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2023 Vol. 29, No. 2 Published: 2023-02-28 177 LINC00342 Regulates the Effect of miR-384 on the Proliferation Invasion and Migration of Lung Squamous Carcinoma Cells XU Yueliang, WANG Xiaobin, YANG Yaoqing DOI: 10.3969/j.issn.1006-6233.2023.02.01 Objective: To investigate the influences of LINC00342 on the proliferation, invasion, and migration of lung squamous cell carcinoma through miR-384. Methods: Lung squamous cell carcinoma SK-MES-1 cells were grouped into the ctrl group (normally cultured SK-MES-1 cells), si-NC group (transfected with si-NC), and si-LINC00342 group (transfected with si-LINC00342), si-LINC00342+inhibitor-NC group (co-transfected with si-LINC00342 and inhibitor-NC), and si-LINC00342+ miR-384 inhibitor group (co-transfected with si-LINC00342 and miR-384 inhibitor); RT-qPCR was applied to detect the expression of LINC00342 and miR-384 in cells; CCK-8 assay and Transwell chamber were applied to detect cell proliferation and invasion; scratch assay and flow cytometry were used to cell migration and apoptosis; Western blot was applied to detect the expression of proliferating cell nuclear antigen (PCNA), matrix metalloproteinase-2 (MMP-2), MMP-9, and cysteine protease-3 (caspase-3) in cells; and dual-luciferase reporter assay was used to verify the relationship between LINC00342 and miR-384. Results: Compared with the ctrl group and si-NC group, the OD450 value, the number of cell invasions, the wound healing rate, the expressions of PCNA, MMP-2, and MMP-9 in SK-MES-1 cells in the si-LINC00342 group was obviously decreased, and the cell apoptosis was obviously decreased, the mortality rate and the expression of caspase-3 were obviously increased (P<0.05); inhibiting the expression of miR-384 attenuated the inhibitory effect of knockdown of LINC00342 on the proliferation, invasion, and migration of SK-MES-1 cells, and reduced the apoptosis ability; LINC00342 targeted miR-384 expression. Conclusion: Knockdown of LINC00342 may inhibit the proliferation, invasion, and migration of SK-MES-1 cells by targeting miR-384. 2023 Vol. 29 (2): 177-182 [Abstract] ( 268 ) HTML (1 KB)  PDF (2158 KB)  ( 580 ) 183 The Effect of LncRNA KCNQ1OT1 on the Malignant Development of Colorectal Cancer SW480 Cells by Regulating the miR-145/PAK4 Axis SHI Wen, ZHOU Zhiqiang, CAI Ming, et al DOI: 10.3969/j.issn.1006-6233.2023.02.02 Objective: To explore the role of LncRNA KCNQ1OT1 in colorectal cancer SW480 cells and its potential molecular mechanism. Methods: MiRanda and TargetScan were conducted to analyze the action targets of LncRNA KCNQ1OT1 and miR-145 respectively. The intermolecular target relationship was verified by dual luciferase reporter assay. The qRT-PCR assay was used to analyze the gene expression levels of KCNQ1OT1, miR-145, and PAK4 in colorectal cancer tissues, para cancer tissues, SW480 cells, and CCC-HIE-2 cells.SW480 cells were divided into siRNA NC group, KCNQ1OT1 siRNA group, PAK4 siRNA group, inhibitor NC group, miR-145 inhibitor group, pcDNA-3.1(+)+mimics NC group, pcDNA-KCNQ1OT1+mimics NC group, pcDNA-3.1(+)+miR-145 mimics group, pcDNA-KCNQ1OT1+miR-145 mimics group, the expressions of KCNQ1OT1, miR-145 and PAK4 were knocked down, respectively. Cell viability was analyzed by MTT assay, the number of clones was analyzed by cell colony formation assay, cell apoptosis rate was detected by flow cytometry, and the EMT ability of SW480 cells was detected by Western blot. Result: Compared with para cancer tissues (1.37±0.27), LncRNA KCNQ1OT1 expression in colorectal cancer tissues was up-regulated (2.32±0.89) (t=23.84, P<0.01). The expression of PAK4 in colorectal cancer tissues was up-regulated (2.03±0.54) compared with paracellular tissues (1.21±0.09) (t=20.75, P<0.01). Compared with para cancer tissues (1.26±0.35), the expression of miR-145 in colorectal cancer tissues was significantly down-regulated (0.45±0.13) (t=18.76, P<0.01). Compared with CCC-HIE-2 cells (1.05±0.21), LncRNA KCNQ1OT1 expression in SW480 cells was up-regulated (2.09±0.27) (t=18.79, P<0.01). Compared with CCC-HIE-2 cells (1.18±0.62), PAK4 expression in SW480 cells was up-regulated (2.58±0.82) (t=22.17, P<0.01). Compared with CCC-HIE-2 cells (1.46±0.28), the expression of miR-145 in SW480 cells was significantly down-regulated (0.58±0.23) (t=17.86, P<0.01). LncRNA KCNQ1OT1 targets miR-145 and MmiR-145 targets PAK4. After KCNQ1OT1 or PAK4 expression was decreased, SW480 cell viability, clone number, and EMT ability were significantly decreased, and the apoptosis rate was up-regulated. Upregulation of KCNQ1OT1 targeting miR-145 promoted proliferation and EMT of SW480 cells and down-regulated apoptosis rate. Conclusion: Down-regulating LncRNA KCNQ1OT1 inhibited SW480 cell proliferation and EMT through the miR-145/PAK4 axis, and promoted cancer cell apoptosis 2023 Vol. 29 (2): 183-189 [Abstract] ( 147 ) HTML (1 KB)  PDF (2248 KB)  ( 158 ) 189 Effect of miRNA-34a on the Progression of Central Nervous System Lymphoma by Regulating SOX4/RAS/MAPK Signaling Pathway ZHOU Jian, PAN Xiaoran, LI Xiaojuan DOI: 10.3969/j.issn.1006-6233.2023.02.03 Objective: To investigate the role of microRNA-34a (miR-34a) in primary central nervous system lymphoma (PCNSL) and its underlying molecular mechanisms. Methods: The expression of miR-34a in PCNSL tissues was detected by real-time quantitative PCR (RT-qPCR) experiments. Raji cell was cultured in vitro, and miR-34a mimic (miR-34a mimic), mimic control (NC mimic), miR-34a inhibitor (miR-34a inhibitor), inhibitor control (NC inhibitor), miR-34a mimic+Blank plasmid (Vector) and miR-34a mimic+SOX4 overexpression plasmid (pcDNA-SOX4) were transfected into cells respectively. The RT-qPCR assay was used to detect the expression of miR-34a in cells. Cell proliferation ability was detected by CCK-8 assay and clone formation assay. The apoptosis rate was detected by flow cytometry. Western blot was used to detect the expression of SOX4 protein and RAS/MAPK pathway proteins Ras, p-Raf-1, and p-MEK in cells. Bioinformatics software and dual luciferase reporter assay were used to predict and verify the targeting relationship between miR-34a and SOX4, respectively. Results: The expression of miR-34a in PCNSL tissues was significantly decreased (P<0.05). Compared with the NC mimic group, the expression of miR-34a in the miR-34a mimic group was increased, the cell proliferation ability was significantly decreased, the apoptosis rate was significantly increased, the protein expressions of Ras, p-Raf-1, and p-MEK were significantly decreased (P<0.05). Compared with the NC inhibitor group, the expression of miR-34a in the miR-34a inhibitor group was decreased, the cell proliferation ability was significantly increased, the apoptosis rate was significantly decreased, the protein expressions of Ras, p-Raf-1, and p-MEK were significantly increased (P<0.05). Compared with the NC mimic group, the luciferase activity of cells co-transfected with miR-34a mimic and WT-SOX4 was significantly decreased (P<0.05), while there was no significant change in luciferase activity of cells co-transfected with miR-34a mimic and MUT-SOX4 (P>0.05). Compared with the miR-34a mimic+Vector group, the proliferation ability of Raji cells in the miR-34a mimic+pcDNA-SOX4 group was significantly increased, the apoptosis rate was significantly decreased, and the protein expressions of Ras, p-Raf-1, and p-MEK were significantly increased (P<0.05). Conclusion: miR-34a could inhibit PCNSL cell proliferation and promotes apoptosis by targeting SOX4 to regulate the RAS/MAPK signaling pathway. 2023 Vol. 29 (2): 189-194 [Abstract] ( 151 ) HTML (1 KB)  PDF (2493 KB)  ( 149 ) 195 Impact of Hesperetin on D-Galactose-Induced Oxidative Stress Injury in Rats with Cataract by Regulating Keap1/Nrf2/ARE Signaling Pathway LI Qiuxia, et al DOI: 10.3969/j.issn.1006-6233.2023.02.04 Objective: To explore the impact of hesperetin on D-galactose-induced oxidative stress injury in rats with cataract and its mechanism of regulating Keap1/Nrf2/ARE signaling pathway. Methods: Seventy-two SPF Wistar male rats were randomly separated into sham operation group, model group, positive group, low and high dose hesperetin groups, and high dose hesperetin + Nrf2 inhibitor ATRA group. Cataract rat models were established by subcutaneous injection of 200mg/kg of D-galactose in all groups except the sham-operation group. The positive control group was treated with fuming capsule (30mg/kg), the low and high dose groups were treated with hesperetin (50mg/kg, 150mg/kg) respectively, and the high dose +ATRA group was treated with hesperetin (100mg/kg) first, and then ATRA(10mg/kg) was injected intraperitoneally. Slit lamp was used to observe the degree of opacity of the lens tissue in both eyes; HE was applied to observe the morphological changes of lens epithelium in each group; TUNEL method was applied to detect the apoptosis rate of lens epithelial tissue; ELISA was applied to measure superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities in the lens; Western blotting was applied to detect the expression levels of Keap1, Nrf2, and HO-1 proteins in the lens. Results: Compared with the sham-operation group, the lens epithelial tissue of the model group had disordered cell arrangement, edema, and enlarged gaps, the degree of lens opacity, epithelial cell apoptosis rate, Keap1 and cytoplasmic Nrf2 protein expression increased (P<0.05), SOD, CAT, GSH-Px activity, nuclear Nrf2, HO-1 protein expressions decreased (P<0.05). Compared with the model group, the damage of the lens epithelium of the rats in the positive group and the low and high-dose hesperetin groups was alleviated, the degree of lens opacity, epithelial cell apoptosis rate, Keap1 and cytoplasmic Nrf2 protein expression decreased (P<0.05), SOD, CAT, GSH-Px activity, nuclear Nrf2, HO-1 protein expressions increased (P<0.05). The Nrf2 inhibitor ATRA could greatly attenuate the ameliorating effect of high-dose hesperetin on lens damage in cataract rats. Conclusion: Hesperetin can alleviate D-galactose-induced oxidative stress injury in cataract rats, which may be achieved by inhibiting the expression of Keap1 and promoting the activation of Nrf2/ARE pathway. 2023 Vol. 29 (2): 195-200 [Abstract] ( 136 ) HTML (1 KB)  PDF (1926 KB)  ( 169 ) 200 Dexmedetomidine Modulates miR-150-5p/P2X7 Receptor Axis to Attenuate Microglial Activation in Rats with Cerebral Hemorrhage ZHOU Huayong, ZHAO Yuping, YANG Xu, et al DOI: 10.3969/j.issn.1006-6233.2023.02.05 Objective: To investigate the influence of dexmedetomidine (DEX) on the activation of microglia (MG) in rats with intracerebral hemorrhage (ICH) and its regulatory effect on miR-150-5p/P2X7 receptor (P2X7R) axis. Methods: Rats were separated into the sham group (n=12) and the model group (n=63). ICH rat model was established and included in the DEX low-dose group (25μg/kg), DEX high-dose group (50μg/kg), DEX (50μg/kg) +NC antagomir (20nmoL/L, 5μL) group and DEX (50μg/kg) + miR-150-5p antagomir (20nmoL/L, 5μL) group, with 12 rats in each group. The mode of administration was an intraperitoneal injection, once a day, 0.2mL each time, for 7 consecutive days. 24 h after the end of the administration, the neurological function was scored; the serum was separated, and the levels of serum TNF-ɑ and IL-1β were detected by enzyme-linked immunosorbent assay (ELISA); the brain tissue of the bleeding area (CA2 region of hippocampus) was separated, and the brain tissue water content was measured, HE staining was applied to observe histopathological changes, immunofluorescence was applied to observe the morphology of MG, the qRT-PCR method was applied to detect the expression of miR-150-5p and P2X7R mRNA, Western blot method was applied to detect the protein expression of ionic calcium adaptor protein molecule-1 (Iba-1) and purinergic P2X7 receptor (P2X7R), and dual luciferase reporter gene experiment was applied to verify the targeting relationship between miR-150-5p and P2X7R. Results: Compared with the model group, the pathological changes of the brain tissue in the DEX low-dose and high-dose groups were gradually reduced, the infiltration of red blood cells and inflammatory cells was reduced, and the nuclear pyknosis phenomenon was reduced; the number of MG reduced obviously, and the cell body became smaller; the neurological function score and the expression of miR-150-5p in brain tissue were obviously increased (P<0.05); the levels of serum TNF-ɑ and IL-1β, brain tissue water content, and the expression of P2X7R mRNA and protein and Iba-1 protein in brain tissue were obviously reduced (P<0.05). Compared with the DEX high-dose group and DEX+NC antagomir group, a large number of red blood cells and inflammatory cell infiltration could be seen in the DEX+miR-150-5p antagomir group, the brain tissue structure and the arrangement of the cells were disordered; the number of MG was obviously increased, and the cell body became larger; the neurological function score and the expression of miR-150-5p in brain tissue were obviously decreased (P<0.05); the levels of serum TNF-ɑ and IL-1β, brain tissue water content, and the expression of P2X7R mRNA and protein and Iba-1 protein in brain tissue were obviously increased (P<0.05). The results of the dual luciferase reporter gene experiment showed that P2X7R was the downstream target gene of miR-150-5p. Conclusion: DEX can inhibit the activation of MG by up-regulating miR-150-5p and down-regulating P2X7R expression, and then play a protective role in ICH. 2023 Vol. 29 (2): 200-207 [Abstract] ( 111 ) HTML (1 KB)  PDF (1903 KB)  ( 135 ) 208 Effect of Etomidate Modulation of MAPK/ERK Signaling Pathway on Cartilage Damage in Osteoarthritic Rats GAN Zhiqiang, ZHANG Dan, CHEN Yin, et al DOI: 10.3969/j.issn.1006-6233.2023.02.06 Objective: To investigate the effect of etomidate (ET) on cartilage damage in osteoarthritis (OA) rats and its regulation of mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) signaling pathway. Methods: A total of 72 SPF SD male rats were randomly separated into 6 groups: sham operation group, model group, positive group (15mg/kg diclofenac sodium), and ET low (1.5mg/kg), medium (3mg/kg) and high-dose (6mg/kg) groups. The joint edema and weight-bearing asymmetry of the rats in each group were evaluated; the tenderness and thermal pain thresholds of rats in each group were detected; the pathological damage of cartilage in each group was observed by HE and Mankin score; the levels of tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β) and cartilage oligomeric matrix protein (COMP) in rat serum were determined by enzyme-linked immunosorbent assay (ELISA); and the expression of matrix protease-13 (MMP-13) and pathway protein ERK and its phosphorylated p-ERK protein in cartilage tissue were determined by Western blot. Results: Compared with the sham-operation group, the right hind limb knee joint of the model group had edema and increased joint load-bearing asymmetry, cartilage tissue defects, and cartilage layer thinning, and the number of cartilage cells decreased, the levels of serum TNF-α, IL-1β, and COMP increased, the protein expression of MMP-13 and p-ERK1/2, and the ratio of p-ERK1/2/ERK1/2 in cartilage increased (P<0.05). Compared with the model group, the knee joint edema and joint load-bearing asymmetry of the right hind limb in the positive group and the different dose ET groups decreased, the cartilage damage was gradually repaired, the number of cells increased, the cells were evenly arranged, and the cartilage layer became thicker, the levels of serum TNF-α, IL-1β, and COMP decreased, the protein expression of MMP-13 and p-ERK1/2, and the ratio of p-ERK1/2/ERK1/2 in cartilage decreased (P<0.05). Conclusion: ET can alleviate the pathological damage of cartilage tissue in OA rats and reduce the expression of pro-inflammatory factors in serum, which may be achieved by inhibiting the activation of the MAPK/ERK signaling pathway. 2023 Vol. 29 (2): 208-214 [Abstract] ( 125 ) HTML (1 KB)  PDF (2408 KB)  ( 239 ) 214 Protective Effect of CTRP6 on Viral Myocarditis-Associated Cardiac Cell Injury Based on NLRP3 Inflammasome YIN Ruiyuan, et al DOI: 10.3969/j.issn.1006-6233.2023.02.07 Objective: To investigate the role of C1q/tumor necrosis factor-related protein 6 (CTRP6) in mediating viral myocarditis-associated cardiac cell injury induced by Coxsackie B3 virus (CVB3) and explore the potential underlying mechanism. Methods: Human cardiac cell line AC-16 was applied to establish an in vitro model of viral myocarditis by infecting CVB3. CTRP6 overexpression was achieved by transfecting the CTRP6 expression vector. Cells were divided into four groups including the normal group, CVB3 infection group, CVB3 + empty vector group, and CVB3 + CTRP6 vector group. The mRNA expression was measured by real-time quantitative PCR (RT-qPCR). The protein expression was measured by Western blotting. The levels of creatine kinase-MB (CK-MB) and cardiac troponin-I (cTn-I) were assessed by corresponding kits. Cell apoptosis was measured by terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) assay. The concentrations of inflammatory cytokines were quantified by enzyme-linked immunosorbent assay (ELISA). Knockdown of AdipoR1 was achieved by RNAi silencing. Results: As compared with the normal group, infection of CVB3 significantly decreased the expression of CTRP6 in cardiac cells (P<0.01). Infection of CVB3 markedly increased the levels of CK-MB and cTn-I, and significantly enhanced the apoptosis and inflammatory cytokine release in cardiac cells (P<0.01). As compared with the CVB3 + empty vector group, overexpression of CTRP6 significantly downregulated the levels of CK-MB and cTn-I and attenuated the apoptosis and inflammatory cytokine release in cardiac cells infected with CVB3 (P<0.01). Overexpression of CTRP6 also inhibited the activation of NLRP3 inflammasome in CVB3-infected cardiac cells compared with the CVB3 + empty vector group (P<0.01). Silencing of AdipoR1 markedly abolished CTRP6-mediated protective effects on CBV3-induced cardiac cell injury (P<0.01). Conclusion: CTRP6 ameliorates viral myocarditis-associated cardiac cell injury induced by CBV3 through inhibition of NLRP3 inflammasome activation via the action on the receptor AdipoR1. 2023 Vol. 29 (2): 214-220 [Abstract] ( 104 ) HTML (1 KB)  PDF (2320 KB)  ( 163 ) 221 Effects of miR-146a on Brain Development and Cognitive Function in Offspring of Pregnant Rats with Electrical Ignition Seizures MIERGULI Aimaite, et al DOI: 10.3969/j.issn.1006-6233.2023.02.08 Objective: To investigate the effect of miR-146a on the growth and cognitive function of offspring of pregnant rats with complete ignition of epilepsy. Methods: Female rats were divided into control, model, and miR-146a inhibitor groups, with 6 rats in each group. Rats in the control group were implanted with electrodes only, and rats in the model group were implanted with electrodes. Epilepsy was ignited by the constant current with a frequency of 60Hz, wave width of 1.0ms, and string length of 10s. And the rats in the miR-146a inhibitor group were implanted with electrodes, then, epilepsy was ignited, followed by miR-146a inhibitor administration (dose: 2 mg/kg), twice a week until delivery. The female rats were caged together with the male rats after successful seizure ignition, and the female rats were observed to be pregnant. After the litter was born, the timing of the roll-over reflex and grasp response was observed during the growth of the litter, and the miR-146a content in brain tissue was compared by RT-PCR. The litter was monitored for body weight and length, and after the litter was grown to 84 d, the Morris water maze test was performed to assess the cognitive function of the litter. Nissler staining was used to detect the neuronal status and Golgi staining to detect the dendritic spine density. Results: Compared with the control group, the content of miR-146a in the model group increased (P<0.01); compared with the model group, the content of miR-146a in the miR-146a inhibitor group decreased(P<0.01). Compared with the control group, the offspring rats in the model group of the righting reflex time increased(P<0.01), the grasping reaction time decreased (P<0.01), the body weight and body length reduced(P<0.001), the escape latency significantly increased (P<0.01), and the number of crossing the platform and swimming in the platform quadrant significantly decreased(P<0.01); the number of Nissl cells decreased, and the length and density of dendritic spines decreased (P<0.01). Compared with the model group, the offspring rats in the miR-146a inhibitor group increased, the righting reflex time decreased (P<0.001), and the grasping reaction time prolonged (P<0.01); the body weight and body length of the offspring increased (P<0.01), the escape latency reduced (P<0.01), the number of platform crossings and platform quadrant swimming increased (P<0.05); the number of Nissl cells increased, and the length and density of dendritic spines increased (P<0.05). Conclusion: The complete ignition of maternal epilepsy during pregnancy can increase the expression of miR-146a in the brain tissue of offspring rats. Reducing the expression of miR-146a can significantly promote the growth of offspring rats, promote the development of the central nervous system, increase the density and length of dendritic spines, and improve the long-term development of offspring rats’ cognitive function 2023 Vol. 29 (2): 221-226 [Abstract] ( 92 ) HTML (1 KB)  PDF (1857 KB)  ( 140 ) 226 Probing the Role of miR-653 in Stemness Maintenance of Lung Cancer Stem Cells Based on miRNA Regulatory Network HE Da, MA Ziyue, XI Junfeng DOI: 10.3969/j.issn.1006-6233.2023.02.09 Objective: To explore the role and related mechanism of miR-653 in the maintenance of stemness of lung cancer stem cells based on the miRNA regulatory network. Methods: miRNA-seq was used to detect tumor tissues and adjacent tissues of lung cancer patients, and the regulatory network was drawn according to the difference of miRNA, and the target genes were analyzed by KEGG analysis. miR-NC, miR-653, oe-NC, and oe-MYC were transfected into the H460 lung cancer cell line by liposome transfection method. The cells were divided into the miR-NC group, miR-653 group, miR-653+oe-NC group, and miR-653+oe-MYC group. The expression of miR-653 and MYC were detected by qRT-PCR. The protein expression of CD133 and CD24 was detected by flow cytometry. CCK8 was used to detect cell proliferation. The proportion of pellet formation was detected by the limited dilution method. The targeting of miR-653 and MYC was detected by a luciferase reporter assay kit. Results: Compared with adjacent tissues, 245miRNAs were up-regulated and 363 miRNAs were down-regulated in tumor tissues, among which miR-653 was significantly down-regulated (P<0.05). The regulatory network predicted that miR-653 targeted 22 miRNAs and was involved in signaling pathways such as cell stemness maintenance. The fluorescence intensity of the miR-653 group was lower than that of the miR -NC group in wild-type MYC (P<0.05). Compared with the miR-NC group, the expression of miR-653 was increased and the expression of MYC was decreased in the miR-653 group. The protein expression of CD133 and CD24 was decreased, and the proportion of cell proliferation and pellet formation ratio was decreased (P<0.05). Compared with the miR-653+oe-NC group, the expression of MYC in the miR-653+oe-MYC group was increased. The protein expression of CD133, and CD24 was increased, and cell proliferation and pellet formation ratio was increased (P<0.05). Conclusion: The miRNA regulatory network is involved in the stemness maintenance of lung cancer cells, and miR-653 regulates MYC to inhibit the maintenance of lung cancer cell stem cells. 2023 Vol. 29 (2): 226-232 [Abstract] ( 116 ) HTML (1 KB)  PDF (1884 KB)  ( 176 ) 232 Study on the Expression of Mir-191-5p in Plasma and Its Correlation with Renal Interstitial Fibrosis in Patients with IgA Nephropathy XIA Weiqing, SONG Juxiang, YANG Ermei, et al DOI: 10.3969/j.issn.1006-6233.2023.02.010 Objective: To investigate the expression of miR-191-5p in the plasma of patients with IgA nephropathy and its correlation with renal interstitial fibrosis (RIF). Methods: 80 patients with IgA nephropathy (IgA nephropathy group) admitted to our hospital from February 2017 to February 2022 were divided into stage Ⅰ~Ⅱ group (42 cases), stage Ⅲ group (38 cases) according to CKD stages. Patients were divided into the T0 group (n = 20), T1 group (n = 39), and T2 group (n = 21) according to Oxford parting, and 77 healthy volunteers were selected as the control group. Plasma miR-191-5p expression was detected by Quantitative real-time polymerase chain reaction (qRT-PCR), renal function [creatinine (Scr), urea nitrogen (BUN)] were detected by the automatic biochemical analyzer, and RIF indexes [transforming growth factor TGF -β1 and TGF-α]were detected by enzyme-linked immunosorbent assay. Pearson analysis was conducted to understand the correlation between miR-191-5p expression and renal function and RIF index and RIF area. Results: The levels of serum miR-191-5P in the IgA nephropathy group were lower than those in the control group (P<0.05), and the levels of Scr, BUN, TGF -β1, and TGF-α were higher than those in the control group (P<0.05). The expression of miR-191-5p in the stage Ⅲ group was lower than that in the stageⅠ~Ⅱ group (P<0.05), Scr, BUN, TGF-β1, and the area of RIF was higher than that in stageⅠ~Ⅱ group (P<0.05). The expression of miR-191-5p in the T2 group was lower than that in the T1 group and T0 group (P<0.05), Scr, BUN, TGF-β1, and the area of RIF was higher than that in the T1 group and T0 group (P<0.05). Plasma miR-191-5p expression was negatively correlated with RIF area, Scr, BUN, TGF-β1, and TGF-α in patients with IgA nephropathy (r=-0.509, -0.379, -0.311, -0.475, -0.486,P<0.05). Conclusion: The expression of miR-191-5p in plasma is decreased in patients with IgA nephropathy and is related to RIF and renal function decline. 2023 Vol. 29 (2): 232-236 [Abstract] ( 108 ) HTML (1 KB)  PDF (1220 KB)  ( 130 ) 236 Study on the Effect of Sodium Aescinate Combined with Bone Peptide Injection on Limb Swelling and Serum BALP and BGP in Elderly Patients after Calcaneal Fracture Surgery XIE Ting, LI Ya, WANG Ning, et al DOI: 10.3969/j.issn.1006-6233.2023.02.011 Objective: To investigate the effect of sodium aescinate combined with bone peptide injection on limb swelling after calcaneal fracture surgery in elderly patients and the influence of serum bone alkaline phosphatase (BALP) and Osteocalcin (BGP). Methods: A total of 102 elderly patients with limb swelling after calcaneal fracture treated in our hospital from April 2018 to October 2020 were selected and divided into 2 groups using random number table method. The control group received sodium aescinate 10mg,qd; the treatment group was treated with sodium aescinate 10mg, qd combined with bone peptide injection 10ml, qd, and the 2 groups were treated for 2 weeks. The leg circumference difference, limb swelling score, visual analogue scale (VAS) score, serum BALP and BGP levels were recorded, and the clinical efficacy was compared between the two groups. Results: Compared with the clinical effective rate of 86.27% in the control group, the effective rate of the experimental group was 96.08% (P<0.05). Compared with before treatment, the difference of calf circumference, swelling score and VAS score in the two groups were decreased after treatment. Compared with the control group, the difference of calf circumference difference, swelling score and VAS score before and after treatment in the experimental group were higher, and the differences were statistically significant (P<0.05). Compared with before treatment, serum BALP and BGP levels increased after treatment (P <0.05). Compared with the control group, the differences in serum BALP and BGP levels before and after treatment in the experimental group were higher, and the differences were statistically significant (P<0.05). Conclusion: Sodium aescinate combined with bone peptide injection has definite curative effect on limb swelling after calcaneal fracture surgery in the elderly, can improve bone metabolism, and has high safety, which is worthy of promotion. 2023 Vol. 29 (2): 236-242 [Abstract] ( 123 ) HTML (1 KB)  PDF (1238 KB)  ( 273 ) 242 Study on the Pharmacological Mechanism of Fici Hirtae Radix in Treating Myasthenia Gravis based on Network Pharmacology and Molecular Docking ZHANG Hanlin, YU Jieying, TIAN Enwei DOI: 10.3969/j.issn.1006-6233.2023.02.012 Objective: To predict the active components and targets of Fici Hirtae Radix in the treatment of Myasthenia Gravis ( MG )using network pharmacology and molecular docking technology, and to explore its potential mechanism. Methods: The intersection targets between the action targets of Fici Hirtae Radix and the disease targets of MG were obtained through TCMSP, SwissADME, OMIM, GeneCards and other databases, and the interactions between the targets were resolved and PPI network diagrams were constructed using the STRING database platform. Active ingredient-disease target network analysis was conducted using Cytoscape 3.9.0 software, based on which functional enrichment analysis of GO and KEGG genes was performed using David's database. After screening the key active ingredients and target genes, the active ingredients were molecularly docked to the important targets using Autodock vina and PyMol software to verify the binding activity. Results: Eight effective active components with targets were screened from Fici Hirtae Radix, and eight possible protein targets of TNF, IL6, EGFR and IL1 B in the treatment of MG were predicted. By mediating TNF, Toll-like cell receptor, PI3K-Akt, MAPK and other signaling pathways, anti-inflammatory and improving their immune ability were produced, so as to achieve the occurrence and progress of myasthenia gravis, and the activity between components and targets was preliminarily verified. Conclusion: The characteristics of multi-component, multi-target and multi-pathway in the treatment of MG were revealed by network pharmacology and molecular docking technology, and the possible targets and signal transduction mechanisms of Fici Hirtae Radix in the treatment of myasthenia gravis were explored. 2023 Vol. 29 (2): 242-249 [Abstract] ( 180 ) HTML (1 KB)  PDF (2916 KB)  ( 229 ) 249 Clinical Efficacy Analysis of Argatroban Combined with Butylphthalide in the Treatment of Acute Ischemic Cerebral Infarction in the Elderly WANG Jihui, ZHOU Changhu, CHEN Lingdong DOI: 10.3969/j.issn.1006-6233.2023.02.013 Objective: To observe and analyze the clinical efficacy of argatroban combined with butylphthalide in the treatment of acute ischemic cerebral infarction in elderly patients, and to study its safety. Methods: Using 130 cases of clearly diagnosed acute ischemic cerebral infarction in the elderly admitted to our hospital from March 2019 to September 2021 as the study subjects, a simple randomized grouping method was used with a random number table, in which 65 patients in the control group were treated with argatroban (day 1 and 2, 60 mg/d argatroban 24 h continuous intravenous pumping, day 3, 4 and 5, argatroban 10mg/d, 3 h /The Barthel Index, NIHSS and mRS scores were measured before and after treatment, and the R-value, K-value, α-angle and MA-value of the thromboelastogram were measured in 65 patients in the test group (same as the control group). MA values and other indicators were measured. The efficacy of the treatment in both groups was calculated and adverse effects such as dizziness, loss of appetite and nausea during the treatment were counted. Results: Before treatment, the differences in NIHSS scores, Barthel Index scores and mRS scores of patients treated by different regimens were not statistically significant (P>0.05); after treatment, the NIHSS scores and mRS scores of patients in both groups were significantly lower, and the differences in NIHSS scores (8.34±1.24) and mRS scores (1.60±0.12) of patients in the test group before and after treatment were significantly higher than those in the control group (5.07±1.14, 0.73±0.09), with statistically significant differences (P<0.05); the Barthel Index scores of patients were significantly higher after treatment, and the difference in Barthel Index scores before and after treatment was significantly higher in the test group (17.58±5.41) than that in the control group (9.70±4.22), with statistically significant differences (P<0.05). The difference was statistically significant (P<0.05). Before treatment, there was no significant difference in the thromboelastography indexes (R value, K value, α angle and MA value) between the two groups (P>0.05); after treatment, R value and K value increased significantly, α angle and MA value decreased significantly in the two groups, and α angle was smaller in the test group than in the control group, the increase in R value before and after treatment was significantly lower in the test group (0.16±0.12) than in the control group (0.20±0.11) The increase in R-value (0.16±0.12) was significantly lower than that of the control group (0.20±0.11), and the decrease in alpha angle (9.40±0.88) was significantly higher than that of the control group (7.46±0.75) before and after treatment. The overall effective rate of patients in the test group was 90.77%, which was significantly higher than that of patients in the control group (70.77%), and the difference was statistically significant (P<0.05). The main adverse reactions of patients were dizziness, loss of appetite and nausea, with a total of 5 cases (7.69%) in the trial group and 4 cases (6.15%) in the control group (P>0.05). Conclusion: Agatroban combined with butylphthalide in the treatment of acute ischaemic cerebral infarction in the elderly significantly reduced the NIHSS score and mRS score of the patients, and the Barthel Index score of the patients was significantly higher, which had a better prognosis than treatment with agatroban alone and facilitated clinical treatment. 2023 Vol. 29 (2): 249-254 [Abstract] ( 144 ) HTML (1 KB)  PDF (1227 KB)  ( 204 ) 254 Study on the Relationship between Serum NLR LMR NMR and Prognosis of Esophageal Cancer Patients HU Junze, XIE Quan, MA Jianwei, et al DOI: 10.3969/j.issn.1006-6233.2023.02.014 Objective: To analyze the relationship between serum neutrophil-lymphocyte ratio (NLR), lymphocyte-monocyte ratio (LMR), neutrophil-monocyte ratio (NMR), and prognosis in patients with esophageal cancer. Methods: A total of 116 patients with esophageal cancer undergoing radical resection in the hospital were enrolled as an observation group between January 2017 and January 2021, while other 116 healthy controls during the same period were enrolled as the control group. The levels of white blood cell count (WBC), neutrophils, lymphocytes, and monocytes were detected by a hematology analyzer. NLR, LMR, and NMR were analyzed. The 12-month prognosis of patients with esophageal cancer was statistically analyzed. The patients with recurrence increased staging, other organ metastasis, and death was enrolled as a poor prognosis group, while the remaining were enrolled as a good prognosis group. The predictive value of NLR, LMR, and NMR for the prognosis of patients with esophageal cancer was analyzed by receiver operating characteristic (ROC) curves. Results: There was no significant difference in levels of WBC, neutrophils, and lymphocytes between the two groups (P>0.05). The level of monocytes in the observation group was lower than that in the control group (P<0.05). There was no significant difference in NLR between the two groups (P>0.05). LMR in the observation group was significantly lower than that in the control group, while NMR was significantly higher than that in the control group (P<0.05). There was no significant difference in NLR between the stage I+II group and the stage III group. LMR in the stage I+II group was higher than that in the stage III group, while NMR was lower than that in the stage III group (P<0.05). Of the 116 patients with esophageal cancer, there were 32 cases (27.85%) with poor prognoses. The levels of serum NLR and NMR in the poor prognosis group were significantly higher than those in the good prognosis group, while LMR was significantly lower than that in the good prognosis group (P<0.05). AUC values of serum NLR, LMR, and NMR for predicting the prognosis of esophageal cancer were 0.766, 0.799, and 0.708, respectively (P<0.05). Taking 2.670, 4.379, and 15.896 as the cut-off values of NLR, LMR, and NMR, the sensitivity and specificity of combined detection (parallel mode) for predicting prognosis were 93.75% and 64.29%, respectively. The sensitivity of combined detection was higher than that of a single index. Conclusion: Serum NLR, LMR, and NMR are related to the prognosis of patients with esophageal cancer. The combined detection of serum NLR, LMR, and NMR levels have important clinical value in diagnosing esophageal cancer and predicting 1-year prognosis. 2023 Vol. 29 (2): 254-259 [Abstract] ( 134 ) HTML (1 KB)  PDF (1434 KB)  ( 232 ) 259 Expressions and Significance of P120ctn, p53 and CK5/6 in Tissues of Patients with Invasive Breast Carcinoma LIN Weiqiang, YUAN Huiling, WEN Runyao, et al DOI: 10.3969/j.issn.1006-6233.2023.02.015 Objective: To investigate the expressions and clinical significance of P120 catenin (P120ctn), p53 protein, and cytokeratin 5/6 (CK5/6) in cancer tissues of patients with invasive breast carcinoma (IBC). Methods: The clinical data of 120 patients with IBC were retrospectively analyzed between October 2018 and October 2021. Immunohistochemistry was used to detect the expressions of CD31, p53, and CK5/6 in cancer tissues and adjacent tissues, and their relationship with the clinicopathological characteristics and preoperative neoadjuvant chemotherapy (NAC) efficacy were analyzed. Results: The positive expression rates of P120ctn, p53, and CK5/6 in cancer tissues were 81.67%, 75.83%, and 38.33%, which were significantly higher than 55.00%, 15.00%, and 3.25% in adjacent tissues (all P<0.05). P120ctn expression was correlated with molecular typing and lymph node metastasis (P<0.05), p53 was related to tumor diameter, molecular typing, histological grading, lymph node metastasis, and clinical staging (P<0.05), and CK5/6 was associated with molecular typing and histological grading (P<0.05). Spearman rank correlation analysis showed that the expressions of P120ctn, p53, and CK5/6 in cancer tissues were positively correlated with each other (r=0.384, 0.406, 0.581, allP<0.05). After 2 cycles of NAC for 120 patients before surgery, the effective rate was 63.33% (76/120). Univariate analysis showed that the effective rate was lower in patients with tumor diameter>2cm, histological grade G3, lymph node metastasis, P120ctn positive expression, p53 positive expression, and CK5/6 positive expression (P<0.05). Multivariate Logistic regression analysis revealed that lymph node metastasis, positive P120ctn expression, and positive p53 expression were risk factors affecting the efficacy of NAC in patients (P<0.05). Conclusion: The expressions of P120ctn, p53, and CK5/6 in cancer tissues of patients with IBC are significantly increased, and they are related to the clinicopathological characteristics of patients. The positive expressions of P120ctn and p53 are influencing factors for the efficacy of preoperative NAC. 2023 Vol. 29 (2): 259-265 [Abstract] ( 157 ) HTML (1 KB)  PDF (1241 KB)  ( 301 ) 265 Relationship between Serum 25-(OH)-D3, hs-CRP and ECG Parameters and Malignant Arrhythmia in Patients with STEMI after Interventional Therapy LI Yonghui, WANG Xiaoling, YUAN WenLong, et al DOI: 10.3969/j.issn.1006-6233.2023.02.016 Objective: To investigate the relationship between the changes of serum 25 hydroxyvitamin D3 (25-(OH)-D3), high sensitivity C-reactive protein (hs CRP), ECG parameters, and malignant arrhythmia after percutaneous coronary intervention (PCI) in patients with ST-segment elevation myocardial infarction (STEMI). Methods: A total of 193 STEMI patients who underwent PCI in our hospital from January 2020 to June 2022 were selected as the research subjects. According to whether the patients had malignant arrhythmias within 72 hours after surgery, they were divided into the arrhythmia group of 38 cases and the control group of 155 cases. The 25-(OH)-D3, hs-CRP, serum electrolytes, blood routine indexes, blood lipid indexes, and myocardial enzyme indexes of the patients in the groups were compared within 12 hours of admission, and the patients were monitored by continuous dynamic electrocardiogram after PCI. The fragmented QRS complex, corrected Tp-e interval (Tp-ec), QT dispersion (Qtd), and logistic regression model were used to analyze the relationship between the above indicators and the occurrence of malignant arrhythmia in STEMI patients after PCI. Results: The level of serum 25-(OH)-D3 in patients with arrhythmia was lower than that in the control group. The detection rates of hs CRP, Tp-ec, Qtd, and fragmented QRS waves in patients with arrhythmia were higher than those in the control group. The differences between the above indicators were statistically significant (P<0.05); the levels of white blood cell (WBC), troponin I (cTnI), creatine kinase isoenzyme (CK-MB), myoglobin (MYO), K+, Mg2+; logistic regression model showed that cTnI, CK-MB, potassium ion, hs CRP, Tp-ec, and Qtd increased, and positive fragmented QRS wave significantly increased the risk of malignant arrhythmia in STEMI patients after PCI (P<0.05); the increase of serum 25-(OH)-D3 level will reduce the risk of malignant arrhythmia in STEMI patients after PCI (P<0.05). Conclusion: The decrease of serum 25-(OH)-D3 level, the increase of hs-CRP, the increase of Tp-ec and Qtd after PCI, and the occurrence of the fragmented QRS complex in STEMI patients will increase the risk of malignant arrhythmia in patients. 2023 Vol. 29 (2): 265-270 [Abstract] ( 123 ) HTML (1 KB)  PDF (1232 KB)  ( 128 ) 270 Efficacy of Kushen Decoction Bath Combined with Black Light in the Treatment of Psoriasis Vulgaris JIANG Hongling, CHEN Xi, et al DOI: 10.3969/j.issn.1006-6233.2023.02.017 Objective: To observe the efficacy of the Kushen decoction bath combined with black light in the treatment of psoriasis vulgaris. Methods: A simple random grouping method using a random number table was used to divide 102 patients with psoriasis admitted to our hospital from February 2020 to February 2021 into 51 cases each in the observation and control groups. The control group was given black light therapy, while the observation group was treated with a Kushen decoction bath combined with black light therapy. The clinical efficacy of the two groups of patients was compared, and the disease symptoms [Psoriasis Area and Severity Index (PASI), Pruritus Visual Analogue Scale (VAS)], serum-related factors [CC chemokine ligand 20(CCL20), transforming growth factorβ1(TGF-β1), interleukin 17(IL-17), tumor necrosis factor-α(TNF-α)] and levels of T lymphocyte subsets (CD3+, CD4+, CD8+, CD4+/CD8+) were compared before and after treatment. Results: After 2 weeks of treatment, the total clinical efficiency of the observation group (98.53%) was higher than that of the control group (88.24%) (P<0.05). After 2 weeks of treatment, the PASI score, VAS score, and levels of serum-related factors (CCL20, IL-17, TNF-α, TGF-β1) and CD8+ in the two groups were lower than those before treatment, and the differences before and after treatment showed statistical significances between the groups (P<0.05). The levels of CD3+, CD4+, and CD4+/CD8+ in both groups were higher compared to before treatment, and the differences before and after treatment were statistically significant between the groups (P<0.05). Conclusion: Compared with black light irradiation alone, Kushen decoction bath combined with black light irradiation therapy has a more significant efficacy on psoriasis vulgaris, and has a more positive significance in regulating the body’s inflammatory status and immune function, alleviating the immune damage and promoting the skin repair. 2023 Vol. 29 (2): 270-275 [Abstract] ( 148 ) HTML (1 KB)  PDF (1600 KB)  ( 124 ) 275 Effects of Bio-Oss Bone Meal Combined with PRF and GBR Technology on Peri-Implant Bone Regeneration and Peri-Implantitis HUANG Xuchen, et al DOI: 10.3969/j.issn.1006-6233.2023.02.018 Objective: To explore the effect of Bio-Oss bone meal combined with PRF and GBR technology on peri-implant bone regeneration and peri-implantitis. Methods: A total of 90 patients who underwent oral implant-guided bone regeneration in our hospital from February 2020 to April 2022 were selected as the research subjects, and divided into 3 groups according to the order of admission. Thirty cases in the Bio-Oss group were given Bio-Oss bone meal combined with guided bone regeneration (GBR) for guided regeneration, 30 cases in the PRF group were given platelet-rich fibrin (PRF) combined with GBR technique for guided regeneration, and 30 cases in the combined group were given Bio-Oss bone meal Combined PRF and GBR technology to guide regeneration. The peri-implant bone regeneration at 1 month and 3 months after operation among the three groups, pain at 24 hours after the operation, mucosal swelling at the surgical site at 1 week after the operation, and the plaque index (PLI) before surgery, 1 month, and 3 months after surgery probing depth (PD), and postoperative complications were compared among the three groups. Results: At 1 month and 3 months after the operation, the height of the bone graft and the thickness of bone formation in the combined group were significantly higher than those in the Bio-Oss group and the PRF group (P<0.05). There was no significant difference in the bone graft height and bone formation thickness between the Bio-Oss group and the PRF group (P>0.05); 24 hours after the operation, the pain in the combined group was significantly better than that in the other two groups (P<0.05), while there was no significant difference between the other two groups (P>0.05). One week after the operation, the mucosal swelling in the combined group was significantly lighter than that in the Bio-Oss group and the PRF group (P<0.05), while there was no significant difference between the Bio-Oss and PRF groups (P>0.05). At 1 month and 3 months after the operation, the PLI and PD of the three groups were significantly lower than those before the operation (P<0.05). And the PLI score and PD of the combined group were significantly lower than those of the Bio-Oss group and the PRF group(P<0.05). However, there was no significant difference between the PRF group and the Bio-Oss group (P>0.05). There was no significant difference in the total incidence of postoperative complications among the three groups (P>0.05). Conclusion: The application of Bio-Oss bone powder combined with PRF and GBR technology in oral implant-guided bone regeneration can help promote postoperative bone defect regeneration, reduce postoperative pain, promote soft tissue regeneration, reduce mucosal swelling, and promote obvious peri-implant soft tissue inflammation improved, and the safety is high, which is worthy of clinical application. 2023 Vol. 29 (2): 275-280 [Abstract] ( 147 ) HTML (1 KB)  PDF (1220 KB)  ( 274 ) 280 Predictive Value of sLOX-1, HIF-1α and IGF-1 on Delayed Cerebral Ischemia after Aneurysmal Subarachnoid Hemorrhage JIANG Haiyang, CHEN Hu, et al DOI: 10.3969/j.issn.1006-6233.2023.02.019 Objective: To explore the predictive value of soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1), hypoxia-inducible factor-1α (HIF-1α), and insulin-like growth factor-1 (IGF-1) on delayed cerebral ischemia (DCI) after aneurysmal subarachnoid hemorrhage (aSAH). Methods: A total of 104 patients with aSAH in the hospital from March 2019 to March 2022 were selected as the research subjects. According to the occurrence of DCI, the patients were divided into a DCI group and a non-DCI group. The general clinical data [triacylglycerol (TG), intracranial pressure (ICP), Hunt-Hess grading] and serum sLOX-1, HIF-1α, and IGF-1 levels were compared between the two groups. Pearson correlation analysis was used to analyze the correlation between serum sLOX-1, HIF-1α, IGF-1 and TG, ICP, and Hunt-Hess grading. The receiver operating characteristic (ROC) curve was drawn to assess the predictive efficiency of serum sLOX-1, HIF-1α, and IGF-1 on DCI after aSAH. Results: The TG, ICP and Hunt-Hess grading in the DCI group were higher than those in the non-DCI group (P<0.05). The levels of serum sLOX-1 and HIF-1α in the DCI group were higher than those in the non-DCI group (P<0.05) while the level of IGF-1 was lower than that in the non-DIC group (P<0.05). Pearson correlation analysis showed that serum sLOX-1 and HIF-1α levels were positively correlated with TG, ICP, and Hunt-Hess grading (P<0.05), but serum IGF-1 level was negatively correlated with TG, ICP, and Hunt-Hess grading (P<0.05). ROC curve showed that the sensitivities and specificities of serum sLOX-1, HIF-1α, IGF-1 alone and in combination on predicting DCI after aSAH were 87.10%/79.50%, 90.30%/83.60%, 83.90%/79.50%, and 96.80%/78.10% respectively, and the corresponding AUCs were 0.894, 0.920, 0.886, and 0.975 respectively. Conclusion: sLOX-1, HIF-1α, and IGF-1 are related to the occurrence of DCI after aSAH, and combined detection can predict DCI after aSAH to a certain extent. 2023 Vol. 29 (2): 280-284 [Abstract] ( 81 ) HTML (1 KB)  PDF (1346 KB)  ( 91 ) 285 Analysis of the Relationship between Left Ventricular Systolic Function and Cardiac Function Grading and Liver Stiffness in Patients with Liver Cirrhosis GUO Hongli, LIANG Hongliang, ZHANG Tao, et al DOI: 10.3969/j.issn.1006-6233.2023.02.020 Objective: To explore the relationship between left ventricular systolic function and cardiac function grading and liver stiffness in patients with liver cirrhosis. Methods: From July 2020 to June 2022, 47 patients with liver cirrhosis were selected as the research subjects, and 40 healthy subjects undergoing physical examination were enrolled as the control group. The cardiac function indicators (LVEDD, LVESD, LVEDV, LVESV, LVEF) were detected by iu-Ilite ultrasound diagnostic apparatus (Philips Company, Netherlands), and aixplorer color Doppler ultrasound was adopted to detect the liver stiffness, and the correlation of left ventricular systolic function indicators with cardiac function grading and liver stiffness was analyzed. Results: The LVEDV and LVESV of the liver cirrhosis group were higher than those of the control group [(96.04±7.73) mL vs (91.26±7.89) mL, (36.53±7.12) mL vs (31.02±7.58) mL] while the LVEF was lower than that of the control group [(0.62±0.08)% vs (0.66±0.10)%,P<0.05]. According to the different NYHA cardiac function grading in patients with liver cirrhosis, they were divided into grade I (20 cases), grade II (16 cases), grade III (11 cases), and grade IV (5 cases). The LVEDD, LVEDV, and LVESV of patients with NYHA cardiac function grades III and IV were greater than those of patients with grades I and II [(4.58±0.38) cm vs (4.34±0.35) cm, (98.52±6.49) mL vs (94.24±7.05) mL, (39.06±6.34) mL vs (35.22±6.05) mL] while the LVEF was less than that of patients with grades I and II [(0.60±0.04)% vs (0.63±0.05)%,P<0.05].The LVESV of patients with liver cirrhosis was positively correlated with cardiac function grading while the LVEF was negatively correlated with cardiac function grading (r=0.198, -0.206,P<0.05). According to Child-Pugh grading, patients with liver cirrhosis were classified into grade A (15 cases), grade B (20 cases), and grade C (12 cases), and the LVEDV, LVESV, and liver stiffness of patients with liver cirrhosis were increased with the increase of liver function grading, while the LVEF was decreased with the increase of liver function grading (P<0.05). LVESV was positively correlated with liver stiffness, and LVEF was negatively correlated with liver stiffness in patients with liver cirrhosis (P<0.05). Conclusion: The left ventricular systolic function is decreased in patients with liver cirrhosis, and the left ventricular systolic function indicators are related to cardiac function grading and liver stiffness. 2023 Vol. 29 (2): 285-289 [Abstract] ( 101 ) HTML (1 KB)  PDF (1212 KB)  ( 193 ) 289 Value of PCT and hs-CRP Combined with Clinical Pulmonary Infection Score in Prognosis Evaluation of Elderly Patients with Severe Pneumonia WANG Yilv, WANG Zhen, ZHONG Wen, et al DOI: 10.3969/j.issn.1006-6233.2023.02.021 Objective: To observe the clinical value of serum procalcitonin (PCT) and high-sensitivity C-reactive protein (hs-CRP) combined with clinical pulmonary infection score (CPIS) in prognosis evaluation of elderly patients with severe pneumonia (SP). Methods: This study included 92 elderly patients with severe pneumonia (severe group) and 30 elderly patients with ordinary pneumonia (ordinary group) who were admitted to the hospital from April 2020 to March 2022, and 30 elderly patients without major diseases (control group). Serum PCT and hs-CRP levels, and CPIS were compared among the groups. According to the survival status within 1 month after admission, patients in the severe group were divided into a survival group and a death group. Serum PCT and hs-CRP levels, and CPIS in patients with different prognoses were observed. The receiver operating characteristic (ROC) curve was used to analyze the prognostic value of serum PCT, and hs-CRP combined with CPIS in elderly patients with SP. Results: Serum PCT and hs-CRP levels, and CPIS in the severe group at admission were significantly higher than those in the ordinary group and the control group (P<0.05). Serum PCT and hs-CRP levels, and CPIS in the death group were significantly higher than those in the survival group (P<0.05). ROC curve analysis showed that the best cut-off values of serum PCT, hs-CRP, and CPIS were 9.595ng/mL, 17.615mg/L, and 8.175. All three could be used for the prognosis of elderly patients with SP (P<0.05). The sensitivity of the combination of the three in the prognosis of elderly patients with SP was higher than that of each index (P<0.05). Conclusion: Compared with patients with ordinary pneumonia and healthy elderly people, elderly patients with SP have higher serum PCT and hs-CRP levels, and higher CPIS. Combined detection of the three is of high value in the prognosis of elderly patients with SP. Therefore, close monitoring of the three indicators is recommended in clinical practice. 2023 Vol. 29 (2): 289-293 [Abstract] ( 123 ) HTML (1 KB)  PDF (1327 KB)  ( 99 ) 293 Efficacy and Safety of Emergency ERCP on Elderly Patients with Acute Cholangitis of Severe Type Secondary to Common Bile Duct Stones WANG Kai, ZHANG Jian, ZHANG Shilun DOI: 10.3969/j.issn.1006-6233.2023.02.022 Objective: To explore the efficacy and safety of emergency endoscopic retrograde cholangiopancreatography (ERCP) in the treatment of elderly patients with acute cholangitis of severe type (ACST) secondary to common bile duct stones. Methods: The clinical medical records of 105 elderly patients with ACST secondary to common bile duct stones admitted to the hospital from August 2020 to April 2022 were retrospectively reviewed, and according to different surgical procedures, they were divided into observation group (n=73, ERCP) and control group (n=32, laparoscopic choledochal exploration lithotomy). The surgical conditions, clinical efficacy, laboratory indicators, and incidence rates of surgical complications were compared between the two groups. Results: The duration of surgery, average intraoperative bleeding, postoperative hospital stay, and abdominal pain relief time in the observation group were all shorter or less than those in the control group (P<0.05). The levels of serum total bilirubin (TBIL), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were decreased in the two groups at 3 days after surgery compared to before surgery (P<0.05), but there were no statistical differences in the levels of TBIL, ALT and AST between the two groups before and after surgery (P>0.05). Compared with before surgery, the white blood cell count and serum high mobility group box 1 (HMGB1) in the two groups were reduced 3 days after surgery while the proportion of neutrophils was enhanced, and the range of changes in the observation group was greater than those in the control group (P<0.05). There was no statistical significance in the total incidence rate of surgical complications between the two groups (P>0.05). Conclusion: Emergency ERCP has good clinical effects in the treatment of elderly patients with ACST secondary to common bile duct stones. It has the advantages of significantly shortening the surgical time, reducing the intraoperative bleeding volume, rapidly relieving abdominal pain symptoms, alleviating systemic inflammatory reactions, and has certain safety. Therefore, it can be used as the preferred surgical method in clinical practice. 2023 Vol. 29 (2): 293-298 [Abstract] ( 92 ) HTML (1 KB)  PDF (1229 KB)  ( 114 ) 298 Correlation between Glycosylation Gap and Type 2 Diabetic Retinopathy SUN Xingxing, et al DOI: 10.3969/j.issn.1006-6233.2023.02.023 Objective: To analyze the correlation between glycosylation gap (GG) and diabetic retinopathy (DR) in patients with type 2 diabetes mellitus (T2DM). Methods: A total of 90 patients with T2DM were admitted to the hospital from September 2021 to April 2022. They were divided into the T2DM group (n=30) and T2DR group (n=60) according to the presence or absence of DR. Univariate analysis and multivariate analysis were performed to screen the risk factors of DR, and the ROC curve was plotted to analyze the predictive value of GG for DR in patients with T2DM. Results: Linear regression analysis showed that there was a linear regression relationship between HbA1c and FA (P<0.05). Univariate analysis showed that blood urea nitrogen (BUN), serum albumin (ALB), HbA1c, and GG were significantly different in the 2 groups (P<0.05). Multivariate logistic regression analysis showed that GG was a risk factor for DR in patients with T2DM (P<0.05). The ROC analysis results showed that the AUC of GG for predicting DR was 0.777. Conclusion: GG is a risk factor for DR in patients with T2DM, and maybe a marker for predicting the occurrence of DR. 2023 Vol. 29 (2): 298-302 [Abstract] ( 70 ) HTML (1 KB)  PDF (1242 KB)  ( 113 ) 302 Effect of Preserving the Left Colonic Artery during Laparoscopic Low Anterior Resection on the Outcome and Prognosis of Patients with Rectal Cancer CHEN Xiaobao, MEI Tianming, WEI Jun, et al DOI: 10.3969/j.issn.1006-6233.2023.02.024 Objective: To explore the effect of preserving the left colonic artery (LCA) during laparoscopic low anterior resection on patients with rectal cancer. Methods: The clinical data of 85 patients undergoing laparoscopic low anterior resection of rectal cancer in the hospital between April 2018 and February 2021 were retrospectively analyzed. According to different treatment methods, the patients were divided into the observation group (LCA preservation, n=40) and the control group (LCA non-preservation, n=45). The surgery-related indicators, postoperative recovery status, as well as gastrointestinal motility indicators, and anal function (Wexner constipation score) before and after surgery, were compared between the two groups. The recurrence and metastasis of the two groups were compared at 12 months of follow-up, and the postoperative complications were recorded. Results: The surgical time in the observation group was lengthened compared with that in the control group (P<0.05), and there were no statistical differences in the intraoperative blood loss, the total number of dissected lymph nodes, the number of dissected lymph nodes in group 253, ischemic changes of the stump during surgery and the number of prophylactic stomata between both groups (P>0.05). The postoperative first exhaust time and hospital stay were markedly shorter in the observation group compared to the control group (P<0.05), and there was no statistical significance in the fluid intake time (P>0.05). After surgery, the levels of serum MTL and GAS in the two groups were significantly reduced compared to before surgery (P<0.05) while the Wexner constipation score was significantly enhanced compared with before surgery (P<0.05), but there were no statistical significant differences in the changes of the above indicators before and after treatment between the two groups (P>0.05). During 12 months of follow-up, there was 1 case of local recurrence and 2 cases of liver metastasis in the observation group and 3 cases of local recurrence and 3 cases of liver metastasis in the control group, and the differences in the incidence rates of recurrence and metastasis were not statistically significant (P>0.05). During the 12-month follow-up, there were no statistical differences in the incidence rates of complications such as anastomotic leakage, anastomotic bleeding, and urinary tract infection between the two groups (P>0.05). Conclusion: LCA preservation during laparoscopic low anterior resection does not affect the gastrointestinal function and lymph node dissection and can shorten postoperative exhaust time and hospital stay, and it has the same efficacy in recurrence and metastasis as that without preservation of LCA. 2023 Vol. 29 (2): 302-306 [Abstract] ( 79 ) HTML (1 KB)  PDF (1208 KB)  ( 114 ) 307 Efficacy and Safety of Unilateral Lobectomy with Isthmectomy in the Treatment of T1/2cN0M0 Unilateral Multifocal Papillary Thyroid Carcinoma YAN Shide, HU Kaibing, HU Gang, et al DOI: 10.3969/j.issn.1006-6233.2023.02.025 Objective: To study the clinical efficacy and safety of unilateral lobectomy with isthmectomy in the treatment of T1/2cN0M0 unilateral multifocal papillary thyroid carcinoma (MPTC). Methods: Patients with T1/2cN0M0 unilateral MPTC who were admitted to the General Surgery Department, the Second People's Hospital of Hefei from January 2019 to December 2020 were selected as the research subjects. According to the inclusion and exclusion criteria, 51 patients who underwent total thyroidectomy combined with prophylactic bilateral VI lymph node dissection were selected as the control group. According to the gender, age±5, and tumor-node-metastasis (TNM) stage matching principle, 51 patients who underwent unilateral lobectomy combined with isthmectomy and prophylactic bilateral VI lymph node dissection in the hospital during the same period were selected as the observation group. The 2 groups were compared in terms of the operation time, intraoperative blood loss, length of the surgical incision, drainage volume, number of days of drainage tube removal, hospital stay, the incidence of postoperative complications, and postoperative progression-free survival (PFS). Results: The operation time, incision, intraoperative blood loss, postoperative drainage volume, number of days of drainage tube removal, and the hospital stay of the observation group were shorter/less than those of the control group (P<0.05). The incidence of postoperative complications in the observation group (11.76%) was significantly lower than that in the control group (49.02%) (P<0.05). No death or loss cases were found during follow-up. There were 2 cases of external cervical lymph node metastasis in the control group during the follow-up period, with an average survival time of 11 months and a PFS rate of 96.08%. There were 3 cases of recurrence and 2 cases of cervical lymph node metastasis in the observation group during the follow-up period, with an average survival time of 11 months and a PFS rate of 90.20%. The 2 groups had similar PFS (χ2=1.359, P=0.244). Conclusion: Unilateral lobectomy combined with isthmectomy is effective and safe in the treatment of T1/2cN0M0 unilateral MPTC, which can effectively reduce postoperative complications, thereby improving quality of life of the patients, and preventing recurrence. 2023 Vol. 29 (2): 307-311 [Abstract] ( 70 ) HTML (1 KB)  PDF (1321 KB)  ( 105 ) 311 Changes in Lipid Metabolism and Coagulation Function in Patients with Primary Chronic Kidney Disease and Their Clinical Significance LUO Jun, GENG Keming, ZHANG Yunhui, et al DOI: 10.3969/j.issn.1006-6233.2023.02.026 Objective: To study the changes and clinical significance of lipid metabolism and coagulation function in patients with primary chronic kidney disease (CKD). Methods: 120 patients with CKD admitted to the hospital from January 2020 to April 2022 were selected as samples for a cross-sectional study. After admission, blood lipid metabolism indicators such as blood urea nitrogen (BUN), serum creatinine (Scr), total cholesterol (TC), triacylglycerol (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apolipoprotein A1 (apoA1) and apoB as well as coagulation function indicators of fibrinogen (FIB), D-dimer (D-D), activated partial thromboplastin time (APTT), fibrinogen degradation product (FDP), thrombin time (TT), prothrombin time (PT) and international normalized ratio (INR) were measured. The patients were divided into the CVD group and the control group according to whether CVD occurred or not. The indicators of the two groups were compared and the Logistic regression model was established, and then the predictive value of the model on CVD risk was analyzed by calibration. Results: 34 cases (28.33%) of 120 patients with CKD were complicated with CVD. The proportion of patients with CKD stage 5 and levels of BUN, Scr, TC, LDL-C, and FIB in the CVD group were higher than those in the control group while the level of HDL-C was lower than that in the control group (P<0.05). CKD staging was negatively correlated with lipid metabolism indicators of HDL-C, apoA1, and apoB (P<0.05), and was positively correlated with coagulation function indicators of PT, FIB, D-D, and INR (P<0.05). Binary Logistic regression analysis showed that TC, HDL-C, and FIB were closely related to CVD in patients with CKD (P<0.05), and the predictive model for the risk of CVD in patients with CKD was shown as logit(P)= -2.864+ 0.571× (TC) -2.748× (HDL-C) +0.620× (FIB). The AUC, sensitivity, and specificity of predicting CVD in patients with CKD were 0.807 (95%CI: 0.725~0.873), 82.35%, and 69.77%. Conclusion: The lipid metabolism and coagulation function of patients with primary CKD are abnormal, and there is a significant correlation with CKD staging. The logistic regression model established based on TC, HDL-C, FIB and other indicators have a good reference value for predicting the risk of CVD in patients with CKD. 2023 Vol. 29 (2): 311-317 [Abstract] ( 86 ) HTML (1 KB)  PDF (2010 KB)  ( 147 ) 317 Effects of Unicompartmental Knee Arthroplasty and Total Knee Arthroplasty on Perioperative Indexes, Knee Function and Postoperative Complications in Patients with Medial Unicompartmental Knee Osteoarthritis LU Hao, WANG Peng, et al DOI: 10.3969/j.issn.1006-6233.2023.02.027 Objective: To explore the perioperative indexes, knee function, and postoperative complications of patients with medial unicompartmental knee osteoarthritis treated by unicompartmental knee arthroplasty (UKA) and total knee arthroplasty (TKA). Methods: The clinical data of 104 patients with medial unicompartmental knee osteoarthritis who were treated in the hospital from March 2020 to June 2021 were retrospectively analyzed. According to the surgical methods, 51 patients underwent unicompartmental knee arthroplasty and were made the UKA group, and the other 53 patients received total knee arthroplasty and were made the TKA group. The perioperative-related indexes of the two groups were compared, and the range of motion (ROM) of the knee joint and hospital for special surgery knee score (HSS) were evaluated and compared between the two groups before surgery and at 6 and 12 months after surgery. The lower limb alignment correction and postoperative complications were compared before and after treatment with the two surgical methods. Results: Compared with the TKA group, the surgical time and 90° voluntary knee flexion time in the UKA group were shorter, the intraoperative blood loss was less and the decrease of hemoglobin was less at 3 days after surgery (P<0.01). There were no significant differences in the ROM and HSS scores between the two groups before surgery (P>0.05), and the ROM and HSS scores at 6 and 12 months after surgery were significantly higher than those before surgery (P<0.01). The ROM of the UKA group at 6 and 12 months after surgery was significantly higher than that of the TKA group (P<0.01), and the HSS score at 6 months after surgery was significantly higher than that of the TKA group (P<0.05). After surgery, the hip-knee-ankle angle of the two groups was enlarged (P<0.01) while the tibiofemoral angle was reduced (P<0.01), and the lower limb alignment was corrected, but the differences in hip-knee-ankle angle and tibiofemoral angle were not significant between the two groups (P>0.05). The incidence rate of postoperative complications at 3.92% in the UKA group was significantly lower than the TKA group at 18.87% (P<0.05). Conclusion: Compared with total knee arthroplasty, unicompartmental knee arthroplasty for patients with medial unicompartmental knee osteoarthritis has a shorter time required for surgery and 90° voluntary knee flexion time, less intraoperative blood loss, better postoperative knee range of motion and joint function recovery and fewer postoperative complications, so unicompartmental knee arthroplasty is worthy of clinical promotion and application. 2023 Vol. 29 (2): 317-322 [Abstract] ( 58 ) HTML (1 KB)  PDF (1617 KB)  ( 114 ) 323 Effect of Osteochondral Grafting on Coagulation Function Gait Parameters and Lower Limb Force Lines after Osteochondral Injury of the Talus ZHAO Zhenshuan, LI Jun, YU Xiaoguang, et al DOI: 10.3969/j.issn.1006-6233.2023.02.028 Objective: To investigate the effect of osteochondral transplantation on lower limb alignment and ankle-knee joint function after talar osteochondral injury (OLTs). Methods: The clinical data of 104 patients with OLTs in our hospital from July 2018 to September 2020 were collected and analyzed retrospectively. According to the surgical plan, they were divided into the control group (n=52) and the observation group (n=52), who underwent microfracture surgery and osteochondral transplantation, respectively. The improvement degree of lower limb alignment, excellent and good rate of ankle joint recovery, complications, and coagulation function indexes [D-dimer (DD), fibrinogen (Fib)] before and after surgery were observed in the two groups, as well as the HSS joint scores, visual analog scale (VAS)scores, AOFAS scores, and gait parameters (normalized stride length, percentage of time supported by the affected foot, cadence). Results: The VAS score of the observation group was lower than that of the control group at 6 months and 12 months after surgery, and the HSS, AOFAS scores, standardized stride length, stride frequency, and the percentage of foot support time in the observation group were higher than those in the control group (P<0.05); The levels of DD and Fib in the two groups at 3 days after operation were higher than those before operation (P<0.05), and there was no significant difference in the levels of DD and Fib between the two groups at 3 days and 7 days after the operation (P>0.05); At 12 months after the operation, the improvement of lower limb alignment and the excellent and good rate of ankle joint recovery in the observation group were better than those in the control group (P<0.05); There was no significant difference in the incidence of complications between the two groups at 12 months after operation (P>0.05). Conclusion: Osteochondral grafting helps to improve lower limb force lines and gait parameters and restore ankle and knee function in patients with OLTs, with less impact on coagulation function. 2023 Vol. 29 (2): 323-328 [Abstract] ( 58 ) HTML (1 KB)  PDF (2186 KB)  ( 130 ) 328 Effect of Small Incision Combined with Kirschner Wire and Manual Reduction and Plaster External Fixation in the Treatment of Supracondylar Fracture of Humerus on Elbow Function in Children LIU Liedong, MA Jiangwei, BAI Xiaobing DOI: 10.3969/j.issn.1006-6233.2023.02.029 Objective: To investigate the effect of small lateral elbow incisions combined with lateral Kirschner wire and closed reduction combined with Kirschner wire on elbow joint function in children with supracondylar fracture of the humerus. Methods: Clinical data of 74 children with supracondylar fractures of the humerus admitted to the Department of Orthopedics of our hospital from July 2019 to July 2020 were retrospectively collected. They were divided into two groups based on the treatment method: a small lateral elbow incision combined with lateral Kirschner wire for the incised group (40 cases), and closed repositioning combined with Kirschner wire for the closed group (34 cases). The clinical efficacy of the two groups of children was obtained at 7 weeks postoperatively, and the elbow function scores (MEPS scores) of the two groups of children were collected before, at 3 weeks, at 5 weeks, and 7 weeks postoperatively, and the range of motion of the elbow joint before and after rotation and flexion and extension of the two groups of children were counted at 7 weeks postoperatively. Results: The hospital stay and fracture healing time of the closed group were shorter than those of the incised group (P<0.05). The clinical excellence rate in the closed group was 97.06%, significantly higher than the 80% in the incised group (P<0.05).The MEPS scores of the two groups showed an upward trend with the time change in 7 weeks after surgery, and the upward trend was stronger in the closed group. Elbow anterior rotation, posterior rotation, and flexion and extension were significantly higher in both groups at 7 weeks postoperatively (P<0.05); the difference between the closed group and each angle was significantly higher than that of the incised group at 7 weeks postoperatively (P<0.05). Conclusion: Lateral elbow incision combined with lateral Kirschner wire and closed reduction combined with Kirschner wire can treat supracondylar fracture of the humerus. Closed reduction combined with Kirschner wire can improve the clinical efficacy of supracondylar fracture of the humerus and improve elbow joint function. 2023 Vol. 29 (2): 328-332 [Abstract] ( 44 ) HTML (1 KB)  PDF (1325 KB)  ( 123 ) 332 Clinical Study on Postoperative Pain of Mixed Hemorrhoids with Methylene Blue Blocking Injection Combined with Ketochromic Acid Tromethamine GAO Yingjie, BI Wenjing, HAN Guochao, et al DOI: 10.3969/j.issn.1006-6233.2023.02.030 Objective: To investigate the efficacy of methylene blue blocking injection combined with keto chromic acid tromethamine in the treatment of postoperative pain of mixed hemorrhoids. Methods: A total of 80 cases of mixed hemorrhoids admitted from January 2020 to December 2021 were divided into an experimental group and a control group, with 40 cases in each group. As to the experimental group, after the operation, methylene blue long-acting analgesic (1% methylene blue 2mL+water for injection 5mL+0.375% ropivacaine 5mL)+ketochromic tromethamine 60mg intramuscular injection was injected into the wound sealing. As to the control group, keto chromic acid tromethamine 30~60mg intramuscular injection was used as required, with the daily dose<120mg. Results: The pain scores at 6h, 1d, 3d, and 7d after the operation were statistically different (P<0.001, P<0.05), and the pain scores at 14d after the operation were not statistically different (P=0.602, P>0.05), namely, the pain of the patients in the experimental group in the first two weeks was significantly less than that of the patients in the control group, and the incision pain of the patients in the two groups was not significantly different after two weeks. In the comparison between the two groups of patients under strong stimulation (defecation and dressing change), there was a statistical difference in pain scores P<0.001 (P<0.05), namely, the pain of patients in the experimental group during defecation and dressing change was significantly less than that of patients in the control group. Conclusion: Methylene blue blocking injection combined with keto chromic acid tromethamine can significantly reduce postoperative pain, improve postoperative quality of life, and reduce postoperative urinary retention. This scheme is a safe and effective treatment measure, which is worthy of clinical application. 2023 Vol. 29 (2): 332-336 [Abstract] ( 79 ) HTML (1 KB)  PDF (1208 KB)  ( 153 ) 336 Selecting Kilovolts to Reduce the Radiation Dose of CT-Guided Radiofrequency Ablation for Liver Tumors Based on BMI ZHAO Junlu, NIE Guanwei, LIU Chong, et al DOI: 10.3969/j.issn.1006-6233.2023.02.031 Objective: To explore the effect of selecting kilovolts based on body mass index (BMI) on reducing the radiation dose of radiofrequency ablation in liver tumors under CT guidance. Methods: CT-guided RFA for hepatic ablations performed from January 1, 2019, through December 31, 2019, were retrospectively reviewed (55 men and 45 women; aged, 29~90 years). The radiation dose was recorded during each of the following steps: planning, performing, and postprocedure. BMI-based protocol modification changes in tube voltage and tube current were then applied to hepatic ablations performed subsequently (52 men and 48 women; aged 30~91 years). Image quality and overall operator satisfaction were noted for each case (score, 1~5). Dose reduction after modification was then calculated. Results: Retrospective analysis found a mean (± SD) overall CT dose index (CTDI) for CT-guided RFA to be 12.83±3.78mGy. After protocol modification, the mean CTDI decreased to 3.84 ±2.24 mGy, a 70.07% reduction overall. The image quality was slightly lower compared to before the modification, but the image quality in both stages met the needs of CT-guided radiofrequency ablation for liver tumors. The difference between electrode needle display and operator confidence satisfaction was not statistically significant (P> 0.05). Conclusion: BMI-based kilovolt modifications can significantly reduce the radiation dose during CT-guided percutaneous liver tumor ablation without significantly sacrificing image quality. 2023 Vol. 29 (2): 336-339 [Abstract] ( 65 ) HTML (1 KB)  PDF (1402 KB)  ( 108 ) 339 Correlation between Microangiopathy and Retinal Vessel Diameter in Patients with Type 2 Diabetes Mellitus WANG Mei, JIANG Chao, et al DOI: 10.3969/j.issn.1006-6233.2023.02.032 Objective: To investigate the relationship between microangiopathopathy and retinal vessel diameter in type 2 diabetes mellitus (T2DM). Methods: A total of 213 patients with T2DM hospitalized in Chengde Central Hospital from 2018 to 2019 were collected. The retinal vessel diameter was measured semi-automatically according to fundus photography, and the central retinal artery equivalent value (CRAE), central retinal vein equivalent value (CRVE), and arteriovenous ratio (AVR) were calculated. Patients were divided into 4 groups according to different microangiopathopathy: 94 cases in the NC group, 69 cases in the DR group, 24 cases in the DN group, and 26 cases in the DNR group. The basic clinical data of the 4 groups of patients were compared, and the vascular differences among the non-proliferative DR patients at different stages were compared. Disordered multiple logistic regression was used to analyze the relationship between microangiopathopathy and retinal vessel diameter. Results: In the NC group, DR group, DN group, and DNR group, the CRVEs were (257.05±22.40)μm, (266.97±23.66)μm, (270.24±17.67)μm and (278.64±25.91)μm, respectively. The venous diameter increased gradually and the difference was statistically significant (P<0.001). AVRs were (0.69±0.06), (0.66±0.06), (0.65±0.06) and (0.64±0.08), respectively. The arteriovenous ratio decreased gradually and the difference was statistically significant (P<0.001). Among 210 patients with non-proliferative DR, there were 117 cases in the DR-free group, 39 cases in the DRⅠ group, 32 cases in the DRⅡ group, and 22 cases in the DRⅢ group. Spearman correlation analysis showed that CRVE was positively correlated with the non-proliferative DR stage (r=0.235, P=0.001), and AVR was negatively correlated with the non-proliferative DR stage (r=-0.217, P=0.002). Disordered multiple logistic regression analysis showed that CRVE and AVR were significantly correlated with microangiopathy. The increase in retinal vein diameter and decrease in the arteriovenous ratio are independent risk factors for diabetic microangiopathies. Conclusion: The increase in retinal vein diameter and the decrease in arteriovenous ratio predict the severity of systemic microcirculation in T2DM patients. 2023 Vol. 29 (2): 339-344 [Abstract] ( 59 ) HTML (1 KB)  PDF (1518 KB)  ( 132 ) 344 Effects of Sacubitril/Valsartan Sodium and Valsartan on Myocardial Enzymes, NT-proBNP and Inflammatory Factors in Elderly Patients with Coronary Heart Disease after PCI ZHANG Zutao, LIAO Wei, ZHANG Nan, et al DOI: 10.3969/j.issn.1006-6233.2023.02.033 Objective: To compare the effects of sacubitril/valsartan sodium and valsartan on myocardial enzymes, N-terminal pro-B-type natriuretic peptide (NT-proBNP), and inflammatory factors in elderly patients with coronary heart disease after percutaneous coronary intervention (PCI). Methods: The data of 139 elderly patients with coronary heart disease after PCI in our hospital from January 2020 to January 2022 were retrospectively collected, of which 40 patients received conventional western medicine treatment after PCI for coronary heart disease (control group), 50 treated with Shakubatrac valsartan sodium on the basis of routine treatment (Shakubatrac valsartan sodium group), and 49 treated with valsartan on the basis of routine treatment (valsartan group). The 3 groups were compared in terms of cardiac function, myocardial enzymes, NT-proBNP, and inflammatory factors. The incidence of major adverse cardiac events (MACE) was recorded. Results: The difference in left ventricular ejection fraction (LVEF), left ventricular end-systolic diameter (LVESD), and left ventricular end-diastolic diameter (LVEDD) before and after treatment in the sakubactrovalsartan sodium group were higher than those in valsartan group and the control group (P<0.05). The difference between creatine kinase (CK), creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST), NT-proBNP, tumor necrosis factor (TNF)-α and interleukin (IL)-6 before and after treatment in sakubactrovalsartan sodium group were higher than those in valsartan group and the control group (P<0.05). The total incidence of MACE in the sakubactrovalsartan sodium group, valsartan group, and control group were 6.00%, 14.29% and 27.50%, the difference between the 3 groups was statistically significant (P<0.05). Conclusion: For elderly patients with coronary heart disease after PCI, sacubitril/valsartan sodium treatment can improve cardiac function, restore myocardial enzymes, lower the levels of NT-proBNP and inflammatory factors, and reduce the risk of MACE after the operation. 2023 Vol. 29 (2): 344-349 [Abstract] ( 86 ) HTML (1 KB)  PDF (1225 KB)  ( 192 )

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