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2022 Vol. 28, No. 5 Published: 2022-05-31 705 Effects of Rapamycin on Proliferation Apoptosis and PD-1 / PD-L1 Signaling Pathway of JAK2 V617F Positive HEL Cells QI Lin, et al DOI: 10.3969/j.issn.1006-6233.2022.05.01 Objective: To explore the effect of Rapamycin (Rapa) on proliferation and apoptosis of JAK2 V617F positive HEL cells and the expression of programmed death receptor 1 and its ligand (PD-1/PD-L1). Methods: Human erythroleukemia HEL cells with JAK2 V617F mutation positive were cultured in vitro. Rapa with concentrations of 10nm, 50nm and 100nm were added respectively. The control group was established. The inhibition rate of cell proliferation was detected by CCK-8. Caspase 3 / 7 activity detection kit was used to detect the caspase 3/7 activity of cells in each group, Transwell chamber was used to observe the cell migration ability of each group, flow cytometry was used to detect the apoptosis and PD-1 / PD-L1 expression of cells in each group, real-time fluorescence quantitative polymerase chain reaction was used to detect the mRNA changes of JAK2, PD-1 and PD-L1 in cells in each group, and Western blot was used to detect the expression of mammalian rapamycin target protein (mTOR) in each group. Five healthy volunteers were selected. Their lymphocytes were isolated and co cultured with HEL cells. Different concentrations of Rapa were added. The number of Treg cells in each group was detected by flow cytometry. Results: Results of CCK-8 showed that:The inhibition rates of cell proliferation at 72h with different final concentrations of Rapa (10 nM, 50 nM, 100 nM) were (33.33±4.6)%, (49.12±3.72)%, (55.16±4.14)%(P<0.05), respectively. Rapa treated HEL cells at concentrations of 50nM and 100nM after 24h,Caspase 3/7 activity was significantly increased compared with control(P<0.05).The results of flow cytometry showed that the apoptotic rate increased significantly compared with the control.PD-L1 expression was significantly decreased compared with the control(P<0.05).The mRNA expression of JAK2 and PD-L1 was significantly downregulated compared with the control (P<0.01).Healthy volunteer lymphocytes co-cultured with HEL cells for 24h showed an increase in Treg cells(P<0.05).Rapa could reduce Treg cells in a dose-dependent manner.Western blot showed that Rapa was able to inhibit mTOR expression in a dose-dependent manner. Conclusion: Rapa may interfere with the JAK2 pathway by affecting mTOR, which leads to the inhibition of Hel cell proliferation, the reduction of PD-L1 expression, and the suppression of Treg cells. 2022 Vol. 28 (5): 705-710 [Abstract] ( 144 ) HTML (1 KB)  PDF (2285 KB)  ( 219 ) 711 Lncrna Cytor Targeting Wnt/ β- Study on the Mechanism of Catenin Pathway Promoting the Growth of Oral Squamous Cell Carcinoma MI Lei, MIAO Hui, XU Yang, et al DOI: 10.3969/j.issn.1006-6233.2022.05.02 Objective: To study the role of lncRNA CYTOR on the proliferation, migration and invasion in oral squamous cell carcinoma (OSCC) and its underlying molecular regulatory mechanisms. Methods: CAL27 cells at logarithmic phase were randomly divided into Control group (normal cultured cells), si-NC group (transfected with empty plasmid) and si-lncRNA CYTOR group (transfected with si-lncRNA CYTOR plasmid). RT-qPCR was used to detect the expression of lncRNA CYTOR in normal oral cells (NHOK) and OSCC cells (Tca8113, CAL27, SCC9 and SCC25). Cell proliferation was detected by CKK-8, clone formation and EdU staining assays, cell migration and invasion were detected by Transwell assay, and the expression of cell proliferation-related proteins (PNCA and Ki-67) and Wnt/β-catenin pathway-related proteins (c-myc, cyclin D1 and β-catenin) were detected by Western blot. Results: Compared with NHOK cells, the expression of lncRNA CYTOR in OSCC cells (Tca8113, CAL27, SCC9 and SCC25) was significantly decreased, especially in CAL27 cells (P<0.01). The evaluation of cell function in vitro showed that compared with si-NC group, cell activity, clone formation ability, the proportion of EdU positive cells, migration and invasion in si-lncRNA CYTOR group were significantly reduced (P<0.01). In addition, compared with si-NC group, the expression levels of PCNA, Ki-67, c-myc, cyclin D1 and β-catenin in si-lncRNA CYTOR group were significantly decreased (P<0.01). Conclusion: LncRNA CYTOR is highly expressed in OSCC, and lncRNA CYTOR silencing could suppress the proliferation, migration and invasion of CAL27 cells, and its mechanism may be related to the inhibition of Wnt/β-catenin pathway. 2022 Vol. 28 (5): 711-714 [Abstract] ( 169 ) HTML (1 KB)  PDF (1604 KB)  ( 226 ) 715 Effects of Dexmedetomidine-Regulated MiR-132-3p on Cerebral Microvascular Cell Injury Induced by Hypoxia and Reoxygenation WANG Shaowei, XING Zhen, et al DOI: 10.3969/j.issn.1006-6233.2022.05.03 Objective: To study the effect of dexmedetomidine (Dex) regulateing miR-132-3p on the damage of cerebral microvascular cells (HBMEC) induced by hypoxia and reoxygenation (H/R). Methods: Human HBMEC were cultured in vitro and intervened with low, medium and high doses (0.01, 0.10, 1.00 μmol/L) of Dex to establish H/R injury models. The miR-NC or miR-132-3p mimics were transfected into HBMEC to establish H/R injury models. After HBMEC were transfected with anti-miR-NC or anti-miR-132-3p, they were intervened with 1.00 μmol/L Dex, and then H/R injury models was established. The levels of IL-1β, IL-6 and TNF-α were detected by ELISA; the levels of MDA, SOD and CAT were detected by the kit; the expression of miR-132-3p was detected by RT-qPCR. Results: After HBMEC was induced by H/R, the levels of IL-1β, IL-6, TNF-α and MDA were increased (P<0.05), but the levels of SOD and CAT were decreased (P<0.05), and the expression level of miR-132-3p was decreased (P<0.05). After Dex intervention, the levels of IL-1β, IL-6 TNF-α and MDA in H/R-induced HBMEC decreased (P<0.05), but the levels of SOD and CAT increased (P<0.05), and the expression level of miR-132-3p increased (P<0.05). After up-regulation of miR-132-3p, the levels of IL-1β, IL-6 TNF-α and MDA in H/R-induced HBMEC decreased(P<0.05), but the levels of SOD and CAT increased (P<0.05). Down-regulation of miR-132-3p reversed the effects of Dex on H/R-induced inflammatory factor expression and oxidative stress in HBMECs. Conclusion: Dex could inhibit H/R-induced injury of human brain microvascular endothelial cells by up-regulating miR-132-3p. 2022 Vol. 28 (5): 715-719 [Abstract] ( 86 ) HTML (1 KB)  PDF (1215 KB)  ( 126 ) 720 The Role of MIF in the Progression of Cigarette Smoke Induced Inflammation in Rats with COPD ZHONG Li, LI Tianhao, WANG Huiqin DOI: 10.3969/j.issn.1006-6233.2022.05.04 Objective: To investigate the role of macrophage mobile inhibitors in the inflammatory development of chronic obstructive pulmonary disease (COPD) rats. Methods: SD male rats were randomly divided into COPD models (n=20), ISO-1 (n=20), and healthy controls (n=20). The rat COPD model was prepared by using cigarette smoking alone, cigarette smoke mold was given to the COPD group and rats in ISO-1 were intraperitoneally injected with MIF inhibitor (ISO-1) one hour before each smoke at 0~30d,60~90d. Five individuals in each group were killed at 30d,60d and 90d, respectively, by HE staining for lung tissue inflammation, MIF (macrophage mobility inhibitory factor) and IL-6 levels in rat serum and lung tissue homogenates, and MIF protein expression was determined by immunohistochemical staining. Results: MIF was significantly highly expressed in serum and lung tissues in the COPD model group, but low in the ISO-1 group and statistically significant (P<0.05), while both rat serum and lung tissues were higher than in healthy controls (P<0.05). Lung tissue immunohistochemistry also showed higher MIF expression (except 30th d) in each time period model group than in the ISO-1 group as well as healthy controls, while the ISO-1 group was only higher than the healthy controls (P<0.05). The associated inflammatory factor, IL-6, is closely related to MIF, IL-6 expression in the ISO-1 group was significantly decreased in rat serum and lung tissue than in the model group. High expression was observed only in the unsuppressed model group (P<0.05). Lung histopathology showed a significant reduction in inflammation in group ISO-1. The COPD maturity was significantly reduced compared to the model group, showing that blocking MIF attenuated COPD inflammation in rats. Conclusion: MIF is significantly expressed in the development of COPD and is closely associated with inflammatory status, indicating that it plays an important role in the development of COPD inflammation, which can further reflect the inflammatory status of COPD and accelerate COPD disease progression, while inhibition of MIF can improve the inflammatory status of COPD rats, which provides a new idea for the treatment of clinical COPD patients. 2022 Vol. 28 (5): 720-724 [Abstract] ( 74 ) HTML (1 KB)  PDF (2023 KB)  ( 126 ) 725 Emodin Inhibits Proliferation Migration and Invasion of Pancreatic Cancer SW1990 Cells by Up-regulating miR-1301 MA Liying, et al DOI: 10.3969/j.issn.1006-6233.2022.05.05 Objective: To explore the roles of emodin on proliferation, migration and invasion of pancreatic cancer SW1990 cells and its molecular mechanism. Methods: Human pancreatic ductal epithelial cells HPDE6 and pancreatic cancer SW1990 cells were cultured in vitro, and SW1990 cells were divided into control group, low-dose emodin group, medium-dose emodin group, high-dose emodin group, miR-NC group, miR-1301 group, high-dose emodin+anti-miR-NC group, high-dose emodin+anti-miR-1301 group. Real-time fluorescence quantitative PCR (RT-qPCR) was used to detect the expressions of miR-1301. The tetramethylazozolate colorimetric method (MTT) was implemented to measure cell activity. The protein expression was assessed by Western blot. Transwell was employed to monitor cell migration and invasion. Results: Compared with human pancreatic ductal epithelial cells HPDE6, the expression level of miR-1301 in pancreatic cancer SW1990 cells was significantly decreased(P<0.05). Compared with the control group, medium and high-doses of emodin significantly decreased the activity, migration and invasion ability and the levels of CyclinD1, MMP-2 and MMP-9, and increased the level of p21 of pancreatic cancer SW1990 cells(P<0.05). Compared with the control group, the expression levels of miR-1301 in medium and high-dose emodin groups were significantly increased(P<0.05). Overexpression of miR-1301 significantly reduced the activity, migration and invasion ability and the levels of CyclinD1, MMP-2 and MMP-9, and increased the level of p21 of pancreatic cancer SW1990 cells(P<0.05). Down-regulation of miR-1301 reversed the roles of emodin on proliferation, migration and invasion of pancreatic cancer SW1990 cells(P<0.05). Conclusion: Emodin may inhibit cell proliferation, migration and invasion by up-regulating the expression of miR-1301 in SW1990 cells. 2022 Vol. 28 (5): 725-730 [Abstract] ( 81 ) HTML (1 KB)  PDF (2000 KB)  ( 114 ) 731 Inhibitory Effect of Recombinant Mutant Human TRAIL in Combination with 5-FU on Transplanted Tumor of Colorectal Cancer in Nude Mice SUN Tongyou, ZHAO Lixia, et al DOI: 10.3969/j.issn.1006-6233.2022.05.06 Objective: To observe the antitumor effect of recombinant mutant human tumor necrosis factor (TNF)-related apoptosis-inducing ligand (rmhTRAIL) or known as circular permuted TRAIL (CPT) in combination with 5-fluorouracil (5-FU) on transplanted tumor of colorectal cancer in nude mice. Methods: Human colorectal cancer (CRC) cell line HCT116 was inoculated subcutaneously into the middle lateral axillary of each nude mouse to establish transplanted tumor model in nude mice. After randomization, 5-FU was injected into caudal vein and CPT was injected into abdominal cavity. The general situation of nude mice was observed, and the weight and tumor length and diameter of nude mice were measured. Finally, all the animals were sacrificed, and then the tumor tissues were stripped, photographed and weighed. The tumor cell apoptosis was determined by Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)/2 4',6-diamidino-2-phenylindole (DAPI) assay, and observed and photographed by Laser confocal microscope. Results: CRC bearing nude mice model was successfully established, and the nude mice were all in good condition. CPT and 5-FU alone could inhibit the growth of transplanted tumor in nude mice, and the comparison of that with control group has statistical difference (P<0.01). The combination of the two drugs had more significant inhibitory effect on the growth of transplanted tumor in nude mice, and the high-dose CPT group was the most significant (P<0.01). In comparison with control group, the tumor volume and weight of CPT, 5-FU and co-treatment groups were significantly reduced (P<0.05). CPT, 5-FU and their combination promoted the apoptosis of tumor cells, especially in the combination group, and the difference was significant in comparison with control (P<0.05). Conclusion: CPT alone or in combination with 5-FU can significantly suppress the growth of human CRC HCT116 transplanted tumors in nude mice, and promote the apoptosis of tumor cells CRC tumor-bearing nude mice. 2022 Vol. 28 (5): 731-735 [Abstract] ( 132 ) HTML (1 KB)  PDF (1870 KB)  ( 181 ) 736 The Effect of General Anesthesia with Tracheal Intubation Versus General Anesthesia with Laryngeal Mask in Patients Undergoing Laparoscopic Cholecystectomy WU Lan, et al DOI: 10.3969/j.issn.1006-6233.2022.05.07 Objective: To study the effect of general anesthesia with laryngeal mask versus general anesthesia with tracheal intubation in patients with laparoscopic cholecystectomy and the influence on hemodynamics. Methods: A total of 60 patients undergoing laparoscopic cholecystectomy in our hospital were selected and randomly divided into a laryngeal mask general anesthesia group and an tracheal intubation general anesthesia group, 30 cases in each.The heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP), blood oxygen saturation (SpO2), electroencephalographic bispectral index (BIS), and the dose of atracurium and isoproterenol were observed at the time points before induction of anesthesia (T0), before laryngeal mask and tracheal intubation placement after induction (T1), at the time of laryngeal mask and tracheal intubation placement (T2), at the time of laparoscopic placement (T3), at 30 min into the operation (T4), and when the preoperative modified sedation and wakefulness score (OAA/S) was ≥4 (T5) in the two groups. The doses of atracurium and isoproterenol were also observed, as well as the patient's response and post-anesthesia wakefulness time during removal of the mask and tracheal intubation in both groups. Results: The satisfaction rate of anesthesia in the laryngeal mask group was 90.00%, which was significantly higher than that in the tracheal intubation group, which was 73.33% (χ2=9.815, P<0.05). The doses of propofol and atracurium in the laryngeal mask group were significantly lower than those in the tracheal intubation group (t=2.177, 4.162, P<0.05); the awakening time of the patients in the laryngeal mask group was significantly shorter than that in the tracheal intubation group, and the incidence of somatic choking reaction was significantly lower than that in the tracheal intubation group (t/χ2=3.175, 6.812 , P<0.05); the levels of SBP, DBP and HR in the laryngeal mask group at time points T2 and T4 were significantly lower than those in the tracheal intubation group (P<0.05), but there was no significant difference in the BIS values between the two groups at each time point (P>0.05). Conclusion: The use of laryngeal mask general anesthesia in laparoscopic cholecystectomy can effectively reduce the dosage of anesthetic drugs, and the patient's blood flow is more stable than that of tracheal intubation patients under general anesthesia, and the patients wake up quickly after surgery. It is worthy of clinical application. 2022 Vol. 28 (5): 736-739 [Abstract] ( 153 ) HTML (1 KB)  PDF (1195 KB)  ( 151 ) 740 Correlation between Platelet Lymphocyte Ratio and DiseaseActivity of Aystemic Lupus Erythematosus GU Yanqin, MA Haozhe, CONG Shan, et al DOI: 10.3969/j.issn.1006-6233.2022.05.08 Objective: To explore the correlation between platelet to lymphocyte ratio (PLR) and disease activity in patients with systemic lupus erythematosus(SLE)during the first episode and initial treatment. Methods: A total of 572 patients with first attack and initial treatment of SLE were enrolled as SLE group, and 572 healthy people during the same period were enrolled as healthy control group, according to SLE disease activity index 2000, SLE patients were divided into low activity group (SLEDAI ≤ 9 points, n=294) and high activity group (SLEDAI > 9 points, n=278), the PLR of the above groups were compared respectively, and spearman statistical analysis was performed to investigate the correlation between PLR and disease activity of SLE. Results: The PLR of SLE patients was significantly higher than that of healthy controls (P<0.05). In SLE patients, the PLR of the high activity group was significantly higher than that of the low activity group (P<0.05). PLR was positively correlated with SLEDAI score (r=0.291, P<0.05), Erythrocyte sedimentation rate(r=0.207, P<0.05), C-reactive protein (r=0.302, P<0.05), anti-ds-DNA antibody(r=0.098, P<0.05),24h urine protein (r=0.173, P<0.05), negatively correlated with complement C3(r=-0.257, P<0.05) and complement C4(r=-0.223, P<0.05). The area under the receiver operating characteristic curve of PLR for predicting the high activity of SLE is 0.639 (P<0.05). When the optimal critical value was 170, the sensitivity was 58.3% and the specificity was 65.6%. Conclusion: PLR can be used as one of the evaluation indicators for disease activity of SLE. 2022 Vol. 28 (5): 740-744 [Abstract] ( 104 ) HTML (1 KB)  PDF (1297 KB)  ( 138 ) 745 Expression Levels of X-Chromosome-Linked Zinc Finger Protein and Axin in Gastric Cancer Tissues and Correlation with Clinicopathological Factors ZHENG Wei, et al DOI: 10.3969/j.issn.1006-6233.2022.05.09 Objective: To analyze the expression levels of X-chromosome-linked zinc finger protein and axin expression levels in gastric cancer tissues and their correlation with clinicopathological factors. Methods: From Feb. 2018 to Feb. 2021, 300 patients with gastric cancer in our hospital were retrospectively selected, and 300 cases of their gastric cancer tissues and 140 cases of their paracancerous tissues (more than 5.0 cm away from the gastric cancer tissues) were surgically excised down, and the expression of ZFX and Axin in gastric cancer tissues and paracancerous tissues were statistically analyzed, and the correlation between the expression of ZFX and Axin in gastric cancer tissues and clinicopathological factors was analyzed. Results: The positive rate of ZFX expression in gastric cancer tissues was 84.33% (253/300) higher than that in paraneoplastic tissues 17.14% (24/140), and the negative rate was 15.67% (47/300) lower than that in paraneoplastic tissues 82.86% (116/140) (P<0.05); the positive rate of Axin expression was 34.00% (102/300) lower than that in paraneoplastic tissues 84.29% (118/140) and the negative rate was 66.00% (198/300) higher than that of 15.71% (22/140) in paraneoplastic tissues (P<0.05). The positive rate of ZFX expression in gastric cancer tissues of patients with Duke stage I-II was lower than that of patients with stage III-IV (P<0.05), the positive rate of ZFX expression in gastric cancer tissues of patients with low, medium and high differentiation gradually decreased (P<0.05), the positive rate of ZFX expression in gastric cancer tissues of patients with infiltration depth of outer mucosal layer, mucosal layer, mucosa and submucosa gradually decreased (P<0.05). The positive rate of ZFX expression in gastric cancer tissues of patients with lymph node metastasis was higher than that of patients without lymph node metastasis (P<0.05). The positive rate of Axin expression in gastric cancer tissues of patients with Duke stage I-II was higher than that of patients with stage III-IV (P<0.05). The positive rate of Axin expression in gastric cancer tissues of patients with low, medium and high differentiation gradually increased (P<0.05). The positive rate of Axin expression in gastric cancer tissues of patients with lymph node metastasis was lower than that of patients without lymph node metastasis (P<0.05). Conclusion: The positive expression rate of X-linked zinc finger protein and axin in gastric cancer tissues is increased and decreased, which are related to Duke stage, degree of differentiation, depth of invasion and lymph node metastasis in clinicopathological factors. 2022 Vol. 28 (5): 745-749 [Abstract] ( 72 ) HTML (1 KB)  PDF (1202 KB)  ( 127 ) 750 The Relationship Between XRCC1 TYMS MTHFR Gene Polymorphisms and the Efficacy of Adjuvant Chemotherapy After Colon Cancer Surgery ZHAO Shuai, CHEN Jing, YAN Haiyang, et al DOI: 10.3969/j.issn.1006-6233.2022.05.010 Objective: To investigate and analyse the efficacy of adjuvant chemotherapy with FOLFOX4 after surgery for stage II and III colon cancer, and to analyse the relationship between X ray repair cross complementary gene I (XRCC1), TYMS, methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms and efficacy, with the aim of finding and analysing the predictors of adjuvant chemotherapy. Methods: The clinical data of 104 patients who received 6 cycles of FOLFOX4 adjuvant chemotherapy after the end of surgical treatment admitted to our hospital from May 2018 to January 2020 were retrospectively analyzed, and the 2-year disease-free survival rate was judged at follow-up after treatment and used as an efficacy evaluation criterion. DNA was extracted from tumour tissue specimens of all patients, and XRCC1, TYMS and MTHFR genotypes were tested. A chi-square test was used to compare the recurrence rates between genotypes, followed by a COX regression model to explore factors that might have an impact on the efficacy. Results: The genotype distribution of XRCC1, TYMS and MTHFR was dominated by G/A genotype, 2R/3R genotype and T/C genotype respectively, which were significantly higher than the other genotypes, and the difference was statistically significant (P<0.05). The difference was statistically significant (P<0.05). The XRCC1 gene polymorphism had a direct effect on the disease-free survival rate at 2 years after FOLFOX4 adjuvant chemotherapy for colon cancer (P<0.05). The results of the univariate analysis of the COX model showed that TNM stage, intestinal wall infiltration, XRCC1 and adjuvant chemotherapy with FOLFOX4 after surgery for colon cancer were correlated (P<0.05). Conclusion: The XRCC1 gene polymorphism has a direct correlation with disease free survival at 2 years after FOLFOX4 adjuvant chemotherapy for colon cancer.Detection of XRCC1 polymorphism can be used as a reliable predictor of the efficacy of adjuvant chemotherapy with FOLFOX4 after surgery for stage II and III colon cancer. 2022 Vol. 28 (5): 750-753 [Abstract] ( 100 ) HTML (1 KB)  PDF (1185 KB)  ( 158 ) 754 Expression and Correlation Studies of β2-MG MiR-182 BUN in Elderly Chronic Renal Failure SUN Yao, SUN Kun DOI: 10.3969/j.issn.1006-6233.2022.05.011 Objective: To study the expression and correlation of β2 microglobulin (β2-microglobulin, β2-MG), microRNA-182 (miR-182), and blood ureanitrogen (BUN) in elderly chronic renal failure. Methods: Totally 43 patients with chronic renal failure treated from March 2019 to December 2020 were selected as the study group; 43 normal subjects who underwent physical examination in the same period were selected as the control group. Serum miR-182 expression was detected by fluorescence quantitative polymerase chain reaction technique, β2-MG and BUN levels were detected by immunoturbidimetric method, serum CYSC, Scr and RBP levels were detected by enzyme-linked immunosorbent assay, and subject operator characteristics curve (ROC) was used to analyze the diagnostic value of β2-MG, miR-182 and BUN in chronic renal failure in the elderly. Results: The expression levels of β2-MG, miR-182, and BUN in the study group were higher than those in the control group (P<0.05). Correlation analysis showed a positive correlation between β2-MG and miR-182 (r=0.695, P=0.001); between β2-MG and BUN (r=0.733, P=0.001); and between miR-182 and BUN (r=0.778, P=0.001) (P<0.05). The levels of β2-MG, miR-182 and BUN were higher in early stage patients than in non-early stage patients in the study group (P<0.05). The expression levels of β2-MG, miR-182 and BUN were higher in elderly patients with chronic renal failure aged ≥65 years, with oliguria, oedema and large amounts of urine protein (P<0.05). ROC analysis of β2-MG, miR-182 and BUN was less sensitive than the three combined in diagnosing elderly chronic renal failure (P<0.05). Conclusion: β2-MG, miR-182, and BUN are highly expressed in elderly chronic renal failure, and there is a correlation between the three, which has certain clinical value in the diagnosis of elderly chronic renal failure. 2022 Vol. 28 (5): 754-758 [Abstract] ( 76 ) HTML (1 KB)  PDF (1371 KB)  ( 89 ) 759 Changes in the Levels of Eosinophil Chemokines and Inflammatory Mediators During Acute Exacerbations of Chronic Bronchitis in the Elderly and Their Clinical Significance XIE Mingying, YANG Yuanrui, CAI Lingqin, et al DOI: 10.3969/j.issn.1006-6233.2022.05.012 Objective: To explore the changes and clinical significance of eosinophil chemokines and inflammatory mediators in elderly patients with acute attack of chronic bronchitis. Methods: A total of 186 elderly patients with chronic bronchitis (study group) and 80 healthy elderly people (control group) were selected between August 2020 and September 2021. The levels of eosinophil chemokines and inflammatory mediators in the two groups were tested and compared. Results:The levels of eosinophil chemokines and monocyte chemotactic protein-1 (MCP-1) in study group were higher than those in the control group (P<0.05). The levels of tumor necrosis factor-α (TNF-α), high-sensitivity C-reactive protein (hs-CRP) and procalcitonin (PCT) in study group were higher than those in the control group (P<0.05). The levels of eotaxin and MCP-1 in elderly patients with acute attack of chronic bronchitis were higher than those in chronic protracted stage and remission stage (P<0.05). The levels of eosinophil chemokines and MCP-1 in chronic protracted stage were higher than those in remission stage (P<0.05). The levels of TNF-α, hs-CRP and PCT in elderly patients with acute attack of chronic bronchitis were higher than those in chronic protracted stage and remission stage (P<0.05). Conclusion: The detection of Eotaxin, MCP-1, TNF-α, hs-CRP and PCT has important indications in elderly patients with acute attack of chronic bronchitis. 2022 Vol. 28 (5): 759-762 [Abstract] ( 77 ) HTML (1 KB)  PDF (1191 KB)  ( 186 ) 763 Correlation of Serum BNP and CRP with the Severity of Myocardial Injury and Cardiac Function in Elderly Patients with Coronary Heart Disease and Heart Failure YANG Liping, MA Junjie, HOU Bin, et al DOI: 10.3969/j.issn.1006-6233.2022.05.013 Objective: To analyze the correlation of serum brain natriuretic peptide (BNP), and C-reactive protein (CRP) with the severity of myocardial injury and cardiac function in elderly patients with coronary heart disease and heart failure. Methods: A total of 106 elderly patients with coronary heart disease and heart failure admitted to Suining Central Hospital were selected between January 2019 and January 2021. The levels of serum BNP, cardiac troponin I (cTnI) and CRP were detected, and correlation analysis was conducted. Results: Serum BNP, CRP and cTnI levels of the patients enrolled were (578.09±46.17) g/ml, (7.07±1.85) mg/l and (0.050±0.011) ng/ml. Correlation analysis showed that serum BNP and CRP levels were positively correlated with cTnI (P<0.05). There were statistically significant differences in serum BNP and CRP levels between patients with different grades of heart failure (P<0.05). Serum BNP and CRP levels of patients with gradeⅠ~Ⅱ heart failure were significantly lower than those of patients with grade Ⅲ and grade Ⅳ (P<0.05). Serum BNP and CRP levels of patients with grade Ⅲ heart failure were significantly lower than those of patients with grade IV (P<0.05). Serum BNP, cTnI and CRP levels were positively correlated with cardiac function classification (P<0.05). Conclusion:Serum BNP, cTnI and CRP levels are correlated with heart failure in elderly patients with coronary heart disease, when serum BNP, cTnI, and CRP levels are significantly increased, it is necessary to be alert to the aggravation of myocardial injury and the increase of cardiac function. 2022 Vol. 28 (5): 763-765 [Abstract] ( 80 ) HTML (1 KB)  PDF (1214 KB)  ( 140 ) 766 Expression and Significance of NF-κB Protein and Apaf-1 Protein in Gastric Cancer YANG Bo, YANG Tao, YANG Dongxia, et al DOI: 10.3969/j.issn.1006-6233.2022.05.014 Objective: To explore the expression and significance of nuclear factor-κB (NF-κB) and apoptotic protease activating factor-1 (Apaf-1) in gastric cancer tissues, and to open up new ideas for the clinical treatment of gastric cancer. Methods: A total of 50 experimental specimens were collected, including gastric cancer tissues and corresponding paracancerous tissues in the same period. The expressions of NF-κB and Apaf-1 proteins were detected by immunohistochemistry SP method, and the relationship between the two and clinical pathological characteristics as well as their correlation were analyzed. Results: The expression level of NF-κB protein in gastric cancer tissues was higher than that of adjacent tissues, while the expression level of Apaf-1 protein was lower than that of adjacent tissues. The protein expression changes of the two proteins were related to the differentiation degree, TNM stage, infiltration depth and lymphatic metastasis (P<0.05). Correlation analysis showed that the expressions of NF-κB protein and Apaf-1 protein in gastric cancer tissues were negatively correlated (rs=-0.36,P<0.05). Conclusion: The abnormal expression of NF-κB and Apaf-1 proteins in gastric cancer tissues may be involved in the occurrence and development of gastric cancer. 2022 Vol. 28 (5): 766-769 [Abstract] ( 84 ) HTML (1 KB)  PDF (1506 KB)  ( 105 ) 770 Clinical Analysis of Bone Marrow Cell Morphology and Immunophenotype of Patients with Multiple Myeloma ZENG Yiling, WEI Xiaohong, et al DOI: 10.3969/j.issn.1006-6233.2022.05.015 Objective: To investigate the bone marrow cell morphology and immunophenotype of patients with multiple myeloma (MM). Methods: A total of 116 patients with MM admitted to the hospital were selected as the observation group between July 2018 and June 2021. Meanwhile, 86 patients with hyperplastic anemia treated in the hospital were selected as the control group. The bone marrow cell morphology was observed under a light microscope, and the immunophenotype was detected by four-color flow cytometry. Results: In the observation group, there were 112 (96.55%) patients with active bone marrow hyperplasia and 4 (3.45%) patients with reduced bone marrow hyperplasia. Bone marrow smears of patients with MM showed different sizes and shapes of proplasmacytes. The proportions of different subtypes of immunophenotyping in observation group were significantly higher than those in the control group (P<0.05), and the proportions of CD38+ , CD138+ and CD56+ were the highest(P<0.05).There was no significant difference in the proportion of immunophenotype between patients with different proportions of myeloma cell subgroups in the observation group (P>0.05),and the proportions of the first three were the highest. There was no significant difference in above-mentioned indicators among MM patients with different proportions of myeloma cells, different protein types, different clinical stages, and patients with and without bone destruction (P>0.05).Conclusion: CD38+, CD138+, and CD56+ are specific immune proteins in myeloma cells of patients with MM. Combination with morphological analysis and immunophenotyping can provide an objective basis for the diagnosis of MM. 2022 Vol. 28 (5): 770-774 [Abstract] ( 126 ) HTML (1 KB)  PDF (1206 KB)  ( 431 ) 775 Effect of Preoperative Sleep quality on Women's Hyperalgesia after Cesarean Section HAN Xue, JIN Ling, SUN Hong, et al DOI: 10.3969/j.issn.1006-6233.2022.05.016 Objective: To analyze the effect of preoperative sleep quality on women's hyperalgesia after cesarean section. Methods: 586 cesarean section women who came to our hospital for treatment from November 2019 to November 2020 were studied. According to the score of digital pain scale (NRS) on the 5th day after operation, they were divided into hyperalgesia group (n = 152, NRs ≥ 4) and control group (n = 434, NRS < 4). The related factors of postoperative hyperalgesia in women undergoing cesarean section were analyzed by univariate and logistic multivariate analysis. Results: Compared with the control group, the proportion of preoperative sleep disturbance, spinal anesthesia, general anesthesia, intraoperative fluid volume, and midazolam use in the hyperalgesia group were higher, and the operation time and postoperative exhaust time were higher. postoperative urination time, postoperative defecation time were longer, the difference was significant (P<0.05). Logistic multivariate analysis showed that preoperative sleep disturbance, spinal anesthesia and general anesthesia, operation time ≥ 50min, and postoperative exhaust time ≥ 20h were risk factors for postoperative hyperalgesia in women with cesarean section (P<0.05). Conclusion: Preoperative sleep disturbance is a risk factor for postoperative hyperalgesia in women undergoing cesarean section. 2022 Vol. 28 (5): 775-778 [Abstract] ( 106 ) HTML (1 KB)  PDF (1180 KB)  ( 134 ) 779 Effects of YAG Laser on Antioxidant Function Cosmetic Result and Skin Barrier Function in Patients with Chloasma YAN Xiaoxue, WANG Huanhuan DOI: 10.3969/j.issn.1006-6233.2022.05.017 Objective: To study the effect and safety of large spot and low-energy Nd:YAG laser in the treatment of chloasma. Methods: Totally 125 patients with chloasma admitted to the hospital were selected between January 2018 and January 2021, and randomly divide into observation group (63 cases) and control group (62 cases) using random number table method. Patients in the control group were treated with hydroquinone cream, and patients in the observation group were treated with large spot low-energy Nd:YAG laser based on the treatment of control group. Therapeutic effects, antioxidant capacity, cosmetic results and skin barrier function in the two groups were compared. Results: After treatment, the total response rate in the observation group was significantly higher than that in the control group (P<0.05). The levels of malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) in the two groups showed no statistically significant difference before and after treatment, and there was no statistically significant difference in the difference value of each indicator before and after treatment (P>0.05). After treatment, MDA was significantly reduced (P<0.05), while SOD and CAT were significantly increased in the two groups (P<0.05). After treatment, skin melanin index (MI) and erythema index (EI) were significantly reduced in the two groups (P<0.05). The difference values of MI and EI before and after treatment in observation group were significantly higher than those in the control group (P<0.05). After treatment, the sebum content and water content of stratum corneum were significantly reduced (P<0.05), while transepidermal water loss (TEWL) was significantly increased in the two groups (P<0.05). There was no significant difference between the two groups in the difference values of sebum content, water content of stratum corneum and TEWL before and after treatment (P>0.05). Conclusion: The large spot low energy Nd:YAG laser combined with hydroquinone cream has better therapeutic and cosmetic skin results than hydroquinone cream only treatment for melasma, and it is safer and does not damage the patient's antioxidant capacity and skin barrier function. 2022 Vol. 28 (5): 779-783 [Abstract] ( 65 ) HTML (1 KB)  PDF (1644 KB)  ( 253 ) 784 The Effects of Ropivacaine on Inflammatory Response and PKA/CREB Pathway in Rats with Osteoarthritis Pain LIN Yumei, FU Mingjun, CHEN Jisheng DOI: 10.3969/j.issn.1006-6233.2022.05.018 Objective: To investigate the effects of ropivacaine on the inflammatory response and protein kinase A (PKA)/cAMP response element binding protein (CREB) pathway in rats with osteoarthritis (OA) pain. Methods: Rats were randomly divided into sham operation group, OA group (modeling) and low, medium, and high dose ropivacaine groups (modeling + intraperitoneal injection of 0.5, 1, 2 mg/kg ropivacaine), 16 in each group; the drug was administered after 2 weeks of modeling by cut off ligamenta cruciata, for two weeks. The tenderness threshold of rats was measured before model replication, 2 weeks after model replication, and 2 weeks after administration. Enzyme-linked immunoassay (ELISA) method was used to detect serum IL-1β, IL-6, and TNF-α levels, real-time fluorescent quantitative PCR method to determine the expression levels of IL-1β, IL-6, TNF-α, PKA, CREB mRNA in cartilage tissue, Western Blot to determine the levels of p-PKA/PKA and p-CREB/CREB in cartilage tissue, hematoxylin-eosin staining to observe cartilage tissue lesions.Results: After 2 weeks of administration, compared with the sham operation group, the tenderness threshold of rats in the OA group was significantly reduced (P<0.05), the serum IL-1β, IL-6, TNF-α levels, and cartilage tissue IL-1β, IL-6, TNF-α, PKA, CREB mRNA expression levels, p-PKA/PKA, p-CREB/CREB protein expression levels were significantly increased (P<0.05), the cartilage tissue had obvious pathological phenomena; compared with the OA group, the tenderness threshold of rats in the low, medium and high dose ropivacaine groups was significantly increased (P<0.05), the serum IL-1β, IL-6, TNF-α levels, and cartilage tissue IL-1β, IL-6, TNF-α, PKA, CREB mRNA expression levels, p-PKA/PKA, p-CREB/CREB protein expression levels were significantly reduced (P<0.05), and in a dose-dependent manner, the cartilage tissue lesions were alleviated.Conclusion: Ropivacaine can inhibit the activation of PKA/CREB pathway in rats with OA pain, reduce inflammation in the body, and reduce the degree of knee joint pain. 2022 Vol. 28 (5): 784-788 [Abstract] ( 146 ) HTML (1 KB)  PDF (1520 KB)  ( 281 ) 789 Relationship between Type 2 Diabetic Nephropathy and Serum Netrin-1 Level LI Jingjing, et al DOI: 10.3969/j.issn.1006-6233.2022.05.019 Objective: To analyze the relationship between type 2 diabetic nephropathy and serum Netrin-1 level, and to provide a new idea for risk prediction of type 2 diabetic nephropathy. Methods: 180 patients with type 2 diabetes admitted to our hospital from August 2019 to August 2021 were divided into normal albuminuria group (UAER < 30 mg/gCr), microalbuminuria group (UAER 30~<300 mg/gCr) and massive albuminuria group (UAER≥300 mg/gCr) according to UAER. The age, body mass index (BMI) and serum Netrin levels of the three groups were compared, and Logistic multivariate regression analysis was used to summarize the related factors affecting UAER in patients with type 2 diabetes mellitus. Results: Logistic regression analysis showed that the course of disease ≥5 years, systolic blood pressure ≥120 mmHg, diastolic blood pressure ≥90mmHg, FPG≥6.1 mmol/L and Netrin-1≥300 ng/L were independent risk factors for UAER in patients with type 2 diabetes mellitus (P<0.05). Serum Netrin-1 in normal albuminuria group, microalbuminuria group and massive albuminuria group increased in turn, and the difference between the groups was statistically significant (P<0.05). Pearson correlation analysis showed that serum Netrin-1 was positively correlated with UAER (r=0.616, P<0.05). Conclusion: The increase of serum Netrin-1 is one of the important factors in the occurrence and development of diabetic nephropathy in patients with type 2 diabetes mellitus, and the increase of Netrin-1 is closely related to the increase of UAER. 2022 Vol. 28 (5): 789-792 [Abstract] ( 95 ) HTML (1 KB)  PDF (1247 KB)  ( 112 ) 793 Plasma Level of Adrenocorticotropic Hormone and Clinical Significance in Type 2 Diabetes Mellitus Patients with Obesity KANG Juge, DANG Qian DOI: 10.3969/j.issn.1006-6233.2022.05.020 Objective: To investigate the Plasma adrenocorticotropic hormone (ACTH) level and clinical significance of type 2 diabetes mellitus (T2DM) patients with obesity. Methods: 102 T2DM patients with obesity treated in our hospital from January 2019 to June 2021 were selected as the research object and classified as the obesity group. At the same time, 100 T2DM patients without obesity treated in our hospital in the same period were selected as the non-obesity group, and 95 healthy adults who underwent physical examination in our hospital in the same period were selected as the control group. Fasting blood glucose (FPG), fasting insulin (fins), triglycerides (TG), total cholesterol (TC), homeostasis model insulin resistance index (HOMA-IR), plasma ACTH level and bone mineral density (BMD) were compared among the three groups. Pearson correlation analysis and multiple linear regression were used to explore the correlation between plasma ACTH level and various indexes in T2DM patients with obesity. Results: BMI, FPG, FINS, HOMA-IR, TG, TC and ACTH in obese group were significantly higher than those in the other two groups, and BMD was significantly lower than those in the other two groups. BMI, FPG, FINS, HOMA-IR, TG, TC and ACTH in non-obese group were significantly higher than those in the control group, and BMD was significantly lower than that in the control group (P<0.05). Pearson correlation analysis showed that the plasma ACTH level in obese patients was significantly positively correlated with BMI, FPG, FINS, HOMA-IR, TG and TC, and negatively correlated with BMD (P<0.05). Multiple linear regressions showed that BMI, FPG, HOMA-IR and BMD were the independent influencing factors of plasma ACTH level in obese patients (P<0.05). Conclusion: The plasma ACTH level of T2DM patients with obesity significantly increased, which was related to the disorder of glucose and lipid metabolism and the decrease of bone mineral density. 2022 Vol. 28 (5): 793-796 [Abstract] ( 108 ) HTML (1 KB)  PDF (1196 KB)  ( 210 ) 797 Effects of Hemodialysis and Peritoneal Dialysis on Macular Blood Flow Density and Morphology in Patients with Non-diabetic Nephropathy CHENG Quan, et al DOI: 10.3969/j.issn.1006-6233.2022.05.021 Objective: To explore the effects of hemodialysis and peritoneal dialysis on macular blood flow density and morphology in patients with non-diabetic nephropathy (NDN). Methods: A total of 132 patients with NDN who needed to be treated with dialysis and admitted to the hospital between January 2020 and March 2021 were selected. According to the dialysis modality, they were divided into hemodialysis group (65 cases) and peritoneal dialysis group (67 cases). The levels of hemoglobin (Hb), serum albumin (SAB), serum creatinine (Scr), blood urea nitrogen (BUN), interleukin-6 (IL-6) and high-sensitivity C-reactive protein (hs-CRP) were detected and compared between the two groups before and after dialysis. The range of 6 mm×6 mm macular area was analyzed by optical coherence tomography angiography (OCTA). The blood flow density of retinal surface, middle and deep capillary plexus, blood flow density of FD300, area and circumference of central macular foveal avascular zone (FAZ), A-circularity index (AI), and the occurrence of complications in the two groups were examined. Results: ①There was no statistically significant difference in Hb, SAB, Scr, and BUN between the two groups before dialysis (P>0.05). After dialysis, Hb and SAB in the two groups were higher than those before dialysis (P<0.05), Scr and BUN were lower than those before dialysis (P<0.05). The differences of Hb and SAB before and after dialysis in the peritoneal dialysis group were lower than those in the hemodialysis group (P>0.05). The differences of Scr and BUN were higher than those in the hemodialysis group (P<0.05). ②There was no statistically significant difference in IL-6 and hs-CRP between the two groups before dialysis (P>0.05). After dialysis, IL-6 and hs-CRP in the two groups were lower than those before dialysis (P<0.05), and the differences in peritoneal dialysis group were lower than those in the hemodialysis group (P<0.05). ③There was no statistically significant difference in blood flow densities of the inner retina surface layer, middle layer, deep layer and FD300 between the two groups before dialysis (P<0.05). After dialysis, the blood flow densities in the two groups were higher than those before dialysis (P<0.05), and the differences in peritoneal dialysis group were lower than those in the hemodialysis group (P<0.05). ④There was no statistically significant difference in area and circumference of FAZ, and AI between the two groups before dialysis (P>0.05). The area and circumference of FAZ, and AI in the hemodialysis group showed no statistically significant difference before and after dialysis (P>0.05). After dialysis, the circumference of FAZ in the peritoneal dialysis group was smaller than that before dialysis (P<0.05), and AI was higher than that before dialysis (P<0.05). The differences of area and circumference of FAZ, and AI before and after dialysis in the peritoneal dialysis group were higher than those in the hemodialysis group (P<0.05). ⑤The incidence of fever in peritoneal dialysis group was significantly lower than that in hemodialysis group (P<0.05). However, there was no significant difference in the incidence rates of hypotension, hypoglycemia, peritonitis and cerebral edema, and fatality rate between the two groups (P>0.05). Conclusion: For patients with non-diabetic nephropathy, both hemodialysis and peritoneal dialysis can effectively improve their nutritional status. Compared with hemodialysis, peritoneal dialysis can better improve renal function and reduce blood density of retinal middle and deep layers. However, it induces more inflammatory factorsand increases AI. Clinically, the dialysis plan should be determined after comprehensive consideration. 2022 Vol. 28 (5): 797-802 [Abstract] ( 83 ) HTML (1 KB)  PDF (1224 KB)  ( 177 ) 803 Clinical Characteristics and Cognitive Impairment of Benign Epilepsy in Children with Central Temporal Spikes ZHANG Tiantian, LENG Xuerong, QI Xiaohong, et al DOI: 10.3969/j.issn.1006-6233.2022.05.022 Objective: To study the clinical characteristics,EEG features and cognitive impairment of benign epilepsy in children with central temporal spikes (BECT) . Method: A total of 105 newly diagnosed BECT children were selected as the experimental group, and 105 healthy children were matched as the control group. The clinical course, MRI data and EEG were collected and analyzed in BECT group. Cognitive function was assessed between the two groups using the Wechsler Intelligence Scale for Children--4th edition (WISC-IV). Result: BECT mostly attacked shortly after falling asleep or just before waking up. In this study, 45 BECT children presented with focal seizure (originating from the oropharynx) and the other 60 with secondary generalized tonic-clonic seizure(GTCS). And EEG showed bilateral discharge in 65 patients and unilateral discharge in 40 patients. Among 105 patients ,7 had mild abnormality in MRI and 20 had positive family history of epilepsy or febrile convulsion. The full-scale IQ (FSIQ) and verbal comprehension index (VCI), perceptual reasoning index(PRI), working memory index(WMI), and processing speed index (PSI) were significantly reduced in the BECT group (P<0.001) compared with the control group. In the BECT group, the FSIQ,VCI, WMI and PSI of patients with bilateral discharge was significantly lower than that with unilateral discharge (P=0.024, P<0.001,P=0.004,P=0.011), while there was no significant difference in PRI between the two groups (P>0.05). In terms of seizure form, seizure frequency and seizure duration respectively, the VCI of children with secondary GTCS was significantly lower than that of children with focal seizure (P=0.031); the VCI of children with high seizure frequency was significantly lower than that of children with low seizure frequency (P<0.001); the WMI of the children with long duration of attack was significantly lower than that of the children with short duration of attack (P=0.006); but there was no corresponding significant difference in FISQ and other indexes(all P>0.05). Conclusion: Children with BECT have characteristic clinical features and a lower full-scale IQ than normal children, whose verbal comprehension, perceptual reasoning, working memory and processing speed were impaired. Bilateral discharge in EEG may be a risk factor for cognitive impairment. The form, frequency and duration of seizures also affect cognitive impairment in different degrees. 2022 Vol. 28 (5): 803-808 [Abstract] ( 151 ) HTML (1 KB)  PDF (1228 KB)  ( 346 ) 809 Application Value of Modified PEWS Score in the Assessment and Prognosis of Children in Pediatric Emergencies ZHENG Xin, LIN Yanxun, CHEN Yue, et al DOI: 10.3969/j.issn.1006-6233.2022.05.023 Objective: To explore the application value of modified pediatric early warning score (PEWS) in the assessment and prognosis of children in pediatric emergencies. Methods: A total of 126 critically ill children admitted to the Department of Paediatrics of Huizhou Central People's Hospital in Guangdong Province from January 2021 to December 2021 were selected to be included in the observation group, and another 126 critically ill children admitted to the Department of Paediatrics of our hospital from January 2020 to December 2020 were selected to be included in the control group. In the control group, the PEWS score was used to assess the children's condition on admission, while in the observation group, the modified PEWS score was used to assess the children's condition on admission, and the children in the observation group were followed up for 6 months to record their survival. The prognosis of children with different modified PEWS scores in paediatric emergencies was compared, and the value of the modified PEWS scoring system in assessing condition and prognosis was analysed by using Spearman's rank correlation. Results: The early disease recognition rate and disease observation recognition rate of children in the observation group were 15.07% and 91.27%, which were higher than those of the control group (3.17% and 72.22%) (P<0.05); Spearman's rank correlation analysis showed a negative correlation between the modified PEWS score and disease classification (rs=-0.642, P<0.001). The AUC of the modified PEWS score on the prognosis of children with acute disease was 0.782 (P<0.05) in the ROC analysis. The AUC for predicting the condition of children with acute illness was 0.782 (P<0.05); 27 (21.43%) of the children in the observation group died at 6 months of follow-up, and there was a statistically significant difference (P<0.05) when comparing the mortality rates of children with different modified PEWS scores; ROC analysis showed that the AUC of the modified PEWS score for predicting death in children with paediatric emergencies was 0.775 (P<0.05). Conclusion: Modified PEWS score has high value in the assessment and prognosis of children in paediatric emergencies. 2022 Vol. 28 (5): 809-812 [Abstract] ( 175 ) HTML (1 KB)  PDF (1398 KB)  ( 314 ) 813 Coagulation Function Indicators in Premature Infants of Different Gestational Ages and Their Relationship with the Risk of Intracranial Hemorrhage ZHANG Jiao, WU Qi, et al DOI: 10.3969/j.issn.1006-6233.2022.05.024 Objective: To investigate the differences in coagulation function among premature infants of different gestational ages, and to analyze the risk factors of intracranial hemorrhage. Methods: The clinical data of 103 premature infants and their mothers hospitalized between January 2018 and January 2021 were retrospectively analyzed. According to gestational age, the infants enrolled were divided into group A (32~33 weeks) and group B (34~36 weeks). Coagulation function indicators were compared between the two groups. The correlation between coagulation function and gestational age, and the risk of intracranial hemorrhage were analyzed. Results: There was no significant difference in gender, age, gestational hypertension, diabetes, cesarean section rate, premature rupture of membranes rate and placental abruption rate between the two groups (P>0.05). The birth weight and gestational age of group A were significantly lower than those of group B (P<0.05). The prothrombin time (PT), activated partial thromboplastin time (APTT) and D-dimer (D-D) levels in group A were significantly longer/higher than those in group B (P<0.05), and fibrinogen (FIB) level was significantly lower than that in group B (P<0.05). Correlation analysis found that APPT (r=-0.247, P<0.05) and PT (r=-0.403, P<0.05) were negatively correlated with gestational age, and FIB was positively correlated with gestational age (r=0.227, P<0.05). D-D (r=-0.178, P>0.05) were not related to gestational age of premature infants. There were 10 cases of intracranial hemorrhage in group A, with an incidence of 23.26%, and 8 cases of intracranial hemorrhage in group B, with an incidence of 13.33%, indicating no significant difference between the two groups (χ2=1.710, P=0.191). Premature infants with and without intracranial hemorrhage were included in the hemorrhage group and the non-hemorrhage group, respectively. There were statistically significant differences in birth weight, gestational age, APTT and FIB between the two groups (P<0.05). Birth weight, APTT and FIB were independent risk factors for intracranial hemorrhage (P<0.05), with variance inflation factor (VIF)<3. Conclusion: APTT, FIB and D-D are significantly different in premature infants of different gestational ages, and APTT and FIB are related to gestational age. Low birth weight, short APTT and high FIB level may increase the risk of intracranial hemorrhage in premature infants. 2022 Vol. 28 (5): 813-817 [Abstract] ( 104 ) HTML (1 KB)  PDF (1256 KB)  ( 175 ) 818 Ablation Efficacy and Influencing Factors of Radioactive 131 Iodine in Postoperative Patients with Differentiated Thyroid Cancer JIA Yunxiang, HE Xingming, LU Wanjun, et al DOI: 10.3969/j.issn.1006-6233.2022.05.025 Objective: To explore thyroid ablation efficacy and influencing factors of postoperative radioactive 131 iodine (131I) in patients with differentiated thyroid carcinoma (DTC). Methods: 86 patients with thyroid cancer treated in our hospital from January 2019 to March 2020 were selected and divided into successful and unsuccessful ablation efficacy groups according to the effect of ablation efficacy. The differences of gender, age, pathological type, body mass index, TSH level and STG level before 131I first treatment, primary risk stratification, primary lesion diameter, TNM stage, metastasis and 131I first treatment dose between the two groups were analyzed by single factor statistics, Multivariate logistic regression was used to analyze the influencing factors of 131I nail cleaning efficacy in patients with DTC. Results: Among the 86 patients with DTC, 52 cases had successful ablation efficacy, and the successful rate was 60.47%. Univariate analysis showed that there were significant differences in STG level, recurrence risk stratification, primary lesion diameter and 131I first treatment dose between the successful group and the unsuccessful group (P<0.05); There was no significant difference in gender, age, pathological type, body mass index, TSH level, TNM stage and lymph node metastasis before 131I treatment (P>0.05). Multivariate logistic regression analysis showed that the level of STG before the first treatment of 131I, the diameter of primary lesion and the first treatment dose of 131I were the independent influencing factors of 131I ablation efficacy in patients with DTC (P<0.05). Conclusion: The success rate of thyroid ablation is 60.47% in the 86 patients with DTC. The sTg level before the first 131I treatment, diameter of primary lesions and dosage of the first 131I treatment are independent influencing factors of thyroid ablation efficacy of 131I on DTC patients. 2022 Vol. 28 (5): 818-822 [Abstract] ( 102 ) HTML (1 KB)  PDF (1205 KB)  ( 173 ) 823 Application of CT-guided Hook-wire Localization Combined with Thoracoscopic Surgery in Patients with Early Lung Cancer KONG Jinsong DOI: 10.3969/j.issn.1006-6233.2022.05.026 Objective: To explore the application value of CT-guided Hook-wire localization combined with thoracoscopic surgery in patients with early stage lung cancer. Methods: 60 patients with early-stage lung cancer from January 2018 to January 2021 were selected and grouped according to the random number table method, with 30 patients in each group. The control group was treated with conventional thoracoscopic surgery, and the study group was treated with CT guided hook wire positioning combined with thoracoscopic surgery. The surgical conditions, positive rate of incision margin, trauma stress indexes [interleukin-6 (IL-6), interleukin-8 (IL-8), cortisol (COR), C-reactive protein (CRP)], cardiopulmonary function [stroke volume (SV), left ventricular ejection fraction (LVEF), forced vital capacity (FVC), maximum ventilation volume per minute (MVV)] were compared between the two groups The levels of tumor related growth factors [vascular endothelial growth factor-C (VEGF-C), tumor specific growth factor (TSGF), epidermal growth factor receptor (EGFR)], the quality of life scale for lung cancer patients (FACT-L) and the incidence of complications. Results: Compared with the control group (161.55 ± 40.31) ml, (157.22 ± 35.21) min, (4.53 ± 1.26) d, (15.27 ± 4.02) d, (6.90 ± 0.46), the study group had less intraoperative bleeding (108.67 ± 26.83) ml, operation time (136.43 ± 30.19) min, thoracic drainage time (2.82 ± 0.90) d, hospital stay (11.34 ± 2.58) d, and more lymph node dissections (7.45 ± 0.50) (P<0.05). There was no significant difference in the positive rate of cutting edge between the two groups (P>0.05); Compared with the control group, the levels of serum IL-6, IL-8, cor and CRP in the study group were lower on the 1st, 3rd and 5th day after operation (P<0.05); SV, LVEF, FVC and MVV in the two groups 1 week and 1 month after operation were higher than those before operation, and compared with the control group, the study group was higher, the serum VEGF-C, TSGF and EGFR were lower than those before operation, and compared with the control group, the study group was lower (P<0.05); Three months after operation, the scores of physiological status, social / family status, relationship with doctors, emotional status, functional status and additional attention of the two groups were higher than those before operation, and the study group was higher than that of the control group (P<0.05); Compared with 26.67% (8 / 30) of the control group, the incidence of complications in the study group was 6.67% (2 / 30) (P<0.05). Conclusion: CT guided hook wire positioning combined with thoracoscopic surgery in the treatment of patients with early lung cancer can significantly optimize the operation, inhibit tumor growth, avoid positive margin, promote the recovery of cardiopulmonary function, reduce complications and improve the quality of life. 2022 Vol. 28 (5): 823-828 [Abstract] ( 92 ) HTML (1 KB)  PDF (1248 KB)  ( 235 ) 829 Efficacy of Arthroscopic Peripatellar Release Combined with Peripatellar Denervation in the Treatment of Anterior Knee Pain in Patellofemoral Arthritis and Its Effect on the Degree of Cartilage Degeneration WU Peng, JI Zhenwei, FENG Chongyang, et al DOI: 10.3969/j.issn.1006-6233.2022.05.027 Objective: To study the efficacy of arthroscopic peripatellar release combined with peripatellar denervation in the treatment of patellofemoral arthritis (PFOA) with anterior knee pain and its effect on the degree of cartilage degeneration. Methods: A total of 155 patients with PFOA and anterior knee pain were divided into study group (78 cases) and control group (77 cases) by the simple random grouping method. Both groups were treated with arthroscopic debridement, the control group with denervation and the study group with peripatellar release combined with peripatellar denervation, and they were followed up for 6 months after surgery. The efficacy, cartilage degeneration degree, knee function and adverse reactions of the two groups were recorded and compared. Results: Postoperatively, VAS scores were lower in both groups at all times compared to preoperatively, with time, group and interaction effects, and the study group was better than the control group (P<0.05); postoperatively, Lysholm knee function (LKSS) scores improved and the scores of Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) were significantly lower in both groups compared to preoperatively (P<0.05), and the study group was significantly better than the control group (P<0.05), and the differences of the above scores before and after surgery in study group were higher than those in control group (P<0.05). After treatment, the expression of matrix metalloproteinase-3 (MMP-3) was significantly decreased while the expression of tissue inhibitor of metalloproteinase-1 (TIMP-1) was significantly increased, and the expressions were significantly better in study group than those in control group, and the differences of the above indicators before and after surgery were higher in study group compared with those in control group (P<0.05). The number of postoperative complications in the study group was slightly lower than that in the control group, with incidence rates of 8.97% and 12.98% respectively, with no significant difference (P>0.05). Conclusion: Arthroscopic peripatellar release combined with peripatellar denervation in patients with PFOA and anterior knee pain can prompt the early recovery of knee motor function, reduce pain and slow down the degree of cartilage degeneration, with clinical promotion value. 2022 Vol. 28 (5): 829-833 [Abstract] ( 102 ) HTML (1 KB)  PDF (1207 KB)  ( 315 ) 834 Relationship between the Levels of Osteoprotegerin and Osteosclerosis Protein and the Degree of Coronary Artery Calcification in Maintenance Dialysis Patients DENG Zhenzhen, CHEN Cheng, XIONG Shuzhen, et al DOI: 10.3969/j.issn.1006-6233.2022.05.028 Objective: To explore the correlation between serum osteoprotegerin(OPG)or sclerostin(SO)level and coronary artery calcification(CAC)occurrence as well as related score(CAC score,CACS)in maintenance dialysis patients. Methods: 183 patients who received maintenance dialysis treatment in our department from November 2018 to November 2020 were selected as the research objects. Those with CACS ≤ 100 were included in the low group (80 cases) and those with CACS > 100 were included in the high group (103 cases). The clinical data of patients were collected and the levels of serum OPG and so were detected. Univariate analysis and multiple linear regression were used to analyze the independent risk factors of CAC in maintenance dialysis patients, and the correlation between OPG and so levels and CACS score was analyzed. Results: The age of dialysis and the number of diabetes in high group were higher than those in low group (P<0.05). The levels of serum CRP, OPG and so in high group were higher than those in low group (P<0.05); OPG level (r = 0.871, P = 0.000) and so level (r = 0.772, P = 0.000) were positively correlated with CACS score; Dialysis age, OPG, so and CRP levels were independent risk factors for coronary artery calcification in maintenance dialysis patients (P<0.05). Conclusion: The levels of serum OPG and so in maintenance dialysis patients are related to the occurrence of CAC. They may become a reliable means to predict CAC in the future. 2022 Vol. 28 (5): 834-838 [Abstract] ( 111 ) HTML (1 KB)  PDF (1244 KB)  ( 107 ) 839 Study on the Efficacy of Microdissection of 57 Cases of Auditory Neuroma and Analysis of Factors Influencing Postoperative Facial Nerve Function WANG Yan, WANG Qiang, NIE Er, et al DOI: 10.3969/j.issn.1006-6233.2022.05.029 Objective:To investigate the method and surgical effect of micro-resection of acoustic neuroma, and the influencing factors of facial nerve function after operation. Methods:The clinical data of 57 patients with acoustic neuroma admitted to the neurosurgery department of Affiliated Hospital of Xuzhou Medical University from August 2018 to October 2020 were retrospectively analyzed. The indexes that may be correlated with postoperative facial nerve dysfunction, such as operation time, postoperative infection, cerebrospinal fluid leakage and so on, were collected. Suboccipital retrosigmoid sinus approach was used for craniotomy, and neuroelectrophysiological monitoring was used during the operation to summarize the extent of tumor resection and the outcome of the disease. The above patients were followed up and evaluated at discharge and 3 months after operation, and the risk factors affecting facial nerve function were discussed according to House-Brackmann classification. Results:The total resection rate of acoustic neuroma was 91.22%, the postoperative infection rate was 7.02%, the hearing improvement rate was 87.72%, and the retention rate of acoustic nerve was 94.74%. 31 cases (54.39%) had normal facial nerve function at discharge, including 14 cases (24.56%) of House-Brackmann grade I and 17 cases (29.82%) of grade II; there were 26 cases of facial nerve function injury, including 11 cases of House-Brackmann grade III (19.30%) and 15 cases of grade IV~VI (26.32%). Three months after operation, 40 cases (70.18%) had normal facial nerve function, including 22 cases (38.60%) of House-Brackmann grade I and 18 cases (31.58%) of grade II; there were 17 cases of facial nerve function injury, including 10 cases of House-Brackmann grade III (17.54%) and 7 cases of grade IV-VI (12.28%). Conclusion:Tumor size, cystic change and peritumoral edema were independent risk factors for facial nerve dysfunction after operation (P=0.043,0.010,0.022). The larger the tumour, the presence of cystic degeneration and the more pronounced the peri-tumoural oedema, the greater the likelihood of post-operative facial nerve functional impairment. 2022 Vol. 28 (5): 839-842 [Abstract] ( 98 ) HTML (1 KB)  PDF (1568 KB)  ( 124 ) 843 Application of Advanced Audio-visual Dispersion Intervention in PPH LIU Xiaoli, CHEN Fengping, GAO Yingjie, et al DOI: 10.3969/j.issn.1006-6233.2022.05.030 Objective: To study the clinical effect of advanced audio-visual dispersion on vasovagal reflex (VVR) in hemorrhoidectomy (PPH). Methods: 100 patients with HHP from September 2017 to September 2019 were randomly divided into two groups.Both groups were given atropine and VVR during the operation, and the experimental group was intervened by advanced audio-visual dispersion. The preventive effect of the two groups on intraoperative VVR was compared. Results: VVR occurred in 2% (1 / 50) of the experimental group, which was lower than 16% (8 / 50) of the control group. The comparison results showed that the difference was statistically significant (P<0.05). The average heart rate (HR), arterial pressure (map) and other data of the two groups 30 minutes before operation were recorded. The level comparison results showed that there was no significant difference (P>0.05). The levels of map and HR in the control group were significantly lower than those before operation (P<0.05), and the decrease was greater than that in the experimental group (P<0.05). There was no significant difference in map and HR levels between the two groups 30 minutes after operation (P>0.05). Conclusion: Advanced audio-visual dispersion technology can reduce the VVR in the perioperative period of PPH patients. 2022 Vol. 28 (5): 843-846 [Abstract] ( 80 ) HTML (1 KB)  PDF (1339 KB)  ( 154 ) 847 Significance of Exhaled Nitric Oxide in Clinical Diagnosis and Treatment of Subacute Cough CHU Hui, ZHANG Jie DOI: 10.3969/j.issn.1006-6233.2022.05.031 Objective: To investigate the clinical value of exhaled nitric oxide (FeNO) in the diagnosis and treatment of subacute cough. Methods: 171 patients with subacute cough in respiratory medicine department of our hospital were selected to detect exhaled nitric oxide, and the results were analyzed. Results: The mean FeNO value of 171 patients was 36.89 ± 12.35, of which 47.95% were normal, 18.13% were intermediate and 33.92% were high. Among the 171 patients, there were 62 patients with pic, with an average FeNO value of 23.05±15.03. 83.87% of the patients were normal, 14.52% were intermediate and 1.61% were high; There were 58 patients with CVA, the mean FeNO value was 78.36 ± 22.67, of which 1.72% were normal, 8.62% were intermediate, and 89.66% were high; There were 36 patients with UACS, with an average FeNO value of 16.76 ± 7.88, of which 63.89% were normal, 33.33% were intermediate and 2.78% were high; There were 10 patients with EB, with an average FeNO value of 42.75 ± 13.72, of which 10.00% were normal, 50.00% were intermediate and 40.00% were high; There were 5 other patients with an average FeNO value of 15.38 ± 6.62, of which 100.00% were normal, 0.00% were intermediate and 0.00% were high. Compared with PIC, UACS, EB and other groups, the average FeNO value of CVA group was P<0.05, which was statistically significant. Compared with PIC, UACS and other groups, EB group had statistical significance (P<0.05). Compared with UACS and other groups, PIC group had no statistical significance (P>0.05). Compared with UACS and other groups, UACS had no statistical significance (P>0.05). 68 patients in CVA and EB groups were effective after treatment with inhaled hormone budesonide. Among them, 25 patients with CVA (43.10%) improved, 33 patients were cured (56.90%), and the effective rate was 100%. In EB group, 6 cases (43.10%) were improved, 4 cases (40.00%) were cured, and the effective rate was 100%. The total effective rate of the two groups was 100%. The level of FeNO in the two groups decreased significantly, which was significantly different from that before treatment. Conclusion: The detection of exhaled nitric oxide is of great value in the clinical diagnosis and treatment of subacute cough. It is of great significance for the diagnosis of cough variant asthma and eosinophilic bronchitis, and for the treatment of subacute cough. 2022 Vol. 28 (5): 847-852 [Abstract] ( 91 ) HTML (1 KB)  PDF (1324 KB)  ( 275 ) 853 Diagnostic Value of the Change of Serum PCT SAA CRP and ALB in Pancreatic Fistula after Surgery for Pancreatic Cancer CHEN Guoli, YI Haizhao, WANG Jianli DOI: 10.3969/j.issn.1006-6233.2022.05.032 Objective: To study the diagnostic value of the change of serum PCT, SAA, CRP and ALB in pancreatic fistula after surgery for pancreatic cancer. Methods: 83 patients who underwent pancreatic cancer surgery from January 2015 to January 2021 in Affiliated Hospital of Chengde Medical College were divided into pancreatic fistula group (21 cases, group A) and non pancreatic fistula group (62 cases, group B) according to whether pancreatic fistula occurred. The levels of PCT, SAA, CRP and ALB in the blood of the two groups were measured respectively. The blood was drawn and compared on the first day before operation and the second, fourth and seventh days after operation. Results: No significant difference was found between the patients one day before operationin two groups on serum levels of PCT, SAA, CRP and ALB(P>0.05) . But the patients two day after operation,four days after operation and seven days after operation in the group of pancreatic fistula on serum levels of PCT, SAA, CRP were higher than those of non- pancreatic fistula group, and on the levels of ALB was lower than those of non- pancreatic fistula group,the difference had statistically significant (P<0.05). Conclusion: When pancreatic cancer complicated with pancreatic fistula, serum PCT, SAA and CRP increased significantly, and ALB decreased significantly. This indicates that serum PCT, SAA, CRP and ALB have important diagnostic value for pancreatic fistula after pancreatic cancer surgery. 2022 Vol. 28 (5): 853-856 [Abstract] ( 173 ) HTML (1 KB)  PDF (1189 KB)  ( 281 ) 857 Analysis of the Efficacy and Risk of Delayed Bleeding in the Resection of Colorectal Polyps <1cm in Diameter with an Endoscopic Cold Snare XU Yanli, LU Qifeng, WANG Shuangping, et al DOI: 10.3969/j.issn.1006-6233.2022.05.033 Objective: To explore the efficacy of endoscopic cold snare resection of colorectal polyps with a diameter<1 cm and the risk factors for delayed bleeding. Methods: The data of 410 patients with colorectal polyps with a diameter<1cm who underwent endoscopic resection from September 2018 to February 2020 were retrospectively analyzed, and according to the different resection methods, they were divided into cold snare resection group (n=214 cases) and hot snare resection group (n=196 cases). The polyp resection time, complete polyp resection rate, polyp recovery rate were compared, complication occurrence of the two groups of patients and the occurrence of delayed bleeding were counted, and the clinical characteristics of bleeding patients and non-bleeding patients were compared. Logistic regression analysis was used to determine the factors affecting delayed bleeding in patients with colorectal polyps with a diameter<1 cm under endoscopic resection. Results: There were no statistically significant difference in the basic data of the two groups of patients (P>0.05). The time of polyp removal in the cold snare resection group was shorter than that in the hot snare resection group (P<0.05), and the incidence of delayed bleeding in the cold snare resection group was lower than that in the hot snare resection group (P<0.05) , there were no statistically significant difference in the resection rate of intact polyps, the recovery rate of polyps, the incidence of abdominal discomfort, and the incidence of perforation between the two groups (P>0.05). Compared with patients without bleeding, there were no statistically significant difference in gender, diabetes, coronary heart disease, hypertension, cerebrovascular disease, polyp number, use of titanium clamps, polyp pathological type, anticoagulant medication history, age, and polyp diameter (P>0.05), the polyp position, intraoperative bleeding, polyp morphology, and composition ratio of the resection method in bleeding patients were compared with those in non-bleeding patients, and the differences were statistically significant (P<0.05). Logistic multivariate regression analysis showed that polyp location, polyp morphology, and resection method are independent risk factors for delayed bleeding in patients with colorectal polyps with a diameter<1cm under endoscopic resection (P<0.05). Conclusion: Endoscopic cold snare resection for patients with colorectal polyps with diameter<1cm can shorten the time of polyp resection and reduce the incidence of delayed bleeding. In addition, the location of polyps, polyp morphology, and resection methods are independent risk factors that affect delayed bleeding in patients with colorectal polyps with a diameter diameter<1 cm under endoscopic resection. 2022 Vol. 28 (5): 857-861 [Abstract] ( 154 ) HTML (1 KB)  PDF (1187 KB)  ( 412 ) 862 Analysis of Risk Factors Related to Severity Evaluation of Community-Acquired Pneumonia in Children WANG Li, YU Xiumei, DENG Qingxiu, et al DOI: 10.3969/j.issn.1006-6233.2022.05.034 Objective: To analyze and study the risk factors related to the severity of community-acquired pneumonia in children, in order to provide a basis for accurately determining the severity of the disease in children with community-acquired pneumonia, to provide early diagnosis and treatment of severe community-acquired pneumonia in children, and to minimise the mortality rate. Methods: Totally 494 children diagnosed with community-acquired pneumonia and hospitalized in our hospital from October 2020 to September 2021 were divided into 428 cases of general type and 66 cases of severe type according to the diagnostic criteria of severe community-acquired pneumonia. Their general conditions, symptoms and signs, laboratory indexes and imaging characteristics were studied retrospectively, and the clinical characteristics and influencing factors of the two groups were compared and studied. Results: Compared to the 428 children with general community-acquired pneumonia, the 66 children with severe community-acquired pneumonia were younger and had a higher proportion of signs and symptoms presenting with decreased breath sounds, tachycardia, extra-pulmonary complications, pleural effusion and lobar atelectasis; in laboratory tests, C-reactive protein (CRP), lactate dehydrogenase (LDH), creatine kinase isoenzyme (CK-MB) Logistic stepwise regression analysis showed that CRP and IL-6 were independent risk factors for severe community-acquired pneumonia in children. Conclusion: Elevated serum CRP and IL-10 levels have good accuracy in determining the severity of community-acquired pneumonia in children and help in the early identification of severe community-acquired pneumonia in children. 2022 Vol. 28 (5): 862-866 [Abstract] ( 87 ) HTML (1 KB)  PDF (1203 KB)  ( 132 ) 867 Effect of Different Doses of Sildenafil on Cardiac Function and Serum CRP and MMP-9 Levels in Neonates with Persistent Pulmonary Hypertension ZHANG Weina, DING Chuangang, ZHAO Lifen, et al DOI: 10.3969/j.issn.1006-6233.2022.05.035 Objective: To investigate the effects of different doses of sildenafil on the cardiac function and serum CRP and MMP-9 levels in the treatment of neonatal persistent pulmonary hypertension. Methods: The clinical data of 125 neonates with persistent pulmonary hypertension were selected for retrospective analysis. The selection time was from February 2018 to April 2020, and they were divided according to the therapeutic dose of sildenafil into group A (0.5 mg, n= 42), group B (with 1.0 mg, n=42), group C (with 2.0 mg, n=41). The three groups were compared for C-reactive protein (CRP), matrix metalloproteinase-2 (MMP-9), N-terminal brain natriuretic peptide precursor (NT-pro BNP), right ventricular ejection fraction (RVEF), E value, A value, E/A, partial pressure of oxygen (PaO2), partial pressure of carbon dioxide (PaCO2), pulmonary artery systolic pressure (SPAP), and adverse events (nausea, vomiting, transient hypotension). Results: Repeated-measures ANOVA showed statistically significant (P<0.05) differences in time points, between-group differences and between-group interactions for CRP, NT-pro BNP, PaO2, PaCO2, SPAP, and E/A in the three groups; statistically significant (P<0.05) differences in time points and between-group interactions for MMP-9, RVEF, and E values in the three groups, while between-group differences were not statistically significant (P>0.05); the difference between time points of A values in the three groups was statistically significant (P<0.05), while the difference between groups and the interaction between time points and groups was not statistically significant (P>0.05). A two-by-two comparison showed that the differences in CRP, MMP-9, NT-pro BNP, RVEF, E, A, E/A, CRP, MMP-9 and NT-pro BNP were higher in groups B and C than in group A (P<0.05). Conclusion: Sildenafil at 1.0mg was effective in treating persistent pulmonary hypertension in neonates, improving cardiac function and reducing CRP and MMP-9 expression. 2022 Vol. 28 (5): 867-872 [Abstract] ( 147 ) HTML (1 KB)  PDF (1213 KB)  ( 128 ) 873 Clinical Efficacy of Vitamin D Combined with Montelukast in Children with Cough Variant Asthma and Its Effect on IL-17 and Treg YUE Jingwei, WANG Yan, ZHANG Min DOI: 10.3969/j.issn.1006-6233.2022.05.036 Objective: To analyze the clinical efficacy of vitamin D (VD) combined with montelukast in children with cough variant asthma (CVA) and its effect on interleukin-17 (IL-17) and regulatory T cells (Treg). Methods: Two hundred and forty-four children with CVA treated in our hospital from September 2019 to May 2021 were prospectively selected as the research objects and divided into control group (n = 61), montelukast group (n = 61), VD group (n = 61) and combined group (n = 61) through simple random method and random number table. Children in the control group were treated with routine treatment, and the other three groups were given montelukast, VD and their combination respectively for 12 weeks on the basis. The clinical efficacy and pulmonary function [including forced expiratory volume in one second (FEV1), forced vital capacity (FVC), peak expiratory flow (PEF)] and the levels of blood IL-17, CD4+CD25+Treg cells were compared among four groups. Results: The remission time and disappearance time of cough in combined group were significantly lower than those in control group, montelukast group and VD group (P<0.05). There were no significant differences in baseline data, FEV1, FVC, PEF, blood IL-17, CD4+CD25+ Treg cell levels and the incidence of adverse reactions among four groups before treatment (P>0.05). The levels of FEV1, FVC, PEF, blood CD4+CD25+Treg cells after treatment in four groups were significantly higher than those before the intervention, and the blood level of IL-17 was significantly lower than those before the intervention (P<0.05), and the application effect of the combined group was better than that of the other three groups (P<0.05). Analysis of factorial variance showed that montelukast and VD intervention were helpful to reduce cough remission time, cough disappearance time and IL-17, and increase FEV1, FVC, PEF and CD4+CD25+ Treg (P<0.05), and there was no interaction between them (P>0.05). Conclusion: VD combined with montelukast could significantly promote the remission of cough and improve lung function in children with CVA, which can be related to correcting Th17/Treg imbalance. 2022 Vol. 28 (5): 873-876 [Abstract] ( 138 ) HTML (1 KB)  PDF (1194 KB)  ( 363 )

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