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2023 Vol. 29, No. 4 Published: 2023-04-30 529 The Role of miR-30a-3p in the Migration and Angiogenesis Ability of Renal Cancer Cells through Targeted Regulation of BAFF WEILIJIANG·Saimaiti, MA Jun, WANG Yujie DOI: 10.3969/j.issn.1006-6233.2023.04.01 Objective: To investigate the effects of microRNA (miR)-30A-3p on migration and angiogenic phenotype of renal cell carcinoma (RCC) cells. Methods: The expression characteristics of miR-30a-3p in RCC cells were analyzed based on the expression data of miR-30a-3p in the Cancer Genome Atlas (TCGA) database. Predict the binding of miR-30a-3p to the 3'untranslated region (3' -UTR) of B cell activicting factor (BAFF) by bioinformatics websites. RCC cell lines ACHN and 786O were divided into LV-miR-30a-3p group [infected with lentiviral vector overexpressing miR-30a-3p], LV-NC group [infected with lentiviral vector negative control], LV-miR-30a-3p+pcDNA-BAFF group [co-infected with LV-miR-30a-3p and LV-Mir-30a-3p Overexpressing BAFF vector (pcDNA-BAFF)] and LV-miR-30a-3p+pcDNA-EV group [co-infected with LV-miR-30a-3p and empty vector (pcDNA-EV)]. Transwell migration chamber assay was used to detect cell migration ability, and tubule formation assay was used to detect cell angiogenesis ability. The expression of miR-30a-3p was detected by qPCR. The protein expressions of BAFF and vascular endothelial growth factor (VEGFA) were detected by Western blot. Targeting regulation of miR-30a-3p against BAFF in ACHN cells was detected by dual luciferase gene reporting assay. Results: Analysis of the data in the TCGA database showed that miR-30a-3p expression was significantly lower in the Cancer group compared to the Normal group (P<0.05). Compared with HK-2 cells, the expression of miR-30a-3p in ACHN and 786O cell lines was significantly decreased (both P<0.05). Compared with the LV-NC group, the expression of miR-30a-3p was increased in the LV-miR-30a-3p group (all P<0.05), while the cell mobility, tubule formation rate, and the expressions of BAFF and VEGFA were down-regulated in the LV-miR-30a-3p group (all P<0.05). According to bioinformatics prediction and double luciferase reporter assay, BAFF was found to be the target gene of miR-30a-3p. Compared with the LV-miR-30a-3p+pcDNA-EV group, cell mobility, tubule formation rate and BAFF expression in the LV-miR-30a-3p+pcDNA-BAFF group were up-regulated (all P<0.05). Conclusion: miR-30a-3p inhibits the migration and angiogenesis of RCC cells by targeting inhibits BAFF. 2023 Vol. 29 (4): 529-535 [Abstract] ( 110 ) HTML (1 KB)  PDF (2706 KB)  ( 101 ) 535 Effects of Quercetin on Cell Biological Behaviors Including Proliferation Apoptosis and p38/JNK MAPK Signaling Pathway in Oral Squamous Carcinoma Cells HU Shuohong, ZHENG Xuebin, LI Fujie DOI: 10.3969/j.issn.1006-6233.2023.04.02 Objective: To investigate the effect of quercetin on the proliferation, apoptosis, migration, invasion and p38/JNK MAPK signaling pathway of oral squamous cell carcinoma cells. Methods: Oral squamous cell carcinoma Tac8113 cells cultured in vitro were used as research subjects, and blank control group, low-dose quercetin group (25μmol/L), high-dose quercetin group (50μmol/L) and positive control group (cisplatin 4μg/mL) were set respectively. The effect of quercetin on the proliferation of oral squamous cell carcinoma Tac8113 cells was detected by CCK-8 method; the effect of quercetin on the apoptosis of oral squamous cell carcinoma Tac8113 cells was detected by flow cytometry; the migration and invasion of oral squamous cell carcinoma Tac8113 cells was detected by Transwell assay; WB method was used to detect the expression of p38/JNK MAPK signaling pathway-related proteins. Results: Compared with the blank control group, the proliferation activity, migration number and invasion number of Tac8113 cells in the low-dose and high-dose quercetin groups and the positive control group decreased, and the apoptosis rate increased (P<0.05). Compared with Tac8113 cells in the high-dose quercetin group and the positive control group, the proliferation activity, the number of migration and the number of invasion decreased, and the apoptosis rate increased (P<0.05), in a dose-dependent manner; There was no significant difference in the proliferation activity, migration number, invasion number and apoptosis rate of Tac8113 cells between the high-dose quercetin group and the positive control group. (P>0.05). Compared with the blank control group, the expressions of p-p38, p-JNK and Caspase-3 in the low-dose and high-dose quercetin groups were increased (P<0.05); compared with the low-dose quercetin group, the protein expressions of p-p38, p-JNK and Caspase-3 in the high-dose quercetin group were increased (P<0.05), in a dose-dependent manner; The differences in p38 and JNK protein expression among the blank control group, quercetin low dose group, quercetin high dose group, and positive control group were not significant (P>0.05). Conclusion: Quercetin can reduce the proliferation activity, migration and invasion number of oral squamous cell carcinoma Tac8113 cells, and induce Tac8113 cell apoptosis, which may be achieved by activating the p38/JNK MAPK signaling pathway. 2023 Vol. 29 (4): 535-540 [Abstract] ( 56 ) HTML (1 KB)  PDF (2134 KB)  ( 125 ) 540 Database-Based Exploration of Abnormal Expression and Prognosis of Centromeric Protein F in Lung Adenocarcinoma ZHOU Meifeng, WANG Lin, WANG Haixia, et al DOI: 10.3969/j.issn.1006-6233.2023.04.03 Objective: To explore the expression of Centromeric protein F(CENPF)and its biological function in LUAD. Methods: CENPF mRNA expression in LUAD was analyzed by searching the Oncomine database, CENPF protein expression levels were determined by immunohistochemical (IHC) staining using the HPA database, and IHC images were obtained from the HPA database. In addition, subgroups and prognosis of LUAD were analyzed by using the Ualcan and GEPIA database tools, respectively. To further explore the CENPF sequence mutations in LUAD patients, the c-Bioportal website was applied. Finally, the LinkedOmics database was applied to analyze the co-expressed genes of CENPF in the LUAD cohort for functional and pathway analysis by the LinkInterpreter module. Results: CENPF was highly expressed in LUAD (P<0.05), which could lead to poor prognosis by Kaplan-Meier analysis (P<0.05). And CENPF mRNA expression was related to smoking status, TP53 mutation and tumor stage in LUAD patients (P<0.05). In addition, CENPF was mutated in LUAD, but it had no effect on LUAD prognosis (P=0.698). Co-expression analysis showed that CENPF co-expressed genes were closely related to cell cycle and cell division process. Conclusion: CENPF can be involved in the development of LUAD as a pro-oncogene and has potential applications as a diagnostic, prognostic indicator and targeted therapy for LUAD. 2023 Vol. 29 (4): 540-545 [Abstract] ( 68 ) HTML (1 KB)  PDF (2932 KB)  ( 214 ) 545 Effect of miR-153-3p Targeted Regulation of CHI3L1 on the Inflammatory Response of LPS-Induced Human Periodontal Ligament Stem Cells FAN Jing, et al DOI: 10.3969/j.issn.1006-6233.2023.04.04 Objective: To explore whether miR-153-3p can improve the inflammatory response of human periodontal ligament stem cells (hPDLSCs) induced by lipopolysaccharide (LPS) by targeting regulates chitinase 3-like protein 1 (CHI3L1). Methods: The hPDLSCs were isolated and cultured, and inflammatory cell models were established using LPS treatment and transfected with miR-NC, miR-153-3p mimics, si-NC, si-CHI3L1, and miR-153-3p mimics+pc-NC, miR-153-3p mimics+pcDNA-CHI3L1. 48 h after transfection, the expressions of miR-153-3p and CHI3L1 mRNA in the cells of each group were detected by qRT-PCR, the levels of IL-6, IL-1β and TNF-α in the cell supernatant were detected by ELISA, cell apoptosis was detected by flow cytometry, the expression of CHI3L1 protein in cells was detected by Western Blot, the targeting relationship between miR-153-3p and CHI3L1 was detected by dual-luciferase reporter gene assay. Results: The expression of miR-153-3p was down-regulated in hPDLSCs after LPS treatment (P<0.05), while the expressions of CHI3L1 mRNA and protein were significantly up-regulated (P<0.05); over-expression of miR-153-3p or inhibition of CHI3L1 expression could reduce the levels of IL-6, IL-1β and TNF-α in LPS-induced hPDLSCs, down-regulate the expressions of CHI3L1 mRNA and protein, and reduce the apoptosis rate, and the differences were statistically significant (P<0.05). The dual-luciferase reporter gene assay confirmed that miR-153-3p could targeting regulates the expression of CHI3L1 (P<0.05). In addition, the over-expression of CHI3L1 could significantly reverse the inhibitory effects of the over-expression of miR-153-3p on LPS-induced apoptosis and inflammatory response of hPDLSCs (P<0.05). Conclusion: MiR-153-3p can reduce the LPS-induced inflammatory response and apoptosis of hPDLSCs by targeting regulates CHI3L1 expression. 2023 Vol. 29 (4): 545-551 [Abstract] ( 35 ) HTML (1 KB)  PDF (1915 KB)  ( 228 ) 551 Bioinformatics-Based Analysis of the Expression and Significance of SUFU Gene in Gastric Cancer Tissues YANG Bo, HE Lei, FU Tianli, et al DOI: 10.3969/j.issn.1006-6233.2023.04.05 Objective: Multiple databases combined with real-time PCR assay were used to analyze the expression of SUFU gene in gastric cancer tissues, and to explore its clinical significance. Methods: The Oncomine database was used to analyze the expression of SUFU gene in gastric cancer, and UALCAN, an online tool of TGCA database, was used to analyze the difference of SUFU gene expression between normal gastric tissues and gastric cancer tissues and the relationship between SUFU gene expression and clinicopathological features of gastric cancer. Kaplan-Meier Plotter database was used to analyze the correlation between SUFU gene expression and prognosis of gastric cancer patients, and clinical samples were collected and verified by PCR experiment. Results: Meta-analysis of 12 studies on gastric cancer in Oncomine database showed that the median ranking of SUFU differentially expressed genes was 1560.0 (P=0.002), among which 5 studies showed that the expression of SUFU in gastric cancer tissues was significantly higher than that in adjacent normal tissues. UALCAN also showed that SUFU was highly expressed in gastric cancer tissues, and the high expression of SUFU was correlated with the age of patients and the degree of tumor differentiation. The expression level of SUFU in the younger group was higher than that in the older group. The expression level of SUFU in low differentiation group was higher than that in high differentiation group and medium differentiation group (P<0.05). Kaplan-Meier Plotter database showed that the expression level of SUFU was significantly correlated with the prognosis of gastric cancer patients (P<0.05), the overall survival rate of patients with high SUFU expression was lower than that of patients with low SUFU expression. PCR also verified that SUFU gene was highly expressed in gastric cancer tissues. Conclusion: SUFU is highly expressed in gastric cancer tissues and is associated with poor prognosis in gastric cancer patients. 2023 Vol. 29 (4): 551-555 [Abstract] ( 86 ) HTML (1 KB)  PDF (2209 KB)  ( 184 ) 555 Research LTC4S Gene Polymorphisms on the Efficacy of Montelukast in the Treatment of Bronchial Asthma in Han Chinese Children WANG Shanshan, et al DOI: 10.3969/j.issn.1006-6233.2023.04.06 Objective: To investigate the effect of single nucleotide polymorphism (SNP) at rs730012 of leukotriene C4 synthase (LTC4S) gene on the efficacy of Montelukast in the treatment of bronchial asthma in Han Chinese children. Methods: A total of 100 children with bronchial asthma aged 4 to 13 years were selected from the Department of Pediatrics of Xiamen Hospital of Traditional Chinese Medicine from April 2019 to December 2021. The rs730012 site of LTC4S gene in all children was detected by generation sequencing method, and the SNP in this site was compared. At the same time, all enrolled children were treated with Montelukast. Pulmonary ventilation function and pulse shock lung function were detected and clinical control level was evaluated before treatment and at 6th week and 12th week of treatment, respectively, to analyze the correlation between SNP and efficacy. Results: LTC4S (rs730012) gene was divided into AA, AC and CC, and the distribution of each genotype was consistent with genetic balance. After 6 weeks and 12 weeks of treatment, the good control rate of clinical symptoms in children with AA and AC were better than those with CC, and the difference was statistically significant (P<0.05). The differences in each index of ventilatory lung function and pulsed shock lung function at 6 weeks of treatment in children with type AA were statistically significant compared with those before treatment (P<0.05).At 12 weeks of treatment, there were statistically significant differences in the airway ventilation lung function indexes FEV1, PEF, FVC and pulse shock lung function indexes Zat5, Rat5, Rat20, Rat10, Rat15 and resonance frequency compared with those before treatment (P<0.05). Zat5, Rat5, Rat10 at 6 weeks of treatment and Zat5, Rat5, Rat10, Rat15, resonance frequency improvement at 12 weeks of treatment were better than before treatment for AC type (P<0.05), but the difference in ventilation lung function index was not statistically significant compared with before treatment (P>0.05). There were only 3 cases of CC type in this study, and the comparison was not statistically significant. Conclusion: The SNP at the LTC4S (rs730012) locus was correlated with the efficacy of montelukast, with individuals carrying the A allele having better efficacy against montelukast. 2023 Vol. 29 (4): 555-560 [Abstract] ( 51 ) HTML (1 KB)  PDF (1257 KB)  ( 122 ) 561 Effect of High Flux Dialysis Combined with Hemoperfusion on Nutritional Status and Calcium-Phosphorus Metabolism in Maintenance Hemodialysis Patients TANG Yueqiu, LU Xiaomei DOI: 10.3969/j.issn.1006-6233.2023.04.07 Objective: To investigate the effect of high flux dialysis (HFD) combined with hemoperfusion (HP) on nutritional status and calcium-phosphorus metabolism in maintenance hemodialysis (MHD) patients. Methods: A total of 87 patients who underwent MHD in the 363 Hospital from January 2019 to January 2022 were selected, and randomly divided into 2 groups. Patients in the observation group (n=44) were treated with HFD combined with HP, and patients in the control group (n=43) were treated with conventional hemodialysis. Both groups received 3 consecutive months of treatment. The two groups were compared on nutritional status, inflammatory status, calcium-phosphorus metabolism level, and sleep quality before and after treatment, and adverse reactions were recorded. Results: After treatment, the levels of serum albumin (ALB), prealbumin (PA), hemoglobin (HB) and body mass index (BMI) were increased, while the levels of interleukin-6 (IL-6), high-sensitivity C-reactive protein (hs-CRP), tumor necrosis factor (TNF-α), blood calcium, blood phosphorus and intact parathyroid hormone (iPTH), calcium-phosphorus product and Pittsburgh Sleep Quality Index (PSQI) were significantly reduced. The changes of serum ALB, PA, HB, IL-6, hs-CRP, TNF-α, blood calcium, blood phosphorus, iPTH, calcium-phosphorus product and PSQI scores in the observation group before and after treatment were greater than those in the control group (P<0.05). The incidence rates of skin pruritus, myalgia and fatigue in the observation group (9.09%, 11.36% and 9.09%) were lower than those in the control group (30.23%, 32.56% and 25.58%) (P<0.05). Conclusion: HFD combined with HP can effectively improve the nutritional status of MHD patients, correct micro-inflammatory state and calcium-phosphorus metabolism, improve sleep quality, and reduce adverse reactions. 2023 Vol. 29 (4): 561-566 [Abstract] ( 71 ) HTML (1 KB)  PDF (1245 KB)  ( 188 ) 566 Comparison of the Efficacy of Three Different Approaches in Treating Thoracolumbar Fractures MENG Li, SUN Yuliang, WAN Dun, et al DOI: 10.3969/j.issn.1006-6233.2023.04.08 Objective: To investigate the efficacy of three different approaches in the treatment of thoracolumbar fractures. Methods: The clinical data of 325 patients with thoracolumbar fractures admitted to our hospital from January 2020 to January 2021 were retrospectively analyzed. The patients treated with the posterior median approach, percutaneous approach, and paravertebral interbody approach with the pedicle nail rod system were included in groups A (n=110), B (n=108), and C (n=107), respectively, and the clinical efficacy, perioperative indexes, injured spine indexes, and prognosis of the patients in the three groups were compared. The clinical outcomes, perioperative indexes, injured spine indexes and prognosis of the three groups were compared. Results: VAS scores and ODI scores decreased in all three groups at 3 months after surgery, and the difference between the preoperative and postoperative values of patients in the three groups were compared, and the changes in groups B and C were greater than those in group A and the changes in group C were greater than those in group B. The differences in the changes in VAS scores and ODI scores in the three groups were statistically significant (P<0.05); the difference in operating time, intraoperative bleeding, hospitalization time and fracture healing time was less in groups B and C than in the control group; the incision length was shorter in group B than in groups A and C (P<0.05); the posterior convexity Cobb's angle decreased and the height of the anterior border of the injured spine increased in all three groups at 3 months after surgery. Comparing the changes in the difference between the three groups of patients before and 3 months after surgery, the changes in groups B and C were greater than those in group A and the changes in group C were greater than those in group B. There were statistically significant differences in the magnitude of changes in the posterior convex Cobb angle and the height of the anterior edge of the injured spine in the three groups (P<0.05); there were no statistically significant differences in the three-dimensional range of motion in the three groups (P>0.05); patients in all three groups had complications such as incisional infection and postoperative residual low back pain, and there were no statistically significant differences in the incidence of the three groups (P>0.05). Conclusion: The three surgical approaches have good prognosis and can improve spinal motion to some extent, but the percutaneous approach and the paravertebral interbody approach improve the efficacy, perioperative indexes and the level of injured spine indexes more significantly in patients with thoracolumbar fractures. The clinical choice of surgical approach should be targeted to enhance the therapeutic benefit. 2023 Vol. 29 (4): 566-572 [Abstract] ( 52 ) HTML (1 KB)  PDF (2714 KB)  ( 98 ) 572 Analysis of the Current Situation of Acquired Legionella Infection and Its Risk Factors in a Tertiary Care Hospital in Guangxi DING Kaihong, FENG Yuqing, TANG Fangmei, et al DOI: 10.3969/j.issn.1006-6233.2023.04.09 Objective: To investigate and analyze the current status of acquired Legionella infection and its risk factors in a tertiary care hospital in Guangxi. Methods: 480 inpatients in a tertiary care hospital in Guangxi from June 2021 to June 2022 were selected, Legionella infections were counted, the influencing factors of Legionella infections were analyzed by single-factor and logistic multi-factor models, a logistic prediction model was constructed based on the influencing factors, and the value of the model in predicting the occurrence of Legionella infections was evaluated by subject work characteristic (ROC) curves. Results: The rate of Legionella infection was 12.08% (58/480) in 480 hospitalized patients, of which 41.38% (32/58) were combined with other bacterial or fungal infections; the age of patients who developed Legionella infection and the proportion of patients who smoked, had diabetes, had respiratory diseases, were admitted in summer and autumn, had recently used centralized air conditioning, and applied immunosuppressive drugs were higher than those who did not develop Legionella infection (P<0.05). Age (OR=1.633), smoking (OR=2.297), diabetes (OR=2.512), respiratory disease (OR=5.118), admission in summer and autumn (OR=5.638), recent use of centralized air conditioning (OR=4.710), and application of immunosuppressive drugs (OR=3.933) were all factors influencing Legionella infection (P<0.05). The logistic prediction model was constructed based on the influencing factors as: Logit (P) = -8.054 + 0.490 × age + 0.832 × smoking + 0.921 × diabetes mellitus + 1.633 × respiratory disease + 1.730 × hospital admission in summer and autumn + 1.550 × recent use of centralized air conditioning + 1.369 × application of immunosuppressive drugs. The model predicted the occurrence of Legionella infection with an area under the curve (AUC) of 0.918 (95% CI 0.890 to 0.941), a cut-off value of 0.163, and a predictive sensitivity and specificity of 89.67% and 84.65%, respectively. Conclusion: The incidence of acquired Legionella infection in a tertiary care hospital in Guangxi was 12.08%, and the influencing factors included age, smoking, diabetes, respiratory diseases, admission in summer and autumn, recent use of centralized air conditioning, and application of immunosuppressive drugs, and the construction of a Logistic prediction model based on the influencing factors could assist in clinical screening of patients at high risk of Legionella infection. 2023 Vol. 29 (4): 572-578 [Abstract] ( 63 ) HTML (1 KB)  PDF (1300 KB)  ( 127 ) 578 Level of Serum 25-Hydroxyvitamin D and Its Correlation with IL-8 TNF-α and TLR2 in Patients with Different Severity of Acne ZHANG Xiaofang, GAO Jun, JIANG Hui, et al DOI: 10.3969/j.issn.1006-6233.2023.04.010 Objective: To explore the correlation between serum 25-hydroxyvitamin D and interleukin-8 (IL-8), tumor necrosis factor (TNF-α), Toll-like receptor 2 (TLR2) in patients with different severity of acne. Methods: A total of 218 patients with acne admitted to the hospital were enrolled as acne group between October 2019 and December 2021. All underwent serological tests. According to pillsbury grading, they were divided into grade I group, grade II group, grade III group and grade IV group. A total of 218 healthy controls during the same period were randomly enrolled as control group. The levels of serum IL-8, TNF-α, TLR2 and 25-hydroxyvitamin D were compared between acne group and control group, and among patients with different pillsbury grading. The correlation between serum 25-hydroxyvitamin D and IL-8, TNF-α, TLR2 was analyzed by Pearson correlation analysis. The correlation between pillsbury grading and serum IL-8, TNF-α, TLR2, 25-hydroxyvitamin D was analyzed by Spearman correlation analysis. Results: The levels of serum IL-8, TNF-α, TLR2 and 25-hydroxyvitamin D in acne group were significantly higher than those in control group (P<0.05). The levels of serum IL-8, TNF-α, TLR2 and 25-hydroxyvitamin were the highest in grade IV group, followed by grade III group, grade II group and grade I group (P<0.05). Pearson correlation analysis showed that level of serum 25-hydroxyvitamin D was positively correlated with levels of serum IL-8, TNF-α and TLR2 (P<0.05). Spearman correlation analysis showed that pillsbury grading was positively correlated with levels of serum IL-8, TNF-α, TLR2 and 25-hydroxyvitamin D (P<0.05). Conclusion: Serum 25-hydroxyvitamin D levels in acne patients are correlated with inflammatory factors and acne severity and can be used for clinical assessment of disease progression. 2023 Vol. 29 (4): 578-582 [Abstract] ( 56 ) HTML (1 KB)  PDF (1293 KB)  ( 74 ) 582 Clinical Efficacy and Safety Analysis of Emaglutide in Overweight and Obese Patients with Type 2 Diabetes Mellitus LI Yanni, LIU Hongli, WANG Yeju, et al DOI: 10.3969/j.issn.1006-6233.2023.04.011 Objective: To investigate the clinical efficacy and safety of smegliptide in overweight or obese (T2DM) patients with type 2 diabetes mellitus. Methods: Eighty overweight or obese patients with T2DM admitted in Hanzhong Central Hospital, Shaanxi Province from January 2022 to June 2022 were enrolled for the study and divided into observation group (n = 40) and control group (n = 40) through simple random grouping method. On the basis of diet control and aerobic exercise, patients in the control group and observation group were metformin, metformin + smeglutide respectively. The blood glucose, body weight, blood lipid, blood pressure and adverse reactions were compared between the two groups before intervention and 12 weeks after intervention. Results: Compared with the level before the intervention within group, the fasting plasma glucose (FPG), 2-hour postprandial plasma glucose (2hPPG), glycosylated hemoglobin (HbAlc), homeostasis model assessment of insulin resistance index (HOMA-IR), body mass index (BMI), total triglyceride (TG), total cholesterol (TC), systolic blood pressure (SBP) and diastolic blood pressure (DBP) in the two groups significantly decreased after the intervention, and the homeostasis model assessment of islet β cell function index (HOMA-β) significantly increased (P<0.05). Compared with the control group, the difference of FPG, 2hPPG, HbAlc, HOMA-IR, HOMA-β, BMI, SBP, DBP, TG, TC before and after intervention in observation group significantly increased (P<0.05). There were no significant differences in the incidences of adverse reactions between two groups (P>0.05). Conclusion: Smegliptide could not only significantly reduce the blood glucose level of T2DM patients with overweight or obese, but also strengthen the control of body weight, blood lipid and blood pressure, improve insulin resistance. It is safe, reliable and worthy of clinical application. 2023 Vol. 29 (4): 582-587 [Abstract] ( 176 ) HTML (1 KB)  PDF (1254 KB)  ( 402 ) 587 Effects of Extracorporeal Shock Wave Therapy Combined with Lower Limb Muscle Strength Training on Joint Stability Range of Motion and Balance Function in Patients after Arthroscopic Anterior Cruciate Ligament Reconstruction ZHANG Yi, GU Yuanyuan, QU Jingzhi, et al DOI: 10.3969/j.issn.1006-6233.2023.04.012 Objective: To investigate the effect of extracorporeal shock wave therapy (ESWT) combined with lower limb muscle strength training on joint stability, range of motion (ROM) and balance function of patients after arthroscopic anterior cruciate ligament reconstruction (ACLR). Methods: 92 post-arthroscopic ACLR patients admitted to Hospital 983 of the Joint Security Force between January 2020 and September 2021 were selected for the study and randomly divided into the observation group (n=46) and the control group (n=46), the control group was given conventional post-operative lower limb muscle strength training, and the patients in the observation group were given ESWT on top of the above for 5 weeks. The difference in the distance of posterior anterior tibial movement between the affected side and the healthy side before and 10 weeks after the intervention (hereinafter referred to as "tibial anterior displacement difference"), the knee ROM, the knee function score of the international knee documentation committee (IKDC) and the Berg Balance Scale (BBS) were compared between the two groups. Results: In the intra-group comparison, patients in both groups had lower tibial anterior displacement difference after the intervention than before the intervention, and higher knee ROM, IKDC and BBS scores than before the intervention (P<0.05). For comparison between groups, the difference in tibial anterior displacement of patients in the observation group after intervention was lower than that of the control group during the same period, and the difference in knee ROM, IKDC and BBS scores and each index before and after intervention was higher than that of the control group (P<0.05). Conclusion: ESWT combined with lower limb muscle strength training could significantly improve the knee joint stability, ROM and balance function of patients after arthroscopic ACLR, which is worthy of clinical application. 2023 Vol. 29 (4): 587-591 [Abstract] ( 74 ) HTML (1 KB)  PDF (1231 KB)  ( 106 ) 591 Analysis of Clinical Characteristics and Factors Associated with the Occurrence of Sarcopenia in Elderly Patients after Esophageal Squamous Carcinoma Surgery WANG De’en, YANG Wei, WANG Weimin, et al DOI: 10.3969/j.issn.1006-6233.2023.04.013 Objective: To analyze the clinical characteristics and related factors of sarcopenia (SA) in elderly patients after esophageal squamous carcinoma surgery. Methods: A retrospective analysis was performed on the clinical data of 120 elderly patients with esophageal squamous carcinoma admitted to the hospital between March 2018 and October 2021. According to L3 skeletal muscle index (L3 SMI) and international diagnostic criteria for SA (male: L3 SMI ≤40.8cm2/m2, female: L3 SMI ≤34.9cm2/m2), the patients were divided into SA group (n=49) and non-SA group (n=71). Postoperative clinical characteristics of the two groups were recorded, and the influencing factors of SA were screened by logistic regression analysis. Results: There were statistically significant differences in general data [age, Karnofsky Performance Scale (KPS) score, body mass index (BMI), and serum prealbumin (PA)] between the two groups (P<0.05), but there was no statistically significant difference in the other clinical indexes (P>0.05). Logistic regression analysis showed that age, KPS score and BMI were influencing factors of SA. Conclusion: The occurrence of SA in elderly patients after esophageal squamous carcinoma surgery is related to multiple factors. Clinically, attention should be paid to the related factors of SA, timely and targeted intervention measures should be taken to reduce the incidence of postoperative SA. 2023 Vol. 29 (4): 591-595 [Abstract] ( 59 ) HTML (1 KB)  PDF (1223 KB)  ( 115 ) 595 Effectiveness of Enteric Probiotic Supplementation in the Treatment of Children with Bronchial Asthma and Its Mechanism of Action MA Qibo, ZHAN Feng, ZHONG Chong, et al DOI: 10.3969/j.issn.1006-6233.2023.04.014 Objective: To investigate the effect of enteric probiotics supplementation in children with bronchial asthma and its mechanism of action. Methods: A total of 105 children with bronchial asthma admitted to our hospital from January 2019 to January 2022 were randomly divided into routine group (52 cases) and probiotic group (53 cases). The routine group was given routine treatment, and the probiotic group was given intestinal probiotics adjuvant treatment on the basis of routine treatment. The two groups were detected the differences of intestinal flora, immune function and sputum eosinophil percentage (EOS%), integrin α4β1 and αvβ6 before and after treatment, and the adverse effects and the number of acute attacks within 6 months were counted. Results: Before treatment, there was no significant difference in intestinal flora between the two groups (P>0.05). After treatment, bifidobacterium and lactobacillus in probiotics group were higher than before, while Escherichia coli was lower. There was no statistical significance in intestinal flora in conventional group compared with before treatment (P>0.05). The difference values of bifidobacterium, lactobacillus and Escherichia coli before and after treatment in probiotic group were larger than those in conventional group (P<0.05). Before treatment, there were no significant differences in sputum EOS%, integrin α4β1, αvβ6 and immune function between the two groups (P>0.05). After treatment, CD8 Anhui+, EOS% of sputum, integrin α4β1, αvβ6 were decreased, and CD4 Anhui+, CD4 Anhui+/CD8 Anhui+ were increased in both groups (P<0.05). The difference before and after treatment in probiotic group was larger than that in conventional group (P<0.05). There were 3 cases of adverse reaction in probiotics group, the incidence was 5.66%, and the adverse reaction rate was not statistically significant compared with 11.54% in conventional group (P>0.05). During the 6-month follow-up, the number of acute attacks in the probiotic group (0.41±0.16) was significantly lower than that in the conventional group (0.87±0.23), and the difference was statistically significant (P<0.05). Conclusion: Intestinal probiotics in the adjuvant treatment of bronchial asthma can improve the immune function of children and reduce the number of acute attacks. The possible mechanism is related to improving the structure of intestinal flora and reducing the expression of EOS%, integrin α4β1 and αvβ6 in sputum. 2023 Vol. 29 (4): 595-600 [Abstract] ( 93 ) HTML (1 KB)  PDF (1253 KB)  ( 182 ) 600 Unstable Support Surface Core Stability Training in Postoperative Rehabilitation of Thoracolumbar Compression Fractures in the Elderly LUO Jingjing, YANG Fengjie, ZHENG Juan, et al DOI: 10.3969/j.issn.1006-6233.2023.04.015 Objective: To explore the application value of unstable support surface core stabilization training in postoperative rehabilitation of thoracolumbar compression fractures in the elderly. Methods: One hundred and five elderly postoperative patients with thoracolumbar compression fractures treated in our hospital from January 2019 to February 2022 were selected, and the patients were divided into observation group (n=52) and control group (n=53) using simple random sampling method, and the observation group was given core stabilization training on unstable support surfaces, and the control group was given conventional rehabilitation interventions, and the two groups were observed before and after interventions of Japanese Orthopaedic Association (Japanese The Japanese Orthopaedic Association (JOA) thoracolumbar spine score, Barthel Index (BI) score, gait and static balance parameters, and complications were observed before and after the intervention. Results: After the intervention, the JOA score and BI score of the observation group were (24.40 ± 1.41) and (58.21 ± 5.03) respectively, which were significantly higher than those of the control group (20.02±1.28)and(49.94±5.88)(P<0.05). After the intervention, the stride length, stride frequency and comfortable stride speed of the observation group were (0.56 ± 0.16) m, (1.08 ± 0.26) steps / s and (0.34 ± 0.10) m / s respectively, which were significantly higher than those of the control group(0.50±0.13)m,(0.98±0.22)steps / s,(0.25±0.11)m/s (P<0.05). In the observation group, the trajectory length of closed eye, trajectory length per unit time of closed eye, deviation of center of gravity of open eye X-axis movement, deviation of center of gravity of closed eye X-axis movement, deviation of center of gravity of open eye Y-axis movement, and deviation of center of gravity of closed eye Y-axis movement were (1860.60±335.54) mm, (32.21±5.83) mm, (11.50±2.12) mm, ( (11.89±3.65) mm, (12.12±1.99) mm and (15.10±3.31) mm, which were significantly lower than the control group (2100.10±365.12) mm, (36.65±6.04) mm, (15.59±1.92) mm, (20.10±5.01) mm, (16.60±2.03) mm and (19.14±3.01) mm, respectively. (19.14±3.01)mm (p<0.05). Conclusion: Unstable support surface core stability training has good application value in postoperative rehabilitation of thoracolumbar compression fractures in the elderly, which helps patients recover the function of thoracolumbar spine, improve postural control and daily living activities. 2023 Vol. 29 (4): 600-605 [Abstract] ( 55 ) HTML (1 KB)  PDF (1248 KB)  ( 118 ) 605 Expression of miR-155 and miR-143 in Patients with Premature Ovarian Insufficiency and Their Predictive Valne on Successful Pregnancy HAO Dongmei, YU Yuexin, ZHAO Yingying DOI: 10.3969/j.issn.1006-6233.2023.04.016 Objective: To analyze the expression of microRNA (miR)-155 and miR-143 in patients with premature ovarian insufficiency (POF), and to explore their predictive effect on successful pregnancy. Methods: A total of 172 patients with POF in our hospital from June 2020 to December 2021 were selected as the research objects, and 86 healthy people without POF during the same period were selected as the control. The expressions of miR-155 and miR-143 were compared between POF patients and non-POF healthy subjects, and the pregnancy was counted. Logistic regression was used to analyze the influencing factors of successful pregnancy in POF patients. The value of successful pregnancy in patients was analyzed with POF assessed by miR-155 and miR-143 by subject operating characteristic curve (ROC), decision curves were plotted to analyze the benefit of successful pregnancy in patients with POF assessed by miR-155 and miR-143, and the rate of successful pregnancy in patients with POF was compared with different expression of miR-155 and miR-143. Results: Patients with POF had lower miR-155 expression levels and higher miR-143 expression levels than healthy subjects with non-POF (P<0.05). There were 79 successful pregnancies and 93 non-pregnant cases in 172 POF patients; compared with non-pregnant POF patients, those with successful pregnancies had higher basal follicle counts, shorter duration of infertility, younger female age, younger male age, higher expression levels of anti-müllerian hormone and miR-155, and lower expression levels of miR-143 (P<0.05). Basal follicle count of 2-4 and high miR-155 expression were independent protective factors for pregnancy failure in POF patients, and low miR-143 expression was an independent risk factor for pregnancy failure in POF patients (P<0.05). The AUC value of the combined miR-155 and miR-143 assay to assess successful pregnancy in patients with POF was 0.812 (95% CI: 0.746 to 0.867), which was higher than the AUC values of miR-155 (χ2=2.625, P=0.009) and miR-143 (χ2=2.973, P=0.003) alone. Decision curve analysis showed that the net benefit of the combined miR-155 and miR-143 test in assessing successful pregnancy in POF patients was higher than that of the test alone when the risk threshold was 0.10 to 0.63. Decision curve analysis showed that the net benefit of the combined miR-155 and miR-143 test in assessing successful pregnancy in POF patients was higher than that of the test alone when the risk threshold was 0.10 to 0.63. miR-155+/miR-143- had a significantly higher rate of successful pregnancy than miR-155+/miR-143+, miR 155-/miR-143- and miR-155-/miR-143+ (P<0.05). Conclusion: POF patients with low miR-155 expression and high miR-143 expression, both of which have a direct impact on the success of pregnancy in POF patients. miR-155 high expression and miR-143 low expression have a higher success rate of pregnancy in POF patients, and the detection of miR-155 and miR-143 can predict pregnancy, which has certain clinical application value. 2023 Vol. 29 (4): 605-612 [Abstract] ( 46 ) HTML (1 KB)  PDF (1719 KB)  ( 83 ) 612 Effect of Metformin on Reducing the Incidence of Cardiovascular Events and Coronary In-Stent Restenosis after Coronary Intervention in Patients with Coronary Heart Disease FANG Yong, LU Wei, YAN Yu DOI: 10.3969/j.issn.1006-6233.2023.04.017 Objective: To investigate the effect of metformin on the incidence of in-stent restenosis and cardiovascular events in patients with coronary heart disease who underwent coronary intervention. Methods: From February 2020 to February 2022, 50 patients with coronary heart disease who received coronary intervention in Wanbei Coal and Electricity Group General Hospital were selected retrospectively. According to the postoperative treatment methods, they were divided into two groups: metformin group (metformin plus conventional treatment) and conventional treatment group (25 patients in each group). The general information, laboratory indicators, cardiovascular events and in stent restenosis of the two groups were comparatively analyzed. Results: After treatment, the levels of blood glucose, insulin resistance index, low density lipoprotein, total cholesterol, endolipin, tumor necrosis factor-αand hypersensitive C-reactive protein in 2 groups were lower in the model than before treatment (P<0.05), while the levels of omentin-1 in the model were higher than before treatment (P<0.05). The levels of blood glucose, insulin resistance index, low density lipoprotein, total cholesterol, endolipin, tumor necrosis factor-αand hypersensitive C-reactive protein in metformin group were significantly lower than those in conventional treatment group (P<0.05), while the omentin-1 level model was higher than that in conventional treatment group (P<0.05). The incidence of cardiovascular events in metformin group was 8.00% (2/25) lower than that in conventional treatment group 32.00% (8/25) (χ2=4.500, P<0.05). The incidence of coronary restenosis in metformin group was 12.00% (3/25) significantly lower than that in conventional treatment group (36.00% (9/25) (χ2=3.947, P<0.05). Conclusions: The addition of metformin to conventional treatment in patients with coronary heart disease undergoing coronary intervention can further reduce the levels of blood glucose, blood lipid, inflammatory factors and fat factors, and reduce the incidence of coronary stent restenosis and cardiovascular events. 2023 Vol. 29 (4): 612-616 [Abstract] ( 61 ) HTML (1 KB)  PDF (1222 KB)  ( 102 ) 617 Distribution of Pathogenic Bacteria, Drug Resistance and Factors Influencing Postoperative Infection in Patients with Osteoarthritis of the Knee Joint CHAI Jing, WANG Doudou, WANG Jiao, et al DOI: 10.3969/j.issn.1006-6233.2023.04.018 Objective: To investigate the distribution of pathogenic bacteria, drug resistance and influencing factors of postoperative pseudojoint infection in patients with knee osteoarthritis in Shijiazhuang Third Hospital from 2016 to 2020. Methods: Totally 1509 patients with knee osteoarthritis who underwent knee surgery in Shijiazhuang Third Hospital from January 2016 to January 2020 were selected and divided into infection group (38 cases) and non-infection group (1471 cases) according to postoperative infection. The knee joint effusion of the infected patients was collected and cultured to detect the types of pathogenic bacteria and drug resistance. Clinical data were collected and multivariate Logistic regression was used to analyze the factors influencing postoperative infection in patients with knee osteoarthritis. Results: A total of 46 strains of pathogenic bacteria were detected, of which 86.96% were Gram-positive bacteria, Gram-positive bacteria were resistant to β-lactam, quinolones and other antibiotics.Duration of operation ≥3h, type 2 diabetes, rheumatoid arthritis, smoking, postoperative urinary tract infection and prevention without antibiotics were risk factors for postoperative periprosthetic infection in patients with knee osteoarthritis (P<0.05). Conclusion: Gram-positive bacteria are the main pathogenic bacteria of pseudojoint infection after knee osteoarthritis, and have high drug resistance rate. Long operation time, type 2 diabetes mellitus, rheumatoid arthritis, smoking, postoperative urinary tract infection and failure to use antibiotics to prevent infection are the main factors leading to infection around pseudarthrosis. Infection monitoring of high-risk patients should be strengthened and antibiotics should be rationally used to reduce the risk of infection. 2023 Vol. 29 (4): 617-622 [Abstract] ( 68 ) HTML (1 KB)  PDF (1229 KB)  ( 106 ) 622 Study on the Effectiveness of Eyemax Insight Pancreaticobiliary Imaging System for Endoscopic Retrograde Cholangiopancreatography in the Treatment of Common Bile Duct Stones LI Changzhou, HU Xue, GUO Tianyu, et al DOI: 10.3969/j.issn.1006-6233.2023.04.019 Objective: To investigate the application effect of eyemax Insight pancreaticobiliary imaging system in endoscopic retrograde cholangiopancreatography (ERCP) in the treatment of common bile duct stones. Methods: A total of 80 patients who received common bile duct surgery in our hospital from January 2021 to August 2022 were recruited. After routine ERCP lithotomy, eyemax Insight pancreatic biliary imaging system was used to observe the residual stones of common bile duct under endoscopic retrograde cholangiopancreatography, and nasobiliary drainage tube was placed. The safety and efficacy of this treatment were analyzed. Results: In this study, 80 patients with common bile duct stones were successfully treated with endoscopic retrograde cholangiopancreatography by eyemax Insight pancreatic biliary imaging system. The average time of the insight pancreaticobiliary imaging was 23.0±3.2min, and the tatal operation time was 64.0±5.4min. Three days after operation, the nasobiliary duct was performed for choledochography, and there were no residual stones in the bile duct. Three days after operation, the biochemical indexes including Tbil, TBA, Dbil, and IBil among 80 patients were significantly improved compared to those before operation, and the statistical analysis showed statistical significance (P<0.05). All patients recovered successfully and were discharged from hospital. Conclusion: Eyemax Insight pancreatobiliary imaging system has high safety performance in endoscopic retrograde cholangiopancreatography for the treatment of common bile duct stones, which provides a favorable way for clinical treatment, and is worthy of promotion and application. 2023 Vol. 29 (4): 622-625 [Abstract] ( 99 ) HTML (1 KB)  PDF (1228 KB)  ( 598 ) 626 Clinical Effects of Transcatheter Trabeculectomy Combined with Intravitreal Injection of Ranibizumab for Neovascular Glaucoma and the Effects on Visual Acuity and Intraocular Pressure GUO Bin, LI Jinjin DOI: 10.3969/j.issn.1006-6233.2023.04.020 Objective: To study the effect of intravitreal injection of ranibizumab therapy in combination with trans-trabeculectomy in the treatment of neovascular glaucoma (NVG). Methods: By retrospective analysis, 70 NVG patients admitted from January 2020 to January 2022 were enrolled and divided into experimental group (35 cases) and control group (35 cases) according to different treatment methods. The experimental group received intravitreal injection of ranibizumab combined with trans-trabeculectomy, while the control group received trans-trabeculectomy. The incidence of IOP levels, visual acuity levels, ocular arterial hemodynamic parameters [coefficient of resistance (RI), peak systolic flow velocity (PSV), end-diastolic flow velocity (EDV)], serum interleukin-6 (IL-6) and overall efficiency after treatment, complications (ocular pain, anterior chamber blood accumulation, hypotension, corneal edema) were compared between the two groups before treatment and after 3 months of treatment. Results: Before treatment, there were no significant differences in intraocular pressure, visual acuity, RI, PSV, EDV and IL-6 levels between the two groups (P>0.05). After treatment, the levels of visual acuity, PSV and EDV in 2 groups were higher than before, while the levels of intraocular pressure, RI and IL-6 were lower than before, with statistical significance (P<0.05). The differences of visual acuity, PSV, EDV, Intraocular pressure, RI and IL-6 before and after treatment were statistically significant between the two groups (P<0.05). The total effective rate of the experimental group was higher than that of the control group, and the complication rate was lower, the difference was statistically significant (P<0.05). Conclusion: Intravitreal injection of ranibizumab combined with trans-trabeculectomy has significant effect in NVG treatment, which can effectively improve visual acuity, reduce intraocular pressure, improve arterial hemodynamics and IL-6 levels, and reduce complications. 2023 Vol. 29 (4): 626-630 [Abstract] ( 64 ) HTML (1 KB)  PDF (1229 KB)  ( 186 ) 630 Characteristics of CRP VCAM-1 NHR Lipid Metabolism and Their Correlation with Cardiac Insufficiency in Patients with H-Type Hypertension Complicated with Coronary Artery Disease JIN Yuxia, MA Li, LI Qingqing, et al DOI: 10.3969/j.issn.1006-6233.2023.04.021 Objective: To investigate the correlation between C-reactive protein (CRP), vascular endothelial adhesion molecule-1 (VCAM-1), neutrophil/HDL cholesterol ratio (NHR), lipid metabolism characteristics and with cardiac insufficiency in patients with H-type hypertension with coronary artery disease. Methods: 104 patients with hypertension complicated with coronary artery disease treated in our hospital from January 2020 to January 2022 were selected, including 49 patients with H-type hypertension complicated with coronary artery disease (observation group) and 55 patients with non-H-type hypertension complicated with coronary artery disease (control group), the serum CRP, VCAM-1, NHR, lipid metabolism indexes and cardiac function grades of the two groups were compared, and the differences in relevant indexes of patients with different cardiac functional grades were analyzed at the same time. Results: Compared with the control group, serum CRP, VCAM-1 and NHR of the observation group were higher [(5.15±1.03) mg/L, (780.05±81.16) ng/ml and (5.05±0.79) ng/ml)](P<0.05), while the high-density lipoprotein cholesterol (HDL-C) and apolipoprotein AI (APOAI) were lower [(1.03 ± 0.24) mmol/l and (0.66±0.29) g/L](P<0.05). Compared with the control group, the NYHA grade III ~ IV ratio, left ventricular end diastolic diameter (LVDD), left atrial diameter (LAD) and Gensini score in the observation group were higher [61.22%, (4.66±0.40) cm, (4.66±0.40) cm, (4.01±0.39) cm and (52.26±13.35) points](P<0.05), while the left ventricular ejection fraction (LVEF) was lower [(52.64±5.16)%](P<0.05). The scores of CRP, VCAM-1, NHR and Gensini in patients with grade III ~ IV cardiac function in the observation group were (5.32±0.72) mg/L, (804.69 ± 69.50) ng/ml, (5.30±0.90) and (55.15±9.31) respectively, which were significantly higher than those in patients with grade I ~ II (P<0.05), while HDL-C and LVEF were (0.97±0.23) mmol/l and (51.52±4.60)% respectively, which were significantly lower than those in patients with grade I ~ II (P<0.05). NYHA grade was positively correlated with CRP, VCAM-1 and NHR (P<0.05), but negatively correlated with HDL-C (P<0.05). Gensini score was positively correlated with NHR (P<0.05), but negatively correlated with HDL-C (P<0.05). Conclusion: The levels of CRP, VCAM-1 and NHR in patients with H-type hypertension complicated with coronary artery disease increase, the three factors are positively correlated with cardiac function grade and coronary severity. 2023 Vol. 29 (4): 630-636 [Abstract] ( 75 ) HTML (1 KB)  PDF (1300 KB)  ( 63 ) 636 Clinical Efficacy of Ultrasonic Guided Closed Reduction K-Wires Transverse Internal Fixation for Metacarpal Fracture LI Yali, et al DOI: 10.3969/j.issn.1006-6233.2023.04.022 Objective: To investigate the clinical efficacy of ultrasonic guided closed reduction K-wires transverse internal fixation for metacarpal fracture. Methods: The data of 84 patients with metacarpal fractures treated in our hospital from March 2017 to March 2020 were retrospectively analyzed. The observation group of 36 patients were treated with closed reduction K-wires transverse internal fixation under ultrasound guidance,and active flexion and extension of fingers began on the first postoperative day.X-ray was re-examined 4-6 weeks after surgery and the K-wires were removed. In the control group, 48 patients received internal fixation with mini-plate, active flexion and extension functional exercise of fingers began on the first postoperative day, and X-ray was reviewed at 1st, 2nd, 3rd, 6th months. Results: Patients operated on the fracture of metacarpal bones were followed up for 12-18 months, with an average of 14.6 months. There were no serious complications such as infection, malformation or delay in healing. The observation group had significant advantages over the control group in operation time, intraoperative blood loss, fracture healing time and hospital stay (P<0.05). Total motion of flexion and extension (TAM) was used to assess hand function. The excellent and good rate was 100% in observation group. The excellent and good rate of the control group was also 100%. There was no significant difference between the two groups (P>0.05). Conclusion: Ultrasonic guided closed reduction K-wires transverse fixation in the treatment of metacarpal fracture is simple and easy to operate, with less radiation, less trauma, less bleeding, short operation time, and fast fracture healing, especially for pregnant women and teenagers, and it is worthy of clinical promotion. 2023 Vol. 29 (4): 636-641 [Abstract] ( 99 ) HTML (1 KB)  PDF (2792 KB)  ( 219 ) 641 Follow-Up Observation of Vaginal Microenvironment after LEEP and Analysis of Risk Factors for Persistent HPV Infection WANG Xiaoli, YAN Yifen DOI: 10.3969/j.issn.1006-6233.2023.04.023 Objective: To investigate the follow-up observation of vaginal microenvironment after loop electrosurgical excision procedure (LEEP) and the influencing factors of persistent human papillomavirus (HPV) infection. Methods: From January 2019 to September 2021, 98 patients with LEEP in our hospital were selected, according to the return visit of one year after LEEP, 20 patients with persistent HPV infection,the differences of clinical data between persistent HPV infection and HPV negative patients were analyzed, and the differences of vaginal microenvironment before and 1 week after LEEP were analyzed, and the differences of vaginal microenvironment between patients with persistent HPV infection and those with negative HPV during follow-up visit were also analyzed. Results: Compared with negative HPV patients, the age ≥ 40 years old, vaginal wall involvement, multiple infection of HPV before operation, HPV viral load ≥ 100 RLU/CO before operation, and positive rate of incisional marginof patients with persistent HPV infection were 80.00%, 65.00%, 60.00%, 65.00% and 65.00% respectively, which were significantly higher (P<0.05). Compared with negative HPV patients, the rates of condom use and anti HPV drugs use in patients with persistent HPV infection were 40.00% and 35.00%, respectively, which were significantly lower (P<0.05). Logistic regression showed that age, multiple infection of HPV before operation, load of HPV virus before operation, incision margin, and use of anti HPV after operation were the influencing factors of persistent infection of HPV (P＜0.05). After LEEP, the proportion of colony density Ⅲ～Ⅳ, flora diversity Ⅲ～Ⅳ, pH>4.5, salivary glycosidase positive, hydrogen peroxide positive, leukocyte esterase positive were 21.43%, 26.53%, 32.65%, 37.76%, 31.63% and 25.51% respectively, which were significantly lower than those before LEEP (P<0.05). Compared with negative HPV patients, the proportion of vaginal microenvironment flora density III-IV, flora diversity III-IV, pH>4.5, salivary glycosidase positive, hydrogen peroxide positive, and leukocyte esterase positiveduring follow-up visit of persistent HPV infection were 60.00%, 55.00%, 60.00%, 55.00%, 50.00%, and 60.00% which were significantly higher (P<0.05). Conclusion: The persistent infection of HPV is affected by the patient's age and multiple HPV infections and so on. The vaginal microenvironment is significantly improved after LEEP, and the vaginal microenvironment of patients with persistent HPV infection at the time of follow-up visits were significantly worse than that of patients with negative HPV. 2023 Vol. 29 (4): 641-647 [Abstract] ( 99 ) HTML (1 KB)  PDF (1252 KB)  ( 169 ) 647 Efficacy of Three Root Canal Sealants Combined with Hot Gutta-Percha Vertical Compression Technique in the Treatment of Dental Pulp Disease WANG Xiaoying, WANG Yanhua, WANG Bianhong DOI: 10.3969/j.issn.1006-6233.2023.04.024 Objective: To investigate the efficacy of three root canal sealants (AH Plus, Gutta Flow, iRoot SP) combined with hot gutta-percha vertical compression technique in the treatment of dental pulp disease. Methods: One hundred and eighty patients (180 affected teeth) with dental pulp disease were selected and randomly divided into AH Plus group, Gutta Flow group and iRoot SP group by the random number grouping method, with 60 cases in each group. The three groups selected AH Plus, Gutta Flow and iRoot SP as root canal sealants respectively, and then they were combined with hot gutta-percha vertical compression technique for root canal filling treatment. The quality of root canal filling, filling time, filling area ratio, pain occurrence (Mohd score standard) after surgery, serum inflammatory factors levels [serum interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α)] one week before and after surgery and efficacy at 6 months after surgery were compared among the three groups. Results: The accurate filling rates of AH Plus group, Gutta Flow group and iRoot SP group were 96.67%, 98.33% and 98.33% respectively. There was no statistical significance in filling quality among the three groups (P>0.05). The filling time of Gutta Flow group and iRoot SP group was shorter than that of AH Plus group the differences has statiscal sighificance (P<0.0167). The pain rates of AH Plus group, Gutta Flow group and iRoot SP group at 1 week after surgery were 16.67%, 5.00% and 3.33% respectively, and there were statistical differences in pain grading and pain rate among the three groups (P<0.05), the pain rate was lower in Gutta Flow group and iRoot SP group than that in AH Plus group the differences has statisthcal sigificance (P<0.0167). The time-point effect, between-group effect and interaction effect of between-group and time-point of serum TNF-α and IL-6 revealed statistical differences among the three groups (P<0.05), the two levels of the three groups after treatment were lower than those before treatment (P<0.05), and the levels were lower in Gutta Flow group and iRoot SP group than AH plus group (P<0.0167) they all has statistical significance. The effective rates of AH Plus group, Gutta Flow group and iRoot SP group at 6 months after surgery were 81.67%, 90.00% and 96.67% respectively, and there were statistical differences in effective rates and efficacy level of the three groups (P<0.05), and the effective rate of iRoot SP group was higher than that of AH Plus group (P<0.0167). Conclusion: The apical sealing performance of iRoot SP or Gutta Flow as root canal sealants combined with hot gutta-percha vertical compression technique in the treatment of dental pulp disease is better than AH Plus, and the former two can shorten the filling time, relieve the postoperative pain and improve the inflammatory response, but the long-term apical sealing efficacy of iRoot SP is better than Gutta Flow. 2023 Vol. 29 (4): 647-652 [Abstract] ( 83 ) HTML (1 KB)  PDF (1259 KB)  ( 236 ) 652 Study on the Effects of Different Treatments on Heart Rate Variability Serum KLK1 and TIMP-1 Levels and Short-Term Prognosis in Patients with Coronary Artery Disease XIE Xiaoli, et al DOI: 10.3969/j.issn.1006-6233.2023.04.025 Objective: To investigate the effects of domestic rapamycin drug-eluting stent and paclitaxel drug-eluting balloon on heart rate variability, levels of serum Kallikrein 1 (KLK1) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) and short-term prognosis in patients with coronary heart disease. Methods: The data of 105 patients with coronary artery disease who received percutaneous coronary intervention in the hospital from November 2019 to May 2021 were reviewed, and they were divided into the observation group (56 cases, using domestic rapamycin drug-eluting stent) and the control group (49 cases, treated with domestic paclitaxel drug-eluting balloon) according to different stents used. The heart rate variability-related indicators [standard deviation of normal sinus RR interval within 5 min (SDNN), standard deviation of the 5-minute average RR intervals (SDANN), percent of differences between adjacent NN intervals greater than 50 ms (pNN50)] before surgery, at 1 week after surgery and at 1 year follow-up, levels of KLK1, TIMP-1, endothelin-1 (ET-1) and nitric oxide (NO) before surgery and at 1 week after surgery as well as short-term prognosis [vascular re-stenosis, vascular re-thrombosis, major adverse cardiovascular events (MACE)] at 1 year of follow-up were compared between the two groups. Results: There were statistical differences in the SDNN, SDANN and pNN50 in repeated measures analysis of variance between the two groups form the aspect of time-point effect (P<0.017), and the above three indicators showed upward trends with the prolongation of postoperative time. The absolute values of differences of serum KLK1, TIMP-1, NO and plasma ET-1 before and after surgery revealed statistically significant differences between the two groups (P<0.05), and the changes in the observation group were greater than those in the control group. At 1 year of follow-up, the incidence rate of vascular re-stenosis in the observation group was lower than that in the control group (P<0.05), but there were no statistically significant differences in the incidence rates of vascular re-thrombosis and MACE between the two groups (P>0.05). Conclusion: Domestic rapamycin drug-eluting stent can effectively regulate heart rate variability in patients with coronary artery disease, and it has a positive effect on improving serum KLK1 and TIMP-1 levels, and can help protect and repair the endothelial function, reduce the risk of stent stenosis, and achieve the good short-term prognosis. 2023 Vol. 29 (4): 652-657 [Abstract] ( 61 ) HTML (1 KB)  PDF (1258 KB)  ( 177 ) 658 Association of MPVLR Glasgow Prognostic Score and Prognosis within One Year of PCI in Patients with Acute Coronary Syndrome ZHANG Tongchang, LI Haishan DOI: 10.3969/j.issn.1006-6233.2023.04.026 Objective: To explore the correlation of the mean platelet volume to lymphocyte ratio (MPVLR), Glasgow prognosis score (Glasgow) and the prognosis of patients with acute coronary syndrome after percutaneous coronary intervention (PCI). Methods: A total of 98 patients with acute coronary syndrome treated in our hospital from January 2019 to July 2021 were selected, according to whether major adverse cardiovascular events (MACE) such as cardiac or all-cause death and acute heart failure occurred one year after operation, they were divided into poor prognosis group and good prognosis group, with 30 cases and 68 cases respectively. The differences of clinical data such as GRACE score, Glasgow score and MPVLR of patients with poor prognosis and those with good prognosis were observed and compared. Results: The admission GRACE score of patients with poor prognosis was (145.50 ± 19.29), which was significantly higher than that of patients with good prognosis (P<0.05). The proportion of Glasgow score 1-2 in patients with poor prognosis was 80.00%, which was significantly higher than that in patients with good prognosis (P<0.05). The peak values of MPVLR and cardiac troponin T (cTnT) in patients with poor prognosis were (6.90 ± 1.01) and (6.69 ± 1.32) ng / ml, respectively, which were significantly higher than those in patients with good prognosis (P<0.05). The left ventricular ejection fraction (LVEF) of patients with poor prognosis was (46.60 ± 7.82)%, which was significantly lower than that of patients with good prognosis (P<0.05); Logistic regression analysis showed that Glasgow score, MPVLR and LVEF were the influencing factors of poor prognosis after PCI in patients with acute coronary syndrome (P<0.05). Conclusion: MPVLR and Glasgow scores at admission are related to poor prognosis of patients with acute coronary syndrome after PCI, which is worthy of further study. 2023 Vol. 29 (4): 658-663 [Abstract] ( 63 ) HTML (1 KB)  PDF (1251 KB)  ( 104 ) 663 Effects of Ultrasound-Guided MMT Minimally Invasive Rotational Resection on Perioperative Indicators Postoperative Pain Scarring and Complications in Women with Benign Breast Masses ZHOU Ying, LIU Tao DOI: 10.3969/j.issn.1006-6233.2023.04.027 Objective: To explore the effects of ultrasound-guided MMT minimally invasive rotational resection on perioperative indicators, postoperative pain, scarring and complications of benign breast masses in women. Methods: The clinical data of 126 patients with benign breast masses who were treated in the hospital from January 2019 to January 2022 were retrospectively analyzed. According to the different surgical methods, the patients were divided into MMT group and conventional group. The 71 cases in MMT group underwent ultrasound-guided MMT minimally invasive rotational resection, and the 55 cases in conventional group were treated with traditional open surgery. The perioperative indicators (surgical time, intraoperative blood loss, incision length, incision healing time) and postoperative pain [Visual Analogue Scale (VAS) score at 2, 8 and 24 hours after surgery] were compared between the two groups. The patients were followed up for 3 months after surgery, and the scars [scar length, Vancouver Scar Scale (VSS)] and differences in occurrence of complications were compared. Results: The surgical time, intraoperative blood loss, incision length, incision healing time, VAS scores at various postoperative time periods, various indicators of scar conditions and total incidence rate of complications were all shorter or lower in MMT group compared to conventional group (P<0.05). Conclusion: Ultrasound-guided MMT minimally invasive rotational resection for female patients with benign breast masses compared with conventional surgery can enhance the surgical efficiency, reduce the surgical trauma and shorten the postoperative recovery process, improve the postoperative pain, and reduce the scar formation and risk of complications. 2023 Vol. 29 (4): 663-667 [Abstract] ( 93 ) HTML (1 KB)  PDF (1256 KB)  ( 172 ) 667 Study of the Relationship between Vitamin D and Microinflammation and Falls in Patients Treated with Hemodialysis MAO Hui, ZHANG Li, ZHAO Qian, et al DOI: 10.3969/j.issn.1006-6233.2023.04.028 Objective: To analyze the relationship between 25 hydroxyvitamin D, microinflammation and fall in patients treated with hemodialysis. Methods: A total of 80 patients receiving maintenance hemodialysis (MHD) treatment in Chengde Central Hospital from January 2020 to June 2020 were divided into 25(OH)D low group and normal group according to their 25(OH)D level. Gender, age, dialysis age, height, weight, primary disease ,blood purification method of the patients and the occurrence of falls in the past one year were recorded. Blood procalcitonin (PCT), haemoglobin (Hb), blood albumin, blood calcium, blood phosphorus, parathyroid hormone, triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol and other indicators were tested. The relationship between 25(OH)D, PCT and fall was analyzed by Pearson correlation analysis,multiple linear regression and binary Logistic analysis. Results: The level of PCT, TG and TC in 25 (OH) D normal group were lower than those in 25 (OH) D low group, and the differences were statistically significant (P<0.05). The level of Hb in the 25 (OH) D normal group was higher than that in the 25(OH)D low group, and the difference was statistically significant (P<0.05). Pearson correlation analysis showed that there was a negative correlation between PCT,TC and 25(OH)D level. Multivariate regression analysis showed that PCT and TC were independent influencing factors of 25(OH)D level, and 25(OH)D level was an protective factor affecting fall in MHD patients. Conclusion: PCT plays an important role in the 25(OH)D level of MHD patients, it is an independent risk factor affecting 25(OH)D level,clinical attention should be paid to the correction of PCT level in MHD patients. Active vitamin D supplementation is also needed to improve 25(OH)D levels, reduce the incidence of falls, and continuously improve the quality of survival. 2023 Vol. 29 (4): 667-672 [Abstract] ( 64 ) HTML (1 KB)  PDF (1253 KB)  ( 159 ) 672 Effect of Nimesulide Combined with Loxofen Sodium on TIMP-1 MMP-3 and Bone Metabolic Indexes in Patients with Knee Osteoarthritis LI Bo, BAI Ningjuan DOI: 10.3969/j.issn.1006-6233.2023.04.029 Objective: To investigate the effects of nimesulide combined with loxofen sodium on TIMP-1, MMP-3 and bone metabolism in patients with knee osteoarthritis. Methods: From January 2019 to September 2021, 92 patients with knee osteoarthritis were included in this study as the observation subjects and divided into control group (n=46) and combined group (n=46) according to the randomized-control principle. The control group was given loxofen sodium. The combined group was given nimesulide combined with loxofen sodium both group for 4 weeks as a course of treatment. All patients completed the treatment and were followed up. The efficacy of the two groups was evaluated at the end of the 4-week course of treatment. The serum levels of matrix metalloproteinase 3 (MMP-3), matrix metalloproteinase inhibitor 1 (TIMP-1), osteocalcin (BGP) and osteoprotegerin (OPG) were measured before and after treatment, and the adverse reactions were recorded. Results: The total effective rate of the control group was 82.61% (38/46), which was lower than that of observation group 95.65% (44/46), and the difference was statistically significant (P<0.05). Compared with before treatment, serum MMP-3 decreased and serum TIMP-1 increased in the two groups after 4 weeks of treatment, the difference of serum MMP-3 and TIMP-1 before and after treatment was statistically significant (P<0.05). Compared with before treatment,the serum BGP and OPG levels increased in both groups after 4 weeks of treatment. The difference of serum BGP and OPG levels before and after treatment between the two groups was statistically significant (P<0.05).There was no statistical significance in the adverse reaction rate between the two groups (P>0.05). Conclusion: Nimesulide combined with loxofen sodium can improve the treatment efficacy among knee osteoarthritis patients, improve bone metabolism and matrix metalloprotein level with high safety. 2023 Vol. 29 (4): 672-676 [Abstract] ( 118 ) HTML (1 KB)  PDF (1243 KB)  ( 93 ) 677 Clinical Efficacy of Phentolamine Pump Maintenance in the Treatment of Neonatal Necrotizing Enterocolitis HU Penglin, CHANG Kaichao, DENG Shengmin, et al DOI: 10.3969/j.issn.1006-6233.2023.04.030 Objective: To study the clinical efficacy of phentolamine pump maintenance in the treatment of neonatal necrotizing enterocolitis (NEC). Methods: A total of 96 children with NEC admitted to Kunming Children's Hospital of Yunnan Province from January 2019 to January 2022 were enrolled in this retrospective analysis. According to different treatment methods, they were divided into study group (48 cases) and control group (48 cases). The control group received conventional treatment, and the study group received phentolamine pump maintenance treatment based on conventional therapy. Comparison was made of serum inflammatory factors [tumor necrosis factor-α (TNF-α), C-reactive protein (CRP), interleukin-10 (IL-10)], immune indexes [complement 3 (C3), complement 4 (C4), immunoglobulin M (IgM), immunoglobulin A (IgA)] levels and time to resume enteral nutrition, improvement time of stool, disappearance time of abdominal distension, and total effective rate before and after treatment in the two groups. Results: Before treatment, there were no significant differences in the levels of TNF-α, CRP, IL-10, C3, C4, IgM and IgA between the two groups (P>0.05). After treatment, the levels of TNF-α, CRP and IL-10 in the two groups were decreased compared with those before treatment, while the levels of C3, C4, IgM and IgA were increased compared with those before treatment, and the differences were statistically significant (P<0.05). The differences of TNF-α, CRP,IL-10 , C3, C4, IgM and IgA before and after treatment were statistically significant between the two groups (P<0.05). The recovery time of intestinal nutrition, improvement time of stool and disappearance time of abdominal distension were shorter in the study group, and the total effective rate was higher than that in the control group, the difference was statistically significant (P<0.05). Conclusion: Phentolamine pump is effective in the maintenance treatment of NEC, which can reduce the inflammatory response and improve the gastrointestinal and immune function. 2023 Vol. 29 (4): 677-681 [Abstract] ( 76 ) HTML (1 KB)  PDF (1240 KB)  ( 97 ) 681 Curative Effect of Budesonide Nebulization Combined with Ambroxol in the Treatment of Pediatric Bronchopneumonia and Its Effect on Serum CRP and PCT Levels LIU Le, WANG Junxia, GAN Xinru, et al DOI: 10.3969/j.issn.1006-6233.2023.04.031 Objective: To investigate the curative effect of budesonide nebulization combined with ambroxol in the treatment of pediatric bronchopneumonia and its effect on serum CRP and PCT levels. Methods: A total of 186 children with bronchopneumonia treated in our hospital between June 2020 to September 2021 were enrolled and divided into observation group (n=93) and control group (n=93) by random number table method. The control group was treated with budesonide nebulization, while observation group was additionally treated with intravenous drip of ambroxol. The clinical curative effect after treatment, levels of inflammatory factors (CRP, PCT) and immune function (IgG, IgM, IgA) before and after treatment between the groups were compared. Results: After treatment, total response rate of observation group was higher than that of control group (93.55% vs 71.72%, P<0.05). After treatment, disappearance time of clinical symptoms was shorter in observation group than control group (P<0.05). After 5d of treatment, levels of CRP and PCT in both groups were decreased. The difference values of CRP and PCT before and after treatment in observation group were (25.93±4.54) mg/L and (1.03±0.25) μg/L, higher than those in control group [(16.15±3.72) mg/L, (0.84±0.19) μg/L, P<0.05]. After 5d of treatment, levels of IgA, IgM and IgG in both groups were increased. The difference values of IgA, IgM and IgG before and after treatment in observation group were (4.15±0.87) g/L, (0.46±0.13) g/L and (0.63±0.22) g/L, higher than those in control group [(1.67±0.39) g/L, (0.27±0.06) g/L, (0.27±0.08) g/L, P<0.05]. The incidence of adverse reactions between observation group and control group was similar the difference was not statistically significane [9.68% (9 cases) vs 7.53% (7 cases), P>0.05]. Conclusion: Compared with budesonide nebulization alone, curative effect of budesonide nebulization combined with ambroxol is better on children with bronchopneumonia, which can more effectively improve clinical symptoms, inflammatory factors and immune function, with certain safety. 2023 Vol. 29 (4): 681-685 [Abstract] ( 75 ) HTML (1 KB)  PDF (1250 KB)  ( 152 ) 685 Effects of Sequential Administration of Moxifloxacin and Levofloxacin on Respiratory Function and Microinflammation in Elderly Patients with Severe AECOPD WANG Chao, et al DOI: 10.3969/j.issn.1006-6233.2023.04.032 Objective: To study the effects of sequential administration of moxifloxacin and levofloxacin on respiratory function and microinflammation in elderly patients with severe acute exacerbation of chronic obstructive pulmonary disease (AECOPD). Methods: 150 elderly patients with severe AECOPD treated in our hospital from March 2019 to March 2022 were selected and divided into group A, B and C by simple random sampling method, with 50 cases in each group. Group A was given moxifloxacin, group B was given levofloxacin, and group C was given moxifloxacin and levofloxacin administered sequentially. The respiratory function indicators [forced expiratory volume in the first second as a percentage of the predicted value (fev1%pred) and forced expiratory volume in the first second (FEV1)/forced vital capacity (FVC)] were compared among the three groups after 1 week of treatment, and the changes of serum microinflammation [intercellular cell adhesion molecule (ICAM)-1, interferon gamma-inducible protein-10 (IP-10)] and immune function [plasma-soluble triggering receptor expressed on myeloid cells-1 (sTREM-1), peripheral blood CD4+/CD8+] were compared and analyzed before and after treatment. The differences in occurrence of adverse drug reactions among the three groups were compared. Results: After 1 week of treatment, fev1%pred, FEV1/FVC and peripheral blood CD4+/CD8+ were increased while serum ICAM-1, IP-10 and plasma sTREM-1 were decreased in the three groups the difference was statistically significant (P<0.05). Compared at the soone time the fev1%pred, FEV1/FVC and peripheral blood CD4+/CD8+ in group A were higher while serum ICAM-1, IP-10 and plasma sTREM-1 were lower than those in group B, and fev1%pred, FEV1/FVC and peripheral blood CD4+/CD8+ were higher while serum ICAM-1, IP-10 and plasma sTREM-1 were lower in group C compared to groups A and B the difference was statistically significant (P<0.05). The incidence rate of phlebitis in group C was lower than that in groups A and B the difference was statistically significant (P<0.05). There were no statistical differences in the incidence rates of other adverse reactions (P>0.05). Conclusion: Sequential administration of moxifloxacin and levofloxacin can alleviate the microinflammatory response in patients with AECOPD, improve the pulmonary function and immune indicators, and effectively prevent the occurrence of phlebitis. 2023 Vol. 29 (4): 685-689 [Abstract] ( 50 ) HTML (1 KB)  PDF (1246 KB)  ( 122 )

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