCNA, and MMP-9, while upregulating the expression of miR-671-5p. Dual-luciferase reporter assay confirmed that LINC00852 could directly target miR-671-5p, and miR-671-5p could directly target MYH9. Conclusion: LINC00852 inhibits the proliferation, invasion, and migration of NSCLC cells by regulating the miR-671-5p/MYH9 signaling axis. 2024 Vol. 30 (10): 1585-1591 [Abstract] ( 79 ) HTML (1 KB)  PDF (2232 KB)  ( 20 ) 1592 Effect of Exosomal Transport of circ_001895 from Type 2 Diabetes Mellitus on Endothelial Progenitor Cells under High Glucose Stress ZHANG Jie, ZHANG Li, WANG Chenfei, et al DOI: 10.3969/j.issn.1006-6233.2024.10.02 Objective: To investigate the effects of exosomal circ_001895 transportation on endothelial progenitor cells under high glucose stress derived from patients with type 2 diabetes (T2DM). Methods: Serum samples were obtained from T2DM patients and divided into serum group and EXO group. The expression levels of circ_001895 in serum and EXO were detected by using RT-qPCR technology. EXOs derived from T2DM patients' serum with significantly elevated circ_001895 expression were named EXO-circ. Endothelial progenitor cells provided by Wuhan Procell were cultured and divided into the following three groups. Control Group: Cultured conventionally for 24 hours without additional treatment. High Glucose (HG) Group: Treated with 30 mmoL/L glucose for 24 hours. HG+EXO-circ Group: Treated with 30 mmoL/L glucose combined with 100μg/mL EXO-circ for 24 hours. The expression of circ_001895 in each group of cells was detected using RT-qPCR technology. Cell proliferation vitality was detected using the CCK-8 method. Cell apoptosis was detected using flow cytometry. The expression of angiogenic markers E-cadherin, N-cadherin, and Vimentin was detected using Western blot technology. Cell migration capacity was detected using the Transwell cell migration assay. Results: Compared to the serum group, the relative expression level of circ_001895 in the EXO group was significantly upregulated (P<0.05).Compared to the control group, the relative expression levels of circ_001895 and E-cadherin and the cell apoptosis rate in the HG group were significantly upregulated (all P<0.05), while cell proliferation vitality, migration capacity, and the relative expression levels of N-cadherin and Vimentin were significantly downregulated (all P<0.05). Compared to the HG group, the relative expression levels of circ_001895 and E-cadherin and the cell apoptosis rate in the HG+EXO-circ group were significantly upregulated (all P<0.05), while cell proliferation vitality, migration capacity, and the relative expression levels of N-cadherin and Vimentin were significantly downregulated (all P<0.05). Conclusion: Transportation of circ_001895 in exosomes from T2DM inhibits proliferation, migration, and vascular generation of endothelial progenitor cells under high glucose stress, while promoting apoptosis. 2024 Vol. 30 (10): 1592-1596 [Abstract] ( 41 ) HTML (1 KB)  PDF (2320 KB)  ( 14 ) 1597 Effect of Cryptotanshinone on Intestinal Injury in Septic Rats by Regulating the Rac1/AKT/NF-κB Signaling Pathway ZHANG Shuliu, LIU Ruirui, CHEN Yugang, et al DOI: 10.3969/j.issn.1006-6233.2024.10.03 Objective: To investigate the effect of cryptotanshinone (CRY) on intestinal injury in septic rats by regulating the Ras related C3 botulinum toxin substrate 1 (Racl)/serine threonine protease (AKT)/nuclear factor kappa B (NF-κB) signaling pathway. Methods: Sepsis rat models were established and grouped into control group, model group, high-dose, medium, and low-dose cryptotanshinone groups (7.0mg·kg-1·d-1CRY-H group, 14.0mg·kg-1·d-1 CRY-M group, 28.0mg·kg-1·d-1 CRY-L group), and high-dose cryptotanshinone+NF-κB pathway activator group (28.0mg·kg-1·d-1 CRY-H+5.0mg·kg-1·d-1 PMA group), with 12 rats in each group. The control group and the model group were treated with the same dose of normal saline once a day for 21 consecutive days. The levels of serum inflammatory factors, oxidative stress and intestinal mucosal barrier indexes in sepsis rats were detected by kit. HE staining was used to observe the pathological morphology of intestinal tissue in rats. The expressions of ZO-1, occludin and pathway proteins in intestinal tissues of rats were detected by immunohistochemistry and Western blotting, respectively. Results: Compared with the control group, the mucosal villi in the model group were disorderly arranged, with some mucosa shedding and necrosis, a large number of inflammatory cells infiltrating, the histopathological score was higher, the serum levels of TNF-α, IL-1β, MDA, endotoxin, and D-lactate increased, the level of SOD decreased, the expression of Rac1, p-AKT/AKT, p-NF-κB/NF-κB was upregulated, while ZO-1 and occludin were downregulated (P<0.05). Compared with the model group, the CRY-L, CRY-M, and CRY-H groups had relatively intact intestinal mucosal villi, inflammatory cell infiltration reduced, and histopathological scores decreased, the serum levels of TNF-α, IL-1β, MDA, endotoxin, and D-lactate decreased, the level of SOD increased, the expression of Rac1, p-AKT/AKT, p-NF-κB/NF-κB was downregulated, while ZO-1 and occludin were upregulated (P<0.05). Compared with the CRY-H group, the CRY-H+PMA group had more inflammatory cell infiltration in intestinal mucosal cells, the histopathological scores increased, the serum levels of TNF-α, IL-1β, MDA, endotoxin, and D-lactate increased, the level of SOD decreased, the expression of Rac1, p-AKT/AKT, p-NF-κB/NF-κB was upregulated, while ZO-1 and occludin were downregulated (P<0.05). Conclusion: Cryptotanshinone inhibits the Rac1/AKT/NF-κB signaling pathway and improves intestinal injury in septic rats. 2024 Vol. 30 (10): 1597-1602 [Abstract] ( 27 ) HTML (1 KB)  PDF (2521 KB)  ( 7 ) 1603 Effects of Dexmedetomidine on Inflammatory Injury in Burned Rats by Regulating the cGAS-STING Signaling Pathway WU Yi, YAN Yuqiang, SU Rui DOI: 10.3969/j.issn.1006-6233.2024.10.04 Objective: To investigate the effects of dexmedetomidine (DEX) on inflammatory injury in burned rats by regulating the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signaling pathway. Methods: Rats were randomly divided into six groups: control group (Control), model group (Model), low dose, medium and high dose DEX groups (DEX-L, DEX-M, DEX-H), and DEX-H + cGAS-STING pathway activator group (DEX-H+DMXAA), with 12 rats in each group. Except for the Control group, all rats were subjected to deep second-degree burns using a burn injury device. The DEX-L, DEX-M, and DEX-H groups received 1, 3, and 5 μg·kg-1·h-1 DEX for 4 hours, respectively. The DEX-H+DMXAA group was administered 25 mg/kg DMXAA and 5 μg·kg-1·h-1 DEX for 4 hours. The Control and Model groups received equivalent amounts of 0.9% saline for 4 hours daily for two weeks. After the intervention, wound healing rates were calculated, serum levels of tumor necrosis factor (TNF-α) and interleukin-1β (IL-1β) were detected by ELISA, and histopathological injury scores were assessed by HE staining. Apoptosis in wound tissues was evaluated by TUNEL staining. The expression of cGAS and STING mRNA in wound tissues was measured by qRT-PCR, while apoptosis-related proteins (Bax, Bcl-2) and cGAS-STING pathway proteins were detected by Western blot. Results: Compared with the Control group, the Model group showed significantly higher levels of TNF-α, IL-1β, wound tissue apoptosis rate, cGAS, STING mRNA, and Bax, cGAS, STING, IRF3 protein expression, while Bcl-2 expression decreased (P<0.05). Compared with the Model group, the DEX-L, DEX-M, and DEX-H groups had significantly increased wound healing rates, higher histopathological scores, elevated Bcl-2 expression, and reduced TNF-α, IL-1β levels, wound tissue apoptosis rate, cGAS, STING mRNA, and Bax, cGAS, STING, and IRF3 protein expression, with statistically significant differences between the dosage groups (P<0.05). In the DEX-H+DMXAA group, wound healing rates, histopathological scores, and Bcl-2 expression were lower, while TNF-α, IL-1β levels, wound tissue apoptosis rate, cGAS, STING mRNA, and Bax, cGAS, STING, and IRF3 protein expression were higher compared with the DEX-H group (P<0.05). Conclusion: DEX has anti-inflammatory effects, reduces cell apoptosis, and promotes wound healing in burned rats, and its mechanism may be related to inhibiting the activation of the cGAS-STING signaling pathway. 2024 Vol. 30 (10): 1603-1608 [Abstract] ( 23 ) HTML (1 KB)  PDF (2242 KB)  ( 8 ) 1609 The Effect of Paeoniflorin on Necroptosis in Chronic Colitis Rats by Regulating the RIP1/RIP3/MLKL Signaling Pathway MU Zhongyan, ZHANG Jianping, et al DOI: 10.3969/j.issn.1006-6233.2024.10.05 Objective: To explore the effect of paeoniflorin (PAE) on necroptosis in chronic colitis rats by regulating the receptor-interacting protein 1 (RIP1)/receptor-interacting protein 3 (RIP3)/mixed lineage kinase domain-like protein (MLKL) signaling pathway. Methods: Healthy SD rats were randomly divided into six groups, with six rats per group: Control group, Model group, and three treatment groups with low, medium, and high doses of PAE administered via gavage for 7 days (1.25g·kg-1·d-1 PAE-L group, 2.5g·kg-1·d-1 PAE-M group, 5g·kg-1·d-1 PAE-H group), and a combination group with high-dose PAE gavage for 7 days plus tail vein injection of RIP1 activator for 7 days (5g·kg-1·d-1 PAE-H + 1.5mg·kg-1·d-1 Z-VAD-fmk group). A chronic colitis model was established, and after successful modeling, blood was collected from the rats via the orbital vein. Colonic mucosa tissues were collected for HE staining to observe the morphology of colon tissues; colonic mucosal damage index (CMDI) scores were analyzed; TUNEL assay was used to detect apoptosis in colon tissues; immunohistochemistry was used to detect the expression of matrix metalloproteinases (MMP-1, MMP-2) in colon tissues; ELISA was used to detect serum levels of inflammatory cytokines (IL-6, TNF-α); and western blotting was used to detect the expression of RIP1/RIP3/MLKL signaling pathway proteins in colon tissues. Results: Compared with the Control group, the Model group exhibited colonic mucosal erosion, edema, and inflammatory cell infiltration, with increased CMDI scores, apoptosis rate, IL-6, TNF-α levels, and upregulated expression of MMP-1, MMP-2, p-RIP1/RIP1, p-RIP3/RIP3, and p-MLKL/MLKL proteins (P<0.05). Compared with the Model group, the PAE-L, PAE-M, and PAE-H groups showed a gradual reduction in inflammatory cell infiltration in colonic mucosa tissues, decreased CMDI scores, apoptosis rate, IL-6, TNF-α levels, and downregulated expression of MMP-1, MMP-2, p-RIP1/RIP1, p-RIP3/RIP3, and p-MLKL/MLKL proteins (P<0.05). Compared with the PAE-H group, the PAE-H + Z-VAD-fmk group showed exacerbated inflammation in colonic mucosa tissues, with increased CMDI scores, apoptosis rate, IL-6, TNF-α levels, and upregulated expression of MMP-1, MMP-2, p-RIP1/RIP1, p-RIP3/RIP3, and p-MLKL/MLKL proteins (P<0.05). Conclusion: Paeoniflorin can alleviate necroptosis in chronic colitis rats by inhibiting the RIP1/RIP3/MLKL signaling pathway. 2024 Vol. 30 (10): 1609-1614 [Abstract] ( 30 ) HTML (1 KB)  PDF (2775 KB)  ( 6 ) 1615 Effect of Shenling Baizhu Powder on Diarrhea Regulating the Interation Intestinal Flora and Epithedial Cell Junction in Diarrhea Young Rats ZHUGE Yuanli, et al DOI: 10.3969/j.issn.1006-6233.2024.10.06 Objective: To investigate the influence of Shenling Baizhu powder (SLP) on improving diarrhea model and regulating the interaction between intestinal flora and epithelial cell in young rats. Methods: The diarrhea model of young mice was established by gavage of rhubarb water decoction. SLP was administrated by gavage for 2 weeks at the dose of 20.0, 10.0 and 5.0 g herbs / kg. The changes of body weight were measured every week during the experiment; the levels of D-lactic acid and endotoxin in plasma were measured by kit; the morphology and mucus state of colon tissue were detected by PAS staining; the mRNA and protein expression of tight junction protein (occludin, ZO-1 and claudin) were detected by real-time PCR and Western blot respectively; The intestinal flora was analyzed with selective medium. Results: SLP could improve the diarrhea symptoms and significantly inhibit the weight loss. High and medium dose of SLP significantly decreased serum D-lactic acid and endotoxin. The results of PAS staining showed that each dose group of SLP could improve the villi injury of intestinal tissue of model rats, and recover the content of goblet cells and mucus. The expression of ZO-1 and occludin were significantly up-regulated in the high-dose SLP group, and the expression of claudin was also up-regulated. High and medium dose groups of SLP could significantly reduce the number of Enterobacteriaceae and Enterococcus in cecal contents, and recover the number of Lactobacillus and Bifidobacterium. Conclusion: SLP can improve the dysbiosis, increase the expression of tight junction protein, restore the intestinal epithelial cell barrier, and then improve the intestinal symptoms of diarrhea model rats. 2024 Vol. 30 (10): 1615-1620 [Abstract] ( 22 ) HTML (1 KB)  PDF (1577 KB)  ( 8 ) 1621 Effect of Curcumin on Lung Injury in Mice with ARDS via Regulation of the MAPK/NF-κB Signaling Pathway HU Pan, ZHANG Zhuo DOI: 10.3969/j.issn.1006-6233.2024.10.07 Objective: To investigate the protective effects of curcumin on acute respiratory distress syndrome (ARDS) in mice by regulating the MAPK/NF-κB signaling pathway. Methods: BALB/C mice were randomly divided into five groups: control group, model group, curcumin group (200mg/kg), anisomycin group (MAPK activator, 20mg/kg), and curcumin (200mg/kg) + anisomycin (20mg/kg) group, with 12 mice per group. ARDS models were established in the model and drug intervention groups via intratracheal instillation of lipopolysaccharide (LPS), while the control group received an equal volume of normal saline. After 3 days of intervention, the following were measured: lung function indices-forced vital capacity (FVC), peak expiratory flow (PEF), and oxygenation index (OI); inflammatory cell count in bronchoalveolar lavage fluid (BALF); histopathological changes and lung injury scores in lung tissues via HE staining; levels of TNF-α, IL-1β, IL-10, and IL-13 in BALF and serum using ELISA; and MAPK/NF-κB pathway protein expression in lung tissues via Western blotting. Results: Compared with the control group, the model group showed significantly increased inflammatory cell count, lung injury score, TNF-α and IL-1β levels, and p-p38 MAPK/p38 MAPK and p-NF-κB p65/NF-κB p65 ratios (P<0.05), while FVC, PEF, OI, IL-10, and IL-13 levels were significantly decreased (P<0.05). Compared with the model group, the curcumin group had reduced inflammatory cell count, lung injury score, TNF-α and IL-1β levels, and p-p38 MAPK/p38 MAPK and p-NF-κB p65/NF-κB p65 ratios (P<0.05), along with increased FVC, PEF, OI, IL-10, and IL-13 levels (P<0.05). The anisomycin group, compared to the model group, exhibited further increases in inflammatory markers and reductions in lung function indices. Co-administration of curcumin and anisomycin negated the protective effects observed in the curcumin group alone. Conclusion: Curcumin inhibits the activation of the MAPK/NF-κB signaling pathway, reducing inflammation, mitigating lung tissue damage, and improving lung function in ARDS mice, thereby exerting a significant protective effect on the lungs. 2024 Vol. 30 (10): 1621-1626 [Abstract] ( 21 ) HTML (1 KB)  PDF (1722 KB)  ( 4 ) 1627 Exogenous Nerve Growth Factor Activates the Wnt3α/β-Catenin Signaling Pathway to Promote Fracture Healing ZHAO Yanzhe, LIU Jian, SHI Xiaoyun DOI: 10.3969/j.issn.1006-6233.2024.10.08 Objective: To analyze the effect of exogenous nerve growth factor on the activation of Wnt3α/β-catenin signaling pathway in promoting fracture healing. Methods: Select 40 SPF grade SD rats, 10 rats as the control group, and 30 rats to construct a tibial fracture model. A total of 27 rats were successfully modeled and divided into a model group of 9 rats, a low-dose group of 9 rats, and a high-dose group of 9 rats. Observe the pathological histology, bone calcium and phosphorus levels, hemorheological indicators, BMD, callus diameter, angiogenesis related factors, inflammatory factor levels, mRNA expression levels of key genes Wn3a, β -catenin, and GSK-3β in the Wnt3 α/β-catenin pathway, and expression levels of key proteins Wnt3a, β-catenin, and GSK-3β in the Wnt3 α/β-catenin pathway in each group of rats. Results: Compared with the control group, the other three groups showed a decrease in bone calcium, bone phosphorus, BMD, angiogenesis related factors, Wnt3α, β-catenin related mRNA expression, Wnt3α, β-catenin expression, and an increase in whole blood viscosity, plasma viscosity, inflammatory factor levels, GSK-3β related mRNA expression, and GSK-3β expression. Compared with the model group, the low and high dose groups showed an increase in bone calcium, bone phosphorus, BMD, callus diameter, angiogenesis related factors, Wnt3 α, β - catenin related mRNA expression, Wnt3α, β-catenin expression, and a decrease in whole blood viscosity, plasma viscosity, inflammatory factor levels, GSK-3 β related mRNA expression, and GSK-3β expression; And the high-dose group showed the highest levels of bone calcium, bone phosphorus, BMD, callus diameter, angiogenesis related factors, Wnt3α, β-catenin related mRNA expression, Wnt3α, β-catenin expression, and the lowest levels of whole blood viscosity, plasma viscosity, inflammatory factor levels, GSK-3β - related mRNA expression, and GSK-3β expression (P<0.05). Conclusion: The intervention of exogenous nerve growth factor on tibial bone can promote the healing of fracture in rats, the mechanism of which may be related to the activation of Wnt3α/β-catenin signaling pathway, and has a good effect. 2024 Vol. 30 (10): 1627-1633 [Abstract] ( 16 ) HTML (1 KB)  PDF (2174 KB)  ( 4 ) 1634 Effect of Myricetin on Intervertebral Disc Degeneration in Rats with Lumbar Disc Herniation by Regulating Wnt/β-catenin Signaling Pathway LI Na, ZHANG Peng, ZHANG Nan, et al DOI: 10.3969/j.issn.1006-6233.2024.10.09 Objective: To investigate the effect of myricetin (MYR) on intervertebral disc degeneration (IVDD) in rats with lumbar disc herniation (LDH) by regulating the Wnt/β-catenin signaling pathway. Methods: Rats were randomly divided into four groups: Sham group, LDH group, MYR group, and MYR+LiCl group (Wnt/β-catenin signaling pathway activator), with 12 rats in each group. Except for the Sham group, an LDH rat model was replicated using autologous nucleus pulposus transplantation. After successful model establishment, drug intervention was performed once daily for 28 days. Von Frey hair filaments and radiant heat pain tests were used to analyze the pain sensitization behavior of rats. ELISA was used to detect the levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in serum. HE staining was used to observe the pathological changes of intervertebral disc tissue. TUNEL staining was used to determine the apoptosis of nucleus pulposus cells. Immunohistochemistry was used to detect the expression of matrix metalloproteinase 13 (MMP13) in rat intervertebral disc tissue. Western blot was used to detect the protein expression of the Wnt/β-catenin signaling pathway. Results: Compared with the Sham group, rats in the LDH group showed shrinkage and irregular arrangement of nucleus pulposus cells, reduced cell number, and annulus fibrosus rupture. MWT and TWL were decreased, while TNF-α, IL-1β levels, disc tissue pathological score, nucleus pulposus cell apoptosis rate, MMP13, Wnt3a and β-catenin expression were increased (P<0.05). Compared with the LDH group, MYR group showed significant improvement in nucleus pulposus cell morphology and annulus fibrosus structure, increased MWT and TWL, and decreased TNF-α, IL-1β levels, disc tissue pathological score, nucleus pulposus cell apoptosis rate, MMP13, Wnt3a, and β-catenin expression (P<0.05). Wnt/β-catenin signaling pathway activator LiCl increased serum levels of inflammatory factors, promoted nucleus pulposus cell apoptosis, aggravated disc tissue pathological damage, and weakened the delaying effect of MYR on IVDD in LDH rats (P<0.05). Conclusion: Myricetin may improve intervertebral disc degeneration in LDH rats by inhibiting the activation of the Wnt/β-catenin signaling pathway, reducing inflammation, and decreasing nucleus pulposus cell apoptosis. 2024 Vol. 30 (10): 1634-1638 [Abstract] ( 15 ) HTML (1 KB)  PDF (1605 KB)  ( 4 ) 1639 Effect of vacuum Rotary Excision of Breast Nodules in Patients with Breast Nodules with 5-HT MDA and TNF-α by Haurologie WANG Jing, ZHOU Yudong, GUO Pingli DOI: 10.3969/j.issn.1006-6233.2024.10.010 Objective: To investigate the effects of Haurologic breast nodule vacuum rotary excision on neurotransmitters 5-hydroxytryptamine (5-HT), malondialdehyde (MDA) and tumor necrosis factor- α (TNF-α) in patients with breast nodules. Methods: A retrospective analysis was performed on 148 patients with breast nodules admitted to our hospital from April 2020 to May 2023. 46 cases were excluded by inclusion and exclusion criteria, and 102 cases were finally included. According to different surgical methods, they were divided into control group ( n=50, traditional small incision treatment ) and experimental group ( n=52, Holloway vacuum rotary resection of breast nodules ). The surgical observation indexes, the changes of 5-HT, MDA and TNF-α levels before and after treatment, the good rate of postoperative recovery, the incidence of complications, and the recurrence rate of nodules were compared between the two groups. Results: The total operation time of the experimental group was shorter than that of the control group, the incision length was shorter, the amount of blood loss and the total postoperative hospitalization days were less than that of the control group (P<0.05). The levels of 5-HT, MDA and TNF-α increased from before treatment to 3 days after treatment, and the levels of 5-HT, MDA, and TNF-α decreased from 3 days after treatment to 7 days after treatment (P<0.05). The levels of 5-HT, MDA and TNF-α in the experimental group were lower than those in the control group at 1 day, 3 days, and 7 days after treatment (P<0.05). The good recovery rates of control group and experimental group were 78.00% and 92.31%, respectively, and the rate of good recovery was higher in the experimental group (P<0.05). When comparing the recurrence rate of nodules in the two groups, the difference was not statistically significant (P>0.05), and the total complication rate of the experimental group (5.76%) was lower than that of the control group (20.00%) (P<0.05). Conclusion: Halotiere vacuum rotation of breast nodules has relatively little effect on the levels of 5-HT, MDA and TNF-α in patients with breast nodules, and the incidence of complications is low, which has a positive role in promoting the early postoperative recovery of patients. 2024 Vol. 30 (10): 1639-1643 [Abstract] ( 16 ) HTML (1 KB)  PDF (1195 KB)  ( 6 ) 1644 Diagnostic Value of Multi-Slice Spiral CT Combined with Serum ChE α-HBDH and PGRN for Pulmonary Tuberculosis Combined with Lung Cancer ZHONG Linfeng, YUAN Fei, CHEN Xing, et al DOI: 10.3969/j.issn.1006-6233.2024.10.011 Objective: To explore the diagnostic value of multi-slice spiral CT combined with serum cholinesterase (ChE), α-hydroxybutyrate dehydrogenase (α-HBDH), and progranulin (PGRN) in the diagnosis of pulmonary tuberculosis combined with lung cancer. Methods: From June 2021 to June 2023, 84 patients with pulmonary tuberculosis accepted by our hospital were collected. Among them, 36 patients diagnosed with pulmonary tuberculosis combined with lung cancer through pathological examination were made the test group, and 48 patients with simple pulmonary tuberculosis were made the control group. All patients underwent multi-slice spiral CT examination. Enzyme-linked immunosorbent assay (ELISA) was applied to detect the expression levels of ChE, α-HBDH, and PGRN in serum. ROC curve was applied to analyze the diagnostic value of multi-slice spiral CT combined with serum ChE, α-HBDH, and PGRN for pulmonary tuberculosis combined with lung cancer. Results: The proportions of masses, pleural indentation sign, lobulation sign, pleural effusion, spiculation, and nodular shadow sign in the test group were higher than those in the control group (P<0.05). Compared with the control group, the serum ChE level in the test group was reduced, while α-HBDH and PGRN levels were increased (P<0.05). Compared with individual diagnosis, the combination of multi-slice spiral CT and serum ChE, α-HBDH, and PGRN had a higher AUC in the diagnosis of pulmonary tuberculosis combined with lung cancer (z=3.497, P=0.001; z=4.310, P<0.001; z=3.328, P=0.001; z=4.518, P<0.001). Conclusion: Serum ChE expression is low, and the α-HBDH and PGRN expression is high in patients with pulmonary tuberculosis combined with lung cancer. Multi-slice spiral CT combined with serum ChE, α-HBDH, and PGRN has a high diagnostic value for pulmonary tuberculosis combined with lung cancer. 2024 Vol. 30 (10): 1644-1648 [Abstract] ( 15 ) HTML (1 KB)  PDF (1305 KB)  ( 7 ) 1649 Value of Quantitative Echocardiography in Analyzing Cardiac Structure and Function in Patients with Renal Failure Undergoing Maintenance Dialysis WEI Yanfang, HU Min, ZHANG Yunhui DOI: 10.3969/j.issn.1006-6233.2024.10.012 Objective: To analyze the value of quantitative echocardiography in assessing cardiac structure and function in patients with renal failure undergoing maintenance dialysis. Methods: A total of 122 renal failure patients receiving maintenance dialysis at our hospital from November 2021 to March 2024 were selected as the study group, and 143 renal failure patients who had not undergone maintenance dialysis during the same period were selected as the control group. All subjects underwent quantitative echocardiography to obtain cardiac structure and function parameters, and these parameters were compared between the two groups. Results: The study group had higher aortic root diameter (AAo), left ventricular posterior wall thickness (LVPW), and interventricular septal thickness (IVS) compared to the control group (P<0.05). There were no statistically significant differences in aortic root diameter (AARd), left atrial anteroposterior diameter (LA), left ventricular end-diastolic diameter (LVDd), or left ventricular end-systolic diameter (LVDs) between the two groups (P>0.05). The study group had higher values for right ventricular outflow tract velocity time integral (VTI), early diastolic pulmonary regurgitation velocity, late diastolic pulmonary regurgitation velocity, right ventricular ejection time (ET), right ventricular acceleration time (AT), tricuspid annular plane systolic excursion (TAPSE), fractional shortening (FS), left ventricular ejection fraction (LVEF), aortic velocity, pulmonary artery velocity, E peak, and A peak compared to the control group (P<0.05). There was no significant difference between the two groups in terms of maximum tricuspid regurgitation velocity (TRvmax) or left ventricular end-diastolic volume (LVEDV) (P>0.05). The E/A ratio, left ventricular end-systolic volume (LVESV), and pulmonary vascular resistance (PVR) were lower in the study group than in the control group (P<0.05). Conclusion: Quantitative echocardiography can provide comprehensive cardiac structure and function parameters, enabling accurate assessment of cardiac function and structure in patients with renal failure undergoing maintenance dialysis. 2024 Vol. 30 (10): 1649-1652 [Abstract] ( 23 ) HTML (1 KB)  PDF (1174 KB)  ( 15 ) 1653 Analysis of Postoperative Fungal Infections Resistance Characteristics and Influencing Factors in Severe Trauma Patients WU Yujiao, ZHANG Yu, LIU Peipei, et al DOI: 10.3969/j.issn.1006-6233.2024.10.013 Objective: To investigate the incidence of secondary fungal infections following surgery for severe trauma, their drug resistance characteristics, and influencing factors. Methods: A retrospective analysis was conducted on 516 severe trauma patients admitted to the Emergency Department of the First Affiliated Hospital of Air Force Medical University between April 2017 and March 2024. Patients were divided into an infection group (n=145) and a non-infection group (n=371) based on the presence of postoperative fungal infections. The fungal species distribution and drug resistance in the infection group were analyzed, and clinical data were collected for both groups. Logistic regression analysis was performed to identify risk factors, and ROC curves were plotted using the probabilities from the regression equation. Results: A total of 163 fungal strains were isolated from 145 patients in the infection group, with the highest proportion being Candida albicans (84 strains, 51.53%). The main infection sites were the respiratory tract, urinary tract, and surgical sites. Among the 163 strains tested for drug sensitivity, the highest resistance was to itraconazole (26 strains), while the least resistance was observed for flucytosine and amphotericin B (1 strain each). Univariate analysis revealed that factors such as Injury Severity Score (ISS), Sequential Organ Failure Assessment (SOFA) score at admission, duration of invasive ventilation, use and duration of urinary catheters, use of central venous catheters, continuous renal replacement therapy (CRRT), use of corticosteroids, use of antibiotics for ≥7 days, blood glucose levels, blood transfusions, ICU stay duration, and shock at admission were associated with postoperative fungal infections in severe trauma patients (P<0.05). Multivariate logistic regression showed that ISS score, SOFA score, duration of invasive mechanical ventilation, urinary catheter use and its duration, use of central venous catheters, use of corticosteroids, blood transfusion, and ICU stay duration were significant factors influencing postoperative fungal infections (P<0.05). ROC analysis indicated that the AUCs for ISS score, SOFA score, duration of invasive ventilation, urinary catheter use, urinary catheter use for ≥5 days, central venous catheter use, corticosteroid use, antibiotic use for ≥7 days, blood transfusion, and ICU stay duration were 0.754, 0.867, 0.867, 0.680, 0.727, 0.613, 0.616, 0.571, 0.601, and 0.762, respectively. The combined AUC for predicting postoperative fungal infections was 0.992, with a 95% confidence interval of 0.986~0.999, sensitivity of 0.966, and specificity of 0.984, which showed a significant difference compared to Az=0.5 (P<0.05). Conclusion: Candida albicans is the most common fungal strain causing secondary infections following surgery for severe trauma. Fungi show lower sensitivity to itraconazole and higher sensitivity to flucytosine and amphotericin B. Important factors influencing postoperative fungal infections include ISS score, SOFA score at admission, duration of invasive ventilation, use and duration of urinary catheters, use of central venous catheters, use of corticosteroids, blood transfusions, and ICU stay duration. 2024 Vol. 30 (10): 1653-1658 [Abstract] ( 20 ) HTML (1 KB)  PDF (1464 KB)  ( 20 ) 1659 Influence of High-intensity Focused Ultrasound Combined with Leuprorelin and Mirena on Sex Hormones CA125 and AMH Levels in Patients with Adenomyosis TANG Qiulin, TANG Chendian, CHEN Guiqing DOI: 10.3969/j.issn.1006-6233.2024.10.014 Objective: To investigate the influence of high-intensity focused ultrasound (HIFU) combined with leuprorelin and Mirena (levonorgestrel intrauterine system) on the levels of sex hormones, cancer antigen (CA125) and anti-mullerian hormone (AMH) in patients with adenomyosis (AM). Methods: A total of 182 patients with AM were enrolled in this study from June 2021 to June 2022. They were divided into HIFU group (n=20, receiving HIFU treatment, refusing to inject leuprorelin and placing Mirena) and HIFU + leuprorelin group (n=61, receiving HIFU combined with leuprorelin treatment, and refusing to place Mirena), HIFU + leuprorelin + Mirena group (n=101, receiving HIFU combined with leuprorelin and Mirena treatment). Three groups of patients were treated with HIFU once and followed up for 2 years. The clinical efficacy, sex hormones levels [estradiol (E2), luteinizing hormone (LH), follicle stimulating hormone (FSH)], CA125, AMH, C-reactive protein (CRP), uterine volume, menstrual volume, endometrial thickness and pain score (VAS) before and after treatment and incidence rates of adverse reactions during treatment were compared among the three groups. Results: The HIFU + leuprorelin + Mirena group had higher total clinical effective rate after treatment than the HIFU + leuprorelin group and HIFU group (P<0.05). Before treatment, the E2, LH and FSH levels among the three groups were not statistically different (P>0.05). The levels of E2, LH and FSH in the three groups were obviously declined after treatment than those before treatment (P<0.05). After treatment, the differences of E2, LH and FSH were greater in HIFU + leuprorelin + Mirena groupthan those in HIFU + leuprorelin group and HIFU group, and were greater in HIFU + leuprorelin group than those in HIFU group (P<0.05). The levels of CA125, CRP and AMH were not statistically different among the three groups before treatment (P>0.05). After treatment, the CA125 and CRP levels in the three groups were lowered significantly (P<0.05), but the AMH level was not obviously changed after treatment (P>0.05). The differences of CA125 and CRP in HIFU+ leuprorelin + Mirena group after treatment were greater than those in HIFU+ leuprorelin and HIFU groups, and the differences in HIFU+ leuprorelin group were greater than those in HIFU group (P<0.05). Before treatment, no statistical differences were shown in uterine volume, menstrual volume, endometrial thickness and VAS score among the three groups (P>0.05). After treatment, the uterine volume, menstrual volume, endometrial thickness and VAS score were significantly reduced in the three groups compared with those before treatment (P<0.05). The differences of uterine volume, menstrual volume, endometrial thickness and VAS score in HIFU+ leuprorelin + Mirena group after treatment were greater than those in HIFU+ leuprorelin group and HIFU group, and were greater in HIFU+ leuprorelin group than those in HIFU group (P<0.05). During treatment, there were no statistical differences in the incidence rates of adverse reactions among the three groups (P>0.05). After 2 years of follow-up, 3 patients in HIFU group had recurrence symptoms, mainly manifested as increased menstrual volume and aggravated dysmenorrhea. The efficacy in HIFU + leuprorelin + Mirena group was higher than that in HIFU + leuprorelin group and HIFU group (P<0.05). Conclusion: HIFU combined with leuprorelin and Mirena can enhance the therapeutic effect of AM patients, effectively regulate the levels of sex hormones and serum indicators, reduce the uterine volume and endometrial thickness, lower the menstrual volume, and relieve the pain, and it has no significant damage to ovarian reserve function, with good safety. 2024 Vol. 30 (10): 1659-1665 [Abstract] ( 16 ) HTML (1 KB)  PDF (1245 KB)  ( 6 ) 1666 Study on the Relationship Between PLR NLR CA199 and Prognosis in Advanced Gastric Cancer Patients Undergoing Immunotherapy WU Linlin, TANG Lei, ZHANG Yan, et al DOI: 10.3969/j.issn.1006-6233.2024.10.015 Objective: To investigate the relationship between platelet-to-lymphocyte ratio (PLR), neutrophil-to-lymphocyte ratio (NLR), carbohydrate antigen 199 (CA199), and prognosis in advanced gastric cancer patients undergoing immune checkpoint inhibitor (ICI) therapy. Methods: A retrospective analysis was conducted on 103 advanced gastric cancer patients who received ICI treatment at our hospital from January 2022 to March 2024, with follow-up until June 2024. Pre-treatment PLR, NLR, and CA199 levels were collected. The ROC curves for these indicators were plotted against disease progression, and cutoff values were determined. Patients were divided into high and low PLR, NLR, and CA199 groups based on these values. Kaplan-Meier survival curves were plotted for each group, and the Cox proportional hazards regression model was used to analyze factors influencing the median progression-free survival (PFS) of these patients. Results: The ROC curve analysis identified cutoff values for PLR, NLR, and CA199 as 252.47, 3.39, and 43.63 U/mL, respectively. Based on these cutoff values, patients were divided into high PLR (n=58), low PLR (n=45), high NLR (n=50), low NLR (n=53), high CA199 (n=49), and low CA199 (n=54) groups. Kaplan-Meier analysis showed no statistically significant difference in PFS between the high PLR group (8.8 months) and the low PLR group (11.1 months) (P>0.05). However, the high NLR group (7.2 months) and high CA199 group (7.8 months) had significantly shorter PFS compared to the low NLR group (11.9 months) and low CA199 group (11.6 months) (P<0.05). Univariate Cox regression analysis showed that age ≥65 years, ECOG PS ≥1, high PLR, high NLR, and high CA199 were associated with shorter PFS in advanced gastric cancer patients undergoing ICI therapy (P<0.05). Multivariate Cox regression analysis identified ECOG PS ≥1, high NLR, and high CA199 as independent risk factors for PFS in these patients (P<0.05). Conclusion: Pre-treatment PLR, NLR, and CA199 levels are associated with the prognosis of advanced gastric cancer patients receiving ICI therapy, with NLR and CA199 being independent risk factors for PFS. Monitoring these indicators may provide clinical guidance for the treatment of advanced gastric cancer. 2024 Vol. 30 (10): 1666-1670 [Abstract] ( 16 ) HTML (1 KB)  PDF (1639 KB)  ( 9 ) 1671 Effect of Cold Lateral Condensation and Warm Vertical Condensation on NRS Score Masticatory Function and Complications in the Treatment of Pulpitis and Periapical Disease XIA Honghong, LIU Wenjing, QIN Kun, et al DOI: 10.3969/j.issn.1006-6233.2024.10.016 Objective: To observe the effects of cold lateral condensation (CLC) and warm vertical condensation (WVC) on scores of Numerical Rating Scale (NRS), masticatory function and complications in pulposis and periapical disease. Methods: A total of 115 patients with pulposis and periapical disease treated in the hospital were enrolled between January 2021 and December 2023. According to different filling materials, they were divided into a control group (55 cases, CLC) and an experimental group (60 cases, WVC). The clinical effect and filling effect after treatment, scores of NRS, bite force, masticatory function and periodontal status [gingival index (GI), sulcus bleeding index (SBI)] before and after treatment, and the occurrence of complications were compared between the two groups. Results: After treatment, total clinical response rate in the experimental group was higher than that in the control group (96.67% vs 83.74%, P<0.05). After treatment, proportion of appropriate filling in the experimental group was higher than that in the control group (91.67% vs 65.45%, P<0.05), and difference value of NRS score before and after treatment was greater than that in control group (2.54±0.41 points vs 1.38±0.56 points, P<0.05). After treatment, difference values in scores of bite force and masticatory function in the experimental group were greater than those in the control group (49.22±7.79lbs, 10.86±1.05 points vs 26.53±3.61lbs, 5.34±0.59 points, P<0.05), and difference values in scores of GI and SBI were greater than those in control group (1.33±0.15 points, 1.04±0.13 points vs 1.05±0.14 points, 0.88±0.08 points, P<0.05). After treatment, incidence of complications in the experimental group was lower than that in the control group (P<0.05). Conclusion: Compared with CLC, WVC can improve clinical curative effect and root canal filling effect, relieve pain, promote the recovery of dental practicability and periodontal status in patients with pulposis and periapical disease, which has low incidence of complications. 2024 Vol. 30 (10): 1671-1675 [Abstract] ( 10 ) HTML (1 KB)  PDF (1174 KB)  ( 5 ) 1676 Correlation between Serum IL-35 and CCL19 Levels and Immunological Indexes in Children with Systemic Lupus Erythematosus ZHOU Xiaoqian, ZHU Hongtao, AIGULI Abbas, et al DOI: 10.3969/j.issn.1006-6233.2024.10.017 Objective: To observe the changes of serum interleukin-35 (IL-35) and C-C motif chemokine ligand 19 (CCL19) in children with systemic lupus erythematosus (SLE), and to analyze the association with immunological indexes. Methods: A total of 97 children with SLE in the hospital were included from January 2022 to March 2024, and 97 healthy children were selected for control study during the same time period. The changes of serum IL-35 and CCL19 levels were observed, and the correlation with immunological indexes [including anti-ds-DNA antibody, immunoglobulin (Ig), complements C3 and C4] was analyzed. Results: Compared with the healthy children, the children with SLE had a significantly lower serum IL-35 level and a significantly higher serum CCL19 level (P<0.05). Compared with the children with inactive SLE, serum levels of IL-35, C3, and C4 in the children with active SLE were significantly lower while serum levels of CCL19, anti-ds-DNA antibody, IgM, IgG, and IgA were significantly higher (P<0.05). Pearson correlation analysis showed that the serum level of IL-35 in children with SLE was negatively correlated with the levels of anti-ds-DNA antibody, IgM, IgG, and IgA, and was positively correlated with the levels of C3 and C4 (P<0.05). Serum CCL19 level was positively correlated with the levels of anti-ds-DNA antibody, IgM, IgG and IgA, and was negatively correlated with the levels of C3 and C4 in children with SLE (r=-0.314, -0.203, -0.301, -0.201, 0.318, 0.383, P<0.05). Linear regression analysis suggested that anti-ds-DNA antibody had a negative impact on IL-35 level in children with SLE (r=0.272, 0.209, 0.362, 0.202, -0.219, -0.391,P<0.05) while C3 had a positive impact on IL-35 level (P<0.05). IgG in children with SLE had a positive impact on CCL19 level (P<0.05). Conclusion: The abnormal changes of serum IL-35 and CCL19 levels in children with SLE are correlated with the levels of anti-ds-DNA antibody, IgM, IgG and IgA. There is a certain influence relationship between anti-ds-DNA antibody, C3 and IL-35, IgG and CCL19. 2024 Vol. 30 (10): 1676-1679 [Abstract] ( 14 ) HTML (1 KB)  PDF (1170 KB)  ( 6 ) 1680 Efficacy and Safety Analysis of Different Power HoLEP Combined with Holmium Laser Lithotripsy in the Treatment of Benign Prostatic Hyperplasia with Bladder Stones XIA Dongdong, ZENG Minghui, QIN Suobing, et al DOI: 10.3969/j.issn.1006-6233.2024.10.018 Objective: To study the efficacy and safety of different powers of holmium laser enucleation of the prostate (HoLEP) combined with holmium laser cystolithotripsy (HLC) in the treatment of benign prostatic hyperplasia (BPH) with bladder stones. Methods: The clinical data of 110 patients with BPH combined with bladder stones admitted to Danyang People's Hospital from January 2019 to January 2024 were collected. According to the surgical methods, patients who received high-power HoLEP combined with HLC were included in the HP group (n=62), and patients who adopted low-power HoLEP combined with HLC were enrolled as the LP group (n=48). Perioperative indicators, urinary function, stress indicators, and postoperative complications were compared between both groups. Results: The surgical time, excised tissue mass, bladder irrigation time and postoperative urinary catheter retention time with (68.49±15.36) min, (41.42±10.59) g (1.86±0.66) d and (2.34±0.85) d in the HP group were not statistically different form (70.15±15.71) min, (42.81±9.93) g, (1.81±0.72) d and (2.58±0.92) d in the LP group (P>0.05). The time of glandular enucleation and hospital stay with (35.80±7.21) min and (3.62±0.64) d in the HP group were shorter than (41.64±7.83) min and (3.86±0.50) d in the LP group, and the enucleation efficiency and decrease rate of hemoglobin with (1.15±0.25) g/min and (8.05±1.58) g/L were higher than (1.02±0.18) g/min and (7.36±1.72) g/L in the LP group (P<0.05). At 3 months after surgery, the differences of Qmax, PVR and PSA before and after treatment were not statistically significant between the HP group and the LP group (P>0.05). The differences of SP, PGE2 and NE before and after treatment revealed no statistical differences between the HP group and the LP group at 24 hours after surgery (P>0.05). There was no statistical significance in incidence of postoperative complications between the HP group (12.88%) and the LP group (10.40%) (P>0.05). Conclusion: High-power and low-power HoLEP combined with HLC have similar efficacy in the treatment of BPH with bladder stones. High-power HoLEP has higher enucleation efficiency, and shorter glandular enucleation time and hospital stay, and low-power HoLEP has better hemostatic effect. 2024 Vol. 30 (10): 1680-1684 [Abstract] ( 20 ) HTML (1 KB)  PDF (1203 KB)  ( 6 ) 1685 Correlation between CTRP-3 CTRP-12 Levels and GOLD Grading and Prognosis of Chronic Obstructive Pulmonary Disease WANG Ming, DING Guozheng, LV Zhifang DOI: 10.3969/j.issn.1006-6233.2024.10.019 Objective: To analyze the correlation between levels of C1q tumor necrosis factor-related protein 3 (CTRP-3) and C1q tumor necrosis factor-related protein 12 (CTRP12) and global initiative (GOLD) grading and prognosis of chronic obstructive pulmonary disease (COPD). Methods: A total of 126 patients with COPD in the hospital were selected from September 2022 to January 2024 and included in the case group, and 90 healthy volunteers receiving physical examination were assigned into the control group. The clinical data and serum CTRP-3 and CTRP12 levels were compared between both groups. According to the GOLD grading, COPD patients were classified into grade I group (n=9), grade Ⅱ group (n=21), grade Ⅲ group (n=43) and grade IV group (n=53). The levels of CTRP-3 and CTRP12 among the four groups were compared. The prognosis in the case group was recorded, and the CTRP-3 and CTRP12 levels were compared among patients with different prognosis status. Pearson correlation analysis was adopted to explore the correlation between clinical indicators and levels of serum CTRP-3 and CTRP12. Results: The age, gender, body mass index, smoking history and underlying diseases between the two groups manifested no statistical differences (P>0.05). WBC in the case group was higher (P<0.05) while FEV1 and FEV1/FVC were lower than those in the control group (P<0.05). The levels of CTRP-3 and CTRP12 were lower in the case group (P<0.05). There were statistical differences in the levels of CTRP-3 and CTRP12 among COPD patients with different GOLD grades (P<0.05). CTRP-3 and CTRP12 levels were manifested as grade I group>grade Ⅱ group>grade Ⅲ group>grade IV group (P<0.05). Compared with the poor prognosis group, the levels of CTRP-3 and CTRP12 in the good prognosis group were higher (P<0.05). Correlation analysis showed that serum CTRP-3 level in COPD patients was negatively correlated with smoking history and GOLD grading (P<0.05), and was positively associated with FEV1/FVC (P<0.05). CTRP12 level was negatively correlated with smoking history, WBC and GOLD grading (P<0.05), and was positively correlated with FEV1/FVC (P<0.05). The levels of CTRP-3 and CTRP12 were not correlated with the duration of COPD, hospital stay and FEV1 (P>0.05). Conclusion: Serum levels of CTRP-3 and CTRP12 in COPD patients are lower compared with those of healthy people, and are negatively correlated with smoking history and GOLD grading, and positively correlated with FEV1/FVC level. 2024 Vol. 30 (10): 1685-1689 [Abstract] ( 16 ) HTML (1 KB)  PDF (1183 KB)  ( 6 ) 1690 Impact of Low-Dose Chemotherapy Combined with Postoperative Radiotherapy on the Prognosis of Stage Ⅲ Ⅳ Oral Cancer Patients and Analysis of Related Factors ZHU Ling, DU Ruohong, HOU Jun DOI: 10.3969/j.issn.1006-6233.2024.10.020 Objective: To observe the impact of low-dose chemotherapy combined with postoperative radiotherapy on the prognosis of patients with stage Ⅲ-Ⅳ oral cancer and to analyze the factors affecting prognosis. Methods: A total of 90 patients with stage Ⅲ~Ⅳ oral cancer, treated at our hospital between January 2019 and May 2022, were selected and randomly divided into two groups: the control group (n=45) received postoperative radiotherapy, and the combined group (n=45) received low-dose preoperative chemotherapy combined with postoperative radiotherapy. Clinical efficacy and the 2-year recurrence rates were compared between the two groups. Based on the patients' 2-year postoperative recurrence status, they were divided into the recurrence group and the non-recurrence group, and a multivariate logistic regression analysis was conducted to identify factors influencing prognosis. Results: The disease control rate (DCR) of the combined group was significantly higher than that of the control group (P<0.05). After a 2-year follow-up, 3 patients from the combined group and 4 from the control group were lost to follow-up. Among the 83 patients who completed follow-up, 39 experienced recurrence, with an overall recurrence rate of 46.99% (39/83). The recurrence rate in the combined group was 40.48% (17/42), compared to 53.66% (22/41) in the control group, with no statistically significant difference between the two groups (P>0.05). Significant differences were observed between the recurrence and non-recurrence groups in terms of pN stage, pathological differentiation, lymph node ratio, and nerve invasion (P<0.05). Multivariate analysis showed that pN stage, lymph node ratio, and nerve invasion were influencing factors for postoperative recurrence in patients with stage Ⅲ-Ⅳ oral cancer (P<0.05). Conclusion: Low-dose chemotherapy combined with postoperative radiotherapy improves clinical efficacy in patients with stage Ⅲ-Ⅳ oral cancer, but has no significant advantage in improving prognosis. Prognosis-related factors include pN stage, lymph node ratio, and nerve invasion. 2024 Vol. 30 (10): 1690-1694 [Abstract] ( 17 ) HTML (1 KB)  PDF (1162 KB)  ( 8 ) 1695 Methylation of SOX17 Gene in Plasma Circulating DNA and Its Correlation with Disease Status and Prognosis in Patients with Papillary Thyroid Carcinoma CHAO Xiangsong, SUN Jiabin, HOU Yinglong DOI: 10.3969/j.issn.1006-6233.2024.10.021 Objective: To explore the relationship between SOX17 gene methylation in plasma circulating DNA and the disease condition and prognosis of patients with papillary thyroid carcinoma (PTC). Methods: Thyroid cancer tissue and preoperative blood samples were collected from 152 PTC patients who underwent surgical resection between June 2022 and December 2023. Methylation-specific PCR (MSP) was used to detect SOX17 promoter methylation in PTC tissues and plasma circulating DNA. Results: MSP results showed that 71 out of 152 PTC tissue samples (46.7%) and 76 plasma circulating DNA samples (50.0%) exhibited SOX17 methylation. Spearman correlation analysis indicated a significant correlation between SOX17 methylation in plasma circulating DNA and PTC tissues (r=0.382, P<0.001). SOX17 methylation in PTC tissue and plasma circulating DNA was significantly associated with tumor stages Ⅱ~Ⅲ, tumor size ≥2cm, and lymph node metastasis (LNM) (P<0.05). Furthermore, SOX17 methylation in plasma circulating DNA was significantly related to the occurrence of extrathyroidal extension (ETE) (P<0.05). SOX17 methylation in plasma circulating DNA was identified as an independent factor influencing LNM and ETE in PTC patients (OR=9.564, 2.744; 95% CI=3.875~23.602, 1.109~6.792, P<0.05). The predictive ability of SOX17 methylation in plasma circulating DNA for LNM and ETE was relatively high, with an area under the curve (AUC) of 0.731 and 0.630 (sensitivity 74.0%, 65.6%; specificity 72.2%, 60.4%). In contrast, the predictive ability of SOX17 methylation in PTC tissues for LNM and ETE was lower, with an AUC of 0.604 and 0.562 (sensitivity 57.5%, 54.1%; specificity 63.3%, 58.2%). Conclusion: SOX17 promoter methylation in tumor tissue and plasma DNA exhibits high consistency during the occurrence and progression of PTC. Moreover, SOX17 promoter methylation in plasma DNA has a higher predictive value for LNM and ETE. 2024 Vol. 30 (10): 1695-1699 [Abstract] ( 15 ) HTML (1 KB)  PDF (1220 KB)  ( 5 ) 1700 The Relationship between IL-23/IL-17 Axis in Peripheral Blood and Disease Activity in Patients with Rheumatoid Arthritis and Its Predictive Value for Bone Destruction Progression MA Zhongshuang, ZHU Honghong, LIU Ying DOI: 10.3969/j.issn.1006-6233.2024.10.022 Objective: To investigate the relationship between the interleukin-23 (IL-23)/interleukin-17 (IL-17) axis in the peripheral blood of patients with rheumatoid arthritis (RA) and disease activity, and its predictive value for the progression of bone destruction. Methods: A total of 118 RA patients were enrolled between May 2022 and October 2023. They were divided into mild, moderate, and severe disease activity groups according to the Disease Activity Score 28 (DAS28) and received symptomatic treatment based on their condition. Patients were followed up for 6 months, and were further divided into a progression group (27 cases) and a non-progression group (91 cases) based on the presence of bone destruction progression. Fasting venous blood (3 mL) was collected from each patient on the morning after admission, and the levels of IL-23/IL-17 axis factors [IL-23, IL-17, interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α)] were measured using enzyme-linked immunosorbent assay (ELISA). The correlation between IL-23/IL-17 axis factors and disease activity was analyzed, and the predictive value of these factors for bone destruction progression was evaluated. Results: The disease course and disease activity in the progression group were significantly higher than those in the non-progression group (P<0.05). The levels of IL-23, IL-17, IL-1β, IL-6, and TNF-α in the peripheral blood of the progression group [(192.38±48.72) pg/mL, (31.13±7.24) pg/mL, (88.71±29.10) pg/mL, (18.59±4.21) pg/mL, (180.31±41.57) ng/L] were higher than those in the non-progression group [(149.07±42.15) pg/mL, (25.04±5.39) pg/mL, (56.92±16.52) pg/mL, (15.16±3.44) pg/mL, (142.59±36.62) ng/L] (P<0.05). The levels of IL-23, IL-17, IL-1β, IL-6, and TNF-α in the peripheral blood showed a progressive increase from patients with mild to moderate to severe disease activity (P<0.05). The levels of IL-23, IL-17, IL-1β, IL-6, and TNF-α were positively correlated with disease activity (P<0.05). Partial correlation analysis showed that after excluding the influence of disease course and disease activity, IL-23, IL-17, IL-1β, IL-6, and TNF-α remained significantly correlated with the progression of bone destruction in RA patients (P<0.05). The area under the curve (AUC) values for IL-23, IL-17, IL-1β, IL-6, and TNF-α in predicting the progression of bone destruction in RA patients were 0.807, 0.804, 0.793, 0.792, and 0.794, with sensitivities of 81.48%, 74.07%, 74.07%, 85.19%, and 66.67%, and specificities of 70.33%, 75.82%, 73.63%, 71.43%, and 80.22%, respectively. The combined prediction of bone destruction progression had an AUC of 0.931, with a sensitivity of 85.19% and specificity of 87.91%, which was significantly superior to the individual predictions (Z=2.715, 2.803, 3.026, 3.114, 2.984, all P<0.001). Conclusion: The IL-23/IL-17 axis in peripheral blood is significantly associated with disease activity in RA patients and has a reliable predictive value for the progression of bone destruction. 2024 Vol. 30 (10): 1700-1704 [Abstract] ( 11 ) HTML (1 KB)  PDF (1352 KB)  ( 5 ) 1705 Risk Model Construction for Adverse Prognosis in Chronic Heart Failure LIANG Hao, et al DOI: 10.3969/j.issn.1006-6233.2024.10.023 Objective: To analyze the factors related to poor prognosis in chronic heart failure (CHF) and construct a risk model for poor prognosis. Methods: A retrospective analysis was conducted on 216 CHF patients. Patients were divided into good prognosis and poor prognosis groups based on the NYHA classification. Baseline characteristics and admission indicators were compared between the two groups. Logistic regression analysis was used to identify independent risk factors associated with adverse prognosis. Results: Patients in the poor prognosis group were older, had longer hospital stays, and higher NYHA classes. They also had lower weight, systolic blood pressure, and levels of potassium, sodium, and chloride. Multivariate logistic regression analysis showed that older age, longer hospital stay, and higher NYHA class were independent risk factors for adverse prognosis, while higher systolic blood pressure, weight, sodium, chloride, and potassium levels were protective factors. A risk model based on sodium levels had a high predictive value for adverse prognosis. Conclusion: The established risk model can be used to predict adverse outcomes in CHF patients. Early identification of high-risk patients and targeted interventions can improve the prognosis of CHF patients. 2024 Vol. 30 (10): 1705-1710 [Abstract] ( 19 ) HTML (1 KB)  PDF (1391 KB)  ( 10 ) 1711 Quantitative EEG Features Associated with Poor Prognosis in Patients with Hypertensive Intracerebral Hemorrhage OU Ya, ZHANG Pingshu, YUAN Xiaodong, et al DOI: 10.3969/j.issn.1006-6233.2024.10.024 Objective: To investigate the quantitative EEG (qEEG) features associated with poor prognosis in patients with hypertensive intracerebral hemorrhage (HICH). Methods: A prospective study was conducted, continuously enrolling 69 patients with HICH from the intensive care unit of the Department of Neurology, Kailuan General Hospital, between January 2017 and June 2023. Based on the modified Rankin score (mRS) at 30 days post-discharge, patients were divided into two groups: the good prognosis group (mRS ≤ 2) and the poor prognosis group (mRS 3-6). General patient data and qEEG monitoring parameters, including alpha, theta, delta, and beta wave power values in the left and right frontal (Fp1, Fp2), frontal (F3, F4), central (C3, C4), parietal (P3, P4), occipital (O1, O2), anterior temporal (F7, F8), mid-temporal (T3, T4), and posterior temporal (T5, T6) regions, were collected. Results: The mean age in the poor prognosis group was significantly higher than in the good prognosis group (P<0.05). The delta wave power in the Fp2, F4, C4, P4, F7-8, and T4 regions in the poor prognosis group was significantly higher than in the good prognosis group (P<0.05). The theta wave power in the Fp1-2, F3, C3, P4, O1, F7, and T3 regions in the poor prognosis group was significantly higher than in the good prognosis group (P<0.05). The overall slow wave index power, including delta and theta waves, in the Fp1-2, F3-4, C3-4, P3-4, O1, F7-8, T4, and whole-brain average slow wave index in the poor prognosis group was significantly higher than in the good prognosis group (P<0.05). Conclusion: Patients with poor prognosis following hypertensive intracerebral hemorrhage show significantly increased delta, theta, and whole-brain average slow wave index power values on qEEG. 2024 Vol. 30 (10): 1711-1717 [Abstract] ( 16 ) HTML (1 KB)  PDF (1185 KB)  ( 4 ) 1718 Experimental Study on the Effect of Silencing HIF-1α on the Growth of Laryngeal Cancer Cells by Regulating SLC7A11 LIU Jianwei, LIU Pengchao, HU Jinhong, et al DOI: 10.3969/j.issn.1006-6233.2024.10.025 Objective: To explore the specific effect of silencing HIF-1α on SLC7A11 in laryngeal cancer, and to find new tumor markers and potential therapeutic targets for clinical diagnosis of laryngeal cancer. Methods: UMSCC5 laryngeal cancer cells were cultured and transfected with HIF-1α-siRNA. Western blot was used to screen the best transfection efficiency. CCK-8 was used to detect the proliferation activity of the transfected cells. Flow cytometry was used to detect the apoptosis of each group. Transwell method was used to detect cell invasion and migration. Western blot was used to detect the expression of HIF-1α and SLC7A11. Results: The expression of HIF-1α was successfully silenced, and the transfection efficiency of HIF-1α-siRNA1 group was the highest and the most stable ( P<0.05 ). Silencing HIF-1α can significantly inhibit the proliferation of human laryngeal carcinoma UMSCC5 cells ( P<0.05 ), promote apoptosis ( P<0.05 ), and inhibit the invasion and migration of laryngeal carcinoma cells ( P<0.05 ). Silencing HIF-1α could significantly down-regulate the expression of SLC7A11 protein ( P<0.05 ). Conclusion: Silencing HIF-1α can inhibit the proliferation, invasion, migration and apoptosis of human laryngeal carcinoma UMSCC5 cells, and inhibit the expression of SLC7A11. It provides new ideas and theoretical basis for clinical molecular targeted therapy of laryngeal cancer based on HIF-1α in the future. 2024 Vol. 30 (10): 1718-1721 [Abstract] ( 14 ) HTML (1 KB)  PDF (1827 KB)  ( 7 ) 1722 Relationship between NEU/ALB Hemodynamics, Immunoglobulins and Renal Function Outcome in Elderly Patients with Infection-Induced Acute Kidney Injury XIE Juan, NI Gang, PAN Yachao, et al DOI: 10.3969/j.issn.1006-6233.2024.10.026 Objective: To investigate the relationship of neutrophil (NEU)/albumin (ALB), hemodynamics and immunoglobulins levels with renal function outcome in elderly patients with infection-induced acute kidney injury (AKI). Methods: Totally 117 elderly patients with infection-induced AKI in the hospital were selected from January 2021 to December 2023 as the research subjects. NEU, ALB, hemodynamics and immunoglobulin were detected at admission. The patients were treated with comprehensive symptomatic treatment and were divided into good outcome group and poor outcome group according to the outcome of renal function. The levels of NEU/ALB, hemodynamics and immunoglobulins were compared between groups. Multivariate Logistic regression analysis was used to analyze the influencing factors of renal function outcome in elderly patients with infection-induced AKI. Results: There were no statistical differences in gender, age, body mass index (BMI) and urinary protein between the good outcome group and the poor outcome group (P>0.05), but serum creatinine level was lower than that in the poor outcome group (P<0.05). NEU and NEU/ALB in the good outcome group were lower than those in the poor outcome group (P<0.05) while ALB, IgG and IgM levels were higher (P<0.05), and MAP and CI were higher than those in the poor outcome group (P<0.05), but the HR and IgA were not statistically different compared with those in the poor outcome group (P>0.05). Multivariate Logistic regression analysis showed that NEU/ALB, MAP, CI and IgG were influencing factors of renal function outcome in elderly patients with infection-induced AKI (P<0.05). Conclusion: The NEU/ALB, hemodynamics and immunoglobulins levels are correlated with renal function outcome in elderly patients with infection-induced acute kidney injury. The detection of the above indicators is helpful to evaluate the renal function outcome of patients. 2024 Vol. 30 (10): 1722-1726 [Abstract] ( 12 ) HTML (1 KB)  PDF (1194 KB)  ( 3 ) 1727 Effect of Polyene Phosphatidylcholine Injection Combined with Adenosylmethionine Butanedisulfonate on Liver Function, Albumin, and Coagulation Indicators in Patients with Liver Failure YE Chong, WU Qianqian, LIU Changwei, et al DOI: 10.3969/j.issn.1006-6233.2024.10.027 Objective: To investigate the effects of polyene phosphatidylcholine injection combined with adenosylmethionine butanedisulfonate on liver function, albumin, and coagulation indicators in patients with liver failure. Methods: A total of 107 patients with liver failure treated at The First Affiliated Hospital of Anhui Medical University from December 2021 to December 2023 were selected and randomly divided into a control group (n=53, treated with polyene phosphatidylcholine injection) and a combined group (n=54, treated with polyene phosphatidylcholine injection combined with adenosylmethionine butanedisulfonate). The therapeutic effects, liver function, protein-related indicators, coagulation function, and complications of liver failure were compared between the two groups. Results: The total effective rate in the combined group (94.44%) was higher than in the control group (81.13%), with a statistically significant difference (P<0.05). After 4 weeks of treatment, the differences in alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total bilirubin (TBIL) levels before and after treatment were greater in the combined group [(204.07±22.85) U/L, (151.45±30.16) U/L, (212.10±26.64) μmoL/L] compared to the control group [(189.23±24.70) U/L, (123.03±29.48) U/L, (196.59±23.20) μmoL/L], with statistical significance (P<0.05). The combined group also showed significantly higher improvements in serum total protein (TP), albumin (ALB), and prealbumin (PA) levels [(7.41±2.26) g/L, (8.53±2.08) g/L, (55.24±10.72) mg/L] compared to the control group [(2.64±1.69) g/L, (4.56±1.75) g/L, (36.18±11.82) mg/L] (P<0.05). Additionally, fibrinogen (FIB), prothrombin activity (PTA), and D-dimer (D-D) improvements in the combined group [(0.55±0.17) g/L, (24.89±3.92)%, (1.21±0.44) mg/L] were greater than those in the control group [(0.34±0.20) g/L, (18.33±4.06)%, (1.02±0.35) mg/L] (P<0.05). The incidence of complications of liver failure was not significantly different between the two groups (P>0.05). Conclusion: The combination of polyene phosphatidylcholine injection and adenosylmethionine butanedisulfonate improves liver and coagulation function in patients with liver failure. The combined treatment is more effective than monotherapy. 2024 Vol. 30 (10): 1727-1732 [Abstract] ( 13 ) HTML (1 KB)  PDF (1180 KB)  ( 4 ) 1733 Efficacy of Protamine Recombinant Human Insulin Mix 40/60 in Treating Elderly Patients with Type 2 Diabetes and Its Impact on Prognosis XIAO Yao, PAN Yinhong, MA Lihong, et al DOI: 10.3969/j.issn.1006-6233.2024.10.028 Objective: To explore the efficacy of protamine recombinant human insulin mix 40/60 (referred to as Gansulin 40/60) in treating elderly patients with type 2 diabetes (T2DM) and its impact on prognosis. Methods: A total of 106 elderly T2DM patients admitted to Western Theater General Hospital between December 2022 and December 2023 were randomly divided into a control group (n=53) and a treatment group (n=53). The control group received standard dual antidiabetic therapy (metformin + dapagliflozin), while the treatment group was given Gansulin 40/60 subcutaneous injections. After 12 weeks, efficacy and safety were evaluated, and pre- and post-treatment glycemic indicators, body mass index (BMI), β-cell function, and serum levels of monocyte chemoattractant protein-1 (MCP-1), high-sensitivity C-reactive protein (hs-CRP), and interleukin-6 (IL-6) were measured. The prognosis of both groups was tracked for 6 months. Results: The overall effective rate in the treatment group was 90.57%, higher than that in the control group (71.70%) (P<0.05). After treatment, the treatment group showed lower fasting plasma glucose (FPG), 2-hour postprandial glucose (2hPG), glycosylated hemoglobin (HbA1c), homeostasis model assessment of insulin resistance (HOMA-IR), and serum MCP-1, hs-CRP, and IL-6 levels, and higher β-cell function index (HOMA-β) compared to the control group (P<0.05). The overall incidence of adverse reactions in the treatment group (3.77%) was not significantly different from that in the control group (7.55%) (P>0.05). There was no significant difference in the incidence of microvascular complications between the two groups over the 6-month follow-up period (P>0.05). Conclusion: Subcutaneous injection of Gansulin 40/60 in elderly T2DM patients is safe and effective, lowering blood glucose levels, promoting β-cell function recovery, and reducing inflammation, though it does not significantly improve short-term prognosis. 2024 Vol. 30 (10): 1733-1738 [Abstract] ( 26 ) HTML (1 KB)  PDF (1178 KB)  ( 5 ) 1739 Evaluation of the Efficacy and Safety of Budesonide Combined with Interferon α2b Nebulization in the Treatment of Infantile Bronchopneumonia YUAN Bin, CAI Jinbiao, WANG Juan, et al DOI: 10.3969/j.issn.1006-6233.2024.10.029 Objective: To evaluate the efficacy and safety of budesonide combined with interferon α2b nebulization in the treatment of infantile bronchopneumonia. Methods: A total of 102 infants and young children with bronchopneumonia admitted to our hospital from 2021.09 to 2023.07 were enrolled. According to the random number table method, they were divided into the conventional group (conventional symptomatic treatment + budesonide atomization inhalation) and combined group (combined with interferon α2b atomization inhalation on the basis of the conventional group), with 51 cases in each group. The clinical efficacy, improvement time of symptoms (cough, wheezing, wheezing rale, three concave signs), blood gas indexes [oxygenation index, partial pressure of oxygen (PaO2), partial pressure of carbon dioxide (PaCO2)], T lymphocyte indicators (CD3+CD4+, CD3+CD8+, CD3-CD19+) and peripheral blood inflammation indexes [Interleukin-6 (IL-6), tumor necrosis factor α (TNF-α), C-reactive protein (CRP)] before and after treatment were compared between the two groups, and the adverse drug reactions were compared. Results: The total effective rate of the combined group (98.40%) was higher than that of the conventional group (86.27%) ((P<0.05). The improvement time of symptoms in the combined group was shorter than that in the conventional group (P<0.05). Before treatment, there were no statistical differences in serum indexes between groups (P>0.05). After treatment, the oxygenation index and PaO2 in the two groups were increased (P<0.05) while the PaCO2 was decreased (P<0.05), and the differences before and after treatment in the combined group were higher (P<0.05). No statistical differences were shown in T lymphocyte indexes between groups before treatment (P>0.05). After treatment, CD3+CD4+and CD3+CD8+ in both groups of children were risen (P<0.05) while CD3-CD19++ was declined (P<0.05), and the differences before and after treatment were higher in the combined group than those in the conventional group (P<0.05). Before treatment, there were no statistical differences in peripheral blood inflammation indexes between groups (P>0.05). After treatment, the peripheral blood inflammatory indexes (IL-6, TNF-α, CRP) in the two groups were significantly decreased (P<0.05), and the differences before and after treatment in the combined group were higher than those in the conventional group (P<0.05). There was no statistical significance in the total incidence rate of adverse drug reactions between the combined group (7.84%) and the conventional group (5.88%) (P>0.05). Conclusion: Budesonide combined with interferon α2 b atomization inhalation is beneficial to improve the clinical symptoms, lung function and immune inflammatory response of infants and young children with bronchopneumonia, and improve the efficacy, with high safety. 2024 Vol. 30 (10): 1739-1744 [Abstract] ( 16 ) HTML (1 KB)  PDF (1201 KB)  ( 3 ) 1745 Efficacy of Toripalimab Combined with Lenvatinib and TACE in the Treatment of Advanced Hepatocellular Carcinoma and Its Impact on Tumor Markers and Prognosis WU Xiaolong, et al DOI: 10.3969/j.issn.1006-6233.2024.10.030 Objective: To explore the efficacy of toripalimab combined with lenvatinib and transarterial chemoembolization (TACE) in the treatment of advanced hepatocellular carcinoma (HCC) and analyze its effects on tumor markers and prognosis. Methods: A total of 108 patients with advanced HCC admitted to the Affiliated Hospital of Xiangya School of Medicine, Central South University (Changde First People's Hospital) from January 2022 to March 2023 were selected and randomly divided into two groups: the study group and the control group, with 54 patients in each group. The control group received lenvatinib + TACE treatment, while the study group received toripalimab + lenvatinib + TACE treatment. Each treatment course lasted 21 days, and three consecutive courses were administered. The treatment efficacy and adverse reactions were compared between the two groups after three courses. Tumor markers [Golgi protein 73 (GP73), carcinoembryonic antigen (CEA), tumor-specific growth factor (TSGF), carbohydrate antigen 19-9 (CA19-9)], immune function (T lymphocyte subsets), serum factors [interleukin-1β (IL-1β), alpha-2-HS-glycoprotein (AHSG), nuclear factor kappa B (NF-κB), soluble epidermal growth factor receptor 3 (sErbB3)], quality of life [FACT-G (Functional Assessment of Cancer Therapy-General) score], and functional status [Karnofsky Performance Status (KPS) score] were compared before and after treatment. Prognosis was assessed within one year after treatment in both groups. Results: The objective response rate (ORR) of the study group was significantly higher than that of the control group (59.26% vs. 37.04%, χ2=5.341, P=0.021). After treatment, serum levels of GP73, CEA, TSGF, and CA19-9 in the study group were significantly lower than those in the control group [(198.62±61.79) ng/mL, (20.45±2.48) ng/mL, (62.08±7.12) U/mL, (26.07±3.49) U/mL vs. (237.91±66.35) ng/mL, (25.63±2.79) ng/mL, (70.61±7.59) U/mL, (29.58±3.86) U/mL, P<0.05]. The levels of CD3+, CD4+, and CD4+/CD8+ in the study group were significantly higher than in the control group (P<0.05). Serum levels of IL-1β, AHSG, NF-κB, and sErbB3 in the study group were lower than those in the control group [(15.88±3.64) pg/mL, (9.39±3.13) ng/mL, (175.49±38.62) ng/L, (2791.36±256.84) ng/mL vs. (20.56±4.37) pg/mL, (11.72±3.41) ng/mL, (212.83±43.57) ng/L, (2983.49±268.07) ng/mL, P<0.05]. FACT-G and KPS scores were higher in the study group than in the control group after treatment (P<0.05). There was no significant difference in adverse reactions between the two groups (P>0.05). The 1-year survival rate in the study group was 29.63%, significantly higher than the 18.52% in the control group (P<0.05). Conclusion: Toripalimab combined with lenvatinib and TACE has a significant therapeutic effect on advanced hepatocellular carcinoma. It can reduce tumor marker levels, improve immune function and the tumor microenvironment, enhance the quality of life and functional status, and increase the survival rate, with good safety. 2024 Vol. 30 (10): 1745-1750 [Abstract] ( 13 ) HTML (1 KB)  PDF (1218 KB)  ( 5 ) 1751 Effect of Eramod Combined with Diacreine on TH17/Treg Cytokines in Patients with Refractory Rheumatoid Arthritis ZHANG Xuezhen, ZHUANG Peiying, ZENG Yanfang, et al DOI: 10.3969/j.issn.1006-6233.2024.10.031 Objective: To investigate the efficacy of diacreine combined with eramode in the treatment of refractory rheumatoid arthritis (RRA) and the effects on helper T cell 17 (TH17)/regulatory T lymphocyte (Treg) in patients. Methods: A total of 80 patients with RRA admitted to hospital from January 2021 to December 2023 were retrospectively analyzed and divided into single group (40 cases with eramodide alone) and combined group (40 cases with eramodide and diacerein) according to different treatment methods. Serological indexes [interleukin-17 (IL-17), TH17, Treg, TH17/Treg], serum inflammatory factors [C-reactive protein (CRP), tumor necrosis factor (TNF-α), interleukin-1β (IL-1β), interleukin-1 (IL-1)] and immunological indexes were compared between the two groups before and after treatment [Immunoglobulin] M(IgM), immunoglobulin A(IgA), immunoglobulin G(IgG)], laboratory indicators [human endostatin (ES), rheumatoid factor (RF), erythrocyte deposition rate (ESR)], improvement of clinical symptoms (number of joint swelling, morning stiffness time, joint pain (VAS) score), total response rate and the incidence of adverse reactions (leukopenia, nausea and vomiting, pruritus, and rash) after treatment. Results: Compared with the single group, the combination group had lower IL-17, TH17, TH17/Treg, CRP, TNF-α, IL-1β, IL-1, IgM, IgA, IgG, RF, ESR and joint swelling number, VAS score, higher Treg and ES, and shorter morning stiffness time after treatment. The difference was statistically significant (P<0.05). The total effective rate of combination group (97.50%) was higher than that of single group (80.00%), and the difference was statistically significant (P<0.05). There was no significant difference in the incidence of leukopenia, nausea and vomiting, pruritus and rash between the two groups (P>0.05). Conclusion: In the treatment of RRA, the combination of diacrein and eramod has significant efficacy, which is beneficial to further improve inflammatory factors, immunological indicators and laboratory indicators of patients and alleviate symptoms, and can also correct TH17/Treg imbalance with less adverse reactions. 2024 Vol. 30 (10): 1751-1757 [Abstract] ( 17 ) HTML (1 KB)  PDF (1194 KB)  ( 8 )

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