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2024 Vol. 30, No. 7 Published: 2024-07-31 1057 Effect of Phloretin on the Malignant Progression of Lung Cancer Cells by Regulating the CCL2-CCR2 Signaling Axis JIANG Shasha, YANG Peng, RONG Liwen, et al DOI: 10.3969/j.issn.1006-6233.2024.07.01 Objective: To investigate the effect of phloretin on the CC chemokine ligand 2 (CCL2) - CC chemokine receptor 2 (CCR2) signaling axis on the malignant progression of lung cancer cells. Methods: A549 cells were grouped into a control group (untreated A549 cells), a low-dose phloretin group (500 μmoL/L phloretin treated A549 cells), a medium-dose phloretin group (750μmoL/L phloretin treated A549 cells), a high-dose phloretin group (1000μmoL/L phloretin treated A549 cells), and a high-dose phloretin+GW0742 group (1000μmoL/L phloretin and 1.0μmoL/L CCL2-CCR2 signaling pathway activator GW0742 co-treated A549 cells). CCK-8 method and flow cytometry were applied to detect A549 cell proliferation and apoptosis, respectively; the Transwell method was applied to detect cell migration and invasion; Western blot was applied to determine the expression of CCL2-CCR2 signaling axis-related proteins. Results: The OD450 value, migration and invasion numbers, and the CCL2, CCR2 protein expression in the low, medium, and high dose phloretin groups were lower than those in the control group (P<0.05), the apoptosis rate of cells was higher (P<0.05), in a dose-dependent manner. The OD450 value, migration and invasion numbers, and the CCL2, CCR2 protein expression in the high dose phloretin+GW0742 group were higher than those in the control group (P<0.05), the apoptosis rate of cells was lower than that in the control group (P<0.05). Conclusion: Phloretin may inhibit the malignant progression of lung cancer cells by regulating the CCL2-CCR2 signaling axis-mediated immune response. 2024 Vol. 30 (7): 1057-1061 [Abstract] ( 96 ) HTML (1 KB)  PDF (1513 KB)  ( 40 ) 1062 Effect of Esketamine on Cognitive Impairment in Depressed Rats via Regulation of the PI3K/Akt/mTOR Signaling Pathway ZHANG Yu, et al DOI: 10.3969/j.issn.1006-6233.2024.07.02 Objective: To investigate the effect of esketamine (ISLAT) on cognitive impairment in rats with depression by regulating the PI3K/Akt/mTOR signaling pathway. Methods: Rats were randomly separated into CK group, Model group, ISLAT low-dose (ISLAT-L) group, ISLAT high-dose (ISLAT-H) group, and ISLAT-H+LY294002 (PI3K pathway inhibitor) group, all rats intraperitoneally injected with the corresponding drug for once a day for 3 weeks. The open field experiment, sugar water preference experiment, and forced swimming experiment were applied to evaluate the depressive behavior and cognitive function of rats. ELISA method was applied to detect the levels of TNF-α, IL-10, and IL-6 in serum, and brain-derived neurotrophic factor (BDNF), norepinephrine (NE), dopamine (DA), and serotonin (5-HT) in hippocampal tissue. HE staining was applied to observe hippocampal tissue damage in rats. TUNEL was applied to detect apoptosis of hippocampal neurons in rats. Western blot was applied to detect the expression of Cleaved-caspase-3, p-PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR proteins in rat hippocampal tissue. Results: Compared with the CK group, the model group showed great damage to the morphology of hippocampal neurons in rats, the number of standing times, sucrose water consumption, serum IL-10 level, BDNF, NE, DA, 5-HT levels in hippocampal tissue, p-PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR protein expression levels were greatly reduced, the swimming immobility time, serum TNF-α and IL-6 levels, neuronal apoptosis rate, and Cleaved-caspase-3 protein expression level were greatly increased (P<0.05). Compared with the Model group, the morphology of hippocampal neurons in the ISLAT-L and ISLAT-H groups showed great improvement, the number of standing times, sucrose water consumption, serum IL-10 level, BDNF, NE, DA, 5-HT levels in hippocampal tissue, p-PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR protein expression levels were greatly increased, the swimming immobility time, serum TNF-α and IL-6 levels, neuronal apoptosis rate, and Cleaved-caspase-3 protein expression level were greatly reduced (P<0.05). LY294002 was able to alleviate the cognitive improvement effect of ISLAT on depression rats (P<0.05). Conclusion: ISLAT can alleviate inflammation, reduce nerve tissue damage and neuronal apoptosis, and improve depression symptoms in rats by activating the PI3K/Akt/mTOR signaling pathway. 2024 Vol. 30 (7): 1062-1067 [Abstract] ( 48 ) HTML (1 KB)  PDF (1748 KB)  ( 36 ) 1068 Effect of LncRNA HCG18 on PD-L1 Expression in Nasopharyngeal Carcinoma Cells and CD8+ T Cell Cytotoxic Activity LIU Chunjiang, et al DOI: 10.3969/j.issn.1006-6233.2024.07.03 Objective: To investigate the regulatory mechanism of LncRNA HCG18 on the function of CD8+ T cells against nasopharyngeal carcinoma (NPC) cells. Methods: The expression of HCG18 in NPC tissues and paracancerous tissues was detected by qRT-PCR. The expression of HCG18 in human nasal mucosal epithelial cells (HNEpC) and NPC cell lines (CNE1, CNE2, HONE-1, HNE-2) was also detected. CNE1 cells were divided into si-HCG18 group, si-NC group, and si-HCG18+PD-L1 group; HNE-2 cells were divided into HCG18 group, Vector group, and HCG18+sh-PD-L1 group. CNE1 and HNE-2 cells in each group were co-cultured with CD8+ T cells for 24 h (si-HCG18+CD8+T group, si-NC+CD8+T group, si-HCG18+PD-L1+CD8+T group, HCG18+CD8+T group, Vector+CD8+T group, and HCG18+sh-PD-L1+CD8+T group). The concentrations of INF-γ, TNF-α, and IL-2 in the co-culture system were detected by enzyme-linked immunosorbent assay (ELISA), and the tumor cell lysis rate was detected by a cytotoxicity assay kit. Western blot was used to detect PD-L1 protein expression. Dual luciferase reporter gene assay was used to verify the targeting relationship between HCG18 and miR-20b-5p, and the targeting relationship between miR-20b-5p and PD-L1. Results: The expression of HCG18 in NPC tissues was significantly higher than that in paracancerous tissues, and the expression of HCG18 in CNE1, CNE2, HONE-1, and HNE-2 cells was significantly higher than that in HNEpC cells (P<0.05). The expression of PD-L1 in CNE1 cells of the si-HCG18 group was significantly lower than that in the si-NC group (P<0.05), and the expression of PD-L1 in HNE-2 cells of the HCG18 group was significantly higher than that in the Vector group (P<0.05). Compared with the si-NC+CD8+T group, the concentrations of INF-γ, TNF-α, and IL-2 in the co-culture system of the si-HCG18+CD8+T group were significantly increased (P<0.05), and the CNE1 cell lysis rate was significantly increased (P<0.05). Compared with the si-HCG18+CD8+T group, the concentrations of INF-γ, TNF-α, and IL-2 in the co-culture system of the si-HCG18+PD-L1+CD8+T group were significantly decreased (P<0.05), and the CNE1 cell lysis rate was significantly decreased (P<0.05). Compared with the Vector+CD8+T group, the concentrations of INF-γ, TNF-α, and IL-2 in the co-culture system of the HCG18+CD8+T group were significantly decreased (P<0.05), and the HNE-2 cell lysis rate was significantly decreased (P<0.05). Compared with the HCG18+CD8+T group, the concentrations of INF-γ, TNF-α, and IL-2 in the co-culture system of the HCG18+sh-PD-L1+CD8+T group were significantly increased (P<0.05), and the HNE-2 cell lysis rate was significantly increased (P<0.05). miR-20b-5p is a target gene of HCG18, and PD-L1 is a target gene of miR-20b-5p. Conclusion: HCG18 upregulates PD-L1 expression in NPC cells and suppresses the anti-tumor activity of CD8+ T cells. 2024 Vol. 30 (7): 1068-1074 [Abstract] ( 42 ) HTML (1 KB)  PDF (1344 KB)  ( 18 ) 1075 LncRNA H19 Targets miR-19b-3p/SOX9 Pathway to Promote Osteogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells FENG Lu, WANG Yao, et al DOI: 10.3969/j.issn.1006-6233.2024.07.04 Objective: To investigate the effects of LncRNA H19 targeting miR-19b-3p/SOX9 pathway on the osteogenic differentiation of adipose-derived mesenchymal stem cells (ADMSCs). Methods: Human ADMSCs were cultured and identified in vitro. The cells were divided into five groups: control group (regular culture), H19 group (transfected with LncRNA H19 lentivirus overexpression vector), LncRNA-NC group (transfected with lentivirus empty vector), miR-19b-3p group (co-transfected with LncRNA H19 lentivirus overexpression vector and miR-19b-3p mimic lentivirus vector), and miR-NC group (co-transfected with LncRNA H19 lentivirus overexpression vector and miR-NC lentivirus vector). The expression of LncRNA H19, miR-19b-3p, SOX9, zinc finger osteoblast-specific transcription factor (Osx), osteocalcin (OCN), and RUNX2 was detected in each group. ALP staining and Alizarin Red staining were observed in each group, cell proliferation activity and ALP activity were compared, and the expression of SOX9, BMP-2, and OPN proteins in each group was analyzed to verify the targeted relationship between LncRNA H19 and miR-19b-3p. Results: Compared with the control group and LncRNA-NC group, the expression of LncRNA H19 and SOX9, Osx, OCN, and RUNX2 mRNA, as well as the expression of SOX9, BMP-2, and OPN proteins, ALP activity, and the amount of mineralized nodule formation were increased in the H19 group, while the expression of miR-19b-3p was decreased (P<0.05). Compared with the H19 group and miR-NC group, the expression of LncRNA H19 and SOX9, Osx, OCN, and RUNX2 mRNA, as well as the expression of SOX9, BMP-2, and OPN proteins, ALP activity, and the amount of mineralized nodule formation were decreased in the miR-19b-3p group, while the expression of miR-19b-3p was increased (P<0.05). There was no statistical difference in the absorbance of each group at 24h, 48h, and 72h (P>0.05). There was a targeted relationship between LncRNA H19 and miR-19b-3p. Conclusion: LncRNA H19 can negatively regulate miR-19b-3p, upregulate SOX9, and contribute to the differentiation of human ADMSC cells into osteoblasts. LncRNA H19 can negatively regulate miR-19b-3p, upregulate SOX9, and promote the osteogenic differentiation of human ADMSCs. 2024 Vol. 30 (7): 1075-1081 [Abstract] ( 38 ) HTML (1 KB)  PDF (2248 KB)  ( 19 ) 1082 Effect of Sufentanil on Lung Injury in Rats with Severe Pneumonia by Regulating TLR4/MyD88/NF-κB Pathway HUANG Fubiao, ZHANG Jingshuo, YE Haibin, et al DOI: 10.3969/j.issn.1006-6233.2024.07.05 Objective: To investigate the effects of sufentanil (Sufen) on lung injury in rats with severe pneumonia (SP) and the Toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-κB (TLR4/MyD88/NF-κB) pathway. Methods: An SP rat model was constructed; the successfully modeled rats were grouped into severe pneumonia group (SP group), low-dose and high-dose sufentanil groups (Sufen-L, Sufen-H groups), and high-dose sufentanil+TLR4 activator LPS group (Sufen-H+LPS group), with 24 rats in each group. An additional 24 normal rats were used as the Control group. The levels of inflammatory factors in alveolar lavage fluid and the number of inflammatory cells in alveolar lavage fluid sediment were detected. The wet to dry weight ratio (W/D) of lung tissue was measured. HE staining was applied to observe the pathological changes in lung tissue of rats in each group. Western blot was applied to detect the expression levels of TLR4, MyD88, p-NF-κB p65/NF-κB p65 in lung tissue. Results: The lung tissue in the SP group was more severely damaged than that in the Control group, with thickening of the lung interstitium, alveolar edema, and extensive infiltration of inflammatory cells, the levels of TNF-α, IL-1β, IL-6, numbers of EOS and NEU inflammatory cells, W/D ratio, the protein expression of TLR4, MyD88, and p-NF-κB p65/NF-κB p65 were greatly increased (P<0.05). Compared with the SP group, the Sufen-L and Sufen-H groups showed reduced lung tissue damage, gradually intact alveolar structure, reduced edema, and reduced infiltration of inflammatory cells, the levels of TNF-α, IL-1β, IL-6, numbers of EOS and NEU inflammatory cells, W/D ratio, the protein expression of TLR4, MyD88, and p-NF-κB p65/NF-κB p65 were greatly reduced, and the Sufen-H group was superior to the Sufen-L group (P<0.05). Compared with the Sufen-H group, the Sufen-H+LPS group had more severe lung tissue damage, severe alveolar structural damage, great alveolar edema, and increased inflammatory cell infiltration in rats, the levels of TNF-α, IL-1β, IL-6, numbers of EOS and NEU inflammatory cells, W/D ratio, the protein expression of TLR4, MyD88, and p-NF-κB p65/NF-κB p65 were greatly increased (P<0.05). Conclusion: Sufen improves lung injury in rats with SP and is associated with inhibition of the TLR4/MyD88/NF-κB signaling pathway. 2024 Vol. 30 (7): 1082-1087 [Abstract] ( 41 ) HTML (1 KB)  PDF (1635 KB)  ( 19 ) 1087 Exploring the Metastatic Mechanism of Gastric Cancer Based on the Ubiquitination Modification of the Protein Kinase NEK9/MTA2 Signaling Pathway WANG Zhiying, et al DOI: 10.3969/j.issn.1006-6233.2024.07.06 Objective: To explore the metastasis mechanism of gastric cancer (GC) based on ubiquitination modification of the never-in-mitosis gene A-related kinase 9 (NEK9)/ metastasis-related tumor gene family 2 (MTA2) signaling pathway. Methods: The Cancer Genome Atlas (TCGA) and Kaplan-Meier Plotter databases were used to analyze the association between NEK9 expression and GC staging and prognosis. In vitro experiments, GC cells were divided into the following groups: control group, shNC group, shNEK9 group, shNC+NC-OE group, shNEK9+NC-OE group, and shNEK9+MTA2-OE group. MTT and Transwell assays were used to determine cell proliferation, migration, and invasion, respectively. Western blot was used to detect the expression of NEK9, MTA2, epithelial-mesenchymal transition (EMT) markers, and PI3K/AKT signaling pathway proteins. Results: TCGA database analysis showed that NEK9 mRNA expression was significantly upregulated in tumor tissues and was closely related to poor prognosis in patients with advanced TNM stage and high NEK9 expression. In addition, NEK9 expression was significantly higher in 7 GC cell lines compared to normal gastric epithelial GES-1 cells (P<0.05). Compared to the control group, shNEK9 group cells showed significantly decreased cell viability, relative colony formation, EdU-positive cell number, invasion, and migration (P<0.05). Moreover, E-cadherin levels were upregulated (P<0.05) and vimentin levels were downregulated (P<0.05) in shNEK9 group cells. Immunoprecipitation assay demonstrated that NEK9 interacted with MTA2. NEK9 knockdown accelerated the degradation of MTA2 in HGC-27 cells, and MTA2 ubiquitination was increased in NEK9-silenced cells. Compared to the shNEK9+NC-OE group, shNEK9+MTA2-OE group cells showed significantly increased relative colony formation, EdU-positive cell number, and migration and invasion (P<0.05). Conclusion: NEK9 is significantly upregulated in GC and its knockdown suppresses GC cell growth and metastasis in vitro. NEK9 may promote tumorigenesis in GC cells by stabilizing MTA2 through deubiquitination and activating the PI3K-AKT signaling pathway. 2024 Vol. 30 (7): 1087-1093 [Abstract] ( 39 ) HTML (1 KB)  PDF (3203 KB)  ( 21 ) 1094 The Mechanism Study of miR-181a Overexpression in Regulating Treg Cells in Children Allergic Rhinitis GAO Xudong, DING Yu, ZHAO Bo, et al DOI: 10.3969/j.issn.1006-6233.2024.07.07 Objective: To investigate the expression of miR-181a in the serum of AR children and to analyze the regulatory effect of miR-181a on the differentiation and function of Treg cells in AR patients. Methods: Totally, 20 children with AR and 20 children of similar age without allergic history were selected as the study subjects. Pearson correlation analysis was used to analyze the relationship between the number of peripheral blood Tregs and miR-181a expression. Tregs were purified from the peripheral blood of AR children. miR-181a mimics and inhibitors were transfected into Tregs cells. The function of Tregs was evaluated by flow cytometry and ELISA. An ovalbumin-induced AR mouse model was established, and then the role of miR-181a in AR was determined by gain-of-function and loss-of-function methods. Results: Compared with the control group, the number of peripheral blood Tregs in AR children was decreased, and the expression of miR-181a was significantly decreased. Pearson correlation analysis showed that the number of peripheral blood Tregs was positively correlated with miR-181a expression (R2=0.335, P<0.001). miR-181a mimics promoted the proliferation of Tregs and up-regulated the mRNA expression of IL-10 and TGF-β. Luciferase reporter assay showed that miR-181a directly targeted the 3'UTR of OPN. In animal experiments, compared with the AR and AR+miR-NC groups, the AR+miR-181a mimic group had less inflammation, and the AR+miR-181a inhibitor group had more severe inflammation. Recombinant OPN protein significantly reversed the anti-inflammatory effect of miR-181a mimics. In addition, the number of Treg cells in AR mice was significantly decreased, miR-181a mimics significantly increased the number of Treg cells in AR mice, and recombinant OPN protein significantly reversed the increase of Treg cells induced by miR-181a mimics. Conclusion: The up-regulation of miR-181a significantly reduced the expression of OPN, thereby reducing eosinophils and enhancing Treg function, alleviating the occurrence of airway inflammation during the development of AR. 2024 Vol. 30 (7): 1094-1100 [Abstract] ( 30 ) HTML (1 KB)  PDF (1655 KB)  ( 13 ) 1100 Effect of Oridonin on Apoptosis of Osteoblasts in Osteoporotic Rats by Regulating Hippo/YAP Signaling Pathway LU Haifeng, ZOU Binghong, ZHU Yi DOI: 10.3969/j.issn.1006-6233.2024.07.08 Objective: To investigate the effect of oridonin (Ori) on osteoblast apoptosis in osteoporosis (OP) rats by regulating the Hippo/Yes-associated protein (YAP) signaling pathway. Methods: Ten rats were randomly selected as the sham surgery group, with only ovaries separated but not removed. The remaining rats were used to establish an OP model by removing both ovaries. The successfully modeled rats were randomly separated into the OP group, different doses of Ori (5 mg/kg Ori, 10 mg/kg Ori, 20 mg/kg Ori) groups, and 20 mg/kg Ori+Hippo/YAP activator - Vesteporfin (10 mg/kg) group. After the intervention, abdominal aortic blood was collected, and ELISA was applied to detect serum levels of osteocalcin (BGP) and alkaline phosphatase (ALP). The femoral tissue was separated, and a dual-energy X-ray bone density instrument was applied to detect femoral mineral content and bone density. HE was applied to detect morphological changes in femoral tissue. TUNEL was applied to detect changes in osteoblast apoptosis. Western blot was applied to detect the expression of YAP and transcription co-activator PDZ binding motif (TAZ) proteins. Results: There was no great change in the femoral structure of the rats in the sham surgery group. Compared with the sham surgery group, there was a great change in the morphology of the femoral tissue in the OP group, the levels of BGP and ALP, femoral tissue mineral content, bone density, YAP, and TAZ protein expression were greatly reduced (P<0.05), the apoptosis rate of osteoblasts was greatly increased (P<0.05). Compared with the OP group, the femoral tissue morphology was improved in the 5 mg/kg Ori group, 10 mg/kg Ori group, and 20 mg/kg Ori group, the levels of BGP and ALP, femoral tissue mineral content, bone density, YAP, and TAZ protein expression were greatly increased (P<0.05), the apoptosis rate of osteoblasts was greatly decreased (P<0.05), there were statistical differences between different doses of Ori (P<0.05). Compared with the 20 mg/kg Ori group, the morphological changes of the femoral tissue in the 20 mg/kg Ori+Verteporfin group were aggravated, the levels of BGP and ALP, femoral tissue mineral content, bone density, YAP, and TAZ protein expression were greatly reduced (P<0.05), the apoptosis rate of osteoblasts was greatly increased (P<0.05). Conclusion: Ori reduces osteoblast apoptosis and alleviates symptoms in OP rats by regulating the Hippo/YAP signaling pathway. 2024 Vol. 30 (7): 1100-1105 [Abstract] ( 31 ) HTML (1 KB)  PDF (1925 KB)  ( 14 ) 1105 The Effect of Eriodictyol on Osteogenic Differentiation of Periodontitis Periodontal Ligament Stem Cells by Regulating the YAP/TAZ Signaling Pathway ZHAO Yanhuan, et al DOI: 10.3969/j.issn.1006-6233.2024.07.09 Objective: To investigate the effect of eriodictyol on the osteogenic differentiation of human periodontal ligament stem cells (hPDLSC) induced by lipopolysaccharide (LPS) by regulating the Yes-associated protein (YAP)/transcriptional co-activator (TAZ) signaling pathway. Methods: The Periodontitis model of rats was established by the ligation method. After successful modeling, the rats were randomly divided into the model group and the eriodictyol group, with 6 rats in each group, and 6 normal rats as the control group. HPDLSC was cultured in vitro, induced with 10 μg/mL LPS, and treated with 0, 40, 80, 160, 320, and 480 μmol/L of eriodictyol, the alkaline phosphatase (ALP) activity of cells in each treatment group was detected with a kit to screen the optimal concentration of eriodictyol. hPDLSC were randomly divided into control group, LPS group, LPS+eriodictyol group, LPS+empty group, LPS+eriodictyol+YAP knockdown group, after inducing osteogenic differentiation and treated with LPS, eriodictyol, and plasmids. The pathological changes of periodontal tissue were observed by HE staining. Real-time fluorescence quantitative PCR and immunoblotting experiments were applied to detect the expression of YAP and TAZ in periodontal tissues and cells. ALP activity detection kit, ALP staining, and alizarin red staining were applied to detect osteogenic differentiation of cells. Enzyme linked immunosorbent assay (ELISA) was applied to detect the levels of inflammatory cytokines prostaglandin E2 (PGE2), interleukin (IL)-6 and IL-18 in periodontal tissues and cells. And real-time fluorescent quantitative PCR and Western blot were applied to detect the expression of osteogenic differentiation factors Runx2, OSX, and osteopontin (OPN) of cells. Results: Compared with the control group, the periodontal tissue of the model group showed obvious pathological damage, the serum levels of PGE2, IL-6 and IL-18 were increased, and the mRNA and protein expressions of YAP and TAZ were decreased (P<0.05). Compared with the model group, the pathological damage of periodontal tissue in the eriodictyol group was alleviated, the levels of serum PGE2, IL-6, and IL-18 were decreased, and the mRNA and protein expressions of YAP and TAZ in periodontal tissue were increased (P<0.05). The ALP activity in hPDLSC induced by LPS was increased after treatment with 40, 80, 160, 320, and 480 μmol/L eriodictyol (P<0.05), and the increasing effect was enhanced with the increase of the concentration of eriodictyol and reached a plateau at 320 μmol/L. Therefore, 320μmol/L sericol was selected for subsequent experiments. Compared with the control group, the expression of YAP and TAZ mRNA and protein, the relative proportion of ALP positivity, ALP activity, the relative proportion of mineralized nodules, the expression of Runx2 and OSX, OPN mRNA and protein in the LPS group decreased (P<0.05), the levels of PGE2, IL-6, and IL-18 increased (P<0.05). Compared with the LPS group, the expression of YAP and TAZ mRNA and protein, the relative proportion of ALP positivity, ALP activity, the relative proportion of mineralized nodules, the expression of Runx2 and OSX, OPN mRNA and protein in LPS+eriodictyol group increased (P<0.05), the levels of PGE2, IL-6, and IL-18 decreased (P<0.05); there was no obvious change in all indicators of cells in the LPS+empty group (P>0.05). Compared with the LPS+eriodictyol group, the expression of YAP and TAZ mRNA and protein, the relative proportion of ALP positivity, ALP activity, the relative proportion of mineralized nodules, the expression of Runx2 and OSX, OPN mRNA and protein in LPS+eriodictyol+YAP knockdown group decreased (P<0.05), the levels of PGE2, IL-6, and IL-18 increased (P<0.05). Conclusion: Eriodictyol can reduce the inflammatory damage of periodontitis rats, and inhibit LPS-induced hPDLSC inflammation by up-regulating the expression of YAP/TAZ pathway proteins, thereby promoting its osteogenic differentiation. 2024 Vol. 30 (7): 1105-1114 [Abstract] ( 36 ) HTML (1 KB)  PDF (1884 KB)  ( 19 ) 1114 The Role and Significance of Blood Lactate and FGF21 in the Diagnosis and Efficacy Monitoring of Mitochondrial Myopathy in Children WANG Zuohua, WANG Huiping, WANG Chunxia, et al DOI: 10.3969/j.issn.1006-6233.2024.05.010 Objective: To analyze the role and significance of blood lactate and fibroblast growth factor 21 (FGF21) in the diagnosis and efficacy monitoring of mitochondrial myopathy (ME) in children. Methods: A total of 141 subjects were enrolled in this study, including 47 children with ME (ME group), 47 children with non-mitochondrial myopathies (muscle disease group), and 47 healthy children (control group), all of whom were admitted to Kunming Children's Hospital from August 2020 to February 2023. The ME group received dietary and exercise guidance, lamotrigine, idebenone, vitamin C, folic acid, and coenzyme Q10 for treatment. The efficacy was evaluated 3 months after treatment, and the ME patients were divided into controlled and uncontrolled subgroups according to the efficacy. Fasting blood samples were collected before treatment, 1 month after treatment, and 3 months after treatment. Blood lactate levels were measured using the American YSI 1500 SPORT blood lactate analyzer and matching reagent kit, and serum FGF21 levels were measured using enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunosorbent assay (ELISA). The levels of blood lactate and FGF21 in the three groups before treatment were compared. The interaction coefficient γ and OR values were used to analyze the interaction of blood lactate and FGF21 on the susceptibility of children to ME. The receiver operating characteristic (ROC) curve was used to analyze the value of blood lactate and FGF21 in the diagnosis of ME in children. The changes in blood lactate, FGF21, and Newcastle Mitochondrial Disease Assessment Scale (NMDAS) scores before and after treatment were compared between ME patients with different efficacies. Pearson correlation analysis was used to analyze the correlation between the decrease in blood lactate and FGF21 after 1 month and 3 months of treatment and the decrease in NMDAS score. Results: Compared with the control group, the levels of blood lactate and FGF21 were elevated in the ME group (t=13.901, 38.082, P<0.05); compared with the muscle disease group, the levels of blood lactate and FGF21 were also elevated in the ME group (t=12.734, 35.696, P<0.05); there was no significant difference in blood lactate and FGF21 levels between the control group and the muscle disease group (t=0.821, 1.326, both P>0.05); interaction analysis showed that the OR value caused by the coexistence of blood lactate and FGF21 was 1350.500, the regression coefficient was 7.208, the interaction OR value was less than the product of the OR values of the two single factors, which was a sub-multiplicative model, γ=1.535>1, and the effect of blood lactate on FGF21 had a positive interaction effect; ROC curve showed that the AUC of blood lactate + FGF21 for the diagnosis of ME in children was 0.904, which was higher than that of blood lactate (0.839) and FGF21 (0.719), with a diagnostic sensitivity of 78.72% and a specificity of 87.23%; the levels of blood lactate, FGF21 and NMDAS scores in patients with controlled disease were lower than those in patients with uncontrolled disease after 1 month and 3 months of treatment (P<0.05); correlation analysis showed that the decrease in blood lactate and FGF21 after 1 month and 3 months of treatment was positively correlated with the decrease in NMDAS score after 1 month and 3 months of treatment (P<0.05). Conclusion: The blood lactate and FGF21 levels in children with ME are elevated, and there is a positive interaction between the two, which is closely related to the condition and efficacy of the disease. 2024 Vol. 30 (7): 1114-1120 [Abstract] ( 19 ) HTML (1 KB)  PDF (1621 KB)  ( 14 ) 1120 Clinical Efficacy of Compound Betamethasone Combined with Iliac Fascia Block in Postoperative Analgesia for Patients Undergoing Total Hip Arthroplasty: Effects on TLR4/NF-κB Signaling Pathway WANG Kexin, LIU Zhong, WANG Mengnan, et al DOI: 10.3969/j.issn.1006-6233.2024.07.011 Objective: To explore the clinical value of compound betamethasone combined with iliac fascia block in postoperative analgesia for patients undergoing total hip arthroplasty (THA), and its influence on the TLR4/NF-κB signaling pathway. Methods: A total of 152 patients undergoing THA from November 2022 to December 2023 were included in this study. Patients were randomly divided into two groups: the control group received iliac fascia block, and the experimental group received compound betamethasone and iliac fascia block. Parameters compared between the two groups included perioperative indicators, pain scores, hip joint mobility, TLR4/NF-κB signaling pathway factors, and incidence of adverse reactions. Results: There were no significant differences between two groups in terms of operation time, blood loss, anesthesia time, time to first ambulation, and intraoperative fluid infusion (P>0.05). Pain scores at 24 hours and 48 hours postoperatively were significantly lower in the experimental group than in the control group (P<0.05), while there was no significant difference in pain scores at 2 hours postoperatively between the two groups (P>0.05). At 7 days and 14 days postoperatively, hip joint mobility was significantly higher in the experimental group compared to the control group, and at 14 days postoperatively, hip joint mobility improved significantly in both groups (P<0.05). Serum levels of TLR4 and NF-κB at 24 hours and 48 hours postoperatively were significantly lower in the experimental group than in the control group (P<0.05), while there was no significant difference in serum levels of TLR4 and NF-κB before surgery between the two groups (P>0.05). During treatment, neither group experienced adverse reactions such as nausea, vomiting, or itching. Conclusion: Compound betamethasone combined with iliac fascia block effectively reduces pain intensity, improves hip joint mobility, and is associated with the expression of TLR4/NF-κB signaling pathway in patients undergoing THA, demonstrating safety and efficacy. 2024 Vol. 30 (7): 1120-1125 [Abstract] ( 28 ) HTML (1 KB)  PDF (1155 KB)  ( 13 ) 1125 Comparative Observation of MRI-SWI T1WI T2WI and T2FLAIR in Diagnosing Intracranial Cavernous Hemangiomas FENG Tianbao, XU Chao, et al DOI: 10.3969/j.issn.1006-6233.2024.07.012 Objective: To investigate the diagnostic value of magnetic resonance susceptibility-weighted imaging (MRI-SWI), T1-weighted imaging (T1WI), T2-weighted imaging (T2WI), and T2 fluid-attenuated inversion recovery (T2FLAIR) in intracranial cavernous hemangioma. Methods: From March 2019 to March 2023, 153 patients suspected of intracranial cavernous hemangioma were selected. All patients underwent preoperative MRI examinations, including MRI-SWI, T1WI, T2WI, and T2FLAIR. Surgical pathology results were used as the gold standard. The diagnostic values of the different MRI sequences were calculated. Results: MRI-SWI correctly diagnosed 97 patients with a total of 177 lesions, whereas T1WI correctly diagnosed 72 patients with 121 lesions, T2WI correctly diagnosed 77 patients with 132 lesions, and T2FLAIR correctly diagnosed 82 patients with 143 lesions. The detection rate of MRI-SWI for cavernous hemangiomas was significantly higher than that of T1WI, T2WI, and T2FLAIR (χ2=28.698, P<0.05; χ2=22.299, P<0.05; χ2=16.257, P<0.05). The detection rate of T2FLAIR was higher than that of T1WI (χ2=7.211, P<0.05). There were no significant differences in detection rates between T1WI and T2WI or between T2FLAIR and T2WI (χ2=1.676, P>0.05; χ2=1.972, P>0.05). MRI signal characteristics of cavernous hemangiomas varied among the sequences. T1WI mainly showed mixed signals (43.80%) and low signals (30.58%). T2WI mainly showed mixed signals (49.24%), characterized by central punctate or reticulated high signals with low-signal peripheral borders, known as the "iron ring sign," followed by low signals (35.61%). T2FLAIR mainly showed round or oval mixed signals (58.33%) with an internal appearance resembling popcorn or reticulation, followed by low signals (34.09%). MRI-SWI predominantly showed low signals (95.48%), highlighting iron deposition areas and tumor bodies with patchy or punctate low signals in and around the lesions. Sensitivity and specificity for diagnosing cavernous hemangiomas were as follows: T1WI had a sensitivity of 74.23% and specificity of 85.71%; T2WI had a sensitivity of 79.38% and specificity of 83.93%; T2FLAIR had a sensitivity of 84.54% and specificity of 78.57%; MRI-SWI had a sensitivity of 100% and specificity of 94.64%. Conclusion: MRI-SWI provides a higher diagnostic value for intracranial cavernous hemangiomas compared to T1WI, T2WI, and T2FLAIR sequences. 2024 Vol. 30 (7): 1125-1130 [Abstract] ( 28 ) HTML (1 KB)  PDF (1677 KB)  ( 10 ) 1130 Analysis of Lipid Profile and Homocysteine Levels in Subclinical Hypothyroidism Patients and Their Relationship with TSH MA Wangwen, ZHAO Yanping, LI Wenya DOI: 10.3969/j.issn.1006-6233.2024.07.013 Objective: To analyze the levels of blood lipids and homocysteine (Hcy) in patients with subclinical hypothyroidism (SCH) and their relationship with thyroid-stimulating hormone (TSH). Methods: A total of 119 patients diagnosed with SCH between March 2020 and March 2023 were selected as the SCH group. Additionally, 121 healthy individuals with normal health examination indicators during the same period were selected as the normal control group. Blood lipid metabolism, Hcy, and TSH levels were compared between the SCH group and the normal control group. Within the SCH group, subgroup analysis (SCH1 and SCH2) based on TSH levels was conducted to compare lipid metabolism and Hcy levels. Correlation analysis using partial correlation coefficients and multiple linear regression were performed to assess the association between blood lipid metabolism, Hcy levels, and TSH levels. Results: Compared with the normal control group, the SCH group showed higher levels of total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), Hcy, and TSH (P<0.05). There were no statistically significant differences in high-density lipoprotein cholesterol (HDL-C), free triiodothyronine (FT3), and free thyroxine (FT4) levels between the SCH group and the normal control group (P>0.05). Subgroup analysis within the SCH group revealed that TC, LDL-C, and Hcy levels were higher in SCH2 compared to SCH1 (P<0.05). There were no significant differences in TG, HDL-C, FT3, and FT4 levels between SCH1 and SCH2 (P>0.05). After adjusting for gender, age, BMI, smoking history, fasting plasma glucose (FPG), and TSH, multiple linear regression analysis indicated that TSH was a risk factor for abnormalities in TC, TG, LDL-C, and Hcy levels, while the linear regression relationship between TSH and HDL-C was not statistically significant(P>0.05). Conclusion: Patients with subclinical hypothyroidism exhibit alterations in blood lipid metabolism and Hcy levels, which are influenced by TSH levels. Therefore, monitoring blood lipid metabolism and Hcy levels in patients with subclinical hypothyroidism should be strengthened. This study provides a new perspective for clinicians to assess and manage cardiovascular risks in patients with subclinical hypothyroidism. 2024 Vol. 30 (7): 1130-1134 [Abstract] ( 34 ) HTML (1 KB)  PDF (1139 KB)  ( 21 ) 1134 Predictive Value of Uric Acid Albumin Ratio for Coronary Slow Flow Phenomenon HANIKEZI kasimu, MIHAILI Maranmu, MA Yitong DOI: 10.3969/j.issn.1006-6233.2024.07.014 Objective: To investigate the correlation between uric acid albumin ratio (UAR) and coronary slow flow (CSF) and evaluate the predictive value of UAR for CSF. Methods: A retrospective analysis was conducted on 610 patients with chest pain who underwent coronary angiography at the Heart Center of Xinjiang Medical University. Electronic medical records and coronary angiography results were collected, and patients were grouped according to the presence or absence of CSF. Multivariable-adjusted methods were used to assess the correlation between uric acid (UA), albumin (ALB), and UAR and the occurrence of CSF. ROC curves and RCS curves were used to evaluate the relationship between the above indicators and CSF risk. Results: Compared with the control group, the CSF group had a higher proportion of males and higher rates of smoking and alcohol consumption. The CSF group had higher body mass index (BMI), hemoglobin (Hb), aspartate aminotransferase (AST), UA, and UAR levels, and lower ALB levels (P<0.05).Multivariable-adjusted logistic regression analysis showed that high UA levels (OR: 1.010, 95%CI: 1.007-1.013, P<0.001), UAR (OR: 1.513, 95%CI: 1.341-1.707, P<0.001), and low ALB levels (OR: 0.794, 95%CI: 0.733-0.860, P<0.001) were independent risk factors for CSF.ROC curve analysis showed that UA, ALB, and UAR all had good predictive value for CSF, among which UAR had the best performance (P<0.05).RCS curve analysis showed that UAR and CSF risk were generally positively correlated. Conclusion: The results suggest that UAR is associated with CSF and may be a useful biomarker for predicting CSF. 2024 Vol. 30 (7): 1134-1139 [Abstract] ( 39 ) HTML (1 KB)  PDF (1425 KB)  ( 25 ) 1140 Effect of IPS e.max Press Heat-Pressed Cast Porcelain Full Crowns and Zirconia All-Ceramic Crowns on Gingival Health and Aesthetics in Anterior Tooth Restoration Patients LIU Xiaoyu, CHEN Xiwen, ZHUANG Mengyao, et al DOI: 10.3969/j.issn.1006-6233.2024.07.015 Objective: To compare the effects of IPS e.max press heat-pressed cast porcelain full crowns and zirconia all-ceramic crowns on gingival health and aesthetics in anterior tooth restoration patients, and to provide clinical reference for the selection of materials for anterior tooth aesthetic restoration. Methods: A total of 104 patients who underwent anterior tooth restoration in our hospital from May 2021 to May 2022 were selected as the study subjects and were randomly divided into two groups of 52 patients each using a random number table. The observation group was restored with IPS e.max press cast porcelain full crowns, and the control group was restored with zirconia all-ceramic crowns. The clinical restoration effects and post-restoration gingival index, plaque index, gingival sulcus bleeding index, and gingival sulcus inflammatory factor levels of the two groups were compared. Results: The marginal adaptation and secondary caries of the observation group were superior to those of the control group (P<0.05); after restoration, the gingival index, plaque index, etc. of the observation group were significantly lower than those of the control group, and the levels of IL-6, IL-8, and TNF-α in the gingival sulcus fluid were significantly lower than those of the control group (P<0.05); 12 months after restoration, the PES score of the observation group was higher than that of the control group (P<0.05). Conclusion: Compared with zirconia all-ceramic crowns, IPS e.max press cast porcelain full crowns have good anterior tooth aesthetic restoration effect, can reduce the level of inflammatory factors in the gingival sulcus, improve gingival health, and can also improve the aesthetics of anterior teeth. 2024 Vol. 30 (7): 1140-1145 [Abstract] ( 26 ) HTML (1 KB)  PDF (1524 KB)  ( 17 ) 1145 Efficacy of Laparoscopic Transumbilical Single-Site Appendectomy for Pediatric Appendicitis and Its Impact on Inflammatory Factors WEI Biao, CHEN Shan, CHEN Xin, et al DOI: 10.3969/j.issn.1006-6233.2024.07.016 Objective: To investigate the efficacy of laparoscopic transumbilical single-site appendectomy (LTSSA) for pediatric appendicitis and its impact on inflammatory factors. Methods: The clinical data of pediatric appendicitis patients treated in our hospital from March 2022 to June 2023 were retrospectively analyzed. 55 patients who underwent LTSSA were randomly selected from the LTSSA group using a random number table, and 50 patients who underwent traditional laparoscopic appendectomy (LTA) were randomly selected from the LTA group using a random number table. The surgical parameters, postoperative recovery, and postoperative complications of the two groups were compared. The improvement of appendicitis symptoms before and 5 days after surgery [Appendicitis Inflammatory Response (AIR) score, Visual Analogue Scale (VAS) score] and the levels of inflammatory factors before, 48 hours after surgery and 5 days after surgery were compared. Results: The operation time, intraoperative bleeding volume, time of first postoperative flatus, time of normal body temperature recovery, time of getting out of bed and activity, length of hospital stay, and incidence of postoperative complications were all lower in the observation group than in the control group, with statistically significant differences (P<0.05). At 5 days after surgery, the AIR and VAS scores of the two groups were lower than before surgery, and the decrease in the observation group was greater than that in the control group, with statistically significant differences (P<0.05). Repeated measures ANOVA showed that the time point effect, group effect, time point and group interaction effect of the two groups of patients after surgery can all affect the changes in CRP, PCT, and WBC levels (P<0.05), and the observation group was lower than the control group, with statistically significant differences (P<0.05). Conclusion: LTSSA for pediatric appendicitis has a positive effect, reduces postoperative complications, lowers inflammatory factor levels, and helps in the rapid recovery of patients. 2024 Vol. 30 (7): 1145-1150 [Abstract] ( 33 ) HTML (1 KB)  PDF (1150 KB)  ( 11 ) 1150 Characterization Analysis of High-Frequency Ultrasound and Different Parameter Elasticity Imaging of Invasive Ductal Breast Cancer and Its Diagnosis of Lymph Node Metastasis YUAN Min, YAO Xiaojun, XIE Shuhui, et al DOI: 10.3969/j.issn.1006-6233.2024.07.017 Objective: To investigate the characteristics of high-frequency ultrasound and different parameter elasticity imaging of invasive ductal breast cancer, and to analyze its diagnostic value for lymph node metastasis. Methods: A retrospective study was conducted on 96 patients with invasive ductal breast cancer who were diagnosed by pathology and treated in our hospital from January 2021 to January 2023. According to the lymph node metastasis, the patients were divided into metastasis group and non-metastasis group. All patients underwent high-frequency ultrasound and ultrasound elastography examination upon admission. The high-frequency ultrasound and ultrasound elastography parameters of patients with different lymph node metastasis were compared. Logistic regression analysis was used to analyze the relationship between these parameters and lymph node metastasis in patients with invasive ductal breast cancer. ROC curves were drawn to explore the diagnostic value of high-frequency ultrasound and ultrasound elastography parameters for lymph node metastasis. Results: Among the 96 patients with invasive ductal breast cancer, 59 cases (61.46%) had lymph node metastasis and 37 cases (38.54%) had no lymph node metastasis. The cortical thickness of axillary lymph nodes, the longitudinal diameter of axillary lymph nodes, and the longitudinal-to-transverse diameter ratio in the metastasis group were greater than those in the non-metastasis group. The proportion of peripheral or mixed blood flow patterns and the proportion of lymph node disappearance were higher in the metastasis group than in the non-metastasis group (P<0.05). The maximum elasticity modulus value, elasticity data dispersion value, and the ratio of lesion to surrounding tissue in the metastasis group were greater than those in the non-metastasis group (P<0.05). Logistic regression analysis showed that the risk factors for lymph node metastasis in patients with invasive ductal breast cancer were large axillary lymph node cortical thickness, large axillary lymph node longitudinal diameter, large longitudinal-to-transverse diameter ratio, peripheral or mixed blood flow pattern, lymph node disappearance, large maximum elasticity modulus value, large elasticity data dispersion value, and large ratio of lesion to surrounding tissue (OR>1, P<0.05). ROC curve analysis showed that axillary lymph node cortical thickness, axillary lymph node longitudinal diameter, longitudinal-to-transverse diameter ratio, blood flow pattern, lymph node disappearance, maximum elasticity modulus value, elasticity data dispersion value, and the ratio of lesion to surrounding tissue had a certain predictive value for lymph node metastasis in patients with invasive ductal breast cancer (AUC=0.833, 0.782, 0.758, 0.721, 0.646, 0.714, 0.685, 0.730), and the combined diagnostic value was higher (AUC=0.908). Conclusion: The characteristics of high-frequency ultrasound and ultrasound elastography in patients with invasive ductal breast cancer are closely related to lymph node metastasis and can be used for preoperative diagnosis of lymph node metastasis. 2024 Vol. 30 (7): 1150-1156 [Abstract] ( 27 ) HTML (1 KB)  PDF (1467 KB)  ( 14 ) 1156 Expression Levels of miR-155 and miR-132 in Peripheral Blood of Patients with Diabetic Foot Infection and Their Correlation with the Degree of Lower Limb Vascular Lesions ZHANG Lin, DONG Peng, WANG Ning, et al DOI: 10.3969/j.issn.1006-6233.2024.07.018 Objective: To explore the expression levels of peripheral blood miR-155 and miR-132 in patients with diabetic foot (DF) infection and the relationship with lower extremity vascular lesion degree. Methods: A total of 62 patients with simple type 2 diabetes mellitus (T2DM) in the hospital from June 2020 to June 2023 were selected and included in T2DM group, and 93 patients with T2DM complicated with DF infection during the same period were enrolled as DF infection group. The levels of peripheral blood miR-155 and miR-132 were compared, and the relationship between levels of peripheral blood miR-155 and miR-132 and clinical characteristics in patients with DF infection was analyzed. According to the classification criteria of the Transatlantic Association of Society Consensus (TASC), patients with DF infection were classified into grades A, B, C, and D. The levels of miR-155 and miR-132 in peripheral blood were compared among patients with DF infection and different lower extremity vascular lesion degrees. The Spearman method was used to analyze the correlation between peripheral blood miR-155 and miR-132 and lower extremity vascular lesion degree. Results: The level of miR-155 in DF infection group was higher than that in T2DM group while the level of miR-132 was lower than that in T2DM group (all P<0.05). The course of DF ulcer, wound area, and proportion of Wagner grade IV in the high miR-155 expression group were higher than those in the low expression group, while the course of DF ulcer, wound area, and proportion of Wagner grade IV were lower in high miR-132 expression group than those in low expression group, and the healing rate of DF ulcer after 8 weeks was higher than that in low expression group (all P<0.05). The miR-155 level in patients with DF infection of grade A to D was increased in turn while the miR-132 level was decreased in turn (all P<0.05). Spearman analysis showed that the expression level of miR-155 was positively correlated with lower extremity vascular lesion degree, while the expression level of miR-132 was negatively correlated with lower extremity vascular degree (all P<0.05). Conclusion: The high expression of miR-155 in the peripheral blood of patients with DF infection is positively correlated with degree of lower extremity vascular lesion, and the low expression of miR-132 is negatively correlated with the degree of lower extremity vascular lesion. 2024 Vol. 30 (7): 1156-1160 [Abstract] ( 39 ) HTML (1 KB)  PDF (1138 KB)  ( 24 ) 1160 Combined Detection of High-Mobility Group Box 1 Protein Macrophage Migration Inhibitory Factor (MIF) and CXC Chemokine Subfamily 16 for the Diagnosis of High-Risk Human Papillomavirus Infection CAI Rongrong, SHI Pingping, GU Chunyan DOI: 10.3969/j.issn.1006-6233.2024.07.019 Objective: To investigate the expression levels and diagnostic value of high-mobility group box 1 protein (HMGB1), macrophage migration inhibitory factor (MIF), and CXC chemokine subfamily 16 (CXCL16) in patients with high-risk human papillomavirus (HPV) infection. Methods: A total of 150 patients with HPV infection admitted to our hospital from October 2020 to October 2023 were enrolled in the study group and divided into high-risk HPV infection group (n=40) and low-risk HPV infection group (n=110) according to the results of nucleic acid detection. Another 100 healthy women who underwent transvaginal colposcopy in our hospital during the same period were selected as the control group. The HPV-DNA genotyping kit was used to detect HPV in the study group patients; enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of HMGB1 and CXCL16 in the study group and control group; and immunohistochemistry was used to detect the staining intensity of MIF. The differences in HMGB1, MIF, and CXCL16 between the control group and the study group were compared; the differences in HMGB1, MIF, and CXCL16 between the low-risk HPV infection group and the high-risk HPV infection group were compared; receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic value of HMGB1, MIF, and CXCL16 combined detection for high-risk HPV infection; Spearman correlation analysis was used to analyze the correlation between HMGB1, MIF, and CXCL16 and different HPV infection genotypes. Results: Compared with the control group, the levels of serum HMGB1 and CXCL16, and the score of MIF positive cell proportion in the study group were significantly higher (all P<0.05); compared with the low-risk HPV infection group, the levels of serum HMGB1 and CXCL16, and the score of MIF positive cell proportion in the high-risk HPV infection group were significantly higher (all P<0.05); ROC curve analysis showed that the AUC of HMGB1, MIF, and CXCL16 for the diagnosis of high-risk HPV infection alone and in combination were 0.748, 0.684, 0.791, and 0.934, respectively. The diagnostic value of combined detection was significantly higher than that of single detection (Z=2.577, 3.152, 2.096, all P<0.05); Spearman correlation analysis showed that HMGB1, MIF, and CXCL16 were significantly positively correlated with different HPV infection genotypes (r=0.615, 0.633, 0.649, all P<0.05). Conclusion: The levels of serum HMGB1 and CXCL16, and the score of MIF positive cell proportion in patients with high-risk HPV infection were significantly higher than those in the low-risk and control groups. The combined detection of HMGB1, MIF, and CXCL16 in clinical examination can improve the clinical diagnosis rate of high-risk HPV infection and provide a new detection method and treatment basis for clinical practice. 2024 Vol. 30 (7): 1160-1165 [Abstract] ( 29 ) HTML (1 KB)  PDF (1369 KB)  ( 11 ) 1165 Expression of STAT3 and ADAMTS19 in Endometrial Cancer Tissues and Their Relationship with Pathological Parameters and Prognosis GAO Jun, ZHAO Xiaojuan, JIN Xinxing DOI: 10.3969/j.issn.1006-6233.2024.07.020 Objective: To investigate the expression of signal transducer and activator of transcription 3 (STAT3) and A disintegrin and metalloproteinase with thrombospondin motifs 19 (ADAMTS19) in endometrial carcinoma (EC) tissues and the relationship with pathological parameters and prognosis. Methods: A total of 82 patients with EC who underwent surgical resection in our hospital from January 2018 to December 2020 were selected. Immunohistochemistry was used to detect the expression of STAT3 and ADAMTS19 in EC tissues and paracancerous tissues. The correlation between STAT3 and ADAMTS19 expression in EC tissues was analyzed using Phi correlation coefficient. According to the expression of STAT3 and ADAMTS19 in EC tissues, the patients were divided into STAT3 and ADAMTS19 positive/negative expression groups. Kaplan-Meier method was used to draw the survival curves of EC patients with STAT3 and ADAMTS19 positive/negative expression, and Cox regression analysis was used to analyze the factors affecting the prognosis of EC patients; ROC curve was used to evaluate the predictive value of STAT3 and ADAMTS19 expression in cancer tissues for the death of EC patients. Results: Compared with paracancerous tissues, the positive expression rate of STAT3 in EC tissues was increased, and the positive expression rate of ADAMTS19 was decreased (P<0.05). The expression of STAT3 and ADAMTS19 in EC tissues was negatively correlated (φ=-0.443, P<0.001). The expression of STAT3 and ADAMTS19 was related to the International Federation of Gynecology and Obstetrics (FIGO) stage, depth of myometrial invasion, degree of differentiation and lymph node metastasis of EC patients (P<0.05). The 3-year overall survival rate of the 82 patients with EC was 69.51% (57/82). The 3-year overall survival rate of the STAT3 positive expression group was 60.71%, which was lower than that of the STAT3 negative expression group (88.46%), and the 3-year overall survival rate of the ADAMTS19 positive expression group was 88.89%, which was higher than that of the ADAMTS19 negative expression group (64.06%) (P<0.05). FIGO stage Ⅲ, myometrial invasion depth ≥1/2, low differentiation, lymph node metastasis and STAT3 positive expression were independent risk factors for the death of EC patients, and ADAMTS19 positive expression was an independent protective factor (P<0.05). The area under the curve (AUC) of the joint prediction of STAT3 and ADAMTS19 expression in cancer tissues for the death of EC patients was 0.860, which was greater than that of the separate prediction of STAT3 and ADAMTS19 expression in cancer tissues (0.758, 0.750) (P<0.05). Conclusion: STAT3 was highly expressed and ADAMTS19 was lowly expressed in EC tissues, and their expressions were negatively correlated. They were significantly related to FIGO stage, depth of myometrial invasion, degree of differentiation, lymph node metastasis and prognosis. The joint expression of STAT3 and ADAMTS19 had a high predictive value for the prognosis of EC patients. 2024 Vol. 30 (7): 1165-1171 [Abstract] ( 25 ) HTML (1 KB)  PDF (1475 KB)  ( 15 ) 1171 Predictive Value of Magnetic Resonance Diffusion-Weighted Imaging Apparent Diffusion Coefficient Combined with Serum Tumor Markers for Postoperative Recurrence and Metastasis in Patients with Colorectal Cancer YAO Jun, et al DOI: 10.3969/j.issn.1006-6233.2024.07.021 Objective: To investigate the predictive value of magnetic resonance diffusion-weighted imaging (MRI-DWI) apparent diffusion coefficient (ADC) combined with serum tumor markers [carcinoembryonic antigen (CEA), carbohydrate antigen 199 (CA199), carbohydrate antigen 724 (CA724)] for postoperative recurrence and metastasis in patients with colorectal cancer. Methods: Eighty patients with colorectal cancer who underwent radical resection in our hospital from January 2020 to January 2022 were enrolled in the case group. Another 80 healthy subjects who underwent colorectal cancer screening and were excluded from colorectal cancer in our hospital during the same period were enrolled in the control group. Both groups underwent MRI-DWI and serum examination, and the ADC values and serum tumor marker levels of the two groups were compared. The patients in the case group were followed up after surgery, and the patients were divided into the recurrence group and the non-recurrence group according to whether they recurred after surgery. The general data, ADC values, and serum tumor marker levels of the two groups were compared. The correlation between the above indicators and recurrence and metastasis was analyzed by linear regression analysis, and ROC curves were drawn to analyze the predictive value. Results: Compared with the control group, the ADC values and serum CEA, CA199, and CA724 levels in the case group were higher (P<0.05). After radical resection, 15 cases of recurrence and metastasis were found in the case group, accounting for 18.75%. The ADC values in the recurrence group were lower than those in the non-recurrence group, and the serum tumor marker levels were all higher than those in the non-recurrence group (P<0.05). There were no significant differences in the remaining clinical data (P>0.05). Linear regression analysis showed that the ADC value and serum tumor marker level were closely related to postoperative recurrence and metastasis (P<0.05). After drawing the ROC curve, the AUC values of CEA, CA199, CA724, and ADC for predicting postoperative recurrence and metastasis were 0.813, 0.815, 0.755, and 0.838, respectively. The AUC of the joint prediction was 0.907. Conclusion: The ADC value of patients with postoperative recurrence and metastasis of colorectal cancer is low, and the serum tumor marker level is high. The expression of these indicators is closely related to the postoperative recurrence and metastasis of patients with colorectal cancer. Joint detection can effectively improve the value of predicting postoperative recurrence and metastasis in patients with colorectal cancer. 2024 Vol. 30 (7): 1171-1176 [Abstract] ( 26 ) HTML (1 KB)  PDF (1314 KB)  ( 13 ) 1176 Effects of Laparoscopic Unipolar and Bipolar Electrocautery and Harmonic Scalpel Hemostasis on Sex Hormones, Short-Term Ovarian Function, and Immune Function in Patients Undergoing Laparoscopic Total Hysterectomy GUI Dandan, LU Bin, TANG Shiqing DOI: 10.3969/j.issn.1006-6233.2024.07.022 Objective: To analyze the effects of laparoscopic unipolar and bipolar electrocautery and harmonic scalpel hemostasis on sex hormones, short-term ovarian function, and immune function in patients undergoing laparoscopic total hysterectomy. Methods: A total of 105 patients undergoing laparoscopic total hysterectomy in our hospital from October 2020 to December 2023 were selected and randomly divided into a unipolar and bipolar electrocautery group (n=53) and a harmonic scalpel group (n=52) using a simple random sampling method. The two groups were compared in terms of surgical indicators, sex hormones [luteinizing hormone (LH), follicle-stimulating hormone (FSH), estradiol (E2)], ovarian function [maximum diameter (MOD), antral follicle count (AFC), ovarian artery peak systolic blood flow velocity (PSV)], immune function, and complications. Results: There was a statistically significant difference in intraoperative blood loss between the two groups (P<0.05). There was no statistically significant difference in the preoperative levels of LH, FSH, and E2 between the two groups (P>0.05). Postoperatively, LH and FSH levels increased in both groups compared to preoperative levels, and E2 levels decreased significantly in both groups compared to preoperative levels. Postoperatively, the differences in LH, FSH, and E2 between the unipolar and bipolar electrocautery group and the harmonic scalpel group were greater than those in the harmonic scalpel group (P<0.05). There was no statistically significant difference in MOD and PSV between the two groups preoperatively, and there was no statistically significant difference in AFC between the control group pre- and postoperatively (P>0.05). Postoperatively, MOD and PSV decreased in both groups compared to preoperative levels. Postoperatively, the differences in MOD, AFC, and PSV between the unipolar and bipolar electrocautery group and the harmonic scalpel group were greater than those in the harmonic scalpel group (P<0.05). There was no statistically significant difference in the preoperative levels of CD3+, CD4+, and CD8+ between the two groups (P>0.05). Postoperatively, CD3+, CD4+, and CD8+ levels decreased in both groups compared to preoperative levels. Postoperatively, the differences in CD3+, CD4+, and CD8+ between the unipolar and bipolar electrocautery group and the harmonic scalpel group were greater than those in the harmonic scalpel group (P<0.05). The incidence of menstrual abnormalities in the harmonic scalpel group was lower than that in the unipolar and bipolar electrocautery group (P<0.05). Conclusion: Laparoscopic unipolar and bipolar electrocautery and harmonic scalpel hemostasis have certain effects on sex hormones, short-term ovarian function, and immune function in patients undergoing laparoscopic total hysterectomy. However, the choice of hemostasis method should be based on the specific patient and the type and scale of the surgery. 2024 Vol. 30 (7): 1176-1181 [Abstract] ( 18 ) HTML (1 KB)  PDF (1165 KB)  ( 9 ) 1181 Relationship between Serum Sex Hormone Levels and Idiopathic Scoliosis in Adolescent Females HAN Xue, et al DOI: 10.3969/j.issn.1006-6233.2024.07.023 Objective: To investigate the relationship between serum sex hormone levels and idiopathic scoliosis (AIS) in adolescent girls, as well as the relationship between sex hormones and the degree of scoliosis and bone mineral density (BMD). The feasibility and credibility of using sex hormones to identify the probability of AIS in adolescent girls were also analyzed. Methods: Thirty-eight adolescent females with AIS (AIS group) treated in our rehabilitation center from February 2018 to December 2023 were selected, and 29 age-matched females without AIS were recruited as the control group. Serum levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), progesterone, estradiol (E2), Cobb angle, trunk rotation angle, and BMD were measured. The correlation between sex hormone levels and BMD, scoliosis severity in AIS patients, and the value of using sex hormone levels to judge the probability of AIS in adolescent females were analyzed. Results: The serum LH level, Cobb angle, and trunk rotation angle in the AIS group were higher than those in the control group (t=4.532, 42.835, 23.808, P<0.05), while E2 and BMD were lower than those in the control group (t=2.814, 7.082, P<0.05). Serum LH in AIS patients was positively correlated with Cobb angle and trunk rotation angle (P<0.05) and negatively correlated with BMD (P<0.05). E2 levels were negatively correlated with Cobb angle and trunk rotation angle (P<0.05) and positively correlated with BMD (P<0.05). The area under the curve (AUC) for identifying AIS in adolescent females based on E2 and LH was 0.690 and 0.801, respectively. The AUC for identifying AIS in adolescent females based on a combination of E2 and LH was 0.807, which had an identification value (P<0.05), but the difference was not significant compared with the identification value of E2 or LH alone (P>0.05). Conclusion: Sex hormone levels are related to the occurrence of AIS in adolescent females and play an important role in the pathogenesis and progression of the disease. Observation of E2 and LH data alone or in combination can help to identify AIS and assess the severity of the condition. 2024 Vol. 30 (7): 1181-1185 [Abstract] ( 27 ) HTML (1 KB)  PDF (1311 KB)  ( 11 ) 1185 Efficacy of Angiotensin Receptor-Neprilysin Inhibitor Combined with Enhanced External Counterpulsation After Percutaneous Coronary Intervention and Its Impact on Non-invasive Hemodynamic Parameters and Readmission Rate WANG Fangming, WANG Xiang, QI Huaxin DOI: 10.3969/j.issn.1006-6233.2024.07.024 Objective: To investigate the efficacy of angiotensin receptor-neprilysin inhibitor (ARNI) combined with enhanced external counterpulsation (EECP) after percutaneous coronary intervention (PCI) and its impact on non-invasive hemodynamic parameters and readmission rate. Methods: One hundred patients with acute anterior myocardial infarction who successfully underwent emergency PCI treatment at the Department of Cardiology, Shandong First Affiliated Hospital of Shandong University from October 2022 to March 2024 were randomly divided into a trial group and a control group (50 patients each). The trial group received ARNI combined with EECP treatment, and the control group received angiotensin-converting enzyme inhibitor (ACEI) treatment. The two groups were compared in terms of cardiac function, quality of life scale (SF-36), hemodynamic parameters, and readmission rate. Results: Before treatment, there was no significant difference in cardiac function and hemodynamic parameters between the two groups (P>0.05). After 7 weeks of treatment, the levels of LVEDV, LVESV, NT-proBNP, ET-1, heart rate, SBP, DBP, RS, and CMR were decreased, while LVEF, CO, CI, LSI, LCI, AC, and COM were increased in both groups compared to before treatment (P<0.05). The magnitude of change was greater in the trial group than in the control group, and there was a significant difference between the two groups in these parameters (P<0.05). At 6-month follow-up, LVEDV, LVESV, NT-proBNP, ET-1, heart rate, SBP, DBP, RS, and CMR continued to decrease, while LVEF, CO, CI, LSI, LCI, AC, and COM continued to increase. However, the magnitude of change was smaller, and there was still a significant difference between the two groups in these parameters (P<0.05). Before treatment, there was no significant difference in the scores of physical function, physical function, emotional function, daily activities, mental health, overall health, social function, and physical pain on the SF-36 between the two groups (P>0.05). After 7 weeks of treatment, the scores of all of these items increased compared to before treatment (P<0.05), and the magnitude of change was greater in the trial group than in the control group. There was also a significant difference between the two groups in these items (P<0.05). At 6-month follow-up, the scores of these items continued to increase, but the magnitude of change was smaller. There was still a significant difference between the two groups in these items (P<0.05). The total major adverse cardiovascular event (MACE)-related readmission rate at 6 months after treatment was significantly different between the two groups (χ2=9.502, P=0.002). There was no significant difference in the readmission rate due to recurrent myocardial infarction or angina pectoris (χ2=0.340, P=0.560; χ2=2.152, P=0.142), but the readmission rate due to heart failure was significantly different (χ2=6.353, P=0.012). Conclusion: ARNI combined with EECP after PCI can improve cardiac function and patient health status, reduce resistance, reduce the incidence of adverse reactions after surgery, and thus reduce the readmission rate. 2024 Vol. 30 (7): 1185-1191 [Abstract] ( 15 ) HTML (1 KB)  PDF (1179 KB)  ( 21 ) 1191 Differences in Gut Microbiota Characteristics and Clinical Significance between Middle-aged and Young Stroke Patients with and without Hyperhomocysteinemia WANG Xueming, ZHOU TiaoHua, LI LiTing, et al DOI: 10.3969/j.issn.1006-6233.2024.07.025 Objective: To investigate the differences in gut microbiota characteristics and clinical significance between middle-aged and young stroke patients with and without hyperhomocysteinemia (Hcy). Methods: A total of 110 middle-aged and young stroke patients were enrolled as the study group from January 2022 to December 2023. According to the presence or absence of hyperhomocysteinemia, they were divided into the hyperhomocysteinemia (Hcy) subgroup and the normal Hcy subgroup. 55 healthy volunteers who underwent physical examination during the same period were selected as the control group. The incidence of gut microbiota dysbiosis, Alpha diversity, and relative abundance of the research group and control group, the research group subgroups were compared. The relationship between gut microbiota and hyperhomocysteinemia, and stroke was analyzed, and the predictive value of gut microbiota for stroke with hyperhomocysteinemia was evaluated. Results: The incidence of gut microbiota dysbiosis was significantly higher in the Hcy group (52.73%) than in the non-Hcy group (18.18%). The Simpson index (417.61±66.51) was higher in the Hcy group than in the non-Hcy group (668.39±91.08), while the Chaol index (0.96±0.06), Shannon index (2.13±0.52) were lower in the Hcy group than in the non-Hcy group (0.90±0.03), (4.67±0.45) (χ2=18.061, t=20.103, 6.985, 30.891, P<0.001). The abundance of Firmicutes was lower in the Hcy group than in the non-Hcy group, while the abundance of Bacteroidetes and Proteobacteria was higher in the Hcy group (P<0.05). The abundance of Bacteroides genus was higher in the Hcy group than in the non-Hcy group, while the abundance of Bifidobacterium genus, Faecalibacterium genus, and Collinsella genus was lower in the Hcy group (P<0.05). The incidence of gut microbiota dysbiosis was significantly higher in the high-Hcy subgroup (80.00%) than in the normal-Hcy subgroup (33.85%). The Simpson index (383.43±53.18) was higher in the high-Hcy subgroup than in the normal-Hcy subgroup (441.27±65.39), while the Chaol index (0.99±0.05), Shannon index (2.02±0.18) were lower in the high-Hcy subgroup than in the normal-Hcy subgroup (0.94±0.03), (2.21±0.25) (χ2=22.725, t=4.913, 6.545, 4.371, P<0.001). The abundance of Firmicutes was lower in the high-Hcy subgroup than in the normal-Hcy subgroup, while the abundance of Bacteroidetes and Proteobacteria was higher in the high-Hcy subgroup (P<0.05). The abundance of Bacteroides genus was higher in the high-Hcy subgroup than in the normal-Hcy subgroup, while the abundance of Bifidobacterium genus, Faecalibacterium genus, and Collinsella genus was lower in the high-Hcy subgroup (P<0.05). Gut microbiota dysbiosis, Simpson index, and high Hcy were positively correlated with stroke and stroke with Hcy, while Chaol index and Shannon index were negatively correlated with stroke and stroke with Hcy (P<0.05). The area under the curve (AUC) of gut microbiota dysbiosis, Simpson index, Chaol index, and Shannon index for predicting stroke with Hcy in middle-aged and young patients was 0.731 (95%CI: 0.638~0.811), 0.809 (95%CI: 0.723~0878), 0.782 (95%CI: 0.693~0.855), and 0.822 (95%CI: 0.738-0.889), respectively. The sensitivity and specificity were 80.00%, 86.67%, 82.22%, and 75.56%, and 66.15%, 69.23%, 61.54%, and 75.38%, respectively. Conclusion: The incidence of intestinal flora imbalance in middle-aged and young stroke patients with hyperHCY is higher, the diversity is decreased, and the abundance is also significantly changed, which can be used as an auxiliary index to predict stroke patients with hyperHcy, and provide a new perspective for clinical practice. 2024 Vol. 30 (7): 1191-1197 [Abstract] ( 26 ) HTML (1 KB)  PDF (1328 KB)  ( 11 ) 1197 Relationship between Serum BNP TFC CO and Scr Levels and Short-Term Prognosis in Patients with Systolic Heart Failure DONG Junhui, ZHAO Chen, CHEN Wenxin, et al DOI: 10.3969/j.issn.1006-6233.2024.07.026 Objective: To explore the relationship between serum BNP, TFC, CO, Scr and the short-term prognosis of patients with systolic dysfunction heart failure. Methods: A total of 93 patients with systolic dysfunction (LVEF<50%) heart failure admitted to the cardiovascular Department of Chengde Central Hospital from November 2021 to January 2023 were selected. Exacerbation of signs and symptoms of heart failure, cardiovascular re-visits and/or re-hospitalization, and cardiac death were recorded within 90 days after discharge. They were divided into two groups according to the prognosis: good prognosis group and bad prognosis group. Results: A total of 93 patients were followed up, 31 of whom had poor prognosis. LVEF, CO and the prevalence of coronary atherosclerotic heart disease in the poor prognosis group was lower than that in the good prognosis group, and the difference was statistically significant (P<0.05). BNP, cTnI, TFC and Scr in the poor prognosis group were higher than those in the good prognosis group, and the difference was statistically significant (P<0.05). Among them, BNP,TFC, CO and Scr are the factors influencing the adverse prognosis of patients with systolic dysfunction heart failure within 90 days after discharge(P<0.05). Conclusion: Volume load evaluation indexes BNP,TFC, CO and Scr are influential factors for the short-term prognosis of patients with systolic dysfunction heart failure, and may have a predictive effect on their prognosis, and have guiding significance for clinical treatment of patients with heart failure. 2024 Vol. 30 (7): 1197-1201 [Abstract] ( 27 ) HTML (1 KB)  PDF (1137 KB)  ( 19 ) 1202 Correlation Analysis of Lipid Metabolism Index Serum Uric Acid and Urinary Microalbumin Level in Patients with Phlegm-dampness Constitution and Hyperlipidemia Complicated with Depression MIN Yan, et al DOI: 10.3969/j.issn.1006-6233.2024.07.027 Objective: To analyze the correlation of lipid metabolism, serum uric acid and urinary microalbumin in patients with phlegm-dampness constitution and hyperlipidemia complicated with depression. Methods: The clinical data of 157 patients with hyperlipidemia complicated with depression admitted to our hospital from July 2021 to July 2023 were retrospectively analyzed. In addition, 120 healthy people who came to the hospital for physical examination at the same time were selected as the control group. The general demographic data and laboratory test indicators [total cholesterol (TC), triacylglycerol (TG), low density lipoprotein (LDL), high density lipoprotein (HDL), apolipoprotein A (apoA), apolipoprotein B (apoB), uric acid (UA), microalbumin (mAlb)] were compared between both groups. According to the degree of depression, the patients were divided into mild depression group (n=68), moderate depression group (n=56) and severe depression group (n=33), and the levels of UA and mAlb were compared among the three groups. By means of hyperlipidemia typing, the patients were divided into type Ⅰ group (n=25), type Ⅱ group (n=43), type Ⅲ group (n=48), type Ⅳ group (n=28) and type Ⅴ group (n=13). The levels of UA and mAlb among the five groups were compared. Pearson correlation was used to analyze the correlation between lipid metabolism, UA and mAlb. Results: TC, TG, LDL-C, apoA, apoB, UA, mAlb were significantly higher in patients than in control group (P<0.05) and HDL-C was significantly lower in patients than in control group (P<0.05).The levels of UA and mAlb in different degree of depression patients with hyperlipidemia were statistically significant (P<0.05), and the order was mild depression grouP<moderate depression grouP<severe depression group (P<0.05).Among the 157 patients with hyperlipidemia complicated with depression, 25 were type Ⅰ, 43 type Ⅱ, 48 type Ⅲ, 28 type Ⅳ and 13 type Ⅴ.The levels of UA and mAlb in different types of hyperlipidemia patients with depression were statistically significant (P<0.05), and the order was type Ⅰ grouP<type Ⅱ grouP<type Ⅲ grouP<type Ⅳ grouP<type Ⅴ group (P<0.05). Pearson correlation analysis showed that UA and mAlb were not significantly correlated with TC, TG, LDL-C and apoA (P>0.05), but were negatively correlated with HDL-C (r=-0.431, -0.260, P<0.05) and positively correlated with apoB (r=0.413, 0.428, P<0.05), and UA had positive correlation with mAlb (r=0.522, P<0.05). Conclusion: Serum lipid metabolism indexes and UA and mAlb expression levels are highly expressed in patients with hyperlipidemia complicated with depression, and they are closely related to hyperlipidemia complicated with depression. 2024 Vol. 30 (7): 1202-1206 [Abstract] ( 26 ) HTML (1 KB)  PDF (1140 KB)  ( 17 ) 1206 Clinical Significance of Interferon-γ FM and MP-Ab Detection in Children with Mycoplasma Pneumoniae Infection GAO Lihua, ZHU Li, MAN Haixia DOI: 10.3969/j.issn.1006-6233.2024.07.028 Objective: To investigate the clinical significance of interferon-γ (IFN-γ), fibrin monomer (FM), and Mycoplasma pneumoniae antibody (MP-Ab) detection in children with Mycoplasma pneumoniae infection. Methods: A total of 103 children with Mycoplasma pneumoniae pneumonia admitted to our hospital from November 2020 to November 2023 were enrolled as the study group. Sixty healthy children who underwent physical examination and showed normal results during the same period were selected as the control group. The serum levels of IFN-γ and FM and the contents of MP-Ab in the two groups were compared. The serum levels of IFN-γ, FM and the contents of MP-Ab in children with different disease severity in the observation group were compared. With pathological examination as the gold standard, the evaluation value of serum IFN-γ, FM and MP-Ab detection for Mycoplasma pneumoniae pneumonia was analyzed. Results: Among the 103 children with Mycoplasma pneumoniae pneumonia, 72 were in the acute stage and 31 were in the recovery stage. Among the 72 children in the acute stage, 18 had severe Mycoplasma pneumoniae pneumonia and 54 had mild Mycoplasma pneumoniae pneumonia. The serum levels of IFN-γ and FM and the contents of MP-Ab in the acute and recovery stages of the observation group were significantly higher than those in the control group, and the acute stage was significantly higher than the recovery stage (P<0.05). The serum levels of IFN-γ and FM and the contents of MP-Ab in children with severe Mycoplasma pneumoniae pneumonia in the acute stage were significantly higher than those in children with mild Mycoplasma pneumoniae pneumonia, and the differences were statistically significant (P<0.05). Conclusion: The levels of IFN-γ, FM and MP-Ab are highly expressed in children with Mycoplasma pneumoniae pneumonia and increase gradually with the aggravation of the disease. They have the potential to be applied to the early diagnosis and evaluation of the disease in children. 2024 Vol. 30 (7): 1206-1210 [Abstract] ( 21 ) HTML (1 KB)  PDF (1132 KB)  ( 11 ) 1210 Establishment of a Prognostic Prediction Model for Extensive-Stage Small Cell Lung Cancer Based on 18F-FDG PET/CT FENG Zhao, et al DOI: 10.3969/j.issn.1006-6233.2024.07.029 Objective: To investigate the prognostic factors of extensive-stage small cell lung cancer (ES-SCLC) and establish a prognostic prediction model based on 18F-FDG PET/CT, to provide more references for subsequent clinical prognosis prediction. Methods: A total of 142 patients with ES-SCLC who underwent 18F-FDG PET/CT examination in our hospital from January 2017 to January 2020 were retrospectively enrolled. The clinical characteristics, 18F-FDG PET/CT examination indicators and overall survival follow-up information were analyzed. Univariate and multivariate analyses of prognostic factors for ES-SCLC were performed. Establishment and prediction performance analysis of the prognostic prediction model for ES-SCLC based on 18F-FDG PET/CT. Results: The 142 patients were followed up for 15-34 months, with a median follow-up time of 24.0 months. A total of 85 patients died during follow-up, and the 1-year and 2-year overall survival rates were 59.2% and 34.0%, respectively. According to the patients' age, bone metastasis, liver metastasis, total MTV of the whole body, and total TLG of the whole body, the patients were divided into two groups. There was a statistically significant difference in the median overall survival time between the subgroups (P<0.05). Univariate and multivariate COX regression analysis results showed that age, whether there was bone and liver metastasis, total MTV of the whole body 2.5 and total TLG of the whole body 2.5 were all independent prognostic factors for overall survival of patients with ES-SCLC (P<0.05). A line chart model of the survival probability of patients at different time periods was constructed based on each independent risk factor, and the C-index was 0.763. The ROC curve was used to calculate the prediction of the survival status of patients at 1, 2, and 3 years, respectively, and the area under the curve was 0.631, 0.718, and 0.722, respectively. Conclusion: The prognosis of ES-SCLC is closely related to 18F-FDG PET/CT indicators in addition to age and bone metastasis; using the above indicators to predict the survival status of patients has a good effect. 2024 Vol. 30 (7): 1210-1215 [Abstract] ( 33 ) HTML (1 KB)  PDF (1263 KB)  ( 9 ) 1216 Efficacy of Pioglitazone Combined with Liraglutide in the Treatment of Obese Type 2 Diabetes Mellitus and Its Effect on Islet Function and NLRP3 Inflammasome XU QiuJin, et al DOI: 10.3969/j.issn.1006-6233.2024.07.030 Objective: To investigate the efficacy of pioglitazone combined with liraglutide in the treatment of obese type 2 diabetes mellitus (T2DM), and to analyze the effects of pioglitazone combined with liraglutide on islet function and the inflammasome of nucleotide-binding oligomerized domain-like receptor protein 3 (NLRP3). Methods: A retrospective study was conducted on 116 obese patients with type 2 diabetes mellitus (T2DM) from July 2021 to April 2023. The patients were randomly divided into a control group (liraglutide, 58 cases) and a study group (pioglitazone combined with liraglutide, 58 cases) according to a random number table method, and treated continuously for 3 months. The therapeutic efficacy of the two groups was compared. Blood samples were collected before and after treatment for 3 months in both groups, and the blood glucose and lipid metabolism were detected using an XC8001 automatic biochemical analyzer. The fasting insulin level was detected using immunochromatography (the kit was purchased from Shanghai Kanglang Biotechnology Co., Ltd.), and the insulin resistance index (HOMA-IR) and insulin beta cell function index (HOMA-β) were calculated. The level of NLRP3 inflammatory body index was detected using real-time quantitative polymerase chain reaction (qRT-PCR). The levels of serum interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and C-reactive protein (CRP) were detected using ELISA before and after treatment in both groups. The adverse reactions of the two groups were compared. Results: The total effective rate in the study group was 94.83%, which was higher than the 81.03% in the control group (χ2=5.199, P=0.023). Three months after treatment, The levels of fasting blood glucose (FBG), 2 h postprandial glucose (2 hPG), glycated hemoglobin (HbA1c), total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) in the study group were (4.82±0.56) mmol/L, (11.24±2.04) mmol/L and (5.47±0.61)%, (3.16±0.33) mmol/L, (1.31±0.30) mmol/L, (2.01±0.34) mmol/L, Were lower than (5.57±0.74) mmol/L, (13.07±2.16) mmol/L, (6.73±0.68)%, (3.89±0.54) mmol/L, (1.82±0.35) mmol/L, (2.39±0.42) mmol/L in control group (P<0.05). After 3 months of treatment, the level of high-density lipoprotein cholesterol (HDL-C) in the study group (1.38±0.22) mmol/L was higher than that in the control group (1.14±0.19) mmol/L (P<0.05). After 3 months of treatment, the insulin resistance index (HOMA-IR) of the study group (2.16±0.37) was lower than that of the control group (2.58±0.46), and the insulin beta cell function index (HOMA-β) of the study group (48.20±6.03) was higher than that of the control group [(42.81±5.54), (P<0.05)]. After 3 months of treatment, the expression levels of NLRP3 mRNA, apoptosis-related speck-like protein (ASC) mRNA and Caspase-1 mRNA in PBMC of the study group were (1.03±0.32), (1.11±0.28) and (1.09±0.41), respectively, lower than control group [(1.65±0.38), (1.57±0.30), (1.72±0.49), (P<0.05)]; Three months after treatment, Serum levels of interleukin-6 (IL-6), interleukin-β (IL-1β), tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP) in the study group were (8.55±2.13) pg/mL, (35.24±5.93) ng/L, (11.42±2.64) ng/L, (1.0 8±0.31 mg/L was lower than that of control group (11.47±2.68) pg/mL, (42.53±7.06) ng/L, (15.03±3.18) ng/L, (1.46±0.44) mg/L (P<0.05). There was no significant difference in adverse reactions between the two groups (P>0.05). Conclusion: Pioglitazone combined with liraglutide is effective and safe in the treatment of obese T2DM. It can correct the disorder of glucose and lipid metabolism, promote the improvement of islet function, and inhibit inflammatory response, which may be related to inhibiting the activation of NLRP3 inflammasome. 2024 Vol. 30 (7): 1216-1222 [Abstract] ( 27 ) HTML (1 KB)  PDF (1168 KB)  ( 10 ) 1222 Application of Nicardipine Combined with rh-proUK in Percutaneous Coronary Intervention for Chronic Total Occlusion of Coronary Artery PU Mingyu, ZHENG Xi, HE Lin DOI: 10.3969/j.issn.1006-6233.2024.07.031 Objective: To investigate the effect of targeted intracoronary injection of nicardipine combined with recombinant human pro-urokinase (rh-proUK) on the treatment of chronic total occlusion (CTO) of coronary artery and its impact on long-term prognosis. Methods: A total of 237 cases of CTO patients who underwent PCI in our hospital from September 2021 to January 2023 were collected as the study objects and a retrospective study was carried out. Patients treated with nicodil combined with PCI through targeted catheter were included in Nicodil group (n=78), and patients treated with rh-proUK combined with PCI through a targeted catheter were included in the rh-proUK group (n=78). Patients treated with nicodil and rh-proUK combined PCI via targeted catheter were included in the combination group (n=81). The microcirculation indexes [TIMI blood flow grading, corrected TIMI blood flow frame count (cTFC), TIMI myocardial perfusion grading (TMPG)], myocardial injury indexes [creatinine, N-terminal brain natriuretic peptide precursor (NT-proBNP), creatine kinase isoenzyme (CK-MB), and troponin (IcTnI)], cardiac function [left ventricular ejection fraction (LVEF), ventricular wall motion integral index (WMSI), left ventricular end-diastolic inner diameter (LVED)], myocardial microcirculation blood perfusion [myocardial perfusion quantitative analysis constant (k)], and incidence of adverse cardiovascular events were compared between the three groups. Results: The proportion of TMPG grade 3 in the combination group was higher than that in the Nicodil group and rh-proUK group, and cTFC was lower than that in the Nicodil group and rh-proUK group (P<0.05). 24h after the operation, CK-MB, creatinine, IcTnI and NT-proBNP in the combination group were lower than those in nicodil and rh-proUK groups (P<0.05). Three months after the operation, LVEF in the combination group was higher than that in nicodil group and rh-proUK group, and LVED was lower than that in nicodil group and rh-proUK group (P<0.05). The k value of myocardial microcirculation perfusion in basal state and load state in the combination group was higher than that in the nicodil group and rh-proUK group (P<0.05). There was no significant difference in the total incidence of adverse cardiovascular events at 6 months and 12 months after surgery among 3 groups (P>0.05). Conclusion: Targeted catheter injection of nicodil and rh-proUK combined PCI in the treatment of CTO patients can promote postoperative myocardial microcirculation improvement, help alleviate postoperative myocardial injury, restore cardiac function, reduce adverse cardiovascular events, and improve long-term prognosis. 2024 Vol. 30 (7): 1222-1228 [Abstract] ( 22 ) HTML (1 KB)  PDF (1173 KB)  ( 17 )

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