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2024 Vol. 30, No. 6 Published: 2024-06-30 881 circ-RBM33 Targets the miR-383-3p/CKS1B Axis to Inhibit Proliferation and Invasion of Nasopharyngeal Carcinoma Cells ZHOU Guangquan, SUN Xiangbo, LU Haibo, et al DOI: 10.3969/j.issn.1006-6233.2024.06.01 Objective: To investigate whether circ-RBM33 can target the miR-383-3p/CKS1B axis to inhibit the proliferation and invasion of nasopharyngeal carcinoma cells.Methods: qRT-PCR was used to detect the expression of circ-RBM33 and miR-383-3p in CNE1 and HNEpC cells. CNE1 cells were randomly divided into si-NC group, si-RBM33 group, miR-NC group, miR-383-3p group, and si-RBM33 + anti-miR-383-3p group. Cell proliferation (CCK-8 assay), invasion (Transwell assay), migration (scratch assay), and expression of CKS1B protein in cells (Western blot) were detected. Luciferase activity assay was performed to determine the targeting relationship between circ-RBM33 and miR-383-3p/CKS1B.Results: The relative expression of circ-RBM33 in human nasopharyngeal carcinoma CNE1 cells was higher than that in human nasopharyngeal mucosal epithelial HNEpC cells, while the relative expression of miR-383-3p was lower than that in HNEpC cells (P<0.05). In the si-RBM33 group, the relative expression of circ-RBM33 in CNE1 cells, cell viability, number of invading cells, scratch closure rate, and expression of CKS1B protein in cells were lower than those in the si-NC group (P<0.05). In the si-RBM33 + anti-miR-383-3p group, the cell viability, number of invading cells, scratch closure rate, and expression of CKS1B protein in cells were higher than those in the si-RBM33 group (P<0.05). In the miR-383-3p group, the relative expression of miR-383-3p in CNE1 cells was significantly increased, while cell viability, number of invading cells, scratch closure rate, and expression of CKS1B protein in cells were lower than those in the miR-NC group (P<0.05). Transfection with WT-circ-RBM33/CKS1B resulted in significantly lower luciferase activity in the miR-383-3p group than in the miR-NC group (P<0.0001).Conclusion: Knockdown of circ-RBM33 may inhibit the proliferation, invasion, and migration of nasopharyngeal carcinoma cells by upregulating miR-383-3p expression and subsequently suppressing CKS1B expression. 2024 Vol. 30 (6): 881-886 [Abstract] ( 56 ) HTML (1 KB)  PDF (2099 KB)  ( 28 ) 886 Mechanism Study on Ang -Ⅱ Mediated Ferroptosis in Triggering Atrial Fibrillation and Progression of Heart Failure WANG Xinting, XU Mengmeng, KONG Xue, et al DOI: 10.3969/j.issn.1006-6233.2024.06.02 Objective: To investigate the role of lysine demethylase 5B(KDM5B)/ enhanced activated transcription factor 3(ATF3) signaling pathway in angiotensin Ⅱ (Ang-Ⅱ)-induced cardiac hypertrophy.Methods: Mouse cardiomyocytes HL-1 were divided into the following treatment groups: control group, Ang-Ⅱ group, Ang-Ⅱ+Lv-NC group, Ang-Ⅱ+Lv-KDM5B group and Ang-Ⅱ+LV-KDM5KD group. The expression of KDM5B and ATF3 protein in cells of each group was analyzed by protein blot. The intracellular levels of α -actin (α-SMA), Fe2+ and reactive oxygen species (ROS) were detected by fluorescence staining. Results: Compared with Ang-Ⅱ+Lv-NC group, the expression of KDM5B protein in HL-1 cells in Ang-Ⅱ+Lv-KDM5B group increased significantly (P<0.05), and the expression of ATF3 protein decreased significantly (P<0.05), while the expression of KDM5B protein in HL-1 cells in Ang-Ⅱ+Lv-KDM5B KD group decreased significantly (P<0.05). Compared with the control group, the relative cell size, intracellular relative Fe2+ fluorescence intensity and relative ROS fluorescence intensity in Ang-Ⅱ group and Ang-Ⅱ+Lv-NC group increased significantly (P<0.05), and the relative cell viability decreased significantly (P<0.05). Compared with Ang-Ⅱ+Lv-NC group, the relative cell size, intracellular relative Fe2+ fluorescence intensity and relative ROS fluorescence intensity in Ang-Ⅱ+Lv-KDM5B group increased significantly (P<0.05), while the relative cell size, intracellular relative Fe2+ fluorescence intensity and relative ROS fluorescence intensity in Ang-Ⅱ+Lv-KDM5B KD group decreased significantly (P<0.05).Conclusion: KDM5B/ATF3 drives the progress of Ang-Ⅱ-induced myocardial hypertrophy and promotes ferroptosis related to hypertrophic stress response. 2024 Vol. 30 (6): 886-892 [Abstract] ( 37 ) HTML (1 KB)  PDF (2037 KB)  ( 19 ) 892 Gut Microbiota Metabolite Trimethylamine N-oxide Promotes Atherosclerosis by Inhibiting the Keap1/Nrf2 Signaling Pathway SHAN Siyang, et al DOI: 10.3969/j.issn.1006-6233.2024.06.03 Objective: To investigate the effects of the gut microbiota (GM) metabolite trimethylamine N-oxide (TMAO) on atherosclerosis (AS) and its related mechanisms.Methods: Male mice were randomly divided into the control group, model group, TMAO group, Keap1/Nrf2 activator RTA-408 group, and TMAO+RTA-408 group, with 12 mice in each group. The model group mice were fed a high-fat diet, while the TMAO group mice were fed a high-fat diet with 1% choline for a modeling period of 12 weeks. After modeling, mice in the RTA-408 and TMAO+RTA-408 groups received a single daily intraperitoneal injection of RTA-408 (100 μg/kg) for 14 days, while mice in other groups received equivalent amounts of saline. Biochemical analysis was used to quantify the levels of TG, TC, LDL-C, and HDL-C. Histological changes in the aorta were detected using HE, Masson trichrome, and oil red O staining. ELISA was used to detect serum levels of interleukin-1β (IL-1β), reactive oxygen species (ROS), and superoxide dismutase (SOD). Ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to detect plasma TMAO levels in mice. Fluorescent probe assays were used to detect ROS fluorescence intensity in the aorta. qRT-PCR and Western blot were used to detect mRNA and protein expression levels of Keap1, Nrf2, and HO-1 in mouse aortic tissue. Immunofluorescence was used to observe Nrf2 nuclear translocation.Results: Serum TC, TG, and LDL-C concentrations were higher in AS mice compared to the control group, while HDL-C concentration was lower (P<0.01). Additionally, the model group showed extensive aortic intima thickening, significant foam cell formation, and increased collagen deposition in the arterial wall. Serum levels of IL-1β, ROS, and TMAO were significantly elevated (P<0.01), while SOD activity was significantly reduced (P<0.01). Aortic ROS content increased, Nrf2 nuclear translocation was significantly inhibited (P<0.01), and mRNA and protein expression levels of Keap1, Nrf2, and HO-1 increased (P<0.01). Compared to AS mice, TMAO treatment further aggravated the changes in these indicators (P<0.05); RTA-408, however, negated the exacerbating effects of TMAO on AS mice (P<0.05).Conclusion: TMAO may exacerbate aortic pathological changes, inflammatory responses, and endothelial injury in AS mice by inhibiting the activation of the Keap1/Nrf2 signaling pathway. 2024 Vol. 30 (6): 892-899 [Abstract] ( 25 ) HTML (1 KB)  PDF (2275 KB)  ( 34 ) 900 Effect of Polydatin on Neuroinflammation in Rats with Hypertensive Intracerebral Hemorrhage via Regulation of the JAK2/STAT3 Signaling Pathway XU Yongjie, JIA Xiaolong, WANG Yanling, et al DOI: 10.3969/j.issn.1006-6233.2024.06.04 Objective: To investigate the effect of polydatin on neuroinflammation in rats with hypertensive intracerebral hemorrhage (HICH) through the regulation of the JAK2/STAT3 signaling pathway.Methods: Sprague-Dawley rats were randomly divided into six groups: sham operation group, HICH group, and three polydatin dosage groups (low, medium, and high; Polydatin-L, Polydatin-M, Polydatin-H; 25mg/kg, 50mg/kg, 100mg/kg respectively), and the Polydatin-H + JAK2/STAT3 pathway activator (Colivelin) group (100mg/kg Polydatin + 1 mg/kg Colivelin), with 10 rats in each group. The sham operation group underwent blunt separation of bilateral renal arteries and injection of saline, while the other groups were induced with HICH by bilateral renal artery constriction using silver clips and autologous blood injection, followed by respective drug or saline interventions. Three days later, modified Neurological Severity Score (mNSS) and brain water content were measured. Serum was isolated for ELISA to detect levels of interleukin (IL)-1β and tumor necrosis factor (TNF)-α. Brain tissue was isolated for pathological changes observed by HE staining, IL-10 and IL-4 expression detected by immunofluorescence, and JAK2/STAT3 signaling pathway-related protein expression analyzed by Western blot.Results: Compared with the sham operation group, the HICH group showed significantly increased mNSS score, serum IL-1β, TNF-α levels, brain water content, p-JAK2/JAK2, and p-STAT3/STAT3 expressions, but decreased IL-10 and IL-4 expressions (P<0.05). Compared with the HICH group, the Polydatin-L, Polydatin-M, and Polydatin-H groups had significantly decreased mNSS scores, serum IL-1β, TNF-α levels, brain water content, p-JAK2/JAK2, and p-STAT3/STAT3 expressions, but increased IL-10 and IL-4 expressions, with statistically significant differences among the different concentrations of polydatin (P<0.05). Compared with the Polydatin-H group, the Polydatin-H + Colivelin group had significantly increased mNSS score, serum IL-1β, TNF-α levels, brain water content, p-JAK2/JAK2, and p-STAT3/STAT3 expressions, but decreased IL-10 and IL-4 expressions (P<0.05).Conclusion: Polydatin inhibits the JAK2/STAT3 signaling pathway, reduces pro-inflammatory cytokine levels, enhances anti-inflammatory levels, and alleviates pathological damage in rats with HICH. 2024 Vol. 30 (6): 900-905 [Abstract] ( 26 ) HTML (1 KB)  PDF (3187 KB)  ( 12 ) 905 Effects of Quercetin on Vascular Endothelial Function and Aortic Fibrosis in Spontaneously Hypertensive Rats LI Xinfeng, LU Guanghui, et al DOI: 10.3969/j.issn.1006-6233.2024.06.05 Objective: To investigate the effects of quercetin on vascular endothelial function and aortic fibrosis in spontaneously hypertensive rats (SHR), and to explore its possible mechanisms.Methods: Ten Wistar-Kyoto (WKY) rats were set as the normal group; forty SHRs were randomly divided into the model group, low-dose quercetin group, high-dose quercetin group, and captopril group. The low-dose and high-dose quercetin groups were administered quercetin via gavage at doses of 50mg/kg and 100mg/kg respectively once a day, while the captopril group was given 30mg/kg captopril once a day. The normal and model groups were given an equal volume of normal saline once a day. After 8 weeks of continuous treatment, blood pressure was measured, and serum levels of endothelin-1 (ET-1), angiotensin Ⅱ (Ang-Ⅱ), thromboxane B2 (TXB2), and nitric oxide (NO) were detected by ELISA. ELISA was also used to measure the contents of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL-6 in the aorta. Hematoxylin and eosin (HE) staining was performed for pathological examination of the aorta, and Masson's trichrome staining was used to observe aortic fibrosis. Western blotting was conducted to detect the expression of Toll-like receptor 4 (TLR4), nuclear factor-κB p65 (NF-κB p65) in the cytoplasm and nucleus, transforming growth factor-β1 (TGF-β1), Smad3, phosphorylated Smad3 (p-Smad3), type I collagen (Col-I), and type Ⅲ collagen (Col-Ⅲ) in the aorta.Results: Compared with the model group, the low-dose quercetin group, high-dose quercetin group, and captopril group showed significantly lower systolic blood pressure (SBP), diastolic blood pressure (DBP), and serum levels of ET-1, Ang-Ⅱ, and TXB2, along with significantly higher serum NO levels (P<0.05). The levels of TNF-α, IL-1β, and IL-6 in the aorta were significantly reduced (P<0.05). Both pathological changes and fibrosis in the aorta were significantly improved, with a notable reduction in collagen volume fraction (CVF) (P<0.05). The expression levels of TLR4, nuclear NF-κB p65, TGF-β1, p-Smad3, Col-I, and Col-Ⅲ proteins, as well as the ratios of nuclear NF-κB p65 to cytoplasmic NF-κB p65 and p-Smad3 to Smad3, were significantly lower (P<0.05). Except for SBP, DBP, and TXB2, the regulatory effects of high-dose quercetin on other indicators were superior to those of the captopril group (P<0.05).Conclusion: Quercetin can improve vascular endothelial function and reduce aortic fibrosis in SHR, lowering blood pressure. Its mechanisms may be related to the inhibition of the TLR4/NF-κB signaling pathway and the TGF-β1/Smad3 signaling pathway. 2024 Vol. 30 (6): 905-912 [Abstract] ( 24 ) HTML (1 KB)  PDF (2352 KB)  ( 9 ) 912 Mechanism of Inhibition of Apoptosis in Respiratory Syncytial Virus-Infected Bronchial Epithelial Cells by Glycyrrhizic Acid FU Xiaokang, LIANG Weiwei, et al DOI: 10.3969/j.issn.1006-6233.2024.06.06 Objective: To explore the effect of glycyrrhizic acid on the apoptosis of bronchial epithelial cells infected with respiratory syncytial virus (RSV) and to analyze whether the mechanism is related to the endogenous Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) signaling pathway.Methods: Human bronchial epithelial (HBE) cells were divided into seven groups: control group, infection group (RSV group), low-dose glycyrrhizic acid group (RSV+L-glycyrrhizic acid group), medium-dose glycyrrhizic acid group (RSV+M-glycyrrhizic acid group), high-dose glycyrrhizic acid group (RSV+H-glycyrrhizic acid group), RSV+Colivelin group, and RSV+H-glycyrrhizic acid+SD-1029 group. The control group cells were untreated. The RSV group cells were cultured with RSV virus solution at 0.0001 PFU/mL for 2 hours and then switched to normal culture medium for 24 hours. The RSV+L-glycyrrhizic acid group, RSV+M-glycyrrhizic acid group, and RSV+H-glycyrrhizic acid group were cultured with media containing glycyrrhizic acid at concentrations of 30, 60, and 120μg/mL respectively after RSV infection. The RSV+Colivelin group cells were cultured with media containing 0.5μmoL/L of JAK2/STAT3 signaling pathway activator Colivelin after RSV infection. The RSV+H-glycyrrhizic acid+SD-1029 group cells were cultured with media containing 120μg/mL glycyrrhizic acid and 10μmoL/L JAK2/STAT3 signaling pathway inhibitor SD-1029 after RSV infection. After 24 hours, cells were collected. Cell viability was detected using the CCK-8 kit. Apoptosis was detected by flow cytometry. Levels of IL-6 and TNF-α in cell supernatants were measured by ELISA. Protein expression of Bax, cleaved-Caspase-3, Bcl-2, p-JAK2, JAK2, p-STAT3, and STAT3 was analyzed by western blot.Results: Compared to the control group, the RSV group showed significantly lower OD value, Bcl-2, cleaved-Caspase-3, p-JAK2/JAK2, and p-STAT3/STAT3 protein expression (P<0.05), and significantly higher cell apoptosis rate, IL-6, TNF-α levels, and Bax protein expression (P<0.05). Compared to the RSV group, the RSV+L-glycyrrhizic acid group, RSV+M-glycyrrhizic acid group, RSV+H-glycyrrhizic acid group, and RSV+Colivelin group showed significantly higher OD value, Bcl-2, cleaved-Caspase-3, p-JAK2/JAK2, and p-STAT3/STAT3 protein expression (P<0.05), and significantly lower cell apoptosis rate, IL-6, TNF-α levels, and Bax protein expression (P<0.05). Compared to the RSV+H-glycyrrhizic acid group, the RSV+H-glycyrrhizic acid+SD-1029 group showed significantly lower OD value, Bcl-2, cleaved-Caspase-3, p-JAK2/JAK2, and p-STAT3/STAT3 protein expression (P<0.05), and significantly higher cell apoptosis rate, IL-6, TNF-α levels, and Bax protein expression (P<0.05).Conclusion: Glycyrrhizic acid may promote the proliferation of bronchial epithelial cells infected with RSV and inhibit their apoptosis by activating the JAK2/STAT3 signaling pathway. 2024 Vol. 30 (6): 912-918 [Abstract] ( 18 ) HTML (1 KB)  PDF (1764 KB)  ( 11 ) 918 The Value of RDW D-dimer Inflammatory Factors and Transthoracic Pulmonary Ultrasound in Evaluating the Prognosis of Sepsis Patients WU Gaofei, et al DOI: 10.3969/j.issn.1006-6233.2024.06.07 Objective: To explore the value of red blood cell distribution width (RDW), D-dimer (D-D), inflammatory factors, and transthoracic lung ultrasound in evaluating the prognosis of sepsis patients.Methods: A retrospective study was conducted on 98 sepsis patients treated in our hospital from January 2020 to October 2023, including 51 patients with simple sepsis and 47 patients with septic shock. There were 26 deceased patients and 72 surviving patients. Clinical data, RDW, D-D, inflammatory factors, and transthoracic lung ultrasound differences were compared among patients with and without shock, and with different prognoses. Factors influencing patient prognosis were analyzed.Results: Sepsis shock patients had RDW, D-D, interleukin-6 (IL-6), C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-alpha), transthoracic lung ultrasound B-line fusion >2 zones ratio, and Acute Physiology and Chronic Health Evaluation Ⅱ (APACHE Ⅱ) scores of 16.68 (14.12, 19.55)%, 3.22 (2.15, 7.78) mg/L, (410.03±102.21) pg/L, (32.21±11.15) mg/L, (503.74±98.87) ng/mL, 70.21%, and (18.84±1.15) points, respectively, which were higher than those of simple sepsis patients (P<0.05). The age, proportion of septic shock, RDW, D-D, IL-6, CRP, TNF-alpha, transthoracic lung ultrasound B-line fusion >2 zones ratio, and APACHE Ⅱ scores of deceased patients were (65.51±8.22) years, 76.92%, 18.87 (15.21, 22.10)%, 3.90 (2.65, 8.10) mg/L, (425.59±98.21) pg/L, (31.14±9.98) mg/L, (522.25±100.18) ng/ml, 88.46%, and (19.11±1.43) points, respectively, which were higher than those of surviving patients (P<0.05). Logistic regression analysis showed that RDW, D-D, IL-6, CRP, TNF-alpha, transthoracic lung ultrasound B-line fusion >2 zones ratio, and APACHE Ⅱ scores were influencing factors for patient mortality (P<0.05). The area under the ROC curve for predicting mortality in sepsis patients using the Logistic model was 0.842 (95%CI: 0.757-0.927), with sensitivity and specificity of 76.00% and 78.10%, respectively.Conclusion: RDW, D-D, IL-6, CRP, TNF-alpha, and transthoracic lung ultrasound B-line fusion are related to whether sepsis patients develop shock and their prognosis, and have certain application value in predicting patient prognosis. 2024 Vol. 30 (6): 918-923 [Abstract] ( 22 ) HTML (1 KB)  PDF (1246 KB)  ( 11 ) 923 Changes and Clinical Significance of Serum Creatine Kinase MB Neuropeptide Y and β2-Microglobulin Levels in Hyperbilirubinemic Neonates LI Jiaqiang, et al DOI: 10.3969/j.issn.1006-6233.2024.06.08 Objective: To investigate the changes and clinical significance of serum creatine kinase isoenzyme (CreatineKinase-MB, CKMB), neuropeptide Y (Neuropeptide Y, NPY), and β2-microglobulin (β2-microglobulin, β2-MG) levels in hyperbilirubinemic neonates.Methods: A total of 118 hyperbilirubinemic neonates admitted to our hospital from January 2019 to January 2022 were selected as the observation group. According to the total bilirubin (STB) level, they were divided into three groups: mild group (n=58, 220.6μmol/L≤STB≤256.5μmol/L), moderate group (n=42,256.5μmol/L≤STB≤342μmol/L), and severe group (n=18, STB>342μmol/L). Another 112 healthy neonates without jaundice who underwent percutaneous bilirubin measurement in our hospital during the same period were selected as the control group. The levels of CKMB, NPY, and β2-MG in the control group and observation group were compared. The levels of CKMB, NPY, and β2-MG in patients with different STB levels in the observation group were compared. According to the percutaneous bilirubin-measured STB level, the patients were divided into the acute phase group and the recovery phase group, and the levels of CKMB, NPY, and β2-MG in the two groups were compared.Results: The levels of CKMB, NPY, and β2-MG in the observation group were all higher than those in the control group (P<0.05). Comparison of CKMB, NPY, and β2-MG levels in patients with different STB levels: mild group < moderate group < severe group (P<0.05). Among the 118 hyperbilirubinemic neonates, there were 67 cases in the acute phase group and 51 cases in the recovery phase group. The levels of CKMB, NPY, and β2-MG in the acute phase group were all higher than those in the recovery phase group (P<0.05).Conclusion: Serum CKMB, NPY, and β2-MG levels are closely related to the severity and prognosis of hyperbilirubinemia in neonates. Monitoring these indicators can help assess the progression and prognosis of the condition, providing significant clinical value. 2024 Vol. 30 (6): 923-927 [Abstract] ( 25 ) HTML (1 KB)  PDF (1186 KB)  ( 12 ) 927 Correlation of Serum ICAM-1 Hcy and MCP-1 Levels with Vascular Endothelial Function in Patients with Retinal Vein Occlusion ZHENG Bo, CHEN Lili DOI: 10.3969/j.issn.1006-6233.2024.06.09 Objective: To investigate the correlation between serum intercellular adhesion molecule-1 (ICAM-1), Homocysteine (Hcy), and Monocyte chemotactic protein-1 (MCP-1) levels and vascular endothelial function in patients with retinal vein occlusion (RVO).Methods: A retrospective, double-blind method was used to analyze 95 patients with RVO admitted to our hospital from July 2020 to October 2023 as the RVO group, and 102 healthy people who underwent a physical examination in our hospital during the same period were selected as the normal control group. To compare the levels of ICAM-1, Hcy, MCP-1, vascular endothelial function [Endothelin-1 (ET-1), nitric oxide (NO)] between the two groups; to compare the levels of ICAM-1, Hcy, MCP-1, ET-1 and NO in the RVO group with different types of diseases; to compare the levels of ICAM-1, MCP-1, ET-1 and NO in the RVO group with different degrees of diseases; and to analyze the correlation between serum levels of ICAM-1, Hcy, MCP-1 and vascular endothelial function in RVO patients.Results: Serum ICAM-1, Hcy, MCP-1, and ET-1 levels were higher and NO levels were lower in the RVO group than in the control group (P<0.05); serum ICAM-1, Hcy, MCP-1, and ET-1 levels were higher and NO levels were lower in the CRVO patients than in the BRVO patients (P<0.05); ICAM-1, MCP-1, and ET 1levels: mild<moderate<severe, NO levels: mild>moderate>severe (P<0.05); serum ICAM-1, Hcy, MCP-1 levels were positively correlated with ET-1 levels and negatively correlated with NO levels (P<0.05).Conclusion: Serum ICAM-1, Hcy and MCP-1 levels are all abnormally elevated in RVO patients, and are correlated with ET-1 and NO to a certain extent. Detection of serum ICAM-1, Hcy and MCP-1 levels can be used to assess the status of vascular endothelial function injury, to facilitate early treatment. 2024 Vol. 30 (6): 927-931 [Abstract] ( 28 ) HTML (1 KB)  PDF (1188 KB)  ( 10 ) 931 γ-linolenic Acid Improves Cognitive Impairment in Mice with Cerebral Infarction by Down-Regulating Oxidative Stress Response and Inhibiting NLRP3-Mediated Pyroptosis SHI Jian, GONG Liya, WANG Yan DOI: 10.3969/j.issn.1006-6233.2024.06.010 Objective: To investigate the effect and mechanism of γ-linolenic acid (GLA) on cognitive impairment in mice with cerebral ischemia. Methods: Eighteen male mice were randomly divided into three groups: control group (n=6), model group (n=6), and GLA group (n=6). The cerebral infarction model was established in the model group and GLA group by middle cerebral artery occlusion (MCAO), and sham surgery was performed in the control group. Mice in the control and model groups were administered saline (1 mg/kg) by gavage daily, while mice in the GLA group were administered GLA (1 mg/kg) by gavage daily for 14 days. Morris water maze test was used to evaluate the cognitive impairment of mice. The infarct area and neuronal apoptosis in the brain tissues were measurd by HE staining and TUNEL staining, respectively. The expression levels of P22, P47, NLRP3, IL-1β, GSDMD, and Caspase-1 proteins in the hippocampus of mice were detected by Western blot. Results: The escape latency and crossing number of mice in the model group were shorter and more than those in the GLA group and control group, respectively. The infarct area and the number of apoptotic neurons in the brain tissues of the model group were the largest, followed by the GLA group, and there was no cerebral infarction or neuronal apoptosis in the control group. The expression levels of P22, P47, NLRP3, IL-1β, GSDMD, and Caspase-1 proteins in the model group were higher than those in the GLA group and control group, while those in the GLA group were higher than those in the control group. Conclusion: GLA improves cognitive impairment in mice with cerebral ischemia by down-regulating oxidative stress and inhibiting NLRP3-mediated pyroptosis. 2024 Vol. 30 (6): 931-936 [Abstract] ( 23 ) HTML (1 KB)  PDF (2522 KB)  ( 9 ) 936 Analysis of Risk Factors for Severe Low Anterior Resection Syndrome After Low Anterior Resection for Rectal Cancer CHEN Chao, ZHENG Xin, XIONG Bing, et al DOI: 10.3969/j.issn.1006-6233.2024.06.011 Objective: To investigate the hazard factors for severe low anterior resection syndrome(LARS) in patients with rectal cancer after low anterior resection. Methods: A total of 156 patients who underwent low anterior resection for rectal cancer at the Affiliated Hospital of Chengde Medical College from February 2019 to May 2021 were selected as the study subjects. The patients were evaluated postoperatively by telephone follow-up and outpatient visits, and the LARS score was calculated. Univariate and multivariate logistic regression analyses were used to explore the factors influencing the occurrence of severe LARS. Results: Among the 156 patients,69 cases (44.23%) developed LARS, including 34 cases of mild LARS (21.79%) and 35 cases of severe LARS (22.44%). Univariate analysis indicated that neoadjuvant radiotherapy, obesity, height of anastomosis to tooth line(≤2cm), and stomal leak after operation were closely related to severe LARS. Multivariate regression analysis showed that preoperative neoadjuvant radiotherapy, anastomosis-to-dentate line height less than 2cm, BMI ≥ 25kg/m2, and postoperative anastomotic leakage were closely related to the occurrence of severe LARS.Conclusion: Patients with low anterior resection have a higher risk of LARS. Obesity, neoadjuvant radiotherapy, anastomosis distance less than 2cm, and postoperative anastomotic leakage are independent risk factors for severe LARS. 2024 Vol. 30 (6): 936-941 [Abstract] ( 19 ) HTML (1 KB)  PDF (1347 KB)  ( 11 ) 942 Evaluation of the Auxiliary Diagnostic Value of IFN-α IL-6 and IL-17 for Tuberculosis LIU Zhandi, SUN Mingyue, TIAN Erpeng, et al DOI: 10.3969/j.issn.1006-6233.2024.06.012 Objective: To detect and analyze the specificity and sensitivity of serum immune cytokines in mycobacterium tuberculosis (TB) lung disease, and to evaluate its diagnostic value. Methods: A total of 139 patients with suspected pulmonary tuberculosis who were treated in Hebei Chest Hospital from Nov. 2020 to Oct. 2022 were retrospectively selected as the research subjects. According to the latest diagnostic criteria for tuberculosis implemented in 2018 (WS288-2017), 69 cases of confirmed tuberculosis were included in the tuberculosis group, and 70 cases of non-tuberculosis were included in the non-tuberculosis group. The serum levels of immune-related cytokines IL-6, IL-17, and IFN-α were detected by flow immunofluorescence assay, and the diagnostic efficacy of the three factors in tuberculosis was analyzed. Results: The levels of IL-6 and IL-17 in patients with tuberculosis were significantly higher than those in patients without tuberculosis (P<0.05). The concentration of IFN-α in the TB group was slightly higher than that in the non-TB group, There's no statistically significant difference (P>0.05). ROC curve analysis showed that the area under the curve (AUC) of IL-6, IL-17, and IFN-α detection was 0.658, 0.734and 0.571, respectively (P < 0.001). Conclusion: The detection of IL-6 and IL-17 has certain clinical value in the auxiliary diagnosis of tuberculosis. 2024 Vol. 30 (6): 942-945 [Abstract] ( 27 ) HTML (1 KB)  PDF (1240 KB)  ( 23 ) 945 The Value of miR-200c and PAPP-A Levels Combined with Transvaginal Ultrasound in Differentiating Threatened Abortion and Ectopic Pregnancy GUO Caiping, CHEN Yanmei, MA Yanling DOI: 10.3969/j.issn.1006-6233.2024.06.013 Objective: To explore the value of serum miR-200c and pregnancy-associated plasma protein-A (PAPP-A) levels in combination with transvaginal ultrasound for the differential diagnosis of threatened abortion and ectopic pregnancy.Methods: This study was conducted from January 2020 to January 2022, including 43 cases of normal early pregnancy, 43 cases of ectopic pregnancy, and 43 cases of threatened abortion who visited our hospital during this period. All patients underwent transvaginal ultrasound examination, and various ultrasound parameters were recorded. The differences in serum miR-200c and PAPP-A levels at the time of consultation were compared among the groups to investigate their diagnostic value for threatened abortion and ectopic pregnancy.Results: There was no statistically significant difference in the average diameter of the corpus luteum among the three groups (P>0.05). The endometrial thickness in the ectopic pregnancy group was lower than that in the threatened abortion and normal early pregnancy groups, and the thickness in the threatened abortion group was lower than that in the normal early pregnancy group (P<0.05). The resistance index (RI) in the threatened abortion group was higher than that in the ectopic pregnancy and normal early pregnancy groups, and the RI in the ectopic pregnancy group was higher than that in the normal early pregnancy group (P<0.05). The peak systolic velocity (PSV) in both the threatened abortion and ectopic pregnancy groups was lower than that in the normal early pregnancy group (P<0.05), but there was no statistically significant difference between the threatened abortion and ectopic pregnancy groups (P>0.05). The serum miR-200c levels in the threatened abortion and ectopic pregnancy groups were higher than those in the normal early pregnancy group (P<0.05), but there was no statistically significant difference between the threatened abortion and ectopic pregnancy groups (P>0.05). The PAPP-A levels in the ectopic pregnancy group were lower than those in the threatened abortion and normal early pregnancy groups, and the levels in the threatened abortion group were lower than those in the normal early pregnancy group (P<0.05). Analysis showed that the specificity of combined ultrasound, miR-200c, and PAPP-A levels for diagnosing threatened abortion was higher than that of any single indicator, and the specificity and positive predictive value for diagnosing ectopic pregnancy were higher than those of any single indicator.Conclusion: The combination of serum miR-200c and PAPP-A levels with transvaginal ultrasound examination can effectively differentiate between threatened abortion and ectopic pregnancy, improving diagnostic accuracy and specificity, with significant clinical application potential. 2024 Vol. 30 (6): 945-951 [Abstract] ( 22 ) HTML (1 KB)  PDF (1224 KB)  ( 12 ) 951 Clinical Observation of MRI in Diagnosing Rotator Cuff Injuries and Assessing the Degree of Tears PAN Xiaowen, GAO Yan, DAN Qian, et al DOI: 10.3969/j.issn.1006-6233.2024.06.014 Objective: To explore the application efficacy of MRI in diagnosing rotator cuff injury and assessing the tear degree.Methods: A retrospective analysis was conducted on the data of 183 patients with shoulder injuries in our hospital. All patients underwent a magnetic resonance imaging (MRI) examination, using arthroscopy as the gold standard to investigate the diagnostic value of MRI for rotator cuff injuries. The detection of injuries and the degree of tears in different tendons of the rotator cuff were examined, and the deviation in the measurements of tendon tear sizes in full-thickness tears by MRI compared to arthroscopy was analyzed.Results: Arthroscopic examination revealed 136 positive cases of rotator cuff injuries out of 183 patients, and 47 negative cases. MRI detected 134 positive cases and 49 negative cases, with a diagnostic sensitivity of 95.59% (130/136), specificity of 91.49% (43/47), accuracy of 94.54% (173/183), positive predictive value of 97.01% (130/134), negative predictive value of 87.76% (43/49), and a Kappa value of 0.859. Arthroscopy diagnosed a total of 373 injuries in 183 patients, including 136 supraspinatus tendon injuries, 122 infraspinatus tendon injuries, and 115 subscapularis tendon injuries. The detection rate of MRI for supraspinatus, infraspinatus, and subscapularis tendon injuries was significantly lower than that of arthroscopy (P<0.05). Among the 136 patients with rotator cuff injuries, there were 45 cases of full-thickness tears and 91 cases of partial tears. The accuracy of MRI in diagnosing full-thickness tears was 93.33% (42/45) and 94.51% (86/91) for partial tears, with a total accuracy of 94.12% (128/136), which was significantly lower than that of arthroscopy (P<0.05). The accuracy of MRI in measuring the retraction degree and tear span of the tendon ends in full-thickness tears was 86.67% (39/45) and 82.22% (37/45), respectively.Conclusion: MRI has a high diagnostic value for both rotator cuff injuries and the degree of tendon tears, providing a reliable preoperative diagnostic basis for rotator cuff injuries, and is worth promoting in clinical application. 2024 Vol. 30 (6): 951-955 [Abstract] ( 27 ) HTML (1 KB)  PDF (1369 KB)  ( 11 ) 955 Tremor Analysis and External Anal Sphincter Electromyography in Patients with Parkinson's Disease and P-Type Multiple System Atrophy LI Hailiang, CHENG Yanbo, DONG Ruiguo, et al DOI: 10.3969/j.issn.1006-6233.2024.06.015 Objective: To investigate the differences in tremor analysis and external anal sphincter electromyography(EAS-EMG) in Parkinson's disease (PD)and P-type Multiple system Atrophy patients ( MSA-P).Methods: Fifty PD patients and twenty-five MSA-P patients treated at the Department of Neurology, Affiliated Hospital of Xuzhou Medical University, were selected. According to the H&Y staging, PD patients were divided into early-middle stage PD group (H&Y 0-3, 27 cases) and late-stage PD group (H&Y 4~5, 23 cases), along with the MSA-P group (25 cases). Tremor analysis and EAS-EMG were performed for all three groups, and the differences in various indices of tremor analysis and EAS-EMG among the three groups were analyzed.Results: The incidence of resting tremor was higher in the PD group compared to the MSA-P group (P<0.05), with no significant differences in tremor frequency and postural tremor incidence. In the early-middle stage PD group, both resting tremor and postural tremor frequencies were higher than in the late-stage PD group (P<0.05), with resting tremor being predominant in both groups. However, there were no statistically significant differences in the incidence of resting tremor and postural tremor between the two groups (P>0.05). The incidence of resting tremor was higher in the early-middle stage PD group compared to the MSA-P group (P<0.05), with no significant difference in the incidence of postural tremor. Compared with the PD group, the MSA-P group had a prolonged average duration of motion unit of the anal sphincter during slight contraction (MUAP), a significantly higher percentage of polyphasic waves, and a higher incidence of satellite potentials (P<0.05). In the late-stage PD group compared to the early-middle-stage PD group, there was a prolonged average duration of MUAP, a significantly higher percentage of polyphasic waves, and a higher incidence of satellite potentials (P<0.05). Compared to the MSA-P group, the early-middle stage PD group had a shorter average duration of MUAP (P<0.05), a lower percentage of polyphasic waves, and a lower incidence of satellite potentials (P<0.05).Conclusion: Tremor analysis and EAS-EMG are associated with the progression of Parkinson's disease and may provide a basis for differentiating between MSA-P and early-middle-stage PD patients. 2024 Vol. 30 (6): 955-959 [Abstract] ( 21 ) HTML (1 KB)  PDF (1183 KB)  ( 8 ) 960 Clinical and Imaging Risk Factors for Rupture of Multiple Intracranial Aneurysms GUO Ran, XIN Ruiqiang, et al DOI: 10.3969/j.issn.1006-6233.2024.06.016 Objective: To investigate the potential clinical and imaging risk factors for the rupture of multiple intracranial aneurysms.Methods: A retrospective analysis of the clinical and imaging data of patients with multiple intracranial aneurysms treated in our hospital from April 2013 to December 2021 was conducted. Patients were divided into rupture and non-rupture groups based on whether their aneurysms had ruptured. Univariate analysis was performed on the clinical and imaging parameters of both groups to identify statistically significant risk factors, which were then used as independent variables for further multivariate Logistic regression analysis to explore the risk factors influencing the rupture of multiple intracranial aneurysms.Results: Univariate regression analysis indicated significant differences between the rupture group (n=52) and the non-rupture group (n=85) in terms of age, gender, hypertension, hyperlipidemia, atherosclerotic diseases, maximum aneurysm diameter, width, aspect ratio, and aneurysm location (all P<0.05). Younger age was associated with a higher likelihood of rupture (P<0.05). The rupture group had significantly greater maximum diameter, width, and aspect ratio than the non-rupture group (P<0.05). Aneurysms located in the anterior communicating artery, posterior communicating artery, middle cerebral artery, and basilar artery had higher rupture rates compared to aneurysms in other locations (P<0.05). Multivariate Logistic regression analysis identified gender (OR=3.664, 95%CI): 1.577~8.515, P=0.003), maximum aneurysm diameter (OR=1.405, 95% CI: 1.130~1.747, P=0.002), and aneurysm location as independent risk factors for the rupture of multiple intracranial aneurysms. Specifically, aneurysms in the anterior communicating artery (OR=26.747, 95% CI: 5.384~132.883, P<0.001) and posterior communicating artery (OR=15.288, 95% CI: 3.659~63.885, P<0.001) had the highest risk of rupture. Hyperlipidemia was identified as an independent protective factor against the rupture of multiple intracranial aneurysms (OR=0.103, 95% CI: 0.026~0.405, P=0.001). Conclusion: Gender, maximum aneurysm diameter, and aneurysm location are independent risk factors for the rupture of multiple intracranial aneurysms, while hyperlipidemia is an independent protective factor. These findings provide crucial evidence for the development of personalized preventive strategies for unruptured aneurysms in patients with multiple intracranial aneurysms. 2024 Vol. 30 (6): 960-964 [Abstract] ( 35 ) HTML (1 KB)  PDF (1203 KB)  ( 12 ) 964 Effectiveness of Different TAPP Procedures for Treating Giant Inguinal Hernias and Their Impact on Stress Response and Postoperative Complications HAN Hua, LI Zhou, LU Tong DOI: 10.3969/j.issn.1006-6233.2024.06.017 Objective: To analyze the stress response and complication situation in patients with inguinal hernia treated with a transabdominal preperitoneal prosthesis (TAPP) laparoscopic surgery.Methods: The clinical data of 72 patients with inguinal hernia who underwent TAPP surgery in our hospital from October 2021 to December 2022 were analyzed. The patients were randomly divided into Group A (36 cases) and Group B (36 cases). Both groups underwent TAPP surgery, but in Group A, the residual hernia sac was sutured and fixed after dissection and transection during surgery. In contrast, the control group did not undergo this treatment. The postoperative stress response and the incidence of complications in the two groups were observed.Results: The intraoperative blood loss in Group A was significantly higher than in Group B (P<0.05). The incidence of postoperative seroma in Group A was significantly lower than in Group B (P<0.05). The postoperative N and NLR values in both groups were significantly higher than the preoperative values; LY was significantly lower than the preoperative values. The differences in preoperative and postoperative N and NLR were significantly lower in Group A than in Group B (P<0.05), and the difference in LY was significantly higher in Group A than in Group B (P<0.05).Conclusion: In patients with inguinal hernia undergoing TAPP surgery, suturing and fixing the residual hernia sac during the procedure helps reduce the incidence of postoperative seroma, alleviates the body's stress response, and aids in subsequent recovery. 2024 Vol. 30 (6): 964-969 [Abstract] ( 23 ) HTML (1 KB)  PDF (1197 KB)  ( 16 ) 969 Imaging Manifestations of Cervical Cancer on TVCDS and Its Correlation with Tumor Characteristics YANG Liying, WANG Li, LIANG Xiaojing, et al DOI: 10.3969/j.issn.1006-6233.2024.06.018 Objective: To explore the imaging manifestations of cervical cancer on TVCDS (Transvaginal Color Doppler Sonography) and analyze their correlation with tumor characteristics.Methods: A retrospective analysis was conducted on the clinical data of 98 patients with cervical cancer (cervical cancer group) admitted to our hospital from December 2018 to November 2023. Concurrently, 71 subjects with normal TVCDS results during physical examinations were included as a control group. Imaging data from TVCDS examinations of both groups were collected. Comparisons were made between the groups regarding blood flow signal grading, pathological characteristics, RI (Resistance Index), PSV (Peak Systolic Velocity), and EDV (End-Diastolic Velocity). Spearman rank correlation analysis was used to assess the relationship between RI, PSV, EDV, and blood flow signal grading in patients with cervical cancer.Results: The RI value in the cervical cancer group was lower than that in the control group, while PSV and EDV values were higher (P<0.05). Among cervical cancer patients at different clinical stages, RI values were compared as follows: Stage I > Stage Ⅱ > Stage Ⅲ. In contrast, PSV and EDV values were compared as follows: Stage I < Stage Ⅱ < Stage Ⅲ. Patients with tumor diameter < 40mm and no lymph node metastasis had significantly higher RI values than those with tumor diameter ≥ 40mm and lymph node metastasis (P<0.05). Comparisons of RI values among patients with different blood flow signal grades were as follows: Grade I > Grade Ⅱ > Grade Ⅲ. PSV and EDV values were compared as follows: Grade I < Grade Ⅱ < Grade Ⅲ (P<0.05). Correlation results indicated that RI was negatively correlated with blood flow signal grading (r=-0.411, P<0.001), while PSV and EDV were positively correlated with blood flow signal grading (r=0.396, 0.408, P<0.001).Conclusion: Changes in RI, PSV, and EDV values on TVCDS in patients with cervical cancer are closely related to their tumor pathological characteristics and blood flow signal grading. 2024 Vol. 30 (6): 969-973 [Abstract] ( 21 ) HTML (1 KB)  PDF (1465 KB)  ( 15 ) 973 Efficacy of Laparoscopic Minimally Invasive Radical Surgery for Esophageal Cancer via Different Approaches and Its Impact on Postoperative Residual Gastrointestinal Function and Nutritional Status ZHANG Lianfu, JIA Zongxiao, HE Mingyue, et al DOI: 10.3969/j.issn.1006-6233.2024.06.019 Objective: To investigate the efficacy of laparoscopic minimally invasive radical surgery for esophageal cancer via different approaches and its influence on postoperative residual gastrointestinal function and nutritional status.Methods: A total of 126 patients with esophageal cancer admitted to our hospital from May 2020 to May 2023 were selected and randomly divided into two groups: the transabdominal group and the thoracoabdominal group, with 63 cases in each group. The transabdominal group underwent laparoscopic minimally invasive radical surgery, while the thoracoabdominal group underwent thoracoabdominal combined laparoscopic minimally invasive radical surgery. The efficacy, perioperative conditions, trauma indicators [pre- and postoperative levels of C-reactive protein (CRP), interleukin-6 (IL-6), interleukin-8 (IL-8)], gastrointestinal function indicators [postoperative gastric tube removal time, anal exhaust time, eating time, pre- and postoperative levels of vasoactive intestinal peptide (VIP), somatostatin (SS), motilin (MTL)], nutritional status [pre- and postoperative serum albumin (ALB), prognostic nutritional index (PNI), subjective global assessment (SGA) score], and postoperative complications were compared between the two groups.Results: The comparison of positive margin rate, surgical cure rate between the two groups showed no statistical significance (P>0.05); the thoracoabdominal group had shorter operative time (131.52±18.77) min, postoperative anal exhaust time (2.52±0.60) d, postoperative nasogastric tube removal time (2.88±0.47) d, postoperative oral intake time (4.38±0.85) d, postoperative ambulation time (5.82±1.16) d, and hospital stay (10.72±2.01) d, all shorter than those of the abdominal group [(155.94±21.67) min, (3.40±0.66) d, (3.34±0.53) d, (5.62±0.92) d, (7.10±1.24) d, (12.39±2.33) d, respectively], intraoperative blood loss, and postoperative drainage volume were (112.46±14.21) mL and (160.89±22.37) mL, respectively, both lower than those of the abdominal group [(124.31±16.08) mL, (186.27±20.15) mL, respectively], the number of lymph nodes dissected was (15.27±1.76), higher than that of the abdominal group (13.15±1.63) (t=6.761, 7.831, 5.154, 7.858, 5.983, 4.308, 4.383, 6.691, 7.015, all P<0.001); on postoperative days 1 and 3, serum CRP levels of the thoracoabdominal group were (72.36±16.20) mg/L and (35.28±10.08) mg/L, respectively, lower than those of the abdominal group [(96.58±14.77) mg/L and (51.17±11.62) mg/L, respectively], IL-6 levels were (212.34±33.16) ng/L and (142.32±23.57) ng/L, respectively, lower than those of the abdominal group [(254.39±37.49) ng/L and (180.67±27.13) ng/L, respectively], IL-8 levels were (0.28±0.09) μg/L and (0.14±0.04) μg/L, respectively, lower than those of the abdominal group [(0.42±0.11) μg/L and (0.25±0.07) μg/L, respectively], VIP levels were (28.76±4.05) pg/mL and (34.69±4.28) pg/mL, respectively, higher than those of the abdominal group [(25.14±3.63) pg/mL and (30.52±3.91) pg/mL, respectively], SS levels were (18.23±3.10) pg/mL and (20.21±2.36) pg/mL, respectively, higher than those of the abdominal group [(16.17±2.86) pg/mL and (18.08±2.44) pg/mL, respectively], MTL levels were (242.16±21.18) pg/mL and (257.90±23.25) pg/mL, respectively, higher than those of the abdominal group [(227.43±20.64) pg/mL and (238.41±21.76) pg/mL, respectively] (t=8.769, 8.199, 6.668, 8.470, 7.818, 10.829, 5.283, 5.709, 3.877, 4.980, 3.953, 4.858, all P<0.001); 1 month postoperatively, serum ALB and the difference values of the thoracoabdominal group were (75.61±8.03) g/L and (11.95±6.51) g/L, respectively, higher than those of the abdominal group [(70.58±5.95) g/L and (70.58±5.95) g/L, respectively], PNI and the difference values were (62.48±6.17) and (14.22±5.13) g, respectively, higher than those of the abdominal group [(57.13±5.82) and (8.60±5.22), respectively], SGA scores and the difference values were (9.08±1.42) points and (5.49±1.58) points, respectively, higher than those of the abdominal group [(11.15±1.76) points and (3.06±1.49) points, respectively] (t=3.944, 4.8470, 5.007, 6.095, 7.265, 8.881, all P<0.001); the incidence of complications in the thoracoabdominal group was 6.35%, lower than that in the abdominal group of 19.05% (χ2=4.582, P=0.032). Conclusion: Thoracoabdominal combined laparoscopic minimally invasive radical surgery for esophageal cancer is effective, can optimize the surgical pathway, reduce tissue trauma, help restore gastrointestinal function, improve patient nutritional status, reduce the incidence of complications, and accelerate postoperative recovery. 2024 Vol. 30 (6): 973-980 [Abstract] ( 21 ) HTML (1 KB)  PDF (1458 KB)  ( 12 ) 980 Study on the Correlation between CT Pulmonary Artery Imaging and the Severity and Short-term Prognosis of Acute Pulmonary Embolism HU Jie, ZHENG Tongtong, et al DOI: 10.3969/j.issn.1006-6233.2024.06.020 Objective: To evaluate the relationship between CT parameters of pulmonary artery and cardiac function and 30-day adverse prognosis in patients with acute pulmonary embolism, and to provide information for rapid risk stratification in patients with acute pulmonary embolism.Methods: A total of 243 patients who were diagnosed with acute pulmonary embolism by CTPA examination in our hospital and followed up for 30 days were retrospectively analyzed in this study. The ratio of the main pulmonary artery (MPA) to the aorta (AO), the maximum diameter of the left (LPA) and right (RPA) pulmonary artery, and the maximum short diameter ratio between the right and left ventricles (RVD/LVD) were measured on the transverse axis image, and the height and volume of the pulmonary trunk were measured at the workstation. The patients were grouped according to 30-day prognosis, the severity of pulmonary embolism, and embolism type, and various parameters were compared.Results: There were statistically significant differences between the two groups in terms of surgery or immobilization history, heart rate, pulmonary embolism type, and treatment methods (P<0.05); The LPA and RVD/LVD values in the poor prognosis group were greater than those in the good prognosis group (P<0.05); In the grouping based on the location and severity of the embolism, there were statistically significant differences between the two groups in terms of MPA/AO, MPA, RPA, LPA, PAV, PAOI, RVD/LVD, interventricular septal shift, and vena cava reflux (P<0.05); The ratio of right ventricular to left ventricular diameters (RVD/LVD) had the highest AUC value and specificity for predicting moderate to severe pulmonary embolism.Conclusion: The ratio of right ventricular to left ventricular diameters (RVD/LVD) can be used as the primary indicator for daily assessment of the prognosis of acute pulmonary embolism. The volume of the pulmonary artery trunk (PAV) can serve as a new indicator for determining the severity of acute pulmonary embolism. Additionally, MPA, RPA, LPA, vena cava reflux, and interventricular septal shift have certain predictive values for the severity of pulmonary embolism, providing a more reliable basis for clinical personalized treatment plans. 2024 Vol. 30 (6): 980-987 [Abstract] ( 26 ) HTML (1 KB)  PDF (1845 KB)  ( 24 ) 987 Study on the Assessment of Fetal Growth Restriction and Pregnancy Outcome by Color Doppler Ultrasound Combined with Serum Pregnancy-Associated Plasma Protein A SUN Tingting, CHEN Yun, LIU Xianxiang, et al DOI: 10.3969/j.issn.1006-6233.2024.06.021 Objective: To explore the value of color Doppler ultrasound combined with serum pregnancy-associated protein A (PAPP-A) on evaluating fetal growth restriction (FGR) and pregnancy outcomes.Methods: The clinical data of 1465 pregnant women in the first trimester who were admitted to our hospital were retrospectively analyzed from May 2021 to May 2023. All patients underwent color Doppler ultrasound examination after enrollment, and serum PAPP-A was detected. According to the status of FGR, the patients were divided into restricted group (n=105) and normal group (n=1360). The ultrasound examination results and serum PAPP-A level were compared between the two groups. ROC curve was used to analyze the predictive efficiency of ultrasound examination and serum PAPP-A level at admission on FGR. By means of the pregnancy outcomes, the pregnant women in growth restriction group were classified into abnormal pregnancy outcome subgroup and normal delivery subgroup, and the ultrasound results and serum PAPP-A level were compared between the two subgroups. ROC curve was applied to analyze the predictive efficiency of ultrasound examination and serum PAPP-A level at admission on pregnancy outcomes.Results: The UtA-PI and UtA-RI in restricted group were significantly higher than those in normal group while serum PAPP-A level was significantly lower than that in normal group (P<0.05). ROC curve analysis showed that the AUCs of UtA-PI, UtA-RI and serum PAPP-A for predicting FGR were 0.759, 0.631 and 0.855 respectively (P<0.05). The UtA-PI and UtA-RI in abnormal pregnancy outcome subgroup were significantly higher while the level of serum PAPP-A was significantly lower compared to normal delivery subgroup (P<0.05). ROC curve analysis revealed that the AUCs of UtA-PI, UtA-RI and serum PAPP-A were 0.712, 0.692 and 0.658 respectively in predicting abnormal pregnancy outcomes (P<0.05).Conclusion: UtA-PI, UtA-RI values, and serum PAPP-A are closely related to the occurrence of fetal FGR and have high clinical value on predicting the pregnancy outcomes of pregnant women. 2024 Vol. 30 (6): 987-991 [Abstract] ( 28 ) HTML (1 KB)  PDF (1481 KB)  ( 318 ) 992 Changes in the Fractional Amplitude of Low-Frequency Fluctuations in Patients with Cerebellar Infarction on Different Sides and Its Association with Cognitive Function Changes HUANG Qinbin, FENG Bing, CHEN Xiaoqi, et al DOI: 10.3969/j.issn.1006-6233.2024.06.022 Objective: To investigate the changes in the fractional amplitude of low-frequency fluctuations (fALFF) values in different brain regions of patients with cerebellar infarction on different sides and its association with changes in cognitive function.Methods: A retrospective study was conducted on 92 patients with acute unilateral cerebellar infarction diagnosed in the Department of Neurology of our hospital from April 2022 to March 2024. Patients were divided into left-side group (45 cases) and right-side group (47 cases) based on the location of the infarction. An additional 50 healthy individuals were selected as the healthy control group. Cognitive function scores were compared between different groups, and differences in fALFF values between the left-side and right-side groups were analyzed, along with the correlation between fALFF values in different brain regions and cognitive function.Results: The MMSE scores, RAVLT scores, and BNT scores of patients in the cerebellar infarction group were significantly lower than those in the healthy control group (P<0.05), and the TMT time was significantly prolonged compared to the control group (P<0.05). The fALFF values of the right cingulate gyrus, right inferior frontal gyrus, left superior frontal gyrus, and cerebellar posterior lobe in the cerebellar infarction group were significantly higher compared to the healthy control group (P<0.05). In the left-side group, the fALFF values of the right cingulate gyrus, inferior frontal gyrus, and left superior frontal gyrus were significantly higher than those in the right-side group (P<0.05), while the fALFF value of the cerebellar posterior lobe was significantly lower compared to the right-side group (P<0.05). In the right-side group, the fALFF value of the right frontal lobe was positively correlated with MMSE scores (P<0.05), and the right inferior parietal lobule was negatively correlated with MMSE scores (P<0.05). The left anterior cingulate gyrus was positively correlated with RAVLT scores (P<0.05), and the left parietal lobe was negatively correlated with TMT time (P<0.05). In the left-side group, the right prefrontal lobe, inferior parietal lobule, and cingulate gyrus were negatively correlated with MMSE scores (P<0.05), the right inferior parietal lobule was negatively correlated with RAVLT scores (P<0.05), the right occipital lobe was positively correlated with RAVLT scores (P<0.05), and the right parietal lobe was positively correlated with TMT time (P<0.05).Conclusion: Compared to healthy individuals, cognitive function in patients with cerebellar infarction is significantly reduced. At the same time, the fALFF values in key brain regions on the contralateral side of the infarction show significant compensatory increases, which are strongly correlated with cognitive function levels. 2024 Vol. 30 (6): 992-997 [Abstract] ( 18 ) HTML (1 KB)  PDF (1187 KB)  ( 12 ) 997 Efficacy and High-Risk Factors of Cement Leakage in Unilateral and Bilateral Pedicle Approach PVP for Osteoporotic Fractures of the Upper Thoracic Vertebrae GUO Yifan, CHEN Bo, et al DOI: 10.3969/j.issn.1006-6233.2024.06.023 Objective: To analyze the therapeutic effect of unilateral and bilateral pedicle approach percutaneous vertebroplasty (PVP) on patients with upper thoracic osteoporotic fractures, and to explore the high-risk factors affecting bone cement leakage.Methods: A retrospective analysis was conducted on 151 patients with osteoporotic fractures of the upper thoracic vertebrae who underwent PVP surgery in our hospital from June 2021 to June 2023. Among them, 80 patients with a unilateral pedicle approach were designated as the unilateral group, and 71 patients with a bilateral pedicle approach were designated as the bilateral group. The surgery time, blood loss, and intraoperative cement leakage rate were compared between the two groups, along with the pain levels 7 days post-operation. Based on the occurrence of intraoperative cement leakage, patients were divided into the leakage group and the non-leakage group. Logistic multivariate analysis was used to identify high-risk factors for cement leakage.Results: Compared with the bilateral group, the unilateral group had shorter surgery time, less blood loss, and a lower rate of intraoperative cement leakage (P<0.05). The pain level in the unilateral group 7 days post-operation was significantly lower than in the bilateral group (P<0.05). Statistically significant differences were observed between the leakage and non-leakage groups in terms of age, cement dispersion type, spinal kyphosis Cobb angle, and endplate/posterior wall integrity (P<0.05). Logistic multivariate analysis revealed that age ≥70 years, dispersive type of cement dispersion, high spinal kyphosis Cobb angle, and incomplete endplate/posterior wall were high-risk factors for intraoperative cement leakage in PVP (P<0.05).Conclusion: The unilateral pedicle approach PVP surgery shows more significant therapeutic effects compared to the bilateral approach. Various factors influence cement leakage during PVP surgery, and clinical practice should include a comprehensive preoperative evaluation of patients' conditions and optimization measures to reduce the incidence of cement leakage. 2024 Vol. 30 (6): 997-1002 [Abstract] ( 26 ) HTML (1 KB)  PDF (1184 KB)  ( 8 ) 1003 Predictive Value of Serum Cholinesterase Levels Combined with Inflammatory Response Factors for Pulmonary Infection Complicating Severe Acute Pancreatitis FAN Bin, LI QingHe, LI Wei, et al DOI: 10.3969/j.issn.1006-6233.2024.06.024 Objective: To explore the predictive value of serum cholinesterase (ChE), C-reactive protein (CRP), and interleukin-6 (IL-6) for pulmonary infection (PI) in patients with severe acute pancreatitis (SAP).Methods: Eighty SAP patients treated at our hospital from January 2018 to January 2024 were selected as the SAP group. Based on whether these patients developed PI, they were divided into the PI group (40 cases) and the non-PI group (40 cases). According to clinical PI scores, the subjects were categorized into mild, moderate, and severe groups, and 60 healthy individuals were selected as controls. Serum ChE, CRP, and IL-6 levels were measured using ELISA. The pulmonary ventilation function index (mechanical ventilation time (days)) was compared among groups. Logistic regression analysis was used to identify risk factors for PI in SAP patients. The predictive value of serum ChE, CRP, and IL-6 for PI in SAP was assessed using ROC curves.Results: Compared with the control group, the SAP group had significantly lower serum ChE levels and significantly higher CRP and IL-6 levels (P<0.05). Among patients with different PI scores, serum ChE levels showed a pattern of mild > moderate > severe, whereas CRP and IL-6 levels exhibited the opposite trend, with statistically significant differences (P<0.05). The PI group had significantly higher CRP and IL-6 levels and significantly lower serum ChE levels than the non-PI group (P<0.05). The mechanical ventilation time was shorter in the PI group compared to the non-PI group (P<0.05). Logistic regression analysis revealed that increased CRP and IL-6 levels and low serum ChE were independently associated with poor prognosis in SAP patients with PI (P<0.05), with an AUC of 0.807, which was greater than the AUC values of serum ChE, CRP, and IL-6 alone (0.716, 0.749, and 0.732, respectively), all statistically significant (P<0.05). Combined detection had a sensitivity of 87.5% and a specificity of 67.5% for predicting PI in SAP patients.Conclusion: In patients with SAP complicated by PI, decreased serum ChE levels and increased CRP and IL-6 levels are independent risk factors for the occurrence of PI. Combined detection of these three markers has high predictive value for the occurrence of PI in SAP patients. 2024 Vol. 30 (6): 1003-1007 [Abstract] ( 34 ) HTML (1 KB)  PDF (1327 KB)  ( 10 ) 1007 Study on the Effects of Mirror Neuron Rehabilitation Strategy Combined with Transcranial Magnetic Stimulation on Neurological and Functional Recovery Post-Stroke FAN Baiyan, ZHU Jianwen, ZUO Huirong, et al DOI: 10.3969/j.issn.1006-6233.2024.06.025 Objective: To study the effects of the Mirror Neuron System (MNS) rehabilitation strategy combined with Transcranial Magnetic Stimulation (TMS) on neurological and functional recovery post-stroke.Methods: A total of 198 patients with motor dysfunction following a stroke, treated at a military rehabilitation center from January 2019 to June 2023, were selected and randomly divided into three groups of 66 patients each. All three groups received conventional rehabilitation guidance; the TMS group received TMS treatment, the MNS group received MNS treatment, and the combined group received TMS+MNS treatment. Neurological function (NIHSS), upper and lower limb motor function (FMA), activities of daily living (ADL), balance ability (BBS), upper and lower limb muscle tone (MAS), step length difference between healthy and affected sides, percentage of weight-bearing time on the affected side, and language function were compared among the three groups before the intervention, after 2 weeks of intervention, and after 4 weeks of intervention.Results: After 2 and 4 weeks of intervention, the NIHSS scores of the combined group were lower than those of the TMS group and the MNS group, with statistically significant differences (P<0.05); there was no statistically significant difference between the TMS group and the MNS group (P>0.05). After 2 and 4 weeks of intervention, the FMA-upper limb and FMA-lower limb scores of the combined group were higher than those of the TMS group and the MNS group, and the MNS group scored higher than the TMS group, with statistically significant differences (P<0.05). After 2 and 4 weeks of intervention, the ADL scores and BBS scores of the combined group were higher than those of the TMS group and the MNS group, and the MNS group scored higher than the TMS group, with statistically significant differences (P<0.05). After 4 weeks of intervention, the proportion of patients with upper and lower limb MAS grades 0-1 in the combined group was higher than in the TMS group and the MNS group, and higher in the TMS group than in the MNS group, with statistically significant differences (P<0.05). After 2 and 4 weeks of intervention, the step length difference between the healthy and affected sides in the combined group was lower than that in the TMS group and the MNS group, and the percentage of weight-bearing time on the affected side was higher than that in the TMS group and the MNS group, with greater changes observed in the MNS group compared to the TMS group, with statistically significant differences (P<0.05). After 2 and 4 weeks of intervention, scores for reading comprehension, repetition, fluency, and naming in language function were higher in the combined group than in the TMS group and the MNS group, and higher in the TMS group than in the MNS group, with statistically significant differences (P<0.05).Conclusion: MNS combined with TMS can promote neurological and functional recovery post-stroke, and improve patients' activities of daily living, and each method has its advantages and complements the other. The combined treatment is significantly effective and worthy of clinical promotion. 2024 Vol. 30 (6): 1007-1014 [Abstract] ( 21 ) HTML (1 KB)  PDF (1238 KB)  ( 16 ) 1014 Efficacy Observation of Total Hip Arthroplasty Based on AI Three-Dimensional Reconstruction Technology in Adults with Hip Dysplasia GUO Xinyu, HU Wei, PAN Feng, et al DOI: 10.3969/j.issn.1006-6233.2024.06.026 Objective: To explore the effectiveness of total hip arthroplasty (THA) based on artificial intelligence (AI) three-dimensional reconstruction technology in treating adult patients with hip dysplasia (DDH).Methods: A retrospective analysis was conducted on 112 adult DDH patients in our hospital from April 2021 to February 2023. The patients were grouped according to different surgical protocols. The control group (55 cases) underwent THA based on two-dimensional X-ray technology, while the observation group (57 cases) underwent THA based on AI three-dimensional reconstruction technology. Surgical-related indicators, intraoperative prosthesis matching with preoperative planning, radiographic measurement parameters, preoperative and postoperative (1 day and 3 days) inflammation-oxidative stress indicators [interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), superoxide dismutase (SOD), malondialdehyde (MDA)], the excellent and good rate of hip joint function, and the incidence of complications were compared between the two groups.Results: The observation group had shorter operation time, postoperative ambulation time, and hospital stay, and less intraoperative blood loss compared to the control group (P<0.05). The observation group had a higher matching rate of intraoperative acetabular cup and femoral stem models with preoperative planning and a higher excellent and good rate of hip joint function compared to the control group (P<0.05). On postoperative day 1 and day 3, the observation group had lower serum levels of IL-8, TNF-α, and MDA, and higher levels of SOD compared to the control group (P<0.05). The difference in leg length discrepancy between the two groups was lower in the observation group (P<0.05). The overall incidence of complications between the two groups was not statistically significant (P>0.05).Conclusion: THA assisted by AI three-dimensional reconstruction technology in treating adult DDH patients can optimize surgical procedures, reduce traumatic stress, promote early postoperative recovery, improve prosthesis accuracy, reduce leg length discrepancy, and improve hip joint function with good safety. 2024 Vol. 30 (6): 1014-1020 [Abstract] ( 30 ) HTML (1 KB)  PDF (1545 KB)  ( 21 ) 1020 The Efficacy of ECG P-Wave Dispersion Combined with QTc Interval in Predicting Early Recurrence After Radiofrequency Ablation for Paroxysmal Atrial Fibrillation YIN Wei, et al DOI: 10.3969/j.issn.1006-6233.2024.06.027 Objective: To investigate the efficacy of electrocardiogram (ECG) P-wave dispersion (Pd) combined with QTc interval in predicting early recurrence after radiofrequency ablation (RFA) for paroxysmal atrial fibrillation (PAF).Methods: A total of 108 PAF patients who underwent RFA from January 2019 to June 2023 were selected and followed up for 3 months after the operation. They were divided into a recurrence group (28 cases) and a non-recurrence group (80 cases) based on recurrence status. Baseline data, preoperative and postoperative 7-day Pd, QTc, and the differences (△Pd, △QTc) between preoperative and postoperative 7-day Pd, QTc were compared between the two groups. The influence of Pd and QTc on early postoperative recurrence and their predictive efficacy were analyzed.Results: The disease course was longer, and the proportion of hypertension and CHA2DS2-VASc scores were higher in the recurrence group compared to the non-recurrence group (P<0.05). Preoperative and postoperative 7-day Pd (32.68±5.75) ms, (26.27±7.13) ms were higher than those in the non-recurrence group (28.51±5.04) ms, (17.16±6.28) ms, and QTc (458.27±52.31) ms, (410.65±30.52) ms were longer than those in the non-recurrence group (430.19±39.62) ms, (372.06±25.40) ms (t=3.631, 6.376, 2.960, 6.558, all P<0.001). Preoperative and postoperative 7-day △Pd (6.41±2.67) ms, △QTc (47.62±10.33) ms were lower in the recurrence group than in the non-recurrence group (11.35±4.19) ms, (58.13±13.27) ms (t=5.828, 3.803, all P<0.001). Preoperative Pd, QTc were positively correlated with CHA2DS2-VASc score (P<0.05). After adjusting for other factors such as disease course, hypertension, and CHA2DS2-VASc score, △Pd and △QTc remained independent influencing factors for early recurrence after PAF RFA (P<0.05). The AUC for △Pd in predicting early recurrence after PAF RFA was 0.779 (95% CI: 0.689-0.853), with a Youden index of 0.473, sensitivity of 78.57%, and specificity of 68.75%; for △QTc, the AUC was 0.715 (95% CI: 0.620-0.798), with a Youden index of 0.411, sensitivity of 78.57%, and specificity of 62.50%. The combined prediction of △Pd and △QTc for early recurrence after PAF RFA had an AUC of 0.940 (95% CI: 0.878-0.977), Youden index of 0.779, sensitivity of 92.86%, and specificity of 85.00%, which was superior to either predictor alone.Conclusion: The changes in ECG Pd and QTc interval before and after RFA in PAF patients have high efficacy in predicting postoperative recurrence and can provide relevant guidance for clinical prevention and treatment. 2024 Vol. 30 (6): 1020-1025 [Abstract] ( 28 ) HTML (1 KB)  PDF (1369 KB)  ( 18 ) 1026 Assessment Value of Proportion of Low-Density Distribution Area in RR Scatterplot Hcy and FAR for Evaluation of Disease Severity and Prognosis in Patients with Myocardial Infarction QIAN Hua, TAN Mingyue, CHEN Shuping, et al DOI: 10.3969/j.issn.1006-6233.2024.06.028 Objective: To analyze the evaluation value of the proportion of low-density distribution area in RR scatterplot (L), homocysteine (Hcy), and fibrinogen/albumin ratio (FAR) for disease severity and prognosis of myocardial infarction.Methods: A total of 220 patients with myocardial infarction admitted to our hospital from June 2020 to June 2022 were divided into low-grade group (grade Ⅰ~Ⅱ, n=135) and high-grade group (grade Ⅲ~Ⅳ, n=85) according to the Fillip classification standard at admission, and were divided into survival group (n=184) and death group (n=36) according to the discharge prognosis. L, Hcy, and FAR levels were measured among all study subjects at admission. Binary Logistic regression analysis was used to construct a prediction model with L, Hcy and FAR as variables, and the evaluated value of L, Hcy and FAR on severity and prognosis of myocardial infarction patients was analyzed by receiver characteristic operating curve (ROC).Results: The levels of L, Hcy, and FAR in the high-grade group were higher than those in the low-grade group (P<0.05); the levels of L, Hcy, and FAR in the death group were higher than those in the survival group (P<0.05). Logistic regression analysis showed that L, Hcy, and FAR were independent predictive indicators for disease severity and prognosis of myocardial infarction, with predictive models as follows: Y=-12.864+0.065×L+0.250×Hcy+0.892×FAR; Y=-13.367+0.435×Hcy+0.914×FAR. ROC curve analysis showed that the area under the curve (AUC) of combined evaluation of myocardial infarction severity by L, Hcy, and FAR was 0.897, with sensitivity of 95.29% and specificity of 78.52%, which was higher than that of each index evaluated alone (P<0.05); the AUC of combined evaluation of prognosis of myocardial infarction patients by L, Hcy, and FAR was 0.859, with sensitivity of 80.56% and specificity of 81.52%, which was higher than that of each index evaluated alone (P<0.05).Conclusion: L, Hcy, and FAR have certain evaluation value for disease severity and prognosis of myocardial infarction patients, and the efficacy of combined evaluation of the three indicators is significantly improved compared with each index evaluated alone. 2024 Vol. 30 (6): 1026-1031 [Abstract] ( 19 ) HTML (1 KB)  PDF (1517 KB)  ( 11 ) 1031 Study on the Therapeutic Efficacy and Serum Tumor Markers Levels of Patients with Ultrasound-guided Interventional Therapy for Ovarian Endometrioid Cyst ZHANG Rui, REN Yongfeng, XU Yanlan, et al DOI: 10.3969/j.issn.1006-6233.2024.06.029 Objective: To investigate the efficacy and serum tumor markers levels of patients with ultrasound-guided interventional therapy for ovarian endometrioid cyst. Methods: A total of 80 patients with ovarian endometrioid cyst admitted to our hospital from May 2019 to May 2022 were randomly divided into two groups: 40 cases in the control group underwent laparoscopic cystectomy, and 40 cases in the study group underwent ultrasound-guided interventional therapy. The clinical efficacy, operation time, antibiotic use time, and hospital stay were compared between the two groups. The serum tumor marker levels and ovarian function indexes were evaluated before and after the operation. The recurrence rate of the two groups was observed one year after operation. Results: The clinical efficacy of the study group was higher than that of the control group (P<0.05). The operation time, antibiotic usage time, and hospitalization time of the study group were shorter than those of the control group (P<0.05). 6 months after the operation, the serum Carbohydra Antigen 125 (CA125),Carbohydra Antigen 199 (CA199) and Human epididymal protein 4 (HE4) levels in the study group were lower than those in the control group (P<0.05). Six months after the operation, estrogen (E2) level in the study group was lower than that in the control group, Follicle Stimulating Hormones (FSH) and Luteinizing Hormones (LH) levels in the study group were higher than those in the control group (P<0.05). The recurrence rate in the study group was lower than that in the control group 1 year after operation (P<0.05). Conclusion: The use of ultrasound-guided invasive puncture in the treatment of patients with adenometrioid cysts of the ovaries can enhance the efficacy and reduce the level of serum tumor markers, improve the function of the ovaries, and reduce the rate of postoperative relapse. 2024 Vol. 30 (6): 1031-1035 [Abstract] ( 25 ) HTML (1 KB)  PDF (1181 KB)  ( 14 ) 1035 The Impact of Two Fixation Methods on Postoperative Recovery Complications and Cost-effectiveness of Traumatic Distal Radius and Ulna Fractures SHEN Linhua DOI: 10.3969/j.issn.1006-6233.2024.06.030 Objective: To explore the application value of intramedullary nailing and plate fixation in the treatment of traumatic distal radius and ulna fractures through postoperative recovery, complications, and cost-effectiveness.Methods: Ninety-two patients with traumatic distal radius and ulna fractures treated in our hospital from April 2021 to March 2023 were randomly divided into two groups: the intramedullary nailing group (n=46) and the plate group (n=46). The intramedullary nailing group underwent internal fixation with interlocking intramedullary nails, while the plate group underwent internal fixation with plates. General conditions of the two groups, fracture healing process, perioperative pain level, and inflammatory stress [C-reactive protein (CRP), interleukin (IL-1β, IL-18), tumor necrosis factor-alpha (TNF-α)] were observed. Functional recovery rate, complications, and cost-effectiveness were assessed using Flynn's functional recovery scoring criteria at 6 months postoperatively.Results: The intramedullary nailing group had shorter operation time, incision length, and hospital stay [(74.85±12.47) min, (5.02±1.53) cm, (8.60±2.77) d] compared to the plate group [(86.18±14.00) min, (9.77±1.94) cm, (14.18±4.00) d]. The blood loss and drainage volume [(38.59±10.14) ml, (90.03±20.63) ml] were lower than those in the plate group [(120.83±15.67) ml, (151.66±17.94) ml] . The intramedullary nailing group had earlier fracture healing time and full weight-bearing time [(8.56±1.49) weeks, (11.29±3.44) weeks] compared to the plate group [(10.08±1.95) weeks, (13.58±4.36) weeks]. The bone density ratio at the fracture end at 6 months postoperatively was higher in the intramedullary nailing group (0.89±0.06) than in the plate group (0.86±0.05). The VAS pain scores at 6 h, 12 h, 48 h, and 72 h postoperatively were lower in the intramedullary nailing group [(3.26±0.84), (2.87±0.75), (2.50±0.69), (2.48±0.51)] compared to the plate group [(4.00±0.59), (3.74±0.66), (3.52±0.70), (3.36±0.64)] . The CRP levels at 6 h and 72 h postoperatively were lower in the intramedullary nailing group [(15.11±6.00) mg/L, (8.53±1.76) mg/L] compared to the plate group [(18.42±7.03) mg/L, (11.40±2.83) mg/L]. IL-1β, IL-18, and TNF-α levels were also lower in the intramedullary nailing group compared to the plate group (all P<0.05). There was no significant difference in the functional recovery rate between the two groups (P>0.05). The cost-effectiveness of intramedullary nailing was superior to plate fixation.Conclusion: Intramedullary nailing for traumatic distal radius and ulna fractures can optimize surgical procedures, reduce intraoperative bleeding, alleviate perioperative pain, promote postoperative recovery, alleviate patient financial burden, and has good long-term efficacy and safety. 2024 Vol. 30 (6): 1035-1040 [Abstract] ( 31 ) HTML (1 KB)  PDF (1206 KB)  ( 11 ) 1040 Analysis of the Short-Term Efficacy and Adverse Reactions of Albumin-Bound Paclitaxel in the Treatment of Cervical Squamous Cell Carcinoma WU Jiachun, ZHU Ying, ZOU Meiying, et al DOI: 10.3969/j.issn.1006-6233.2024.06.031 Objective: To investigate the short-term efficacy and adverse reactions of albumin-bound paclitaxel in the treatment of cervical squamous cell carcinoma.Methods: From October 2019 to October 2022, 124 patients with cervical squamous cell carcinoma treated at our hospital were selected as subjects and randomly divided into two groups (observation group with 62 cases and control group with 62 cases). Both groups received radiotherapy; the control group was treated with paclitaxel combined with cisplatin, while the observation group was given albumin-bound paclitaxel combined with cisplatin. Clinical efficacy and occurrence of toxic side effects during treatment were recorded after four cycles. Changes in serum tumor markers [squamous cell carcinoma antigen (SCCA), carcinoembryonic antigen (CEA), carbohydrate antigen (CA125)] and T lymphocyte levels (CD3+, CD4+, CD8+) before and after four cycles of treatment were compared between the two groups.Results: After four cycles of treatment, the observation group had a higher treatment efficacy rate (P<0.05). There was no statistically significant difference in the occurrence of toxic side effects between the two groups during treatment (P>0.05). Serum levels of SCCA, CEA, and CA125 decreased in both groups after four cycles of treatment, with a more significant decrease in the observation group (P<0.05). Serum levels of CD3+ and CD4+ decreased in both groups, with a more significant decrease in the control group, while CD8+ levels increased, with a more significant increase in the control group (P<0.05).Conclusion: Albumin-bound paclitaxel is significantly effective in treating cervical squamous cell carcinoma, can effectively control the progression of the disease, and has high safety, making it suitable for clinical promotion. 2024 Vol. 30 (6): 1040-1044 [Abstract] ( 20 ) HTML (1 KB)  PDF (1180 KB)  ( 13 ) 1044 Application of Esketamine in Patients with Traumatic Severe Brain Injury and Analysis of Changes in Serum Neurofactor Levels SHI Dongdong, LIAO Xingzhi, CHEN Tian, et al DOI: 10.3969/j.issn.1006-6233.2024.06.032 Objective: To explore the application of ketamine in patients with traumatic severe craniocerebral injury and analyze the changes in serum neurotrophic factor levels. Methods: A total of 180 patients with traumatic severe craniocerebral injury treated in our hospital from January 2020 to January 2023 were selected as the research objects and divided into two groups, with 90 cases in each group, according to the random number table method. The control group received single sufentanil drug treatment after surgery, while the study group received ketamine treatment based on the control group. The pain score, agitation score, cognitive function (MoCA scale), and changes in serum neurotrophic factors (BDNF, NGF) and inflammatory factors (hs-CRP, TNF-α, IL-6) at different time points were observed in both groups.Results: At 12h and 24h after administration, the pain scores and agitation scores in both groups were lower than before administration, and the scores in the study group were lower than those in the control group at 12h and 24h after administration. The MoCA scores in both groups decreased after administration, with the study group higher than the control group. Both BDNF and NGF levels increased after administration in both groups, with the study group higher than the control group. The levels of hs-CRP, TNF-α, and IL-6 decreased in both groups after administration, with the study group lower than the control group (P<0.05).Conclusion: The application of ketamine in patients with traumatic severe craniocerebral injury can effectively reduce pain and agitation scores, improve cognitive function, promote the generation of neurotrophic factors, and reduce the level of inflammatory factors. 2024 Vol. 30 (6): 1044-1048 [Abstract] ( 29 ) HTML (1 KB)  PDF (1490 KB)  ( 7 ) 1049 Effects of Es-ketamine Combined with Propofol Anesthesia on Stress Levels Cognitive Function, and Serum BDNF and NSE Levels in Elderly Patients Undergoing Radical Mastectomy for Breast Cancer PENG Changsheng, ZHOU Jianjun, HU Xuhua, et al DOI: 10.3969/j.issn.1006-6233.2024.06.033 Objective: To investigate the effects of es-ketamine combined with propofol anesthesia on stress levels, cognitive function, and serum BDNF and NSE levels in elderly patients undergoing radical mastectomy for breast cancer.Methods: Ninety elderly patients who underwent radical mastectomy for breast cancer at our hospital from May 2020 to July 2022 were selected and further divided into the observation group (45 cases, es-ketamine combined with propofol) and the control group (45 cases, propofol anesthesia) according to a random number table method. The stress levels, cognitive function, and serum BDNF and NSE levels of the two groups were compared.Results: In the observation group, cortisol and adrenaline levels at T2, T3, and T4 time points were significantly higher compared to T0 time point (P<0.05). In the control group, cortisol and adrenaline levels at T1, T2, T3, and T4 time points were significantly higher compared to T0 time point (P<0.05). The increase in cortisol and adrenaline levels at T1, T2, T3, and T4 time points in the observation group was significantly lower than that in the control group (P<0.05). Both groups showed a decrease in cognitive function scores and BDNF levels and an increase in NSE levels at 24h and 48h postoperatively, with the observation group showing better changes than the control group (P<0.05). The awakening, extubation, and orientation recovery times in the observation group were significantly shorter than those in the control group (P<0.05).Conclusion: Es-ketamine combined with propofol anesthesia in elderly patients undergoing radical mastectomy for breast cancer can reduce the body's stress response, accelerate cognitive function recovery, and improve the quality of postoperative awakening. 2024 Vol. 30 (6): 1049-1053 [Abstract] ( 33 ) HTML (1 KB)  PDF (1179 KB)  ( 20 ) 1053 Clinical Observation on Therapeutic Efficacy of Peiyuanjianpi Acupuncture and Medicine Prescription on Type 2 Diabetes Mellitus Complicated with Obesity ZHU Hairui, LIU Jia DOI: 10.3969/j.issn.1006-6233.2024.06.034 Objective: To observe the clinical effect of acupuncture and medicine combined with "Gubenpeiyuan, jianpihewei" in the treatment of obese type 2 diabetes.Methods: 80 obese patients with type 2 diabetes mellitus from March 2019 to March 2022 were selected and divided into 2 groups with 40 patients in each group according to the random number table method. The control group was given metformin hydrochloride tablets combined with miglitol tablets. Based on the control group, the treatment group was added with needle and drug combination therapy. Both groups were treated for 30 days (1 course). The differences of relevant observation indexes before and after treatment were compared between the two groups.Results: The total effective rate of the treatment group was higher than that of the control group (P<0.05). There were significant differences in BMI, FPG, FINS, HOMA-IR, TC, TG, and LDL-C between the two groups after treatment compared with before treatment (P<0.05), and the treatment group was superior to the control group (P<0.05). There was no obvious statistical difference in reducing WHR and IAI (P>0.05), indicating that there was no remarkable difference in the reduction of WHR and IAI between the treatment group and the control group.Conclusion: Acupuncture and medicine combined with "Gubenpeiyuan, jianpihewei" has definite clinical efficacy in the treatment of obesity-type 2 diabetes mellitus. It is effective in reducing body weight, reducing fat, restoring blood glucose level, and treating insulin resistance, especially in improving FINS, HDL-C, and reducing TC and LDL-C. The effect of reducing BMI, FPG, TG and HOMA-IR is worthy of affirmation, without side effects, and worthy of clinical application. 2024 Vol. 30 (6): 1053-1056 [Abstract] ( 22 ) HTML (1 KB)  PDF (1174 KB)  ( 17 )

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