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2023 Vol. 29, No. 9
Published: 2023-09-30
1409
Role of miR-891a-5p on Tumor Biological Behavior and Angiogenic Potential of Colon Cancer Cells
KANG Lili, CHEN Zhaowei, SONG Jian
DOI: 10.3969/j.issn.1006-6233.2023.09.01
Objective:
To explore the effects of miR-891a-5p on the proliferation, invasion, migration, apoptosis and angiogenesis of colon cancer cells and its mechanism.
Methods:
The cell with the highest expression of miR-891a-5p in each cell line of FHC, SW480, HCT116, HCT8, and SW620 was screened by PCR for SW480, and miR-891a-5p mimic (miR-891a-5p mimic), miR-891a-5p inhibitor (miR-891a-5p inhibitor) and their corresponding controls were transfected into SW480 cells. SW480 were divided into OE-NC group and OE-NLRP7, and the proliferative ability of SW480 was determined by CCK-8 method, the invasive ability of SW480 was determined by Transwell test, and the migratory ability of SW480 was determined by scratch test; the protein expression of VEGF and MMP-9 was analyzed by Western blot; and the angiogenic ability was examined by HUVEC angiogenesis assay. The targeting relationship between miR-891a-5p and NLRP7 was validated with luciferase reporter gene assay; the interaction between miR-891a-5p and NLRP7 was analyzed with RNA immunoprecipitation.
Results:
The expression of miR-891a-5p in colon cancer cells was significantly higher than that in human normal colon epithelial cells, and the expression of NLRP7 in colon cancer cells was significantly lower than that in normal stem cells. Inhibition of miR-891a-5p significantly decreased the proliferation, invasion, migration and angiogenesis of SW480 cells (P<0.01). The protein expression levels of VEGF and MMP-9 were significantly decreased (P<0.01). The results of targeting relationship showed that there was a targeting relationship between miR-891a-5p and NLRP7. NLRP7 overexpression could reduce the proliferation, migration, and angiogenesis of SW480 cells (P<0.01).
Conclusion:
miR-891a-5p can regulate NLRP7 to promote the proliferation, invasion, migration and angiogenesis of colon cancer cells to accelerate the development of colon cancer.
2023 Vol. 29 (9): 1409-1414 [
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69
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1415
Impacts of Quercetin on the Biological Behavior of Thyroid Papillary Carcinoma Cells by Regulating SOCS3/STAT3 Signaling Pathway
HAN Ke, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.02
Objective:
To investigate the impacts of quercetin (Que) on the biological behavior of thyroid papillary carcinoma (PTC) cells by regulating cytokine signal transduction inhibitor 3 (SOCS3)/signal transduction and activator of transcription 3 (STAT3) signaling pathway.
Methods:
TPC-1 cells were grouped into control group, Que low (Que L, 25 μmoL/L Que), medium (Que M, 50 μmoL/L Que), high (Que H, 100 μmoL/L Que) concentration groups, Que H+shRNA NC group (transfected with shRNA NC using Lipofectamine 2000 transfection kit, and then treated with 100 μmoL/L Que), Que H+SOCS3 shRNA group (transfected with SOCS3 shRNA using Lipofectamine 2000 transfection kit, and then treated with 100 μmoL/L Que), the apoptosis of TPC-1 cells was detected by flow cytometry; the changes in TPC-1 cell proliferation, migration, and invasion were detected by CCK-8 and Transwell experiments; the expression levels of SOCS3 and STAT3 mRNA in TPC-1 cells were detected by qRT-PCR; Western blot was applied to detect the expression levels of SOCS3, p-STAT3/STAT3, and phosphorylated non receptor tyrosine kinase Janus kinase 2 (p-JAK2)/JAK2 in TPC-1 cells.
Results:
Compared with the control group, the apoptosis rate of TPC-1 cells and the expression of SOCS3 mRNA and protein in Que-L, Que-M, and Que-H groups were obviously increased, the proliferation rate, the numbers of invasion and migration, the expression of STAT3 mRNA, p-STAT3/STAT3, and p-JAK2/JAK2 were obviously decreased (P<0.05); compared with Que-H+shRNA NC group, the apoptosis rate of TPC-1 cells and the expression of SOCS3 mRNA and protein in Que-H+SOCS3 shRNA group were obviously decreased, the proliferation rate, the numbers of invasion and migration, the expression of STAT3 mRNA, p-STAT3/STAT3, and p-JAK2/JAK2 were obviously increased (P<0.05).
Conclusion:
Que can inhibit the malignant biological behavior of PTC cell line TPC-1 by activating the SOCS3/STAT3 signaling pathway.
2023 Vol. 29 (9): 1415-1420 [
Abstract
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48
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1421
The Mechanism of miR-141-3p Targeting CDC25B to Regulate VEGF Pathway on Angiogenesis of Triple-Negative Breast Cancer
CHEN Yanzhi
DOI: 10.3969/j.issn.1006-6233.2023.09.03
Objective:
To investigate the exact function and mechanism of CDC25B in TNBC angiogenesis.
Methods:
Bioinformatics database was used to analyze the expressions of CDC25B and its upstream regulatory molecule miR-141-3p in TNBC tumor tissues, and qRT-PCR was used to analyze the expressions of CDC25B and miR-141-3p in TNBC cell lines. The proliferation and angiogenesis capacity of HUVEC cells were analyzed by CCK-8 and angiogenesis assay, and the expressions of VEGFA, VEGFR-2 and VEGFR-3 proteins were detected by Western blot. Dual luciferase reporter assay was used to explore the specific interaction between CDC25B and miR-141-3p.
Results:
It was found that CDC25B was up-regulated in TNBC, and its high expression could activate the VEGF signaling pathway, and the silencing of CDC25B significantly inhibited the proliferation and angiogenesis of HUVEC cells, and decreased the expression of VEGFA, VEGFR-2 and VEGFR-3 proteins. In addition, the expression of miR-141-3p was down-regulated in TNBC, which could target the inhibition of CDC25B expression. Overexpression of CDC25B reversed the inhibitory effect of miR-141-3p overexpression on proliferation and angiogenesis of HUVEC cells.
Conclusion:
miR-141-3p targets CDC25B to inhibit VEGF pathway and inhibit TNBC angiogenesis, and we provide theoretical basis for the possibility of miR-141-3p/CDC25B/VEGF pathway as a new choice for TNBC anti-angiogenesis therapy.
2023 Vol. 29 (9): 1421-1425 [
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44
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1426
Effects of Alteplase Combined with Safflower Extract on ACI in Rats through the Regulation of TLR4-NLRP3 Signaling Pathway
LIU Mingliang, YANG Senlin, MA Chao
DOI: 10.3969/j.issn.1006-6233.2023.09.04
Objective:
To study the ameliorative effect of intravenous thrombolysis with alteplase combined with safflower extract on acute cerebral infarction (ACI) and the potential mechanism.
Methods:
Among 30 SD rats, 6 were randomly selected as sham-operated group, and the rest were constructed as acute cerebral infarction model. After successful construction of the model, the modeled rats were randomly divided into model group, alteplase group (tail vein injection of alteplase 5 mg/kg), safflower extract group (tail vein intravenous 4 g/kg), and alteplase combined with safflower extract group, 6 rats in each group. Rats in each administration group were given the corresponding interventions, and rats in the sham-operated and model groups were injected with an equal volume of sterile 0.9% sodium chloride solution for 7 days. The neurological deficits were scored by Zea Longa; the infarct area was evaluated by TTC staining; histopathological changes were observed by HE staining; Evans blue content was determined; the apoptosis was detected by TUNEL method; TLR4, NF-κB p65, NLRP3 mRNA expression in brain tissue was detected by qRT-PCR; Western blotting analysis of protein expression of MMP-2, MMP-9, Bax, Bcl-2, TLR4, NF-κB p65, p-NF-κB p65, NLRP3, caspase1, pro-IL-1β in brain tissues.
Results:
Compared with the sham-operated group, the model group had significantly higher Zea Longa score, significantly increased infarct area, and EB content, apoptosis rate, MMP-2, MMP-9, Bax, TLR4, NF-κB p65, NLRP3, p-NF-κB p65, caspase1, pro-IL-1β protein expression and TLR4, NF-κB p65, NLRP3 mRNA expression were significantly higher and Bcl-2 protein expression was significantly lower (P<0.05). Compared with the model group, alteplase combined with safflower extract group could significantly reduce Zea Longa score and infarct area, EB content, apoptosis rate, MMP-2, MMP-9, Bax, TLR4, NF-κB p65, NLRP3, p-NF-κB p65, caspase1, pro-IL-1β protein expression and TLR4, NF-κB p65, NLRP3 mRNA expression was significantly decreased and Bcl-2 protein expression was significantly increased (P<0.05).
Conclusion:
Alteplase intravenous thrombolysis combined with safflower extract has a protective effect in rats with acute cerebral infarction, and it is a potential therapy to improve the impaired neurological function in acute cerebral infarction by inhibiting TLR4-NLRP3 signaling pathway and reducing apoptosis.
2023 Vol. 29 (9): 1426-1431 [
Abstract
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50
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1432
HIF-1 Regulates PAD4-Mediated H3cit and Promotes Apoptosis of Endometrial Glandular Epithelial Cells under Hypoxia
HUANG Yong, LUO Shu, LIAO Yuan, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.05
Objective:
To investigate the effects of HIF-1 and PAD4 on apoptosis of endometrial epithelial cells under hypoxia and its related mechanisms.
Methods:
Endometrial glandular epithelial cells CM-H058 were cultured under normal and anoxic conditions, and the expressions of HIF-1, PAD4, H3cit, Caspase3 and Caspase7 were detected by qRT-PCR and Western blot. The apoptosis level of cells was detected by flow cytometry. sh-NC, sh-HIF-1, oe-NC and oe-PAD4 were transfected into CM-H058 by lentivirus transfection method, then cultured in anoxic environment and divided into sh-NC group, sh-HIF-1 group, sh-HIF-1+oe-NC group, and sh-HIF-1+ oe-PAD4 group. The expressions of HIF-1, PAD4, H3cit, Caspase3, Caspase7 and apoptosis of cells in each group were detected. The concentration of PAD4, H3cit, and H3K27ac in TSS of Caspase3 and Caspase7 were detected.
Results:
Compared with the normal oxygen group, the expressions of HIF-1, PAD4, H3cit, Caspase3 and Caspase7 in the hypoxia group were increased (P<0.05), and the apoptosis level was increased (P<0.05). Compared with the sh-NC group, the expressions of HIF-1, PAD4, H3cit, Caspase3 and Caspase7 in sh-HIF-1 group were decreased (P<0.05), and the apoptosis level was decreased (P<0.05). Compared with sh-HIF-1+oe-NC group, the expressions of PAD4, H3cit, Caspase3, and Caspase7 in sh-HIF-1+oe-PAD4 group were increased (P<0.05), and the apoptosis level was increased (P<0.05), and the enrichment level of PAD4, H3cit and H3K27ac in TSS of Caspase3, and Caspase7 were increased (P<0.05).
Conclusion:
HIF-1 promotes PAD4 expression. PAD4 and its catalyzed H3cit further enriched Caspase3 and Caspase7 TSS, promoted the transcription and expression of Caspase3 and Caspase7, and finally induced apoptosis of endometrial epithelial cells.
2023 Vol. 29 (9): 1432-1437 [
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1438
Impact of Baicalin on Malignant Biological Behavior of Tongue Cancer Cells by Regulating Notch/JAG1 Signaling Pathway
WANG Huan, XIA Li, LI Wei, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.06
Objective:
To investigate the impact of baicalin (Bai) on the malignant biological behavior of tongue cancer cells by regulating Notch/JAG1 signal pathway.
Methods:
Human oral epithelial cells HOEC was treated with 0, 10, 20, 30, 40, 50 and 100 μM Bai. Human tongue phosphorous carcinoma cell line CAL27 was grouped into CAL27 group (untreated CAL27 cells), L-Bai group (10μM Bai treated CAL27 cells), M-Bai group (20μM Bai treated CAL27 cells), H-Bai group (40μM Bai treated CAL27 cells), VPA group (8 mM Notch/JAG1 pathway activator VPA treated CAL27 cells), and H-Bai+VPA group (40μM Bai and 8 mM VPA co-treated CAL27 cells); MTT assay was applied to detect the effects of Bai on HOEC cytotoxicity and CAL27 cell viability; the apoptosis of CAL27 cells was detected by flow cytometry; Transwell was applied to detect the invasion and migration abilities of CAL27 cells; Western blot was applied to detect the expression of epithelial-mesenchymal transformation (EMT) protein and Notch/JAG1 signaling pathway protein.
Results:
0~100μM Bai had no obvious toxic effect on HOEC cells. Compared with CAL27 group, the OD value, cell migration number, invasion number, the protein levels of vimentin, Snail, the protein levels of Notch1 and JAG1 in L-Bai group, M-Bai group and H-Bai group decreased obviously in turn (P<0.05), the apoptosis rate and the level of E-cadherin protein increased obviously in turn (P<0.05), in the VPA group, the above indicators had the opposite trends; compared with H-Bai group, the OD value, cell migration number, invasion number, the protein levels of vimentin, Snail, the protein levels of Notch1 and JAG1 in H-Bai+VPA group increased obviously (P<0.05), the apoptosis rate and the level of E-cadherin protein decreased obviously (P<0.05).
Conclusion:
Bai may affect the proliferation, apoptosis and EMT of tongue cancer cells by inhibiting Notch/JAG1 signaling pathway.
2023 Vol. 29 (9): 1438-1443 [
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32
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1444
Effect of Esculetin on Antioxidative Capacity of Rat Myocardial Tissue
YU Xiaolei, GUO Yan, DONG Yi, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.07
Objective:
To investigate the effect of esculetin (ESC) on the oxidative stress induced by doxorubicin(DOX) in cardiomyocytes.
Methods:
SD rats were divided into control group, DOX group, ESC low dose and high dose + DOX group. The serum myocardial enzymes and SOD, MDA levels in myocardial tissue were detected in each group. The changes of collagen fibers in myocardial tissue were compared by Masson staining. The expression of related proteins in each group was detected by Western blot.
Results:
ESC can increase the SOD level and decrease the increase of myocardial enzyme and MDA caused by DOX (P<0.05); after DOX intervention, the collagen fiber in myocardial tissue increased significantly, while the collagen fiber in myocardial tissue pre-treated by ESC decreased significantly; ESC can reduce the increase of p53 content caused by DOX (P<0.05), and up-regulate the antioxidant enzyme HO-1 (P<0.05), MDM2 (P<0.05) and Nrf2(P<0.05).
Conclusion:
Ganoderma polysaccharide can improve the oxidative stress injury of myocardial tissue induced by doxorubicin and play a protective role in myocardial tissue.
2023 Vol. 29 (9): 1444-1446 [
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29
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1447
Diagnostic Value of Serum SCC M-CSF and Chemerin Levels in Patients with Cervical Intraepithelial Neoplasia
SUN Li, SONG Yurong, HU Pin, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.08
Objective:
To investigate the changes of serum squamous cell carcinoma antigen (SCC), macrophage colony stimulating factor (M-CSF), and chemokine (Chemrin) in patients with cervical epithelial neoplasia and their diagnostic value.
Methods:
A total of 101 patients with cervical epithelial neoplasia (CIN) confirmed by pathological examination in our hospital from April 2019 to April 2022 were selected as the CIN group, and 50 patients with early cervical cancer confirmed by pathological examination in the same period were selected as the cervical cancer group. The measured values of serum SCC, M-CSF, and Chemerin before treatment were compared between the two groups, and the hierarchical comparative analysis was carried out according to the CIN stage and the results of high-risk human papillomavirus (HR-HPV) viral load test. The levels of serum SCC, M-CSF and Chemerin in CIN patients before and 3 months after operation were compared. The application value of SCC, M-CSF and Chemerin in the differential diagnosis of CIN and early cervical cancer was analyzed by the subject working characteristic curve (ROC).
Results:
The measured values of serum SCC, M-CSF and Chemerin in patients with cervical cancer were significantly higher than those in patients with CIN (P<0.05). The areas under ROC curve of SCC, M-CSF and Chemerin were 0.906, 0.846 and 0.791, respectively. Among the 101 patients with CIN, there were 21 patients with stage I, 46 patients with stage II, and 34 patients with stage III. With the increase of CIN stage, the levels of SCC, M-CSF, and Chemerin in the patients increased gradually, and the difference between the groups was statistically significant (P<0.05). The measured values of SCC and M-CSF in patients with HR-HPV DNA positive CIN were higher than those in patients with negative CIN, and the difference between the groups was statistically significant (P<0.05). Three months after the operation, the measured values of SCC, M-CSF and Chemerin in CIN patients showed a significant downward trend, and the difference was statistically significant (P<0.05).
Conclusion:
Compared with cervical cancer patients, the determination values of SCC, M-CSF and Chemerin in CIN patients were lower, but with the increase of CIN grading and the higher levels of SCC, M-CSF and Chemerin in patients with positive expression of HR-HPV DNA, Detection of SCC, M-CSF and Chemerin levels can differentiate CIN from cervical cancer.
2023 Vol. 29 (9): 1447-1451 [
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26
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1452
Expression and Clinical Significance of Serum TSH TgAb and MK in Patients with BRAF Gene Mutant Thyroid Cancer
LIU Peihong, AN Liyun, SHAO Rubin, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.09
Objective:
To investigate the levels of serum thyrotropin (TSH), thyroglobulin antibody (TgAb) and intermediate factor (MK) in patients with thyroid cancer (TC) with the B1 (BRAF) gene homology of anti-mouse sarcoma virus, and to analyze the diagnostic value of TSH in patients with TC.
Methods:
83 cases BRAF gene mutant TC patients admitted to our hospital from January 2021 to January 2022 were selected as the mutation group, and 83 cases BRAF gene wild-type TC patients in the same period were selected as the wild group following the principle of 1∶1 allocation. Demographic data, clinicopathological features, serum TSH, TgAb and MK levels of the two groups were analyzed. The influencing factors of mutant TC were analyzed by Logistic regression equation. The diagnostic value of each index on mutant TC was evaluated and its influence on the risk of mutant TC were analyzed.
Results:
There were significant differences in tumor stage, capsule invasion, tumor number, cervical lymph node metastasis, central lymph node metastasis and serum TSH, TgAb and MK levels between the two groups (P<0.05). Serum TSH, TgAb and MK levels were positively correlated with BRAF gene mutation (P<0.05). Increased serum TSH, TgAb and MK levels were the risk factors for BRAF mutant TC (P<0.05). The AUC of serum TSH, TgAb and MK in the diagnosis of BRAF mutant TC was greater than that of single diagnosis (P<0.05). The risk of BRAF gene mutation TC in high TSH, TgAb and MK expression group was 1.500, 1.625 and 2.617 times higher than that in low expression group (P<0.05).
Conclusion:
The serum levels of TSH, TgAb and MK in patients with BRAF mutant TC are elevated. The combined detection of TSH, TGAb and MK levels is of certain diagnostic value for BRAF mutant TC.
2023 Vol. 29 (9): 1452-1458 [
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50
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1459
Predictive Value of HMGB1 IL-17 and Exhaled Nitric Oxide Concentrations in Peripheral Blood of Patients with COPD Combined with Respiratory Failure Type II
BO Yuan, LI Li, HE Jin, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.010
Objective:
To investigate the changes of high mobility group protein B1 (HMGB1), interleukin-17 (IL-17) and exhaled nitric oxide (FeNO) levels in peripheral blood of patients with chronic obstructive pulmonary disease (COPD) combined with respiratory failure type II, and to analyze its predictive value for prognosis.
Methods:
A total of 100 patients with COPD combined with respiratory failure type II in our hospital from January 2021 to December 2022 were selected as the observation group, 60 patients with COPD but without respiratory failure during the same period were selected as the control group, and 60 patients with healthy physical examination were selected as the healthy group. Peripheral blood HMGB1, IL-17 and FeNO were collected upon admission. Patients were classified as dead or alive based on survival at 28 days of hospitalization, and peripheral blood was analyzed for HMGB1, IL-17, and FeNO. The clinical influencing factors of prognosis were analyzed by Lasso-Logistic regression. To analyze the effect of FeNO interaction with peripheral blood HMGB1 and IL-17 on the prognosis of patients and its predictive value for prognosis.
Results:
Peripheral blood HMGB1, IL-17 and FeNO were higher in the observation group than in the control group and the healthy group, and the control group was higher than the healthy group (P<0.05); there was a positive correlation between peripheral blood HMGB1, IL-17 and FeNO (P<0.05); the proportion of acute exacerbations ≥2, the APACHE II score, peripheral blood HMGB1, IL-17 and FeNO were independent prognostic risk factors (P<0.05); there was an interaction between high expression of HMGB1, IL-17, and FeNO and poor prognosis (P<0.05); and the AUC of the combination of HMGB1, IL-17, and FeNO in predicting prognosis was higher than that predicted by the index alone (P<0.05).
Conclusion:
The peripheral blood concentrations of HMGB1, IL-17 and FeNO were elevated in patients with COPD combined with type II respiratory failure and had synergistic effects in poor prognosis, and the combined detection of their levels to predict prognosis has clinical application value.
2023 Vol. 29 (9): 1459-1464 [
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34
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1465
The Diagnostic and Prognostic Value of miR-2116-3p and miR-342-3p in Hepatocellular Carcinoma
BAI Ziyu, LI Erqi, LIU Hui, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.011
Objective:
To investigate the clinical value of miR-342-3p and miR-2116-3p in hepatocellular carcinoma (HCC).
Methods:
A total of 57 samples of tumor tissues and para-tumor liver tissues from HCC patients who underwent curative resection were included in this study. The expression levels of miR-2116-3p and miR-342-3p were measured and analyzed by qRT-PCR.
Results:
The expression levels of miR-2116-3p and miR-342-3p in HCC tissues were significantly lower than those in adjacent tissues (P=0.034, P<0.001). ROC curve analysis showed that the two had diagnostic efficacy (P<0.05). The expression of miR-2116-3p can be used as an independent risk factor for predicting the poor prognosis of HCC patients.
Conclusion:
In the 57 samples of this study, miR-2116-3p as well as miR-342-3p were significantly reduced in hepatitis-associated HCC tissues, which had diagnostic value for HCC as a potential new marker; miR-2116-3p was an independent predictor of poor prognosis in HCC.
2023 Vol. 29 (9): 1465-1472 [
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35
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1473
Effect of Ultrasound-Guided Stellate Ganglion in Patients Undergoing Thoracoscopic Surgery for Lung Cancer and Risk Factors of Postoperative Acute Pain
WANG Weili, LIU Jiandong HUANG Mingwei, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.012
Objective:
To investigate the effect of ultrasound-guided stellate ganglion block (SGB) in patients with thoracoscopy for lung cancer and analyze the risk factors for acute postoperative pain.
Methods:
A total of 98 patients undergoing thoracoscopic surgery for lung cancer in our hospital from January 2021 to December 2022 were randomly divided into 2 groups, 49 cases in the control group and 49 cases in the study group. In the study group, a right stellate ganglion block was performed at the C6~7 level under ultrasound guidance, and 5mL of 0.5% ropivacaine was given, while in the control group, 5 mL of saline was given at the same location to compare the two groups with perioperative indicators before stellate ganglion block (T
1
), 5 min (T
2
) after extubation, and 6:00 am (T
3
). After one day, pain was assessed by visual analogue scoring method (VAS). According to the VAS score, 29 patients were divided into the acute pain group (VAS 3 points) and 69 patients in the non-acute pain group (VAS <3 points). The risk factors for postoperative acute pain were analyzed.
Results:
Postoperative extubation time, time to leave the recovery room to SM, sufentanil use, PAPT, postoperative delirium rate, nausea and vomiting rate of the study group were not statistically significant compared with that of the control group (P>0.05), and the postoperative discharge time of the study group was significantly shorter than that of the control group (P<0.05).The levels of epinephrine (E) and norepinephrine (NE) were elevated in both groups at the T
2
and T
3
time points compared with the T
1
time point (P<0.05), but E and NE were significantly lower in the study group than in the control group (P<0.05). After univariate analysis, age, operation time, intercostal space width, and psychological anxiety were the influencing factors of postoperative acute pain in thoracoscopic patients with lung cancer (P<0.05), and after multifactorial binary logistic regression analysis, operation time ≥150min and psychological anxiety were the risk factors of postoperative acute pain in thoracoscopic patients with lung cancer, and intercostal space width ≥1cm was a postoperative protective factor for acute pain (P<0.05).
Conclusion:
Ultrasound-guided SGB applied to patients undergoing thoracoscopic surgery for lung cancer effectively reduces stress and shortens patients' hospitalization time; postoperative pain is associated with operative time ≥150 min, intercostal space width <1 cm, and psychological anxiety.
2023 Vol. 29 (9): 1473-1478 [
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1479
Clinical Characteristics and Clinical Significance of Changes in NT-proBNP and Echocardiographic Parameters in Elderly with Ejection Fraction Preserved Heart Failure Combined with Debility
LV Qiu, QIU Qingyong, DU Lanxue, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.013
Objective:
To analyze characteristics in elderly patients with heart failure with preserved ejection fraction (HFpEF) and frailty, and to explore the clinical significance of peripheral blood N-terminal pro-B-type natriuretic peptide (NT-proBNP) and echocardiogram parameters.
Methods:
A total of 96 patients with HFpEF admitted to the hospital were enrolled between February 2021 and February 2023. The frailty was evaluated by Fried Frailty Evaluation Criteria within 24h after admission. According to the evaluation results, patients were divided into frailty group (n=56) and non-frailty group (n=40). The clinical data in the two groups were collected. The risk factors of frailty were analyzed by univariate analysis and multivariate Logsitic regression analysis.
Results:
Compared with non-frailty group, the age, proportion of NYHA grading at grade III/IV, proportion of nutritional risk and NT-proBNP level in frailty group were higher (P<0.05), while levels of hemoglobin (Hb) and albumin (ALB) were lower (P<0.05). The left ventricular ejection fraction (LVEF) in frailty group was lower (P<0.05), while left ventricular end-diastolic diameter (LVDD), left ventricular end-systolic diameter (LVDS) aortic diameter (AOD) were longer or larger (P<0.05). There was no significant difference in left atrial diameter (LVDS) or left ventricular posterior wall thickness (LVPW) between the two groups (P>0.05). Multivariate Logistic regression showed advanced age, NYHA grading at grade III/IV, high-level NT-proBNP and widened AOD were independent risk factors of frailty in elderly patients with HFpEF (P<0.05).
Conclusion:
Elderly patients with HFpEF have high incidence of frailty. Advanced age, NYHA grading at grade III/IV, high-level NT-proBNP, and widened AOD are independent risk factors of frailty.
2023 Vol. 29 (9): 1479-1483 [
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46
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1484
The Value of Serum Caspase-3 NSE and O
2
UCc in Early Prediction of Delayed Encephalopathy Caused by Acute Carbon Monoxide Poisoning
BAI Yanyan, LI Ruibo, ZHAO Rui, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.014
Objective:
To investigate the value of cysteine aspartic protease-3 (caspase-3), neuron-specific enolase (NSE) and early cerebral oxygen utilization (O
2
UCc) in early prediction of delayed encephalopathy caused by acute carbon monoxide poisoning.
Methods:
A total of 125 patients with acute carbon monoxide poisoning admitted to our hospital from January 2019 to December 2020 were selected. According to whether delayed encephalopathy of carbon monoxide poisoning occurred within 60 days after poisoning, the patients were divided into encephalopathy group (34 cases) and control group (91 cases). Serum caspase-3, NSE and O
2
UCc levels of the two groups were compared within 24h after admission, and the value of each index in predicting delayed encephalopathy in patients with acute carbon monoxide poisoning was analyzed by receiver operating curve (ROC). Logistic multivariate model was used to analyze the relationship between caspase-3, NSE, O
2
UCc and delayed encephalopathy caused by carbon monoxide poisoning.
Results:
The measured values of caspase-3 and NSE in encephalopathy group were significantly higher than those in control group, while the measured values of O
2
UCc in encephalopathy group were significantly lower than those in control group, with statistical significance (P<0.05). The sensitivity of caspase-3, NSE, O
2
UCc, caspase-3+NSE+O
2
UCc to predict delayed encephalopathy of acute carbon monoxide poisoning were 63.41%, 57.52%, 62.77% and 92.33%, respectively. The specificity was 85.28%, 80.66%, 82.94%, 78.58%, and the area AUC under ROC curve was 0.802, 0.797, 0.798, 0.936, respectively. Logistic regression model showed that aging, prolonged CO exposure time, consciousness disturbance ≥10h, severe poisoning, increased caspase-3, increased NSE and decreased O
2
UCc were independent risk factors for delayed encephalopathy of acute carbon monoxide poisoning (P<0.05).
Conclusion:
Early caspase-3 and NSE levels were significantly increased and O
2
UCc levels were significantly decreased in patients with acute carbon monoxide poisoning delayed encephalopathy, and there was a significant relationship with the occurrence of delayed encephalopathy in patients with acute carbon monoxide poisoning.
2023 Vol. 29 (9): 1484-1488 [
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33
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1489
Effects of Minimally Invasive Surround-Mammary Areola Incision Surgery and Open Resection Surgery in the Treatment of Gynecomastia
HE Jiao, ZHOU Yuhui, MA Xiaoxia, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.015
Objective:
To explore the effects of minimally invasive surround-mammary areola incision surgery and open resection surgery in the treatment of gynecomastia (GYN).
Methods:
A total of 105 patients with GYN admitted to the hospital from August 2020 to August 2022 were selected and divided into two groups according to different surgical treatment regimens. Among the patients, 63 cases with GYN undergoing minimally invasive surround-mammary areola incision surgery were included in observation group, and 42 cases receiving open resection surgery were enrolled as control group. The surgery-related indicators, wound healing time, scar width, breast beauty, preoperative and postoperative traumatic stress indicators [norepinephrine (NE), epinephrine (E), cortisol (Cor)], occurrence of postoperative complications and postoperative effect satisfaction were compared between the two groups.
Results:
The surgical time, hospital stay and wound healing time in observation group were shorter than those in control group (P<0.05), and the intraoperative blood loss was less than that in control group (P<0.05), and the incision length and scar length were shorter compared with those in control group (P<0.05), and the breast beauty was higher compared to control group (P<0.05). The levels of serum NE, E and Cor in both groups were higher after surgery than those before surgery (P<0.05), but the decreases in observation group were lower than those in control group (P<0.05). The total incidence rate of postoperative complications was lower in observation group than that in control group (3.17% vs 21.43%, P<0.05). The satisfaction scores of postoperative chest appearance, wound scar and skin and areola nipple sensation and overall satisfaction score in observation group were higher compared with those in control group (P<0.05).
Conclusion:
Compared with open resection surgery, minimally invasive surround-mammary areola incision surgery for patients with GYN has shorter surgical time, wound healing time and hospital stay, less intraoperative blood loss and shorter postoperative incision length and scar length, and the latter one can better significantly relieve the postoperative traumatic stress response, reduce the postoperative complications, and enhance the patients’ satisfaction with postoperative effect.
2023 Vol. 29 (9): 1489-1493 [
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41
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1494
Correlation Analysis between Preoperative Endoscopic Score and Poor Prognosis of Patients with Esophageal Cancer after Endoscopic Submucosal Dissection
ZHOU Li, GUO Lan, ZOU Xuemei, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.016
Objective:
To investigate the characteristics of changes in lipoprotein-related phospholipase A2 after intravenous thrombolysis with alteplase in patients with acute cerebral infarction and the effect on cognitive function recovery.
Methods:
One hundred and twenty-eight patients with acute cerebral infarction admitted to our hospital for intravenous thrombolysis with alteplase from January 2022 to February 2023 were selected. According to the evaluation results of the postoperative MoCA scale, the patients with MoCA score <26 were included in the poor recovery group (18 cases), and those with MoCA score ≥26 were included in the good recovery group (110 cases). The poor recovery group was randomly divided into a training set and a verification set. The change trend and clinical characteristics of Lp-PLA2 before and after treatment were compared between the two groups, and the relationship between the variables and outcome events (adverse recovery of cognitive function) was determined by multivariate Logistic regression analysis, and the prediction line graph and evaluation were drawn based on the risk factors. Then the model was stratified according to the risk score of the column graph by X-tile software, and the clinical application value of the model was further discussed.
Results:
The Lp-PLA2 levels in both groups were decreased after intravenous thrombolytic therapy with alteplase, but the decrease in the good recovery group (223.45±102.35 μg/L) was higher than that in the poor recovery group (289.36±110.23 μg/L), and the difference between the two groups was statistically significant (P<0.05). Age ≥65 years old, infarct site (frontal lobe), pre-thrombolysis hyperglycemia, fibrinogen, white blood cell count, Lp-PLA2, metabolic syndrome, APTT, and thrombolysis time in poor recovery group were all higher than those in good recovery group, while NIHSS score and MESSS score after treatment were lower than those in good recovery group (P<0.05). Infarct site (frontal cortex), pre-thrombolysis hyperglycemia, fibrinogen ≥2.89g/L, white blood cell count ≥8.00×10
9
/L, Lp-PLA2≥291.36ng/mL, metabolic syndrome, APTT≥31.50s, thrombolysis time ≥4.50s, NIHSS score ≥15.00, and MESSS score ≥12.00 were the risk factor for poor cognitive recovery after intravenous thrombolysis of alteplase (P<0.05). The results of risk prediction model line graph evaluation showed that the C-index of training set and verification set were 0.862 and 0.826, respectively. The calibration curves of the two sets fit well with the ideal curves. The area under ROC curve (AUC) of the training and validation sets were 0.864 (95%CI: 0.789~0.898, P<0.001) and 0.834 (95%CI: 0.774~0.877, P<0.001), respectively. The decision curve showed that when the threshold probability was 0.01 ~ 0.91, the net benefit rate was > 0, which had a high net benefit value, safe and reliable, with strong practicability. The incidence of poor recovery in high risk group was significantly higher than that in medium risk group and low risk group (χ
2
=6.266, P=0.013).
Conclusion:
Based on this ideal and practical predictive model, we can identify and actively intervene early in patients with acute cerebral infarction after intravenous thrombolysis to improve their prognosis.
2023 Vol. 29 (9): 1494-1501 [
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27
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1502
Comparison on the Clinical Curative Effect of Single-Port Thoracoscopic Precise Segmentectomy and Lobectomy on Early Non-Small Cell Lung Cancer
HU Guoliang, REN Dan, TU Qimin, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.017
Objective:
To compare and analyze the clinical curative effect of two single-port thoracoscopy on early non-small cell lung cancer (NSCLC).
Methods:
A retrospective analysis was performed on the clinical data of 108 patients with early NSCLC admitted to the hospital between January 2020 and October 2022. According to different surgical methods, they were divided into single-port thoracoscopic precise segmentectomy group (group A, 58 cases) and lobectomy group (group B, 50 cases). All were followed up for 5 months after surgery. The differences in perioperative indexes (total number of lymph nodes dissection, intraoperative blood loss, operation time, indwelling time of thoracic catheter, thoracic drainage volume, hospitalization time), tumor markers [cytokeratin-19-fragment (CYFRA21-1), carcinoembryonic antigen (CEA), carbohydrate antigen 50 (CA50)], lung function indexes [peak expiratory flow (PEF), forced vital capacity (FVC), forced expiratory volume in one second (FEV1)], differences in the Quality of Survival in Lung Cancer Scale (EORTC QLQ-LC43) scores, and postoperative complication rates were compared.
Results:
The differences in the total number of lymph node dissection, intraoperative blood loss, and complication rates between the two groups were not statistically significant (P>0.05), and the operative time in Group A was higher than that in Group B. The time of chest tube retention, chest drainage, and hospitalization were lower than that in Group B (P<0.05); the levels of CYFRA21-1, CEA, and CA50 in both groups were lower than those in the preoperative period at 3 d postoperatively (P<0.05), whereas the difference between the two groups before and after surgery was not statistically significant in the same group (P>0.05); at 5 months postoperatively, the levels of PEF, FVC, and FEV1 were lower than those in the preoperative period in both groups, whereas the decrease in group A was smaller than that in group B, with a smaller difference between the pre- and post-surgical periods (P<0.05); after 5 postoperative months, all EORTC QLQ-LC43 scores in group A were higher than those in group B (P<0.05).
Conclusion:
The single-port thoracoscopic lobectomy and segmentectomy have comparable curative effect and safety. However, the latter is more beneficial to shorten the rehabilitation process, improve lung function and quality of life.
2023 Vol. 29 (9): 1502-1506 [
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38
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1507
Value of Shear Wave Elastography in Ultrasound-Guided Fine Needle Aspiration of Papillary Thyroid Carcinoma
LIU Dandan, ZHANG Huiping, ZHANG Fan, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.018
Objective:
To investigate the application value of shear wave elastography (SWE) in ultrasound-guided fine needle aspiration (FNA) of papillary thyroid carcinoma.
Methods:
The relevant data of 90 patients suspected of papillary thyroid carcinoma who were admitted to the hospital from January 2019 to December 2021 were retrospectively analyzed. All patients needed to undergo ultrasound-guided FNA. According to the ultrasound technique used, the patients were divided into conventional group (n=42, two-dimensional ultrasonography) and SWE group (n=48). The sampling results, the incidence of puncture complications, the pathological classification of Bethesda system, and the value in diagnosing benign and malignant lesions were compared between the two methods.
Results:
The success rate of sampling and the detection rate of 2 needles in the SWE group were higher than those in the conventional group, but only the detection rate of 2 needles showed a statistical significance (P<0.05). The incidence of puncture complications in the SWE group was significantly lower than that in the routine group (4.16% vs 21.42%, P<0.05). The pathological results of Bethesda classification system showed no statistically significant difference between the two groups, but the diagnosis rate of class II～VI in the SWE group was higher than that in the conventional group (P<0.05). With pathological results as the golden standard, the diagnostic sensitivity and accuracy in the SWE group for malignant lesions were higher than those in the conventional group (P<0.05).
Conclusion:
SWE is helpful for determining the location of FNA in patients suspected of papillary thyroid carcinoma, improving the detection rate of 2 needles, reducing puncture complications, and improving the diagnosis of papillary thyroid carcinoma to a certain extent.
2023 Vol. 29 (9): 1507-1511 [
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30
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1512
Effect of Robot-Assisted Tension Screw Internal Fixation Surgery on Majeed Score and Gait Parameters in Patients with Pelvic Fractures
LUO Hong
DOI: 10.3969/j.issn.1006-6233.2023.09.019
Objective:
To investigate the effect of robot-assisted tension screw internal fixation surgery on Majeed score and gait parameters in patients with pelvic fracture (PF).
Methods:
Eighty-six patients with PF in our hospital from Februarry 2022 to Februarry 2023 were selected and divided into two groups based on the treatment plan, 43 patients each. The control group underwent manual incisional repositioning and fixation, and the observation group underwent robot-assisted tension screw internal fixation. The surgical indicators (surgical time, intraoperative bleeding, time to first weight-bearing, length of hospital stay) of the two groups were compared, as well as Majeed score, Matta score, and pain status. Enzyme-linked immunosorbent assay (ELISA) was used to detect the serum stress response indicators Norepinephrine (NE), tumor necrosis factor-α (TNF-α), angiotensin Ⅱ (Ang Ⅱ), C-reactive protein (CRP) before and after surgery. Fully automated chemiluminescence immunoassay analyzer was used to analyze serum fracture healing-related indexes alkaline phosphatase (ALP), osteocalcin (BGP), osteoprotegerin (OPG), postoperative gait parameters (stride speed, stride length, and percentage of support time), and adverse effects.
Results:
The operating time, intraoperative bleeding, time to first weight-bearing on the ground and hospital stay were less in the observation group than in the control group (P<0.05); the excellent rates of Majeed score and Matta score were higher in the observation group than in the control group at 3 months after surgery (P<0.05); the degree of postoperative pain was lower in the observation group than in the control group (P<0.05). The serum NE, TNF-α, Ang II and CRP levels in the observation group were lower than those in the control group at 24 h and 72 h after surgery, and the serum ALP, BGP and OPG levels were higher than those in the control group at 1 and 3 months after surgery (P<0.05); the gait speed, stride length and percentage of support time in the observation group were higher than those in the control group at 3 and 6 months after surgery (P<0.05); the incidence of adverse reactions in the observation group was lower than that in the control group (P<0.05).
Conclusion:
Robot-assisted tension screw internal fixation surgery for PF can optimize the surgery, reduce the stress response and relieve the patient's pain, while promoting fracture healing, improving the patient's pelvic function, restoring the body's coordination, and improving the safety of the surgery.
2023 Vol. 29 (9): 1512-1519 [
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27
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1520
Efficacy of Ilioinguinal Approach Modified Stoppa Approach and Parabdominis Rectus Approach in Treating Pelvic and Acetabular Fractures
NI Bowen, HAO Peng, CHEN Kang
DOI: 10.3969/j.issn.1006-6233.2023.09.020
Objective:
To explore the efficacy of ilioinguinal approach, modified Stoppa approach and parabdominis rectus approach in the treatment of pelvic and acetabular fractures.
Methods:
The data of patients with pelvic and acetabular fractures in the hospital from August 2020 to August 2022 were retrospectively analyzed, and the patients were classified into three groups according to the different regimens. Patients with ilioinguinal approach were selected as group A, patients with modified Stoppa approach were included in group B, and patients with parabdominis rectus approach were enrolled as group C. According to the propensity matching scoring method, 50 cases in group A, 50 cases in group B, and 52 cases in group C were finally obtained. There were no statistical differences among the three groups in terms of baseline data (P>0.05). The general surgery, postoperative hospital stay, quality of fracture reduction at 7 days after surgery, hip function (modified Merle D’ Aubignéand Postel scoring system) at different time points (before surgery, at discharge and at the last follow-up at 6 months after surgery) and occurrence of postoperative complications were compared among the three groups.
Results:
There were statistically significant differences in fracture end exposure time, intraoperative blood loss and postoperative Hb among the three groups (P<0.05). The exposure time in groups B and C was obviously shorter compared with that in group A (P<0.05), and the intraoperative blood loss was significantly less (P<0.05), and the postoperative Hb was significantly higher (P<0.05), but there were no statistical differences in the above surgical indicators between group B and group C (P>0.05). There was no statistically significant difference in the excellent and good rate of fracture reduction quality among the three groups at 7 days after surgery (P>0.05). Although the excellent and good rate in group C was higher than that in group A and group B, there was no statistical difference between the two groups (P>0.05). At discharge and at the last follow-up at 6 months after surgery, the Postel score of the three groups was gradually improved, and there was a statistical significance from the aspect of time-point effect among the three groups (P<0.05), but there was no statistical difference in the score from the aspects of between-group effect and interaction effect of between-group and time-point (P>0.05). The Postel score in the three groups at discharge and at the last follow-up at 6 months after surgery was higher than that before surgery (P<0.05). The total postoperative complication rate was significantly different in the three groups (P<0.05), and the rate in group C was significantly lower than that in group A (9.62% vs 36.00%, P<0.05).
Conclusion:
All three kinds of plate internal fixation in the treatment of pelvic and acetabular fractures can achieve good fracture reduction quality and restore hip function, but modified Stoppa approach and parabdominis rectus approach have better effects on exposure time, blood loss and postoperative Hb than ilioinguinal approach, and parabdominis rectus approach has a higher excellent and good rate of fracture reduction than the other two approaches and fewer complications.
2023 Vol. 29 (9): 1520-1526 [
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28
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1527
Application Value of Low-Dose CT in the Diagnosis and Monitoring of Pulmonary Tuberculosis
WANG Li, GAO Yu, WANG Zhiyong, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.021
Objective:
To investigate the application value of low-dose CT in the diagnosis and monitoring of pulmonary tuberculosis.
Methods:
The clinical data of 357 patients who were suspected of pulmonary tuberculosis and treated in the hospital from March 2019 to March 2021 were analyzed retrospectively. All patients underwent conventional-dose and low-dose scanning. The diagnostic performance, detection rates of imaging features, image quality, and radiation parameters were compared between the two scanning methods.
Results:
Pathological results showed 350 patients with pulmonary tuberculosis, and 342 cases were diagnosed with low-dose scanning. The sensitivity, specificity and accuracy were 99.42%, 33.33% and 96.64%. 344 cases were diagnosed with conventional-dose scanning. The sensitivity, specificity and accuracy were 99.71%, 46.15% and 97.76%. The diagnostic sensitivity, specificity, and accuracy of the two scanning methods were similar (P>0.05). The image quality scores of cross-sectional and coronal lung window and mediastinal window were close in terms of different scanning methods (P>0.05). The detection rates of imaging signs such as lung consolidation, ground-glass opacity and calcification by the two scanning methods were close (P>0.05). The dose-length product, volume CT dose index and effective dose of low-dose scanning were lower than those of conventional-dose scanning (P<0.05).
Conclusion:
Compared with conventional-dose CT scanning, low-dose CT scanning can effectively reduce radiation dose while achieving comparable diagnostic results and image quality. It can be used for the diagnosis and monitoring of pulmonary tuberculosis.
2023 Vol. 29 (9): 1527-1531 [
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41
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1532
Analysis of Influencing Factors of Rehabilitation Training Effect and Construction of Nomogram Prediction Model in Elderly Post-Stroke Hemiplegia Patients
FENG Bing, YANG Peiquan, ZHOU Hui
DOI: 10.3969/j.issn.1006-6233.2023.09.022
Objective:
To analyze the influencing factors of rehabilitation training effect in elderly post-stroke hemiplegia patients, and to construct a nomogram model to predict the effect of rehabilitation training.
Methods:
The clinical data of 286 elderly patients with hemiplegia after acute stroke who received rehabilitation training in Guiping People's Hospital from July 2017 to June 2021 were retrospectively collected and randomly divided into modeling group (n=200) and validation group (n=86) with the ratio of 7∶3. The data of the modeling group were used to construct a risk prediction nomogram model, and the data of the validation group were used to evaluate the performance of the model. The two groups were divided into a good effect group and a poor effect group according to the effect of rehabilitation training. Univariate and multivariate Logistic regression analyses were used to identify the risk factors for poor rehabilitation training effect, R3.6.3 software was applied to build a nomogram model for predicting poor rehabilitation effect, and the effect of the nomogram model in predicting poor rehabilitation effect was verified.
Results:
Among the 286 hemiplegic patients, 42.66% (122/286) had poor rehabilitation effect. The age, male proportion, alcohol consumption proportion, hypertension proportion, rehabilitation training start time >2 weeks, and rural living environment proportion of patients in the poor effect group were significantly higher than those in the good effect group, with significant differences (P<0.05). Through multivariate Logistic regression model, it was found that living environment, age, hypertension, rehabilitation training start time >2 weeks, and alcohol consumption were the risk factors for poor rehabilitation effect of hemiplegic patients (P<0.05). The area under the ROC of the modeling group was 0.801, and the area under the curve of the validation group was 0.851; the slopes of the calibration curves of both the modeling group and the validation group were close to 1.
Conclusion:
Based on age, drinking, high blood pressure, rehabilitation training start time, and living environment, the nomogram model for predicting poor rehabilitation training effect in elderly post-stroke hemiplegia patients can better predict poor rehabilitation training effect individually, and it has certain guiding significance for early intervention in rehabilitation training.
2023 Vol. 29 (9): 1532-1537 [
Abstract
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59
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1538
Effect of Arthroscopic Double Pulley Combined with Double Row Suture Bridge Technique on Rotator Cuff Tear and Its Influence on Shoulder Function Recovery
ZHANG Xiaoyu, MA Jingzu, YU Jia, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.023
Objective:
To explore the effect of combined technique of arthroscopic double pulley and double row suture bridge in the treatment of rotator cuff tear (RCT) and its influence on shoulder function recovery.
Methods:
The clinical data of 52 patients with RCT who received traditional suture bridge technique in the hospital from July 2018 to June 2022 were retrospectively analyzed and the patients were included in control group. The clinical data of 53 patients with RCT who received arthroscopic double pulley combined with double row suture bridge technique in the hospital during the same period were retrospectively analyzed and the patients were enrolled as observation group. The surgical indicators, therapeutic effects [healing rate, re-tear rate, Visual Analogue Scale (VAS)] after 6 months of follow-up, the joint range of motion (forward flexion and upward lift, lateral external rotation, lateral internal rotation) and shoulder function [American Shoulder and Elbow Surgeons (ASES), Constant-Murley Score (CMS), University of California at Los Angeles (UCLA)] before surgery and at 6 months after surgery were compared between the two groups, and the postoperative complications were counted.
Results:
There were no significant differences in surgical time, surgical blood loss, hospital stay, and pain relief time between both groups (all P>0.05). At 6 months after surgery, VAS score in both groups was reduced compared to before the surgery, and the score in observation group was lower than that in control group (P<0.05). Forward flexion and upward lift, lateral external rotation, lateral internal rotation, ASES score, UCLA score and CMS score were enhanced compared with those before surgery, and the above indicators were higher in observation group than those in control group (all P<0.05).
Conclusion:
Arthroscopic double pulley combined with double row suture bridge technique has a significant effect in the treatment of RCT, and it can enhance the joint flexibility of patients with RCT and is beneficial to shoulder function recovery in patients with RCT.
2023 Vol. 29 (9): 1538-1542 [
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76
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1543
Efficacy of Modified Uvulopalatopharyngoplasty in the Treatment of OSAHS Patients with Cognitive Impairment and Its Effect on Respiratory Function, Electroencephalogram and MMSE Score
ZHU Yubo, CHENG Leilei, ZHU Rongfei, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.024
Objective:
To observe the curative effect of modified uvulopalatopharyngoplasty (H-UPPP) in the treatment of patients with obstructive sleep apnea hypopnea syndrome (OSAHS) with cognitive impairment and the effects on respiratory function, electroencephalogram and Montreal cognitive assessment scale ( MMSE) score.
Methods:
A total of 124 patients with moderate and severe OSAHS and cognitive impairment who were admitted to the Department of Otolaryngology, Head and Neck Surgery of Ya'an People's Hospital from February 2020 to February 2022 were selected as the research subjects. They were divided into two groups by simple random method, 62 patients in each group. The conservative group was treated with behavioral therapy intervention, and the observation group was treated with H-UPPP combined with behavioral therapy intervention. The differences of polysomnography parameters, Montreal Cognitive Assessment Scale (MOCA) score, Sleep Assessment Scale (ESS) score, LMT Delayed Recall (LMT DR) score, Rey Osterlieth Complex Graph Test (CFT), Logical Memory Test (LMT), CFT Delayed Recall (CFT DR) score, MMSE score and P300 latency between the two groups were compared, and the abnormal rate and total effective rate of EEG in the two groups after 6 months were counted.
Results:
The apnea hypopnea index (AHI) decreased and LSpO2 increased in conservative group after 6 months of treatment (P<0.05). After 6 months of treatment, AHI and SaO2<90% accounted for the percentage of total monitoring time (TS90%) and (N1+N2) % in the observation group decreased, while LSpO2, N3% and REM sleep period accounted for the percentage of total monitoring time (REM%) increased (P<0.05). The differences of AHI, LSpO2, TS90%, (N1+N2) %, N3% and REM% before and after treatment in observation group were higher than those in conservative group (P<0.05). ESS score in conservative group decreased after 6 months of treatment (P<0.05). After 6 months of treatment, ESS score decreased, MMSE score and MOCA score increased in observation group (P<0.05). The ESS score of observation group was lower than that of conservative group after 6 months of treatment (P<0.05). There was no significant difference in CFT, LMT, and delayed recall scores in the conservative group after 6 months of treatment compared with those before treatment (P>0.05). After 6 months of treatment, the LMT score and the LMT DR Score in the observation group were higher than before, but there was no statistical significance in CFT score and CFT DR Score compared with before treatment (P>0.05). The difference of MMSE score, MOCA score and ESS score before and after treatment in observation group was higher than that in conservative group (P<0.05). There was no significant difference in P300 incubation period in the conservative group after 6 months of treatment compared with before treatment (P>0.05). After 6 months of treatment, the latency of P300 at Fz, Cz, Pz and C4 points decreased, but there was no significant difference in the latency of P300 at C3 points compared with before treatment (P>0.05). The difference of P300 latency before and after treatment in observation group was higher than that in conservative group (P<0.05). After 6 months, the abnormal rate of EEG in the observation group was 24.19% (15/62), which was significantly lower than that in the conservative group (50.00% (31/62), and the total effective rate was 88.71% (55/62), which was significantly higher than that in the conservative group (56.45% (35/62), with statistical difference (P>0.05).
Conclusion:
Modified uvulopalatopharyngoplasty in the treatment of OSAHS with cognitive impairment can effectively improve the sleep structure and EEG activity status of patients, and is conducive to the recovery of cognitive function of patients.
2023 Vol. 29 (9): 1543-1548 [
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44
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1549
Value of Contrast-Enhanced Ultrasound Combined with Serum CA125 VEGF miR-125a and miR-200a in the Early Diagnosis of Endometrial Carcinoma
SUN Hongwei, LI Lijuan, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.025
Objective:
To explore the value of contrast-enhanced ultrasound (CEUS) combined with serum tumor markers in the early diagnosis of endometrial carcinoma (EC).
Methods:
A total of 120 patients with early EC treated in the hospital were enrolled as the research objects between October 2021 and February 2022, while 120 patients undergoing hysteromyoma excision during the same period were enrolled as control group. All underwent transvaginal sonography contrast-enhanced ultrasonography (TVS-CEUS) examination. Taking pathological diagnosis as the golden standard, diagnostic efficiency of CEUS for EC was analyzed. The peripheral blood was collected to detect levels of carbohydrate antigen 125 (CA125) and vascular endothelial growth factor (VEGF). The relative expression levels of serum microRNA-125a (miR-125a) and miR-200a were detected by qRT-PCR. The correlation between CA125, VEGF and miR-125a, miR-200a was analyzed. The diagnostic efficiency of CEUS combined with CA125, VEGF, miR-125a and miR-200a for EC was evaluated by receiver operating characteristic (ROC) curves.
Results:
The sensitivity, specificity, accuracy, positive predictive value, negative predictive value and kappa value of TVS-CEUS in the early diagnosis of EC were 81.67%, 71.67%, 76.67%, 74.24%, 79.63%, and 0.533, respectively. The levels of serum CA125 and VEGF, and relative expression level of miR-200a in EC group were higher than those in control group, while relative expression level of miR-125a was lower than that in control group (P<0.05). The results of Pearson correlation analysis showed that levels of serum CA125 and VEGF were negatively correlated with relative expression level of miR-125a (P<0.05), while positively correlated with relative expression level of miR-200a (P<0.05). The result of ROC curve analysis showed that AUC of TVS-CEUS combined with CA125, VEGF, miR-125a, and miR-200a in the early diagnosis of EC was significantly greater than that of CA125, VEGF, miR-125a, and miR-200a alone (P<0.05).
Conclusion:
TVS-CEUS combined with serum tumor markers has high early diagnostic efficiency for EC, which can provide reference for condition evaluation of patients.
2023 Vol. 29 (9): 1549-1553 [
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27
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1554
Effects of Electronic Bronchoscopy-Guided Stent Placement on Blood Gas Indexes Shortness of Breath and Pulmonary Function in Patients with Malignant Airway Stenosis
LUO Yukun, LIU Xin, GAO Liyu, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.026
Objective:
To observe the effects of electronic bronchoscopy-guided stent placement on blood gas indexes, shortness of breath, and pulmonary function in patients with malignant airway stenosis.
Methods:
A total of 120 patients with malignant airway stenosis who were treated with electronic bronchoscopy-guided stent placement were included in this study. The success rate of one-time placement, complications and 6-month postoperative survival were observed. Blood gas indexes [partial pressure of oxygen (PaO
2
), partial pressure of carbon dioxide (PaCO
2
) and oxygen saturation (SaO
2
)], classification of shortness of breath, pulmonary function indicators [forced expiratory volume in 1s (FEV1), forced vital capacity (FVC) and peak expiratory flow (PEF)], and ECOG score were compared before and after treatment.
Results:
The success rate of one-time placement was 96.67%. After operation, there were 3 patients with obvious bloody phlegm, 9 patients with a little blood in sputum, and 8 patients with mild substernal pain, which all disappeared after symptomatic treatment. After treatment, dyspnea in all patients was relieved significantly. 21 (17.5%) patients died from the primary disease in 6 months after operation. After treatment, PaO
2
and SaO
2
increased significantly (P<0.05), and PaCO
2
decreased significantly (P<0.05). Shortness of breath was significantly improved (P<0.05). FEV1, FVC and PEF increased significantly (P<0.05), and ECOG score decreased significantly (P<0.05).
Conclusion:
Electronic bronchoscopy-guided stent placement is safe and effective in the treatment of malignant airway stenosis. It can significantly improve blood gas indexes, shortness of breath and pulmonary function.
2023 Vol. 29 (9): 1554-1558 [
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36
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1559
The Relationship between C-reactive Protein and Non-Invasive Ventilation in Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease Complicated with Type II Respiratory Failure
YANG Yi, LI Qun, HE Mengjun, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.027
Objective:
To investigate the relationship between the levels of C-reactive protein (CRP), suppression of tumorigenicity 2 (ST2), procalcitonin (PCT), lactic acid, and non-invasive ventilation in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) complicated with respiratory failure.
Methods:
A total of 146 patients with AECOPD and type II respiratory failure treated in our hospital from January 2021 to June 2022 were selected as the observation group, and 100 patients with simple AECOPD were selected as the control group. The levels of CRP, ST2, PCT and lactic acid in the observation group and the control group were compared. The observation group was given non-invasive ventilation treatment, and the clinical data, CRP, ST2, PCT and lactic acid levels of patients with treatment failure and success were compared.
Results:
The levels of CRP, ST2, PCT and lactic acid in the observation group were significantly higher than those in the control group (P<0.05). The course of COPD, APACHE Ⅱ score, fasting blood glucose, CRP, ST2, PCT and lactic acid levels of patients with treatment failure in the observation group were significantly higher than those of patients with treatment success (P<0.05). Logistic regression analysis showed that APACHE Ⅱ score, CRP, ST2, PCT and lactic acid were the influencing factors for the failure of noninvasive ventilation treatment (P<0.05). The area under the ROC curve of APACHE Ⅱ score, CRP, ST2, PCT, lactic acid and the combination of them to predict treatment failure were 0.662, 0.698, 0.717, 0.658, 0.759 and 0.806 respectively (P<0.05).
Conclusion:
The levels of CRP, ST2, PCT and lactic acid in AECOPD patients with type II respiratory failure increased significantly. The evaluation based on the patient's disease progress and APACHE Ⅱ score, CRP, ST2, PCT and LCR can provide a more comprehensive reference and basis for predicting the effect of ventilation treatment.
2023 Vol. 29 (9): 1559-1563 [
Abstract
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54
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1564
Changes in TC/HDL, ApoA1 and Lp(a) Levels in Patients with Schizophrenia and Their Relationship with Metabolic Syndrome
LU Wen, GONG Chen, XIANG Wen, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.028
Objective:
To explore the changes in total cholesterol/high density lipoprotein (TC/HDL), apolipoprotein A1 (ApoA1) and lipoprotein (a) [Lp(a)] and their relationship with metabolic syndrome (MS) in patients with schizophrenia.
Methods:
A total of 93 patients with schizophrenia in the hospital were selected as the study subjects. General data of patients were counted. The levels of serum TC/HDL, ApoA1 and Lp(a) were detected by enzyme-linked immunosorbent assay, and the patients were divided into MS group (n=32) and non-MS group (n=61) according to whether they were complicated with MS. The general data and levels of serum TC/HDL, ApoA1 and Lp(a) were compared between the two groups. Multivariate Logistic regression analysis was used to analyze the risk factors of MS in patients with schizophrenia.
Results:
The BMI, SBP, DBP, FPG, serum TG and LDL-C in MS group were significantly higher than those in non-MS group (P<0.05) while the level of HDL-C was significantly lower than that in non-MS group (P<0.05). There were no statistically significant differences in gender, age, course of disease, smoking history, drinking history, antipsychotic drug use, serum TC level, and PANSS score between the two groups (P>0.05). TC/HDL and Lp(a) in MS group were significantly higher than those in non-MS group (P<0.05) while ApoA1 was significantly lower than that in non-MS group (P<0.05). Multivariate Logistic regression analysis showed that BMI, FPG, serum TG, LDL-C, Lp(a) and TC/HDL were risk factors affecting MS in patients with schizophrenia, and serum ApoA1 was a protective factor (P<0.05).
Conclusion:
Serum TC/HDL, ApoA1 and Lp (a) levels are the influencing factors of MS in patients with schizophrenia, and the increases of TC/HDL and Lp (a) and the decrease of ApoA1 can increase the risk of MS in patients.
2023 Vol. 29 (9): 1564-1568 [
Abstract
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41
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1569
Application of Xueshuantong Capsule Combined with Conbercept in Patients with Central Retinal Vein Occlusion and Macular Oedema
TANG Huichao, LI Man, WANG Dongmei, et al
DOI: 10.3969/j.issn.1006-6233.2023.09.029
Objective:
To explore the application effect of Xueshuantong capsule combined with Conbercept in patients with central retinal vein occlusion (CRVO) and macular oedema.
Methods:
A total of 90 patients with ischemic CRVO and macular oedema were enrolled between October 2019 and September 2021. According to simple random grouping method, they were divided into group A (45 cases, 45 eyes, Conbercept combined with Xueshuantong capsules) and group B (45 cases, 45 eyes, Conbercept). All were treated for 3 months. The onset time of visual acuity, retinal circulation time, central macular thickness (CMT), fundus hemodynamics, best corrected visual acuity (BCVA), serum indexes and adverse reactions in the two groups were compared.
Results:
The response rate of treatment in the two groups was similar (χ
2
=0.179, P>0.05). Compared with group B, onset time of visual acuity was shorter in group A (t=2.237, P<0.05). After treatment, retinal circulation time was similar in the two groups (t=1.149, P>0.05). After 2 and 3 months of treatment, CMT was thinner in group A than group B (t=3.884, 2.104, P<0.05). Compared with group B after treatment, increments of peak systolic velocity and end-diastolic velocity were greater in group A, while decrements of resistance index and pulsatility index were greater in group A (t=7.240, 6.452, 6.202, 6.442, P<0.05). After 1 month of treatment, BCVA log MAR was lower in group A than group B (t=2.472, P<0.05). Compared with group B after treatment, increment of serum nitric oxide synthetase was greater in group A, while decrements of interleukin-6 and vascular endothelial growth factor were greater in group A (t=7.799, 15.034, 4.973, P<0.05). The incidence of adverse reactions was similar in the two groups (P>0.05).
Conclusion:
Compared with Conbercept alone, Conbercept combined with Xueshuantong capsule is conducive to the relief of macular edema, improvement of retinal microcirculation, and vision recovery in CRVO.
2023 Vol. 29 (9): 1569-1574 [
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33
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