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2022 Vol. 28, No. 4 Published: 2022-04-30 529 The Function and Mechanism of the Bone Marrow Mesenchymal Stem Cell Exosome MiR-25-3p in Regulating the Proliferation Migration and Invasive Ability of Osteosarcoma Cells LIU Hantao, ZHAO Lianghu, QIN Hongmin DOI: 10.3969/j.issn.1006-6233.2022.04.01 Objective: To explore the effect and mechanism of bone marrow mesenchymal stem cell exosomal miR-25-3p on the proliferation, migration and invasion of osteosarcoma cells.Methods: Bone marrow MSCs were identified by flow cytometry, bone marrow MSC exosomes (BMSC exo) were extracted and identified, miR-NC and miR-25-3p mimic were transfected into MG-63 cells (miR-NC and miR-25-3p mimic groups), 10 μg/ml BMSC exo and an equal volume of PBS were incubated with MG-63 cells for 48h (BMSC exo group and PBS group), and exosomes secreted by BMSC in the miR-NC group and miR-25-3p mimic group (miR-NC exo and miR-25-3p exo) were extracted and co-incubated with MG-63 cells for 48h (miR-NC exo group and miR-25-3p exo group). MiR -25-3p exo group MG-63 cells were transfected with PTEN plasmid (miR-25-3p exo+PTEN group). qRT-PCR was performed to detect miR-25-3p expression, CCK8 assay to detect the proliferation ability of each group, Transwell assay to detect cell migration and invasion ability, dual luciferase reporter gene to detect miR-25-3p and PTEN targeting, Western blot for PTEN expression in each cell group.Results: BMSCs expressed their marker proteins CD29 and CD90, and the number of proliferation, migration and invasion of MG-63 cells was significantly increased in the miR-25-3p mimic group compared to the miR-NC group (P<0.05). Compared with the PBS group, miR-25-3p expression was significantly increased in MG-63 cells in the BMSC exo group (P<0.001), and cell proliferation, migration and invasion numbers were significantly increased (P<0.05). PTEN was the target gene of miR-25-3p, and compared with the miR-NC exo group, miR-25-3p exo group MG-63 cells miR-25-3p expression was significantly increased (P<0.05). 25-3p expression was significantly increased (P<0.001) and cell proliferation, migration and invasion numbers were significantly increased (P<0.05) in the miR-25-3p exo group compared to the miR-25-3p exo group, and cell proliferation, migration and invasion numbers were significantly decreased (P<0.05) in the miR-25-3p exo+PTEN group.Conclusion: Bone marrow mesenchymal stem cell exosome miR-25-3p promotes proliferation, migration and invasion of osteosarcoma by targeting and inhibiting PTEN. 2022 Vol. 28 (4): 529-533 [Abstract] ( 166 ) HTML (1 KB)  PDF (2272 KB)  ( 252 ) 534 Effect and Mechanism of Action of TRIP13 on Proliferation Invasion and Angiogenesis in NSCLC DONG Yan, LI Jiawei, JIA Yinaer, et al DOI: 10.3969/j.issn.1006-6233.2022.04.02 Objective: To investigate the effects of thyroid hormone receptor interactor 13 (TRIP13) on proliferation, invasion and angiogenesis in non-small-cell lung cancer (NSCLC) and its related mechanisms. Methods: Forty-five NSCLC patients' tumours and their corresponding paraneoplastic tissues were collected. The expression of TRIP13 in the tumours and paraneoplastic tissues was measured by qRT-PCR and analysed for correlation with the patients' clinical characteristics. The expression of TRIP13 in human lung adenocarcinoma cell line A549 and lung squamous carcinoma cell line SKMES1 was measured. PLK-TRIP13 was transfected into A549 and SKMES1 by lentiviral transfection, and its relationship with NSCLC cell proliferation, invasion and angiogenesis was analysed.Results: TRIP13 was highly expressed in tumor tissues compared with adjacent tissues. TRIP13 high expression correlated with tumor type, tumor stage, and lymph node metastasis. TRIP13 high expression significantly promoted proliferation, invasion and angiogenesis of A549 cells, but not of SKMES1 cells.Conclusion: TRIP13 can promote proliferation, invasion and angiogenesis of non-small cell lung adenocarcinoma, and detection of TRIP13 and NSCLC pathological types can serve as a potential clinical treatment option for lung adenocarcinoma. 2022 Vol. 28 (4): 534-538 [Abstract] ( 148 ) HTML (1 KB)  PDF (1957 KB)  ( 268 ) 539 Effects of Propofol on Liver Function and HMGB1/TLR4 Signaling Pathway in with Carbon Dioxide Pneumoperitoneum Rats AI Ling, XU Hui, CHEN Hao DOI: 10.3969/j.issn.1006-6233.2022.04.03 Objective: To investigate the effects of propofol on liver function and high mobility group protein box 1 (HMGB1) / Toll-like receptor 4 (TLR4) pathway in with carbon dioxide pneumoperitoneum rats. Methods: The pneumoperitoneum model rats were established by setting the pneumoperitoneum pressure of 20 mmHg for 2 hours, the successful model rats were randomly divided into pneumoperitoneum group, propofol low (5mg/kg), medium (10mg/kg), high (20mg/kg) dose group and positive control group (biphenyl diester, 25mg/kg), with 10 rats in each group; and then a sham operation group (10 healthy rats) were constructed and operated by modeling without pneumoperitoneum. Two hours after operation, the sham operation group and pneumoperitoneum group were injected with normal saline intraperitoneally, propofol groups were injected intraperitoneally with propofol solution of corresponding dose, and the positive control group was given bifendate solution by gavage. Ten hours after the administration, the abdominal aortic blood and liver tissue were taken, and the liver function indexes serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), liver tissue inflammatory factors interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) levels were detected by enzyme-linked immunosorbent assay. Hematoxylin eosin staining was used to detect the morphological changes of liver tissue. The protein expression of HMGB1, TLR4, monocyte chemotactic protein-1 (MCP-1), intercellular adhesion molecule-1 (ICAM-1) and interferon-γ (IFN-γ) in liver tissue were detected by western blot.Results: Compared with those in sham operation group, the degeneration and necrosis of hepatocytes were serious in pneumoperitoneum group, the levels of serum AST, ALT, liver tissue IL-6, TNF-α, and the protein expression of HMGB1, TLR4, MCP-1, ICAM-1 and IFN-γ were higher (P<0.05). Compared with those in pneumoperitoneum group, the degeneration and necrosis of hepatocytes in the low, medium and high dose propofol groups and the positive control group were alleviated, the levels of serum AST, ALT, liver tissue IL-6, TNF-α, and the protein expression of HMGB1, TLR4, MCP-1, ICAM-1 and IFN-γ were lower (P<0.05), and the higher the dose of propofol, the more obvious the improvement effect (P<0.05); the improvement effect of propofol high dose group on liver injury was similar to that of positive control group (P>0.05).Conclusion: Propofol can inhibit the activation of HMGB1/TLR4 pathway, reduce the expression of inflammatory factors and chemokines in liver tissue, alleviate liver injury and improve liver function in rats with pneumoperitoneum. 2022 Vol. 28 (4): 539-543 [Abstract] ( 97 ) HTML (1 KB)  PDF (1835 KB)  ( 179 ) 544 Effects of Bortezomib on Apoptosis Invasion and AMPK/mTOR/4EBP1 Pathway in Human Thyroid Carcinoma Cells HE Chunyuan, LIANG Jinhuan, WANG Cui, et al DOI: 10.3969/j.issn.1006-6233.2022.04.04 Objective: To explore the effects of bortezomib on apoptosis and invasion of human thyroid carcinoma (TC) cells and its possible mechanism. Methods: Human papillary thyroid carcinoma B-CPAP cells were cultured in vitro, and the experimental concentration and time of bortezomib were screened with MTT; thyroid carcinoma B-CPAP cells at the exponential growth stage were divided into control group, AICAR group (AMPK activator 2 mmol/L), bortezomib (50nmol/L) group, bortezomib + Compound C group (bortezomib 50nmol/L + AMPK inhibitor Compound C 5μmol/L); flow cytometry to detect the apoptosis rate of B-CPAP cells in each group; Transwell test to detect cell invasion ability; Western Blot to detect apoptosis, invasion and the expression of AMPK/mTOR/4EBP1 pathway-related proteins [B-cell lymphoma/leukemia-2 gene (Bcl-2), cysteine aspartic protease-3 (Caspases-3), E-cadherin, β-catenin, adenosine monophosphate activated protein kinase (AMPK), mammalian target of rapamycin (mTOR), eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1) and its phosphorylated protein].Results: Different concentrations of bortezomib inhibited the growth of B-CPAP cells to varying degrees. Compared with the control group, the apoptosis rate of B-CPAP cells, the expression of Caspases-3, E-cadherin, β-catenin, and the ratio of p-AMPK/AMPK were significantly higher in the AICAR and bortezomib groups (P<0.05), and the entry Transwell lower layer, expression of Bcl-2, p-mTOR/mTOR, and p-4EBP1/4EBP1 ratio were significantly lower (P<0.05), and there was no statistically significant difference between the AICAR and bortezomib groups (P>0.05); the use of AMPK inhibitor Compound C elevated the ratios of p-mTOR/mTOR, p-4EBP1/4EBP1, and significantly reduced the inhibitory effect and the pro-apoptotic effect of bortezomib on the invasion of thyroid carcinoma B-CPAP cells.Conclusion: Bortezomib inhibits the proliferation and invasion of human thyroid carcinoma cells, and induce apoptosis. Its mechanism may be related to the activation of AMPK and inhibition of mTOR and 4EBP1 activation. 2022 Vol. 28 (4): 544-549 [Abstract] ( 125 ) HTML (1 KB)  PDF (1779 KB)  ( 201 ) 550 The Mechanism of "Renshen-Maidong" in the Treatment of Antineoplastic Drug-Induced Cardiotoxicity YANG Mengxia, et al DOI: 10.3969/j.issn.1006-6233.2022.04.05 Objective: To investigate the mechanism of "Renshen-Maidong" drug pair in the treatment of antineoplastic drug-induced cardiotoxicity (ADIC) by network pharmacology. Methods: TCMSP, TCMID and TCM Database@Taiwan databases were used to screen the main active ingredients of the drugs. Swiss Target database was used to search the drugs-related targets. GengCards and DisGeNet databases were used to collect the ADIC-related disease genes. Drug targets and disease genes were subjected to Venny analysis, and enrichment analysis was performed in DAVID database. The multi-level network "herbs-effective components-targets-pathways" was constructed by Cytoscape software, and the core genes were screened out.Results: There were 42 main active ingredients in "Renshen-Maidong", which contained 769 targets, and 354 ADIC genes, of which 102 were potential targets of "Renshen-Maidong" for the treatment of ADIC. Through enrichment analysis, it was found that "Renshen-Maidong" exerted cardioprotective effects through VEGF, TNF, ErbB and other signaling pathways in the treatment of ADIC. The important genes of "Renshen-Maidong" in treating ADIC are SRC, CASP3, STAT3, and MAPK1/14 and so on.Conclusion: We constructed the network "herbs-effective components-targets-pathways" through network pharmacology, and found that the mechanism of action of "Renshen-Maidong" in treating ADIC involves multiple targets and pathways, which may be related to antioxidant stress, anti inflammation, anti apoptosis, and promoting angiogenesis, etc. 2022 Vol. 28 (4): 550-553 [Abstract] ( 97 ) HTML (1 KB)  PDF (2966 KB)  ( 287 ) 554 A Preliminary Investigation on the Effect of Norcantharidin on Ocular Inflammation and Th1/Th2 Balance in Experimental Allergic Conjunctivitis and Its Mechanism ZHUANG Hairong, WU Zidong, CHEN Xuehong, et al DOI: 10.3969/j.issn.1006-6233.2022.04.06 Objective: To explore the regulating effect and mechanism of norcantharidin (NCTD) on ocular inflammation and Th1/Th2 balance in experimental allergic conjunctivitis (AC) rats. Methods: The SD rat AC model was established by the ovalbumin (OVA) method. The rats were randomly divided into control group, model group, NCTD low-dose group and NCTD high-dose group. Except for the control group, all other groups were induced with AC model, the NCTD low-dose and high-dose groups were given NCTD by gavage at 0. 05 mg·kg-1·d-1 and 0.1 mg·kg-1·d-1 respectively for 10 days; After the last sensitization of OVA, the rats were observed and scored for ocular signs, hematoxylin-eosin (HE) staining and periodate-Schiff (PAS) staining for histopathological changes in the cornea and conjunctiva, respectively, and enzyme-linked immunosorbent assay (ELISA) for the determination of cytokines IL-2, IL-4, IL-5, IL-10 and IFN-γ in the serum. Flow cytometry was performed to detect the ratio of Th1 and Th2 cells in spleen lymphocytes, real-time fluorescence PCR was performed to detect IL-4 and IFN-γ mRNA expression in conjunctival tissue, immunofluorescence staining was performed to detect VEGF expression in corneal tissue, and western blotting was performed to detect VEGF and VEGFR2 protein expression in conjunctival tissue. Results: After the rats were modeled, conjunctival hyperemia, redness, swelling, ocular fission, tearing, increased secretions and other AC pathological phenomena appeared. The inflammation score was higher than that of the control group (P<0.01), indicating that the AC animal model was successfully constructed. In the rats treated with the NCTD low-dose group and the NCTD high-dose group, the conjunctival congestion and redness were significantly relieved, tearing and secretion were reduced, the inflammation score decreased, the corneal epithelial cells were tightly arranged, neovascularization was reduced, and the numberthe of goblet cells in the conjunctiva increased, the serum levels of IL-2, IFN-γ increase, the levels of IL-4, IL-5 and IL-10 decrease, the ratio of Th1 cells and Th1/Th2 in the spleen both increased, and the ratio of Th2 cells decreaseed. The expression of IFN-γ mRNA in the conjunctival tissue was up-regulated, and the expression of IL-4 mRNA was down-regulated (all P＜0.01); at the same time, the positive expression rate of VEGF in corneal tissue decreased, and the expression of VEGF and VEGFR2 protein in conjunctival tissue were all down-regulated (all P<0.01). In addition, the effect of the high dose of NCTD was better than that of the low dose of NCTD (all P<0.01). Conclusion: Norcantharidin can alleviate the pathological process of experimental allergic conjunctivitis rats and improve Th1/Th2 cell immune imbalance by a mechanism that may be related to the inhibition of the VEGF pathway. 2022 Vol. 28 (4): 554-560 [Abstract] ( 102 ) HTML (1 KB)  PDF (2303 KB)  ( 176 ) 561 Bioinformatics-Based Prognostic Model for Ferroptosis in Hepatocellular Carcinoma YU Yangyang, HAN Chao, LI Guang DOI: 10.3969/j.issn.1006-6233.2022.04.07 Objective: To find the ferroptosis gene and independent prognostic factors, and to construct the prognostic model of ferroptosis in HCC patients. Methods: The transcriptomic data and clinical information of TCGA-LIHC were downloaded from the TCGA database, and the expression profiles of TCGA-LIHC genes were extracted using Perl language.The expression profiling data for ferroptosis-related genes were differentially analyzed using the limma package in R. Using the org.Hs.eg in the R language. Differential ferroptosis genes were id annotated using the org.Hs.eg.db package in R, and the GO and KEGG enrichment analysis of ferroptosis genes was performed using the colorspace, stringi and ggplot2 packages in R. The expression profile information of ferroptosis-related genes and the survival time and survival status of TCGA-LIHC patients were integrated using Perl language, and ferroptosis-related genes significantly associated with the survival prognosis of LIHC patients were screened by one-way cox analysis, and then the model was constructed using multi-factor cox analysis.Prognostic models of ferroptosis for hepatocellular carcinoma were grouped using the survival package in R and KM survival analysis was performed.Risk curves and survival status maps were plotted for the ferroptosis prognosis models using the pheatmap package in R. Patients’ survival time, survival status, age, sex, and TNM stage were integrated with an ferroptosis prognosis model using Perl to perform univariate and multifactor cox analyses to find factors that could be used as independent prognostic factors and ROC curves were used to assess the prognostic factors of the model.Results: The TCGA-LIHC contained 50 normal tissues and 374 hepatocellular carcinoma tissues.The differential analysis yielded 83 differential genes of TCGA-LIHC-related ferroptosis, including 70 upregulated genes and 13 downregulated genes.GO functional enrichment analysis showed that response to oxidative stress and cellular response to oxidative stress were the major ferroptosis-related biological processes; KEGG functional enrichment analysis revealed that Ferroptosis and Central carbon metabolism in cancer were the major biological pathways. Univariate cox analysis screened out 31 genes associated with ferroptosis prognosis, and multivariate cox analysis constructed a prognostic model of 10 ferroptosis-related genes (FANCD2, ZEB1, BLOC1S5-TXNDC5, HMOX1, GABARAPL1, FLT3, IDH1, G6PD, VDAC2, MYB). The coefficients in the multivariate cox regression were as follows: ferroptosis gene risk score = (-0.3774 FANCD2 expression) + (-0.3774 ZEB1 expression) + (-0.3774 BLOC1S5-TXNDC5 expression) + (-0.3774 HMOX1 expression) + (-0.3774 GABARAPL1 expression) + (-0.3774 FLT3 expression) + (-0.3774 IDH1 expression) + (-0.3774 G6PD expression) + (-0. 3774 VDAC2 expression) + (-0.3774 MYB expression).KM survival analysis showed showed a greater advantage in survival for patients with low expression of the prognostic model, P=1.992e 08. Risk curve and survival status further validated our results. Univariate and multivariate cox analyses showed P<0.001 and P=0.004 for clinical stage, and P<0.001 for riskScore, respectively. ROC curve evaluated the AUC of 0.736 for the ferroptosis model and the AUC of 0.701 for the clinical stage, which demonstrateed the reliability of the model.Conclusion: Through bioinformatics analysis, 10 ferroptosis gene models can be used to evaluate patient prognosis. 2022 Vol. 28 (4): 561-566 [Abstract] ( 378 ) HTML (1 KB)  PDF (3245 KB)  ( 479 ) 567 Effect of Sevoflurane Based on BDNF/TrkB Signaling Pathway on Dendritic Spine Development in Rat Offspring During Late Pregnancy SU Juan, et al DOI: 10.3969/j.issn.1006-6233.2022.04.08 Objective: To explore the effect of sevoflurane anesthesia on dendritic spine development in late pregnant rat offspring and its mechanism. Methods: Pregnant rats were divided into control group, sevoflurane-1h group, sevoflurane-3h group and sevoflurane-6h group, they were anesthetized with 2.3% sevoflurane respectively. P31 offspring rats were subjected to water maze test and executed with high CO2 concentration. Brain tissue was taken for electrophysiological experiments to detect long duration enhancement in CA1 region of hippocampus, Golgi staining to assess dendritic spine status and western blot to detect BDNF/TrkB protein expression.Results: Compared to the control group, the latency to evade was not altered in the water maze experiment in late pregnant rat offspring P35 pups anesthetized with sevoflurane for 1h, whereas the latency to evade increased significantly in the sevoflurane-3h and sevoflurane-6h groups, and the number of platform crossings and quadrant III dwell time decreased significantly. In addition, sevoflurane anesthesia at 3h and 6h caused a significant decrease in the slope of fEPSP and a significant decrease in the density and length of dendritic spines in the pups, however, there was no effect on the fEPSP and dendritic spine status in the sevoflurane-1h group. At the same time, the expression of PSD-95, BDNF and p-TrkB in hippocampal tissues of young rats was significantly reduced by 3h and 6h sevoflurane anesthesia.Conclusion: The reduced learning and memory capacity in late pregnant rat pups after prolonged anaesthesia is caused by reduced BDNF expression, which results from reduced p-TrkB expression mediating reduced PSD-95 protein, leading to impaired dendritic spine development. 2022 Vol. 28 (4): 567-571 [Abstract] ( 72 ) HTML (1 KB)  PDF (1671 KB)  ( 167 ) 572 Effect of Allicin Modulation of PI3K/Akt/Nrf2 Pathway on Endometriosis in Rats DONG Xiujuan, CAO Ruoyu, YU Hongji, et al DOI: 10.3969/j.issn.1006-6233.2022.04.09 Objective: To investigate the effect of Allicin on endometriosis (EMs) in rats and its mechanism. Methods: The EMs rat model was prepared by auto-internal membrane transplantation, and the 30 model rats were randomly divided into model group, Allicin (10mg/kg) group and Allicin (10mg/kg) + LY294002 (PI3K specific inhibitor, 40mg/kg) group, n=10; another 10 rats were set as the sham operation group. The drugs were given once daily by intraperitoneal injection in each group (the rats in sham operation group and model group were given normal saline) for 4 weeks. The adhesion scores of endometriosis lesions was detected, the lesion volume was measured by calipers, the endometrium pathological examination was examed by HE staining method, the content of TNF-α, IL-1β, IL-6, IFN-γ were detected by ELISA method, the content of MDA and the activity of MPO, SOD, CAT in endometriosis lesions were detected by biochemical analysis method; the expression of Akt, p-Akt, Nrf2, HO-1, NF-κB were detected by Western blot method.Results: Compared with the sham operation group, the interstitium of endometriosis lesions in the model group is dense, blood vessels are proliferated and inflammatory cell infiltration changes. The contents of TNF-α, IL-1β, IL-6, IFN-γ, MDA in the ectopic lesion tissue were increased and the activities of MPO, SOD, CAT were decreased (P<0.01); the expression of p-Akt, Nrf2, HO-1 were down-regulated and the ratio of p-Akt/Akt was decreased (P<0.01), while the expression of NF-κB was up-regulated (P<0.01). Compared with the model group, the lesion volume and adhesion score were significantly decreased in Allicin group and Allicin + LY294002 group (P<0.05 or P<0.01); ectopic lesions include epithelial cell shedding, interstitial porosity, neovascularization; the content of TNF-α, IL-1β, IL-6, IFN-γ, MDA were decreased while the activities of MPO, SOD, CAT were increased (P<0.05 or P<0.01); the expression of p-Akt, Nrf2, HO-1 were up-regulated and the ratio of p-Akt/Akt was increased (P<0.01), while the expression of NF-κB was down-regulated (P<0.01). Compared with the Allicin group, the lesion volume and adhesion score were significantly increased in Allicin + LY294002 group (P<0.01), the angiogenesis in ectopic lesions was increased; the content of TNF-α, IL-1β, IL-6, IFN-γ, MDA were increased and the activities of MPO, SOD, CAT were decreased (P<0.01); the expression of p-Akt, Nrf2, HO-1, NF-κB were down-regulated and the ratio of p-Akt/Akt was decreased (P<0.01), while the expression of NF-κB was up-regulated (P<0.01).Conclusion: Allicin has a certain therapeutic effect on EMs in rats; which mechanism may be related to the activation of PI3K/Akt/Nrf2 pathway, thereby inhibiting inflammation and oxidative stress. 2022 Vol. 28 (4): 572-577 [Abstract] ( 134 ) HTML (1 KB)  PDF (2044 KB)  ( 210 ) 578 IRF1 Regulated by MiR-23a to Inhibit Proliferation and Promote Apoptosis in Tongue Squamous Carcinoma Cells SUN Jihong, LIU Xue, et al DOI: 10.3969/j.issn.1006-6233.2022.04.010 Objective: To study the effect of IRF1 gene on proliferation and apoptosis of tongue squamous carcinoma cells and its upstream regulation mechanism by miR-23a. Methods: The expression level of IRF1 gene was detected by Real-time PCR in tongue squamous carcinoma tissues. The overexpression plasmid of IRF1 was constructed, and the effects of IRF1 on proliferation and apoptosis of tongue squamous carcinoma cells were examined by MTT assay and TUNEL assay, respectively. Bioinformatic predictions as well as fluorescent reporter vector assays verified that IRF1 was a direct target gene for miR-23a. The expression level of miR-23a in tongue squamous carcinoma tissues and the alteration of proliferation and apoptosis of tongue squamous carcinoma cells after the expression of miR-23a was further examined.Results: IRF1 expression levels were significantly lower in tongue squamous carcinoma tissues compared to paraneoplastic normal tissues. Overexpression of IRF1 in tongue squamous carcinoma cells resulted in a significant decrease in cell growth activity and an increase in paclitaxel-induced apoptosis index. TargetScan predicted that miR-23a could bind to the 3 'UTR of IRF1. It was confirmed that miR-23a could directly bind to the 3'UTR of IRF1 and thus regulate the expression of IRF1. The expression of miR-23a was abnormally elevated in tongue squamous carcinoma tissues, and after the expression of miR-23a was blocked, the growth activity of tongue squamous carcinoma cells was significantly decreased, while the apoptosis index induced by paclitaxel was increased, which was consistent with the results obtained from overexpression of IRF1. Conclusion: IRF1 is regulated by miR-23a to inhibit the growth activity and promote apoptosis of tongue squamous carcinoma cells. IRF1 is a tumor suppressor gene in the occurrence and development of tongue squamous cell carcinoma, while miR-23a, as an upstream regulator of IRF1, is also an oncogene of tongue squamous cell carcinoma. 2022 Vol. 28 (4): 578-583 [Abstract] ( 95 ) HTML (1 KB)  PDF (1915 KB)  ( 152 ) 584 Clinical Efficacy of Transjugular Intrahepatic Portosystemic Shunt Combined with Functional Splenic Embolization in the Treatment of Portal Hypertension in Cirrhotic Decompensated Liver DONG Yang, LIANG Guijie, et al DOI: 10.3969/j.issn.1006-6233.2022.04.011 Objective: To study the clinical efficacy of transjugular intrahepatic portosystemic shunt (TIPS) combined with functional splenic embolization (FSE) in the treatment of portal hypertension in decompensated cirrhosis. Methods: A total of 100 patients with cirrhotic portal hypertension were randomly divided into control group and treatment group. In the control group, partial splenic artery embolization (PSE) was performed, while in the treatment group, transjugular intrahepatic portosystemic shunt (TIPS) and functional splenic embolization (FSE) were performed. The clinical efficacy of the two groups was evaluated after one intervention. The main observations were portal vein pressure, liver function, spleen volume, blood count, coagulation, recent survival and safety indicators in both groups. Results: After treatment, the portal vein pressure, liver function, spleen volume, blood routine and coagulation function in the treatment group were better than those in the control group (P<0.05); there was no statistical difference between the two groups in terms of recent survival and safety (P>0.05). Conclusion: Transjugular intrahepatic portosystemic shunt (TIPS) combined with functional splenic embolization (FSE) has a good clinical effect in the treatment of portal hypertension in cirrhotic decompensated liver, which is worthy of clinical promotion. 2022 Vol. 28 (4): 584-588 [Abstract] ( 110 ) HTML (1 KB)  PDF (1313 KB)  ( 207 ) 589 The Expression of PECAM-1 LRRFIP PagT in Patients with Gestational Hypertension and the ROC Analysis of Predicting Preeclampsia LI Yanru DOI: 10.3969/j.issn.1006-6233.2022.04.012 Objective: To investigate the expression of platelet endothelial cell adhesion molecule 1 (PECAM-1), leucine-rich repeat interacting protein (LRRFIP), and platelet aggregation rate (PagT), and to investigate the value in predicting preeclampsia(PE), in patients with Gestational hypertension (GH). Methods: A total of 93 GH patients admitted to our hospital from April 2018 to February 2021 were selected and divided into eclampsia group (n=22), PE group (n=31), and GH group (n=40) according to the progress of the disease. And 35 healthy pregnant women were made the control group. The baseline data of each group, PECAM-1, LRRFIP, PagT, systolic blood pressure, diastolic blood pressure, and urine protein quantification were compared; Pearson was used to analyze the quantitative relationship between PECAM-1, LRRFIP, PagT and systolic blood pressure, diastolic blood pressure, and urine protein; multi-factor Logistic regression equation was used to analyze the related factors of PE and eclampsia; the receiver operating characteristic curve (ROC) and area under ROC (AUC) were used to analyze the value of PECAM-1, LRRFIP, and PagT in predicting PE and eclampsia. Results: PECAM-1: eclampsia group<PE group<GH group<control group; LRRFIP, PagT, systolic blood pressure, diastolic blood pressure, urine protein quantification: eclampsia group>PE group>GH group>control group (P<0.05); PECAM-1 is negatively correlated with systolic blood pressure, diastolic blood pressure, and urine protein quantification. LRRFIP and PagT are positively correlated with systolic blood pressure, diastolic blood pressure, and urine protein quantification (P<0.05). After the systolic blood pressure, diastolic blood pressure, and urine protein quantification are controlled, PECAM-1, LRRFIP, PagT are still related to the occurrence of PE and eclampsia (P<0.05). Predictors of AUC of PE : PECAM-1 is 0.723, LRRFIP is 0.761, PagT is 0.682, the combination of the three is 0.804; predictors of AUC of epilepsy: PECAM-1 is 0.777, LRRFIP is 0.779, PagT is 0.735, and the combination of the three is 0.850. Conclusion: The expressions of PECAM-1, LRRFIP and PagT in GH patients are related to the occurrence of PE and eclampsia. Combined detection can be used as an effective regimen to predict PE and eclampsia, and provide references for clinical monitoring and intervention. 2022 Vol. 28 (4): 589-593 [Abstract] ( 72 ) HTML (1 KB)  PDF (1319 KB)  ( 148 ) 594 The Application Value of NF-κB PAI-1 and Erythrocyte Sedimentation Rate in Diagnosis and Evaluation of Coronary Artery Injury Complicated by Kawasaki Disease WANG Zhihai DOI: 10.3969/j.issn.1006-6233.2022.04.013 Objective: To explore the application of nuclear factor kappa B (NF-κB), tissue-type plasminogen activator inhibitor-1 (PAI-1), and erythrocyte sedimentation rate (ESR) in the diagnosis and evaluation of coronary artery injury complicated by Kawasaki disease. Methods: A total of 245 children with Kawasaki disease in our hospital from May 2017 to April 2021 were selected as the research subjects. According to the results of coronary ultrasound examination, they were divided into observation group (complicated by coronary artery injury, n=42) and control group (without coronary artery injury, n=203). The clinical data, NF-κB, PAI-1, and ESR levels of the two groups were compared, the factors affecting the incidence of Kawasaki disease complicated by coronary artery injury were analyzed, the receiver operating curve (ROC) was plotted, and the level of NF-κB, PAI-1, and ESR was evaluated. And then the levels of NF-κB, PAI-1 and ESR in children with different degrees of coronary artery dilation were compared, and the relationship between the levels of NF-κB, PAI-1, ESR and the degree of coronary artery dilation was analyzed. Results: The proportion of male in the observation group, platelet count, starting time of intravenous immunoglobulin, duration of fever, NF-κB, PAI-1, and ESR levels were higher than those in the control group (P<0.05); Logistic regression analysis showed that male, platelet count, starting time of intravenous immunoglobulin, duration of fever, NF-κB, PAI-1, and ESR levels are all independent factors influencing the onset of Kawasaki disease complicated by coronary artery injury (P<0.05); Kawasaki disease complicated by coronary artery injury is a positive sample, and non-complicated coronary artery injury is a negative sample. The ROC curve of the diagnostic value of NF-κB, PAI-1, and ESR levels to Kawasaki disease complicated by coronary artery injury was plotted. The results showed that the AUCs of the diagnostic value of NF-κB, PAI- 1, ESR level for Kawasaki disease complicated by coronary artery injury were 0.814, 0.656, 0.726, respectively. Logistic binary regression fitting was used to construct the AUC of each index combined diagnosis. The results showed that the combined AUC of each index was 0.882 . With the increase of coronary artery dilation, the levels of NF-κB, PAI-1, and ESR in children increased significantly, and the difference was statistically significant (P<0.05). There is a positive correlation between the levels of NF-κB, PAI and ESR in children with Kawasaki disease complicated by coronary artery injury and the degree of coronary artery dilation (P<0.05). Conclusion: The levels of NF-κB, PAI-1 and ESR in children with Kawasaki disease complicated by coronary artery injury are significantly increased, and are positively correlated with the degree of coronary artery dilation. Clinical detection of their levels is helpful for early diagnosis and evaluation of the disease. It provides clinical basis for diagnosis and treatment. 2022 Vol. 28 (4): 594-598 [Abstract] ( 70 ) HTML (1 KB)  PDF (1498 KB)  ( 114 ) 599 SNHG15-lncRNA CeRNA Network Targets MiR-451a to Promote the Proliferation of Glioma WU Weichuan, MEN Donghai, LIU Leifeng, et al DOI: 10.3969/j.issn.1006-6233.2022.04.014 Objective: To explore the mechanism of SNHG15-lncRNA ceRNA network to promote glioma proliferation by targeting miR-451a. Methods: The expression levels of SNHG15 and miR-451a in clinical tissue samples, human glial cell lines HEB, and glioma cell lines (U87, U251 and Hs683) were detected by qRT-PCR. Dual luciferase reporter gene assay, qRT-PCR and Pull down were used to detect the targeted regulation of SNHG15-miR-451a axis. U87 cells were transfected with SNHG15 small interfering RNA (siRNA), negative control sequence (NC) and miR-451a inhibitor. The cells were randomly divided into siNC group, siSNHG15 group and siSNHG15+miR-451a inhibitor group. The regulation of invasion, migration and apoptosis of U87 cells by SNHG15 and miR-451a were detected by Transwellcell invasion, cell scratching and Tunel cell apoptosis, respectively. A subcutaneous transplantation tumor model of glioma in nude mice was constructed to detect tumor growth and cell proliferation in vivo. Results: Compared with the adjacent tissues and HEB cells, the expression of SNHG15 in glioma tissues and glioma cell lines was significantly increased, while the expression of miR-451a was significantly decreased (all P<0.05). Dual luciferase reporter gene results showed that SNHG15 luciferase activity increased significantly after transfection with miR-451a inhibitor (P<0.05). Pull down biotinylation assay showed that miR-451a decreased the expression level of SNHG15 in U87 cells (p<0.05), while the expression of miR-451a was decreased after transfection with SNHG15 plasmid (P<0.05). SNHG15 significantly inhibited, while miR-451a inhibitor significantly promoted the invasion, migration distance and apoptosis rate of U87 cells (all P<0.05). The SNHG15 group had a significantly higher graft weight than the NC group, while the miR-451a group had a significantly lower graft weight than the miR-NC group (both P<0.05) The expression of PCNA in siSNHG15 group was lower than in siNC group and siSNHG15+miR-451a inhibitor group (both P<0.05). The expression of PCNA in miR-451a inhibitor group was higher than in NC inhibitor group and siSNHG15+miR-451a inhibitor group (all P<0.05). Conclusions: SNHG15 competitively binds miR-451a to promotes the invasion and migration of glioma cell, and inhibits apoptosis. Down-regulating the SNHG15-miR-451a axis might serve as a new therapeutic target for glioma treatment. 2022 Vol. 28 (4): 599-603 [Abstract] ( 98 ) HTML (1 KB)  PDF (2846 KB)  ( 196 ) 604 Effect of Laparoscopic Hepatectomy on Immunoglobulin and VEGF and AFGF Levels in Patients with Primary Liver Cancer CHEN Jiaxin, HU Song,CHEN Yifa, et al DOI: 10.3969/j.issn.1006-6233.2022.04.015 Objective: To analyze the effects of laparoscopic hepatectomy on the levels of immunoglobulin, VEGF and aFGF in patients with primary liver cancer. Methods:A retrospective analysis was performed on 80 patients with primary liver cancer admitted to our hospital from September 2019 to September 2020, who were divided into observation group and control group according to surgical methods. In the observation group, 45 patients underwent laparoscopic hepatectomy and 35 patients in the control group underwent traditional open hepatectomy. The two groups were compared in terms of perioperative conditions, immune function indexes, vascular endothelial growth factor (VEGF) and acidic fibroblast growth factor (aFGF) levels and liver function indexes after surgery.Results: There was no statistically significant difference in the duration of surgery in the observation group compared with the control group (P>0.05); intraoperative blood loss was significantly less in the observation group compared with the control group (P<0.05); the time to first anal discharge and the total hospital stay were shorter in the observation group compared with the control group (P<0.05); immunoglobulin M (IgM), immunoglobulin A (IgA) and immunoglobulin G (IgG) levels were significantly higher in the observation group compared to the control group (P<0.05); VEGF and aFGF levels were significantly lower in the observation group compared to the control group (P<0.05); total bilirubin (TBil), alanine transaminase (ALT) and glutathione transaminase (AST) levels were significantly lower in the observation group compared to the control group after surgery (P<0.05).Conclusion: Compared with patients receiving traditional open hepatectomy, laparoscopic hepatectomy can improve perioperative conditions and reduce the inhibition of immune function. In addition, it can reduce the levels of VEGF and aFGF in patients and the injury of liver function caused by surgery. 2022 Vol. 28 (4): 604-607 [Abstract] ( 81 ) HTML (1 KB)  PDF (1196 KB)  ( 144 ) 608 Expression and Clinical Significance of MiR-4732-5p and TSPAN13 MRNA in Breast Cancer Tissues XU Fan, SHEN Yan, ZHANG Xia DOI: 10.3969/j.issn.1006-6233.2022.04.016 Objective: To investigate the expression and clinical significance of microRNA (miRNA)-4732-5p and tetraspanin 13 (TSPAN13) mRNA in breast cancer tissues. Methods: Surgical specimens of 105 breast cancer patients hospitalized in our hospital from January 2017 to August 2018 were collected. QRT-PCR was performed to detect miR-4732-5p and TSPAN13 mRNA expression in cancer and paracancerous tissues. Pearson correlation analysis was used to aalyze the correlation between miR-4732-5p and TSPAN13 mRNA expression in breast cancer tissues, and their correlation with the clinicopathologic features. The K-M method was used to plot the survival curves of breast cancer patients with different miR-4732-5p and TSPAN13 mRNA expression, Multi-factor Cox regression analysis to analyze factors influencing poor prognosis in breast cancer patients.Results: Compared with paraneoplastic tissues, miR-4732-5p expression (0.629±0.169 vs. 1.131±0.273) was elevated and TSPAN13 mRNA expression (0.529±0.081 vs. 0.355±0.078) was decreased in breast cancer tissues, with statistical differences (t=16.019, -15.790, both P<0.001). Pearson correlation analysis showed a negative correlation between miR-4732-5p and TSPAN13 mRNA expression in breast cancer tissues (r=-0.732, P<0.001). The expression of miR-4732-5p and TSPAN13 mRNA was associated with the degree of breast cancer differentiation, TNM stage, and lymph node metastasis (P<0.05 ). At 3 years follow-up, a median of 28 months, the cumulative survival rate of 105 breast cancer patients was 87.62% (92/105). The cumulative survival rate of the high miR-4732-5p expression group was lower than that of the low miR-4732-5p expression group, and the cumulative survival rate of the high TSPAN13 mRNA expression group was higher than that of the low TSPAN13 mRNA expression group (P<0.05). Multi-factorial Cox regression analysis showed that TNM stage III-IV (HR=3.381, 95% CI: 1.658 to 5.377, P=0.018), lymph node metastasis (HR=4.546, 95% CI: 1.870 to 7.758, P=0.011), miR-4732-5p≥1.131 (HR=2.932. 95% CI: 1.482 to 4.770, P=0.015) were independent risk factors for poor prognosis and TSPAN13 mRNA ≥0.355 (HR=0.310, 95% CI: 0.156 to 0.729, P=0.044) were independent protective factors for breast cancer patients.Conclusion: Upregulation of miR-4732-5p expression and downregulation of TSPAN13 mRNA expression in breast cancer tissues correlate with differentiation, TNM stage and lymph node metastasis and may be a prognostic assessment biomarker for breast cancer patients. 2022 Vol. 28 (4): 608-613 [Abstract] ( 118 ) HTML (1 KB)  PDF (1329 KB)  ( 148 ) 614 The Expression and Clinical Significance of NAMPT and Mir-206 in Serum of Patients with Chronic Heart Failure HA Sigaowa, WU Jisiguleng DOI: 10.3969/j.issn.1006-6233.2022.04.017 Objective: To investigate the expression and clinical significance of nicotinamide phosphoribose transferase (NAMPT) and mir-206 in the serum of patients with chronic heart failure. Methods: Seventy-six patients with chronic heart failure who were treated in our hospital from January 2020 to August 2021 were selected as the heart failure group. In addition, 34 healthy people who underwent physical examination in this hospital during the same period were selected as the healthy group. Enzyme-linked immunosorbent assay was used to detect the level of NAMPT in serum, real-time fluorescent quantitative PCR method to detect the level of serum mir-206; the cardiac function of patients was graded, the serum levels of NAMPT and mir-206 in patients with different cardiac function classes were compared, the correlation between NAMPT, mir-206 levels and left ventricular end diastolic diameter (LVEDD), B-type natriuretic peptide (BNP), left ventricular ejection fraction (LVEF) was analyzed, and the receiver operating characteristic curve (ROC) was used to analyze the predictive value of serum NAMPT and mir-206 levels in patients with chronic heart failure.Results: The levels of NAMPT, LVEDD and BNP in the heart failure group were higher than those in the healthy group (P<0.05), and the levels of mir-206 and LVEF were lower than those in the healthy group (P<0.05); the level of NAMPT in serum of patients with chronic heart failure gradually increased with the increase of cardiac function classification (P<0.05), and the level of mir-206 gradually decreased with the increase of cardiac function classification (P<0.05); Pearson results showed that the serum NAMPT level in patients with chronic heart failure was positively correlated with LVEDD and BNP levels (r=0.750, 0.757, P<0.05), and negatively correlated with LVEF level (r=-0.759, P<0.05); serum mir-206 level was negatively correlated with LVEDD and BNP levels (r=-0.775, -0.883, P<0.05), and positively correlated with LVEF level (r=0.819, P<0.05); ROC results showed that the area under the curve (AUC) of serum NAMPT and mir-206 levels predicting chronic heart failure was 0.853 (95% CI: 0.773-0.913) and 0.981 (95% CI: 0.935-0.998), respectively, the corresponding specificity was 61.76% and 97.06%, respectively, the sensitivity was 93.42% and 88.16%, respectively, the AUC of the combination of serum NAMPT and mir-206 predicting chronic heart failure was 0.993, with a specificity of 97.06%, and a sensitivity of 96.05%.Conclusion: The expression level of serum NAMPT in patients with chronic heart failure increases, and the expression level of mir-206 decreases. They are related to the patient's heart function classification, have certain predictive values for the occurrence of chronic heart failure and are expected to become biological indicators for the diagnosis of chronic heart failure. 2022 Vol. 28 (4): 614-618 [Abstract] ( 92 ) HTML (1 KB)  PDF (1367 KB)  ( 235 ) 619 Expression and Correlation of Serum TSH MPO and Ferritin in Hypothyroidism ZHANG Xiaocai, XU Shan, WANG Min, et al DOI: 10.3969/j.issn.1006-6233.2022.04.018 Objective: To investigate the expression and correlation of serum thyrotropin (TSH), myeloperoxidase (MPO) and ferritin in hypothyroidism. Methods: From May 2019 to May 2020, 337 patients with hypothyroidism admitted to our hospital were selected as the observation group, and 350 patients with normal thyroid function were selected as the control group during the same period. TSH and ferritin levels were measured by Roche E601 automatic electrochemiluminescence immunoassay analyzer and its supporting kit, and serum MPO expression was measured by double antibody sandwich ELISA in both groups to analyze the expression and correlation of TSH, MPO and ferritin in hypothyroid patients. Results: Compared to the control group, serum TSH and MPO were increased and ferritin, free triiodothyronine (FT3) and free thyroxine (FT4) levels were decreased in the observation group (P<0.05); correlation analysis was performed with TSH, MPO and ferritin expression. TSH and MPO were positively correlated (r=0.458/P=0.001), TSH and ferritin were negatively correlated (r=-0.368/P=0.001), MPO and ferritin were negatively correlated (r=-0.343/P=0.001); TSH and MPO were negatively correlated with changes in thyroid hormones (FT3 and FT4) in hypothyroid patients, while ferritin was positively correlated with changes in thyroid hormones in hypothyroid patients. TSH, MPO and ferritin were included in a dichotomous multi-factor logistic regression model analysis, and the results showed that TSH and MPO were risk factors and ferritin was a protective factor for the development of hypothyroidism (P<0.05). Conclusion: Serum TSH and MPO are abnormally high in hypothyroidism, and ferritin is abnormally low in hypothyroidism, which are involved in the evolution of the disease, and their detection can provide a certain reference for the diagnosis and treatment of clinical hypothyroid patients. 2022 Vol. 28 (4): 619-623 [Abstract] ( 136 ) HTML (1 KB)  PDF (1237 KB)  ( 304 ) 624 Relationship Between HMGB1 and Fas Expression Levels in Serum ofPatients with Acute Craniocerebral Injury and Postoperative Delayed Intracranial Hematoma WANG Yuyue, FAN Yunfei, YANG Xiaokun, et al DOI: 10.3969/j.issn.1006-6233.2022.04.019 Objective: To investigate the relationship betweenserum levels of high mobility group box 1 (HMGB1) and Fas and postoperative delayed traumatic intracranial hematoma (DTIH) in patients with acute craniocerebral injury. Methods: A total of 181 patients with acute craniocerebral injury admitted to the hospital between December 2018 and December 2020 were selected as the research subjects. According to postoperative CT examination results, the patients enrolled were divided into DTIH group (n=92) and non-DTIH group (n=89). Postoperative serum levels of HMGB1 and Fas, and other clinical data were compared between the two groups. The relationship between serum levels of HMGB1 and Fas and DTIH was analyzed using Logistic regression model.Results: There were statistically significant differences in serum HMGB1 and Fas between the two groups (P<0.05). The Glasgow Coma Scale (GCS) score of DTIH group was lower than that of the non-DTIH group (P<0.05), and and thrombin time (TT) was longer than that of the non-DTIH group (P<0.05). Logistic regression analysis showed that serum HMGB1, Fas, TTand GCS scorewere influencing factors of postoperative DTIH in patients with acute craniocerebral injury (P<0.05).Conclusion: The occurrence of DTIH in patients with acute craniocerebral injury may be related to serum HMGB1 and Fas. Clinical monitoring of related factors can provide an effective basis for disease diagnosis, treatment and prevention. 2022 Vol. 28 (4): 624-627 [Abstract] ( 93 ) HTML (1 KB)  PDF (1192 KB)  ( 118 ) 628 Application of VEP OCT and MfVEP in the Diagnosis and Disease Outcomes of Pediatric Optic Neuritis HUA Huilan, ZHAO Jingcong, TIAN Haixia, et al DOI: 10.3969/j.issn.1006-6233.2022.04.020 Objective: To analyze clinical characteristics of PON with different causes and to explore the value of VEP, OCT and mfVEP in the diagnosis of PON and its significance in clinical outcomes. Methods: Sixty children in the neurology ward and ophthalmology ward of Hebei Children's Hospital who were confirmed by professional physicians to be eligible for the diagnosis of optic neuritis were collected and divided into 28 cases in the isolated optic neuritis group (ON group), 11 cases in the multiple sclerosis group (MS group), 14 cases in the acute disseminated encephalomyelitis group (ADEM group) and 7 cases in the optic neuromyelitis optica spectrum disorder group (NMOSD group) according to different etiologies, and 30 children for physical examination in the same period were selected as the healthy controls. The children with PON in different groups were observed for differences in imaging, serology and eye examination. VEP, OCT and mfVEP were also performed at the first visit, at half a month, one month, three months, six months and one year to observe the changes in the disease and its outcome.Results: When comparing the MS and NMOSD groups, ON children were prone to monocular onset and ADEM children were prone to binocular onset (P<0.05); In the acute phase of the disease, vision was less affected in the ON group and in the MS group, and greatly in the ADEM and NMOSD groups (P<0.001); compared to the MS and ADEM groups, children in the ON group were more sensitive to hormones, while the NMOSD group was insensitive or less effective (P<0.05); VEP examination is meaningful for the differential diagnosis of MS, and can reflect the effect of treatment to some extent; OCT examination can visually reflectthe significant thinning of the pRNFL in the NMOSD group, which can be used as a differential diagnosis of NMOSD and can be used to assess the disease progression of NMOSD and develop the corresponding treatment regimen. The mfVEP test is better than the conventional VEP and visual field tests in detecting damage to the optic nerve and tracking recovery.Conclusion: VEP, OCT and mfVEP tests can more intuitively reflect the characteristics of children with different causes of PON, which deserves further clinical promotion. 2022 Vol. 28 (4): 628-633 [Abstract] ( 114 ) HTML (1 KB)  PDF (1219 KB)  ( 290 ) 634 Relationship Between Serum IL-17 IL-8 hs-CRP and TIMP-2 Levels and Prognosis After Tension-Free Inguinal Hernia Repair TANG Yong, ZHAO Yang, HE Bing DOI: 10.3969/j.issn.1006-6233.2022.04.021 Objective: To explore the relationship between serum interleukin 17 (IL-17), interleukin 8 (IL-8), hypersensitive C-reactive protein (hs-CRP) and tissue inhibitor of metalloproteinase (TIMP-2) levels and prognosis after tension-free inguinal hernia repair. Methods: A total of 135 inguinal hernia patients who underwent tension-free hernia repair from June 2019 to June 2020 in our hospital were selected for the study. All patients were followed up for 12 months after surgery and were divided into a good prognosis group ( 111 cases) and poor prognosis group (24 cases) based on postoperative complications and recurrence. The serum levels of IL-17, IL-8, hs-CRP and TIMP-2 were compared between the two groups, and receiver operating curve (ROC) analysis were used to determine their relationship with prognosis.Results: The levels of serum IL-17, IL-8 and hs-CRP in the poor prognosis group were higher than those in the good prognosis group, and the TIMP-2 level was lower than that in the good prognosis group, and the differences were statistically significant (P<0.05). ROC curve analysis showed that the AUCs of IL-17, IL-8, hs-CRP, and TIMP-2 in predicting poor prognosis after tension-free repair of inguinal hernia were 0.967 (0.924~1.000), 0.792 (0.690~0.895), 0.827 (0.739~0.915), 0.960 (0.925~0.996), and the Z test AUC difference showed that the AUC of IL-17 was significantly higher than that of IL-8 and hs-CRP (Z=3.099, 2.795, P＜0.05), AUC of TIMP-2 was significantly higher than that of IL-8 and hs-CRP (Z=3.053, 2.744, P<0.05). According to the optimal cutoff value, IL-17 (42.41ng/L) and IL-8 (25.43ng/L) , hs-CRP (40.14mg/L), and TIMP-2 (70.72μg/L) were 95.8%, 91.7%, 95.8%, 91.9%, and the specificities were 90.1%, 54.1%, 64.9%, 91.7%, respectively.Conclusion: Serum IL-17, IL-8, hs-CRP after tension-free repair of inguinal hernia are positively correlated with poor prognosis, and TIMP-2 levels are negatively correlated with poor prognosis, and all have a certain predictive value for poor prognosis. Among them, IL-17 , TIMP-2 has a higher predictive value. 2022 Vol. 28 (4): 634-638 [Abstract] ( 80 ) HTML (1 KB)  PDF (1345 KB)  ( 143 ) 639 Association of Serum miR-647 PTEN and In-Stent Restenosis After PCI in Patients with ACS XU Jing, BAI Jing, CUI Qianwei, et al DOI: 10.3969/j.issn.1006-6233.2022.04.022 Objective: To investigate the association of serum microRNA (miRNA)-647, phosphatase and tensin homolog deleted on chromosome ten (PTEN) with in-stent restenosis after percutaneous coronary intervention (PCI) in patients with acute coronary syndrome (ACS). Methods: A total of 209 patients with ACS were divided into restenosis group (n=45) and non-restenosis group (n=164) according to whether in-stent restenosis occurred 1 year after PCI, whose serum miR-647 and PTEN levels were measured by qPCR and ELISA. Pearson correlation was used to analyze the correlation between serum miR-647 and PTEN levels in ACS patients, multifactor Logistic regression to analyze the factors influencing in-stent restenosis after PCI in ACS patients, and ROC to analyze the predictive value of serum miR-647 and PTEN levels on in-stent restenosis after PCI in ACS patients.Results: Compared with the non-restenosis group, miR-647 levels increased and PTEN levels decreased in the restenosis group (P＜0.05). Serum miR-647 in ACS patients was negatively correlated with PTEN levels (r=-0.701, P＜0.001). Stent internal diameter, Gensini integral and PTEN were independent protective factors for in-stent restenosis after PCI, and miR-647 was an independent risk factor (P＜0.05). The AUCs for predicting in-stent restenosis after PCI were 0.768, 0.790 and 0.866 for serum miR-647 and PTEN levels at optimal cut-off values of 0.91 and 2.53 ng/mL, respectively, with sensitivities of 66.67%, 64.44% and 77.78% and specificities of 72.56%, 81.10% and 83.54%. miR-647＋PTEN predicted greater AUC than miR-647 for in-stent restenosis after PCI in ACS patients and PTEN alone (P＜0.05).Conclusion: Serum miR-647 levels are elevated and PTEN levels are decreased in ACS patients with in-stent restenosis after PCI, both of which may be jointly involved in in-stent restenosis and can be used as a predictor of in-stent restenosis. 2022 Vol. 28 (4): 639-643 [Abstract] ( 54 ) HTML (1 KB)  PDF (1474 KB)  ( 154 ) 644 Effectiveness of Parecoxib Sodium Prophylactic Analgesia Combined with Ultrasound-Fuided Transversus Abdominis Plane Block for Postoperative Analgesia After Gynecological Total Hysterectomy XIE Hong, DENG Guokui, HU Bo, et al DOI: 10.3969/j.issn.1006-6233.2022.04.023 Objective:To investigate the effect of ultrasound-guided transverse abdominal plane block (TAPB) combined with parecoxib sodium (PCX) for postoperative analgesia after gynaecological total hysterectomy and the effect on pain mediators. Methods:Eighty patients who underwent elective transabdominal total hysterectomy in our hospital from 07/2018 to 11/2021 were selected and divided into two groups of 40 patients each by simple random grouping, with the control group given TAPB compound general anesthesia and the study group given PCX on top of the control group. anesthesia was observed in both groups, and patients' levels of prostaglandin E2 (PGE2), substance P (SP), β-endorphin (β-EP), pentraxin (5-HT) and other pain mediators were measured at admission (preoperative), 6h postoperative, 12h postoperative, and 24h postoperative. (PGE2), substance P (SP), β-endorphin (β-EP), pentraxin (5-HT) and other pain mediators at the time of admission (preoperative); 6h postoperative, 12h postoperative and 24h postoperative, and pain at the time of discharge; 6h postoperative, 12h postoperative and 24h postoperative was assessed using the comfort score (BCS) and visual analogue scale (VAS).Results: In the study group, the dosage of remifentanil was significantly less than that of the control group, and the time for recovery of spontaneous breathing, awakening, first anal discharge and bed movement was significantly shorter than that of the control group (P<0.05); the levels of PGE2, SP, β-EP and 5-HT gradually increased after surgery and gradually decreased after reaching the peak at 6h postoperatively, and the levels of PGE2, SP and 5-HT in the study group were significantly lower than those in the control group at 6h, 12h and 24h postoperatively (P<0.05). The VSA score gradually increased after leaving the room, and the levels of PGE2, SP and 5-HT in the study group were significantly lower than those in the control group at 6h, 12h and 24h postoperatively (P<0.05); the BCS score gradually decreased after surgery, and the levels of PGE2, SP and 5-HT in the study group were significantly higher than those in the control group at 6h, 12h and 24h postoperatively (P<0.05); the BCS score gradually decreased after surgery, and the levels of β-EP in the study group were significantly higher than those in the control group at 6h, 12h and 24h postoperatively (P<0.05). The incidence of adverse reactions was not statistically different between the two groups (P>0.05).Conclusion: The combined application of PCX and TAPB in transabdominal total hysterectomy can effectively reduce the release of pain mediators, relieve patients' postoperative pain and improve their postoperative comfort with good safety. 2022 Vol. 28 (4): 644-648 [Abstract] ( 108 ) HTML (1 KB)  PDF (1212 KB)  ( 200 ) 649 A Controlled Study of Nasal Endoscopy-Assisted Excisional Suction Knife Surgery in Adenoid Hypertrophy ZHOU Rongjin DOI: 10.3969/j.issn.1006-6233.2022.04.024 Objective: To investigate the effectiveness of nasal endoscopy-assisted excisional suction knife surgery in the treatment of adenoid hypertrophy in patients with nasal resistance, nasal acoustic reflex, olfactory function and trauma response indicators. Methods: From February 2019 to February 2021, 106 patients with adenoid hypertrophy in our hospital were selected and divided into nasal endoscopic group (n=54) and traditional group (n=52) according to the different treatment procedures. In the nasal endoscopy group, nasal endoscopy-assisted cutting suction knife surgery was performed, and in the traditional group, blind scraping of the adenoids was performed with an adenoid scraper. Comparison was made on the efficacy, complications and trauma response indicators [tumour necrosis factor-α (TNF-α), C-reactive protein (CRP), intercellular adhesion factor-1 (ICAM-1)], nasal resistance, nasal acoustic reflexes [nasal cavity volume (NCV), nasal minimum cross-sectional area (NMCA), minimum cross-sectional distance from the anterior nostril (DCAN)], olfactory function [odour identification (OI), odour discrimination (OD), odour threshold (OT), total score (TDI)] before, at 12h and 24h after surgery, and at 6 months after surgery between the two groups.Results: After treatment, the total effective rate of 96.30% in the nasal endoscopy group was significantly higher than that of 80.77% in the traditional group (P<0.05); the serum TNF-α, CRP and ICAM-1 levels at 12h and 24h after surgery in both groups were higher than those before surgery, but lower in the nasal endoscopy group than in the traditional group (P<0.05); compared with the preoperative period, the pre-systolic and post-systolic nasal resistance and DCAN levels at 1 week after surgery in both groups were lower and NCV and NMCA levels were higher in both groups, and the nasal endoscopy group was better than the traditional group (P<0.05); compared with the preoperative period, OI, OD, OT and TDI scores were higher in both groups at 6 months postoperatively, and the scores in the nasal endoscopy group were higher than those in the traditional group (P<0.05); the postoperative complication rate was lower in the nasal endoscopy group at 6.25% compared with the traditional group at 29.03% (P<0.05). Conclusion:The use of nasal endoscopy-assisted excisional suction knife surgery to treat patients with adenoid hypertrophy can improve nasal resistance and nasal acoustic reflexes, enhance olfactory function, reduce surgical trauma, reduce complications, effectively improve the efficacy, and be safe and reliable. 2022 Vol. 28 (4): 649-654 [Abstract] ( 65 ) HTML (1 KB)  PDF (1372 KB)  ( 84 ) 655 Clinical Study of Solitaire Stent Semi-Release Protection Technique Combined with Local Thrombolysis in the Treatment of Severe Intracranial Venous Sinus Thrombosis TANG Chao, SHEN Junhui, GAO Wei, et al DOI: 10.3969/j.issn.1006-6233.2022.04.025 Objective: To explore the clinical value of Solitaire stent-retriever half-release protection (SHARP) combined with local thrombelysis in the treatment of severe intracranial sinus thrombosis (CVST). Methods: The clinical data of 143 patients with severe CVST admitted to our hospital from April 2016 to April 2020 were collected. According to the treatment protocols, 74 patients who received anticoagulation + local thrombolysis were included in the control group, and 69 patients who received anticoagulation + local thrombolysis + SHARP technique for thrombolysis were included in the study group. The duration of thrombolytic therapy, length of hospital stay, recanalization, occurrence of complications, Glasgow Coma Scale (GCS), Glasgow Outcome Scale (Glasgow Outcome Scale) and Coagulation and fibrinolysis function before and after treatment were compared between the two groups. GOS (GOS), Modified Rankin Scale (mRS) scores before and after treatment were compared between the two groups. And the prognosis of patients was evaluated according to mRS score at 6 months after treatment.Results: The duration of thrombolytic therapy in the study group was lower than that in the control group, the difference was statistically significant (P<0.05). The revascularization rate of the study group was 95.65%, higher than the control group at 77.03%, the difference was statistically significant (P<0.05). The complication rate in the study group was 4.35%, and 18.92% in the control group. The complication rate in the study group was lower than that in the control group, and the difference was statistically significant (P=0.007). The GCS score and GOS score 1 d before discharge in both groups increased and the mRS score decreased compared with those before treatment. The increase of GCS score and GOS score in the study group 1 day before discharge was higher than that in the control group, and the decrease of mRS score was higher than that in the control group, and the difference was statistically significant (P<0.05). In both groups, APTT, PT and TT were all higher and FIB and D-D were lower 1 d before discharge than before treatment; in the study group, APTT, PT and TT were all higher and FIB and D-D were all lower 1 d before discharge than in the control group. Patients in both groups were effectively followed up. At 6 months postoperatively, the good prognosis rate was 65.22% in the study group, higher than that of 32.43% in the control group, with a statistically significant difference (P=0.001).Conclusion: On the basis of anticoagulation and local thrombolysis, SHARP technique can shorten the thrombolysis time, improve the revascularization rate, reduce the incidence of complications and improve the prognosis of patients with severe CVST. 2022 Vol. 28 (4): 655-659 [Abstract] ( 87 ) HTML (1 KB)  PDF (1220 KB)  ( 245 ) 660 The Effect of Different Orthodontic Appliances on Patients' Pain Inflammatory Factors in the Gingival Sulcus and OHIP Scores JIA Yuange, LI Lei, SUO Nan DOI: 10.3969/j.issn.1006-6233.2022.04.026 Objective: To explore the influence of two correction methods of bracketless invisible appliance and self-locking bracket appliance on pain, inflammatory factors in gingival crevicular fluid and oral health score of adult orthodontic patients. Methods: A total of 108 adult orthodontic patients who were admitted from December 2018 to October 2020 were selected for the study. 54 cases were treated with self-ligating brackets (Group A) and 54 cases were treated with invisible aligners (Group B) according to the patients' wishes, and all were followed up for 6 months. The visual analogue score (VAS) was used to assess the patients' pain at 1h, 6h, 1d, 3d and 7d after treatment, to analyse the inflammatory factors in the gingival sulcus fluid before treatment and after 1, 3 and 6 months of treatment, and to assess the oral health of patients in both groups on the day of the end of the follow-up period.Results: After 6h, 1d, 3d and 7d of treatment, the VAS scores of patients in both groups showed a rising and then decreasing trend, and the scores in group B were significantly lower than those in Group A at the same time point (P<0.05).From 1 month to 6 months of treatment, the levels of IL-β and TNF-α in the gingival sulcus fluid gradually increased in both groups, and were significantly higher than those before treatment (P<0.05); the levels of IL-β and TNF-α in group B were significantly lower than those in group A at the same time point (P<0.05). After 6 months of treatment, the scores of physical pain, functional limitation, social difficulties, physical impairment, psychological communication, psychological hindrance and disability on the OHIP-14 scale were significantly higher in both groups, and the increase in each score was higher in group B than in group A (P<0.05). The composite coverage returned to normal in both groups, with 7 (12.96%) complications in group A and 4 (7.41%) in group B. The difference between the groups was not statistically significant (χ2=0.911, P=0.340). Conclusion: Compared to the self-ligating bracket method, the use of bracketless invisible aligners in adult orthodontics can reduce the patient's pain level, control the level of inflammatory factors in the sulcus and further improve the patient's oral health, which is worth promoting in relevant clinics. 2022 Vol. 28 (4): 660-664 [Abstract] ( 74 ) HTML (1 KB)  PDF (1215 KB)  ( 108 ) 665 Perioperative Infection Factors of Upper Urinary Tract Dtones and the Predictive Value of CRP/Alb DAI Chaoyun, YANG Haidong, LUO Jing, et al DOI: 10.3969/j.issn.1006-6233.2022.04.027 Objective: To explore the influencing factors of perioperative infection of upper urinary tract stones and the predictive value of C reactive protein (CRP)/serum albumin (ablumin, Alb) ratio. Methods: The clinical data of 358 patients who underwent percutaneous nephrolithotomy for stone extraction due to upper urinary tract stones admitted to our hospital from June 2018 to June 2021 were retrospectively analyzed and divided into infected group (89 cases) and uninfected group (269 cases) according to whether they had perioperative infection. The risk factors for perioperative infection were analysed using multi-factor logistic regression, comparing gender, age, Body Mass Index (BMI), site of surgery, preoperative comorbidities, history of smoking and alcohol consumption, and surgery-related indexes between the two groups. The CRP/Alb ratio was compared between the two groups, and the predictive value of the CRP/Alb ratio on the occurrence of infection was analysed using the receiver operator characteristic curve (ROC). Results: The difference between the infected group and the uninfected group in terms of smoking history, operation duration>1.5h, postoperative white blood cell count, and postoperative neutrophil ratio was statistically significant, P<0.05; The CRP/Alb ratio was significantly higher in the infected group (3.15±0.46) than in the uninfected group (1.55±0.61), P<0.05; Smoking history, postoperative white blood cell count, duration of surgery > 1.5h, and CRP/Alb ratio were all independent risk factors affecting perioperative infection of upper urinary tract stones in patients, P<0.05; ROC analysis showed that the AUC of CRP/Alb ratio for predicting perioperative infection of upper urinary tract stones was 0.979, with a sensitivity of 92.1% and specificity of 93.7%, P<0.05. Conclusion: Perioperative infection of upper urinary tract calculi is affected by various factors. The presence of smoking history, postoperative leukocyte count, duration of surgery >1.5h and high CRP/Alb ratio are all risk factors for infection and should be taken into account in clinical practice; CRP/Alb has a good predictive value for the occurrence of perioperative infection in patients with urinary tract stones, and can provide a scientific basis for clinical diagnosis and treatment. 2022 Vol. 28 (4): 665-669 [Abstract] ( 109 ) HTML (1 KB)  PDF (1321 KB)  ( 103 ) 670 Analysis of the Relationship of Mean Platelet Volume C-Reactive Protein and Fibrinogen with the Severity and Prognosis of Acute Pancreatitis DENG Zhongbo, YE Chunjuan, Xu Yulong DOI: 10.3969/j.issn.1006-6233.2022.04.028 Objective: To explore the relationship of mean platelet volume (MPV), C-reactive protein (CRP) and fibrinogen (FIB) with the severity and prognosis of acute pancreatitis (AP). Methods: This study retrospectively analyzed the data of 88 patients with AP admitted to the hospital between April 2017 and April 2021. According to the severity of the disease, the patients enrolled were divided into mild (MAP) group (n=40), moderate (MSAP) group (n=38), and severe (SAP) group (n=10). The MPV, CRP and FIB levels in the 3 groups on admission were comparatively analyzed. The correlation of MPV, CRP and FIB with the Acute Physiologic and Chronic Health Evaluation Ⅱ (APACHE Ⅱ) score, Ranson score and Bedside Index of Severity in Acute Pancreatitis (BISAP) score was analyzed. The ROC curve was plotted to evaluate the clinical prognostic value of MPV, CRP and FIB on admission, and their joint detection in AP. Results: The MPV and CRP level decreased in order from SAP group, MSAP group to MAP group (P<0.05). The MSAP group and the SAP group had higher FIB levels than the MAP group (P<0.05). However, there was no significant difference in FIB level between the MSAP group and the SAP group (P>0.05). MPV, CRP and FIB levels on admission were positively correlated with APACHE Ⅱ score, Ranson score and BISAP score (P<0.05). MPV, CRP and FIB on admission were of high prognostic value in AP (AUC=0.658, 0.668, 0.725), and their cut-off values were 12.31 fL, 84.265 mg/L, and 5.21 g/L. The area under the curve (AUC) value and sensitivity of combination of MPV, CRP and FIB3 for the prognosis of AP were significantly higher than those of MPV, CRP or FIB.Conclusion: Serum MPV, CRP and FIB are positively correlated with the severity of AP. Combination of the three can improve the prognosis of AP, which is conducive to guiding the treatment of AP. 2022 Vol. 28 (4): 670-674 [Abstract] ( 97 ) HTML (1 KB)  PDF (2594 KB)  ( 163 ) 675 Clinical Features and High-Risk Factors of Elderly Type 2 Diabetes Mellitus with Upper Gastrointestinal Hemorrhage GAO Lisha, REN Hongfei, ZHANG Mingguang DOI: 10.3969/j.issn.1006-6233.2022.04.029 Objective: To analyze the clinical features and high-risk factors of elderly type 2 diabetes mellitus (T2DM) with upper gastrointestinal hemorrhage. Methods: A total of 177 elderly patients with upper gastrointestinal hemorrhage were admitted to the hospital between May 2018 and May 2021, including 92 patients with T2DM (group A) and 85 patients without T2DM (group B). Meanwhile, 80 T2DM patients with healthy digestive tract were enrolled in this study as group C. The clinical features of group A and group B were compared. The clinical parameters of group B and group C were recorded, and the high-risk factors for elderly T2DM with upper gastrointestinal hemorrhage were analyzed.Results: There were statistically significant differences between group A and group B in terms of rhythmic abdominal pain, abdominal distension, bleeding site, and maximum diameter of ulcer (P<0.05). The response rate and Hp eradication rate in group A were lower than those in group B (P<0.05). Logistic regression analysis results showed that smoking history, drinking history, non-steroidal drug use, and Hp positive were risk factors of elderly T2DM with upper gastrointestinal hemorrhage (P<0.05).Conclusion: Elderly T2DM with UGIB have more gastric bleeding, larger gastric ulcers, and low Hp eradication rate. The risk of upper gastrointestinal hemorrhage is higher in elderly T2DM patients with smoking history, drinking history, non-steroidal drug use, and Hp-positive. 2022 Vol. 28 (4): 675-679 [Abstract] ( 86 ) HTML (1 KB)  PDF (1215 KB)  ( 186 ) 680 Therapeutic Effects of Early Surgical Clipping and Interventional Embolization in the Treatment of Ruptured Anterior Communicating Artery Aneurysms and the Influence on Perioperative Indicators and Postoperative Cmplications ZENG Weipei, WU Jie, CHEN Taidong DOI: 10.3969/j.issn.1006-6233.2022.04.030 Objective: To compare the therapeutic effects of early surgical clipping and interventional embolization in the treatment of ruptured anterior communicating artery aneurysms, and their impace on perioperative indicators and postoperative complications. Methods: Eighty-one patients with ruptured anterior communicating artery aneurysms admitted to our hospital from May 2015 to May 2020 were retrospectively selected as observation subjects, and the enrolled patients were divided into surgical clamping group (n=41) and interventional embolization group (n=40) according to the grouping of surgical methods, and the two groups were compared, and the changes in blood pressure, Glasgow coma (GOS) score, modified Rankin scale (MRS) score before and after treatment were observed. ) scores before and after treatment, and to record post-treatment outcomes and post-operative complications.Results: The duration of surgery and hospital stay were shorter in the interventional embolization group than in the surgical clipping group (P<0.05), but the cost of surgery was more than in the surgical clamping group (P<0.05); the central venous pressure, mean arterial pressure, GOS score and MRS score were significantly better in the interventional embolization group than in the surgical cliping group after treatment (P<0.05); the overall efficiency of the interventional embolization group was higher than that of the surgical cliping group [90.00% (38/40) vs. 80.49% (33/41)] (P<0.05). The difference in the incidence of adverse effects between the two groups was not statistically significant (P>0.05).Conclusion: Surgical cliping and interventional embolisation are both clinically effective and safe for ruptured anterior communicating aneurysms, with interventional embolisation being more advantageous in reducing hospital stays, restoring blood pressure and improving neurological function, but it is more expensive than surgical cliping. 2022 Vol. 28 (4): 680-683 [Abstract] ( 95 ) HTML (1 KB)  PDF (1780 KB)  ( 244 ) 684 Effect of Esketamine on Hemodynamics and Incidence of Postoperative Delirium in Patients Undergoing Coronary Artery Bypass Grafting SUN Yanbin, WANG Wenxi, AN Haiyan, et al DOI: 10.3969/j.issn.1006-6233.2022.04.031 Objective: To study the effect of esketamine on hemodynamics and postoperative delirium incidence in patients undergoing cardiac coronary artery bypass grafting.Methods: Totally 80 patients undergoing cardiac coronary artery bypass grafting (CABG) under extracorporeal circulation (CPB) in our hospital from January 2019 to June 2021 were randomly divided into a trial group and a control group, with 40 patients in each group. After admission to the operating room, the test group received 0.5mg/kg of esketamine intravenously during the induction period and 0.5mg/kg.h intravenously during the maintenance period, and the infusion was stopped at the end of the operation. In the control group, an equal amount of saline was administered. Heart rate and blood pressure were recorded at the time points of immediate admission (T0), after tracheal intubation (T1), at the time of skin incision (T2), at the time of sternotomy (T3), before CPB (T4), 10 min after CPB stop (T5), immediately after admission to ICU (T6), 6h after admission to ICU (T7), and 12h after admission to ICU (T8), respectively. The changes of left ventricular stroke volume (SV), left ventricular ejection fraction (LVEF) and cardiac output (CO) were measured by transesophageal echocardiography(TEE) at T2 and T5.The duration of mechanical ventilation, ICU stay, postoperative analgesic drug dosage and occurrence of delirium after awakening were compared.Results: The SBP of the control group at the moment of T1 was smaller than that at the moment of T0, with a statistically significant difference of P<0.05. The SBP of the experimental group at the moment of T5 was significantly higher than that of the control group; the SBP of the control group was significantly lower than that at the moment of T0 and T2; the difference was statistically significant at P<0.05. The heart rate of the experimental group was significantly higher than that of the control group at T5, with a statistically significant difference of P<0.05. The SBP of the control group at T6 was lower than that at T0, with a statistically significant difference of P<0.05. The echocardiographic cardiac function results showed that the LVEF, SV and CO levels of the experimental group were significantly better than those of the control group at T5, with a statistically significant difference of P<0.05. The incidence of delirium at 12h, 24h and 72h after removal of the tracheal tube was significantly higher than that of the control group. There was no statistically significant difference in the incidence of delirium between the two groups at 12h, 24h and 72h after extubation. The difference in the duration of mechanical ventilation and ICU stay between the two groups was statistically significant, P<0.05, with the experimental group being shorter than the control group. The amount of sufentanil used in the first 24h after surgery was less in the experimental group than in the control group, with a statistically significant difference of P<0.05.Conclusion: The use of esketamine in patients undergoing CABG with extracorporeal circulation does not increase the incidence of postoperative delirium and can make the patient more haemodynamically stable, and has the effect of reducing postoperative sufentanil dosage, shortening the duration of mechanical ventilation and ICU stay, and accelerating the patient's recovery. 2022 Vol. 28 (4): 684-689 [Abstract] ( 122 ) HTML (1 KB)  PDF (1242 KB)  ( 405 ) 690 Analysis of Hand Foot and Mouth Disease Epidemic Trends in Chengde City by ARIMA Multiplicative Seasonal Model CHI Ye, et al DOI: 10.3969/j.issn.1006-6233.2022.04.032 Objective: To establish an Autoregressive Integrated Moving Average model (Autoregressive Integrated Moving Average model, A RIMA) to predict the prevalence of HFMD in Chengde City and to provide a basis for the prevention and control of HFMD. Methods: The data were collected from the Chengde Centre for Disease Control and Prevention and the Chengde Central Hospital, and the number of HFMD cases in Chengde was counted on a monthly basis using R software, and the seasonal sparse coefficient model was used for model fitting and prediction analysis.Results: The incidence of HFMD in Chengde showed a clear seasonal distribution, with cases concentrated in the third quarter and a secondary peak in the second quarter. The ARIMA (0, 1, 2) sparse coefficient model was chosen to predict the best results for the number of incidence cases in each month in the next three years.Conclusion: The integrated moving average autoregressive model is a good fit for seasonal trends in HFMD incidence and can be used for early warning and prevention and control. 2022 Vol. 28 (4): 690-693 [Abstract] ( 81 ) HTML (1 KB)  PDF (1607 KB)  ( 264 ) 694 Clinical Study on Acupuncture and Moxibustion Combined with Huanglian Wendan Decoction in the Treatment of Stroke Sleep Disorder with Phlegm Heat Disturbing Heart Syndrome SU Guochun, ZHONG Haiyan, WEI Yin, et al DOI: 10.3969/j.issn.1006-6233.2022.04.033 Objective: To study the clinical efficacy of acupuncture and moxibustion combined with Huanglian Wendan Decoction in the treatment of stroke sleep disorder with phlegm heat disturbing heart syndrome. Methods: Totally 100 stroke patients admitted to the First People's Hospital of Qinzhou City from July 2019 to June 2020 were collected and divided into a study group and a control group, 50 cases each. Control group was treated with Huanglian Wendan Decoction, and the study group was treated with acupuncture and moxibustion combined with Huanglian Wendan Decoction, with 4 weeks as 1 course of treatment for 3 courses. The clinical efficacy of the two groups were compared, the score of TCM symptoms was evaluated, and the TCM symptom score, sleep quality by polysomnography, and neurotrophic factor levels by enzyme immunoassay were assessed before and after treatment to observe the occurrence of adverse effects.Results: The total effective rate was 94.00% in the study group, higher than that of the control group at 76.00% (P<0.05). After treatment, the TCM symptom scores (sleep disturbance, distraction and chagrin, dizziness and chest tightness, red tongue with yellow coating), as well as sleep latency, rapid eye movement sleep time, wakefulness time and Pittsburgh Sleep Quality Index score (PSQI) were lower in both groups than before treatment, and the above indicators were lower in the study group than in the control group, and the difference was statistically significant (P<0.05). After treatment, the total sleep time and serum levels of brain-derived neurotrophic factor (BDNF), glial cell-derived neurotrophic factor (GDNF) and nerve growth factor (NGF) in both groups were higher than those before treatment, and all these indexes in the study group were higher than those in the control group, and the difference was statistically significant (P<0.05). The incidence of adverse reactions during the treatment period was 14.00% and 10.00% in the study group and the control group respectively, with no difference between the groups (P>0.05). Conclusion: The combination of acupuncture and moxibustion with Huang Lian Wen Bile Tang was effective in treating patients with sleep disorders in stroke with phlegm-heat disturbing the heart, improving the patients' TCM symptoms and sleep quality, and increasing the level of neurotrophic factors in stroke patients with better treatment safety. 2022 Vol. 28 (4): 694-698 [Abstract] ( 86 ) HTML (1 KB)  PDF (1212 KB)  ( 221 )

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