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2024 Vol. 30, No. 1
Published: 2024-01-31
1
Effects of Midazolam on Proliferation Apoptosis and Invasion of Osteosarcoma Cells by Regulating the YAP/TAZ Signaling Pathway
AO Tingting, TU Mengjia, ZHANG Xi
DOI: 10.3969/j.issn.1006-6233.2024.01.01
Objective:
To investigate the effects of midazolam (MDZ) on the proliferation, apoptosis and invasion of osteosarcoma (OS) cells by regulating the Yes associated protein (YAP)/PDZ transcriptional activator of transcription (TAZ) signaling pathway.
Methods:
qRT-PCR and western blot were applied to detect the expression of YAP, TAZ mRNA, and proteins in normal osteoblast and OS cell lines, and to screen the optimal intervention cell line. OS cells were intervened with different concentrations of MDZ (0, 12.5, 25, 50, 100, 200 μmoL/L), MTT method was applied to detect cell proliferation activity and screen the optimal intervention concentration; OS cells were randomly divided into control group, MDZ group, pcDNA3.1 group, and pcDNA3.1-YAP/TAZ group, and transfection efficiency was detected using qRT-PCR method; EdU staining, flow cytometry, and Transwell cells were applied to detect cell proliferation, apoptosis, and invasion, respectively; Western blot method was applied to detect the expression of proliferating cell nuclear antigen (PCNA), Bcl-2 associated X protein (Bax), N-cadherin, E-cadherin, and YAP, TAZ proteins; OS nude mouse model was constructed, and grouped into a control group and an MDZ group, immunohistochemical methods were applied to detect the expression of Ki-67, YAP, and TAZ proteins in transplanted tumor tissue, and TUNEL staining was applied to detect apoptosis.
Results:
The expression levels of YAP and TAZ mRNA and protein in OS cell lines were obviously increased (P<0.05); MDZ obviously reduced the expression of PCNA, N-cadherin, YAP, and TAZ, inhibitd the proliferation and invasion of MG63 and U20S cells, increased the expression of Bax and E-cadherin, and promoted cell apoptosis. Overexpression of YAP/TAZ was able to reverse the inhibitory effects of MDZ on the proliferation and invasion of MG63 and U20S cells, and promote the apoptosis of MG63 and U20S cells (P<0.05); in vivo experiments showed that MDZ obviously reduced the mass and volume of transplanted tumors, and the expression levels of Ki-67, YAP, and TAZ, promoted apoptosis of transplanted tumor cells (P<0.05).
Conclusion:
MDZ may inhibit the YAP/TAZ signaling pathway, inhibit the proliferation and invasion of OS cells, and promote apoptosis.
2024 Vol. 30 (1): 1-8 [
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74
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8
The Impact of Si-SETDB1 on Migration and Invasion of Lung Adenocarcinoma Cells through SPG20 Methylation
ZHAO Baoshan, et al
DOI: 10.3969/j.issn.1006-6233.2024.01.02
Objective:
To investigate the methylation levels of SETDB1 and SPG20 in lung adenocarcinoma and explore the impact of downregulating SETDB1 on SPG20 methylation, A549 cell migration, and invasion.
Methods:
Sixty cases of lung adenocarcinoma tissues and normal lung tissues were selected. qRT-PCR was used to detect mRNA relative expression levels, immunohistochemistry and western blot were used to detect protein expression levels, and gene methylation levels were assessed by bisulfite sequencing. Small interfering RNA (siRNA) targeting SETDB1 was constructed and transfected into cells using liposomes. qRT-PCR, Western blot, bisulfite sequencing, MTT assay, scratch healing assay, and Transwell chamber assay were performed to evaluate mRNA and protein expression, gene methylation, cell proliferation, migration, and invasion, respectively.
Results:
qRT-PCR results showed that SPG20 expression was downregulated (P < 0.05), while SETDB1 expression was upregulated (P < 0.05) in lung adenocarcinoma tissues. Western blot results indicated that SETDB1 protein expression in cancer tissues was significantly higher than in normal lung tissues, whereas SPG20 protein exhibited lower expression in cancer tissues, lower than in normal lung tissues. In cancer tissues, the SPG20 gene methylation rate was significantly higher than in normal lung tissues, with a statistically significant difference. SPG20 methylation level was positively correlated with SETDB1 expression. SETDB1 was highly expressed, while SPG20 was lowly expressed in lung adenocarcinoma cells. Conversely, in normal bronchial epithelial cells, SETDB1 was lowly expressed, and SPG20 was highly expressed. RNA interference with SETDB1 significantly reduced SETDB1 mRNA and protein expression in A549 cells, decreased SPG20 gene methylation, and inhibited cell proliferation, migration, and invasion.
Conclusion:
There is a correlation between methyltransferase SETDB1 and SPG20 methylation in lung adenocarcinoma. SETDB1 may serve as a catalytic enzyme for SPG20 methylation.
2024 Vol. 30 (1): 8-15 [
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41
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15
Effects of CircRTN4 on the Malignant Biological Behavior of Glioblastoma Cells by Regulating the miR-224-5p/MYT1L Axis
LIU Yinggang, LI Weimin, LONG Yong, et al
DOI: 10.3969/j.issn.1006-6233.2024.01.03
Objective:
To investigate the effect of circRNA RTN4 (CircRNA) on the malignant biological behavior of glioblastoma (GM) cells by regulating miR-224-5p/myelin transcription factor-1-like (MYT1L) axis.
Methods:
U251 cells in logarithmic growth phase were divided into the following groups: blank group, negative control (sh NC) group, silenced CircRTN4 shRNA (sh CircRTN4) group, sh CircRTN4 + inhibitor control (inhibitor NC) group, and sh CircRTN4 + miR-224-5p inhibitor (miR-224-5p inhibitor) group. Transwell experiment, flow cytometry, CCK-8, qRT-PCR, Western blot, and dual-luciferase assay were used to detect cell migration and invasion, apoptosis, proliferation, CircRTN4, miR-224-5p, MYT1L mRNA expression, proliferation protein (ki-67), apoptosis protein (cleaved Caspase-3), and MYT1L protein expression. The targeting relationships between miR-224-5p and CircRTN4, MYT1L were verified. qRT-PCR was used to detect the mRNA expression levels of CircRTN4, miR-224-5p, and MYT1L in GBM cell lines (U118, U87, U251, LN229) and normal human astrocytes (NHA) cells.
Results:
In U118, U87, U251, and LN229 cells, CircRTN4 and MYT1L mRNA expression increased significantly, while miR-224-5p expression decreased significantly compared to NHA cells (P<0.05). miR-224-5p had targeting relationships with CircRTN4 and MYT1L. Compared with the blank group and sh NC group, the sh CircRTN4 group showed significantly decreased migration, invasion, 24h, 48h A450 values, ki-67, CircRTN4, MYT1L mRNA, and protein expression, increased apoptosis rate, and cleaved Caspase-3 expression (P<0.05). Compared with the sh CircRTN4 + inhibitor NC group, the sh CircRTN4 + miR-224-5p inhibitor group showed significantly increased migration, invasion, 24h, 48h A450 values, ki-67, MYT1L mRNA, and protein expression, decreased apoptosis rate, and cleaved Caspase-3 expression (P<0.05). CircRTN4 expression showed no statistical difference (P>0.05). In vivo experiments demonstrated that interfering with CircRTN4 significantly inhibited tumor mass in nude mice (P<0.05).
Conclusion:
Silencing CircRTN4 regulates the miR-224-5p/MYT1L axis and inhibits the malignant biological behavior of GM cells.
2024 Vol. 30 (1): 15-22 [
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41
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23
Regulatory Effect of Honeysuckle Polyphenol Extracts on miR-100-5p in Non-Small Cell Lung Cancer NCI-H1299 Cell Invasion and Migration
SHEN Xingyong, BAI Shuang, et al
DOI: 10.3969/j.issn.1006-6233.2024.01.04
Objective:
To investigate the impact of honeysuckle polyphenol crude extract on the malignant biological behavior of non-small cell lung cancer (NSCLC) NCI-H1299 cells and the molecular mechanisms involved.
Methods:
NCI-H1299 cells were divided into NC group, paclitaxel group, and low, medium, and high-dose groups of honeysuckle polyphenol crude extract. Additionally, miR-100-5p group, miR-NC group, anti-miR-100-5p + high-dose group, and anti-miR-NC + high-dose group were established. MTT assay was used to detect cell viability, Transwell assay for cell migration and invasion, Western blot for E-Cadherin, N-Cadherin, Vimentin, and Wnt/β-catenin pathway-related protein expression, and real-time fluorescence quantitative PCR (RT-qPCR) for miR-100-5p expression.
Results:
After treatment with different concentrations of honeysuckle polyphenol crude extract, NCI-H1299 cell viability decreased, and the number of cell migration and invasion decreased. E-Cadherin and miR-100-5p expression levels increased, while N-Cadherin and Vimentin expression levels decreased (P<0.05). Overexpression of miR-100-5p increased NCI-H1299 cell viability, cell migration [(226±20.03) cells], and invasion [(171±15.03) cells], while E-Cadherin expression level decreased (0.34±0.03), and N-Cadherin (0.88±0.08) and Vimentin expression levels increased (P<0.05). Downregulating miR-100-5p could reverse the effects of honeysuckle polyphenol crude extract on NCI-H1299 cell proliferation, migration, invasion, and the Wnt/β-catenin pathway.
Conclusion:
Honeysuckle polyphenol crude extract may inhibit the proliferation, migration, and invasion of NSCLC NCI-H1299 cells by upregulating miR-100-5p expression, thereby suppressing the activation of the Wnt/β-catenin signaling pathway.
2024 Vol. 30 (1): 23-28 [
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43
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29
The Regulatory Effect of LncRNA PURPL on the miR-342-3p/PIK3R1 Axis and its Impact on the Malignant Biological Behavior of Hepatocellular Carcinoma Cells
WANG Hongmei, CHEN Sirui, DU Hong
DOI: 10.3969/j.issn.1006-6233.2024.01.05
Objective:
To investigate the impact of long non-coding RNA (PURPL) on the miR-342-3p/phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) axis and its effects on the malignant biological behavior of hepatocellular carcinoma (HCC) cells.
Methods:
Cell Experiments: Si-NC, si-PURPL, si-PURPL+inhibitor NC, si-PURPL+miR-342-3p inhibitor were separately transfected into HCC cells (Huh-7) and named si-NC group, si-PURPL group, si-PURPL+inhibitor NC group, si-PURPL+miR-342-3p inhibitor group, while untreated Huh-7 cells were designated as the NC group. Dual-luciferase reporter gene experiments verified the relationship between LncRNA PURPL, miR-342-3p, and PIK3R1. qRT-PCR was used to detect the expression of LncRNA PURPL and miR-342-3p in human normal liver cells (L02) and Huh-7 cells. Western blot assessed PIK3R1 protein levels in L02 and Huh-7 cells. MTT assay evaluated Huh-7 cell proliferation, flow cytometry measured apoptosis rates, Transwell assay determined invasive cell numbers, and scratch tests assessed cell migration. In Vivo Experiments: A xenograft mouse model was established with the same cell treatment groups. Tumor volume and weight were measured.
Results:
Cell experiment results showed that compared with the si-NC group, si-PURPL group had significantly decreased OD490 values, scratch healing rates, invasive cell numbers, LncRNA PURPL, and PIK3R1 levels in Huh-7 cells (P<0.05). Apoptosis rates and relative expression of miR-342-3p significantly increased (P<0.05). Inhibiting miR-342-3p expression weakened the inhibitory effects of silencing LncRNA PURPL on Huh-7 cell proliferation, migration, invasion, and promotion of apoptosis. LncRNA PURPL negatively regulated the miR-342-3p/PIK3R1 axis. In vivo results showed that the tumor volume and mass of si-PURPL group mice decreased compared to the si-NC group (P<0.05). Inhibiting miR-342-3p expression weakened the inhibitory effect of silencing LncRNA PURPL on tumor growth in vivo.
Conclusion:
Silting LncRNA PURPL can inhibit the malignant biological behavior of Huh-7 cells, and the mechanism may be related to the regulation of miR-342-3p/PIK3R1 axis.
2024 Vol. 30 (1): 29-35 [
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27
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Involvement of miRNA-93-5p in Cervical Cancer Progression by Regulating FBXW7 Gene
WANG Jiang
DOI: 10.3969/j.issn.1006-6233.2024.01.06
Objective:
To explore whether miRNA-93-5p can affect the progression of cervical cancer by regulating FBXW7 gene.
Methods:
Twenty clinical cervical cancer patient tissues were collected and divided into cancer-adjacent tissues and cervical cancer tissues. RT-qPCR was used to detect the levels of miRNA-93-5p and FBXW7 mRNA in cervical tissues. The miRNA-93-5p and FBXW7 mRNA levels were also measured in normal cervical epithelial cells (H8) and cervical cancer cells (Caski, HeLa, C33A). The cervical cancer cell line C33A was selected for further experiments. C33A cells were divided into the control group (normal culture), miRNA-93-5p inhibition group (transfected with low-expression miRNA-93-5p), and FBXW7 activation group (transfected with high-expression FBXW7). After 48 hours of transfection, cells were collected, and RT-qPCR was used to detect miRNA-93-5p and FBXW7 mRNA levels. Western blotting measured FBXW7 protein levels in C33A cells. Flow cytometry assessed apoptosis rates, plate colony assays examined cloning ability, scratch tests evaluated migration capacity, and Transwell assays measured invasion ability.
Results:
In comparison with cancer-adjacent tissues, cervical cancer tissues showed increased miRNA-93-5p mRNA levels and decreased FBXW7 mRNA levels (P<0.001). Compared with H8 cells, Caski, HeLa, and C33A cells exhibited increased miRNA-93-5p mRNA levels and decreased FBXW7 mRNA levels (P<0.05). Subsequent experiments were conducted using the C33A cervical cancer cell line. In the miRNA-93-5p inhibition group, miRNA-93-5p mRNA levels significantly decreased (P<0.001), apoptosis rates increased (P<0.001), cloning ability decreased (P<0.01), migration distance reduced (P<0.01), invasion ability decreased (P<0.01), and FBXW7 protein and mRNA levels significantly increased (P<0.01) compared with the control group. In the FBXW7 activation group, FBXW7 mRNA levels significantly increased (P<0.01), apoptosis rates increased (P<0.001), cloning ability decreased (P<0.001), migration distance reduced (P<0.01), invasion ability decreased (P<0.01) compared with the control group.
Conclusion:
miRNA-93-5p can affect the apoptosis, proliferation, migration and invasion of cervical cancer cells by regulating FBXW7 gene.
2024 Vol. 30 (1): 35-40 [
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25
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Establishment of a Guinea Pig Cholelithiasis Model Using Biliary Bacteria from Recurrent Common Bile Duct Stone Patients
BIAN Haoyu, HAN Ran, DUAN Xueguang, et al
DOI: 10.3969/j.issn.1006-6233.2024.01.07
Objective:
To establish a guinea pig cholelithiasis model using biliary bacteria from patients with recurrent common bile duct stones, providing a clinically relevant animal model for investigating the role of biliary microbiota in the recurrence of common bile duct stones and the formation of cholelithiasis.
Methods:
Thirty-five Dunkin Hartley guinea pigs, regardless of gender, were used in two stages of experiments (20 in the first stage and 15 in the second stage). Biliary bacteria from patients with recurrent common bile duct stones were introduced by biliary catheterization. In the first stage, observations were made at the 9th week post-modeling, including postoperative survival rate, bile characteristics, and intrahepatic and extrahepatic stone formation in the animal model. Bile samples were collected for bacterial culture. In the second stage, two animals were sacrificed daily from the 2nd day post-modeling to observe bile characteristics and stone formation in the biliary system. Pathological changes in the liver, gallbladder, and common bile duct tissues of gallstone-bearing animals were examined, and the feasibility of the modeling method for establishing a cholelithiasis animal model was comprehensively evaluated.
Results:
Compared to previous modeling methods using biliary catheterization with injection of Escherichia coli combined with incomplete obstruction, the method used in this study was more consistent with the biliary microbial status of patients with recurrent common bile duct stones during stone recurrence and cholelithiasis formation. Moreover, it had a shorter modeling period, higher model success rate, relatively lower operational difficulty, and lower animal mortality rate.
Conclusion:
The modeling method used in this study successfully established a guinea pig cholelithiasis model, providing a reliable animal model for studying the mechanisms underlying the role of biliary microbiota in the recurrence of common bile duct stones and the formation of cholelithiasis after ERCP procedures.
2024 Vol. 30 (1): 40-45 [
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27
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Evaluation of the Expression Level of miR-34b in Peripheral Blood for Targeted Treatment Effect and Prognosis in Stage Ⅲb-Ⅳ NSCLC Patients
ZHANG Yapeng, REN Yuchuan, LIU Zhao, et al
DOI: 10.3969/j.issn.1006-6233.2024.01.08
Objective:
To explore the evaluation value of peripheral blood microRNA-34b (miR-34b) for targeted therapy effect and prognosis in patients with stage Ⅲb-Ⅳ non-small cell lung cancer (NSCLC).
Methods:
A total of 104 NSCLC patients who received targeted treatment from January 2020 to January 2022 were selected. According to the efficacy of targeted treatment, they were divided into the effective group (n=40) and ineffective group (n=64). All patients were followed up for one year, and based on the prognosis, they were divided into the survival group (n=32) and death group (n=72). Real-time quantitative PCR was used to detect miR-34b in peripheral blood. The receiver operating characteristic curve (ROC) was employed to analyze the evaluation value of peripheral blood miR-34b expression level for the targeted treatment effect and prognosis of stage Ⅲb-Ⅳ NSCLC patients. Kaplan-Meier survival curve analysis was used to explore the relationship between different peripheral blood miR-34b expression levels and the prognosis of stage Ⅲb-Ⅳ NSCLC patients.
Results:
The peripheral blood miR-34b expression level in the effective group was higher than that in the ineffective group (P<0.05). The ROC curve showed that the AUC of peripheral blood miR-34b expression level in evaluating the targeted treatment effect of stage Ⅲb-Ⅳ NSCLC patients was 0.856 (P<0.05). The peripheral blood miR-34b expression level in the survival group was higher than that in the death group (P<0.05). The ROC curve showed that the AUC of peripheral blood miR-34b expression level in evaluating the prognosis of stage Ⅲb-Ⅳ NSCLC patients was 0.916 (P<0.05). The Log-rank test showed a statistically significant difference in survival rate between the miR-34b > 2.16 group and miR-34b ≤ 2.16 group (P<0.05).
Conclusion:
The expression level of peripheral blood miR-34b is associated with the targeted treatment effect and prognosis of stage Ⅲb-Ⅳ NSCLC patients. Detecting the expression level of peripheral blood miR-34b is helpful for evaluating the targeted treatment effect and prognosis.
2024 Vol. 30 (1): 45-49 [
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26
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The Expression Level and Significance of PCT Trx-1 and D-Lac in Patients with Sepsis before and after Continuous Blood Purification Treatment
ZENG Guangzhi, TANG Hailian, CHEN Haiyu, et al
DOI: 10.3969/j.issn.1006-6233.2024.01.09
Objective:
To investigate the changes of serum procalcitonin (PCT), thioredoxin (Trx-1) and D-lactic acid (D-Lac) levels before and after continuous blood purification (CBP) treatment in patients with sepsis, and to analyze its predictive value and clinical significance for the efficacy of CBP.
Methods:
A total of 100 patients with sepsis in our hospital from January 2021 to December 2022 were selected as the observation group, and 100 healthy subjects were selected as the control group according to the 1:1 pairing principle. The serum PCT, Trx-1 and D-Lac levels of the two groups were analyzed. Observation group received CBP treatment and was divided into survival subgroup and death subgroup according to treatment effect. The serum levels of PCT, Trx-1, D-Lac, acute physiology and chronic health status Ⅱ score (APACHEⅡ) were compared between different subgroups and the difference before and after treatment. Pearson correlation analysis was performed to assess the correlation between the changes in serum biomarker levels and APACHE II scores. Receiver operating characteristic curve (ROC) and area under curve (AUC) were used to analyze the predictive value of the difference in the level of each serum indicator. Kaplan-Meier was used to analyze the survival status of patients with different serum expressions within 28 days.
Results:
The serum levels of PCT, Trx-1 and D-Lac in observation group were higher than those in control group (P<0.05). After 1 week of treatment, the levels of serum PCT, Trx-1, D-Lac and APACHEⅡ score in death subgroup were higher than those in survival subgroup, and the difference was smaller than that in survival subgroup (P<0.05). There was a positive correlation between the difference of serum indexes and APACHEⅡ score (P<0.05). The AUC of the combined prediction of CBP was greater than that of the single prediction and the combined prediction of the serum prediction (P<0.05). The risk of death in patients with high expression of PCT, Trx-1 and D-Lac was 4.828, 3.600 and 2.318 times of that in patients with low expression, respectively, and the survival rate was higher than that in patients with low expression (P<0.05).
Conclusion:
The changes of serum PCT, Trx-1 and D-Lac levels in sepsis patients before and after CBP treatment can reflect the severity of the disease, and are closely related to the survival within 28 days. The combined detection of the changes has a certain predictive value for the efficacy of CBP.
2024 Vol. 30 (1): 50-55 [
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31
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Correlation Analysis Between Imaging Features Clinicopathological Characteristics and Molecular Subtypes of Non-Mass Ductal Carcinoma In Situ (DCIS)
LIU Fang, ZHOU Menghong, WANG Haijuan, et al
DOI: 10.3969/j.issn.1006-6233.2024.01.010
Objective:
To investigate the relationship between the imaging features and the clinicopathological characteristics of non-mass ductal carcinoma in situ (DCIS) and to analyze their correlation with molecular subtypes.
Methods:
This study selected 100 patients with non-mass DCIS who underwent surgical treatment at our hospital from June 2020 to June 2021.The patients were classified into four types (Type I:31 cases,Type Ⅱ:15 cases,Type Ⅲ:43 cases,and Type Ⅳ:11 cases) based on ultrasound findings.Immunohistochemistry was used to evaluate the positive expression of estrogen receptor (ER),progesterone receptor (PR),human epidermal growth factor receptor 2 (HER2),and Ki-67.Patients were categorized into Luminal A and Luminal B molecular subtypes according to the St.Gallen standard.
Results:
Type I exhibited thickened and tortuous ducts with low echogenicity within the lumen.Type Ⅱ displayed patchy hypoechoic areas with indistinct boundaries in the breast.Type Ⅱ showed lamellar hypoechoic areas with unevenly distributed punctate strong echoes in the breast.Type Ⅳ presented disordered glandular structures with distorted architecture.There were no statistically significant differences in age,tumor diameter,menstrual status,lesion location,and clinical symptoms among patients with Type I,Type Ⅱ,Type Ⅲ,and Type Ⅳ non-mass DCIS (P>0.05).However,as the ultrasound classification level increased,nuclear grade also increased (P<0.05).There were no significant differences in the positive expression of ER,PR,Ki-67 levels among Type I,Type Ⅱ,Type Ⅲ and Type Ⅳ non-mass DCIS patients (P>0.05).But,there was a significant difference in HER2 positive expression between Type I and Type Ⅱ,with Types Ⅲ and Ⅳ showing a higher HER2 positive expression (P<0.05).There was no significant difference in HER2 positive expression between type I and type II patients (P>0.05). Compared to type I, type III and type IV showed higher HER2 positive expression (P<0.05). Type III had higher HER2 positive expression compared to type II, but the difference with type IV was not statistically significant (P>0.05). The distribution of Luminal A and Luminal B subtypes increased in type III compared to type I (P<0.05), and the distribution of Luminal B subtype increased in type IV compared to type I (P<0.05), while the difference in Luminal A subtype was not statistically significant (P>0.05).
Conclusion:
Patients with non-mass DCIS mainly exhibited calcification on ultrasound.Patients with structural disorders had higher nuclear grades and more HER2 positive expression.These factors were correlated with a decrease in Luminal A subtype and an increase in Luminal B subtype.
2024 Vol. 30 (1): 55-60 [
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Comparison of Effect and Safety of AOPT with Different Band Filters in the Treatment of Facial Telangiectasia
LI Wei, ZHU Lixia, DANG Hui, et al
DOI: 10.3969/j.issn.1006-6233.2024.01.011
Objective:
To investigate the effectiveness and safety of using Advanced Optimal Pulse Technology (AOPT) with different wavelength band filters in the treatment of facial telangiectasia (FT).
Methods:
Clinical data from 128 FT patients were collected and they were randomly divided into a control group and an observation group,each consisting of 64 patients.Both groups received AOPT treatment,with the control group using a vascular filter with a wavelength of 560~1200nm,and the observation group using vascular filters with wavelengths of 530~650nm and 900~1200nm.The clinical efficacy of the two groups was assessed after 5 months of treatment and at a 3-month post-surgery follow-up.The facial aesthetic zone score and capillary morphology score were compared before treatment,after 5 months of treatment,and at 3 months after surgery.Postoperative adverse reactions in both groups were observed,and the satisfaction with facial repair at the 3-month post-surgery follow-up was investigated.
Results:
The total clinical effectiveness rates after treatment and at the 3-month post-surgery follow-up in the observation group were 93.75% and 100.00%,significantly higher than the 75.00% and 90.63% in the control group (P<0.05).There were statistically significant effects of time and between-group differences in facial aesthetic zone scores (P<0.05),and the capillary morphology score showed a significant effect over time (P<0.05).Both groups had reduced facial aesthetic zone scores and capillary morphology scores compared to before surgery,with the scores in the control group being higher than those in the observation group (P<0.05).The total incidence of postoperative adverse reactions in the observation group was 1.56%,significantly lower than the 12.50% in the control group (P<0.05).The facial repair satisfaction in the observation group was 100.00% at the 3-month post-surgery follow-up,significantly higher than the 93.75% in the control group (P<0.05).
Conclusion:
The use of AOPT technology with vascular filters having wavelengths of 530-650nm and 900-1200nm in the treatment of FT patients provides clear short-term and long-term efficacy.This treatment significantly improves facial aesthetics and capillary morphology,reduces postoperative adverse reactions,and enhances patient satisfaction with facial repair.
2024 Vol. 30 (1): 61-65 [
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Analysis of the Impact of MTHFR C677T Gene Polymorphism-Hcy Interaction on Hypertension Complicated with Coronary Heart Disease Using Multifactor Dimensionality Reduction
ZHANG Mingchao, WANG Juan, LU Dasheng, et al
DOI: 10.3969/j.issn.1006-6233.2024.01.012
Objective:
To analyze the effect of methylene tetrahydrofolate reductase (MTHFR) C677T gene polymorphism-Hcy interaction on hypertension combined with coronary heart disease based on multifactor dimension reduction (GMDR).
Methods:
A total of 150 hypertensive patients admitted to our hospital from March 2021 to October 2022 were selected as the study subjects. Based on coronary angiography results, they were divided into the isolated hypertension group (n=51) and the hypertension complicated with coronary heart disease group (n=99). General information, Hcy levels, and MTHFR C677T gene polymorphism distribution were compared between the two groups. Logistic regression analysis was used to identify factors influencing hypertension complicated with coronary heart disease, and GMDR was employed to analyze the interaction between MTHFR C677T gene polymorphism and Hcy.
Results:
There was no statistically significant difference between the observed and theoretical values of MTHFR C677T genotypic frequencies in the two groups (P>0.05). The duration of hypertension, Hcy, LDL-C, MTHFR C677T genotypes (TT), and the T allele were identified as influencing factors for hypertension complicated with coronary heart disease (P<0.05). GMDR results showed that the TT genotype of MTHFR C677T in interaction with Hcy formed the optimal model for the risk of hypertension complicated with coronary heart disease. The cross-validation consistency was 10/10, the testing accuracy was 75.00%, and the sign test P-value was 0.011.
Conclusion:
MTHFR C677T gene polymorphism and the interaction with Hcy may increase the risk of coronary heart disease. Clinicians should dynamically monitor changes in these indicators and take effective treatment measures promptly to reduce the occurrence of coronary heart disease.
2024 Vol. 30 (1): 65-70 [
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Effects of Continuous Blood Purification and Intermittent Hemodialysis on Serum RBP4 Tf and β
2
-MG Levels in Elderly Patients with Acute Renal Failure
HE Li, ZHANG Fan, CHENG Caijuan, et al
DOI: 10.3969/j.issn.1006-6233.2024.01.013
Objective:
To explore the effects of continuous blood purification (CBP) and intermittent hemodialysis (IHD) on levels of serum retinol-binding protein 4 (RBP4), transferrin (Tf) and β
2
-microglobulin (β
2
-MG) in elderly patients with acute renal failure (ARF).
Methods:
A total of 114 ARF patients admitted to our hospital from January 2021 to January 2023 were included in this study. The patients were randomly divided into two groups using a random number table, with 57 cases in each group (observation group and control group). The control group received IHD, and the observation group received CBP, with a dialysis period of 2 weeks for both groups. Clinical efficacy, serum levels [RBP4, Tf, β
2
-MG, Serum Creatinine (Scr), and Blood Urea Nitrogen (BUN)] before and 2 weeks after dialysis, as well as electrolyte indicators (K
+
, Cl
-
, Na
+
), were compared between the two groups. Complications during the treatment were recorded.
Results:
After dialysis, the total effective rates in the observation group and control group were 94.74% (54/57) and 78.95% (45/57), respectively, with a statistically significant difference (P<0.05). After 2 weeks of dialysis, the serum levels of RBP4, Tf, β
2
-MG, Scr, and BUN in both groups decreased significantly compared to before dialysis, and the reduction in the observation group was greater than that in the control group (P<0.05). After 2 weeks of dialysis, the serum levels of K
+
, Cl
-
, and Na
+
in both groups increased significantly compared to before dialysis, and the increase in the observation group was greater than that in the control group (P<0.05). The incidence of complications during dialysis in both groups (5.26%, 7.02%) showed no significant difference (P>0.05).
Conclusion:
CBP is more effective in elderly ARF patients, effectively removing small and large molecular toxins from the blood and maintaining electrolyte stability.
2024 Vol. 30 (1): 71-75 [
Abstract
] (
36
)
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76
Impact of Laparoscopic Anterior Resection for Rectal Cancer with Preservation of the Left Colic Artery on Gastrointestinal Function and Postoperative Complications
JU Ran, LIU Ping, MIAO Qi
DOI: 10.3969/j.issn.1006-6233.2024.01.014
Objective:
To investigate the effects of laparoscopic anterior resection (LAR) with preservation of the left colic artery (LCA) on gastrointestinal function and postoperative complications in patients with rectal cancer.
Methods:
A retrospective analysis was conducted on the clinical data of 105 patients who underwent LAR in our hospital from January 2019 to January 2022. Based on the treatment of LCA, patients were divided into the preservation group (54 cases, preserving LCA) and the non-preservation group (51 cases, not preserving LCA). Surgical parameters, lymph node dissection, preoperative and postoperative 2-day gastrointestinal function [gastrin (GAS), motilin (MTL)], and anal function [Wexner constipation score (WCS)] were compared between the two groups. The occurrence of complications in both groups was also recorded.
Results:
The preservation group had a longer operation time than the non-preservation group, and the incidence of complications was lower in the preservation group (both P < 0.05). There were no significant differences in blood loss, extubation time, postoperative drainage, length of hospital stay, lymph node dissection, GAS, MTL levels, and WCS scores between the two groups (all P > 0.05).
Conclusion:
Preserving the LCA during LAR for rectal cancer does not affect lymph node dissection, gastrointestinal function, or anal function. Moreover, it may reduce the incidence of postoperative complications.
2024 Vol. 30 (1): 76-80 [
Abstract
] (
30
)
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38
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80
Clinical Value of Platelet Associated Antibodies in Evaluating the Therapeutic Effect on Children with Immune Thrombocytopenia
LIU Na, BAI Taomin, LI Rui, et al
DOI: 10.3969/j.issn.1006-6233.2024.01.015
Objective:
To investigate the clinical value of platelet associated antibodies (PA-Ig) in evaluating the therapeutic effect on children with immune thrombocytopenia (ITP).
Methods:
A study from February 2020 to February 2023 was conducted. 104 children with ITP (observation group) and 22 healthy children (control group) were selected as the study subjects. Children in the observation group were divided into the complete response group, the effective group, and the ineffective group based on the treatment outcomes. All children were tested for PA-Ig levels.
Results:
Among the 104 children in the observation group, 85 (81.73%) achieved complete response (complete response group), 11 (10.58%) showed effectiveness (effective group), and the remaining 8 (7.69%) were ineffective (ineffective group). PA-IgG, PA-IgM, and PA-IgA levels in the observation group were lower than those in the control group (P<0.05). In the complete response group, PA-IgG, PA-IgM, and PA-IgA levels were lower than in the effective and ineffective groups. The effective group had lower levels compared to the ineffective group. There were statistically significant differences in PA-IgG, PA-IgM, and PA-IgA levels among the three groups with different treatment outcomes (P<0.05).
Conclusion:
Testing PA-Ig levels in pediatric ITP patients is beneficial for tailoring treatment and assessing early efficacy, reducing the risk of treatment, and has certain value in the evaluation of treatment outcomes in pediatric ITP patients.
2024 Vol. 30 (1): 80-83 [
Abstract
] (
40
)
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83
The Value of Serum FT3/FT4 IGFBP7 and NT proBNP in Predicting Poor Prognosis in AHF Patients
LEI Ronghao, XIAO Baozhu, PAN Wan, et al
DOI: 10.3969/j.issn.1006-6233.2024.01.016
Objective:
To explore the value of the free triiodothyronine/thyroxine ratio (FT3/FT4), insulin-like growth factor-binding protein7 (IGFBP7), and N-terminal pro-brain natriuretic peptide (NT-proBNP) in predicting adverse outcomes of patients with acute heart failure (AHF).
Methods:
A total of 125 AHF patients treated at our hospital from February 2020 to February 2022 were selected. A 6-month follow-up survey was conducted, and patients were categorized into two groups based on whether adverse events such as death or recurrent heart failure occurred during the follow-up: a good prognosis group (n=44) and a poor prognosis group (n=81). General information was collected for both groups, and the FT3/FT4, IGFBP7, and NT-proBNP levels at admission were analyzed to explore their predictive value for adverse outcomes in AHF patients.
Results:
At admission, the FT3/FT4 levels in the poor prognosis group were lower than those in the good prognosis group, while IGFBP7 and NT-proBNP levels were higher (P<0.05). Univariate analysis revealed that age, use of β-blockers, and diuretics had an impact on patient prognosis (P<0.05). Multivariate logistic regression analysis using patient prognosis as the dependent variable (good prognosis=0, poor prognosis=1) showed that low levels of FT3/FT4 and high levels of IGFBP7 and NT-proBNP may affect adverse outcomes in patients (P<0.05). ROC curve analysis explored the predictive value of FT3/FT4, IGFBP7, and NT-proBNP levels for adverse outcomes, with AUC values of 0.899, 0.913, and 0.878, and optimal cutoff values of 69.88, 336.93, and 0.235, respectively (P<0.05).
Conclusion:
Serum FT3/FT4, IGFBP7, and NT-proBNP have a certain predictive value for adverse outcomes in AHF patients and can be applied in clinical practice.
2024 Vol. 30 (1): 83-88 [
Abstract
] (
35
)
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88
Diagnostic Value of Combined Serum PTX3 SFRP4 and Urinary NGAL in Diabetic Nephropathy
WANG Wenyi, YU Jingtian, WANG Jielian
DOI: 10.3969/j.issn.1006-6233.2024.01.017
Objective:
To explore the diagnostic significance of combined detection of neutrophil gelatinase-associated lipocalin (NGAL) in urine, serum pentraxin 3 (PTX3), and secreted frizzled-related protein 4 (SFRP4) in clinical diabetic nephropathy (DN).
Methods:
A total of 86 DN patients treated at our hospital from December 2020 to June 2023 were selected as the DN group. According to urinary albumin excretion rate (UAER), they were divided into the early group (24 cases, UAER ≤ 20 μg/min), the middle group (30 cases, 20 μg/min < UAER < 200 μg/min), and the late group (32 cases, UAER ≥ 200 μg/min). Based on the severity of the disease, they were further divided into mild DN group (40 cases) and moderate to severe DN group (46 cases). Additionally, 40 patients with type 2 diabetes without nephropathy were selected as the control group. Serum PTX3, SFRP4, and urinary NGAL levels were compared between the DN group and the control group, among different severity groups, and between early, middle, and late-stage groups. The clinical value of the three markers and their combined detection in diagnosing DN was analyzed.
Results:
Levels of serum PTX3, SFRP4, and urinary NGAL in the DN group were higher than those in the control group (P < 0.05). Patients in the moderate to severe DN group showed significantly elevated levels of all three markers compared to the mild DN group (P < 0.05). The levels of the three markers in the middle and late-stage DN groups were higher than those in the early-stage group (P < 0.05). ROC curve analysis showed that the area under the curve (AUC) for the combined diagnosis of DN by the three markers was 0.985, and the AUC for the combined diagnosis was higher than that for individual markers (Z combined-PTX3=2.670, Z combined-SFRP4=3.976, Z combined-NGAL=2.510, P=0.008, 0.000, 0.012). The sensitivity was 94.20%, and the specificity was 96.00%.
Conclusion:
Combined detection of serum PTX3, SFRP4, and urinary NGAL has higher reliability in diagnosing DN and assessing the severity of DN patients.
2024 Vol. 30 (1): 88-92 [
Abstract
] (
53
)
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93
Changes in Peripheral Blood NLR PLR and HGB Levels in Maintenance Peritoneal Dialysis Patients and Their Relationship with Calcium-Phosphorus Metabolism Abnormalities and All-Cause Mortality
MEI Jiben, HE Jing, CHEN Zhonghui, et al
DOI: 10.3969/j.issn.1006-6233.2024.01.018
Objective:
To explore the changes in peripheral blood neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and hemoglobin (HGB) levels in maintenance peritoneal dialysis (PD) patients and their relationship with calcium-phosphorus metabolism abnormalities and all-cause mortality.
Methods:
A total of 206 maintenance PD patients admitted from March 2019 to June 2022 were selected. Patients were assessed for the presence of calcium-phosphorus metabolism abnormalities and divided into abnormal and normal groups. Peripheral blood NLR, PLR, and HGB levels were compared between the two groups. The correlation between NLR, PLR, HGB, and blood calcium and phosphorus levels was analyzed. Follow-up was conducted until December 2022, with all-cause mortality as the endpoint event. Patients were divided into the death group and the survival group, and peripheral blood NLR, PLR, and HGB levels were compared. Receiver operating characteristic (ROC) curves were used to evaluate the predictive value of NLR, PLR, and HGB for all-cause mortality.
Results:
In the abnormal group, peripheral blood NLR, PLR, and blood phosphorus levels were higher, while blood calcium levels were lower than those in the normal group (P<0.05). However, there were no statistically significant differences in age, gender, education level, primary disease, dialysis time, and peripheral blood HGB levels between the two groups (P>0.05). In elderly patients undergoing maintenance hemodialysis, peripheral blood NLR and PLR were negatively correlated with blood calcium levels and positively correlated with blood phosphorus levels (P<0.05). However, HGB showed no significant correlation with blood calcium and phosphorus levels (P>0.05). The dialysis time in the death group was longer than that in the survival group. Peripheral blood NLR, PLR, and blood phosphorus levels were higher, while blood calcium levels were lower in the death group compared to the survival group (P<0.05). However, there were no statistically significant differences in age, gender, education level, primary disease, and peripheral blood HGB levels between the two groups (P>0.05). ROC curve analysis showed that the areas under the curve (AUC) for peripheral blood NLR and PLR in predicting all-cause mortality were 0.777 (95% CI: 0.6880.895), respectively.
Conclusion:
Calcium-phosphorus metabolism abnormalities in maintenance peritoneal dialysis patients are related to peripheral blood NLR and PLR levels. These indicators have a certain predictive value for all-cause mortality in patients.
2024 Vol. 30 (1): 93-98 [
Abstract
] (
37
)
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98
Analysis of Peripheral Blood Immune Indices in Patients with Bronchiectasis Complicated by NTM Infection
MEI Yizhi, YAO Chao, ZHANG Biling
DOI: 10.3969/j.issn.1006-6233.2024.01.019
Objective:
To analyze the level of peripheral blood immune spectrum in patients with bronchiectasis complicated with nontuberculous Mycobacterium (NTM).
Methods:
Ninety-six patients with bronchiectasis admitted to our hospital from May 2021 to May 2023 were selected. The patients were divided into an infection group (33 cases) and a non-infection group (63 cases) based on whether NTM infection was present. In the infection group, patients were further categorized based on the number of lung lobes affected by bronchiectasis: 1~2 lobes group (11 cases), 3~4 lobes group (12 cases), and 5~6 lobes group (10 cases). Peripheral blood levels of immunoglobulins, T lymphocyte subsets, high-sensitivity C-reactive protein (hs-CRP), cystatin C (CysC), and homocysteine (Hcy) were compared between the groups.
Results:
There was no significant difference in the levels of peripheral blood immunoglobulins between the two groups (P>0.05). The peripheral blood CD3
+
and CD4
+
levels were lower, and CD8
+
levels were higher in the infection group compared to the non-infection group (P<0.05). There was no significant difference in peripheral blood Hcy levels between the two groups (P>0.05). However, hs-CRP and CysC levels were higher in the infection group than in the non-infection group (P<0.05). When comparing different groups based on the number of affected lung lobes, there was no significant difference in peripheral blood IgA, IgM, IgG, CD8
+
levels (P>0.05). However, the CD3
+
and CD4
+
levels showed a decreasing trend from the 1~2 lobes group to the 5~6 lobes group (P<0.05). There was no significant difference in peripheral blood hs-CRP, CysC, and Hcy levels among the three groups (P>0.05).
Conclusion:
In patients with bronchiectasis complicated by NTM infection, peripheral blood CD3
+
and CD4
+
levels are decreased, CD8
+
, hs-CRP, and CysC levels are increased. Furthermore, CD3
+
and CD4
+
levels are correlated with the number of affected lung lobes.
2024 Vol. 30 (1): 98-102 [
Abstract
] (
36
)
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44
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102
Efficacy Observation of Zishui Qingxin Formula in the Treatment of Insomnia in Menopausal Women
QUAN Xingmiao, LIU Yulan, WANG Yue, et al
DOI: 10.3969/j.issn.1006-6233.2024.01.020
Objective:
To observe and analyze the clinical effect of Zishui Qingxin Formula on menopause insomnia.
Methods:
Eighty menopausal women with insomnia admitted to our hospital from March 2017 to December 2019 were randomly divided into a control group (40 patients) and a treatment group (40 patients). The control group received conventional treatment, while the treatment group received Zishui Qingxin Formula in addition to the control group. After treatment, the traditional Chinese medicine syndrome scores, weekly sleep quality (PSQI), and polysomnography (PSG) parameters were observed and compared between the two groups.
Results:
After treatment, TCM syndrome score, PSQI, PSG parameters (wake times, wake time, sleep latency, fast wave sleep latency) were lower than before treatment (P<0.05), PSG parameters (sleep efficiency, fast wave sleep, slow wave sleep) were higher than before treatment (P<0.05), and the treatment group was better than the control group (P<0.05). The total effective rate of the treatment group (95%) was higher than that of the control group (77.5%) (P<0.05).
Conclusion:
Zishui Qingxin Formula is effective in the treatment of menopausal insomnia patients, and can effectively improve insomnia symptoms and sleep quality.
2024 Vol. 30 (1): 102-106 [
Abstract
] (
50
)
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106
Effect of Total Thyroidectomy by Total Endoscopic Areola Approach on Parathyroid Function in the Treatment of Thyroid Papillary Carcinoma
HOU Yufei, AO Yazhou, QI Yantao
DOI: 10.3969/j.issn.1006-6233.2024.01.021
Objective:
To investigate the impact of transareolar endoscopic thyroidectomy on parathyroid function in the treatment of papillary thyroid carcinoma.
Methods:
Clinical data of 130 patients with bilateral micro papillary thyroid carcinoma who underwent total thyroidectomy from March 2020 to January 2023 at the Affiliated Hospital of Chengde Medical College were collected and retrospectively analyzed. Among them, 30 cases underwent total thyroidectomy with central lymph node dissection through the transareolar route, and 100 cases underwent total thyroidectomy with central lymph node dissection through the traditional neck approach. Serum parathyroid hormone and blood calcium levels were compared between the two groups before surgery and at 1 day, 7 days, 1 month, 3 months, and 6 months after surgery.
Results:
There was no statistically significant difference in serum parathyroid hormone and blood calcium levels between the two groups before surgery, at 1 day, 7 days, 3 months, and 6 months after surgery (P>0.05). At 1 month after surgery, there was a statistically significant difference in serum parathyroid hormone and blood calcium levels between the two groups (P<0.05), with a greater decrease in the transareolar surgery group (P<0.05). Calcium supplementation was given orally or intravenously after surgery. After 6 months, 4 cases in the transareolar approach group had serum parathyroid hormone levels <15 pg/mL, while 11 cases in the traditional approach group had levels <15 pg/mL. After 6 months, 2 cases in the transareolar approach group had blood calcium levels <2.0 mmoL/L, while 4 cases in the traditional neck approach group had levels <2.0 mmoL/L.
Conclusion:
For total thyroidectomy, there was no statistically significant difference in the incidence of permanent parathyroid dysfunction between transareolar endoscopic thyroidectomy and traditional open neck approach. For patients with cosmetic needs, transareolar endoscopic thyroidectomy is a safe and effective surgical approach.
2024 Vol. 30 (1): 106-110 [
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] (
69
)
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111
Value of Combined Ultrasonic NT and LC with Maternal Blood Molecular Markers in Assessing Pregnancy Outcomes in High-Risk Pregnant Women
CHEN Gaojin, ZHANG Tongxi, FENG Fei
DOI: 10.3969/j.issn.1006-6233.2024.01.022
Objective:
To explore the value of combined measurement of nuchal translucency (NT), cervical length (LC), free beta-human chorionic gonadotropin (B-HCG), pregnancy-associated plasma protein-A (PAPPA), and D-dimer (D-D) in assessing pregnancy outcomes in high-risk pregnant women.
Methods:
A retrospective study included 80 high-risk pregnant women who visited our hospital from January 2019 to January 2022. Participants were categorized into groups with adverse pregnancy outcomes and those without. The differences in NT, LC, arterial S/D ratio, PI, RI, serum B-HCG, PAPPA, and D-D levels were compared between the two groups. The correlation between these indicators and their predictive value for adverse pregnancy outcomes were analyzed.
Results:
Pregnant women with adverse pregnancy outcomes had significantly higher levels of B-HCG, PAPPA, D-D, and NT (2.28±0.62 mom, 0.72±0.16 mom, 351.12±82.80 mg/L, and 3.78±0.81 mm, respectively) compared to those without adverse outcomes (1.75±0.41 mom, 0.57±0.15 mom, 295.33±85.52 mg/L, and 3.31±0.64 mm, respectively) (P<0.01). LC was significantly lower in women with adverse outcomes (24.49±4.53 mm) compared to those without adverse outcomes (31.01±5.12 mm) (P<0.01). B-HCG, PAPPA, and D-D levels were positively correlated with NT (P<0.05), while negatively correlated with LC (P<0.05). The combined use of NT, LC, B-HCG, PAPPA, and D-D in predicting adverse pregnancy outcomes in high-risk pregnant women had an AUC of 0.911 (95% CI: 0.848–0.974), significantly higher than individual indicators. The sensitivity and specificity were 79.20% and 96.30%, respectively (P<0.01).
Conclusion:
NT, LC, B-HCG, PAPPA, and D-D are correlated with adverse pregnancy outcomes in high-risk pregnant women, and their combined use has good predictive value for adverse pregnancy outcomes in high-risk pregnancies.
2024 Vol. 30 (1): 111-115 [
Abstract
] (
45
)
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115
Effects of 3D Smart mA Modulation Technology on Image Acquisition Time, Quality and Radiation Dose of Patients with Different BMI
YANG Huiling, ZHANG Shuo, ZHAO Wenzhe, et al
DOI: 10.3969/j.issn.1006-6233.2024.01.023
Objective:
To analyze the effects of 3D smart mA modulation technology on image acquisition time, quality and radiation dose of patients with different body mass index (BMI).
Methods:
A total of 180 patients undergoing chest CT scans at The First Affiliated Hospital of Xi'an Jiaotong University from June 2021 to December 2022 were selected. Patients were divided into three groups based on BMI: Group A (18.5 kg/m
2
≤ BMI ≤ 23.9 kg/m
2
, n=75), Group B (23.9 kg/m
2
< BMI ≤ 26.9 kg/m
2
, n=62), and Group C (26.9 kg/m
2
< BMI ≤ 30.5 kg/m
2
, n=43). All patients used 3D smart mA modulation technology, and the image acquisition time, image quality, and radiation dose were compared among the three groups.
Results:
There was no statistically significant difference in image acquisition time among the three groups (F=2.573, P>0.05). Two physicians evaluated the image quality scores (IQS) of different pulmonary parts, with high consistency (Kappa values=0.768, 0.812, 0.861). There was no statistically significant difference in the IQS scores for different pulmonary parts among the three groups (P>0.05). There were statistically significant differences in CT values of different pulmonary parts among the three groups, and the values decreased with the increase of BMI (P<0.05). There was no statistically significant difference in the standard deviation (SD) of different pulmonary parts, or volume CT dose index (CTDIvol) among the three groups (P>0.05). DLP and ED increased in order from group A, group B to group C (P<0.05).
Conclusion:
Applying 3D smart mA modulation to patients with different BMI can effectively reduce radiation dose on the premise of ensuring image quality.
2024 Vol. 30 (1): 115-120 [
Abstract
] (
35
)
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120
Effect and Impact on Adjacent Teeth Relationship and Occlusion of Porcelain High Inlay Restoration in the Treatment of Elderly Permanent Tooth Crown Defects
WU Song, YE Huaiguang
DOI: 10.3969/j.issn.1006-6233.2024.01.024
Objective:
To explore the effectiveness of porcelain high inlay restoration in the treatment of crown defects in elderly permanent teeth and its impact on the restoration of adjacent teeth relationship and occlusion.
Methods:
A retrospective analysis was conducted on the clinical data of 116 elderly patients (116 affected teeth) with crown defects treated at the People's Hospital of Guang'an from September 2020 to October 2022. Based on different restoration materials, patients were divided into the resin group (57 cases, 57 affected teeth) and the porcelain high inlay group (59 cases, 59 affected teeth). The resin group underwent direct resin filling, while the porcelain high inlay group underwent porcelain high inlay restoration. Six months after restoration, the restoration effects, aesthetic outcomes, occlusion conditions immediately after restoration and 6 months later, and periodontal health (gingival index [GI], plaque index [PLI], gingival bleeding index) before and after restoration were compared between the two groups.
Results:
Six months after restoration, the porcelain high inlay group showed higher USPHS satisfaction, aesthetic scores, and percentage of centric occlusion on the affected side than the resin group (P<0.05). The gingival bleeding index, GI, and PLI levels were lower than those in the resin group (P<0.05).
Conclusion:
Porcelain high inlay restoration has a significant effect in the treatment of crown defects in elderly permanent teeth, and it is superior to direct resin filling in terms of aesthetic outcomes, occlusal function, and periodontal health.
2024 Vol. 30 (1): 120-124 [
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39
)
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124
Morphological Differences of Colorectal Polyps Under NBI Endoscopy and Their Diagnostic Value in Polyp Characterization
CAI Ping, SONG Zhi, YANG Qian, et al
DOI: 10.3969/j.issn.1006-6233.2024.01.025
Objective:
To investigate the morphological differences of colorectal polyps under Narrow Band Imaging (NBI) endoscopy and evaluate their diagnostic value in distinguishing the nature of polyps.
Methods:
A total of 126 patients (768 polyps) diagnosed with colorectal polyp-like lesions during colonoscopy from February 2021 to February 2023 at the First Hospital of Qiqihar were included. The size, morphology, and surface mucosa of polyps were analyzed. Polyps were classified into non-neoplastic polyps and adenomas based on pathological results. The diagnostic value of the NICE classification in determining polyp nature was calculated, and ROC curves were plotted.
Results:
The proportion of polyps with a diameter ≥1.0 cm in high-grade epithelial dysplasia adenomas was significantly higher than in tubular adenomas, inflammatory polyps, and hyperplastic polyps (P<0.003). The proportion of polyps with a diameter ≥1.0 cm in tubular-villous adenomas was significantly higher than in inflammatory polyps and hyperplastic polyps (P<0.003). The proportion of polyps with a diameter ≥1.0 cm in tubular-villous adenomas was significantly higher than in inflammatory polyps (P<0.003). The Yamada classification of high-grade epithelial dysplasia adenomas differed significantly from that of villous adenomas and hyperplastic polyps (P<0.003). The proportion of lobulated polyps in villous adenomas was significantly higher than in tubular adenomas, high-grade epithelial dysplasia adenomas, inflammatory polyps, tubular-villous adenomas, and hyperplastic polyps (P<0.003). The percentage of polyps with smooth mucosal surfaces in tubular adenomas, inflammatory polyps, tubular-villous adenomas, and hyperplastic polyps was significantly higher than in villous adenomas and high-grade epithelial dysplasia adenomas (P<0.003). The sensitivity, specificity, missed diagnosis rate, and misdiagnosis rate of NICE classification in determining the nature of colorectal polyps were 88.62%, 90.94%, 11.38%, and 9.06%, respectively.
Conclusion:
Under NBI, there are significant differences in the morphological characteristics, size and surface mucosal characteristics of colorectal polyps in different pathological types, and NBI examination has a high clinical application value in the differential diagnosis of colorectal polyps.
2024 Vol. 30 (1): 124-129 [
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] (
23
)
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130
Diagnostic Value of Blood Viscosity Reticulated Platelets and Platelet Parameters in Elderly Immune Thrombocytopenia
YANG Qiuting, ZHU Yanjun, FAN Yongli
DOI: 10.3969/j.issn.1006-6233.2024.01.026
Objective:
To explore the diagnostic value of blood viscosity, reticulated platelets, and platelet parameters in elderly patients with immune thrombocytopenia (ITP).
Methods:
A total of 82 elderly (age 60 and above) ITP patients from our hospital between 2020 and 2022 were included as the observation group. The control group included 50 elderly patients with non-immune thrombocytopenia (NITP) (25 cases with cirrhosis, 15 cases with hematological diseases, and 10 cases with other tumors). Another 50 elderly health examination participants were included as the second control group. Blood samples were collected for blood rheology analysis, including blood viscosity using a hemorheology analyzer, and platelet parameters [platelet distribution width (PDW), mean platelet volume (MPV), platelet-large cell ratio (P-LCR)] using an automated hematology analyzer. Comparative analysis of blood viscosity, reticulated platelets, PDW, MPV, and P-LCR before and after treatment was performed.
Results:
Before treatment, the observation group showed higher PDW, MPV, and reticulated platelet ratio compared to both control groups, while blood viscosity was lower, with statistical significance (P<0.05). There was no significant difference between Control Group 1 and Control Group 2 (P>0.05). As the platelet count decreased in the observation group, PDW and MPV increased, and blood viscosity decreased, showing statistically significant differences between the observation group and the control groups (P<0.05). Comparative analysis before and after treatment showed that in the observation group, the blood viscosity and platelet count of severe and mild-to-moderate patients increased after treatment (P<0.05), while reticulated platelets, P-LCR, PDW, and MPV significantly decreased and were lower than before treatment, with statistical significance (P<0.05).
Conclusion:
Determining blood viscosity, reticulated platelets, and platelet parameters significantly contributes to the etiological diagnosis and disease assessment of elderly ITP patients. Additionally, it plays a positive role in prognosis evaluation, providing ample diagnostic support for clinical practice.
2024 Vol. 30 (1): 130-134 [
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38
)
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135
Occurrence of Metabolic Syndrome in Infertile Patients with Polycystic Ovary Syndrome (PCOS) and Its Relationship with Ovulation Induction Outcomes
ZOU Xuan, LI Jin, JIANG Tao
DOI: 10.3969/j.issn.1006-6233.2024.01.027
Objective:
To explore the occurrence of metabolic syndrome (MS) and its relationship with ovulation induction outcomes in infertility patients with polycystic ovary syndrome (PCOS).
Methods:
A total of 225 infertile PCOS patients admitted from January 2020 to April 2023 were selected as the study subjects, and the occurrence of MS was recorded. Based on whether MS occurred or not, patients were divided into the MS group and the non-MS group. Clinical data were compared between the two groups, and the relationship between MS and ovulation induction outcomes was analyzed.
Results:
Among the 225 PCOS infertile patients, 31 patients had concomitant MS, accounting for 13.78%. In the MS group, BMI (≥24 kg/m
2
), waist circumference (≥88cm), systolic blood pressure (≥130 mmHg), diastolic blood pressure (≥85 mmHg), FPG (≥126 mg/dL), TG (≥150 mg/dL), FINS, HOMA-IR levels were higher than those in the non-MS group, while HDL-C (≥59 mg/dL) level was lower than the non-MS group (P<0.05). The ovulation rate and clinical pregnancy rate in the MS group were lower than those in the non-MS group (χ
2
=6.745; χ
2
=5.292, P<0.05). Binary logistic analysis showed that age (≥30 years), waist circumference (≥88cm), MS (yes), FINS, HOMA-IR, estradiol (E2), testosterone (T), anti-Müllerian hormone (AMH) were independent risk factors affecting ovulation induction outcomes in PCOS infertile patients (P<0.05).
Conclusion:
The occurrence of MS is relatively high in PCOS infertile patients, and it is correlated with ovulation induction outcomes. Ovulation induction outcomes are influenced by factors such as age, waist circumference, MS, FINS, HOMA-IR, E2, T, AMH.
2024 Vol. 30 (1): 135-141 [
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Correlation Analysis of Gut Microbiota Metabolites Short-Chain Fatty Acids (SCFAs) and Skeletal Muscle Mass in Healthy Children Aged 6~9
LONG Haixu, ZHAN Hongyan, LU Ying, et al
DOI: 10.3969/j.issn.1006-6233.2024.01.028
Objective:
To explore the correlation between gut microbiota metabolites, specifically short-chain fatty acids (SCFAs), and skeletal muscle mass in healthy children aged 6-9.
Methods:
This cross-sectional study was conducted from December 2021 to January 2023. A total of 176 participants (68 girls and 108 boys) were included in the study. Skeletal muscle mass indices were measured using dual-energy X-ray absorptiometry (DXA), and participants were stratified based on bone mineral density (BMD) values. The concentration of SCFAs in feces was determined using high-performance liquid chromatography (HPLC). Spearman correlation analysis was performed to assess the relationship between fecal SCFAs and skeletal muscle mass.
Results:
Based on TBLH bone density reference values, children included in the study were classified into normal BMD (Z score ≥ 0, n = 120) and low BMD (Z score < 0, n = 56) groups. Compared to the normal BMD group, the low BMD group showed significantly lower TB BMD, TBLH BMD, LS BMD, PELV BMD, TSM/TBF, ASM/AFM (P<0.05), and significantly increased TSM, ASM (P<0.05). The low BMD group had a significantly higher level of fecal butyric acid and significantly lower levels of acetic acid, propionic acid, total SCFAs compared to the normal BMD group (P<0.05). After adjusting for covariates and false discovery rate (FDR) correction, multivariate regression analysis showed a significant positive correlation between TBLH BMD, PELV BMD, and butyric acid, propionic acid, and total SCFAs (β=0.15~0.23, P<0.05).
Conclusion:
Fecal butyric acid, propionic acid, and total SCFAs are positively correlated with bone mineral density (BMD) in healthy children aged 6~9.
2024 Vol. 30 (1): 141-146 [
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32
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147
Expression of miR-181b miR-210 and miR-126 in Pregnancy-Induced Hypertensive Disorders and Their Correlation with Inflammatory Response
LIU Yang, ZHAO Qiaomian, LI Haiyan, et al
DOI: 10.3969/j.issn.1006-6233.2024.01.029
Objective:
To explore the expressions of serum micrornA-181b (miR-181b), miR-210 and miR-126 in hypertensive diseases in pregnancy (HDP) and its correlation with inflammation response.Methods:A total of 213 cases of singleton pregnant women with HDP treated at Chengde Maternal and Child Health Hospital from January 2018 to December 2019 were selected as the observation group. Based on the severity of the condition, they were divided into gestational hypertension (GH group, 62 cases), mild preeclampsia (MP group, 90 cases), and severe preeclampsia (SP group, 61 cases). Additionally, 78 healthy pregnant women treated during the same period in the same hospital were selected as the control group. The relative expression levels of miR-181b, miR-210, and miR-126, as well as serum interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-17 (IL-17), tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein-1 (MCP-1), and vascular cell adhesion molecule-1 (VCAM-1) levels were detected. The correlation between various indicators in HDP patients was analyzed.
Results:
The relative expression levels of miR-181b, miR-210, IL-1β, IL-6, IL-17, TNF-α, MCP-1, and VCAM-1 in the observation group were higher than those in the control group (P<0.05). The relative expression level of miR-126 and IL-10 in the observation group were lower than those in the control group (P<0.05). In the GH group, the relative expression levels of miR-181b, miR-210, IL-1β, IL-6, IL-17, TNF-α, MCP-1, and VCAM-1 were lower than those in the MP and SP groups, and the MP group was lower than the SP group (P<0.05). The relative expression level of miR-126 and IL-10 in the GH group were higher than those in the MP and SP groups, and the MP group was higher than the SP group (P<0.05). Pearson correlation analysis showed that the relative expression levels of miR-181b, miR-210 in HDP patients were positively correlated with IL-1β, IL-6, IL-17, TNF-α, MCP-1, and VCAM-1, and negatively correlated with IL-10 (P<0.05). The relative expression level of miR-126 in HDP patients was negatively correlated with IL-1β, IL-6, IL-17, TNF-α, MCP-1, and VCAM-1, and positively correlated with IL-10 (P<0.05).
Conclusion:
miR-181b, miR-210 are highly expressed, and miR-126 is lowly expressed in HDP. All three are closely related to the inflammatory response in HDP.
2024 Vol. 30 (1): 147-152 [
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28
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Comparison of the Effects of Two Anti-Infective Drugs on Severe Pneumonia, Inflammatory Factor Levels, and Prognosis
WANG Liangliang, et al
DOI: 10.3969/j.issn.1006-6233.2024.01.030
Objective:
To explore the effectiveness of piperacillin tazobactam and imipenem cilastatin sodium in the treatment of severe pneumonia and their impact on inflammatory factor levels and prognosis.
Methods:
A total of 105 patients with severe pneumonia admitted to our hospital from January 2020 to March 2023 were selected. Using a random number table, they were divided into Group A (n=53) treated with imipenem cilastatin sodium and Group B (n=52) treated with piperacillin tazobactam sodium. Clinical symptom disappearance times (cough, pulmonary shadow, pulmonary rales, temperature recovery time) were compared between the two groups. The severity [Clinical Pulmonary Infection Score (CPIS), Acute Physiology and Chronic Health Evaluation Ⅱ (APACHE Ⅱ), Murray Lung Injury Score (MLIS)], blood gas parameters [oxygen saturation (SpO
2
), partial pressure of oxygen (PaO
2
), partial pressure of carbon dioxide (PaCO
2
)], and levels of inflammatory factors [white blood cell count (WBC), C-reactive protein (CRP), procalcitonin (PCT)] were compared before and after 7 days of treatment. Adverse reactions were recorded.
Results:
Group B had shorter times for cough, disappearance of pulmonary shadow, pulmonary rales, and temperature recovery than Group A (P<0.05). After 7 days of treatment, both groups showed significant reductions in CPIS, APACHE Ⅱ, MLIS scores, PaCO
2
, and inflammatory factors (WBC, CRP, PCT), with larger reductions in Group B (P<0.05). Some blood gas parameters (SpO
2
, PaO
2
) significantly increased, with greater changes in Group B (P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05).
Conclusion:
Piperacillin tazobactam sodium and imipenem cilastatin sodium can alleviate the condition and clinical symptoms of patients with severe pneumonia, improve blood gas conditions, and relieve inflammatory reactions. However, piperacillin tazobactam sodium shows better efficacy.
2024 Vol. 30 (1): 153-157 [
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The Impact of Bevacizumab Combined with Capecitabine Maintenance on the Prognosis of Patients with Advanced Colorectal Cancer
HU Lixia, WANG Chao
DOI: 10.3969/j.issn.1006-6233.2024.01.031
Objective:
To explore the impact of bevacizumab combined with capecitabine maintenance on the prognosis of patients with advanced colorectal cancer.
Methods:
Clinical data of 101 patients with advanced colorectal cancer treated in our hospital from January 2017 to January 2019 were collected. All patients received first-line chemotherapy based on 5-fluorouracil and capecitabine, combined with bevacizumab and mFOLFOX6 or XELOX regimen. Among them, 42 patients received capecitabine monotherapy maintenance treatment after first-line chemotherapy (control group), and 59 patients received bevacizumab combined with capecitabine maintenance treatment (observation group). Both groups were treated until disease progression or intolerable toxic reactions. The maintenance treatment time, disease control time, Karnofsky Performance Status (KPS), Eastern Cooperative Oncology Group performance status (ZPS), serum tumor marker levels, adverse reactions, and long-term prognosis were compared between the two groups.
Results:
The maintenance treatment time and disease control time in the observation group were both longer than those in the control group (P<0.05). Both groups showed an increase in KPS and a decrease in ZPS after treatment, and the change in the observation group was greater than that in the control group (P<0.05). The serum levels of CEA, CA199, and CA724 in both groups decreased compared to before treatment, and the change in the observation group was greater than that in the control group (P<0.05). There was no statistically significant difference in the occurrence of adverse reactions between the two groups (P>0.05). The overall survival rate in the observation group was higher than that in the control group (P<0.05).
Conclusion:
Bevacizumab combined with capecitabine maintenance treatment after first-line treatment for advanced colorectal cancer is safe and effective, prolonging survival time with mild adverse reactions.
2024 Vol. 30 (1): 158-163 [
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Effect of Metadoxine Treatment on Time to Awakening Time to Symptom Disappearance and Oxidative Stress in Patients with Acute Alcohol Intoxication
WANG Xiaoyin, ZUO Shuang, XU Liyou, et al
DOI: 10.3969/j.issn.1006-6233.2024.01.032
Objective:
To explore the effects of Metadoxine treatment in patients with acute alcoholism.
Methods:
A total of 104 patients with acute alcoholism admitted to the emergency department of our hospital from January 2020 to December 2022 were selected as the study subjects, and were randomly divided into the conventional group (n=52) and the Metadoxine group (n=52) by using the method of randomized numerical table. The control group was given conventional treatment, and the observation group was given Metadoxine on the basis of conventional treatment. Clinical indexes (awakening time, symptom disappearance time) were recorded in the two groups, blood gas indexes [blood oxygen saturation (SaO
2
), partial pressure of oxygen (PO
2
)] and breath alcohol concentration (BrAC) levels were compared between the two groups before and after treatment, oxidative stress [superoxide dismutase (SOD)] status was compared between the two groups before and after treatment, and liver and kidney function [alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood creatinine (Scr), urea nitrogen (BUN)], and coagulation function [prothrombin time (PT), prothrombin time (TT), activated partial thromboplastin time (APTT), fibrinogen (FIB)].
Results:
The awakening time and symptom disappearance time of the Metadoxine group were shorter than that of the conventional group (P all <0.05); after treatment, the levels of SaO
2
, PO
2
, SOD and FIB of the two groups increased compared with those before treatment, and the level of change of Metadoxine was higher than that of the conventional group (P all <0.05); the levels of BrAC, liver and renal function indexes (ALT, AST, Scr, BUN), and some of the indicators of coagulation function (PT, TT, APTT) levels were lower than before treatment, and the level of change of Metadoxine was higher than that of the conventional group (all P<0.05).
Conclusion:
Metadoxine can shorten the awakening time and symptom disappearance time of patients with acute alcohol intoxication, improve the blood gas status of patients, alleviate the oxidative stress reaction of patients, and improve the liver and kidney function and coagulation function of patients.
2024 Vol. 30 (1): 163-168 [
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