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2024 Vol. 30, No. 5
Published: 2024-05-31
705
Effects of Butorphanol on Proliferation Migration and Angiogenesis of Lung Cancer Cells by Regulating the Wnt/β-Catenin Signaling Pathway
WEN Zhu, et al
DOI: 10.3969/j.issn.1006-6233.2024.05.01
Objective:
To investigate the effects of buprenorphine on the proliferation, migration, and angiogenesis of lung cancer cells by regulating the Wnt/β-catenin signaling pathway.
Methods:
Human lung cancer H1299 cells were divided into the control group, low-dose and high-dose buprenorphine groups, high-dose buprenorphine + LiCl (Wnt/β-catenin signaling pathway activator) group, and FH535 group (Wnt/β-catenin signaling pathway inhibitor). The colony formation assay was used to detect the colony formation ability of cells; the Transwell assay was used to detect cell migration and invasion; flow cytometry was used to detect the cell apoptosis rate; the vascular mimicry formation assay was used to observe the angiogenesis; and Western blot was used to detect the expression of VEGF-A, VE-cadherin, Bcl-2, Bax, MMP-9, β-catenin, c-Myc, and cyclin D1 proteins.
Results:
The lumen structure of H1299 cells in the control group was intact; compared with the control group, the clone formation rate of H1299 cells, the numbers of cell migration and invasion, number of lumens, and the expression of VEGF-A, VE cadherin, Bcl-2, MMP-9, β-catenin, c-Myc, and cyclin D1 proteins were greatly reduced in the low-dose butorphanol group, high-dose butorphanol group, and FH535 group, the apoptosis rate and the expression of Bax protein were greatly increased (P<0.05); LiCl was able to partially reverse the inhibitory effect of butorphanol on the malignant biological behavior of H1299 cells (P<0.05); the various detection indicators of H1299 cells in the FH535 group were at the same level as those in the high-dose Butorphanol group (P<0.05).
Conclusion:
Butorphanol can induce apoptosis of lung cancer cells, block migration, proliferation and vascular growth, and then prevent the occurrence and development of lung cancer. The mechanism is related to blocking the activation of Wnt/β-catenin signaling pathway.
2024 Vol. 30 (5): 705-709 [
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710
Effect of miR-1297 on Regulating Invasion and Migration of Gastric Cancer Cells via HMGA1 Gene
WANG Yingxin, MA Yashuai, LI Zhong, et al
DOI: 10.3969/j.issn.1006-6233.2024.05.02
Objective:
To investigate whether miR-1297 regulates the migration and invasion of gastric cancer by targeting high mobility group A1 (HMGA1).
Methods:
The expression levels of miR-1297 and HMGA1 in gastric cancer (n=30) and adjacent normal tissues were determined by RT-qPCR. Gastric adenocarcinoma SGC-7901 cell line and human normal gastric mucosal epithelial cells GES-1 were cultured in vitro. SGC-7901 cells were divided into blank group, negative control group, miR-1297 mimics group, miR-1297 inhibitor and miR-1297 inhibitor+HMGA1 siRNA group. Transwell method was used to detect the migration and invasion of SGC-7901 cells. Western blot tests for changes in protein levels. The specific target genes of miR-1297 were detected by luciferase reporter gene assay.
Results:
The expression of miR-1297 was down-regulated in gastric cancer tissues and SGC-7901 cells, and HMGA1 mRNA level was increased at the same time (P<0.001), and miR-1297 and HMGA1 mRNA expression were negatively correlated (P<0.05). Compared with blank group and negative control group, miR-1297 mimics group could inhibit the migration and invasion ability of cells in vitro (P<0.05). In addition, miR-1297 down-regulates HMGA1 by targeting its 3'-UTR. Compared with blank and negative controls, transfection with miR-1297 inhibitor after downregulation of miR-1297 expression significantly increased cell migration and invasion ability (P<0.05). At the same time, the promoting ability was reversed after transfection of HMGA1 siRNA (miR-1297 inhibitor+HMGA1 siRNA group) (P<0.05).
Conclusion:
miR-1297 inhibits the migration and invasion of SGC-7901 cells by targeting HMGA1, suggesting that miR-1297/HMGA1 axis provides a new prospective therapeutic strategy for gastric cancer.
2024 Vol. 30 (5): 710-714 [
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38
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715
Up-Regulation of LINC01503 Promotes Progression of Laryngeal Squamous Cell Carcinoma
CAO Huan, ZHAO Lei, YANG Jianwang, et al
DOI: 10.3969/j.issn.1006-6233.2024.05.03
Objective:
To investigate the expression of long non-coding RNA (LncRNA) LINC01503 in laryngeal squamous cell carcinoma (LSCC) and its role in promoting cancer progression.
Methods:
The expression level of LINC01503 in LSCC tissues and cells was detected by qRT-PCR, and its correlation with clinical parameters was analyzed. The effects of LINC01503 on the proliferation, migration, and invasion abilities of LSCC cells were assessed using CCK-8 and colony formation assays, Transwell chamber migration and invasion assays, respectively. A nude mouse xenograft model was established to further validate the biological function of LINC01503 in promoting LSCC progression.
Results:
LINC01503 was upregulated in LSCC, and its expression level was associated with clinical stage, tumor differentiation grade, and lymph node metastasis in LSCC patients. In vitro and in vivo experiments demonstrated that overexpression of LINC01503 promoted the proliferation, migration, and invasion abilities of LSCC cells and tumor growth in nude mice, while knockdown of LINC01503 expression led to the opposite effects.
Conclusion:
LINC01503 is upregulated in LSCC and acts as an oncogene to promote the progression of LSCC by enhancing cell proliferation, migration, and invasion.
2024 Vol. 30 (5): 715-719 [
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720
Role of EDEM1 in Promoting Intracranial Aneurysm Development by Stabilizing ATF6
ZHANG Xin, LIANG Wenbao, ZHAO Heng, et al
DOI: 10.3969/j.issn.1006-6233.2024.05.04
Objective:
To investigate the role of endoplasmic reticulum-associated degradation-enhancing α-glucosidase-like protein 1 (EDEM1) and activating transcription factor 6 (ATF6) in the development of intracranial aneurysms (IA).
Methods:
Forty IA patients and 40 healthy subjects were recruited. Serum was collected from both groups, and ATF6 levels were detected by ELISA. IA patients were divided into mild, moderate, and severe disease groups according to Hunt-Hess and Fisher grading. ATF6 levels in the serum of the three groups were compared. si-EDEM1 and si-NC were transfected into HCAECs cells, and the cells were divided into si-EDEM1 and si-NC groups. Western blot was used to detect the expression levels of EDEM1 and ATF6 proteins in the two groups of cells, and CCK-8 assay was used to detect the proliferation of the two groups of cells. Co-immunoprecipitation was used to detect the interaction between EDEM1 and ATF6. si-ATF6 and si-NC were transfected into HCAECs cells, and the cells were divided into si-ATF6 and si-NC groups. Western blot was used to detect the expression levels of EDEM1 and ATF6 proteins in the two groups of cells, and CCK-8 assay was used to detect the proliferation of the two groups of cells.
Results:
Compared with healthy subjects, the serum ATF6 level in IA patients was increased (P<0.05). The ATF6 level increased sequentially in the mild, moderate, and severe disease groups (P<0.05). Compared with the si-NC group, the expression levels of EDEM1 and ATF6 proteins were decreased in the si-EDEM1 group cells, and the proliferation of the cells was decreased (P<0.05). EDEM1 and ATF6 interact with each other. Compared with the si-NC group, the expression level of ATF6 protein was decreased in the si-ATF6 group cells, and the proliferation of the cells was decreased (P<0.05).
Conclusion:
EDEM1 promotes the proliferation of arterial endothelial cells by stabilizing ATF6 and is associated with the progression of IA.
2024 Vol. 30 (5): 720-723 [
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37
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724
Silent HMGB1 Alleviates LPS or IL-17A Induced Inflammation and Apoptosis of Human Middle Ear Epithelial Cells via Inhibiting p38 MAPK Signaling Pathway
ABULAJIANG Tuoheti, ABULIKEMU Yi, UUMAIER Yasheng, et al
DOI: 10.3969/j.issn.1006-6233.2024.05.05
Objective:
To investigate the regulatory effects and mechanisms of high-mobility group protein B1 (HMGB1) on the inflammatory response and apoptosis of human middle ear epithelial cells (HMEECs) induced by lipopolysaccharide (LPS) or interleukin-17A (IL-17A).
Methods:
HMEEC cell line was cultured. HMEECs were divided into the control group, LPS group, LPS+shRNA negative control group (LPS+shNC group), LPS+shRNA-mediated HMGB1 knockdown group (LPS+shHMGB1 group), LPS+p38 mitogen-activated protein kinase (p38 MAPK) inhibitor SB202190 group (LPS+SB202190 group), IL-17A group, IL-17A+shNC group, IL-17A+shHMGB1 group, and IL-17A+SB202190 group. Enzyme-linked immunosorbent assay (ELISA) was used to detect the changes in the levels of TNF-α, IL-1β and IL-6. Western blot was used to detect the expression of mucin 5AC (MUC5AC), mucin 8 (MUC8), p38 MAPK, phosphorylated p38 MAPK (p-p38 MAPK), E-twenty-six like 1 protein (ELK1), B-cell lymphoma 2 protein (Bcl-2), and Bcl-2 associated X protein (Bax). Flow cytometry was used to detect cell apoptosis.
Results:
Compared with the control group, the apoptosis rate of HMEECs in the LPS group or IL-17A group was increased, and the expression levels of HMGB1, MUC5AC, MUC8, TNF-α, IL-1β, IL-6, p38, p-p38, ELK1, and Bax were increased, while the expression level of Bcl-2 was decreased (all P<0.05). Compared with the LPS group or IL-17A group, the apoptosis rate was decreased in the LPS+shHMGB1 group or IL-17A+shHMGB1 group, and the expression levels of HMGB1, MUC5AC, MUC8, TNF-α, IL-1β, IL-6, p38, p-p38, ELK1, and Bax were all decreased, while the expression level of Bcl-2 was increased (all P<0.05). Compared with the LPS group or IL-17A group, the apoptosis rate was decreased in the LPS+SB202190 group or IL-17A+SB202190 group, and the expression levels of MUC5AC, MUC8, TNF-α, IL-1β, IL-6, p38, p-p38, ELK1, and Bax were all decreased, while the expression level of Bcl-2 was increased (all P<0.05).
Conclusion:
Silent HMGB1 alleviates LPS or IL-17A-induced inflammation and apoptosis of HMEECs via inhibiting p38 MAPK signaling pathway.
2024 Vol. 30 (5): 724-730 [
Abstract
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30
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731
PF-127 Hydrogel Combined with Bone Marrow Mesenchymal Stem Cell-Derived Exosome-miR-132 to Induce Osteogenic Differentiation for Repair of Alveolar Bone Defects
QIU Jingyi, YUAN Jing
DOI: 10.3969/j.issn.1006-6233.2024.05.06
Objective:
To investigate the effect of PF-127 hydrogel combined with bone marrow mesenchymal stem cell-derived exosome-miR-132 on the repair of alveolar bone defects by inducing osteogenic differentiation.
Methods:
Bone marrow mesenchymal stem cells (BMSCs) were cultured and transfected with anti-miR-132 and anti-miR-NC plasmids. Bone mesenchymal stem cell-derived exosomes (BMSCs-exo) were prepared and identified for expression of marker proteins by Western blot. PF-127 hydrogel and BMSCs-Exo complexes were prepared and BMSCs uptake was detected by PKH67 labeling; osteogenic ability of BMSCs was detected by alizarin red staining; reverse transcription-polymerase chain reaction (RT-PCR) was used to detect miR-132 expression and mRNA expression of alkaline phosphatase (ALP), osteocalcin (OCN), and Runt-related transcription factor 2 (Runx2) in cells. An alveolar bone defect model was established in rats and the rats were randomly divided into three groups: PF-127 hydrogel group, PF-127 hydrogel+BMSCs-exo-anti-miR-NC group, and PF-127 hydrogel+BMSCs-exo-anti-miR-132 group. Micro-computed tomography (micro-CT) was used to evaluate the alveolar bone defect; Western blot was used to detect the expression of ALP, OCN, and Runx2 proteins in alveolar bone tissue.
Results:
miR-132 expression was significantly lower in the anti-miR-132 group BMSCs than in the anti-miR-NC group (P<0.05), indicating successful transfection of anti-miR-132 into BMSCs. Exosome markers CD9 and CD63 were significantly expressed in the BMSCs-exo-anti-miR-NC and BMSCs-exo-anti-miR-132 groups. Compared with the BMSCs-exo-anti-miR-NC group, miR-132 expression was significantly lower in the BMSCs-exo-anti-miR-132 group (P<0.05). Compared with the PF127 hydrogel group, miR-132 expression was significantly lower in the BMSC-exo-anti-miR-NC group, PF127 hydrogel+BMSC-exo-anti-miR-NC group, and PF127 hydrogel+BMSC-exo-anti-miR-132 groups, and the relative activity of alizarin red staining, Runx2, ALP, and OCN mRNA expression in cells were significantly increased, with the most significant changes in the PF127 hydrogel+BMSC-exo-anti-miR-132 group (P<0.05). Compared with the control group, BV/TV ratio, BMD, Tb.N, Tb.Th, and Runx2, ALP, and OCN protein expression in cells were significantly higher in the PF-127 hydrogel+BMSCs-exo-anti-miR-NC and PF127 hydrogel+BMSCs-exo-anti-miR-132 groups, and Tb.Sp was significantly lower (P<0.05), with the most significant changes in the PF127 hydrogel+BMSCs-exo-anti-miR-132 group.
Conclusion:
PF-127 hydrogel combined with bone marrow mesenchymal stem cell-derived exosome-miR-132 to interfere with miR-132 expression can promote osteogenic differentiation of bone marrow mesenchymal stem cells, thereby promoting the repair of alveolar bone defects in rats.
2024 Vol. 30 (5): 731-737 [
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31
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738
miR-29c-5p Aggravates Ischemic Stroke Reperfusion Injury by Promoting Ferroptosis via Downregulation of LRP6
ZHEN Wenjian, HAO Jinmin, SU Jianlong, et al
DOI: 10.3969/j.issn.1006-6233.2024.05.07
Objective:
To investigate the mechanism of miR-29c-5p in regulating ferroptosis in cerebral ischemia-reperfusion injury.
Methods:
In this study, male SD rats were subjected to middle cerebral artery occlusion (MCAO) surgery and then reperfused. Neurological function scores and TTC staining were used to assess brain injury and infarct volume. qPCR was used to detect the relative expression level of miR-29c-5p. Western blot was used to detect the expression levels of glutathione peroxidase 4 (GPx4) and low-density lipoprotein receptor-related protein 6 (LRP6). The contents of GSH, Fe, and malondialdehyde (MDA) were detected using the corresponding assay kits, and dual luciferase reporter gene assay was used to verify the target gene of miR-29c-5p.
Results:
With the increase of ischemia-reperfusion time in rats, the relative expression level of miR-29c-5p increased and ferroptosis was aggravated. In addition, agomiR-29c-5p intervention also aggravated brain injury and ferroptosis, while antagomiR-29c-5p intervention reversed these effects. Furthermore, combined intervention of agomiR-29c-5p and Fer-1 alleviated brain injury and ferroptosis. The results of dual luciferase reporter gene assay showed that LRP6 is a target gene of miR-29c-5p.
Conclusion:
In this study, miR-29c-5p promotes ferroptosis and aggravates cerebral ischemia-reperfusion injury in rats by downregulating LRP6.
2024 Vol. 30 (5): 738-743 [
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31
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744
X-Chromosome Linked Apoptosis Inhibitor-associated Protein Factor 1 Regulates Caspase 1-mediated Pyroptosis of Gastrointestinal Stromal Tumor Cells via Autophagy Pathway
ZHOU Jianghao, XIE Shuhai, CHEN Yong
DOI: 10.3969/j.issn.1006-6233.2024.05.08
Objective:
To investigate the effect and mechanism of X-chromosome-linked apoptosis inhibitor-associated protein factor 1 (XAF1) on Caspase 1-mediated pyroptosis in human gastrointestinal stromal tumor (GIST) cells.
Methods:
Real-time fluorescent quantitative PCR and western blot were used to detect the expression levels of XAF1 in human normal gastric mucosal epithelial cell line (RGM-1) and GIST cell lines (GIST-T1, GIST-430, GIST-882). pcDNA3.1-XAF1 recombinant plasmid was transfected into GIST-882 cells by liposome-mediated transfection to construct GIST-882 cells with high XAF1 expression. GIST-882 cells were divided into control group, pcDNA3.1 group (transfected with pcDNA3.1 empty vector plasmid), pcDNA3.1-XAF1 group (transfected with pcDNA3.1-XAF1 recombinant plasmid), and pcDNA3.1-XAF1+Rap group (transfected with pcDNA3.1-XAF1 recombinant plasmid and treated with autophagy activator rapamycin). Immunofluorescence staining was used to detect the expression of microtubule-associated protein 1 light chain 3 (LC3) in each group of cells. Western blot was used to detect the LC3Ⅱ/LC3Ⅰ ratio and Beclin1 protein expression level in each group of cells. ELISA was used to detect the contents of IL-1β and IL-18 in the supernatant of each group of cells. Lactate dehydrogenase (LDH) release experiment was used to detect the LDH release rate of each group of cells. Western blot was used to detect the expression levels of Caspase-1 and its activated form cleaved-Caspase-1, NOD-like receptor pyrin domain-containing protein 3 (NLRP3), and gasdermin D (GSDMD) protein in each group of cells.
Results:
Compared with RGM-1 cells, the relative expression levels of XAF1 mRNA and protein were significantly decreased in GIST-T1, GIST-430, and GIST-882 cells (P<0.05). After cell transfection, the relative expression levels of XAF1 mRNA and protein in GIST-882 cells of pcDNA3.1-XAF1 group were significantly higher than those of pcDNA3.1 group and control group (P<0.05). Compared with the control group, the LC3 fluorescence intensity in GIST-882 cells of pcDNA3.1-XAF1 group was significantly weakened, the LC3Ⅱ/LC3Ⅰ ratio and Beclin1 protein relative expression level were significantly decreased (P<0.05), the contents of IL-1β and IL-18 in the cell supernatant and LDH release rate were significantly increased (P<0.05), and the relative expression levels of Caspase-1, cleaved-Caspase-1, NLRP3, and GSDMD proteins were also significantly increased (P<0.05). Compared with the pcDNA3.1-XAF1 group, the LC3 fluorescence intensity in GIST-882 cells of the pcDNA3.1-XAF1+Rap group was significantly enhanced, the LC3Ⅱ/LC3Ⅰ ratio and Beclin1 protein relative expression level were significantly increased (P<0.05), and at the same time, the contents of IL-1β and IL-18 in the cell supernatant and LDH release rate were significantly decreased (P<0.05), and the relative expression levels of Caspase-1, cleaved-Caspase-1, NLRP3, and GSDMD proteins were also significantly decreased (P<0.05).
Conclusion:
XAF1 is downregulated in GIST cells. Overexpression of XAF1 can promote Caspase 1-mediated pyroptosis of GIST cells by inhibiting autophagy and exert anti-tumor effects.
2024 Vol. 30 (5): 744-749 [
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750
Static Magnetic Field Promotes the Proliferation Activity and Migration Phenotype of Skin Fibroblasts through Activation of the FGFR1/JAK2/STAT3 Signaling Pathway
ZHANG Zhihui, MA Juan, YU Yang, et al
DOI: 10.3969/j.issn.1006-6233.2024.05.09
Objective:
To investigate the regulatory effects of static magnetic field (SMF) on the proliferation activity and migration phenotype of skin fibroblasts and its potential mechanism.
Methods:
Human skin fibroblasts (HSFs) were divided into five groups: control group, SMF group, SMF+AG group, SMF+LCK group, and SMF+Stattic group. The control group was HSFs cultured normally. HSFs cells in the SMF group were exposed to 1mT SMF. The other three groups were pre-incubated with inhibitors of FGFR1, JAK2, and STAT3 (3 μmoL/L AG, 3 nmoL/L LCK, and 20 μmoL/L Stattic) together with HSFs and exposed to 1 mT SMF. All groups were treated for 24 hours. Cell Counting Kit-8 (CCK-8) was used to analyze cell proliferation activity. ELISA was used to detect the level of Bcl-2-associated X protein (BAX). Western blot was used to detect the expression of apoptotic marker cleaved-caspase3 and FGFR1, JAK2, STAT3, phosphorylated (p)-FGFR1, p-JAK2, p-STAT3, and the expression of cell migration-related phenotypes high-mobility group box 1 (HMGB1), vimentin, vascular endothelial growth factor A (VEGFA), matrix metallopeptidase-9 (MMP-9), and MMP-2.
Results:
Compared with the control group, the cell proliferation activity in the SMF group was increased, the BAX level was decreased, the protein expression level of cleaved-caspase3 was down-regulated, and the expression levels of p-FGFR1, p-JAK2, p-STAT3, HMGB1, vimentin, VEGFA, MMP-9, and MMP-2 were all significantly up-regulated (all P<0.05). Compared with the SMF group, the cell proliferation activity in the SMF+AG group was decreased, the BAX level was increased, the protein expression level of cleaved-caspase3 was up-regulated, and the expression levels of p-FGFR1, p-JAK2, p-STAT3, HMGB1, vimentin, VEGFA, MMP-9, and MMP-2 were all significantly down-regulated (all P<0.05). Compared with the SMF group, the cell proliferation activity in the SMF+LCK group was decreased, the BAX level was increased, the protein expression level of cleaved-caspase3 was up-regulated, and the expression levels of JAK2, p-JAK2, p-STAT3, HMGB1, vimentin, VEGFA, MMP-9, and MMP-2 were all significantly down-regulated (all P<0.05). Compared with the SMF group, the cell proliferation activity in the SMF+Stattic group was decreased, the BAX level was increased, the protein expression level of cleaved-caspase3 was up-regulated, and the expression levels of STAT3, p-STAT3, HMGB1, vimentin, VEGFA, MMP-9, and MMP-2 were all significantly down-regulated (all P<0.05).
Conclusion:
SMF promotes the proliferation activity and migration phenotype of skin fibroblasts by activating the FGFR1/JAK2/STAT3 signaling pathway.
2024 Vol. 30 (5): 750-755 [
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756
Triptolide Alleviates Hypoxic/Reoxygenation Injury of Cortical Neurons by Inhibiting TLR4/NF-κB Pathway
GUAN Feifei, HAN Zhaoxu, et al
DOI: 10.3969/j.issn.1006-6233.2024.05.010
Objective:
To investigate the effects of Triptolide (TPL) on hypoxia/reoxygenation (H/R) injury of cortical neurons and explore its mechanism based on toll-like receptor 4/nuclear factor-κB (TLR4/NF-κB) signaling pathway.
Methods:
The cortical neurons of SD suckling rats were isolated and cultured in vitro, and the H/R damaged cortical neuron model was prepared by hypoxia for 4h and then reoxygenation for 24h. The normal control group, model group, TPL (25mg/L) group, TPL (25mg/L) +TAK242 (TLR4 inhibitor, 1mg/L) group, TPL (25mg/L)+LPS (TLR4 agonist, 0.1mg/L) group were set up. 24h after administrating separately, the neuronal activity was detected by MTT, the neuronal apoptosis was detected by flow cytometry. The content of inflammatory factors (TNF-α, IL-1β, IL-6) were detected by ELISA. The TLR4/NF-κB signaling pathway related proteins [TLR4, NF-κB p65, p-NF-κB p65, B-lymphoblastoma-2 gene (bcl-2), bcl-2 associated X protein (Bax), Caspase-3, cleaved Caspase-3] were detected by Western blot.
Results:
Compared with the model group, the activity of cortical neuron in TPL group was increased while the apoptosis rate was decreased (P<0.05). The content of TNF-α, IL-1β, IL-6 in culture medium were significantly decreased (P<0.05). The expression of TLR4 and the expression ratio of p-NF-κB p65/NF-κB p65, Bax/bcl-2, cleaved Caspase-3/Caspase-3 were significantly decreased (P<0.05). TAK242 could significantly enhance the regulatory effects of TPL on the activity, apoptosis, inflammatory factors content and the expression of TLR4/NF-κB signaling pathway related protein in H/R damaged cortical neurons. And LPS could significantly reversed the regulatory effects of TPL on H/R damaged cortical neurons.
Conclusion:
TPL can reduce inflammation and apoptosis by inhibiting the activation of TLR4/NF-κB pathway, and thus play a protective role in H/R injury of cortical neurons.
2024 Vol. 30 (5): 756-761 [
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762
VD3 Regulates ROS Mediated TXNIP/NLRP3 Pathway to Inhibit Hyperglycemia-Induced Renal Mesangial Cell Fibrosis
LIU Gang, et al
DOI: 10.3969/j.issn.1006-6233.2024.05.011
Objective:
To investigate the effect of active vitamin D3 (VD3) on the characteristics of renal mesangial cell fibrosis induced by high glucose, and to explore the relevant mechanism.
Methods:
Rat mesangial cell line HBZY-1 was cultured and divided into normal glucose culture (NG) group, normal glucose combined with VD3 culture (NG+VD3) group, high glucose culture (HG) group, high glucose combined with VD3 culture (HG+VD3) group, high glucose combined with n-acetylcysteine culture (HG+NAC) group. The proliferation activity of cells in each group was detected by CCK-8 method, the apoptosis rate of cells in each group was detected by flow cytometry, the content of reactive oxygen species (ROS) in cells in each group was detected by DCFH-DA method, the levels of interleukin-1β (IL-1β), IL-18 and tumor necrosis factor-α (TNF-α) in cell supernatant were detected by enzyme-linked immunosorbent assay (ELISA), the mRNA expression and the protein expression of transforming growth factor-β1 (TGF-β1), fibronectin (FN), intercellular adhesion molecule-1 (ICAM-1), thioredoxin interacting protein (TXNIP), NOD-like receptor heat protein domain associated protein 3 (NLRP3) in cells in each group were detected by real-time fluorescent quantitative polymerase reaction (RT-qPCR) and Western blot.
Results:
Compared with NG group, the proliferative activity in HG group was significantly increased (P<0.05), the apoptosis rate was significantly decreased (P<0.05), ROS content was significantly increased (P<0.05), the levels of IL-1β, IL-18 and TNF-α in cell supernatant were significantly increased (P<0.05), and the mRNA and protein relative expression levels of TGF-β1, FN, ICAM-1, TXNIP and NLRP3 were significantly up-regulated (P<0.05). Compared with HG group, the proliferative activity in HG+VD3 and HG+NAC groups was significantly decreased (P<0.05), the apoptosis rate was significantly increased (P<0.05), the ROS content was significantly decreased (P<0.05), the levels of IL-1β, IL-18 and TNF-α in cell supernatant were significantly decreased (P<0.05), and the mRNA and protein relative expression levels of TGF-β1, FN, ICAM-1, TXNIP and NLRP3 were significantly down-regulated (P<0.05).
Conclusion:
VD3 can alleviate hyperglycemia-induced hyperproliferation and fibrosis of renal mesangial cells, which may be related to the regulation of ROS/TXNIP/NLRP3 pathway.
2024 Vol. 30 (5): 762-767 [
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768
Berberine Regulates Ovalbumin to Induce Th Cell Differentiation in Asthmatic Mice
NI Wenchang, AI Kui, LIU Ting, et al
DOI: 10.3969/j.issn.1006-6233.2024.05.012
Objective:
To investigate the regulation of berberine on Th cell differentiation and Notch signaling pathway in allergic asthmatic-model mice, as well as the repair of lung tissue injury.
Methods:
Mice were induced by OVA, and divided into control group, model group, low-dose berberine group, high-dose berberine group, and dexamethasone group after intervention with low and high doses of berberine and dexamethasone. The general conditions of the mice in each group were observed, HE staining was used to observe the inflammatory infiltration of lung tissue, flow cytometry was used to detect the proportions of Th1, Th2, and Th17 cells in lung tissue suspension, ELISA was used to detect the levels of IFN-γ, IL-4, and IL-17 in bronchoalveolar lavage fluid, and Western blot was used to detect the expression of Notch1 and Hes1 protein in lung tissue.
Results:
Compared with the control group, the mice in the model group had reduced food and water intake, anxiety, mucus in the nose and mouth, obvious inflammation in the lung tissue, decreased Th1 cell proportion and IFN-γ level (P<0.05), increased Th2 and Th17 cell proportions, and IL-4 and IL-17 levels (P<0.05), and increased Notch1 and Hes1 protein expression (P<0.05). Compared with the model group, the mice in the berberine and dexamethasone intervention groups had increased food and water intake, improved mental state, reduced mucus in the nose and mouth, reduced lung tissue inflammation, increased Th1 cell proportion and IFN-γ level (P<0.05), decreased Th2 and Th17 cell proportions, and IL-4 and IL-17 levels (P<0.05), and decreased Notch1 and Hes1 protein expression (P<0.05).
Conclusion:
Berberine intervention can alleviate lung tissue inflammatory damage in allergic asthma model by regulating Th subset differentiation and inhibiting Notch1 protein expression.
2024 Vol. 30 (5): 768-772 [
Abstract
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30
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773
Effect of Combined Ropivacaine and Sufentanil Anesthesia in Mid-Pregnancy on Progeny Brain-Derived Neurotrophic Factor Expression and Cognitive Function in SD Rats
DI Yan, LI Lihan, JIAO Shanshan, et al
DOI: 10.3969/j.issn.1006-6233.2024.05.013
Objective:
To investigate the effects of combined ropivacaine and sufentanil anesthesia in mid-pregnancy on progeny brain-derived neurotrophic factor (BDNF) expression and cognitive function in SD rats.
Methods:
The study hypothesized that maternal treadmill exercise during pregnancy could prevent neurotoxicity induced by ropivacaine and sufentanil. In the first experiment, pregnant rats were exposed to a 100 μL mixture of sufentanil (0.25 μg/μL) and ropivacaine (0.125 μg/μL) (R&G) on gestational (G) day 14, or were exposed continuously on G13, G14, and G15 (R&Gx3). In the second experiment, pregnant rats in the exercise group were forced to run on a treadmill for 60 minutes daily throughout pregnancy. The TrkB antagonist ANA-12 was used to investigate whether the BDNF/TrkB signaling pathway was involved in the neuroprotection provided by maternal exercise.
Results:
Repeated but not single exposure to ropivacaine and sufentanil led to a decrease in histone acetylation and BDNF expression in fetal brain tissue and postnatal hippocampus. Spatial learning and memory impairments were compromised, accompanied by a reduction in dendritic spine number. Maternal exercise mitigated these effects, but the TrkB antagonist ANA-12 abolished the beneficial effects of maternal exercise.
Conclusion:
Multiple (but not single) exposures to ropivacaine and sufentanil in mid-pregnancy in pregnant rats lead to long-term learning and memory impairments in offspring. Maternal exercise improves postnatal neurocognitive deficits by enhancing histone acetylation and activating downstream BDNF/TrkB signaling.
2024 Vol. 30 (5): 773-780 [
Abstract
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30
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781
Protective Effect of Polysaccharide D from Ophiopogon Japonicus on Inflammatory Bowel Disease in Rats by Activating cAMP/PKA/CREB Signaling Pathway
ZHU Shuguang, LI Shuhui, CHEN Peng, et al
DOI: 10.3969/j.issn.1006-6233.2024.05.014
Objective:
To investigate the protective effect of polysaccharide D from Ophiopogon japonicus (MCD) on inflammatory bowel disease (IBD) in rats and its potential mechanism.
Methods:
Male SD rats were randomly divided into 5 groups: control group, model group, MCD low-dose group (50 mg/kg·d), MCD high-dose group (100 mg/kg·d), and MCD+H-89 group (100 mg/kg·d MCD+5 mg/kg·d H-89). The model group was induced by 2,4-dinitrochlorobenzene (DNCB) sensitization and repeated intracolonic administration of DSS. The treatment groups were given MCD or MCD+H-89 for 28 days. Body weight, colon length, serum IL-10, cAMP levels, proportions of Treg cells in peripheral blood, expressions of Bcl-2, p-PKA/PKA, p-CREB/CREB proteins in colon tissues, serum IL-17, IL-6, TNF-α levels, proportions of Th17 cells and Th17/Treg ratios in peripheral blood, and Bax protein expression were determined.
Results:
After treatment, compared with the control group, the model group rats showed significant colon tissue damage, decreased body weight and colon length, decreased serum IL-10 and cAMP levels, decreased proportions of Treg cells in peripheral blood, decreased expressions of Bcl-2, p-PKA/PKA, p-CREB/CREB proteins in colon tissues, increased serum IL-17, IL-6, TNF-α levels, increased proportions of Th17 cells and Th17/Treg ratios in peripheral blood, and increased Bax protein expression (P<0.05). Compared with the model group, the colon tissues of rats in the MCD low-dose and high-dose groups were significantly alleviated, body weight and colon length were increased, serum IL-10 and cAMP levels were increased, proportions of Treg cells in peripheral blood were increased, expressions of Bcl-2, p-PKA/PKA, p-CREB/CREB proteins in colon tissues were increased, serum IL-17, IL-6, TNF-α levels were decreased, proportions of Th17 cells and Th17/Treg ratios in peripheral blood were decreased, and Bax protein expression was decreased (P<0.05). H-89 could partially reverse the therapeutic effect of MCD on IBD rats (P<0.05).
Conclusion:
MCD can reduce the inflammatory response and colon tissue damage in IBD rats, possibly by activating the cAMP/PKA/CREB signaling pathway.
2024 Vol. 30 (5): 781-786 [
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30
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787
Effect of Lung Protective Ventilation Strategies on Respiratory Function and Hemodynamics in Chronic Obstructive Pulmonary Disease (COPD) Rats
XIE Xiang, WANG Yan, CAO Dongming
DOI: 10.3969/j.issn.1006-6233.2024.05.015
Objective:
To investigate the effects of different tidal volumes and positive end-expiratory pressure (PEEP) lung protective ventilation strategies on respiratory function and hemodynamics in COPD rat models.
Methods:
COPD rat models were established by smoke inhalation and intratracheal administration of lipopolysaccharide (LPS). Rats were randomly divided into 9 groups (10 rats per group): control and COPD groups received tracheal intubation only without mechanical ventilation; L+P0 group, L+P3 group, L+P5 group, and L+P10 group received mechanical ventilation with 6 ml/kg tidal volume and 0 cm H
2
O, 3 cm H
2
O, 5 cm H
2
O, and 10 cm H
2
O PEEP, respectively, for 120 min after tracheal intubation; H+P0 group, H+P3 group, H+P5 group, and H+P10 group received mechanical ventilation with 20 ml/kg tidal volume and 0 cm H
2
O, 3 cm H
2
O, 5 cm H
2
O, and 10 cm H
2
O PEEP, respectively, for 120 min after tracheal intubation. Respiratory function and hemodynamic parameters were measured using a small animal non-invasive respiratory function monitoring system and blood gas analyzer. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of inflammatory mediators in lung tissue and bronchoalveolar lavage fluid (BALF).
Results:
Compared with the control group, COPD rats showed significantly decreased respiratory function parameters and PaO
2
(P<0.05); compared with the COPD group, L+P0 group, L+P3 group, and L+P5 group showed significantly increased respiratory function parameters and PaO
2
(P<0.05), while L+P10 group, H+P0 group, H+P3 group, H+P5 group, and H+P10 group showed significantly decreased respiratory function parameters and PaO
2
(P<0.05); L+P5 group showed significantly higher respiratory function parameters and PaO
2
than L+P0 group and L+P3 group (P<0.05); compared with L+P5 group, L+P10 group, H+P0 group, H+P3 group, H+P5 group, and H+P10 group showed significantly decreased respiratory function parameters and PaO
2
(P<0.05). Compared with the control group, COPD rats showed significantly increased levels of IL-8 and TNF-α in lung tissue and BALF, while IL-10 levels were significantly decreased (P<0.05); compared with the COPD group, L+P0 group, L+P3 group, and L+P5 group showed significantly decreased levels of IL-8 and TNF-α and significantly increased levels of IL-10 (P<0.05), while L+P10 group, H+P0 group, H+P3 group, H+P5 group, and H+P10 group showed significantly increased levels of IL-8 and TNF-α and significantly decreased levels of IL-10 (P<0.05); L+P5 group showed significantly lower levels of IL-8 and TNF-α in lung tissue and significantly higher levels of IL-10 than L+P0 group and L+P3 group (P<0.05); compared with L+P5 group, L+P10 group, H+P0 group, H+P3 group, H+P5 group, and H+P10 group showed significantly increased levels of IL-8 and TNF-α and significantly decreased levels of IL-10 (P<0.05).
Conclusion:
Lung protective ventilation strategies with low tidal volume and higher PEEP (such as 5 cm H
2
O PEEP) can alleviate lung inflammation and lung injury in COPD rats, improve respiratory function and hemodynamics in COPD patients.
2024 Vol. 30 (5): 787-793 [
Abstract
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30
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794
Influence of 5-Aminolevulinic Acid Photodynamic Therapy on the Expression of p53 and Caveolin-1 in Bowen's Disease Lesions
ZHANG Yanfeng, FAN Leiqiang, GAO Yue, et al
DOI: 10.3969/j.issn.1006-6233.2024.05.016
Objective:
To investigate the influence and significance of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) on the expression of p53 and Caveolin-1 in Bowen's disease (BD) lesions.
Methods:
Forty cases of Bowen's disease and adjacent normal skin tissues were treated with ALA-PDT. Immunohistochemical staining (SP method) was used to detect the positive expression rates of p53 and Caveolin-1 in BD and surrounding normal skin tissues before and after photodynamic therapy.
Results:
Before treatment, the positive rates of p53 protein expression in normal skin tissue and BD were 10% and 40%, respectively (χ
2
=11.202, P＜0.001), with a statistically significant difference. After treatment, the positive rates of p53 in normal skin tissue and BD were 10% and 5% (χ
2
=0.712, P=0.399), respectively, with no statistically significant difference between them. The difference in the positive expression rate of p53 in BD before and after treatment was statistically significant (χ
2
=14.811, P＜0.001). Before treatment, the positive expression rates of Caveolin-1 in normal skin tissue and BD were 15% and 55%, respectively (χ
2
=14.449, P＜0.001), with a statistically significant difference. After treatment, the positive rates of Caveolin-1 in normal skin tissue and BD were 5% and 12.5% (χ
2
=0.816, P=0.366), respectively, with no statistically significant difference between them. The difference in the positive expression rate of Caveolin-1 in BD before and after treatment was statistically significant (χ
2
=19.013, P＜0.001). There was a positive correlation between p53 and Caveolin-1 expression in BD (r=0.533, P=0.015).
Conclusion:
The high expression of p53 and Caveolin-1 may be closely related to the occurrence of BD, and ALA-PDT can inhibit the expression of p53 and Caveolin-1, thereby inhibiting the progression of the disease. With a larger sample size, p53 and Caveolin-1 may become diagnostic tools for skin-related diseases and provide new targets for their treatment.
2024 Vol. 30 (5): 794-797 [
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30
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798
Association between microRNA-1470 and PATZ1 Expression in Hepatocellular Carcinoma Tissues and Patient Prognosis within 5 Years
TIAN Lihui, ZHANG Zhenyi, YUAN Zengjiang, et al
DOI: 10.3969/j.issn.1006-6233.2024.05.017
Objective:
To investigate the association between the expression levels of microRNA-1470 (miR-1470), POZ domain and AT-binding motif zinc finger protein 1 (PATZ1) in hepatocellular carcinoma (HCC) tissues and patient prognosis within 5 years.
Methods:
A total of 135 patients with HCC who underwent radical resection at Handan Central Hospital from September 2015 to August 2018 were enrolled in this study. HCC tissues and corresponding adjacent normal tissues (normal liver tissues more than 2 cm away from the tumor margin) were collected during surgery. Immunohistochemistry was used to detect PATZ1 protein expression; real-time fluorescence quantitative PCR was used to detect miR-1470 and PATZ1 mRNA expression. Patients were followed up for 5 years, and 5-year overall survival rates were calculated. The expression of miR-1470 and PATZ1 mRNA and protein in HCC tissues and adjacent normal tissues was compared; the relationship between miR-1470 and PATZ1 mRNA expression and clinicopathological parameters, their correlation, and their association with prognosis were analyzed; and the risk factors affecting prognosis were identified. Dual luciferase reporter gene assay was used to verify the target relationship between miR-1470 and PATZ1.
Results:
The positive expression rate of PATZ1 protein in HCC tissues was significantly lower than that in adjacent normal tissues (P<0.05). Compared with adjacent normal tissues, the expression level of miR-1470 was significantly upregulated and the expression level of PATZ1 mRNA was significantly downregulated in HCC tissues (P<0.05). Patients with low miR-1470 and high PATZ1 mRNA expression in HCC tissues had significant differences in serum alpha-fetoprotein (AFP) level, China Liver Cancer Staging System (CNLC) stage, and the presence or absence of vascular tumor thrombus (P<0.05). The expression of miR-1470 and PATZ1 mRNA was negatively correlated in HCC tissues (P<0.05). The 5-year overall survival rates of patients with low miR-1470 expression and high PATZ1 mRNA expression were significantly higher than those of patients with high miR-1470 expression and low PATZ1 mRNA expression (P<0.05). CNLC stage III, vascular tumor thrombus, high miR-1470 expression, and low PATZ1 mRNA expression were independent risk factors for poor prognosis in HCC patients (P<0.05). miR-1470 could negatively regulate PATZ1 expression.
Conclusion:
miR-1470 is upregulated and PATZ1 is downregulated in HCC tissues, and the two are negatively correlated. Both can be used as biomarkers to evaluate the prognosis of HCC patients, and miR-1470 can negatively regulate PATZ1 expression.
2024 Vol. 30 (5): 798-804 [
Abstract
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27
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805
Application of CO
2
Fractional Laser Pretreatment Combined with Photodynamic Therapy in Patients with Facial Flat Warts
JIANG Chongjing, LIU Rongrong
DOI: 10.3969/j.issn.1006-6233.2024.05.018
Objective:
To investigate the efficacy of CO
2
fractional laser pretreatment combined with photodynamic therapy in patients with facial flat warts.
Methods:
A total of 109 patients with facial flat warts who visited our hospital from May 2020 to March 2023 were randomly divided into a control group (55 cases, 2 cases were excluded, and 53 cases were finally completed) and an observation group (54 cases, 1 case was excluded, and 53 cases were finally completed) using a random number table method. The control group was treated with photodynamic therapy alone, and the observation group was treated with photodynamic therapy after CO
2
fractional laser pretreatment. The total effective rate and recurrence rate at 6 months follow-up were compared between the two groups. The changes in inflammatory factors [interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interferon-γ (INF-γ)] and lesion symptom scores before and after treatment were observed in the two groups.
Results:
The overall efficacy of the observation group was higher than that of the control group (P<0.05). There was no difference in inflammatory factors between the two groups before treatment (P>0.05). The inflammatory factors decreased after treatment in both groups, and the difference in inflammatory factors before and after treatment in the observation group was greater than that in the control group (P<0.05). There was no difference in lesion symptom scores between the two groups before treatment. The number, size, color, thickness, itching degree, and isomorphic reaction scores of lesions decreased after treatment in both groups, and the difference in lesion symptom scores before and after treatment in the observation group was greater than that in the control group (P<0.05). After 6 months of follow-up, the recurrence rate in the observation group was 3.701% (1/27 cases) compared with 25.00% (4/16 cases) in the control group, which was not statistically significant (P>0.05).
Conclusion:
CO
2
fractional laser pretreatment combined with photodynamic therapy can reduce inflammatory response, alleviate symptoms, and improve the efficacy in patients with facial flat warts.
2024 Vol. 30 (5): 805-809 [
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34
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810
Analysis of the Relationship between Ultrasound Blood Flow Parameters and Clinical Pathology and Ultrasound TI-RADS Grading of Papillary Thyroid Cancer
ZHENG Wei, ZHENG Hui, CHEN Lu
DOI: 10.3969/j.issn.1006-6233.2024.05.019
Objective:
To analyze the relationship between ultrasound blood flow parameters and clinical pathology and ultrasound TI-RADS grading of papillary thyroid cancer.
Methods:
A total of 103 patients with papillary thyroid cancer diagnosed in our hospital from October 2020 to November 2023 were enrolled in the study. All subjects underwent ultrasound examination to obtain blood flow parameters and B-ultrasound TI-RADS grading. The blood flow parameters of patients with different pathological features and TI-RADS grades were compared, and the correlation between ultrasound blood flow parameters and papillary thyroid cancer pathology and TI-RADS grading was analyzed.
Results:
The pulsatility index (PI), resistance index (RI), and peak systolic velocity to peak diastolic velocity ratio (S/D) in patients with stages Ⅲ~Ⅳ were higher than those in patients with stages Ⅰ~Ⅱ (P<0.05); The PI, RI, and S/D in patients with cervical lymph node metastasis were significantly higher than those in patients without metastasis (P<0.05); The PI, RI, and S/D in patients with TI-RADS grades 5-6 were higher than those in patients with grades 4a-4c (P<0.05). Spearman rank correlation analysis showed that PI, RI, and S/D were positively correlated with TNM stage and TI-RADS grading in patients with papillary thyroid cancer (rTNM stage=0.401, 0.453, 0.462; rTI-RADS grading=0.308, 0.376, 0.368, P<0.05).
Conclusion:
Ultrasound blood flow parameters PI, RI, and S/D are closely related to tumor staging and TI-RADS grading in patients with papillary thyroid cancer. Early measurement of ultrasound blood flow quantitative parameters can provide certain reference information for the clinical diagnosis and prediction of papillary thyroid cancer.
2024 Vol. 30 (5): 810-813 [
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29
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814
Effect of Intermuscular Approach under Quadrant Channel in Treating Lumbar Disc Herniation and Its Influence on Lumbar Function Recovery, Pain Degree and Imaging Parameters
LI Bin, CAI Jinlei, TIAN Jun, et al
DOI: 10.3969/j.issn.1006-6233.2024.05.020
Objective:
To explore the effect of intermuscular approach under Quadrant channel in the treatment of lumbar disc herniation (LDH) and its influence on lumbar function recovery, pain degree and imaging parameters.
Methods:
A total of 102 patients with LDH who were scheduled to undergo transforaminal lumbar interbody fusion (TLIF) in our hospital were enrolled from March 2020 to March 2023, and were divided into observation group (51 cases) and control group (51 cases) by adopting random number table method. The control group was treated with TLIF through traditional open approach, and the observation group was given TLIF through intermuscular approach under Quadrant channel. The surgical conditions of the two groups were compared. MacNab standard was used to evaluate the postoperative rehabilitation effect. Oswestry disability index (ODI) and low back pain numerical rating scale (NRS) were applied to assess the lumbar function recovery and pain degree before surgery and at 1 week, 3 months and 6 months after surgery. The changes in lumbar-pelvic sagittal imaging parameters [lumbar lordosis angle (LL), lumbosacral angle (LSA), pelvic tilt angle (PT), sacral slope (SS) and pelvic incidence angle (PI)] were compared before surgery and at 6 months after surgery, and the complications of the two groups were observed.
Results:
The surgical time and postoperative ambulation time in observation group were shorter (P<0.05), and the intraoperative blood loss and postoperative drainage volume were less than those in control group (P<0.05). At 6 months after surgery, the excellent and good rate of 98.04% was significantly higher than 84.32% in control group (P<0.05). ODI score and lumbar pain NRS score showed statistically significant differences in inter-group effect, time effect, and interaction between grouping and time (P<0.05). Both scores exhibited a decreasing trend over time, with a more pronounced decreasing trend observed in the observation group (P<0.05). At 6 months after surgery, the LL angle significantly decreased compared to the preoperative values in both groups, and the decrease was more pronounced in the observation group than in the control group (P<0.05), but there were no obvious differences in LSA, PT, SS and PI between the two groups (P>0.05). At 6 months after surgery, the total incidence rate of complications was significantly lower in observation group than that in control group (P<0.05).
Conclusion:
Compared with TLIF through traditional open approach, TLIF through intermuscular pproach under Quadrant channel for LDH has smaller trauma, simpler operation and faster recovery of postoperative lumbar function, and it is helpful to further reduce the pain degree and improve the lumbar stability, and it has high safety.
2024 Vol. 30 (5): 814-819 [
Abstract
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30
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22
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820
Analysis of the Efficacy of 3D Technology-Assisted Surgical Treatment for Unstable Pelvic Fractures
SONG Jian, WANG Peiyong, FANG Haolin, et al
DOI: 10.3969/j.issn.1006-6233.2024.05.021
Objective:
To analyze the effectiveness of three-dimensional (3D) technology-assisted surgery in the treatment of unstable pelvic fractures.
Methods:
Eighty patients with pelvic fractures treated in our hospital from March 2020 to September 2022 were selected. All patients met the criteria for Tile B and C unstable fractures. Patients were randomly divided into an observation group (n=40) and a control group (n=40) using a random number table. The control group was treated with traditional surgery, and the observation group was treated with 3D technology-assisted surgery. The two groups were compared in terms of peri-operative related indicators, pelvic function 6 months after surgery, and the incidence of complications within 6 months after surgery.
Results:
The observation group had lower intraoperative bleeding compared to the control group, shorter surgical and suture removal times compared to the control group, and the surgical cost is higher than that of the control group (t=-3.967、-2.194、-2.134、-2.301,P<0.05). Within one week after surgery, imaging in the observation group showed a total satisfaction rate of 95.00% with fracture reduction, which was higher than 80.00% in the control group (χ
2
=4.114,P<0.05). The excellent and good rate of pelvic function in the observation group at 6 months after surgery was 87.50%, higher than the 67.50% in the control group (χ
2
=4.588,P<0.05). There was no statistically significant difference in the total incidence of complications 6 months after surgery between the observation group (7.50%) and the control group (10.00%) (χ
2
=0.157,P>0.05).
Conclusion:
3D technology-assisted surgical treatment for pelvic fractures can shorten the operation time, reduce blood loss, reduce the time for postoperative suture removal, improve the excellent rate of fracture reduction and pelvic function, and achieve remarkable efficacy.
2024 Vol. 30 (5): 820-823 [
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23
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824
Application of CT 3D Reconstruction and Thin-Slice Scanning in Idiopathic Scoliosis Correction Surgery
LUO Xiaoling, LIU Yuqin, LI Lan, et al
DOI: 10.3969/j.issn.1006-6233.2024.05.022
Objective:
To investigate the application of CT 3D reconstruction and thin-slice scanning in idiopathic scoliosis correction surgery.
Methods:
This retrospective study included 112 patients with idiopathic scoliosis who underwent elective correction surgery at our institution between June 2020 and June 2023. Patients were divided into two groups based on surgical approach: the freehand pedicle screw placement group (n=42), who received conventional anatomical landmark guidance with C-arm fluoroscopy, and the CT 3D guidance group (n=70), who received CT 3D reconstruction guidance combined with thin-slice scanning. The two groups were compared in terms of pedicle screw accuracy, intraoperative fluoroscopy count, blood loss, surgical time, and postoperative length of hospital stay. The visual analogue scale (VAS) for pain, Japanese Orthopaedic Association (JOA) score, and Cobb angle changes were recorded and compared pre- and postoperatively for both groups. Complications were also observed.
Results:
A total of 1192 pedicle screws were inserted in the CT 3D guidance group and 673 pedicle screws were inserted in the freehand pedicle screw placement group. The pedicle screw accuracy rate in the CT 3D guidance group was 95.71% (1141/1192), which was significantly higher than that in the freehand pedicle screw placement group [91.08% (613/673); P<0.05]. The CT 3D guidance group had fewer intraoperative fluoroscopies, less blood loss, and shorter surgical time compared to the freehand pedicle screw placement group (P<0.05). There was no significant difference in postoperative length of hospital stay between the two groups (P>0.05). Compared to preoperative values, both groups showed a decrease in VAS scores, an increase in JOA scores, and a decrease in main Cobb angles postoperatively (P<0.05). The CT 3D guidance group had a greater improvement in JOA scores and a greater reduction in main Cobb angles compared to the freehand pedicle screw placement group (P<0.05), while there was no significant difference in VAS score improvement between the two groups (P>0.05). No serious complications occurred in either group, and only one patient developed wound healing complications.
Conclusion:
CT 3D reconstruction and thin-slice scanning can effectively guide idiopathic scoliosis surgery, improving surgical quality, reducing intraoperative X-ray exposure, shortening surgical time, decreasing blood loss, and promoting postoperative recovery.
2024 Vol. 30 (5): 824-828 [
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22
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51
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829
Clinical Analysis of Transurethral Holmium Laser Enucleation of the Prostate in Treating Patients with Recurrent Benign Prostatic Hyperplasia
XU Yanli, WANG Jian, SUN Guoliang
DOI: 10.3969/j.issn.1006-6233.2024.05.023
Objective:
To investigate the clinical effect of transurethral holmium laser enucleation of the prostate (HoLEP) in treating patients with recurrent benign prostatic hyperplasia (BPH).
Methods:
Sixty patients who developed moderate to severe lower urinary tract obstruction symptoms after undergoing transurethral resection of the prostate (TURP) and were treated in the Urology Department of Chengde Central Hospital from September 2020 to September 2023 were selected as the study subjects. The patients were randomly divided into two groups according to a random number table: control group and observation group, with 30 patients in each group. The control group underwent TURP, while the observation group underwent HoLEP. The clinical effectiveness of the two groups was compared.
Results:
Before treatment, there were no significant differences in prostate function and recovery scores between the two groups (P>0.05). After treatment, both groups showed significant improvement (P<0.05), with the observation group showing significantly better results than the control group, with statistically significant differences (P<0.05). The incidence of postoperative complications in the observation group was lower than that in the control group (P<0.05). Additionally, clinical indicators such as operation time, hospital stay, blood loss, and bladder irrigation time in the observation group were better than those in the control group (P<0.05).
Conclusion:
In the surgical treatment of recurrent BPH, transurethral HoLEP not only enhances the effectiveness and safety of the procedure but also reduces the incidence of postoperative complications, making it worthy of clinical promotion.
2024 Vol. 30 (5): 829-833 [
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24
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834
Evaluation of the Value of Full-Automatic Breast Volume Ultrasound in Assessing Benign and Malignant Breast Lesions
ZHAO Ruixin, YANG Jinjun, WANG Ping
DOI: 10.3969/j.issn.1006-6233.2024.05.024
Objective:
To investigate the value of full-automatic breast volume ultrasound (ABVS) in evaluating benign and malignant breast lesions.
Methods:
A retrospective analysis was conducted on the clinical data of 95 patients who were diagnosed with breast lumps in our hospital from September 2020 to September 2023. The patients underwent both conventional ultrasound examination and ABVS after admission. Pathological examination was used as the gold standard, and the diagnostic performance of conventional ultrasound and ABVS was analyzed.
Results:
A total of 124 lesions were found in the 95 patients. Among these, there were 38 malignant lesions and 86 benign lesions. The diagnostic sensitivity, specificity, accuracy, positive predictive value, and negative predictive value of conventional ultrasound were 73.68%, 77.91%, 76.61%, 59.57%, and 87.01%, respectively. The diagnostic sensitivity, specificity, accuracy, positive predictive value, and negative predictive value of ABVS were 84.21%, 88.37%, 87.10%, 76.19%, and 92.68%, respectively. The combined diagnostic sensitivity, specificity, accuracy, positive predictive value, and negative predictive value of conventional ultrasound and ABVS were 71.05%, 95.34%, 87.90%, 87.09%, and 88.17%, respectively.
Conclusion:
Compared with conventional ultrasound, ABVS has a greater advantage in the diagnosis of breast diseases. The combined diagnosis of the two can significantly improve the diagnostic accuracy of benign and malignant breast lesions.
2024 Vol. 30 (5): 834-837 [
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30
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13
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838
Efficacy of HLA-DR/CD4
+
IL-6 and PCT in Predicting Secondary Infection in ICU Patients with Severe Acute Pancreatitis and Guidance for Rational Use of Antimicrobials
GAO Jing, ZHOU Peng, XIE Jing, et al
DOI: 10.3969/j.issn.1006-6233.2024.05.025
Objective:
To investigate the diagnostic efficacy and guidance value for rational use of antimicrobials of human leukocyte antigen-DR/CD4
+
T lymphocytes (HLA-DR/CD4
+
), interleukin-6 (IL-6), and procalcitonin (PCT) in patients with secondary infection of severe acute pancreatitis (SAP) in the ICU.
Methods:
A total of 80 patients with SAP admitted to the ICU of our hospital from May 2020 to December 2023 were enrolled in the study. Patients were divided into an infection group (n=43) and a non-infection group (n=37) according to whether or not they had secondary infection. Clinical data of all patients were collected, including general information, laboratory indicators, and antimicrobial treatment information. The general information and laboratory indicators of the two groups were compared. Logistic regression analysis was used to analyze the independent risk factors for secondary infection in severe SAP, and receiver operating characteristic (ROC) curve analysis was used to evaluate the efficacy of serum HLA-DR/CD4
+
, IL-6, and PCT in predicting secondary infection in SAP.
Results:
The APACHE II score in the infection group was higher than that in the non-infection group (P<0.05). There was no significant difference in gender, age, etiology, and complications (hypertension, type 2 diabetes, and hyperlipidemia) between the two groups (P> 0.05). The levels of lipase, IL-6, and PCT in the infection group were higher than those in the non-infection group (P<0.05), and the level of HLA-DR/CD4
+
was lower than that in the non-infection group (P<0.05). Elevated lipase (OR=2.354, 95% CI 1.491~3.716), elevated HLA-DR/CD4
+
(OR=3.508, 95% CI 1.283~9.588), elevated IL-6 (OR=4.284, 95% CI 1.469~12.493) and elevated PCT (OR=5.743, 95% CI 1.530~21.563) were all independent risk factors for secondary infection in SAP (P<0.05). The AUC values of serum HLA-DR/CD4
+
, IL-6, PCT and the three combined for diagnosing SAP secondary infection were 0.809, 0.778, 0.819 and 0.959, respectively, indicating a certain predictive value. The AUC value and diagnostic efficacy of combined diagnosis were significantly higher than those of single HLA-DR/CD4
+
(z=3.161, P=0.002), IL-6 (z=3.822, P<0.001) and PCT (z=3.346, P=0.001) diagnosis. The level of serum HLA-DR/CD4
+
in the localized infection group was higher than that in the severe infection group, while the levels of IL-6 and PCT were both lower than those in the severe infection group (P<0.05). The duration of antimicrobial use, ICU stay, and total hospital stay in the localized infection group were all significantly shorter than those in the severe infection group (P<0.05).
Conclusion:
Elevated HLA-DR/CD4
+
, IL-6, and PCT are all independent risk factors for secondary infection in SAP and can effectively predict secondary infection in SAP.
2024 Vol. 30 (5): 838-843 [
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844
Analysis of the Correlation between Coagulation and Fibrinolysis Imbalance and Inflammatory Indicators in Patients with Idiopathic Pulmonary Fibrosis and Respiratory Failure
WANG Yingying, GU Dongwei, ZHENG Lei
DOI: 10.3969/j.issn.1006-6233.2024.05.026
Objective:
To investigate the correlation between coagulation and fibrinolysis imbalance and inflammatory indicators in patients with idiopathic pulmonary fibrosis (IPF) and respiratory failure (RF).
Methods:
A total of 98 patients with IPF treated in our hospital from October 2020 to December 2023 were selected and divided into RF group (n=42) and non-RF group (n=56) based on whether they had RF. An automated coagulation analyzer was used to measure activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), and fibrinogen (FIB) levels; D-dimer (D-D) level was measured by immunoturbidimetry; neutrophil percentage (NEUT) and white blood cell count (WBC) were measured by automated blood cell analyzer; C-reactive protein (CRP) level was measured by enzyme-linked immunosorbent assay (ELISA); and procalcitonin (PCT) level was measured by radioimmunoassay. The levels of coagulation and fibrinolysis indicators and inflammatory indicators in the two groups were compared; Pearson correlation analysis was used to analyze the correlation between coagulation and fibrinolysis indicators and inflammatory indicators; and the relationship between coagulation and fibrinolysis indicators and inflammatory indicators and IPF combined with RF was analyzed.
Results:
APTT, TT, and PT were lower in the RF group than in the non-RF group, while FIB and D-D were higher in the RF group than in the non-RF group, and the differences were statistically significant (P<0.05). NEUT, WBC, CRP, and PCT levels were higher in the RF group than in the non-RF group, and the differences were statistically significant (P<0.05). Pearson correlation analysis showed that APTT, TT, and PT were negatively correlated with NEUT, WBC, CRP, and PCT, while FIB and D-D were positively correlated with NEUT, WBC, CRP, and PCT (P<0.05). APTT, TT, PT, FIB, D-D, NEUT, WBC, CRP and PCT were risk factors for IPF combined with RF.
Conclusion:
Patients with IPF combined with RF have coagulation and fibrinolysis imbalance and inflammatory response, and coagulation and fibrinolysis imbalance is closely related to the changes in inflammatory indicators, which is worthy of clinical reference.
2024 Vol. 30 (5): 844-848 [
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26
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849
Correlation of miR-152 and miR-210 in Peripheral Blood of Pregnant Women with the Extent of Pre-eclampsia and Pregnancy Outcome
ZHAO Zeyan, et al
DOI: 10.3969/j.issn.1006-6233.2024.05.027
Objective:
To investigate the correlation between the expression levels of microRNA-152 (miR-152) and miR-210 in peripheral blood of pregnant women and the severity of preeclampsia (PE) and pregnancy outcomes.
Methods:
A total of 200 patients with PE admitted to our hospital from July 2018 to May 2022 were selected. According to the severity of the disease, they were divided into the mild group (mild PE patients, n=114) and the severe group (severe PE patients, n=86). Another 100 healthy pregnant women of the same age were selected as the control group. The blood pressure [systolic blood pressure (SBP), diastolic blood pressure (DBP)], 24-hour urine protein quantitative (24 h Upro), calcium ion (Ca
+
), and peripheral blood miR-152 and miR-210 levels in the three groups were compared. The correlation between peripheral blood miR-152 and miR-210 levels and PE severity, blood pressure, 24 h Upro, and Ca
+
was analyzed. The PE patients were followed up until the end of pregnancy, and the clinical data, peripheral blood miR-152, and miR-210 levels of PE patients with different pregnancy outcomes were compared. Lasso regression and Logistic regression were used to analyze the correlation between the predictive factors and adverse pregnancy outcomes. The receiver operating characteristic curve (ROC) and clinical decision curve (DCA) were used to evaluate the value and clinical utility of peripheral blood miR-152 and miR-210 in predicting adverse pregnancy outcomes in PE patients.
Results:
There were statistically significant differences in SBP, DBP, 24 h Upro, peripheral blood miR-152, and miR-210 levels among the severe group, mild group, and control group (P<0.05). The peripheral blood miR-152 and miR-210 levels were positively correlated with the severity of PE, blood pressure, and 24 h Upro (P<0.05), and negatively correlated with Ca
+
level (P<0.05). The age, abortion history, severity, SBP, DBP, 24 h Upro, Ca
+
, and peripheral blood miR-152 and miR-210 levels of patients with adverse pregnancy outcomes were compared with those of patients without adverse pregnancy outcomes, and the differences were statistically significant (P<0.05). Lasso regression selected five predictive variables: severity, SBP, 24 h Upro, and peripheral blood miR-152 and miR-210 levels. Logistic regression showed that peripheral blood miR-152 and miR-210 levels were independent risk factors for adverse pregnancy outcomes in PE patients (P<0.05). The ROC and DCA results showed that the combination of peripheral blood miR-152 and miR-210 had good predictive value and clinical utility in predicting adverse pregnancy outcomes in PE patients.
Conclusion:
The expression of peripheral blood miR-152 and miR-210 is up-regulated in PE patients, and it is significantly correlated with the severity of the disease and pregnancy outcomes. The combination of the two has a certain reference value and clinical utility for predicting adverse pregnancy outcomes in patients.
2024 Vol. 30 (5): 849-856 [
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38
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857
Analysis of the Correlation between Levels of Lp-PLA2 LP(a) ApoB/ApoA-1 and the Severity and Prognosis of Acute Cerebral Infarction
XUE Han, GAO Jing, WANG Dongjing, et al
DOI: 10.3969/j.issn.1006-6233.2024.05.028
Objective:
To explore the correlation between the expression levels of lipoprotein-associated phospholipase A2 (Lp-PLA2), lipoprotein a [LP(a)], apolipoprotein B (ApoB)/apolipoprotein A-1 (ApoA-1) and the severity and prognosis of acute cerebral infarction (ACI).
Methods:
A total of 122 ACI patients admitted to our hospital from January 2022 to January 2023 were selected as the case group. According to the severity of the disease, they were divided into mild (54 cases), moderate (49 cases), and severe (19 cases) groups. Another 97 healthy individuals who underwent physical examinations during the same period were selected as the control group. According to the prognosis, the patients were divided into a good prognosis group (88 cases) and a poor prognosis group (34 cases). Differences in basic information and biochemical indicators between the case group and control group were compared, and the impact of the expression levels of Lp-PLA2, LP(a), and ApoB/ApoA-1 on the severity of the disease and prognosis was analyzed.
Results:
The age, smoking history, number of hypertension cases, systolic blood pressure, LDL-C, LP(a), and Lp-PLA2 levels in the case group were higher than those in the control group (P<0.05), while the HDL-C level was lower in the case group than in the control group (P<0.05). Multivariate logistic regression analysis indicated that age, smoking history, hypertension, systolic blood pressure, HDL-C, LDL-C, LP(a), Lp-PLA2, and ApoB/ApoA-1 were independent risk factors for ACI (P<0.05). The levels of Lp-PLA2, LP(a), and ApoB/ApoA-1 in the severe group were significantly higher than those in the mild and moderate groups (P<0.05), and the levels in the moderate group were significantly higher than those in the mild group (P<0.05). Spearman correlation analysis showed that the levels of Lp-PLA2, LP(a), and ApoB/ApoA-1 were positively correlated with the severity of the disease (r=0.796, 0.718, 0.451; P<0.05). The levels of Lp-PLA2, LP(a), and ApoB/ApoA-1 in the good prognosis group were significantly higher than those in the poor prognosis group (P<0.05). ROC curve analysis showed that the combined prediction AUC was 0.987.
Conclusion:
Elderly individuals, those with abnormal blood pressure and lipid levels, and those with poor lifestyle habits are more prone to ACI. The levels of Lp-PLA2, ApoB/ApoA-1 are significantly correlated with the severity and prognosis of ACI. Monitoring these levels can effectively assess the progression of the patient's condition and has high clinical value.
2024 Vol. 30 (5): 857-862 [
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33
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863
Effects of Compound Matrine Injection Combined with Xihuang Pill on Cancer Pain and Fatigue Median Survival and Complication Rate in Patients with Advanced Colorectal Cancer Undergoing Palliative Care
FAN Yongqiang, XU Yufeng, CHEN Lin
DOI: 10.3969/j.issn.1006-6233.2024.05.029
Objective:
To investigate the effects of compound Kushin injection combined with Xihuang Pill on cancer pain and fatigue, median survival and complication rate in patients with advanced colorectal cancer receiving palliative care.
Methods:
A total of 153 patients with advanced colorectal cancer treated in The First Affiliated Hospital of Chengdu Medical College from January 2020 to May 2021 were randomly divided into control group (conventional palliative care), monotherapy group (conventional palliative care + Xihuang pill) and combination group (conventional palliative care + Xihuang pill + compound matrine injection), with 51 cases in each group. Cancer pain [numerical pain scale (NRS)], cancer fatigue [Piper Revised Fatigue Scale (PFS-R)], survival [Functional status score (KPS)], inflammatory factors [interleukin-6 (IL-6), C-reactive protein (CRP), and IL-8, IL-1β], immune function (total T lymphocytes, total B lymphocytes, NK cells) were compared between the 3 groups before treatment, 1 cycle and 3 cycles after treatment, and the tumor control effect, complications, toxicity and median survival of the 3 groups were compared.
Results:
The proportion of stable tumor in combination group was higher than that in control group and monotherapy group, and the proportion of progressive patients was lower than that in monotherapy group and control group, and the proportion of patients in monotherapy group was lower than that in control group (P<0.05). After 1 and 3 cycles of treatment, the NRS score and PFS-R score in combination group were lower than those in monotherapy group and control group, and the monotherapy group was lower than that in control group, and the KPS score was higher than that in monotherapy group and control group, and the monotherapy group was higher than that in control group (P<0.05). After 1 and 3 cycles of treatment, the total T lymphocytes, total B lymphocytes and NK cells in combination group were higher than those in monotherapy group and control group, and monotherapy group was higher than control group (P<0.05). The levels of IL-6, CRP, IL-8 and IL-1β in combination group were lower than those in monotherapy group and control group after 1 and 3 cycles of treatment, and those in monotherapy group were lower than those in control group (P<0.05). The incidence of constipation, insomnia, intestinal obstruction, intestinal bleeding, hypothermia and infection in combination group was lower than that in monotherapy group and control group, and the incidence of constipation, insomnia, intestinal obstruction, hypothermia and infection in drug group was lower than that in control group (P<0.05). The incidence of nausea, vomiting and myelosuppression in combination group was lower than that in monotherapy group and control group, and the incidence in monotherapy group was lower than that in control group (P<0.05). After 2 years of follow-up, 2 cases were lost to follow-up, 7 cases died in the combination group, 20 cases died in the monotherapy group, and 34 cases died in the control group. The median survival time of combination group, single drug group and control group was 18, 14 and 13 months, respectively, and the median survival time of combination group was longer than that of monotherapy group and control group (P<0.05).
Conclusion:
Compound matrine injection combined with Xihuang Pill effectively alleviated cancer pain and fatigue, improve the quality of life, enhance immune function, reduce inflammatory response and complication rate in patients with advanced colorectal cancer in palliative care.
2024 Vol. 30 (5): 863-870 [
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28
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871
Effect and Prognosis of EGFR-TKI Combined with Chemotherapy in the Treatment of EGFR-Mutated Advanced Lung Adenocarcinoma
LIN Chengkui, ZHU Chengsheng, WANG Yu
DOI: 10.3969/j.issn.1006-6233.2024.05.030
Objective:
To investigate the effect and prognosis of EGFR-TKI combined with PP (pemetrexed+cisplatin) chemotherapy in the treatment of epidermal growth factor receptor (EGFR)-mutated advanced lung adenocarcinoma.
Methods:
This study was conducted from January 2019 to January 2021. A total of 105 patients with EGFR-mutated advanced lung adenocarcinoma were selected, and divided into the combination group (54 cases) and the control group (51 cases) according to the treatment plan. The control group was treated with second-generation EGFR-TKI (dacomitinib). The combination group was treated with dacomitinib and PP chemotherapy, with 21 days as a cycle. Efficacy, tumor markers and progression-free survival were compared between the two groups.
Results:
The objective remission rate and disease control rate in the combination group (27.78% and 88.89%) were higher than those in the control group (9.8% and 68.63%) (P<0.05). The difference values of serum carbohydrate antigen 125 (CA125), carcinoembryonic antigen (CEA), tissue polypeptide antigen (TPA) and cytoplasmic thymidine kinase (TK1) levels before and after treatment in the combination group were higher than those in the control group (P<0.05). The incidence rates of thrombocytopenia, diarrhea, enteritis, stomatitis, decreased appetite, liver dysfunction, renal dysfunction and rash in the two groups were close (P>0.05). The 1-year and 2-year progression-free survival rates in the combination group (81.48% and 35.19%) were higher than those in the control group (62.75% and 17.65%) (P<0.05). The median progression-free survival time of the combination group and control group were 19.0 months and 15.1 months. There is a difference in the survival curves between the two groups (P<0.05).
Conclusion:
Second-generation EGFR-TKI combined with PP chemotherapy can significantly prolongs progression-free survival, without increasing adverse reactions.
2024 Vol. 30 (5): 871-874 [
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