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2024 Vol. 30, No. 2
Published: 2024-02-29
 
177 Effects of Remimazolam on Brain Tissue Injury and TLR4/MyD88/NF-κB Pathway in Rats with Traumatic Brain Injury
WANG Dongya, QIAO Dan, CHEN Weijia, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.01
Objective: To investigate the effects of Remimazolam on brain tissue injury and the Toll like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear factor-κB (NF-κB) pathway in rats with traumatic brain injury (BI). Methods: BI rat model was constructed; All rats were grouped into Control group, brain injury group (BI group), low, medium and high dose Remimazolam groups (Rem-L, Rem-M, Rem-H groups), and high dose Remimazolam+TLR4 activator LPS group of (Rem-H+LPS group); the brain tissue water content was detected; ELISA was applied to detect the levels of inflammatory factors such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in rat brain tissue; HE staining was applied to observe the morphological changes of brain tissue; TUNEL method was applied to detect neuronal apoptosis in brain tissue; Western blot method was applied to detect the expression of TLR4, MyD88, NF-κB, p-NF-κB p65, Bax, and Bcl-2 proteins in brain tissue. Results: Compared with Control group, brain neurons in BI group were degenerated and necrotic, the number of neurons decreased, the volume decreased, and the injury was serious. Neural function score, brain tissue water content, neuronal apoptosis rate, levels of TNF-α, IL-1β, IL-6 and expressions of Bax, TLR4, MyD88, NF-κB, p-NF-κB p65 were increased. Bcl-2 expression decreased (P<0.05); Compared with BI group, the neuronal cell damage in brain tissue of rats in Rem-L, Rem-M and Rem-H groups was gradually reduced, and the cell structure was relatively clear. The neural function score, brain tissue water content, neuronal apoptosis rate, TNF-α, IL-1β, IL-6 levels and the expressions of Bax, TLR4, MyD88, NF-κB, p-NF-κB p65 were decreased successively, while the expression of Bcl-2 was increased successively (P<0.05); Compared with the Rem-H group, the brain tissue injury of the rats in the Rem-H+LPS group was aggravated, the neural function score, brain tissue water content, neuronal apoptosis rate, the levels of TNF-α, IL-1β, IL-6 and the expressions of Bax, TLR4, MyD88, NF-κB, p-NF-κB p65 were increased, and the expression of Bcl-2 was decreased (P<0.05). Conclusion: Remimazolam can improve brain tissue injury in rats with traumatic brain injury by inhibiting the TLR4/MyD88/NF-κB pathway.
2024 Vol. 30 (2): 177-182 [Abstract] ( 104 ) HTML (1 KB)  PDF (1969 KB)  ( 55 )
182 Effect of Inhibition of Autophagy on Protection of β-Cell Damage by Cyanidin-3-O-Glucoside in High Glucose and High Fat Environment
LI Heng, ZHANG Cuilan
DOI: 10.3969/j.issn.1006-6233.2024.02.02
Objective: To investigate the effects of cyanidin-3-O-glucoside (C3G) on islet beta cell injury and its mechanism in high glucose and high fat (HGHF) environment. Methods: The pancreatic β cells were treated with C3G (10, 20, 30, 40, 50 μmol/L) for 24 h, and the cell viability was detected by CCK-8 assay. The HGHF environment was established by using the culture medium containing 25 mmol/L glucose and 0.5 mmol/L palmitate, and C3G (10, 20, 30, 40, 50 μmol/L) was added to treat the pancreatic β cells for 24 h. The cell viability was detected by CCK-8 assay. The experiment was divided into four groups: control group, HGHF group, HGHF+C3G group, and HGHF+C3G+3-MA group. After the corresponding treatment, CCK-8 assay was used to detect the cell viability of each group. DCFH-DA fluorescent probe was used to detect the ROS level of each group. The levels of malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione (GSH) in each group were detected by colorimetric assay. The insulin secretion of each group was determined by ELISA assay. Real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the mRNA expression levels of autophagy-related genes 5 (Atg5), microtubule-associated protein light chain 3 (LC3), and Beclin-1 in each group. Western blot was used to detect the protein expression levels of LC3-Ⅱ/LC3-Ⅰ, Beclin-1, Bcl-2-associated X protein (Bax), B-cell lymphoma 2 (Bcl-2), and activated caspase-3 (Cleaved Caspase-3) in each group. Results: Compared with the control group, the cell viability of the C3G group (10, 20, 30, 40, 50 μmol/L) was increased (P<0.05), and the cell viability of the HGHF group was decreased (P<0.05). Compared with the HGHF group, the addition of 20, 30, 40, 50 μmol/L C3G in HGHF environment could significantly increase the cell viability (P<0.05). Compared with the HGHF group, the cell viability of the HGHF+C3G group was increased (P<0.05), the ROS content and MDA content were decreased (P<0.05), the SOD activity and GSH-Px content were increased (P<0.05), the insulin secretion level was increased (P<0.05), the mRNA relative expression levels of Atg5, LC3, and Beclin-1 were up-regulated (P<0.05), the LC3-Ⅱ/LC3-Ⅰ ratio and Beclin-1 protein relative expression levels were up-regulated (P<0.05), the Bax and Cleaved Caspase-3 protein relative expression levels were down-regulated, and Bcl-2 protein relative expression level was up-regulated (P<0.05). However, when C3G was added to the HGHF environment and 3-methyladenine (3-MA), an autophagy inhibitor, was also used, the protective effect of C3G on pancreatic β cells in HGHF environment disappeared. Conclusion: C3G has a protective effect on pancreatic β cells in HGHF environment. It can improve cell viability, inhibit oxidative stress damage, and this effect may be related to the promotion of autophagy.
2024 Vol. 30 (2): 182-188 [Abstract] ( 52 ) HTML (1 KB)  PDF (1435 KB)  ( 39 )
189 Exploring the Molecular Mechanism of Propofol-Mediated Inhibition of Colorectal Cancer through TM2D1-Mediated Ferroptosis
LU Yan, PIAO Zongfang, TAN Xinmin, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.03
Objective: To investigate the molecular mechanism by which propofol inhibits colorectal cancer through TM2D1-mediated ferroptosis. Methods: The expression of TM2D1 in normal and colorectal cancer tissues was assessed. Human colorectal cancer SW480 cells were exposed to 50 μM propofol to examine cell viability. SW480 cells were transfected with an overexpressed TM2D1 vector and treated with propofol. The effects of propofol on cell viability, colony formation, cell proliferation, iron levels, ROS production, and ferroptosis were evaluated. Results: TM2D1 is highly expressed in colorectal cancer tissues. Propofol inhibited the viability of SW480 cells, leading to a significant down-regulation of TM2D1. Propofol also suppressed the proliferation and colony formation of colorectal cancer cells, while increasing cellular iron and ROS levels. Furthermore, propofol altered the expression of proteins associated with ferroptosis, including the up-regulation of CHAC1 and PTGS2, and the inhibition of GPX4 expression in CRC cells. Overexpression of TM2D1 blocked the effects of propofol on CRC cells, and the differences were statistically significant (P<0.05). Conclusion: Propofol may induce ferroptosis in colorectal cancer cells by down-regulating TM2D1 expression.
2024 Vol. 30 (2): 189-194 [Abstract] ( 54 ) HTML (1 KB)  PDF (1853 KB)  ( 65 )
194 Ulinastatin Alleviates Acute Kidney Injury in Severe Acute Pancreatitis Models by Inhibiting Ferroptosis
ZHOU Shaoying, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.04
Objective: To investigate whether Ulinastatin can reduce acute kidney injury in severe acute pancreatitis models by inhibiting ferroptosis. Methods: A total of 24 mature male Wistar rats were randomly divided into 4 groups with 6 rats in each group: The histological changes of renal tubule in Control group (Control), severe acute pancreatitis group (SAP), severe acute pancreatitis group + low-dose Ulinastatin group (SAP+UTI-L), severe acute pancreatitis group + high-dose Ulinastatin group (SAP+UTI-H) were evaluated by H&E staining, and renal function was evaluated according to BUN and SCr content. The activity levels of TNF-α, IL-6, SOD, H2O2, MDA, ROS and GPX4 were detected by ELISA kit. Iron content detection kit was used to determine iron levels in kidney tissues. Western blot and qRT-PCR were used to detect the protein expression levels and mRNA levels of ACSL4, GPx4 and FTH1, respectively. Results: Compared with Control group, the renal tissue injury score in SAP group was significantly increased, SCr and BUN levels were significantly increased (P<0.05), TNF-α and IL-6 levels, MDA, H2O2 and ROS levels were significantly increased (P<0.01), and renal iron concentration was significantly increased (P<0.01). The activity of GPX4 was significantly decreased (P<0.01), the protein expression levels and mRNA levels of iron death related proteins GPX4 and FTH1 were significantly decreased (P<0.01), and the protein expression levels and mRNA levels of ACSL4 were significantly increased (P<0.01). After Ulinastatin treatment, the renal tissue injury score was significantly decreased. The levels of Cr (P<0.05) and BUN (P<0.01) were significantly decreased, the levels of inflammation and oxidative stress were decreased, the concentration of renal iron was significantly decreased, the activity of GPX4, the expression levels of GPX4 and FTH1 protein and mRNA levels were significantly increased, and the protein expression levels and mRNA levels of ACSL4 were significantly decreased. Conclusion: Ulinastatin can reduce acute kidney injury in rats with severe acute pancreatitis by inhibiting ferroptosis.
2024 Vol. 30 (2): 194-199 [Abstract] ( 78 ) HTML (1 KB)  PDF (1739 KB)  ( 45 )
199 MiR-92a-3p Targeting KLF4 to Promote Malignant Progression of Hepatocellular Carcinoma
CAI Qingshan, ZHENG Jianxing, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.05
Objective: To investigate the effect of microRNA-92a-3p (miR-92a-3p) on hepatocellular carcinoma (HCC) and its molecular mechanism. Methods: qRT-PCR was used to detect the expression of miR-92a-3p and Krüppel-like factor 4 (KLF4) in human HCC cell lines SMMC-7721, Bel-7402, HepG2, Hep3B, and human normal liver cell line HL-7702. SMMC-7721 and Bel-7402 cells were divided into eight groups: Inhibitor NC group, miR-92a-3p Inhibitor group, mimic NC group, miR-92a-3p mimic group, mimic NC+oe-NC group, mimic NC+oe-KLF4 group, miR-92a-3p mimic +oe-NC group, and miR-92a-3p mimic +oe-KLF4 group. qRT-PCR was used to detect the mRNA expression levels of miR-92a-3p and KLF4 in cells. Western blot was used to detect the protein expression levels of KLF4. MTT proliferation assay was used to detect cell viability. Scratch healing and Transwell invasion assays were used to detect cell migration and invasion abilities, respectively. TargetScan was used to analyze the targeting relationship between miR-92a-3p and KLF4, and the results were verified by dual luciferase assay. Results: Compared with normal cells, miR-92a-3p was highly expressed in HCC cells, and KLF4 was lowly expressed in HCC cells (P<0.05). Compared with the normal expression group, down-regulation of miR-92a-3p significantly inhibited the proliferation, migration, and invasion abilities of SMMC-7721 and Bel-7402 cells (P<0.05). The dual luciferase assay verified the existence of a targeting binding site between miR-92a-3p and KLF4. Overexpression of KLF4 was able to inhibit the proliferation, migration, and invasion of HCC cells, and inhibit the promoting effect of overexpressed miR-92a-3p on HCC cell growth, with statistical significance (P<0.05). Conclusion: miR-92a-3p can promote proliferation, migration, and invasion of HCC cells by targeting down-regulation of KLF4.
2024 Vol. 30 (2): 199-204 [Abstract] ( 73 ) HTML (1 KB)  PDF (1732 KB)  ( 31 )
204 Overexpression of Caspase1 Induces G0/G1 Cell Cycle Arrest and NLRP3 Inflammasome-Mediated Pyroptosis in Human Acute Myeloid Leukemia Cells THP-1
AIKEBAIER Abudureyimu, XU Li, AZIGULI Maimaiti, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.06
Objective: To investigate the effects of caspase1 (CASP1) on the cell cycle and pyroptosis of human acute myeloid leukemia (AML) cells THP-1. Methods: THP-1 cells were cultured and divided into the control group (normal THP-1 cells), pcDNA3.1-null group (negative control for overexpression of CASP1, transfected with 5.0 μg/mL pcDNA3.1-null plasmid into THP-1 cells for 24 h), and pcDNA3.1-CASP1 group (overexpression of CASP1, transfected with 5.0 μg/ml pcDNA3.1-CASP1 plasmid into THP-1 cells for 24 h). Cell proliferation activity was detected by CCK-8 assay. Apoptosis and cell cycle were detected by flow cytometry. CASP1, interleukin (IL)-1β, and IL-18 mRNA expression levels were detected by qRT-PCR. Cell proliferation-related proteins Ki67, proliferating cell nuclear antigen (PCNA), cyclin D1, NOD-like receptor (NLR)-like receptor pyrin domain-containing protein 3 (NLRP3), apoptosis-associated speck-like protein (ASC), CASP1, cleaved-caspase 1 (cleaved-CASP1), IL-1β, IL-18, and cleaved-Gasdermin D were detected by Western blot. Results: Compared with the control group, there were no significant differences in cell proliferation activity, apoptosis, and cell cycle changes in the pcDNA3.1-null group (P>0.05). Compared with the control group, cell proliferation activity was reduced, G0/G1 cell cycle was blocked, and the relative expression levels of Ki67, PCNA, and cyclin D1 were decreased (P<0.05) in the pcDNA3.1-CASP1 group. The relative expression levels of NLRP3, ASC, CASP1, cleaved-CASP1, IL-1β, IL-18, and cleaved-Gasdermin D were increased (P<0.05). Compared with the pcDNA3.1-null group, there were no significant differences in apoptosis in the pcDNA3.1-CASP1 group (P>0.05). However, cell proliferation activity was reduced, G0/G1 cell cycle was blocked, and the relative expression levels of Ki67, PCNA, and cyclin D1 were decreased (P<0.05). The relative expression levels of NLRP3, ASC, CASP1, cleaved-CASP1, IL-1β, IL-18, and cleaved-Gasdermin D were increased (P<0.05). Conclusion: Overexpression of CASP1 induces G0/G1 cell cycle arrest and NLRP3 inflammasome-mediated pyroptosis in AML cells THP-1.
2024 Vol. 30 (2): 204-210 [Abstract] ( 35 ) HTML (1 KB)  PDF (1727 KB)  ( 39 )
210 Influencing Factors of Adjacent Vertebral Fractures after Percutaneous Vertebral Augmentation: A Meta-analysis
ZHANG Zhilong, CUI Yushi, WU Hongfei, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.07
Objective: To analyze the related factors of adjacent vertebral fractures after percutaneous vertebral augmentation (PVA) in patients with osteoporosis vertebral compression fracture (OVCF) by a systematic review. Methods: Databases including PubMed, Web of Science, Embase, Cochrane Library, CNKI, Wanfang Data Knowledge Service platform, Vip.com, and Chinese biomedical literature database, were searched to screen relevant studies on influencing factors of adjacent vertebral fractures after PVA in patients with OVCF. The search period extended from the establishment of each database to May 21, 2023. Meta-analysis was performed using RevMan 5.4 and Stata 17. Results: Finally, 43 case-control studies were included, all of which were high quality literatures, with a total sample size of 10601 cases. Meta-analysis results showed that risk factors for adjacent vertebral fractures after PVA included advanced age[OR=1.13, 95%CI (1.08,1.17)], low body mass index [OR=2.29, 95% CI (1.36, 3.84)], smoking [OR=2.77, 95% CI (1.52, 5.05)], low bone mineral density [OR= 3.88, 95% CI (2.64, 5.70)], diabetes [OR=2.25, 95%CI (1.57, 3.21)], scoliosis [OR=4.41, 95% CI (2.52, 7.73)], more injured vertebra [OR=2.02,95%CI(1.43, 2.85)], bone cement leakage [OR=3.47, 95% CI (2.77, 4.35)], excessive injection of bone cement [OR=2.94, 95% CI (2.50, 3.46)], high recovery rate of vertebral body height [OR=1.28, 95% CI (1.10, 1.49)], excessive correction of local Cobb Angle [OR=1.27, 95% CI (1.04, 1.55)]; The protective factor was anti-osteoporosis therapy [OR=0.32, 95% CI (0.21, 0.49)]. Conclusion: Advanced age, low body mass index, smoking, low bone mineral density, chronic obstructive pulmonary disease, diabetes, scoliosis, more injured vertebra, bone cement leakage, excessive injection of bone cement, high recovery rate of vertebral height, and excessive correction of local Cobb Angle are risk factors for adjacent vertebral fractures after PVA. In clinical practice, the controllable risk factors should be actively controlled, and a reasonable anti-osteoporosis treatment program should be formulated to reduce the adverse prognosis. The conclusions drawn above still require further validation through more large-scale and high-quality studies.
2024 Vol. 30 (2): 210-218 [Abstract] ( 39 ) HTML (1 KB)  PDF (1470 KB)  ( 25 )
218 Correlation Study of Endoplasmic Reticulum Phosphorylation Modification and Immune Response in Pulmonary Tuberculosis Infection
CHEN Cheng, DENG Yong, LU Xueming, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.08
Objective: To observe the correlation between phosphorylation of endoplasmic reticulum (ER) and immune response after pulmonary tuberculosis infection. Methods: In this observational study, we recruited adult tuberculosis patients (n=19) who were treated in our hospital from January 2021 to June 2023 and family contacts (healthy contacts) who had no symptoms of tuberculosis (n=20). Blood samples were collected from all patients before treatment, and the characteristics of specific T cell response, cytokine production and expression of phosphorylated protein kinase-like endoplasmic reticulum kinase (PERK) and activator of transcription 3(STAT3) of Mycobacterium tuberculosis were analyzed. Results: Compared with healthy contacts, the levels of IL-17 and IL-22 in whole blood samples stimulated by PPD in tuberculosis patients decreased significantly (P<0.05), and the levels of IL-6 and IL-10 increased significantly (P<0.05). Compared with healthy contacts, the spontaneous concentrations of IL-10, IL-6 and IFN-γ in tuberculosis patients were higher (P<0.05). In the absence of IL-6 stimulation in vitro, the level of PERK in CD4+ T cells of tuberculosis patients was significantly higher than that of healthy contacts (P<0.001). Adding IL-6 led to a significant increase in the level of PERK in CD4+ T cells of healthy contacts compared with those without IL-6 stimulation in vitro (P<0.01). Compared with healthy contacts, CD4+ T cells of tuberculosis patients had higher expression of STAT3 protein (P<0.05). In all donors and tuberculosis patients, there was a significant positive correlation between the expression of PERK and STAT3 in CD4+ T cells (rho=0.571, 0.503, all P<0.05). The ratio of CD40L/IL-2 co-expressing T cells and CD40L/IFN-γ co-expressing T cells was negatively correlated with STAT3 expression only in tuberculosis patients (rho = -0.481, -0.705, P=0.032, <0.001). Conclusion: This study provides evidence that the PERK/STAT3 signaling pathway related to ER phosphorylation modification may drive the immunosuppressive/antiproliferative T cell response in tuberculosis patients.
2024 Vol. 30 (2): 218-223 [Abstract] ( 30 ) HTML (1 KB)  PDF (1935 KB)  ( 48 )
223 Efficacy of Fire Needle Combined with Blood-letting Acupoint Pricking in the Treatment of Herpes Zoster and Its Impact on Peripheral Blood Th17 Treg Cell Ratio and Related Cytokines
XIE Lanqing, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.09
Objective: To explore the efficacy of fire needle combined with blood-letting acupoint pricking in the treatment of herpes zoster (HZ) and analyze its impact on peripheral blood Th17, regulatory T (Treg) cell ratio, and related cytokines. Methods: Eighty patients with HZ admitted to our hospital from January 2019 to December 2020 were selected and divided into control group and study group according to the random single or double number allocation principle, with 40 cases in each group. The control group received western medicine antiviral treatment, while the study group received fire needle combined with blood-letting acupoint pricking plus western medicine antiviral treatment. The treatment effects, recovery process, pre and post-treatment HZ symptom scores (erythema, vesicles, skin lesion pain), peripheral blood Th17, Treg cell ratios were compared. The levels of Th17/Treg cell-related cytokines (IL-6, IL-17, TGF-β1) and neural pain factors [prostaglandin E2 (PGE2), serotonin (5-HT)] were detected using CytoFLEX flow cytometry and ELISA, respectively. The pain levels (VAS scores) were compared before and after treatment. Results: The total effective rate in the study group 97.50% was higher than that in the control group 80.00% (P<0.05). The study group showed better recovery process than the control group (P<0.05). Three days after treatment, erythema score, vesicle score, and skin lesion pain score in the study group were (1.24±0.35), (1.94±0.42), and (1.27±0.36), respectively, lower than those in the control group (P<0.05). Seven days after treatment, erythema score, vesicle score, and skin lesion pain score in the study group were (0.82±0.24), (0.94±0.30), and (0.68±0.22), respectively, lower than those in the control group (P<0.05). Three and seven days after treatment, the Th17 cell ratios in peripheral blood were (1.58±0.46)% and (1.05±0.33)% in the study group, Treg cell ratios were (4.21±0.95)% and (3.53±0.87)%, serum IL-6 levels were (14.53±2.61) pg/mL and (10.12±2.14) pg/mL, IL-17 levels were (22.68±3.86) pg/mL and (18.65±3.42) pg/mL, PGE2 levels were (126.85±12.69) ng/L and (95.42±9.35) ng/L, 5-HT levels were (65.32±14.19) ng/mL and (42.86±10.74) ng/mL, all lower than those in the control group (P<0.05). Three and seven days after treatment, TGF-β1 levels were (0.48±0.05) pg/mL and (0.51±0.05) pg/mL in the study group, higher than those in the control group (P<0.05). Three and seven days after treatment, VAS scores in the study group were (3.15±0.82) and (1.87±0.59), respectively, lower than those in the control group (P<0.05). Conclusion: The treatment of herpes zoster with fire needle combined with blood-letting acupoint pricking is effective, improves clinical symptoms, shortens the recovery process, and relieves pain. The mechanism of action may be related to correcting the imbalance of Th17/Treg cells.
2024 Vol. 30 (2): 223-230 [Abstract] ( 38 ) HTML (1 KB)  PDF (1688 KB)  ( 29 )
230 Prognostic Value of Blood NT-proBNP CRP and NLR in Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease and Pulmonary Infection
WU Qinqin, WANG Yanyan, LI Jia, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.010
Objective: To investigate the prognostic value of blood N-terminal prohormone of brain natriuretic peptide (NT-proBNP), C-reactive protein (CRP) and neutrophil to lymphocyte ratio (NLR) in patients with AECOPD and pulmonary infection. Methods: From December 2019 to December 2021, 120 patients with AECOPD and pulmonary infection were admitted to the hospital. Their clinical data were analyzed retrospectively. Patients enrolled were divided into survival group (n=86) and death group (n=34) according to the prognosis. General data, blood NT-proBNP, CRP and NLR were compared. The prognostic value of the three in patients with AECOPD and pulmonary infection was analyzed using the receiver operating characteristic (ROC) curve. Results: Compared with the death group, blood NT-proBNP, CRP and NLR in the survival group were lower (P<0.05). ROC curve analysis results showed that the area under the curve (AUC) of NLR for predicting the prognosis of patients with AECOPD and pulmonary infection was the largest, with sensitivity of 73.53% and specificity of 63.95%. The AUC of CRP was the smallest, with sensitivity of 47.06% and specificity of 82.56% (P>0.05). The AUC of parallel prediction with the combination of blood NT-proBNP, CRP and NLR was 0.700. The sensitivity and specificity were 97.06% and 43.02%. Conclusion: Blood NT-proBNP, CRP and NLR are of high value in the prognosis of patients with AECOPD and pulmonary infection. In clinical practice, NT-proBNP, CRP and NLR can be used as auxiliary indicators to predict the patient's condition, thus improving the prognosis.
2024 Vol. 30 (2): 230-234 [Abstract] ( 68 ) HTML (1 KB)  PDF (1280 KB)  ( 38 )
234 Association of Serum CAR LMR and T Lymphocyte Subsets with Prognosis of Multiple Myeloma Patients
DA Haili, ZHANG Xuguang, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.011
Objective: To investigate the association of serum C-reactive protein to albumin ratio (CAR), lymphocyte to monocyte ratio (LMR), and T lymphocyte subsets with the prognosis of multiple myeloma (MM) patients. Methods: A total of 105 patients with multiple myeloma diagnosed and treated from January 2020 to January 2022 were selected as the study objects and set as the observation group. A total of 75 healthy controls were enrolled as the control group. MM patients were divided into poor prognosis group (n=34) and good prognosis group (n=71) according to whether recurrence or death occurred within 2 years of follow-up after treatment. Serum CAR, LMR, and T lymphocyte subsets (CD3+, CD4+, CD8+, CD4+/CD8+) were compared. Logistic regression analysis was used to analyze the effects of CAR, LMR, CD3+, CD4+, CD8+ and CD4+/CD8+ on the prognosis of MM patients. ROC curve analysis was used to analyze the AUC values, sensitivity, and specificity of CAR, LMR, CD3+, CD4+, CD8+, CD4+/CD8+ and T lymphocyte subsets to predict the prognosis of MM patients. Results: The CAR and CD8+ levels were significantly higher, while the LMR, CD3+, CD4+, and CD4+/CD8+ levels were significantly lower in the observation group than in the control group (P<0.05). The CAR and CD8+ levels were significantly higher, while the LMR, CD3+, CD4+, and CD4+/CD8+ levels were significantly lower in the poor prognosis group than in the good prognosis group (P<0.05). Binary Logistic regression model analysis showed that the increase of CAR and CD8+ and the decrease of LMR, CD3+, CD4+, CD4+/CD8+ would affect the prognosis of MM patients (P<0.05). ROC curve analysis showed that the combined parameters of CAR, LMR, CD3+, CD4+, CD8+, CD4+/CD8+ and T lymphocyte subsets predicted the prognosis of MM patients with AUC values of 0.637, 0.792, 0.726, 0.767, 0.669, 0.750, 0.853, respectively (P<0.05). Conclusion: Serum CAR, LMR, and T lymphocyte subsets showed abnormal expression trends in MM patients. The detection of their changes is helpful for providing important reference for the assessment of the prognosis of MM patients.
2024 Vol. 30 (2): 234-238 [Abstract] ( 46 ) HTML (1 KB)  PDF (1307 KB)  ( 33 )
239 Serum Levels of Carbohydrate Antigen 125 (CA125) Growth Differentiation Factor-15 (GDF-15) and Soluble Triggering Receptor Expressed on Myeloid Cells-1 (sTREM-1) in Patients with Chronic Heart Failure and their Correlation with Cardiac Function: An Analysis
ZHANG Guoyong, MA Zheng, ZHANG Lin, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.012
Objective: To explore the expression of serum carbohydrate antigen 125(CA125), growth differentiation factor -15(GDF-15) and soluble myeloid cell trigger receptor -1(sTREM-1) in patients with chronic heart failure and their correlation with cardiac function. Methods: The clinical data of 96 patients with CHF admitted to our hospital from June 2022 to June 2023 were analyzed retrospectively, including 46 patients with mild and moderate heart failure (NYHA grade Ⅰ-Ⅲ) and 50 patients with severe heart failure (NYHA grade Ⅳ). In addition, 48 healthy people who underwent physical examination in our hospital during the same period were selected as the healthy control group according to the ratio of 2:1. The serum levels of CA125, GDF15 and sTREM-1 were compared with the cardiac function indexes, and the correlation between the serum levels of CA125, GDF15 and sTREM-1 and the cardiac function indexes was analyzed. The ROC curve was used to analyze the efficacy of serum levels of CA125, GDF15 and sTREM-1 in diagnosing chronic heart failure. Results: The levels of CA125, GDF15, and sTREM-1 in the study group were higher than those in the control group (P<0.05). FS and EF in the study group were lower than those in the control group, while LAD and LVEDD were higher than those in the control group (P<0.05). Person correlation analysis showed that CA125, GDF15, and sTREM-1 were negatively correlated with FS and EF (r=-0.605/-0.617, -0.516/-0.512, -0.572/-0.613, P<0.05), and positively correlated with LAD and LVEDD (r=0.827/0.818, 0.819/0.834, 0.846/0.878, P<0.05). The levels of CA125, GDF15, and sTREM-1 in the severe heart failure group were higher than those in the mild to moderate heart failure group (P<0.05). Binary Logistic Regression Analysis showed that serum CA125, GDF15, and sTREM-1 were risk factors for severe heart failure (P<0.05). The AUC, sensitivity, and specificity of serum CA125, GDF15, and sTREM-1 in evaluating the severity of chronic heart failure were 0.761, 0.695, and 0.822, respectively. The AUC for assessing the severity of chronic heart failure by prediction scoring ROC curves was 0.848 with a sensitivity of 84.0% and a specificity of 82.6%. Conclusion: Serum levels of CA125, GDF15, and sTREM-1 are upregulated in patients with CHF and are closely related to cardiac function. They can be used as effective indicators to evaluate the severity of heart failure, with better combined evaluation performance.
2024 Vol. 30 (2): 239-244 [Abstract] ( 62 ) HTML (1 KB)  PDF (1376 KB)  ( 46 )
244 Distribution of Tuberculosis Drug Resistance Genes in Patients with Hepatitis B Complicated with Mycobacterium Tuberculosis Infection
MENG Ying, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.013
Objective: To examine the M. tuberculosis strain diversity and describe the frequency and molecular mechanisms of resistance against first-line TB drugs in TB/HBV co-infected patients. Methods: A total of 100 strains of Mycobacterium tuberculosis from TB/HBV co-infected patients were analyzed retrospectively, among which Beijing strain was the most common (49%), followed by EAI strain (35%), and a few strains included Haarlem(2.0%), LAM(1.0%), MANU(3.0%) and T(4.0%). All strains were isolated from sputum and pleural effusion samples of patients from 2014 to 2018. Drug susceptibility testing, spoligotyping and 24-locus Mycobacterial Interspersed Repetitive Unit (MIRU-24 typing) were performed, and the rpoB, katG, inhA and inhA promoter, rpsL, rrs and embB genes were sequenced in all drug resistant isolates identified. Results: In total, 42/100 (42.0%) strains showed drug resistance; 9 (9.0%) were multi-drug resistant (MDR). Resistance rates were 25.0% (25/100) to isoniazid, 10.0% (10/100) to rifampicin, 5.0% (5/100) to ethambutol, and 40.0% (40/100) to streptomycin. Among rifampicin-resistant strains, 90% (9/10) of the isolates with rifampicin resistance had mutations in the RRDR (codons 507-533). Among isoniazid-resistant strains, mutations were found in 100% and 21% of katG and inhA (including the inhA promoter), respectively; their multidrug resistance was mainly associated with the Ser315Thr mutation in the katG gene (7/9, 77.8%). 59.5% of the strains with resistance belonged to the Beijing spectrum, and 66.7% of the MDR strains belonged to the Beijing spectrum. Conclusion: Nearly half (42%) of M. tuberculosis isolates from TB/HBV co-infected patients are drug-resistant strains, among which Beijing lineage is the main strain.
2024 Vol. 30 (2): 244-250 [Abstract] ( 43 ) HTML (1 KB)  PDF (1228 KB)  ( 95 )
250 Effect Analysis of Antagonistic Strategy of Shugeng Sodium Gluconate and Neostigmine on Lung Function in Patients Undergoing Lobectomy
GUO Yudong, MA Xiaoyan, LI Wenjuan, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.014
Objective: To explore the effects of rocuronium-sugammadex and rocuronium-neostigmine reversal strategies on pulmonary function in patients undergoing lobectomy. Methods: Eighty-six patients who underwent lobectomy from January 2021 to December 2022 were selected as the research subjects. They were randomly divided into an observation group (receiving rocuronium-sugammadex reversal strategy) and a control group (receiving rocuronium-neostigmine reversal strategy), with 43 cases in each group. The forced expiratory volume in one second (FEV1), forced vital capacity (FVC), peak expiratory flow rate (PEFR), norepinephrine (NE), propofol (Cor), blood oxygen pressure (PaO2), remifentanil dose, oxygenation index (PaO2/FiO2), postoperative autonomous breathing recovery time, postoperative consciousness recovery time, muscle relaxant onset time, extubation time, post-anesthesia care unit (PACU) stay time, and postoperative complications were compared between the two groups. Results: Repeated measures analysis of variance showed that there were significant time effects, group effects, and time-group interaction effects in FEV1, FVC, PEFR, NE, Cor, PaO2, and PaO2/FiO2 in both groups (P<0.05). At the time points of tracheal extubation and removal of chest drainage tube, the FEV1, FVC, and PEFR in the observation group were higher than those in the control group (P<0.05). At T1, T2, and T3 time points, NE and Cor in the observation group were lower than those in the control group (P<0.05). At the time of skin incision, 15 minutes before extubation, and immediately after leaving the PACU, PaO2 and PaO2/FiO2 in the observation group were higher than those in the control group (P<0.05). The propofol and remifentanil doses in the observation group were lower than those in the control group, and the postoperative autonomous breathing recovery time, PACU stay time, postoperative consciousness recovery time, muscle relaxant onset time, and extubation time were shorter than those in the control group (P<0.05). The incidence of nausea and vomiting, chills, hypotension, and pulmonary complications on postoperative days 1 and 7 in the observation group was lower than that in the control group (P<0.05). Conclusion: The use of rocuronium-sugammadex for muscle relaxation reversal in patients undergoing lobectomy is beneficial for reducing the impact on pulmonary function and lowering the incidence of postoperative complications.
2024 Vol. 30 (2): 250-254 [Abstract] ( 34 ) HTML (1 KB)  PDF (1217 KB)  ( 31 )
255 Expression and Clinical Significance of Stanniocalcin 2 (STC2) in Cervical Cancer Tissues
DENG Rundao, FU Xiafei
DOI: 10.3969/j.issn.1006-6233.2024.02.015
Objective: To investigate the expression of calcitonin STC2 in cervical cancer tissue and evaluate its clinical significance. Methods: A retrospective study was conducted on cervical cancer patients (n=88) from Dongguan People's Hospital from January 2022to May 2022. Immunohistochemical method was used to determine the expression of STC2 in cervical tissue of patients. Real time fluorescence quantitative PCR was used to detect the gray value of STC2. compare the grayscale values of STC2 mRNA between cervical cancer tissue and normal tissue, analyze the expression of STC2 in different clinical characteristics of cervical cancer, and perform logistic regression analysis on their correlation. Results: The grayscale values of STC2 mRNA in cervical cancer tissues were higher than those in adjacent tissues, with statistical significance (P<0.05); The positive expression of STC2 in cervical cancer tissue showed statistically significant differences in different infiltration depths, differentiation levels, lymph node metastasis, and TNM staging, P<0.05; Multivariate logistic regression analysis showed that depth of infiltration, degree of differentiation, lymph node metastasis, and TNM staging were independent influencing factors for STC2 positivity; The 5-year Overall survival(OS) and Progression-Free Survival(PFS) of the STC2 positive group were lower than those of the STC2 negative group, with a statistically significant difference (P<0.05); Pearson analysis showed that the expression of STC2 was negatively correlated with OS and PFS (r=-0.747, -0.683, P<0.05). Conclusion: STC2 has a high expression in cervical cancer tissues, which has nothing to do with age, tumor diameter, pathological classification, depth of invasion, degree of differentiation and TNM stage, but is related to lymph node metastasis, which should be paid greatattention to.
2024 Vol. 30 (2): 255-259 [Abstract] ( 48 ) HTML (1 KB)  PDF (1201 KB)  ( 28 )
259 Application Value of NT-pro BNP in the Diagnosis of Frailty and Prognostic Assessment in Elderly Heart Failure Patients
YE Fei, CHEN Dandan, YAO Yao
DOI: 10.3969/j.issn.1006-6233.2024.02.016
Objective: To explore the application value of N-terminal pro-B-type natriuretic peptide (NT-pro BNP) in the diagnosis of elderly heart failure with frailty and assessment of prognosis. Methods: A total of 115 cases of elderly heart failure from January 2020 to March 2022 were selected. According to the Fried frailty diagnostic scale, patients were divided into frailty group, pre-frailty group, and non-frailty group. The diagnostic value of NT-pro BNP level for frailty and its correlation with frailty severity were analyzed. The frailty group was further divided into a better prognosis group and a poor prognosis group based on major adverse events occurring within 12 months of enrollment, and the prognostic value of NT-pro BNP level was analyzed. Results: The levels of NT-pro BNP, BNP, cTnⅠ, CK, and CK-MB in the frailty group, pre-frailty group, and non-frailty group showed a decreasing trend. The indicators in the frailty group were higher than those in the pre-frailty group, and the pre-frailty group was higher than the non-frailty group (P<0.05). The AUC value of NT-pro BNP level for diagnosing frailty in elderly heart failure patients was 0.773, with a 95% CI of 0.6850.889, P<0.001, sensitivity of 87.10%, and specificity of 70.00%. Conclusion: NT-pro BNP levels are abnormally elevated in elderly heart failure patients with frailty. This marker has diagnostic value for frailty and prognostic assessment in patients, providing valuable insights into their diagnosis and prognosis.
2024 Vol. 30 (2): 259-263 [Abstract] ( 35 ) HTML (1 KB)  PDF (1299 KB)  ( 29 )
264 Regulatory Effect of Irisin on Bone Metabolism in Postmenopausal Osteoporosis Rats by Regulating the Hippo/YAP Signaling Pathway
HOU Xiuxi, LIU Bo, XING Baorui, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.017
Objective: To investigate the effects of irisin on bone metabolism and Hippo/Yes associated protein (YAP) signaling pathway in postmenopausal osteoporosis (PMOP) rats. Methods: A PMOP rat model was established using bilateral ovariectomy. All experimental rats divided into the following groups: control group, PMOP group, irisin low, medium, and high dose groups (Irisin-L, Irisin-M, Irisin-H groups), and high-dose irisin+YAP inhibitor Vitipofen group (Irisin-H+VTPF group); bone mineral density meter was applied to measure bone mineral density (BMD); Micro-CT was applied to analyze the average thickness of trabeculae (Tb.Th), percentage of trabecular area (Tb.Ar), number of trabeculae (Tb.N), and trabecular separation (Tb.SP); HE staining was applied to observe the morphological changes of femoral tissue; ELISA method was applied to detect the levels of bone metabolism biomarkers specific alkaline phosphatase (BALP), procollagen type Ⅰ N-terminal propeptide (PINP), osteocalcin (OC), and core binding factor-α1 (CBF-α1) in serum; Western blot method was applied to detect the expression levels of transcriptional co-activator with PDZ-binding motif (TAZ), YAP, and p-YAP proteins. Results: Compared with the Control group, the trabecular bone structure of the femoral bone tissue in the PMOP group was disordered, the trabecular bone thickness was decreased, the bone marrow space was significantly increased, and even mild fractures occurred. The levels of BMD, Tb.Th, Tb.Ar, Tb.N, BALP, PINP, OC, RANKL, TAZ, YAP, and p-YAP were significantly decreased, and Tb.Sp was significantly increased (P < 0.05). Compared with the PMOP group, the trabecular bone structure of the femoral bone tissue in the Irisin-L, Irisin-M, and Irisin-H groups was gradually arranged in order, the bone marrow space was reduced, and the femoral bone tissue damage was gradually alleviated. The levels of BMD, Tb.Th, Tb.Ar, Tb.N, BALP, PINP, OC, RANKL, TAZ, YAP, and p-YAP were significantly increased in order, and Tb.Sp was significantly decreased in order (P < 0.05). Compared with the Irisin-H group, the trabecular bone structure of the femoral bone tissue in the Irisin-H + VTPF group was disordered, the bone marrow space was significantly increased, the femoral bone tissue damage was aggravated, and the levels of BMD, Tb.Th, Tb.Ar, Tb.N, BALP, PINP, OC, RANKL, TAZ, YAP, and p-YAP were significantly decreased, and Tb.Sp was significantly increased (P < 0.05). Conclusion: Irisin can prevent and treat osteoporosis by regulating bone metabolism in OVX osteoporotic rats through the activation of the Hippo/YAP signaling pathway.
2024 Vol. 30 (2): 264-269 [Abstract] ( 58 ) HTML (1 KB)  PDF (1935 KB)  ( 41 )
269 Value of CTA in Evaluating Cerebrovascular Stenosis in Patients with Ischemic Stroke
WU Jing, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.018
Objective: To observe the value of CT angiography (CTA) in evaluating the degree of cerebrovascular stenosis in patients with ischemic stroke (IS). Methods: The clinical data of 110 patients with IS admitted to the hospital from January 2020 to June 2023 were analyzed retrospectively. All patients were examined with conventional transcranial Doppler ultrasound and CTA. Digital subtraction angiography (DSA) was taken as the golden standard to compare the value of Doppler ultrasound and CTA in evaluating the degree of cerebrovascular stenosis in patients with IS. Results: DSA found 24 cases of mild cerebrovascular stenosis, 63 cases of moderate stenosis, 13 cases of severe stenosis, and 10 cases of occlusion. Among them, the proportion of extracranial arteries (56.36%) is higher than that of intracranial arteries (43.64%). The sensitivity, specificity and accuracy of Doppler ultrasound in evaluating moderate or more severe cerebrovascular stenosis in patients with IS were 54.17%, 77.91% and 72.73%. The sensitivity, specificity and accuracy of CTA were 95.83%, 88.37% and 90.00%. The sensitivity, accuracy, positive predictive value and negative predictive value of CTA in evaluating moderate or more severe cerebrovascular stenosis in patients with IS were significantly higher than those of Doppler ultrasound (P<0.05). There was no significant difference in specificity between the two methods (P>0.05). Conclusion: CTA is of high value in evaluating the degree of cerebrovascular stenosis in patients with IS, and its sensitivity and accuracy are higher than those of conventional Doppler ultrasound. It can clearly show the patients' cerebrovascular lesions, providing reference for clinical diagnosis and treatment.
2024 Vol. 30 (2): 269-273 [Abstract] ( 54 ) HTML (1 KB)  PDF (1347 KB)  ( 49 )
273 Evaluation of Combination of Osteopontin and Cystatin C in Diagnosis of Coronary Heart Disease
Sun Qitian, Xue Tingyun, Yan Zhenrong, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.019
Objective: To evaluate the combination of osteopontin and cystatin C in diagnosis of coronary heart disease. Methods: Serum levels of OPN and Cys-C were measured in 148 patients who underwent coronary angiography in our hospital. These patients were grouped according to the diseases they had and clinical data of them were collected. Results: Logistic regression analysis showed that OPN and Cys-c could independently predict CHD.ROC curve showed that the AUC of osteopontin combined with cystatin C in the diagnosis of CHD was 0.830, the sensitivity was 76.42%, and the specificity was 81.16%. The results of comparison of OPN and Cys-c levels between different groups showed OPN level was significantly higher in the UAP group than that in SAP group, but significantly lower than that in the AMI group. The level of Cys-c increased in UAP group compared with that in SAP group and the level of Cys-c decreased in AMI group compared with that in UAP group(P<0.05). But the changes of Cys-c in different groups were not statistically significant(P>0.05). Conclusion: Our study demonstrated OPN and Cys-c have diagnostic values for CHD, and dynamic detections of OPN and Cys-c are helpful for disease monitoring.
2024 Vol. 30 (2): 273-276 [Abstract] ( 43 ) HTML (1 KB)  PDF (1288 KB)  ( 87 )
277 Predictive Value of Fragmented QRS Complex on Electrocardiogram Combined with Serum pro-BNP for Heart Failure in Patients with Acute Anterior Myocardial Infarction
HE Peijuan, WU Jinpan, HU Juanjuan, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.020
Objective: To investigate the predictive value of fragmented QRS (fQRS) complex on electrocardiogram combined with serum pro-brain natriuretic peptide (pro-BNP) for heart failure in patients with acute anterior myocardial infarction (AAMI). Methods: A total of 145 patients with AAMI were admitted to the hospital from February 2020 to February 2023. They were divided into two groups according to the presence or absence of heart failure in 3 months after percutaneous coronary intervention (PCI). General data of patients in the heart failure group and the non-heart failure group were collected. All patients completed routine 12-lead electrocardiogram and serum pro-BNP level detection. Logistic regression analysis was performed to identify the influencing factors of heart failure in patients with AAMI. The value of fQRS complex, pro-BNP, and their combination in predicting heart failure in patients with AAMI was analyzed using the ROC curve. Results: A total of 76 (52.41%) patients developed heart failure and 69 (47.59%) patients did not. Serum creatinine (Scr) level and therapeutic time window after onset in the heart failure group were higher/longer than those in the non-heart failure group. The diseased branches before operation were more than the non-heart failure group (P<0.05). Patients with fQRS complex on electrocardiogram in the heart failure group were more than the non-heart failure group, and pro-BNP level was higher than that in the non-heart failure group (P<0.05). fQRS complex on electrocardiogram and pro-BNP were influencing factors of heart failure in patients with AAMI (P<0.05). For single prediction of heart failure in patients with AAMI, the AUC of pro-BNP was the highest (0.800), with sensitivity of 50.00% and specificity of 85.65%. The sensitivity and specificity of joint prediction with the two indicators were of 84.21% and 69.57%. Conclusion: fQRS and pro-BNP are influencing factors for heart failure in AAMI patients. fQRS and pro-BNP have high prognostic value in HF in AAMI patients. The combination of them can significantly improve sensitivity and has higher prognostic value, which can be used as an auxiliary detection index.
2024 Vol. 30 (2): 277-281 [Abstract] ( 38 ) HTML (1 KB)  PDF (1256 KB)  ( 27 )
281 Clinical Observation of Continuous Veno-Venous Hemofiltration Combined with Hemoperfusion in The Treatment of Severe Acute Organophosphorus Poisoning
QI Xiaoli, XIONG Mingfen, TAO Ning, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.021
Objective: To observe the clinical efficacy of continuous veno-venous hemofiltration (CVVH) combined with hemoperfusion (HP) in the treatment of severe acute organophosphorus poisoning (ASOPP). Methods: The data of 126 patients with ASOPP were retrospectively analyzed. According to the treatment methods, the patients were divided into CVVH+HP group (n=60) and HP group (n=66). The efficacy and treatment were observed. Serum cholinesterase (CHE) level was recorded before treatment (T1) and at 4h (T2), 24h (T3) and 72h (T4) after treatment. The differences of serum inflammatory factors [interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-10(IL-10), tumor necrosis factor-α (TNF-α)] and liver-kidney injury related indicators [cystatin C (CysC), prealbumin (PA), high mobility group protein B1 (HMGB1)] were compared between the two groups at T1 and T3, and the occurrence of complications was observed. Results: The cure rate was 93.33% in CVVH+HP group, which was higher than 78.79% in HP group (P<0.05). There was no statistical significance in the incidence rate of rebound between the two groups (P>0.05). The dosages of atropine and pralidoxime chloride with (143.37±18.72)mg and (10.28±2.62)g in CVVH+HP group were less than (154.18±21.47)mg and (19.57±4.31)g in HP group (all P<0.05). The coma time and hospital stay in CVVH+HP group were (13.38±3.41)h and (7.26±1.58)d, which were shorter than (22.15±4.14)h and (8.42±1.74)d in HP group (all P<0.05). At T2~T4, the level of serum CHE in the two groups was increased gradually, and the increase in CVVH+HP group was greater than that in HP group (all P<0.05). At T1, the levels of serum IL-1β, IL-6, IL-10 and TNF-α and serum CysC, PA and HMGB1 revealed no statistical differences between the two groups (P>0.05). The differences of serum IL-1β, IL-6, IL-10, TNF-α, CysC, PA and HMGB1 at T1-T3 were greater in CVVH+HP group than those in HP group (all P<0.05). There was no statistical difference in the occurrence of complications between the two groups (P>0.05). Conclusion: Compared with HP alone, CVVH+HP for ASOPP can enhance the cure rate, improve the treatment, promote the recovery of serum CHE activity, alleviate the inflammatory response and relieve the organ damage, and it has good safety and certain advantages.
2024 Vol. 30 (2): 281-286 [Abstract] ( 34 ) HTML (1 KB)  PDF (1231 KB)  ( 73 )
287 Serum ASC and NLRP3 Levels and Their Significance in Patients with Rheumatoid Arthritis
JIANG Yongsen, ZHAO Chunnan, ZHANG Zezhi, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.022
Objective: To investigate the predictive value of serum human apoptosis-associated speck-like protein (ASC) and NOD-like receptor heat protein structural domain-related protein 3 (NLRP3) in rheumatoid arthritis (RA) patients. Methods: A total of 88 patients with RA diagnosed at our hospital from November 2021 to June 2023 were enrolled as the RA group, and 88 healthy volunteers were enrolled as the control group. Serum ASC and NLRP3 levels were measured by enzyme-linked immunosorbent assay. Spearman correlation analysis was used to analyze the correlation between serum ASC, NLRP3 levels and DAS28 scores in patients with RA, and logistic regression analysis was used to analyze the influencing factors of RA. Results: The serum ASC levels in the RA group were significantly higher than those in the healthy control group , and the NLRP3 levels were significantly higher than those in the healthy control group , with statistical significance (P<0.05). Logistic regression analysis showed that ASC and NLRP3 were independent risk factors for RA patients (P<0.05). Conclusion: The increased serum ASC and NLRP3 levels are independently correlated with DAS28 scores, suggesting that serum ASC and NLRP3 have potential diagnostic value for RA and its disease activity.
2024 Vol. 30 (2): 287-290 [Abstract] ( 54 ) HTML (1 KB)  PDF (1236 KB)  ( 40 )
290 Relationship between Degenerative Lumbar Spondylolisthesis and Facet Joint Angles and Zygapophyseal Articular Process Root Angles Based on CT Observation
LI Lan, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.023
Objective: To explore the relationship between degenerative lumbar spondylolisthesis (DLS) and facet joint angles and zygapophyseal articular process root angles based on CT observation. Methods: A retrospective analysis was conducted on 169 patients diagnosed with DLS at Sichuan Provincial Orthopedic Hospital from January 2020 to June 2022 (DLS group). Additionally, 169 age-matched patients with lumbar and leg pain but without lumbar spondylolisthesis, who underwent physical examinations during the same period, were selected as the healthy control group. Clinical data were compared between the two groups, and both univariate and multivariate logistic regression analyses were performed to identify factors influencing DLS. Smooth curve fitting was used to analyze the curve relationship between facet joint angles, zygapophyseal articular process root angles, and DLS. A Bayesian network model was constructed, and its predictive performance was validated. Results: Univariate analysis revealed that BMI, intervertebral disc degeneration, systemic joint laxity, changes in lumbar spine structure and curvature, ligament laxity, osteoporosis, decalcification, lumbar facet joint lesions, and comorbid diabetes were higher in the DLS group than in the healthy group (P<0.05). The facet joint angles in the DLS group were smaller than those in the healthy group, and the proportion of individuals with asymmetrical facet joint angles and degeneration degrees of 1 and 2 increased, while the zygapophyseal articular process root angle significantly increased (P<0.05). Multivariate analysis indicated that an increase in BMI, intervertebral disc degeneration, changes in lumbar spine structure and curvature, ligament laxity, osteoporosis, decalcification, systemic joint laxity, lumbar facet joint lesions, comorbid diabetes, decrease in facet joint angles, asymmetry of facet joint angles, degeneration of facet joint angles, and an increase in zygapophyseal articular process root angles were all risk factors for DLS (OR>1, P<0.05). Smooth curve fitting results demonstrated a positive correlation between zygapophyseal articular process root angles and DLS within a certain range, while facet joint angles showed a negative correlation with DLS. The Bayesian network model and predictive reasoning revealed that an increase in BMI, decrease in facet joint angles, asymmetry of facet joint angles, and an increase in zygapophyseal articular process root angles were directly associated with DLS. When the probability of decreased facet joint angles, asymmetry of facet joint angles, and an increase in zygapophyseal articular process root angles reached zero, the occurrence rate of DLS decreased from 50% to 37.2%. Model validation confirmed the good discriminability, accuracy, and effectiveness of the Bayesian network predictive model. Conclusion: CT observation provides a more accurate diagnosis of DLS, and within a certain range, facet joint angles and zygapophyseal articular process root angles are correlated with DLS.
2024 Vol. 30 (2): 290-296 [Abstract] ( 37 ) HTML (1 KB)  PDF (2062 KB)  ( 26 )
296 Comparative Analysis of Efficacy of Masquelet Technique and Ilizarov Technique in the Treatment of Chronic Osteomyelitis
HE Dong, LANG Zhigang, LIN Daqiang, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.024
Objective: To compare the clinical efficacy of Masquelet technique and Ilizarov technique in the treatment of chronic osteomyelitis (CO). Methods: A total of 85 CO patients were randomly divided into two groups by random number table: M group (n=42, Masquelet technique) and I group (n=43, Ilizarov technique). The treatment effects, rehabilitation time, joint function, quality of life, and postoperative complications were compared between the two groups. Results: The excellent and good rate of treatment effect in group I was slightly higher than that in group M (P>0.05). Bone healing time and complete weight-bearing time in group I were longer compared to group M(P<0.05). There were no statistically significant differences in lowa knee score, lowa ankle score and WHOQOL-BREF scores between two groups before surgery (P>0.05), and the above scores in both groups were significantly higher after surgery than those before surgery (P<0.05). There was no significant difference in the changes in the lowa knee score, lowa ankle score, and WHOQOL-BREF score between the two groups before and after surgery (P>0.05). The needle tract infection rate in the I group was higher than that in the M group (P<0.05). Conclusion: Masquelet technology and Ilizarov technology are similar to the efficacy of CO treatment, but Masquelet technology has higher requirements for soft tissues, and patients need to have sufficient self-donor bone mass, which should be selected clinically according to the specific circumstances.
2024 Vol. 30 (2): 296-301 [Abstract] ( 38 ) HTML (1 KB)  PDF (1684 KB)  ( 30 )
301 Application Value of Contrast-Enhanced Ultrasound Parameters on Evaluating Neovascularization Grading and Carotid Plaque Stability in Patients with Acute Cerebral Infarction
CHEN Ye, GONG Yi, WEI Meimei
DOI: 10.3969/j.issn.1006-6233.2024.02.025
Objective: To explore the application value of contrast-enhanced ultrasound (CEUS) parameters on evaluating the neovascularization grading and carotid plaque stability in patients with acute cerebral infarction (ACI). Methods: A total of 115 patients with ACI admitted to our hospital from May 2020 to May 2023 were selected. All patients underwent CEUS examination, and the CEUS parameters [time to peak (Tp), mean transit time (MTT), area under the time-intensity curve (AUCt), peak intensity (PI)] were compared among patients with different neovascularization grades and carotid plaque stability, and the correlation between CEUS parameters and ACI neovascularization grading was analyzed by Spearman correlation analysis. The application value of CEUS parameters on evaluating carotid plaque stability was analyzed by receiver operating characteristic (ROC) curve. Results: CEUS detected 11 cases of neovascularization grade 0, 33 cases of grade 1 and 71 cases of grade 2, accounting for 9.57%, 28.70% and 61.74% respectively. The Tp and MTT in the three groups were manifested as grade 0>grade 1>grade 2 (P<0.05), and the AUCt and PI were shown as grade 0<grade 1<grade 2 (P<0.05). Spearman correlation analysis found that Tp and MTT were negatively correlated with neovascularization grading (P<0.05) while AUCt and PI were positively correlated with neovascularization grading (P<0.05). CEUS detected 85 cases of soft plaque (unstable) (73.91%) and 30 cases of stable plaque, including 14 cases of hard plaque (12.17%) and 16 cases of mixed plaque (13.91%). Tp and MTT in the three groups showed soft plaque < mixed plaque < hard plaque (P<0.05) while AUCt and PI were shown as soft plaque> mixed plaque > hard plaque (P<0.05). ROC curve analysis showed that Tp, MTT, AUCt, PI and combined detection (parallel diagnosis) had predictive efficiency on ACI soft plaques (P<0.05), with AUCs of 0.778, 0.724, 0.830, 0.817 and 0.833 respectively, and single detection of AUC had the highest efficiency, but was still lower than the combined detection. Conclusion: The neovascularization grading in patients with ACI is negatively correlated with Tp and MTT, and is positively correlated with AUCt and PI. The detection of Tp, MTT, AUCt and PI can guide the neovascularization grading of patients. In addition, the Tp and MTT of patients with carotid unstable plaque are lower than those in patients with good stability, and the AUCt and PI are higher than those in patients with good stability. CEUS parameters are effective in assessing the ACI carotid plaque stability.
2024 Vol. 30 (2): 301-306 [Abstract] ( 34 ) HTML (1 KB)  PDF (1943 KB)  ( 58 )
306 Correlation Study and Risk Factor Analysis of Non-Erosive Gastroesophageal Reflux Disease with Mucosal Bulge Lesions at the Esophagogastric Junction
LONG Xiaotian, ZHANG Meng, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.026
Objective: To explore the correlation between non-erosive gastroesophageal reflux disease (NERD) and the mucosal bulge lesions of esophagogastric junction, and to provide the basis for the diagnosis of NERD under endoscopy. Risk factors of NERD were also analyzed. Methods: A prospective study was conducted on patients who underwent gastroscopy from January 2022 to December 2022. Patients meeting the inclusion criteria were categorized into the NERD group, while asymptomatic patients who underwent gastroscopy during the same period were included in the control group. General demographic and endoscopic factors were compared between the two groups, and the relationship between NERD and mucosal bulge lesions at the esophagogastric junction was analyzed. Results: Univariate analysis revealed significant differences in gender, age, BMI, dietary habits, drinking, NSAID use, gastroesophageal flap valve classification, mucosal bulge lesions at the esophagogastric junction, bile reflux, and HP infection between the NERD and control groups (P<0.05). Logistic regression analysis identified female gender, high BMI, poor dietary habits, drinking, NSAID use, gastroesophageal flap valve relaxation (III+IV), mucosal bulge lesions at the esophagogastric junction, bile reflux, and HP infection as factors influencing NERD. The sensitivity and specificity of mucosal bulge lesions at the esophagogastric junction for NERD were 45.83% and 86.27%, respectively. Conclusion: Mucosal bulge lesions at the esophagogastric junction are significantly correlated with NERD, serving as supportive diagnostic evidence and enhancing diagnostic reliability. Female gender, high BMI, drinking, NSAID use, poor dietary habits, gastroesophageal flap valve relaxation (Ⅲ+Ⅳ), and bile reflux are independent risk factors for NERD, while HP infection is a protective factor.
2024 Vol. 30 (2): 306-312 [Abstract] ( 46 ) HTML (1 KB)  PDF (1593 KB)  ( 38 )
312 Clinical Characteristics of Heatstroke and Analysis of Factors Affecting Prognosis
GUO Xiujuan, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.027
Objective: To investigate the clinical characteristics and the related factors affecting the prognosis of heatstroke (HS). Methods: The clinical data of 80 patients with HS admitted in our hospital from July 2015 to September 2020 were retrospectively analyzed. The general demographic data, clinical characteristics, laboratory test indicators, treatment and outcome were collected. The last follow-up time was January 2023. HS was divided into exertional HS group (n=71) and classical HS group (n=9) according to the state of onset and pathogenesis. The two groups of patients were compared in terms of general data and laboratory indicators. The patients were divided into survival group (n=66) and death group (n=14) according to their prognosis, and regression analysis was performed on the prognostic factors. Results: Of the 80 HS patients, 71 were EHS and 9 were CHS, of whom 5 had a history of hypertension. HS patients were more likely to develop the disease in July-August, with 41 cases (51.25%) in July, 26 cases (32.50%) in August, and 13 cases (16.25%) in September. The proportion of patients with a body temperature ≥40℃, shock, and coma was significantly higher in the EHS group than in the CHS group (P<0.05). The proportions of patients with convulsions, incontinence, arrhythmia, and ataxia were not significantly different between the two groups (P>0.05). Compared with the CHS group, the EHS group had lower levels of alanine aminotransferase (ALT), activated partial thromboplastin time (APTT), fibrinogen, and potassium, but higher levels of D-dimer, aspartate aminotransferase (AST), prothrombin time (PT), and calcium (P<0.05). There was no significant difference in white blood cell count, platelet count, creatinine, or myoglobin between the two groups (P>0.05). Compared with the death group, the survival group had lower levels of white blood cell count, platelet count, D-dimer, AST, ALT, creatinine, PT, APTT, and potassium, but higher levels of calcium (P<0.05). There was no significant difference in myoglobin or fibrinogen between the two groups (P>0.05). Logistic multivariate analysis showed that D-dimer and calcium were significantly associated with the prognosis of HS (P<0.05). Conclusion: D-dimer and serum calcium are independent prognostic factors of HS, and can be used as prognostic indicators. Early monitoring of hematological indicators in HS patients is helpful to take corresponding treatment measures.
2024 Vol. 30 (2): 312-316 [Abstract] ( 45 ) HTML (1 KB)  PDF (1207 KB)  ( 42 )
316 Comparison of Surgical Outcomes and Complications of Subxiphoid Thoracoscopic Surgery and Intercostal Thoracoscopic Surgery for Anterior Mediastinal Tumors
LI Chunlei, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.028
Objective: To investigate the surgical outcomes and complications of subxiphoid thoracoscopic surgery and intercostal thoracoscopic surgery for anterior mediastinal tumors. Methods: A total of 100 patients with anterior mediastinal tumors who underwent thoracoscopic surgery at Xuancheng People's Hospital from January 2020 to January 2023 were enrolled in this study. According to the surgical approach, the patients were divided into the subxiphoid group (subxiphoid thoracoscopic surgery, 60 cases) and the intercostal group (intercostal thoracoscopic surgery, 40 cases). The perioperative indicators (intraoperative blood loss, postoperative drainage volume, operation time, number of pain pump uses), rehabilitation indicators (drainage tube retention time, postoperative analgesic drug use time, hospital stay), different time points of pain visual analog scale (VAS) scores, and postoperative 7, 15, 30, and 45 days Karnofsky performance status (KPS) scores, and complication rates (pleural effusion, pneumothorax, atelectasis, pulmonary infection) were compared between the two groups. Results: The intraoperative blood loss in the sternotomy group was (24.67±3.21) mL, which was lower than that in the intercostal group [(40.13±4.29)mL, P<0.05]; the postoperative drainage volume was (151.95±17.12) mL, which was lower than that in the intercostal group [(306.50±30.52)mL, P<0.05]; the operation time was (66.00±6.59) min, which was shorter than that in the intercostal group [(91.65±10.51)min, P<0.05]; the number of pain pump uses was (6.07±1.02) times, which was lower than that in the intercostal group [(7.10±1.22)times, P<0.05]. The drainage tube retention time in the sternotomy group was (17.20±3.19) h, which was lower than that in the intercostal group [(21.77±3.97)h, P<0.05]; the postoperative analgesic drug use time was (1.85±0.92) d, which was shorter than that in the intercostal group [(3.10±0.90)d, P<0.05]; the hospital stay was (6.10±1.32) d, which was shorter than that in the intercostal group [(8.75±1.66)d, P<0.05]. The VAS scores of patients in the sternotomy group were (2.02±0.43) points before surgery, which were not significantly different from those in the intercostal group [(2.00±0.45)points, P>0.05]; the VAS scores were (4.57±1.44) points at 1 day after surgery, which were significantly lower than those in the intercostal group [(5.83±1.48)points, P<0.05]; the VAS scores were (3.00±1.01) points at 2 days after surgery, which were significantly lower than those in the intercostal group [(4.38±1.39)points, P<0.05]; the VAS scores were (2.40±0.62) points at 3 days after surgery, which were significantly lower than those in the intercostal group [(3.42±0.81)points, P<0.05]. The KPS score of subxiphophorus group was (60.13±3.20) points at 7 days after operation, which was significantly higher than that of intercostal group [(57.38±4.20) points, P<0.05]. 15 days after surgery, the score was (65.78±3.09), significantly higher than that in intercostal group [(62.17±3.76), P<0.05]. The score at 30d after operation was (71.50±3.71) points, significantly higher than that in intercostal group [(68.10±3.86) points, P<0.05]. The score at 45 days after operation was (81.67±5.07), significantly higher than that in intercostal group [(75.45±5.20), P<0.05]. The total incidence of complications was lower than that of intercostal group, the difference was statistically significant (P<0.05). Conclusion: Thoracoscopy via subxiphoid approach is ideal for the treatment of anterior mediastinal tumors, which can effectively improve patients' pain and functional status and reduce the occurrence of complications.
2024 Vol. 30 (2): 316-321 [Abstract] ( 53 ) HTML (1 KB)  PDF (1469 KB)  ( 27 )
322 Perinatal Risk Factors for Late Preterm Neonatal Respiratory Distress Syndrome with Acute Respiratory Distress Syndrome
HUANG Fengzhen, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.029
Objective: To investigate the perinatal risk factors for late preterm neonatal respiratory distress syndrome (NRDS) with acute respiratory distress syndrome (ARDS). Methods: A retrospective analysis was conducted on cases of late preterm NRDS admitted to the neonatal intensive care unit of a certain hospital from September 2019 to January 2023. The patients were divided into the observation group (NRDS with ARDS) and the control group (simple NRDS) according to whether or not they developed ARDS. The perinatal risk factors of the two groups were compared. Results: A total of 92 cases were included, with 44 cases in the observation group and 48 cases in the control group. There were no significant differences in sex, gestational age, body weight, and mode of delivery between the two groups (P>0.05). The incidence of late-term respiratory tract infection, late-term vaginitis, late-term vitamin D deficiency, meconium-stained amniotic fluid, and asphyxia was higher in the observation group than in the control group, with statistically significant differences (P<0.05). The incidence rates of gestational diabetes, gestational hypertension, prenatal lung stimulation therapy, premature rupture of membranes, umbilical cord entanglement, placenta previa, placental abruption, low body weight, and intrauterine distress revealed no statistical significance in both groups (P>0.05). The variables with statistical significance in the univariate analysis (late-term respiratory tract infection, late-term vaginitis, late-term vitamin D deficiency, meconium-stained amniotic fluid, and asphyxia) were included in the logistic regression analysis. The results of the stepwise logistic regression analysis showed that late-term respiratory tract infection (OR=9.613, 95%CI 1.056-87.489, P<0.05), late-term vaginitis (OR=11.469, 95%CI 1.286-102.294, P<0.05), and late-term vitamin D deficiency (OR=7.499, 95%CI 1.887-29.802, P<0.05) were all statistically significant, and were associated with late preterm NRDS with ARDS. Conclusion: Late-term respiratory tract infection, late-term vaginitis, and late-term vitamin D deficiency are independent risk factors for late preterm NRDS with ARDS.
2024 Vol. 30 (2): 322-325 [Abstract] ( 38 ) HTML (1 KB)  PDF (1189 KB)  ( 46 )
326 Clinical Observation of Xingpi Yanger Combined with Meclofenoxate Hydrochloride Capsule in Treating Infantile Enuresis
WAN Juan, LIU Jianying, TANG Guangqun
DOI: 10.3969/j.issn.1006-6233.2024.02.030
Objective: To explore the clinical efficacy of Xingpi Yanger combined with Meclofenoxate Hydrochloride Capsules in the treatment of enuresis in children. Methods: 148 children with enuresis in our hospital from May 2020 to July 2022 were randomly divided into control group (n=74) and investigation group (n=74) according to random number method. The control group was treated with oral meclofenoxate hydrochloride capsules, and the investigation group was treated with oral meclofenoxate hydrochloride capsules + Xingpi Yanger granules for 4 weeks. To evaluate the clinical efficacy of the two groups and compare the TCM symptom score, enuresis times, sleep awakening disorder score, awakening threshold, bladder capacity, urine osmotic pressure and antidiuretic hormone (ADH) levels before and after treatment in the two groups. The recurrence situation was recorded within 3 months-treatment. Results: The overall response rate of investigation group was 85.14%, higher than control group with 64.86%; After treatment, the TCM symptom score, enuresis frequency, sleep awakening disorder score and awakening threshold index of the two groups were decreased, and the investigation group was lower than the control group; Bladder capacity, urine osmolality, and concentration levels of ADH increased in both groups after treatment, and were higher in the investigation group than in the control group, all these differences were statistically significant (P<0.05).After a follow-up of 3 months, the recurrence rate in study group (8.11%) was statistically lower than that in control group (20.27%). Conclusion: Xingpi Yanger combined with meclofenoxate hydrochloride capsules can significantly improve the clinical efficacy of enuresis in children and reduce the recurrence rate, which is worthy of clinical promotion.
2024 Vol. 30 (2): 326-330 [Abstract] ( 34 ) HTML (1 KB)  PDF (1203 KB)  ( 28 )
330 Effect of Rivaroxaban Combined with Alteplase on Coagulation Function and Expression of GRP78 and GRP94 in Patients with Acute Pulmonary Embolism
SUN Jinglan, ZHOU Hongyan, WANG Ying, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.031
Objective: To observe the effects of rivaroxaban combined with alteplase on coagulation function and the expression of glucose regulatory protein 78 (GRP78) and glucose regulatory protein 94 (GRP94) in patients with acute pulmonary embolism. Methods: A total of 104 patients with acute pulmonary embolism admitted to our hospital from January 2021 to December 2022 were selected as subjects and divided into two groups by simple random method. All patients were given absolute bed rest, analgesia, anti-infection, oxygen inhalation and other conventional treatment, conventional group (n=52) plus alteplase treatment, combined group (n=52) plus rivaroxaban plus alteplase treatment. The incidence of bleeding events in the two groups was analyzed. The differences of coagulation function, thrombus elasmogram, GRP78, GRP94, pulmonary artery pressure and blood gas indexes between the two groups were detected. Results: The activated partial thromboplastin time (APTT), prothrombin time (PT) and α Angle and reaction time in the thromboxelasticity chart of the two groups were higher after treatment than before treatment, and the difference of R and α Angle in the APTT, PT and thromboxelasticity chart before and after treatment in the combined group was greater than that in the conventional group (P<0.05). Fibrinogen (Fib) and D-dimer (D-D) in the two groups were lower after treatment than before treatment, and the difference of Fib and D-D in the combined group was greater than that in the conventional group (P<0.05). There was no significant difference in coagulation time (K) between the two groups before and after treatment (P>0.05). GRP78 and GRP94 in the two groups were lower after treatment than before treatment, and the difference of GRP78 and GRP94 before and after treatment in the combined group was greater than that in the conventional group (P<0.05). The partial pressure of arterial oxygen (PaO2) in the two groups was higher after treatment than before treatment, and the difference of PaO2 in the combined group was greater than that in the conventional group (P<0.05). The partial blood pressure of carbon dioxide (PaCO2) of the two groups was lower after treatment than before treatment, and the difference of PaCO2 before and after treatment in the combined group was greater than that in the conventional group (P<0.05). There was no significant difference in the incidence of bleeding events between the two groups (P>0.05). Conclusion: Rivaroxaban combined with alteplase in the treatment of acute pulmonary embolism can improve the coagulation function, reduce the expression of GRP78 and GRP94, and increase the blood gas index.
2024 Vol. 30 (2): 330-335 [Abstract] ( 42 ) HTML (1 KB)  PDF (1237 KB)  ( 114 )
335 Effects of Sintilimab Combined with Chemotherapy on Short-Term Efficacy and Serum CEA, sTim-3 sLAG-3 and T Lymphocyte Subsets in Patients with Advanced Gastric Cancer
LIU Zhao, REN Yuchuan, ZHANG Yapeng, et al
DOI: 10.3969/j.issn.1006-6233.2024.02.032
Objective: To investigate the effects of Sintilimab combined with chemotherapy on short-term efficacy and serum carcinoembryonic antigen (CEA), immune function, and T lymphocyte subsets in patients with advanced gastric cancer. Methods: A total of 84 patients with advanced gastric cancer treated from May 2020 to May 2022 were randomly divided into an observation group and a control group (42 cases each). The control group received conventional chemotherapy, while the observation group received additional Sintilimab. Both groups underwent three cycles of continuous treatment (3 months). Clinical outcomes after 1 month of chemotherapy, occurrence of toxicities, and changes in serum tumor markers (CA, CEA, MG-Ag), immune function (sTim-3, sLAG-3), and T lymphocyte subsets (CD3+, CD4+, CD8+) were compared between the two groups before and after 3 cycles of treatment. The 1-year survival rate was recorded during follow-up. Results: After 1 month of chemotherapy, the observation group showed a higher clinical effective rate compared to the control group, with no significant differences in toxicities (P>0.05). After 3 cycles of treatment, both groups exhibited significant reductions in CA, CEA, MG-Ag, sTim-3, and sLAG-3 levels, and the observation group had lower levels than the control group (all P<0.05), CD3+ and CD4+ levels decreased, with the observation group higher than the control group, while CD8+ levels increased, and the observation group had lower levels than the control group (all P<0.05). The 1-year survival rate in the observation group was significantly higher than in the control group (P<0.05). Conclusion: Sintilimab combined with chemotherapy demonstrates significant short-term efficacy, high safety, and the ability to improve immune function in patients with advanced gastric cancer, thereby alleviating the tumor burden.
2024 Vol. 30 (2): 335-340 [Abstract] ( 33 ) HTML (1 KB)  PDF (1267 KB)  ( 28 )
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