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2021 Vol. 27, No. 10 Published: 2021-10-31 1585 Effects of Total Flavonoids of Ginkgo Biloba on Apoptosis of Nucleus Pulposus Cells Induced by IL-1β and Nrf2/ARE Signaling Pathway LI Jing, HUANG Yafen, et al DOI: 10.3969/j.issn.1006-6233.2021.10.001 Objective: To investigate the effect of total flavonoids of Ginkgo biloba (TFG) on apoptosis of nucleus pulposus cells induced by interleukin-1β (IL-1β), and its regulation on nuclear factor E2 related factor 2 (Nrf2)/antioxidant response element (ARE) signaling pathway. Methods: Human nucleus pulposus cells were divided into normal group, IL-1β induced group, dimethyl sulfoxide (DMSO) group and TFG groups with different concentrations (5, 10, 20, 40, 80, 160 mg/mL), in addition to the normal group, all groups were induced with IL-1β (10 ng/mL) for 24 hours, and CCK-8 method was used to detect the cell proliferation activity. The nucleus pulposus cells were divided into normal control group, IL-1β induced group, TFG low (20 mg/mL), high (80 mg/mL) dose groups, Nrf2 pathway inhibitor group (Brusatol group, 0.2 μg/mL) group, TFG + Brusatol group (80 mg/mL + 0.2 μg/mL). CCK-8 assay was used to detect cell viability; hochest 33258 staining was used to detect apoptosis; immunofluorescence was used to detect the content of reactive oxygen species (ROS); detection of Nrf2 entry by immunofluorescence cofocal method; Western blot was used to detect the expression level of Nrf2, ARE, cleaved caspase-3, tumor necrosis factor-α (TNF-α), heme oxygenase 1 (HO-1) protein. Results: Compared with that in the normal group, the proliferation activity of IL-1β induced group was lower (P<0.05). Compared with the IL-1β induced group, the proliferation activity increased with the increase of TFG concentration, it was stable at 80 mg/mL-160 mg/mL, and 20 mg/mL and 80 mg/mL were selected as TFG low and high dose groups for follow-up test. Compared with the normal control group, the cell proliferation activity of IL-1β induced group was decreased (P<0.05), and the apoptosis rate, ROS content, Nrf2 number, Nrf2, ARE, cleaved caspase-3, HO-1 and TNF-α protein expression levels were increased (P<0.05). Compared with IL-1β induced group, cell proliferation activity, Nrf2 number, Nrf2, ARE and HO-1 protein expression levels of TFG low dose and high dose groups were increased (P<0.05), cell apoptosis rate, ROS content, cleaved caspase-3 and TNF-α protein expression levels were decreased (P<0.05); and the cell proliferation activity, Nrf2 number, Nrf2, ARE and HO-1 protein expression levels in TFG high dose group were higher than those in TFG low dose group (P<0.05), and the apoptosis rate, ROS content, cleaved caspase-3 and TNF-α protein expression levels in TFG high dose group were lower than those in TFG low dose group (P<0.05); the cell proliferation activity, Nrf2 number, Nrf2, ARE and HO-1 protein expression levels of Brusatol group were decreased (P<0.05), while the apoptosis rate, ROS content, cleaved caspase-3 and TNF-α protein expression levels were increased (P<0.05). Compared with the TFG high dose group, the cell proliferation activity, Nrf2 number, Nrf2, ARE and HO-1 protein expression levels of TFG + Brusatol group were decreased (P<0.05), and the apoptosis rate, ROS content, cleaved caspase-3 and TNF-α protein expression levels were increased (P<0.05). Conclusion: TFG can play an anti-inflammatory, anti-oxidation and anti-apoptosis of nucleus pulposus cells effect by activating Nrf2/ARE pathway. 2021 Vol. 27 (10): 1585-1591 [Abstract] ( 230 ) HTML (1 KB)  PDF (2400 KB)  ( 278 ) 1592 Glycyrrhizin Relieves Liver Injury After Hematopoietic Stem Cell Transplantation by Regulating the Expression of HMGB1 FANG Ting, et al DOI: 10.3969/j.issn.1006-6233.2021.10.002 Objective: To explore the effect interaction of glycyrrhizin regulating and HMGB1 on liver injury after hematopoietic stem cell transplantation. Methods: C57BL/6 mice were selected served as the donor mices, and while BALB/C mice were the recipients. According to the initial body weight, The recipients were randomly divided into the control group, the model group and the glycyrrhizin treatment group (8 mice in each group) according to their initial body weight, with 8 in each group. Mice in the control group was were fed normallyas usual, and while mice in the model group and glycyrrhizin treatment group were operated to establish liver injury models after hematopoietic stem cell transplantationused for the establishment of hematopoietic stem cell transplantation-induced liver injury model. Mice in the glycyrrhizin treatment group were given glycyrrhizin gavage (40 mg·kg-1·d-1) for 14 days. Mice in control group and model group were given the same dose of saline. On the 15th day after transplantation, mice were sacrificed, and whole blood and liver tissues were collected. The levels of plasma HMGB1, AST, ALT, and TBIL content was were detected by ELISA, . Immunohistochemistry and RT-PCR were used to determine the level or mRNA expression of HMGB1 inthe expression of HMGB1 in liver tissue was observed by immunohistochemistry, the expression level of HMGB1 mRNA in liver tissue was detected by RT-PCRs of mice, respectively. ALT, AST and TBILcontent and inflammatory factors in liver tissue were determined by ELISA, and HE staining was used to observe the pathology of liver tissues, and explore the relationship between HMGB1 and liver injury after hematopoietic stem cell transplantation. Results: The liver function test found demonstrated that after hematopoietic stem cell transplantation, the levels of plasma ALT, AST and TBIL values of mice were significantly increased compared to the control group (P<0.01). After glycyrrhizin treatment, Glycyrrhizin treatment remarkably reduced the levels of these indexes (P<0.05). the plasma ALT level and AST level were significantly lower than that of the model group (P<0.01), TBIL was lower than the model group (P<0.05). Based on HE staining, the model group showed The results of HE staining showed that the normal structure of the liver damaged hepatic lobuless was were damaged after transplantation, the disordered arrangement of liver cells was disordered, vacuoles appeared in the cytoplasm, nuclei constricted and even necrotic, and inflammatory cell infiltration appeared. After gGlycyrrhizin gavage treatment attenuated the liver injury caused by hematopoietic stem cell transplantation, the liver tissue pathological changes were significantly improved. The results of the detection of inflammatory factors in liver tissue indicatedIn addition, we further found that: compared with the control group, the levels of TNF-α, IL-6, and IL-1β in the liver tissue of the model group were significantly increased (P<0.01). As expected, IL after treatment with glycyrrhizin reduced Tthe levels of TNF-α, and IL-6, and were significantly reduced (P<0.01), and the level of IL-1β was reduced (P<0.05). The detection of the expression level of HMGB1 showed that tThe level of HMGB1 in the plasma and liver tissues of the model group mice was significantly increased (P<0.01), and was significantly decreased after treatment with glycyrrhizin (P<0.01). The mRNA expression of HMGB1 obtained the similar patterns. the expression of HMGB1 mRNA in the liver tissue was also Show the same tren The plasma levels of ALT, AST and , TBIL, levels IL-1β, IL-6, and TNF-α of in mice in of the anti-HMGB1 group were significantly lower than those in the model group (P<0.01), while HMGB1 treatment significantly reversed the inhibitory effect of glycyrrhizin on the levels of ALT, AST and TBILthese indexes (P<0.05). The expression of IL-1β, IL-6 and TNF-α in liver tissues of mice in the anti-HMGB1 group was significantly lower than that in the model group (P<0.01), while HMGB1 treatment significantly reversed the inhibitory effect of glycyrrhizin on the expression of IL-1β, IL-6 and TNF-α ( P<0.01). Conclusion: HMGB1 has a promoting effect on liver injury after hematopoietic stem cell transplantation. Glycyrrhizin can alleviate liver injury after hematopoietic stem cell transplantation by inhibiting the expression of HMGB1, providing new ideas for the clinical treatment of liver injury after hematopoietic stem cell transplantation. 2021 Vol. 27 (10): 1592-1597 [Abstract] ( 159 ) HTML (1 KB)  PDF (1885 KB)  ( 296 ) 1598 Expression of Transcription Factors EZH2 and RUNX3 in Giant Cell Tumor of Bone and Their Relationship with Recurrence LI Tiejun, WEN Huajun, ZHENG Tiegang, et al DOI: 10.3969/j.issn.1006-6233.2021.10.003 Objective: To investigate the expression of zeste homolog 2 (EZH2) and human RUNT-related transcription factor 3 (RUNX3) in giant cell tumor of bone and its relationship with recurrence of giant cell tumor of bone. Methods: Fifty-three patients who were diagnosed with giant cell tumor of bone and were treated by surgical examination in our hospital from October 2013 to January 2018 were selected as the research subjects, and the tumor tissues and adjacent tissues (> 3 cm from the tumor tissue) of patients with giant cell tumor of bone were collected during surgery, the expressions of EZH2 and RUNX3 were detected by immunohistochemistry, and their relationships with the clinicopathological characteristics of patients were analyzed; Spearman was used for correlation analysis; and after 36 months follow-up, the expressions of EZH2 and RUNX3 were compared between the relapse group and the non-relapse group. Results: The positive expression rate of EZH2 (69.81%) in giant cell tumor of bone was higher than that in adjacent tissues (15.09%) (P<0.05), and the positive expression rate of RUNX3 (32.08%) was lower than that in adjacent tissues (86.79%) (P<0.05); the expressions of EZH2 and RUNX3 in the tumor tissues were related to tumor diameter, imaging grade, pathological grade and degree of infiltration (P<0.05); Spearman correlation analysis showed that the expressions of EZH2 and RUNX3 were negatively correlated in giant bone tumor tissue (r = -0.544, P<0.05); the positive expression rate of EZH2 in recurrent group (93.33%) was higher than that in non-recurrent group (60.53%) (P<0.05), and the positive expression rate of RUNX3 in recurrent group (0.00%) was lower than that in non-recurrent group (44.74%) (P<0.05). Conclusion: EZH2 is highly expressed in giant cell tumor of bone, and RUNX3 is lowly expressed, which is closely related to the clinicopathological characteristics of patients. The expressions of EZH2 and RUNX3 are negatively correlated, EZH2 may inhibit the expression of RUNX3, and EZH2 and RUNX3 may be used for the evaluations of the disease and prognosis of giant cell tumor of bone. 2021 Vol. 27 (10): 1598-1602 [Abstract] ( 148 ) HTML (1 KB)  PDF (1896 KB)  ( 193 ) 1603 Overexpression of NSUN2 Promoted Proliferation and Metastasis of Cervical Cancer Cells Through MiR-27b/FZD7 Axis GAO Duoduo, WANG Yao DOI: 10.3969/j.issn.1006-6233.2021.10.004 Objective: To investigate the effect of NSUN2 on proliferation, invasion and EMT of oral squamous cell carcinoma and its mechanism. Methods: The gene levels of NSUN2 in the tissues of oral squamous cell carcinoma patients, paracancerous tissues, oral squamous cell carcinoma OSCC cell lines(SCC9 and CAL27 cells)and human normal oral epithelial keratinocytes(HOK cells)were detected by qRT-PCR. The siRNA NC, NSUN2 siRNA1 and NSUN2 siRNA2 plasmids were transfected into CAL27 cells, respectively. The proliferation, invasion and EMT ability of CAL27 cells were detected by MTT, cell invasion assay and Western blot, respectively. After transfected with pcDNA-3.1(+) and pcDNA-NSUN2 plasmid, the effects of up-regulation of NSUN2 on proliferation, invasion and EMT of CAL27 cells were detected. The effect of down-regulation or overexpression of NSUN2 on the expression of NF-κB pathway related proteins was detected by Western blot. NF-κB pathway inhibitor SN50 was used to detect the effect of up-regulation of NSUN2 on the development of CAL27 cells. Results: Compared with paracancerous tissues, the expression of NSUN2 in oral squamous cell carcinoma tissues was significantly up-regulated(P<0.01).Compared with HOK cells, NSUN2 expressions in SCC9 and CAL27 cells were significantly up-regulated(P<0.01).The proliferation, invasion and EMT abilities of CAL27 cells transfected with NSUN2 siRNA1 and NSUN2 siRNA2 were significantly lower than those of the siRNA NC group, and the ratio of p-TAK1/TAK1 and NF-κB p-p65/NF-κB p65 were significantly decreased(P<0.01);The proliferation, invasion and EMT abilities of CAL27 cells in pcDNA-NSUN2 transfected group were significantly higher than those in pcDNA-3.1(+) group, and the ratios of p-TAK1/TAK1 and NF-κB p-p65/NF-κB p65 were significantly increased(P<0.01). After treated with SN50, the promoting effect of NSUN2 on the development of CAL27 cells was significantly down-regulated. Conclusion: NSUN2 was up-regulated in oral squamous cell carcinoma cells and promoted the proliferation, invasion and EMT of CAL27 cells through NF-κB pathway. 2021 Vol. 27 (10): 1603-1607 [Abstract] ( 174 ) HTML (1 KB)  PDF (1959 KB)  ( 391 ) 1608 Expression of Epithelial-Mesenchymal Transition in Gastric Carcinoma and Its Relationship with Her-2 WANG He, SHEN Zhihua, XUE Runguo, et al DOI: 10.3969/j.issn.1006-6233.2021.10.005 Objective: To observe the expression of E-cadherin and Vimentin, two epithelial-mesenchymal transition (EMT) markers and Her-2 in gastric cancer, and analyze the relationships between EMT and the clinicopathological characteristics of gastric cancer and between EMT and Her-2 expression. Methods: Immunohistochemistry was used to detect the expression of EMT markers E-cadherin and Vimentin in 198 cases of gastric cancer; at the same time, the relationship between the expression of Her-2 and EMT in gastric cancer was detected. Results: The expression of Vimentin in gastric cancer was higher than that of adjacent tissues, and the expression of E-cadherin was lower than that of adjacent tissues. The difference was statistically significant (P<0.05); The expression of E-cadherin and Vimentin had nothing to do with age and gender (P>0.05); the expression of E-cadherin and Vimentin is related to the degree of tumor differentiation, depth of invasion and lymph node metastasis (P<0.05); The expression of Vimentin in Her-2 positive gastric cancer tissues is higher than Her-2 negative tissues. The expression of E-cadherin in Her-2 positive gastric cancer tissue was lower than that in Her-2 negative tissue, and the difference was statistically significant (P<0.05). Conclusion: EMT phenomenon plays a role in the occurrence and development of gastric cancer; in gastric cancer, the expression of Her-2 influenced the expression of EMT markers. 2021 Vol. 27 (10): 1608-1611 [Abstract] ( 147 ) HTML (1 KB)  PDF (1315 KB)  ( 122 ) 1612 Correlation Analysis of HMGB1-RAGE Signaling Pathway and Pathogenesis of Preeclampsia WANG Rui, WEI Li, LU Hongtao, et al DOI: 10.3969/j.issn.1006-6233.2021.10.006 Objective: To investigate the correlation between high mobility group protein B1 (HMGB1) and its receptor advanced glycation end product (RAGE) signaling pathway and the pathogenesis of preeclampsia. Methods: 64 early epilepsy patients admitted between January 2019 and January 2021 were prospectively selected as the research objects and were divided into mild group (n = 34 cases) and severe group (n = 30) , according to different degrees of disease. 30 healthy pregnant women in the same period were selected as control group. Serum and placental tissue HMGB1, RAGE, nuclear transcription factors - kp65 (NF - kBp65) expression, HMGB1 in placental tissue, RAGE, NF - kp65 protein localization of immunohistochemical test were tested in all groups. The correlation between HMGB1, RAGE, NF- KBP65 and preeclampsia was analyzed. Results: The expressions of HMGB1, RAGE and NF-KBP65 in serum of severe group were significantly higher than those of mild group and control group, with statistical significance (P<0.05).Compared with control group and mild group, HMGB1, RAGE and NF-KBP65 in placental tissue of severe group were significantly higher, with statistical significance (P<0.05).Immunohistochemical test showed that the strong positive expressions of HMGB1, RAGE and NF-KBP65 in severe group were higher than those in mild group and control group (P<0.05), which was statistically significant. Serum HMGB1 and RAGE were positively correlated with NF-KBP65 in pregnant women with preeclampsia (r=0.387, P<0.05). Conclusion: HMGB1 and RAGE are highly expressed in serum and placental tissues of patients with preeclampsia, and are related to the severity of the disease. Considering that HMGB1-RAGE activated KBP65 signaling pathway is an important pathogenesis of preeclampsia, attention should be paid to it. 2021 Vol. 27 (10): 1612-1615 [Abstract] ( 175 ) HTML (1 KB)  PDF (1221 KB)  ( 174 ) 1616 A Study on the Distribution of Traditional Chinese Medicine Syndrome Elements in Patients with Acute Cerebral Infarction Complicated with Hypertension LIU Huanhuan, LI Benzhi, et al DOI: 10.3969/j.issn.1006-6233.2021.10.007 Objective: To study the distribution of traditional Chinese medicine (TCM) syndrome elements in patients with acute cerebral infarction complicated with hypertension. Methods: The clinical data of 195 patients with acute cerebral infarction were collected, accordance with the Diagnostic Scale of Ischemic Stroke. Then to analyze the TCM syndrome elements distribution of patients with cerebral infarction of two groups (with hypertension group and without hypertension group) by statistical methods. Results: The first three syndromes in 195 patients with acute cerebral infarction were phlegm dampness in 52 cases (26.7%), internal fire in 43 cases (22.05%) and Qi deficiency in 36 cases (18.5%).In terms of the incidence of coronary heart disease, the hypertension group (21.9%) was significantly higher than the non-hypertension group (6.1%), with statistical significance of the difference (P<0.05). In different ages, the occurrence rate of internal fire syndrome in the elderly (27.6%) was higher than that in the middle-aged(11.6%), and the occurrence rate of Qi deficiency syndrome in the middle-aged (23.2%) was higher than that in the elderly(15.4%). The above differences were statistically significant(P<0.05). There was no statistical significance in the distribution of syndrome elements in patients with acute cerebral infarction(P>0.05).The distribution of syndrome elements of hypertensive cerebral infarction patients with different levels of blood pressure was different. Compared with hypertensive cerebral infarction patients with grade III (24.0%),qi-deficiency syndrome was more common in patients with grade I(14.3%) and grade Ⅱ (5.5%), and the difference was statistically significant (P<0.05).The frequency of phlegm-dampness syndrome was higher in the non-hypertensive group (38.8%) than in the hypertensive group (22.6%), and the difference in the distribution between the two groups was statistically significant (P<0.05). Conclusion: Patients with acute cerebral infarction mainly include phlegm dampness, internal fire, and gas deficiency. Hypertension is closely related to coronary heart disease and cerebral infarction, and the distribution of TCM syndromes elements of patients with acute cerebral infarction were different at different ages and hypertension levels. Non-hypertension group had a higher proportion of sputum dampness syndrome than in hypertension group. 2021 Vol. 27 (10): 1616-1621 [Abstract] ( 119 ) HTML (1 KB)  PDF (1248 KB)  ( 153 ) 1622 Effect of Compound Anisodine Hydrobromide Injection on VEGF and IGF-1 in Patients with Non Proliferative Diabetic Retinopathy WANG Fenfen, MA Weiguo, YIN Xiaohong, et al DOI: 10.3969/j.issn.1006-6233.2021.10.008 Objective: To investigate the therapeutic effect of compound anisodine on non-proliferative diabetic retinopathy, and to provide reference for its application. Methods: According to simple random sampling and manual stratification, 146 patients with non-proliferative diabetic retinopathy admitted to the First Affiliated Hospital of Xi'an Medical College from September 2018 to September 2020 were divided into 2 groups, with 73 cases in each group. The control group was treated with total retinal laser photocoagulation (PRP)+ conventional treatment, and the observation group was treated with compound camphorine, comparison before and after treatment clinical signs (hemorrhage, edema disappeared and effusion absorption time), fundus index [vision, bleeding area, seepage area, number of micro hemangioma, within the scope of 30 o view the average sensitivity threshold (MS), retinal oscillation potential total amplitude values (oPs)], vascular endothelial growth factor (VEGF) and insulin-like growth factor number one (IGF 1) level change and clinical curative effect. Results: After treatment, the total effective rate of observation group (95.89%) was higher than that of control group (75.34%)(χ2=18.048, P=0.000). The bleeding absorption time, edema disappearance time and exudation absorption time in the observation group were significantly shorter than those in the control group, while the bleeding area and exudation area were also smaller than those in the control group (P<0.05). In terms of the difference before and after treatment, the increase in visual acuity, MS, and oPs total wave amplitude difference in the observation group was significantly greater than that in the control group (P<0.05), and the reduction of microangioma was significantly greater than that in the control group (P<0.05). The observation group had significantly greater reductions in VEGF and IGF-1 than the control group (P<0.05). Conclusion: Compound anisodine can effectively improve the clinical symptoms of patients with non-proliferative diabetic retinopathy, regulate the levels of vascular endothelial growth factor and insulin-like growth factor 1, and promote ocular microcirculation. 2021 Vol. 27 (10): 1622-1625 [Abstract] ( 122 ) HTML (1 KB)  PDF (1241 KB)  ( 243 ) 1626 In Vitro Culture and Comparative Study of Plasmacytoid and Myeloid Dendritic Cells from Mouse Bone Marrow TAN Jianfeng, LI Dongfang, GUO Quanwei, et al DOI: 10.3969/j.issn.1006-6233.2021.10.009 Objective: To establish a method for culture, isolation and identification of mouse bone marrow plasmacytoid and myeloid dendritic cell subsets in vitro, and to explore the differences of biological functions between mouse bone marrow dendritic cell subsets. Methods: Bone marrow cells of C57BL/6 mice were obtained, and the mice fms-like tyrosine kinase receptor 3 ligand (rmFlt3-L) was used to induce them to differentiate into pDC and mDC subpopulations. The DC subpopulations of mice bone marrow were separated and identified by flow cytometry and magnetic bead antibody. The cell number and the ratio of mDC to pDC at different time points were calculated to determine the best sorting time. Then, the lysates of Lewis lung cancer cells stimulated mDC and pDC, respectively, and the morphology of DC cells was observed. The changes of immunophenotype molecules in each group were detected by flow cytometry,and the secretion of cytokines was detected by ELISA, and the proliferation of lymphocytes was detected by CCK-8. Results: Mouse bone marrow cells were cultured in vitro by rmFlt3-L for 8-9 days, and the phenotypes were CD11c+CD11b-B220+pDC and CD11c+CD11b+B220-mDC. Functionally, under the condition of loading Lewis lung cancer cell lysates, the secretion of IL-12 and TNF-α in mDC group was higher than that in pDC group(P<0.05). The ability to stimulate lymphocyte proliferation in mDC group was higher than that in pDC group(P<0.05). Morphologically, mDC has large volume, irregular nucleus and many "protrusions" around the cells. However, pDC is small and round, and most cells have no "protrusion" on their surface. Immunophenotypically, the average fluorescence intensity of CD80, CD86, CD40 and MHC-Ⅱmolecules on the cell surface of mDC group loaded with Lewis lung cancer cell lysate was higher than that of pDC group. Conclusion: Mouse bone marrow cells can differentiate into pDC and mDC subpopulations under the stimulation of rmFlt3-L. The mDC loaded with tumor cell lysates has better biological function than pDC, which may be related to the morphological maturation and the up-regulation of costimulatory molecules of mDC. 2021 Vol. 27 (10): 1626-1631 [Abstract] ( 202 ) HTML (1 KB)  PDF (2503 KB)  ( 271 ) 1632 Clinical Epidemiological Characteristics and Climatic Factors of Different Subtypes of Respiratory Syncytial Virus Infection in Children WU Shouye, LIN Daojiong, WANG Yazhou DOI: 10.3969/j.issn.1006-6233.2021.10.010 Objective: To investigate the epidemiological characteristics, clinical characteristics and climatic factors of different subtypes of respiratory syncytial virus (RSV) in children. Methods: A total of 309 children with acute lower respiratory tract infection infected by RSV were collected from our hospital from January 2018 to December 2020. The epidemiological characteristics, clinical characteristics and climatic characteristics of different subtypes of RSV infection were analyzed; Spearman correlation was used to analyze the correlation between different RSV subtypes and climate characteristics. Results: Among the 309 cases of RSV, 186 cases (60.19%) of RSV-A and 123 cases (39.81%) of RSV-B were found, with RSV-A type as the dominant type; RSV-A and RSV-B groups showed statistically significant differences in different years and seasons (all P<0.05). In 2018 and 2019, RSV-B was the dominant infection group, and in 2020, RSV-A was the dominant infection group. Children infected with RSV-A and RSV-B were mainly infected under 3 years old, in winter and spring and had mild symptoms, there was no significant difference between RSV-A group and RSV-B group in terms of age and severity of disease (all P>0.05). There was no significant difference between RSV-A and RSV-B in fever, cough, wheezing, mixed infection and other clinical symptoms, course of disease and length of hospital stay (all P>0.05); Spearman correlation analysis showed that RSV-A infection was positively correlated with PM2.5, PM10, SO2, NO2 and CO (r=0.658, P<0.001; r = 0.612, P = 0.002; r = 0.501, P = 0.008; r = 0.728, P = 0.001; r =0.642, P<0.001), was negatively correlated with mean temperature, monthly total rainfall, monthly mean wind speed and O3 (r=-0.592, P=0.001; r = 0.502, P = 0.005; r = 0.618, P = 0.002; r =-0.654, P<0.001); RSV-B infection was positively correlated with CO (r=0.559, P<0.001), and negatively correlated with monthly mean temperature, monthly total rainfall and O3 (r=-0.581, P=0.001; r = 0.368, P = 0.025; r =-0.598, P<0.001). Conclusion: RSV-A type is the dominant type of RSV infection in children, and RSV-A type is the dominant type of RSV infection in children under 3 years old, winter and spring, and mild symptoms. Different subtypes of RSV infection are related to climate and environmental factors. 2021 Vol. 27 (10): 1632-1636 [Abstract] ( 129 ) HTML (1 KB)  PDF (1255 KB)  ( 173 ) 1637 Expression and the Clinical Significance of PTEN and Notch1 in Non-small cell Lung Cancer ZHANG Yuanyuan, MA Jing, HE Huina, et al DOI: 10.3969/j.issn.1006-6233.2021.10.011 Objective: To investigate the expression and clinical significance of gene of phosphate and tension homology deleted on chromosome ten (PTEN) and Notch receptor 1 (Notch1) in non-small cell lung cancer (NSCLC) tissues. Methods: 67 cases of cancer tissue specimens from NSCLC patients in our hospital from January 2016 to December 2017 were collected. At the same time, twenty-five cases of lung tissue specimens confirmed by histopathology were selected as the control group. Immunohistochemical method was used to detect the expression of PTEN and Notch1 in NSCLC tissues, and to analyze the relationship between the two and the clinical data of NSCLC patients. The patients with NSCLC were followed up for 36 months. Kendall's tau-b method was used to analyze the correlation between PTEN and Notch1 in NSCLC tissues; Kaplan-Meier survival curve was used to analyze the relationship between PTEN, Notch1 and the prognosis of NSCLC patients; COX regression was used to analyze the factors affecting the prognosis of NSCLC patients. Results: Compared with normal lung tissue, the positive expression of PTEN in NSCLC tissue was significantly reduced, and the positive expression rate of Notch1 was significantly increased, the difference was statistically significant (P<0.05). Compared with NSCLC patients without lymph node metastasis and with TNM stage I+II, NSCLC patients with lymph node metastasis and TNM stage III had a significantly lower the positive expression rate of PTEN, and a significantly higher positive expression rate of Notch1, the difference was statistically significant (P<0.05). The expression of PTEN and Notch1 in NSCLC tissue was negatively correlated (r=-0.356, P<0.05). The cumulative survival rate of patients in the PTEN positive expression group (63.0%) was higher than that in the negative expression group (44.6%); the cumulative survival rate of patients in the Notch1 positive expression group (43.1%) was lower than that in the negative expression group (63.0%). Multivariate COX analysis showed that TNM staging, positive expression of Notch1, and negative expression of PTEN were risk factors affecting the prognosis of NSCLC patients (P<0.05). Conclusion: The expression of PTEN and Notch1 in NSCLC tissues and normal lung tissues are significantly different, they are related to lymph node metastasis and TNM staging in NSCLC patients, and may be important factors in the occurrence and development of NSCLC. 2021 Vol. 27 (10): 1637-1642 [Abstract] ( 121 ) HTML (1 KB)  PDF (1680 KB)  ( 199 ) 1643 Clinical Significance the Changes of Serum Adropin and ZO-1 Levels in Patients with Hypertensive Cerebral Hemorrhage ZHANG Bin, MENG Wei, GAO Weijun, et al DOI: 10.3969/j.issn.1006-6233.2021.10.012 Objective: To explore the expression levels of Adropin and zonula occludens-1 (ZO-1) in patients with hypertensive cerebral hemorrhage and its clinical significance. Methods: A total of 143 patients with hypertensive cerebral hemorrhage in our hospital from March 2017 to March 2020 were collected and divided into mild group (n=69, NIHSS score:1~14), moderate group (n=43, NIHSS score:15~20), and severe group (n=31, NIHSS score: 21-42) according to the degree of neurological deficit. The patients also were divided into good prognosis group (n=107, GOS score: 4~5) and poor prognosis group (n=36, GOS score: 1~3) according to the prognosis 6 months after treatment. At the same time, 71 healthy volunteers were randomly selected as control group. Serum Adropin and ZO-1 levels were detected by enzyme-linked immunosorbent assay. The receiver operating characteristic (ROC) curve was plotted to evaluate the predictive value of serum Adropin and ZO-1 for poor prognosis. Results: The serum Adropin level in case group was lower than that in control group [(1.53±0.41)μg/ml vs. (3.79±0.46)μg/ml; t=36.449], and ZO-1 level was higher than that in control group [(308.22±60.93)pg/ml vs. (149.86±51.04)pg/ml],the difference was statistically significant (P<0.05). The serum Adropin in severe group and moderate group was lower than that in mild group, and ZO-1 level was higher than that in mild group. Moreover, the serum Adropin in severe group was lower than that in moderate group, and ZO-1 level was higher than that in moderate group, the difference was statistically significant (P<0.05). The Adropin level in poor prognosis group was lower than that in good prognosis group, and ZO-1 level was higher than that in good prognosis group, the difference was statistically significant (P<0.05) . Pearson product moment correlation analysis showed that serum Adropin level was negatively associated with NIHSS score (r=-0.792, P<0.05), and positively associated with GOS score (r=0761, P<0.05). Serum ZO-1 level was positively associated with NIHSS score (r=0.708, P<0.05), and negatively associated with GOS score (r=-0.673, P<0.05). The result of ROC curve showed that serum Adropin and ZO-1 had predictive value for the poor prognosis of patients with hypertensive cerebral hemorrhage 6 months after treatment, the AUC was 0.790 and 0.761 respectively, and the Adropin combined with XO-1 had higher predictive value, the AUC was 0.837, the sensitivity and specificity were 83.04% and 78.65% respectively. The predictive value of combined detection was better than that of single index (P<0.05). Conclusion: The down-regulation of serum Adropin expression and up-regualtion of ZO-1 expression are involved in the process of hypertensive cerebral hemorrhage, which are closely related to the degree of neurological deficit and prognosis. Early detection of Adropin and ZO-1 can be used as important biochemical index to judge the severity of the disease and predict the prognosis. 2021 Vol. 27 (10): 1643-1646 [Abstract] ( 164 ) HTML (1 KB)  PDF (1305 KB)  ( 389 ) 1647 The Correlation Between the Levels of Blood Uric Acid IL-2 IL-6 and TNF-α and Scores of Depressive Symptoms and Somatization Symptom in Patients with Depression MENG Yun, LI Bo DOI: 10.3969/j.issn.1006-6233.2021.10.013 Objective: To study the correlation of the levels of blood uric acid, interleukin-2(IL -2), IL-6, tumour necrosis factor-α(TNF-α) and inflammatory factors with scores of depressive symptoms and somatization symptom in patients with depression. Methods: This retrospective study included 177 patients with depression (study group) diagnosed and treated in the hospital between February 2018 and October 2020. Meanwhile, 153 healthy volunteers were selected as the control group. The levels of blood uric acid, interleukin-2 (IL-2), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the two groups were detected. The Hamilton Depression Scale (HAMD) was used to assess psychological states of the two groups. The correlation between the levels of blood uric acid and inflammatory factors and the scores of depressive symptoms and somatization symptom in patients with depression was analyzed. Results: The levels of serum IL-2, IL-6 and TNF-α in the study group were significantly higher than those in the control group, and the blood uric acid levels were significantly lower than those in the control group (P<0.05). The HAMD scores of the study group were significantly higher than those of the control group (P<0.05). The serum uric acid levels of patients with severe symptoms were significantly higher than those of patients with mild and moderate symptoms, while the levels of serum IL-2, IL-6 and TNF-α were significantly higher than those of patients with mild and moderate symptoms (P<0.05). Serum uric acid level was negatively correlated with the severity of depressive symptoms, while the levels of serum IL-2, IL-6 and TNF-α were positively correlated with the severity of depressive symptoms (P<0.05). Serum uric acid level was negatively correlated with the scores of anxiety/somatization and circadian changes (P<0.05), and there was no significant correlation with other factors (P>0.05). Serum IL-2, IL-6 and TNF-α levels were positively correlated with the scores of anxiety/somatization and circadian changes (P<0.05), but there was no significant correlation with other factors (P>0.05). Conclusion: The blood uric acid level is decreased, and inflammatory factor levels are elevated in patients with depression. The severity of depressive symptoms and somatization symptom scores are negatively correlated with blood uric acid level, and positively correlated with IL-2, IL6 and TNF-α levels. 2021 Vol. 27 (10): 1647-1651 [Abstract] ( 130 ) HTML (1 KB)  PDF (1262 KB)  ( 218 ) 1652 Changes of Serum MiR-137 and ABCA7 Levels in Patients with Alzheimer's Disease and Their Clinical Significance ZHOU Yulei, CHEN Yanjie, LIU Zemin, et al DOI: 10.3969/j.issn.1006-6233.2021.10.014 Objective: To investigate the changes and clinical significance of serum microRNA (miRNA)-137 and adenosine triphosphate binding cassette transporter 7 (ABCA7) levels in patients with Alzheimer's disease (AD). Methods: A total of 138 AD patients admitted to our hospital from January 2018 to December 2019 were selected as the AD group. According to the clinical dementia rating (CDR) scale, they were divided into mild group (n=48) and moderate group (n=59), severe group (n=31), and 64 healthy people during physical examination during the same period were selected as the control group. Spearman correlation analysis was conducted to analyze the correlation between AD group's serum miR-137, ABCA7 levels and serum Aβ42, Aβ40, Aβ42/Aβ40, blood lipid levels and MMSE score. ROC curve was plotted to analyze serum miR-137 and ABCA7 levels in the diagnosis of AD. Results: The serum miR-137, Aβ42, high-density lipoprotein cholesterol (HDL-C) levels, Aβ42/Aβ40 and MMSE scores in the AD group were significantly lower than those in the control group, and ABCA7, Aβ40, total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) levels were significantly higher than those of the control group (P<0.05). Spearman correlation analysis showed that serum miR-137 levels in AD group were positively correlated with Aβ42, Aβ42/Aβ40, HDL-C and MMSE scores, and negatively correlated with Aβ40, TC, LDL-C levels, and CDR scale (P<0.05); Serum ABCA7 levels were negatively correlated with Aβ42, Aβ42/Aβ40, HDL-C and MMSE scores, and positively correlated with Aβ40, TC, LDL-C levels, and CDR scale (P<0.05). The ROC curve showed that the area under the curve (AUC=0.925) of miR-137+ABCA7 for the diagnosis of AD was significantly greater than that of miR-137 (AUC=0.837), ABCA7 (AUC=0.823) in diagnosed alone (P<0.05), and the sensitivity and specificity were 85.51% and 84.37%. Conclusion: The level of serum miR-137 in AD patients is significantly reduced, and the level of ABCA7 is significantly increased. It is closely related to Aβ deposition, abnormal cholesterol metabolism, and disease condition in AD patients. It may be a marker for AD diagnosis and disease severity assessment. The combined detection of the two is more valuable. 2021 Vol. 27 (10): 1652-1656 [Abstract] ( 142 ) HTML (1 KB)  PDF (1324 KB)  ( 208 ) 1657 Analysis on Influence of Sequential Chemoradiation and Concurrent Chemoradiation on the Occurrence of Radiation Pneumonia in Patients with Lung Cancer and Its Relationship with Lung Function REN Lili, SONG Yiling, CHEN Yun, et al DOI: 10.3969/j.issn.1006-6233.2021.10.015 Objective: To investigate the clinical efficacy of sequential chemotherapy and concurrent chemotherapy for lung cancer, the incidence of radiation pneumonitis (RP), and the influence on lung function. Methods: A total of 60 lung cancer patients admitted to our hospital from October 2017 to October 2020 were selected. According to the random number table, they were simply randomly divided into sequential groups and synchronous groups, with 30 cases in each group. The sequential group was treated with sequential radiotherapy and chemotherapy, and the synchronous group was treated with concurrent radiochemotherapy. The clinical efficacy, incidence of radiation pneumonia and changes in lung function index levels were compared between the two groups. Results: The total effective rate of the synchronous group (70.00% vs 46.67%) was higher than that of the sequential group, but the difference was not statistically significant (P>0.05). The overall clinical efficacy of the synchronous group was significantly better than that of the sequential group, and the difference was statistically significant (P<0.05). There was no statistically significant difference in the incidence of radiation pneumonia between the two groups (P>0.05). The rank sum test showed that the overall situation of radiation pneumonia in the sequential group was more serious than that in the synchronous group, and the difference was statistically significant (P<0.05). There was no statistically significant difference in lung function indexes between the two groups before treatment (all P>0.05); after treatment, FEV1[(1.69±0.18 vs 1.42±0.31)L], FVC[(3.02±0.27 vs 2.86) in the synchronous group ±0.32)L], FEV1/FVC[(56.09±4.86 vs 49.51±8.56)%] were significantly higher than the sequential group, and the difference was statistically significant (all P<0.05). Conclusion: Compared with sequential radiotherapy and chemotherapy, the overall curative effect of concurrent radiotherapy for lung cancer is better. It can reduce the degree of radiation pneumonitis and can significantly improve the level of lung function in patients. 2021 Vol. 27 (10): 1657-1660 [Abstract] ( 96 ) HTML (1 KB)  PDF (1235 KB)  ( 190 ) 1661 Study of the Effect of Low Molecular Weiqht Heparin and Oral Anticoaqulant in Prevention Deep Venous Thrombosis and Pulmonary Embolism After Spinal Jnjury ZHENG Zilei, ZHANG Yuping, LI Haiying, et al DOI: 10.3969/j.issn.1006-6233.2021.10.016 Objective: Patients with spinal trauma are at high risk of venous thromboembolism (VTE). To compare the effects of direct oral anticoagulants (DOACs) and low molecular weight heparin (LMWH) on the outcome of patients with surgical spinal injury. Methods: A total of 760 spinal trauma surgery patients treated in our hospital from 2015 to 2020 were included. According to the treatment conditions of patients, they were divided into DOACs group and LMWH group, and the intervention rate and mortality of deep venous thrombosis (DVT), pulmonary embolism (PE), red blood cell infusion rate (PRBC) and spinal decompression surgery were compared between the two groups. Results: A total of 760 patients were included, including 415 patients who received LWMH for postoperative thrombosis prevention and 345 patients who received DOACs for postoperative thrombosis prevention. The mean age of patients was (61.73±15) years, 59% were male, and the median injury level was 12(10~18). There were no differences between the two groups in demography, vital signs, injury parameters, length of hospital stay, ivC filter placement rate and time to start thrombus prophylaxis. The incidence of hospital DVT was 5.4%, the incidence of hospital PE was 1.3%, and the mortality was 2.8%. DOACs had a lower incidence of DVT(1.2% vs 8.9%, P<0.002) and pulmonary PE (0.3% vs 2.1%, P=0.24). There were no differences in mortality, postoperative pRBC transfusion rate, or overall incidence of postoperative spinal decompression. Conclusion: DOAC can effectively reduce the incidence of DVT and PE in patients with spinal trauma surgery. This study provides a solid basis for future studies. 2021 Vol. 27 (10): 1661-1665 [Abstract] ( 111 ) HTML (1 KB)  PDF (1235 KB)  ( 165 ) 1666 Curative Effect of Percutaneous Transforaminal Endoscopic Discectomy and Interbody Fusion Decompression and Internal Fixation Under Quadrant Channel in the Treatment of Lumbar Degenerative Disease and the Influence on Perioperative Period and Complications CUI Xianfeng, DU Jiaming, DING Xin DOI: 10.3969/j.issn.1006-6233.2021.10.017 Objective: To explore the therapeutic effects of percutaneous transforaminal endoscopic discectomy (PTED) and interbody fusion decompression and internal fixation under Quadrant channel in the treatment of lumbar degenerative disease, and the influence on perioperative period and complications. Methods: 150 patients with lumbar degenerative disease treated in the hospital between January 2016 and July 2020 were selected and randomly divided into observation group (76 cases) and control group (74 cases). The control group was treated with PTED, and the observation group was treated with interbody fusion decompression and internal fixation under Quadrant channel. Related parameters were compared. Results: The operation time of the observation group was longer than that of the control group (P<0.05). The postoperative drainage volume, intraoperative blood loss, length of incision, the incidences of urinary tract infection, urinary retention, intestinal obstruction and deep vein thrombosis, and the length of hospital stay in the observation group were less, shorter or lower than those in the control group (P<0.05). The visual analogue scale (VAS) scores of the observation group were lower than those of the control group on the 1st and 3rd days after operation, and at 3 months after operation (P<0.05). There was no difference at 6 months after operation (P>0.05). 3 months after operation, the low back pain score (Oswestry Disability Index, ODI) of the observation group was lower than that of the control group (P<0.05), but there was no difference at 6 months after operation (P>0.05). 3 months after operation, the generic quality of life inventory (GQOL-74) score of the observation group was higher than that of the control group (P<0.05). Conclusion: The duration of interbody fusion decompression and internal fixation under Quadrant channel is longer, but it can significantly improve the prognosis of patients with lumbar degenerative disease, reduce pain and complications, significantly improve the patients’ quality of life and lumbar function, and promote postoperative recovery. 2021 Vol. 27 (10): 1666-1669 [Abstract] ( 70 ) HTML (1 KB)  PDF (1240 KB)  ( 167 ) 1670 Effects of Different Incisions Thoracotomy for Esophageal Cancer on Pain Lung Function and Complications in Patients with Esophageal Cancer in High Altitude Areas E Dexiu, YE Zhi, GUO Yanan, et al DOI: 10.3969/j.issn.1006-6233.2021.10.018 Objective: To explore the effects of different incisions thoracotomy for esophageal cancer on the pain, lung function and complications of patients with esophageal cancer in middle and high altitude areas. Methods: A retrospective analysis of 136 EC patients in middle and high altitude areas were admitted between August 2016 and November 2020. They were divided into 2 groups according to the surgical method. The control group of 69 cases underwent left posterior lateral thoracotomy, and the observation group of 67 cases underwent right anterolateral thoracotomy + midline upper abdominal incision treatment. The postoperative recovery of various indicators and the improvement of lung function, pain indicators, and visual analogue scale (VAS) were compared between the two groups, and the incidence rate of postoperative complications before discharge were counted. Results: There was no statistical difference in the incidence of complications between the two groups after surgery to before discharge (P>0.05). The intraoperative blood loss of the observation group was less than that of the control group (P<0.05), the intraoperative one-lung ventilation, chest cavity opening, and hospitalization length were shorter than those of the control group (P<0.05). SP, NPY, VAS scores, MVV at various postoperative periods and the improvement of VC, FEV1 and FVC levels were better than those of the control group (P<0.05). Conclusion: The treatment effect of right anterolateral thoracotomy + median incision in the upper abdomen for patients with esophageal cancer in middle and high altitude areas is significant, which can relieve pain and facilitate postoperative lung function recovery. 2021 Vol. 27 (10): 1670-1674 [Abstract] ( 134 ) HTML (1 KB)  PDF (1307 KB)  ( 188 ) 1675 Diagnostic Value of Peritoneal Dialysisdrainage Fluid IL-6 and NGAL Inperitoneal Dialysis-Associated Peritonitis ZHUANG Yijun, CHEN Wen, YUN Manli, et al DOI: 10.3969/j.issn.1006-6233.2021.10.019 Objective: To investigate the diagnostic value of peritoneal dialysis (PD) drainage fluid interleukin-6 (IL-6) and neutrophil gelatinase-associated lipocalin (NGAL) in peritoneal dialysis-associated peritonitis (PDAP). Methods: A total of 120 patients hospitalized in the nephrology department and undergoing PD in the hospital between January 2019 and September 2020 were selected as study objects. According to the presence or absence of PDAP, the patients were divided into PDAP group and non-PDAP group. The levels of drainage fluid IL-6 and NGAL in the two groups were compared, and their diagnostic valuein PDAP was analyzed. Results: Follow-up revealed that 55 of the 120 patients developed PDAP. The drainage fluid IL-6, NGAL and WBC, and dialysis age of the PDAP group were significantly higher or longer than those of the non-PDAP group (P<0.05). Drainage fluid IL-6, NGAL and WBC, and dialysis age were independent risk factors for PDAP. Both IL-6 and NGAL single detection were of high value in the diagnosis of PDAP. Conclusion: IL-6 and NGAL in drainage fluid are independent risk factors for PDAP, which provides an effective basis for the diagnosis of PDAP. 2021 Vol. 27 (10): 1675-1678 [Abstract] ( 121 ) HTML (1 KB)  PDF (1414 KB)  ( 209 ) 1679 Diagnosis of Coronary Myocardial Bridge by 256-Slice Spiral CT and DSA a Comparative Study WANG Chao, ZHUANG Xiaotun, WANG Wei, et al DOI: 10.3969/j.issn.1006-6233.2021.10.020 Objective: To compare the effects of 256-slice spiral CT angiography (CTA) and digital subtraction angiography (DSA) in the diagnosis of coronary myocardial bridge. Methods: A total of 120 patients diagnosed with coronary heart disease in the hospital from June 2017 to June 2020 were selected as the study objects. All patients were diagnosed by 256-slice CTA and DSA. The situation of coronary artery stenosis and detection rate of myocardial bridge were compared between the two groups. Results: Through 256-slice CTA, a total of 93 cases with coronary artery stenosis were verified, including 56 cases with mild stenosis, 28 cases with moderate stenosis, and 9 cases with severe stenosis. Through DSA 85 cases of coronary artery stenosis were verified, and the mild, moderate and severe stenosis were 55 cases, 21 cases and 9 cases respectively. The two methods had good consistency in the diagnosis of coronary artery disease (kappa = 0.741, P<0.001). The detection rate of coronary myocardial bridge by 256-slice CTA was 80.00%, significantly higher than 68.33% by DSA (P<0.05). Conclusion: 256-slice CTA can directly display the morphological characteristics of myocardial bridge and blood vessels. Compared with DSA, it has more advantages in the diagnosis of abnormal anatomical structure and myocardial bridge. However, this method requires high for patients’ heart rate, and CTA and DSA have high consistency in the detection of coronary artery lesions. Therefore, clinicians can flexibly apply it according to the actual situation in the actual detection process, so as to play their respective advantages and improve the detection rate of myocardial bridge in patients 2021 Vol. 27 (10): 1679-1681 [Abstract] ( 142 ) HTML (1 KB)  PDF (1605 KB)  ( 201 ) 1682 Analysis of the Diagnostic Value of DTS DES DR in Active Pulmonary Tuberculosis Parenchymal Lesions WANG Lei, et al DOI: 10.3969/j.issn.1006-6233.2021.10.021 Objective: To explore the diagnostic value of X-ray digital body fusion (DTS), digital photography double energy subtraction (DES) and direct digital X-ray imaging (DR) in active pulmonary tuberculosis parenchymal lesions. Methods: Prospective studies were conduct on 77 patients with active pulmonary parenchyma lesions between October 2019 to October 2020 with X-ray digital layer fusion technology, digital photography, digital radiography dual energy subtraction angiography tuberculosis to detect the pathological classification, signs of tuberculosis detection, and an analysis of the relationship between the indexes and active pulmonary parenchyma lesions was conducted. Results: Compared with DR and DES, the accuracy of PATHOLOGICAL classification of NODULES or masses, tracheobronchial tuberculosis, ground glass density, pneumonia consolidation and chestnut venereal disease were higher in DTS (P<0.05). Compared with DR and DES, the diagnosis of DTS tuberculosis was higher in pleural effusion, calcification foci, tuberculous cavity, and hilar mediastinal lymph node enlargement (P<0.05). Compared with DR and DES, DTS had higher diagnostic value in active pulmonary tuberculosis parenchymal lesions (P<0.05). Conclusion: DTS is effective in the differential diagnosis of active pulmonary tuberculosis parenchymal lesions, and can also effectively evaluate the pathological classification of pulmonary tuberculosis and distinguish the detection of pulmonary tuberculosis signs, which can provide an important reference for the determination of the clinical treatment of patients. 2021 Vol. 27 (10): 1682-1686 [Abstract] ( 117 ) HTML (1 KB)  PDF (3057 KB)  ( 242 ) 1687 An Analysis of the Correlation Between Serum Hcy NT-ProBNP Levels and Arrhythmia in Patients with Chronic Heart Failure MENG Cheng, LV Lei, et al DOI: 10.3969/j.issn.1006-6233.2021.10.022 Objective: To analyze the correlation between serum homocysteine (Hcy), N-terminal pro-B-type natriuretic peptide (NT-proBNP) levels and arrhythmia in patients with chronic heart failure (CHF), aiming to further investigate the pathological mechanism of arrhythmia in patients with CHF, and to provide a basis for early clinical detection and development of treatment plans. Methods: The medical data of 76 CHF patients were retrospectively analyzed. According to whether there was arrhythmia or not, 45 patients without arrhythmia were included in the non-arrhythmia group, and 31 patients with arrhythmia were included in the arrhythmia group. Another 30 healthy people who were admitted to the hospital for physical examination during the same period were selected as the control group. The general data, blood lipid, serum creatinine (Cr), serum Hcy and NT-proBNP levels of the subjects were carefully recorded. The Pearson method was used to test the correlation between serum Hcy and NT-proBNP. The general linear regression analysis was used to obtain the linear regression equation to test the effect of serum Hcy and NT-proBNP levels on the occurrence of arrhythmia in patients with CHF. The area under the curve was obtained by plotting the ROC curve, and the value of serum Hcy and NT-proBNP levels in the prediction of arrhythmia in patients with CHF was tested. Results: The levels of serum Hcy and NT-proBNP in the arrhythmia group were higher than those in the non-arrhythmia group and the control group, with a statistical significant difference (P<0.05); the levels of serum Hcy and NT-proBNP in the non-arrhythmia group were higher than those in the control group, with a statistical significant difference (P<0.05); the bivariate Pearson correlation test showed that serum Hcy was positively correlated with NT-proBNP in CHF patients with arrhythmia (r=0.371, P<0.001); the linear regression equation was obtained by linear regression analysis: Y (Hcy)=0.717+0.063X, Y (NT-proBNP)=1.016+0.002X, and serum Hcy and NT-proBNP may be the influencing factors for inducing arrhythmia in patients with CHF (P<0.05); the arrhythmia in CHF patients was used as a state variable, and the ROC curve was plotted, and the results showed that the areas under the curve of serum Hcy and NT-proBN levels in predicting the occurrence of arrhythmia in CHF patients were 0.857 (95%CI: 0.775-0.939) and 0.913 (95%CI: 0.860-0.965), the sensitivity was 86.71% and 90.00%, and the specificity was 68.45%, 71.10%, respectively. Conclusion: Serum Hcy and NT-proBNP levels are closely related to arrhythmia in patients with CHF, which may be the influencing factors for arrhythmia in patients with CHF. Clinical evaluation of serum Hcy and NT-proBNP levels in patients with CHF has higher application value in predicting the occurrence of arrhythmia. 2021 Vol. 27 (10): 1687-1691 [Abstract] ( 195 ) HTML (1 KB)  PDF (1561 KB)  ( 134 ) 1692 The Evaluation Value of Serum Activin-A Combined with Lung Ultrasound Score for the Severity and Prognosis of Acute Respiratory Dstress Syndrome SHU Jiaojie, ZHANG Yan, GUO Yumei, et al DOI: 10.3969/j.issn.1006-6233.2021.10.023 Objective: To explore the evaluation value of serum Activin-A (Activin-A) combined with lung ultrasound score (LUS) for the severity and prognosis of acute respiratory distress syndrome (ARDS). Methods: 160 ARDS patients admitted to the intensive care unit (ICU) of our hospital (January 2018 to December 2020) were selected. According to the severity of the disease, they were divided into severe group (n=80), moderate group (n=52), mild group (n=28); according to the 28d prognosis, they were divided into death group (n=40) and survival group (n=120), the other 60 healthy people were selected as the control group during the same period.. Detection of serum Activin-A and LUS, Pearson correlation analysis of serum Activin-A level, LUS and oxygenation index (OI) correlation, multivariate Logistics regression analysis of adverse prognostic factors of ARDS, ROC curve to determine the evaluation value of serum Activin-A combined with LUS on the poor prognosis of ARDS patients. Results: Serum Activin-A levels and LUS were significantly higher in the ARDS group than in the control group (P＜0.05). Serum Activin-A levels and LUS were significantly higher in the severe group than in the mild and moderate groups, and serum Activin-A levels and LUS were significantly higher in the moderate group than in the mild group (P＜0.05).Pearson correlation analysis showed that serum Activin-A levels and LUS were negatively correlated with OI (P<0.05). Multivariate logistic regression analysis showed that OI (OR=0.957, 95%CI: 0.932~0.981) was an independent protective factor for poor prognosis of ARDS, Activin-A (OR=1.012, 95%CI: 1.006~1.018), LUS (OR=1.328, 95%CI: 1.128~1.564) were independent risk factors (P<0.05). The ROC curve showed that the area under the curve (AUC) of Activin-A+LUS (AUC=0.922, 95%CI: 0.869~0.958) predicting poor prognosis of ARDS patients was significantly greater than that of Activin-A (AUC=0.841, 95%CI: 0.775~0.894), LUS (AUC=0.792, 95%CI: 0.721~0.852) assessment. Sensitivity, specificity, accuracy are 87.04%, 89.62%, 88.98%. Conclusion: Serum Activin-A and LUS are closely related to the severity and prognosis of ARDS, and combined detection can improve the prognostic value of ARDS. 2021 Vol. 27 (10): 1692-1696 [Abstract] ( 100 ) HTML (1 KB)  PDF (1472 KB)  ( 152 ) 1697 An Observation of Anesthesia Effect of Dexmedetomidine Combined with Endotracheal Laryngeal Mask Intubation in Cervical Surgery ZHANG Xuewei, SUN Yanbin, WANG Wenxi, et al DOI: 10.3969/j.issn.1006-6233.2021.10.024 Objective: To investigate the effects of anesthesia and stress response of dexmedetomidine combined with endotracheal intubation laryngeal mask (ILMA) in patients undergoing cervical surgery. Methods: 120 patients with cervical spine surgery of ASA Ⅰ/Ⅱ from February 2017 to February 2019 in our hospital for treatment were divided into observation group and control group by simple random number table method, 60 cases in each group. The observation group was treated with dexmedetomidine combined with ILMA, while the control group was treated with normal saline combined with ILMA. Changes of hemodynamic indexes and inflammatory stress indexes [serum cortisol (COR), epinephrine (E), norepinephrine (NE), interleukin-6 (IL-6), tumor necrosis factor (TNF-α) and C-reactive protein (CRP)] of two groups of patients before anesthesia (T0), immediate intubation (T1), before extubation (T2) and 5 min after extubation (T3) were compared. Results: Compared with the control group, HR and MAP in the observation group at T1, T2 and T3 were significantly lower than those in the control group, the difference was statistically significant (P<0.05), while there was no statistical significance between the two groups at T0 (P>0.05). Compared with T0, HR and MAP in the control group were significantly increased at T1, T2 and T3, with statistical significance (P<0.05). Compared with the control group, the expression levels of stress in observation group at T1, T2 and T3 were significantly decreased, with statistical significance (P<0.05). Compared with T0, the expression levels of stress indicators in 2 groups were significantly up-regulated at T1, T2 and T3, with statistical significance (P<0.05), while there was no statistical significance between the two groups at T0 (P>0.05). Conclusion: The application of dexmedetomidine combined with endotracheal intubation laryngeal mask in cervical spine surgery can significantly reduce the inflammatory stress response and effectively maintain hemodynamic stability. 2021 Vol. 27 (10): 1697-1700 [Abstract] ( 98 ) HTML (1 KB)  PDF (1239 KB)  ( 133 ) 1701 Cosmetic Outcome of V Incision Versus S Incision in Patients with Benign Tumors of the Parotid Gland MIAO Haiping, MA Yuxia, WANG Fangfang, et al DOI: 10.3969/j.issn.1006-6233.2021.10.025 Objective: To explore the cosmetic outcomes of V incision versus S incision in patients with benign tumors of the parotid gland. Methods: A total of 189 patients with benign tumors of the parotid gland treated in our hospital from September 2018 to March 2021 were selected and divided into three groups according to the random number method: Group s (94 patients) and group V (95 patients). Group s had S incision surgery, group V had V incision surgery. Surgery related indicators [operation time, hospitalization days, blood loss, incision length, drainage volume] were compared between the two groups. Oxidative stress-related factors [norepinephrine (NE), peroxyhydrogenase (Cor), total antioxidant capacity (TAC)] were measured using enzyme-linked immunosorbent assay, and the incidence of postoperative complications as well as postoperative satisfaction rate were compared between the two groups. Results: Compared with group S, group V had less blood loss, drainage volume, and shorter incision length (P<0.05); the levels of NE, Cor, and TAC increased in both groups after surgery. Compared with those in group S, patients in group V had lower levels of NE, Cor, and TAC (P<0.05); the incidence of auricular greater nerve injury was the same in groups S and V. Group V had a lower incidence of Frey's syndrome and concave deformity of the surgical area compared with group S, and the overall incidence of complications was significantly different between the two groups (P<0.05); compared with group S, group V patients had a higher rate of satisfaction with treatment (P<0.05). Conclusion: V incision surgery for the treatment of patients with benign tumors of the parotid gland reduces the volume of intraoperative blood loss, shortens the length of the incision, reduces the occurrence of complications, increases the patient satisfaction rate, and has a better cosmetic outcome. 2021 Vol. 27 (10): 1701-1704 [Abstract] ( 115 ) HTML (1 KB)  PDF (1242 KB)  ( 162 ) 1705 Comparison of Effects of High-Flow Nasal Cannula and Non-Invasive Positive Pressure Ventilation on Oxygenation Index and Respiratory Function in Patients with Acute Attack of Bronchial Asthma Complicated with Type 1 Respiratory Failure BAO Jie, QIAO Qingzhe, LU Pei, et al DOI: 10.3969/j.issn.1006-6233.2021.10.026 Objective: To investigate the effects of high-flow nasal cannula (HFNC) and non-invasive positive pressure ventilation (NIPPV) on oxygenation index and respiratory function in patients with acute attack of bronchial asthma complicated with type 1 respiratory failure. Methods: A total of 160 patients with acute attack of bronchial asthma complicated with type 1 respiratory failure who were admitted between December 2017 and December 2019 were enrolled as the research subjects. The patients were divided into observation group and control group according to the simple random sampling of random number table method, with 80 cases in each group. Observation group was treated with HFNC oxygen therapy, and control group was treated with NIPPV oxygen therapy. Before treatment and after 24h of treatment, the oxygenation index (OI) and respiratory function [arterialpartial pressure of oxygen (PaO2), partial pressure of carbon dioxide (PaCO2)]and hemodynamics [heart rate (HR), respiratory rate (RR), mean arterial pressure (MAP)] and comfort [Visual Numerical Score (VNS)] were evaluated in the two groups. The treatment conditions (oxygen therapy time, hospital stay length, invasive ventilation intubation rate) were compared between the two groups. Results: After 24h of treatment, the OI and PaO2 of the two groups were higher than those before treatment (P<0.05), and the increases in observation group were greater than those in control group (P<0.05), and there was no statistically significant difference in PaCO2 between the two groups before and after treatment (P>0.05). After 24h of treatment, the RR, HR, and MAP of the two groups were lower than those before treatment (P<0.05), and the decreases in observation group were greater than those in control group (P<0.05). After 24h of treatment, the comfort VNS scores of both groups were lower than those before treatment (P<0.05), and the decrease in observation group was greater than that in control group (P<0.05). The invasive ventilation intubation rate, oxygen therapy time and hospital stay in observation group were lower than those in control group (P<0.05). Conclusion: HFNC can provide better and more comfortable respiratory support for patients with asthma and type 1 respiratory failure, and has good application value. 2021 Vol. 27 (10): 1705-1709 [Abstract] ( 107 ) HTML (1 KB)  PDF (1241 KB)  ( 300 ) 1710 The Effect of Local Injection of Triamcinolone Acetonide Combined with Semiconductor Laser in the Treatment of Erosive Oral Lichen Planus ZHANG Yongjun, LIU Xiangwei DOI: 10.3969/j.issn.1006-6233.2021.10.027 Objective: To observe the clinical effect of local injection of triamcinolone acetonide combined with semiconductor laser in the treatment of erosive oral lichen planus (EOLP). Methods: In this clinical trial, 62 patients selected according to the inclusion and exclusion criteria were randomly divided into two groups. One group received semiconductor laser therapy (300 MW, 2 times/day, 5 minutes/time) combined with local injection of triamcinolone acetonide (5mL∶ 50mg, once a week), while the control group was treated with triamcinolone acetonide alone, with the same dose and method as the experimental group. Before treatment and 2 weeks and 4 weeks after treatment, the clinical effects of patients with lesion size and pain were measured, recorded and statistically analyzed. Results: 2 weeks and 4 weeks after treatment, the lesion area and pain degree of the treatment group and the control group were significantly reduced. At 2 weeks, the average lesion and pain degree of the treatment group was less than that of the control group, but the difference was not statistically significant. At 4 weeks after treatment, the average lesion and pain degree of the treatment group were further less, and significantly less than that of the control group (P<0.05).Conclusion: The effect of triamcinolone acetonide combined with semiconductor laser in the treatment of erosive oral lichen planus is significant, which is better than that of triamcinolone acetonide alone. 2021 Vol. 27 (10): 1710-1712 [Abstract] ( 88 ) HTML (1 KB)  PDF (1205 KB)  ( 215 ) 1713 Investigation and Analysis of Vitamin D Intake in Adult Population for Health Examination in Huairou, Beijing WANG Xuelian, et al DOI: 10.3969/j.issn.1006-6233.2021.10.028 Objective: To investigate vitamin D intake in the adult population for health examination in Huairou District,Beijing. Methods: 852 healthy local residents were selected and their vitamin D intake from September to October 2020 was analyzed. Results: Vitamin D deficiency in healthy adults in Huairou,Beijing was 24.18%; insufficiency, 61.50%; adequacy, 14.32. The levels of vitamin D intake in middle-aged men were higher than women (P<0.05).There was a significant difference between men in different age brakets as for vitamin D intake(P<0.01). Conclusion: Vitamin D intake in healthy adults in Huairou area of Beijing is deficient. It is important to detect and supplement vitamin D regularly for disease prevention. 2021 Vol. 27 (10): 1713-1715 [Abstract] ( 106 ) HTML (1 KB)  PDF (1220 KB)  ( 263 ) 1716 Effect of Tanshinone on Th1/Th2 Cytokines in Children with Henoch-Schonlein Purpura and Curative Effect of Tanshinone QI Haihua, HUANG Zhanqiang, HE Lei, et al DOI: 10.3969/j.issn.1006-6233.2021.10.029 Objective: To investigate the effect of tanshinone on Th1/Th2 cytokines and therapeutic effect in children with Henoch-Schonlein purpura. Methods: Eighty patients diagnosed with Henoch-Schonlein purpura in our hospital were selected and randomly divided into control group and observation group with 40 cases in each group. Both groups were routinely given symptomatic treatments such as anti-allergic and improving vascular fragility. The group of observation was treated with tanshinone for 2 weeks on the basis of routine treatment. Changes in the levels of INF-γ、IL-2、IL-6、IL-10 in the peripheral blood of the two groups of children before and after therapy was observeed, and the clinical efficacy was evaluated. Results: There was no significant difference in the levels of INF-γ、IL-2、IL-6 and IL-10 between the control and observation groups before treatment (P>0.05). After treatment, the levels of INF-γ and IL-2 in the peripheral blood of the two groups were higher than before, and the levels of IL-6 and IL-10 were lower than before (P<0.05). The increase in peripheral blood INF-γ and IL-2 levels in the observation group was better than that in the control group (P<0.05), and the decrease in peripheral blood IL-10 levels in the observation group was better than that in the control group (P<0.05). The decrease of IL-6 in peripheral blood of the observation group was no different than that of the control group (P>0.05). The effective rate of the observation group was significantly higher than that of the control group, and there was a statistical difference (P<0.05). Conclusion: Tanshinone has a certain clinical effect in the treatment of allergic purpura in children, and its mechanism may act by influencing the levels of peripheral blood cytokines to correct the body's Th1/Th2 immune imbalance. 2021 Vol. 27 (10): 1716-1718 [Abstract] ( 85 ) HTML (1 KB)  PDF (1229 KB)  ( 207 ) 1719 Curative Effect of Penciclovir Combined with Aciclovir Ointment on Herpes Zoster and the Changes of Pain Serum IL-2 and IL-6 Levels YANG Yuanfeng, SU Shengxian, XIE Xiaoli DOI: 10.3969/j.issn.1006-6233.2021.10.030 Objective: To explore the curative effect of penciclovir combined with aciclovir ointment on herpes zoster and the changes of pain, serum interleukin-2 (IL-2) and interleukin-6 (IL-6) levels. Methods: A total of 142 patients with herpes zoster admitted to the hospital between September 2016 and October 2020 were enrolled as the research objects. They were divided into observation group and control group by simple random grouping method, 71 cases in each group. The control group was treated with routine ointment, while observation group was additionally treated with penciclovir. The curative effect and improvement time of clinical symptoms [scabbing time of herpes zoster, stopping development time of blisters, disappearance time of pain], scores of Visual Analogue Scale (VAS) before and after treatment, changes in levels of serum inflammatory factors [IL-2, IL-6, interleukin-10 (IL-10)] and T cell subsets [T lymphocyte (CD3+), Helper T cells (CD4+), Cytotoxic T cells (CD8+), CD4+/CD8+] before and after treatment were compared between the two groups. The incidence of adverse reactions during treatment was recorded. Results: At 7d after treatment, the response rate of treatment in observation group was higher than that in control group (P<0.05). The improvement time of clinical symptoms in observation group was shorter than that in control group (P<0.05). After treatment, VAS scores in both groups were decreased, which were lower in observation group than control group (P<0.05). After treatment, levels of IL-2 and IL-10, CD3+, CD4+ and CD4+/CD8+ in both groups were increased, which were higher in observation group than control group (P<0.05), while IL-6 level was decreased, which was lower in observation group than control group (P<0.05). There was no significant difference in the incidence of adverse reactions during treatment between the two groups (P>0.05). Conclusion: Curative effect of penciclovir combined with aciclovir ointment is significant on herpes zoster, it can relieve neuropathic pain, shorten disappearance time of symptoms, regulate inflammatory factors, and improve immune function, with high safety. 2021 Vol. 27 (10): 1719-1723 [Abstract] ( 112 ) HTML (1 KB)  PDF (1256 KB)  ( 189 ) 1724 Effects of Dipyridamole Adjuvant Therapy on Platelet-Related Parameters Immune Function, CRP and PCT Levels in Children with KD WANG Fengjuan, XING Yaming DOI: 10.3969/j.issn.1006-6233.2021.10.031 Objective: To observe the efficacy of dipyridamole in treating KD, and its effects on platelet related parameters, immune function, serum CRP and PCT. Methods: 68 children with KD admitted to our hospital from February 2019 to February 2021 were prospectively selected as the study subjects. All the children were divided into 2 groups with 34 cases in each group by simple numerical random table method. The control group underwent conventional treatment, and the observation group underwent dipyridamole. Clinical indexes, platelet-related parameters (PLT, WBC, ESR), serum CRP, PCT levels, and immune function indexes (CD3+, CD4+, CD8+, IgA, IgG, IgM) were compared. Results: The subside time of fever, mucosal congestion, limb swelling, lymph node enlargement symptoms and the length of hospital stay in the observation group were significantly lower than those in the control group (P<0.05). The incidence of CAL in the observation group was 14.71%, which was significantly lower than 38.24% in the control group (P<0.05). After treatment, blood PLT, WBC, ESR, CRP and PCT in 2 groups were significantly lower than those before treatment (P<0.05). After treatment, blood PLT, WBC, ESR, CRP and PCT in observation group were significantly lower than those in control group (P<0.05). The differences of blood PLT, WBC, ESR, CRP and PCT in observation group were higher than those in control group before and after treatment (P<0.05). After treatment, CD4+ was significantly lower than before treatment (P<0.05), while CD8+, IgA and IgG were significantly higher than before treatment (P<0.05). After treatment, CD4+ in observation group was significantly lower than that in control group (P<0.05), and CD8+, IgA and IgG in observation group were significantly higher than those in control group (P<0.05). The differences of CD3+, CD4+, CD8+, IgA, IgG, IgM in the observation group were higher than those in the control group (P<0.05). There was no significant difference in the total incidence of adverse reactions between the two groups (P>0.05). Conclusion: Dipyridamole can improve anti-platelet aggregation ability, enhance immune function, and reduce serum CRP and PCT levels in children with KD, finally it achievs the effect of promoting rehabilitation efficiency and reduces the incidence of CAL. 2021 Vol. 27 (10): 1724-1728 [Abstract] ( 96 ) HTML (1 KB)  PDF (1264 KB)  ( 148 ) 1729 Efficacy of Enalapril Maleate Folic Acid Tablets in the Treatment of Elderly H-Type Hypertension with Acute Cerebral Infarction and Its Influence on Levels of SAA LP(a) NT-ProBNP and Hcy ZENG Yuhong, XIAO Yin, CHEN Lin DOI: 10.3969/j.issn.1006-6233.2021.10.032 Objective: To observe the efficacy of enalapril maleate folic acid tablets in the treatment of elderly H-type hypertension with acute cerebral infarction (ACI) and the influence on serum amyloid A (SAA), lysophosphatidic acid [LP(a)], N-terminal pro-B-type natriuretic peptide (NT-proBNP) and homocysteine (Hcy). Methods: Totally 153 elderly patients with H-type hypertension complicated with ACI treated in our hospital between October 2018 and October 2020 were selected as observation subjects, and they were classified into observation group (n=79) and control group (n=74) according to the random number table method. Patients in the control group were given conventional therapy, and patients in the observation group were additionally treated with enalapril maleate folic acid tablets based on the control group. The clinical efficacy of patients was compared, and the blood pressure, serum-related indexes [SAA, LP(a), NT-proBNP, Hcy], cognitive function [Mini-Mental State Examination (MMSE)], neurological function [National Institutes of Health Stroke Scale (NIHSS)] and self-care ability of daily living [Barthel Index (BI)] were observed in the two groups before treatment and 3 months after treatment. Results: The total clinical effective rate was significantly increased in observation group compared with that in control group (97.47% vs 89.19%), and the basic recovery rate was significantly higher than that in control group (P<0.05). 3 months after treatment, the blood pressure level of the two groups was obviously reduced compared to before treatment, and the reduction was more significant in observation group, and was closer to the normal level (P<0.05). The levels of SAA, LP(a), NT-proBNP and Hcy and score of NIHSS in both groups were lower than those before treatment, and the decreases in observation group were more obvious (P<0.05). The scores of AMSE and BI were risen in two groups of patientsthan those before treatment, and observation group had marked increases (P<0.05). Conclusion: Enalapril maleate folic acid tablets for elderly H-type hypertension with ACI has a significant effect in improving the blood pressure level, reducing levels of SAA, LP(a), NT-proBNP and Hcy, improving neurological function, and enhancing cognitive function and self-care ability of daily living. 2021 Vol. 27 (10): 1729-1733 [Abstract] ( 131 ) HTML (1 KB)  PDF (1262 KB)  ( 214 ) 1734 Effect of Butylphthalide Soft Capsules Combined with Dual Antiplatelet Therapy in the Treatment of Acute Ischemic Stroke and the Influence on Serum β2-MG HCY CysC and Neurological Function ZHU Jin, DU Yuping DOI: 10.3969/j.issn.1006-6233.2021.10.033 Objective: To investigate the effect of butylphthalide soft capsules combined with dual antiplatelet therapy in the treatment of acute ischemic stroke and the influence on serum β2-microglobulin (β2-MG), homocysteine (HCY), cystatin C (CysC) and neurological function. Methods: From January 2017 to December 2019, 157 patients with acute ischemic stroke were selected and simple randomly divided into combined group (77 cases) and control group (80 cases). Both groups were treated with aspirin and clopidogrel after thrombolysis. On this basis, the combined group were treated with butylphthalide soft capsules. A month later, the clinical effect was evaluated, and serum β2-MG, HCY and CysC levels were measured. The National Institutes of Health Stroke Scale (NIHSS) scores and Barthel index were used to evaluate. Results: After 1 month of treatment, the total response rate in the combined group was 93.51%, higher than 81.25% in the control group (P<0.05). The difference before and after treatment of serum β2-MG, HCY and CysC in the combined group were higher than those in the control group (P<0.05). The combined group had lower NIHSS scores and higher Barthel index than the control group (P<0.05). The incidences of adverse reactions in the two groups were 10.39% and 12.50%, respectively (P>0.05). Conclusion: On the basis of conventional dual antiplatelet therapy for acute ischemic stroke, combined use of butylphthalide soft capsules can significantly improve the curative effect and stroke-related biochemical indicators, and promote neurological function recovery, thereby increase clinical benefits. 2021 Vol. 27 (10): 1734-1738 [Abstract] ( 102 ) HTML (1 KB)  PDF (1295 KB)  ( 288 ) 1739 Application Effect of Pressing Needle in the Treatment of Tourette Syndrome in Children with Wind Phlegm Obstructing Collaterals FENG Qiuju, CHEN Chen, DU Juan, et al DOI: 10.3969/j.issn.1006-6233.2021.10.034 Objective: To study the effect of pressing acupuncture in the treatment of children with multiple tics of wind-phlegm obstructing collaterals. Methods: This study is a prospective randomized controlled trial. The trial period is from March 2019 to March 2020. The subjects included 120 patients with wind-phlegm-resistance-type TS who were admitted to the Pediatric Clinic of the Second Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine. Random number table method is used in dividing the patients into control group 1, control group 2 and treatment group, 40 cases in each group. During the clinical investigation, 9 cases stopped responding, and 111 cases were actually followed up, including 37 cases in the control group 1, 36 cases in the control group 2, and 38 cases in the treatment group. The control group 1 was treated with traditional acupuncture, once a day, 5 days a week for 5 days; the control group 2 was treated with tiapride hydrochloride, with initial dose of 1/3~1 tablet/time and 1/2~2 tablet/time after 2 weeks of treatment, bid; the treatment group was treated with pressing needles, embedded for 5 days, and rested for 2 days afterwards, before the next needle embedding was performed. The three groups were treated for 8 weeks. Before treatment and 8 weeks after treatment, the three groups of TCM syndrome scores were assessed according to the "Standards for the Diagnosis and Efficacy of TCM Diseases", the Yale Comprehensive Tic Severity Scale (YGTSS) was used to assess the degree of tics in the three groups, and the three groups of serum dopamine (DA) and Norepinephrine (NE), and the efficacy of the three groups after 8 weeks of treatment was determined based on the changes in the YGTSS score. Measurement data such as TCM syndrome score, YGTSS score and serum DA and NE levels were compared by analyzing variance among multiple groups. Further pairwise comparisons between groups were performed by SNK-q test. Paired t-test was used before and after treatment within the group; Chi-square test was used for curative effect. Results: After 8 weeks of treatment, the TCM syndrome scores, YGTSS scores, and serum DA and NE levels in the three groups were all reduced compared with those before treatment (P<0.05), and the above indicators in the treatment group and the control group 1 were significantly lower than those in the control group 2 (P<0.05), there is also a significant difference between the treatment group and the control group 1 (P<0.05); the total effective rate of the treatment group is significantly higher than that of the control group 1 and 2 (P<0.05). Conclusion: The therapeutic effect of pressing needle for wind-phlegm blocking-collateral TS is ideal, which can effectively adjust the levels of serum DA and NE, significantly reduce the score of TCM syndrome, and significantly improve tic symptoms. 2021 Vol. 27 (10): 1739-1743 [Abstract] ( 201 ) HTML (1 KB)  PDF (1265 KB)  ( 421 ) 1757 Research on Safety Regulatory Problems and Countermeasures of Online Meal Ordering XIN Mingxia, LIU Xiaohong, YANG Chao, et al DOI: 10.3969/j.issn.1006-6233.2021.10.039 Objective: To construct the system of supervising and controlling food security with full traceability of online meal ordering. Methods: An investigation on internet meal ordering service system and the status of food safety online catering operators were conducted referring to food safety standards. And the results were analyzed statistically. Results: Contemporary comparison on online meal ordering in Chengde during recent 3 years revealed an increasing monthly average in transaction volume, the difference was statistically significant(30.0%vs41.7%,P<0.05). Statistically significant differences were also revealed in order number (13.3%vs21.4%, P<0.05) , online retails(22.0%vs30.3%), the number of food delivering staff(14.2%vs22.6%,P<0.05),the proportion of online catering operators in catering operators(30.1%vs38.0vs48.4,P<0.05), quantitative reputation grade of catering food safety(20.0%vs33.0%vs42.0, P<0.05), the training rate of food safety regulations(25%vs98%,P<0.05), online catering food operators(12%vs78.8%,P<0.05),the proportion of illegal cases of online catering operators in all catering operators in Chengde city(18.6%vs30.4%vs39.2%,P<0.05). Conclusion: It's effective to supervise and control food security of online meal ordering and demand for full traceability with food safety laws, regulations and standards being strengthened by using information technology. 2021 Vol. 27 (10): 1757-1760 [Abstract] ( 124 ) HTML (1 KB)  PDF (1217 KB)  ( 170 )

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