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2021 Vol. 27, No. 5 Published: 2021-05-31 705 Expression of UHRF1 in Triple-negative Breast Cancer Cells and Its Effect on Proliferation and Invasion YUAN Huiling, WU Lihua, CHEN Guilin, et al DOI: 10.3969/j.issn.1006-6233.2021.05.01 Objective: To study the expression of UHRF1 in triple negative breast cancer (TNBC) breast tissue and its cell line and its effect on cancer cell proliferation and invasion. Methods: Immunohistochemistry was used to detect the expression of UHRF1 in breast tissues of non-TNBC and TNBC patients; immunofluorescence was used to detect the localized expression of Ki67 and UHRF1 in breast tissues of non-TNBC and TNBC patients; qRT-PCR and Western blot were used to detect untransformed human breast epithelial cells (MCF-10A), estrogen and progesterone receptor (ER+ / PR+) breast cancer cell lines (MCF-7) and TNBC breast cancer cell lines (MDA-MB-231 and MDA-MB-436) UHRF1 mRNA and protein expression ;UHRF1-shRNA lentivirus was used to transduce TNBC cell lines (MDA-MB-231 and MDA-MB-436) and the UHRF1 knock down (Knock down, UHRF1 KD) cell line was constructed; Ki67 staining and CCK-8 were used to detect WT and UHRF1 KD cell proliferation and viability changes; Transwell was used to detect WT and UHRF1 KD cell invasion changes; qPCR was used to detect the expression of FOXO4, PPARG, P53 and P21 mRNA. Results: The expression of UHRF1 in breast tissue of TNBC patients was significantly higher than that in non-TNBC patients (P<0.05); the ratio of UHRF1+Ki67+/Ki67+ cells in breast tissue of TNBC patients was significantly higher than that in non-TNBC patients (P<0.05); TNBC cell line ( MDA-MB-231 and MDA-MB-436) UHRF1 mRNA and protein expression were significantly higher than human breast epithelial cells (MCF-10A) and estrogen and progesterone receptor (ER+ / PR+) breast cancer cell lines (MCF- 7) (P<0.05); compared with WT's TNBC cell line (MDA-MB-231 and MDA-MB-436), UHRF1 KD's TNBC cell line (MDA-MB-231 and MDA-MB-436) cells Proliferation, vitality and invasion ability were significantly reduced (P<0.05); UHRF1 KD TNBC cell lines (MDA-MB-231 and MDA-MB-436) FOXO4, PPARG, P53 and P21 mRNA expression were significantly reduced (P<0.05) . Conclusion: UHRF1 is highly expressed in TNBC breast tissues and cell lines, and down-regulation of UHRF1 can inhibit the proliferation and invasion of TNBC cells, which is related to the inhibition of FOXO4/P53 and PPARG/P21 signaling pathways. 2021 Vol. 27 (5): 705-710 [Abstract] ( 160 ) HTML (1 KB)  PDF (2691 KB)  ( 230 ) 710 Mechanism of Isoliquiritigenin in the Treatment of Atopic Dermatitis in Mice Through JAK-STAT Pathway LI Yuanyuan, PAN Xinfeng, CHI Liqiao DOI: 10.3969/j.issn.1006-6233.2021.05.02 Objective: To investigate the mechanism of isoliquiritigenin in the treatment of atopic dermatitis in mice through Janus kinase-signal transducer and activator of transcription(JAK-STAT) pathway. Methods: Sixty SPF male C57BL/6 mice were randomly divided into negative control group, model group, isoliquiritigenin low dose group (50 mg/kg), isoliquiritigenin high dose group (100 mg/kg) and positive control group (prednisolone, 10 mg/kg), with 12 mice in each group. In addition to the negative control group, 2,4-dinitrochlorobenzene (DNCB) was smeared on the back depilated area and ears of mice in other groups to prepare atopic dermatitis (AD) model. On the first day after successful modeling, mice in isoliquiritigenin low dose group, isoliquiritigenin high dose group and positive control group were given corresponding drugs by gavage, while mice in negative control group and model group were given the same amount of distilled water once a day. After 14 consecutive days, the mice were evaluated for scratching behavior and dermatitis score, and serum interleukin-4(IL-4), tumor necrosis factor-α(TNF-α) and immunoglobulin E(IgE) levels, the levels of JAK1, JAK3, STAT3 and STAT6 protein in the skin tissue were detected. Results: The skin of mice in negative control group was normal; the skin of mice in the model group showed varying degrees of erythema, dryness, scratches, epidermal erosion and shedding, and scabs; the symptoms of the isoliquiritigenin low and high dose groups were significantly alleviated, and the isoliquiritigenin high dose group had obvious curative effect; the positive control group has better efficacy than that in the isoliquiritin high dose group. Compared with the negative control group, the number of scratches, dermatitis score, IL-4, TNF-α, IgE, JAK1, JAK3, STAT3 and STAT6 protein in the other groups were increased (P<0.05). Compared with the model group, the number of scratches, dermatitis score, IL-4, TNF-α, IgE, JAK1, JAK3, STAT3 and STAT6 protein in the isoliquiritin low dose group, isoliquiritin high dose group and the positive control group decreased, and the effect in the isoliquiritin dose groups was dose-dependent, but the effect was not as good as the positive control group (P<0.05). Conclusion: Isoliquiritin has therapeutic effect on the skin lesions of AD model mice, and its mechanism may be related to the inhibition of JAK-STAT pathway. 2021 Vol. 27 (5): 710-715 [Abstract] ( 85 ) HTML (1 KB)  PDF (1509 KB)  ( 141 ) 715 sRAGE Inhibiting Mice with Allergic Rhinitis Induced Ovalbumin Via Nitric Oxide Pathway SUI Liping, LI Qinghua, LI Jian, et al DOI: 10.3969/j.issn.1006-6233.2021.05.03 Objective: To investigate the effect of sRAGE on ovalbumin induced allergic rhinitis in mice and the effect of nitric oxide. Methods: 18 female Balb/c mice were divided into control group, allergic rhinitis and sRAGE intervention group. The model of allergic rhinitis was prepared. The mice were sensitized by intraperitoneal injection and nasal drops of ovalbumin. SRAGE, 100ug/ mouse was injected intraperitoneally before and 12 hours after the last time of OVP rhinoplasty. The expression of IL-4 and IFN - γ mRNA was detected by real-time fluorescent quantitative polymerase chain reaction (QRT PCR), and the protein expression of iNOS was detected by Western blot. Results: Compared with the control group, the number of pruritus was significantly increased, the mRNA level of IL-4 and the protein expression of iNOS were significantly increased, and the mRNA level of IFN - γ was decreased in the allergic rhinitis group; Compared with the allergic rhinitis group, the mRNA level of IL-4 and the protein expression of iNOS were significantly decreased, and the mRNA level of IFN - γ was increased. Conclusion: The changes of IL-4, IFN - γ and iNOS levels suggest that sRAGE may mediate the anti allergic effect of sRAGE. 2021 Vol. 27 (5): 715-718 [Abstract] ( 111 ) HTML (1 KB)  PDF (1372 KB)  ( 278 ) 719 Effects of Astragalus Injection Combined with Atorvastatin on Pulmonary Fibrosis and Leukocyte Adhesion Function in Rats with Acute Lung Injury ZHOU Yi, LI Huoping, XIA Guangming, et al DOI: 10.3969/j.issn.1006-6233.2021.05.04 Objective: To study the effects of astragalus injection combined with atorvastatin on pulmonary fibrosis and cell adhesion function in rats with acute lung injury.Methods: 60 wistar rats were randomly divided into sham operation group (control group),model group,atorvastatin group (statin group),Astragalus group and astragalus group combined atorvastatin group (combination group),with 12 rats in each group.The pulmonary fibrosis model of acute lung injury was established.After the beginning of the experiment,the Astragalus group and the combined group were intraperitoneally injected with 1.0ml·kg-1·d-1 astragalus injection every day,and the statin group and the combined group were given atorvastatin 20 mL ·kg-1·d-1 gavage.At the same time,the control group,model group and statin group were given intraperitoneal injection of the same amount of normal saline.The control group,model group and Astragalus group were given the same amount of normal saline.The rats in each group were sacrificed 6h after administration on day 14 for histopathological observation and scoring,and the expression changes of Hydroxyproline (HyP),tumor necrosis factor (TNF-α),intercellular adhesion molecule (ICAM-1),vascular intercellular adhesion molecule (VCAM-1) and platelet endothelial adhesion molecule (PECAM-1) in lung tissues of each group were detected.Results: Compared with the control group,HyP,TNF-α,ICAM-1,VCAM-1 and PECAM-1 expression,alveolitis and pulmonary fibrosis scores of the other four groups were significantly enhanced (P<0.05).Compared with the model group,HyP,TNF-α,ICAM-1,VCAM-1 and PECAM-1 in the lung tissues of the statins group,the Astragalus group and the combination group were significantly lower (P<0.05),and the roots of the combination group were significant.Conclusion: The expressions of ICAM-1,VCAM-1,TNF-α,PECAM-1 and HyP in the pulmonary fibrosis tissues with acute lung injury were significantly unbalanced,while the astragalus,statins and the combined group were able to significantly correct their imbalances,reduce alveolitis and pulmonary fibrosis scores,and thus effectively slow down the occurrence and development of pulmonary fibrosis with acute lung injury.Moreover,the combined group was more effective.. 2021 Vol. 27 (5): 719-723 [Abstract] ( 171 ) HTML (1 KB)  PDF (1874 KB)  ( 253 ) 723 The Influence of TNF-α Antagonist on the Curative Effect of Hormone Combined with Cyclophosphamide in the Treatment of Rheumatoid Arthritis with Interstitial Lung Disease CHEN Xuyan, WANG Qingqing, et al DOI: 10.3969/j.issn.1006-6233.2021.05.05 Objective: To explore new treatment options for rheumatoid arthritis with interstitial lung disease (RA-ILD) in consideration of the poor efficacy of immunosuppressive therapy. Methods: Comparing the erythrocyte sedimentation rate (ESR) of 58 patients with rheumatoid arthritis without interstitial lung disease (RA-NILD) and 30 patients with RA-ILD, as well as levels of C-reactive protein (CRP), C3, C4, rheumatoid factor (RF), CCP antibody (CCP-Ab), TNF-α, IFN-γ, TGF-β, IL-12, IL-2, IL-4, IL-13, IL-17, MCP-1 and KL-6 in serum, and the relationship between various indicators and the incidence of RA-ILD were explored. With TNF-α antagonist combined with prednisone and cyclophosphamide or prednisone combined with cyclophosphamide for the treatment of RA-ILD, these cytokines were tested again to explore the effectiveness of golimumab for the treatment of RA-ILD. Results: The results showed that serum levels of TGF-β, TNF-α, CCP-Ab, IL-2, IL-17, KL-6 and MCP-1 were significantly higher in patients with RA-ILD than those in patients with RA-NILD. In addition, the negative conversion rate of these indicators in serum of patients treated with TNF-α antagonist combined with prednisone and cyclophosphamide was significantly higher than that of patients treated with prednisone combined with cyclophosphamide. Conclusion: TNF-α antagonist optimizes the therapeutic effect of prednisone combined with cyclophosphamide, and provides a new direction for the treatment of RA-ILD patients. 2021 Vol. 27 (5): 723-727 [Abstract] ( 83 ) HTML (1 KB)  PDF (1838 KB)  ( 215 ) 727 Application of Urine MA Combined with Serum TRF α1-MG and Cystatin C in the Diagnosis of Early Kidney Injury of Diabetes Mellitus WANG Tao, GAO Zhiqi, GAO Zhirong, et al DOI: 10.3969/j.issn.1006-6233.2021.05.06 Objective: To explore the application value of urine microalbumin (MA) combined with serum transferrin (TRF), α1-microglobulin (α1-MG) and cystatin C in the diagnosis of early kidney injury of diabetes mellitus. Methods: 122 patients with diabetes mellitus hospitalized between October 2018 and January 2020 were selected and divided into group A (54 case with early kidney injury) group B (68 cases without early kidney injury). Fifty healthy people who completed physical examination in the same period were enrolled as control group (group C). The middle segment urine of all subjects was collected to measure the urine MA level, and venous blood was collected to determine serum TRF, α1-MG and cystatin C levels. The positive detection rates of above indexes were compared among groups. The diagnostic efficiency of all indexes for early kidney injury of diabetes mellitus was analyzed by ROC curves. Results: There were significant differences in urine MA, serum TRF, serum α1-MG, serum cystatin C levels and positive detection rate among groups A, B and C (F=229.053, P＜0.001; F=76.828, P＜0.001; F=87.632, P＜0.001; F=47.151, P＜0.001;χ2=91.652, P＜0.001;χ2=69.050, P＜0.001;χ2=75.260, P＜0.001;χ2=78.732, P＜0.001). The sensitivities and specificities of urine MA, serum TRF, α1-MG and cystatin C for diagnosis of early kidney injury of diabetes mellitus were (95.40%, 81.50%, 75.40%, 81.50%) and (80.70%, 66.70%, 87.70%, 89.50%), respectively. The sensitivity and specificity of their combined diagnosis were 96.92% and 49.12% (AUC=0.900, 95%CI=0.846-0.954). Conclusion: The combined detection of urine MA, serum TRF, α1-MG and cystatin C is of high diagnostic value and high accuracy for early kidney injury of diabetes mellitus, which is of great significance for early screening and prevention of diabetic nephropathy. 2021 Vol. 27 (5): 727-732 [Abstract] ( 102 ) HTML (1 KB)  PDF (1413 KB)  ( 157 ) 732 Effects of Iguratimod on Immune Function and Serum RF Anti-CCP and Wnt-3α in Patients with Rheumatoid Arthritis LIU Ying, LIN Shudian, PAN Chuying, et al DOI: 10.3969/j.issn.1006-6233.2021.05.07 Objective: To observe the application effects of iguratimod on rheumatoid arthritis (RA) and its effects on immune function, serum indexes and functional status of patients. Methods: A total of 156 patients with active RA admitted from January 2019 to June 2020 were selected and divided into observation group and control group according to the odd and even numbers of hospitalization order, with 78 cases in each group. Control group was given oral methotrexate (once for 10mg, once/week), and observation group was given oral iguratimod (once for 25 mg, twice/day) on the basis of control group, and they were continuously treated for 12 weeks. The 28 joint disease activity score (DAS28) and treatment response were compared between the two groups, and the levels of immune function indicators (helper T cells Th1 and Th17 and regulatory T cells) and serum indicators [C-reactive protein (CRP), rheumatoid factor (RF), anti-cyclic citrullinated peptide antibody (anti-CCP), secreted glycoprotein (Wnt)-3α] were analyzed before and after treatment, and Health Assessment Questionnaire (HAQ) was used to evaluate the functional status of patients, and adverse drug reactions were recorded. Results: 12 weeks after treatment, the DAS28 value of the two groups was significantly lower than that before treatment (P<0.05), and the decrease in observation group was greater than that in control group (P<0.05). The total response rate in observation group was significantly higher than that in control group (97.44% vs 88.46%) (P<0.05). The percentages of Th1 and Th17 in the two groups were significantly lower than those before treatment (P<0.05) while the percentage of regulatory T cells was significantly higher than that before treatment (P<0.05), and the decreases of percentages of Th1 and Th17 in observation group were greater than those in control group (P<0.05), and the increase of percentage of regulatory T cells was greater than that in control group (P<0.05). The levels of serum CRP, RF and anti-CCP and expression level of Wnt-3α in the two groups were significantly lower than those before treatment (P<0.05), and the decreases of levels of serum CRP, RF and anti-CCP and expression level of Wnt-3α in observation group were greater than those in control group (P<0.05). The HAQ score of the two groups was significantly lower than that before treatment (P<0.05), and the decrease of HAQ score in observation group was greater than that in control group (P<0.05). There was no significant difference in the incidence rate of adverse reactions between observation group and control group (10.26% vs 6.41%) (P>0.05). Conclusion: Iguratimod in the treatment of RA can improve the response rate of treatment, help regulate the immune function, relieve the inflammation level, and promote the recovery of functional status, with good safety. 2021 Vol. 27 (5): 732-737 [Abstract] ( 99 ) HTML (1 KB)  PDF (1277 KB)  ( 275 ) 737 The Relationship Between Serum GGT CysC and Hs-CRP Levels and Prognosis in Patients with Corpulmonale Complicated by Respiratory Failure LIU Rui, LIU Mei, PENG Liqing, et al DOI: 10.3969/j.issn.1006-6233.2021.05.08 Objective: To investigate the relationship between serum γ-glutamyl transpeptidase (GGT), cystatin C (CysC) and high-sensitivity C-reactive protein (hs-CRP) levels and prognosis in patients with corpulmonale complicated by respiratory failure. Methods: 95 patients with corpulmonale complicated by respiratory failure (observation group) treated in the hospital between February 2017 and January 2020, and 50 healthy subjects (control group) received physical examination in this hospital during the same period were enrolled in this study. Serum GGT, CysC and hs-CRP levels in the two groups were detected and compared. According to the condition during hospitalization and within 24 hours after discharge, the patients were divided into survival group and death group. Multivariate logistic regression analysis of relevant factors affecting the patients’ prognosis was performed. Results: Serum GGT, CysC and hs-CRP levels in the observation group were higher than those in the control group (P<0.05). Serum GGT, CysC and hs-CRP levels were higher in the death group than in the survival group (P<0.05). Serum GGT, CysC and hs-CRP were influencing factors of death in patients with corpulmonale complicated by respiratory failure (P<0.05). Conclusion: Serum GGT, CysC and hs-CRP levels in patients with corpulmonale complicated by respiratory failure are relatively higher, which are correlated with the prognosis. Clinically, the prognosis can be assessed by monitoring serum GGT, CysC and hs-CRP levels in patients. 2021 Vol. 27 (5): 737-741 [Abstract] ( 111 ) HTML (1 KB)  PDF (1243 KB)  ( 303 ) 742 Glucocorticoid Combined with Bronchoalveolar Lavage Fluid on Children with Lobar Pneumonia and Its Influences on Plasma T Lymphocytes Immunoglobulins and Inflammatory Factors TANG Yafei, JI Kaituo, MA Yong DOI: 10.3969/j.issn.1006-6233.2021.05.09 Objective: To explore the curative effect of glucocorticoid combined with bronchoalveolar lavage fluid (BALF) on children with lobar pneumonia and its influences on plasma T lymphocytes, immunoglobulins and inflammatory factors. Methods: A total of 158 children with lobar pneumonia who were admitted to the hospital from October 2018 to October 2019 were enrolled and divided into observation group (n=83) and control group (n=75) by simple random grouping method. The control group was treated with glucocorticoid, while observation group was treated with glucocorticoid and BALF. The clinical curative effect, improvement of clinical symptoms and signs, levels of plasma T lymphocytes, immunoglobulins and inflammatory factors before and after treatment were compared between the two groups. Results: The total response rate of treatment in observation group was higher than that in control group (92.77% vs 78.67%) (P<0.05). The relief time of cough, fading time of hyperpyrexia, disappearance time of lung shadows and lung rales in observation group were shorter than those in control group (P<0.05). After treatment, CD3+ and CD4+ levels in observation group were higher than those in control group, while CD8+ level was lower than that in control group (P<0.05). After treatment, levels of immunoglobulin A (IgA), immunoglobulin M (IgM) and immunoglobulin G (IgG) in observation group were higher than those in control group, while levels of interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) were lower than those in control group (P<0.05). Conclusion: The curative effect of glucocorticoid combined with BALF is significant on children with lobar pneumonia, which can effectively relieve clinical symptoms, improve immune function, reduce inflammatory response and promote their recovery. 2021 Vol. 27 (5): 742-746 [Abstract] ( 95 ) HTML (1 KB)  PDF (1246 KB)  ( 206 ) 746 Serum Levels and Significance of SCD40L TNF-α and MMP-9 in Patients with COPD FU Ying, CAI Xiaoyu, ZHONG Qiao, et al DOI: 10.3969/j.issn.1006-6233.2021.05.010 Objective: To observe the expression levels of serum soluble CD40 ligand (sCD40L), tumor necrosis factor α (TNF-α) and matrix metalloproteinase 9 (MMP-9) in patients with chronic obstructive pulmonary disease (COPD) and analyze their significance. Methods: 82 patients with COPD were selected and divided into acute exacerbation group (n = 46) and clinical remission group (n = 36) according to the clinical staging. Meanwhile, 40 healthy people were made the control group. The expression levels of serum sCD40L, TNF-α and MMP-9 and lung function indexes [forced expiratory volume in 1s (FEV1), forced vital capacity (FVC), FEV1/FVC, the percentage of FEV1 in predicted value (FEV1%)] were compared among the 3 groups, and correlation analysis was performed. Results: The order of expression levels of serum sCD40L, TNF-α and MMP-9 in the 3 groups from high to low was as follows: the acute exacerbation group, the clinical remission group, the control group (P<0.017). The order of FEV1, FVC, FEV1/FVC and FEV1% in the 3 groups from low to high was as follows: the acute exacerbation group, the clinical remission group, the control group (P<0.017). Serum sCD40L, TNF-α and MMP-9 levels were negatively correlated with lung function (FEV1/FVC, FEV1%) in patients with COPD (P<0.05). Conclusion: The expression levels of serum sCD40L, TNF-α and MMP-9 in patients with COPD show an increasing trend, especially in patients with acute exacerbation. The higher the sCD40L, TNF-α and MMP-9 levels, the worse the lung function. The detection of the three indexes can provide an effective basis for the evaluation of COPD. 2021 Vol. 27 (5): 746-751 [Abstract] ( 114 ) HTML (1 KB)  PDF (1844 KB)  ( 471 ) 751 Levels of Serum P-Selectin and VWF in Elderly Patients with Acute Pancreatitis and Their Relationship with Prognosis HUANG Xiangmei, LI Jun, WANG Xuejiao, et al DOI: 10.3969/j.issn.1006-6233.2021.05.011 Objective: To explore the levels of serum P-selectin and vWF in elderly patients with acute pancreatitis (AP) and their relationship with prognosis. Methods: A total of 159 AP patients who were treated in the hospital from June 2016 to June 2019 were enrolled as study group, while another 58 healthy controls during the same period were enrolled as control group. The levels of serum P-selectin and vWF were measured and compared between the two groups. The differences in P-selectin and vWF levels were compared among AP patients with different severity and prognosis. The single factors and independent risk factors that affected prognosis of AP patients were explored. The value of P-selectin, vWF and their combination in predicting prognosis of AP was explored. Results: Compared with control group, levels of serum P-selectin and vWF were significantly increased in study group (P<0.05). With the exacerbation of AP, levels of serum P-selectin and vWF were significantly increased (P<0.05). Compared with survival group, levels of serum P-selectin and vWF were significantly increased in death group (P<0.05). ROC curve analysis showed that AUC of P-selectin combined with vWF for predicting prognosis was significantly higher than that of P-selectin and vWF alone (0.935 vs 0.841, 0.833) (P<0.05). The results of univariate analysis on 28d survival rate of AP patients showed that there were certain effects of drinking history, cholelithiasis, scores of RANSON and BISAP, AP severity, P-selectin and vWF on 28d survival rate of AP patients (P<0.05). The results of Logistic multivariate analysis showed that drinking, cholelithiasis, RASON score not lower than 3 points, BISAP score not lower than 3 points, SAP, P-selection VWF not lower than 64.31μg/mL and vWF not lower than 231.68% were all independent risk factors affecting the 28d survival rate of AP patients (P<0.05). Conclusion: The levels of serum P-selectin and vWF in elderly AP patients are significantly increased, which are closely related to their prognosis. The combination of the two is of good clinical application value in predicting prognosis of AP patients. 2021 Vol. 27 (5): 751-756 [Abstract] ( 96 ) HTML (1 KB)  PDF (1356 KB)  ( 223 ) 757 Effect of Tube Stomach Retrosternal and Transesophageal Bed Pathways on the Success Rate Lung Function and Postoperative Complications of Thoracoscopic Combined with Esophageal Cancer Surgery WANG Xingbang, JIANG Pengpeng, ZHU Siyu DOI: 10.3969/j.issn.1006-6233.2021.05.012 Objective: To explore the effect of the trans-sternal route of tubular stomach and trans-esophageal bed route on the success rate, lung function and postoperative complications of thoracoscopic esophageal cancer surgery. Methods: From January 2019 to June 2020, 60 cases of thoracoscopic combined with esophageal cancer surgery in our hospital were selected and divided into esophageal bed pathway group and retrosternal pathway group according to the simple random number table method, with 30 cases in each group. The treatment success rate, indexes during operation, indexes of pulmonary function 24 hours after operation, postoperative complications and quality of life 7 days after operation were observed. Results: The patients of two groups were successfully completed the operation, the success rate was 100%. There was no death during the perioperative period. There was no statistical difference (P>0.05). There was no significant difference in operation time, intraoperative blood loss, length of hospital stay and indwelling time of drainage tube between the two groups (P>0.05). The gastrointestinal decompression fluid in the esophageal bed pathway group was significantly higher than that in the retrosternal pathway group (P<0.05). FVC and respiratory rate of the two groups were significantly improved after treatment (P<0.05), but there was no significant difference between the two groups (P>0.05). There was no significant difference in anastomotic stenosis, pulmonary infection, arrhythmia and complications between the two groups (P>0.05), but the incidence of anastomotic leakage in the retrosternal pathway group was significantly higher than that in the esophageal bed pathway group (P<0.05). The difference between the two groups was statistically significant (P<0.05), or = e1.159 = 3.150, 95% confidence interval was (e0.174, e2.144) = (0.473, 5.828), suggesting that the incidence of acid reflux heartburn in the esophageal bed pathway group was significantly higher than that in the control group. The time difference was statistically significant (P<0.05), suggesting that the attack rate of patients at different time points had significant difference. The postoperative quality scores of the two groups were significantly improved (P<0.05), but there was no significant difference between the two groups (P>0.05). Conclusion: The results show that the tube stomach retrosternal path and transesophageal bed path had good effect in thoracoscopic combined with esophageal cancer surgery, with high success rate, strong safety, and help to improve the postoperative lung function and quality of life of patients. The former had relatively less gastrointestinal decompression fluid, higher probability of anastomotic leakage, and the latter had more serious gastrointestinal reflux, so it can be selected according to the specific situation Methods individualized treatment. 2021 Vol. 27 (5): 757-762 [Abstract] ( 96 ) HTML (1 KB)  PDF (1262 KB)  ( 263 ) 762 Relationship Between Levels of TGF-β and IFN-γ in Bronchoalveolar Lavage Fluid and Serum and Idiopathic Pulmonary Fibrosis LUO Li, XIONG Yan, et al DOI: 10.3969/j.issn.1006-6233.2021.05.013 Objective: To analyze the relationship between levels of transforming growth factor-β (TGF-β) and interferon-γ (IFN-γ) in bronchoalveolar lavage fluid (BALF) and serum and idiopathic pulmonary fibrosis (IPF). Methods: The clinical data of 135 patients with IPF from February 2016 to December 2019 were retrospectively analyzed. BALF and serum samples were collected within 24 h after admission. According to the modified Medical Research Council (mMRC) dyspnea score, the patients were divided into mild group and moderate-to-severe group, and the levels of TGF-β and IFN-γ in BALF and serum were compared between the two groups, and the correlation between levels of TGF-β and IFN-γ in BALF and serum and the severity of disease was analyzed. 135 patients with IPF were divided into effective group and ineffective group based on the clinical efficacy. Receiver operating characteristic curve (ROC curve) was used to assess the predictive value of levels of TGF-β and IFN-γ in BALF and serum on effective treatment. Results: There were no significant differences in gender, age and course of disease between the two groups (P>0.05). The scores of ground glass opacity and interstitial fibrosis in mild group were lower than those in moderate-to-severe group (P<0.05), and the levels of TGF-β in BALF and serum in mild group were lower than those in moderate-to-severe group (P<0.05) while the levels of IFN-γ in BALF and serum were higher than those in moderate-to-severe group (P<0.05). Pearson correlation analysis showed that TGF-β levels in BALF and serum were significantly positively correlated with scores of mMRC, ground glass opacity and interstitial fibrosis (P<0.05), and IFN-γ levels in BALF and serum were significantly negatively correlated with scores of mMRC, ground glass opacity and interstitial fibrosis (P<0.05). Among 135 patients with IPF, there were 29 cases (21.48%) with ineffective treatment and 106 cases (78.52%) with effective treatment. The levels of TGF-β in BALF and serum in ineffective group were higher than those in effective group (P<0.05) while the levels of IFN-γ in BALF and serum in ineffective group were lower than those in effective group (P<0.05). After ROC curve analysis, it was found that levels of TGF-β and IFN-γ in BALF and serum were effective and beneficial for the treatment and diagnosis of patients with IPF (AUC=0.733, 0.718, 0.735, 0.796, P<0.05), and the cut-off values were 958.42 ng/L, 229.14 ng/L, 1009.37 ng/L and 250.87 ng/L. Conclusion: The levels of early TGF-β and IFN-γ in BALF and serum after admission are not only related to the severity of IPF, but can also assist in predicting the effective treatment status of patients, and they are beneficial for guiding clinical treatment. 2021 Vol. 27 (5): 762-767 [Abstract] ( 132 ) HTML (1 KB)  PDF (1411 KB)  ( 168 ) 767 Effects of Modified Liuwei Dihuang Decoction Combined with Cardiopulmonary Rehabilitation Training on Exercise Tolerance, Serum IL-6 IL-8 and Cys-C Levels in Patients with Stable COPD ZHENG Jun, HUANG Pei, WU Caihua DOI: 10.3969/j.issn.1006-6233.2021.05.014 Objective: To explore the effects of modified Liuwei Dihuang Decoction combined with cardiopulmonary rehabilitation training on exercise tolerance, serum interleukin-6 (IL-6), interleukin-8 (IL-8) and cystatin-C (Cys-C) levels in patients with stable chronic obstructive pulmonary disease (COPD). Methods: 192 patients with stable COPD admitted to the hospital from May 2019 to May 2020 were enrolled and divided into observation group and control group by random number table method, 96 cases in each group. The control group was treated with routine western medicine, while observation group was given modified Liuwei Dihuang Decoction combined with cardiopulmonary rehabilitation training. The clinical curative effect, scores of TCM symptoms, exercise tolerance, pulmonary function, serum IL-6, IL-8 and Cys-C levels before and after treatment were compared between the two groups. Results: The total response rate of clinical treatment in observation group was higher than that in control group (94.79% vs 86.46%) (P<0.05). After treatment, scores of TCM symptoms were significantly decreased, while 6-min walk test (6MWT) was significantly increased in both groups (P<0.05). The difference values of the above two indexes before and after treatment in observation group were higher than those in control group (P<0.05). After treatment, forced vital capacity (FVC), forced expiratory volume in 1 second (FEV1), maximum ventilation per minute (MVV) and FEV1/FVC were significantly increased in both groups (P<0.05). The difference values of the above four indexes before and after treatment in observation group were higher than those in control group (P<0.05). After treatment, levels of IL-6, IL-8 and Cys-C were significantly decreased in both groups (P<0.05). The difference values of the above three indexes before and after treatment in observation group were higher than those in control group (P<0.05). Conclusion: The modified Liuwei Dihuang Decoction combined with cardiopulmonary rehabilitation training can improve the clinical curative effect on patients with stable COPD, improve exercise tolerance, relieve clinical symptoms, promote recovery of pulmonary function, and reduce levels of serum IL-6, IL-8 and Cys-C. 2021 Vol. 27 (5): 767-772 [Abstract] ( 100 ) HTML (1 KB)  PDF (1268 KB)  ( 150 ) 772 Ultrasonic Characteristics and Diagnostic Value of Joint Lesions in Patients with Rheumatoid Arthritis ZHOU Meixia, et al DOI: 10.3969/j.issn.1006-6233.2021.05.015 Objective: To explore the ultrasonic characteristics and diagnostic value of joint lesions in patients with rheumatoid arthritis (RA). Methods: The clinical data of 60 RA patients (observation group) who were admitted to the hospital from January 2018 to January 2020 were retrospectively analyzed. Another 50 healthy people who underwent physical examination in the hospital during the same period were enrolled as control group. All underwent ultrasound examination. The ultrasonic features of joint lesions were observed. The synovial thickness, depth of joint fluid and bursa suprapatellaris fluid, and blood flow index of synovial artery were compared between the two groups. Results: In observation group, there were 54 patients with irregular synovial thickness, whose surface was rough. There were villi-like structures protruding into the effusion synovial cavity. There were 48 cases with blood flow signals and 50 cases with different degree of joint fluid, which was located at bursa suprapatellaris. There were 15 patients with blurred knee articular cartilage edge, cartilages were thinned, and cartilage surface was rough. There was worm-bittenlike damage of cartilage bone in some patients. Depth of bursa suprapatellaris fluid, synovial thickness and depth of joint fluidin observation group were higher than those in control group, while blood flow index of synovial artery was lower than that in control group (P<0.05). The detection rates of intra-synovial blood flow in knee, bursa suprapatellaris fluid and synovial thickening in observation group were 80.00%, 83.33%and 90.00%, respectively. Conclusion: Ultrasound has a high detection rate in early knee joint lesions in RA patients, which can accurately reflect pathological changes, and provide reference for clinical treatment. 2021 Vol. 27 (5): 772-775 [Abstract] ( 100 ) HTML (1 KB)  PDF (1561 KB)  ( 152 ) 775 Changes and Clinical Significance of Serum Adrenocorticotropic Hormone, Cortisol, Atrial Natriuretic Peptide and Interleukin-6 Levels Before and After Pediatric Nephroblastoma Surgery HUANG Qingchen, QI Boxiang, et al DOI: 10.3969/j.issn.1006-6233.2021.05.016 Objective: To explore the changes and clinical significance of serum adrenocorticotropic hormone (ACTH), cortisol (Cor), atrial natriuretic peptide (ANP) and interleukin-6 (IL-6) levels before and after pediatric nephroblastoma surgery. Methods: A total of 113 children undergoing pediatric nephroblastoma surgery who were admitted from February 2016 to August 2020 were enrolled. According to presence or absence of postoperative surgical stress response and complications, they were divided into poor prognosis group (32 cases) and good prognosis group (81 cases). The levels of serum ACTH, Cor, ANP and IL-6 in both groups before surgery, at 1d, 2d and 3d after surgery were detected. Results: The levels of serum ACTH, Cor, ANP and IL-6 at 1d, 2d and 3d after surgery were higher than those before surgery in both groups (P<0.05), which showed increased trend in poor prognosis group (P<0.05), while showed decreased trend in good prognosis group (P<0.05). At 1d, 2d and 3d after surgery, levels of serum ACTH, Cor, ANP and IL-6 in poor prognosis group were higher than those in good prognosis group (P<0.05). Conclusion: Monitoring ACTH, Cor, ANP and IL-6 after pediatric nephroblastoma surgery is beneficial to determine surgical stress degree. Continuously high levels of ACTH, Cor, ANP and IL-6 may indicate poor prognosis. 2021 Vol. 27 (5): 775-779 [Abstract] ( 74 ) HTML (1 KB)  PDF (1256 KB)  ( 218 ) 779 Value of Circulating Tumor Cells and Tumor Markers in Peripheral Blood for Evaluating the Curative Effect on Elderly Patients with Non-Small Cell Lung Cancer After Chemotherapy LI Xiaohua, LI Jie, TANG Junwei DOI: 10.3969/j.issn.1006-6233.2021.05.017 Objective: To explore the value of circulating tumor cells (CTCs) and tumor markers in peripheral blood for evaluating the curative effect on elderly patients with non-small cell lung cancer (NSCLC) after chemotherapy. Methods: A total of 86 elderly NSCLC patients admitted to the hospital from September 2017 to February 2019 and confirmed by pathological examination were enrolled as the research subjects. All underwent chemotherapy with docetaxel and cisplatin. The levels of CTCs in peripheral blood, serum neuron specific enolase (NSE), cytoplasmic thymidine kinase-1 (TK-1) and midkine (MK) were detected before chemotherapy. The clinical curative effect after 6 months of treatment was evaluated. The evaluation value of peripheral blood CTCs, serum NSE, TK-1 and MK for curative effect on elderly NSCLC patients after chemotherapy was analyzed by univariate analysis, multivariate Logistic regression analysis and ROC curve analysis. Results: After chemotherapy, there were 49 cases (56.98%) effective, who were included into effective group, and there were 37 cases (43.02%) ineffective, who were included into ineffective group. After test, levels of serum NSE, TK-1 and MK, and positive rate of CTCs in ineffective group were significantly higher than those in effective group (P<0.05). The positive rate of CTCs was significantly positively correlated with the expression of serum NSE, TK-1 and MK (P<0.05). The results of univariate analysis showed that there were significant differences in TNM staging of tumors, peripheral blood CTCs, serum NSE, TK-1 and MK between the two groups (P<0.05). The results of multivariate regression analysis showed that positive peripheral blood CTCs, serum NSE not lower than 40.0 ng/ml, TK-1 not lower than 4.5 ng/ml and MK not lower than 0.8 ng/ml were independent predictors of ineffective curative effect on elderly NSCLC patients after chemotherapy. The results of ROC curve analysis showed that area under the ROC curve (AUC) of serum NSE, TK-1 and MK alone for predicting ineffective curative effect on elderly NSCLC patients after chemotherapy was lower than that of their combination (P<0.05). Conclusion: The high positive expression rate of peripheral blood CTCs, and high expression of serum NSE, TK-1 and MK are important influencing factors of ineffective curative effect on elderly NSCLC patients after chemotherapy. The combined detection of the three is of good application value in evaluating the curative effect on elderly NSCLC patients after chemotherapy. 2021 Vol. 27 (5): 779-785 [Abstract] ( 98 ) HTML (1 KB)  PDF (1453 KB)  ( 248 ) 785 Clinical Value of Serum HMGB-1 MIF and PDGF in Predicting Short-Term Prognosis of Acute Cerebral Hemorrhage LUO Jinlun, WU Fei, ZHU Dongqiang, et al DOI: 10.3969/j.issn.1006-6233.2021.05.018 Objective: To analyze the clinical value of serum high mobility group protein-1 (HMGB-1), macrophage migration inhibitory factor (MIF) and platelet-derived growth factor (PDGF) in predicting short-term prognosis of acute cerebral hemorrhage. Methods: Clinical data of 106 patients with acute cerebral hemorrhage who were treated in the hospital between May 2016 and May 2020 were retrospectively analyzed. According to the severity of nervous system damage, the patients were divided into mild group (NIHSS 1 ~ 4 points, n=23), moderate group (NIHSS 5 ~ 20 points, n=53) and severe group (NIHSS > 20 points, n=30). Meanwhile, 40 healthy controls were made the control group. Three months later, according to the Glasgow Outcome Score (GOS), the patients were divided into favorable prognosis group (GOS 4～5 points, n=60) and bleak prognosis group (GOS ≤ 3 points, n=46). Serum HMGB-1, MIF and PDGF levels were detected, and their clinical value in predicting short-term prognosis of acute cerebral hemorrhage was analyzed with the area under the ROC curve (AUC). Results: Serum HMGB-1, MIF and PDGF levels were higher in patients with cerebral hemorrhage than the control group (P<0.05), higher in the severe group than the mild and moderate groups, higher in the moderate group than the mild group (P<0.05), and higher in the favorable prognosis group than the bleak prognosis group (P<0.05). The sensitivity and AUC area of serum HMGB-1, MIF and PDGF were higher than those of single detection (P<0.05). Conclusion: Serum HMGB-1, MIF and PDGF have high value in predicting short-term prognosis of acute cerebral hemorrhage, which can be used for treatment guidance. 2021 Vol. 27 (5): 785-789 [Abstract] ( 84 ) HTML (1 KB)  PDF (1392 KB)  ( 154 ) 789 Effects of Intravitreal Injection of Ranibizumab or Conbercept Combined with AGV on Neovascular Glaucoma and Their Influence on VEGF and IL-6 Levels in Aqueous Humor WANG Qian, ZHAO Xiaoxia, WU Juan, et al DOI: 10.3969/j.issn.1006-6233.2021.05.019 Objective: To explore the effects of intravitreal injection of ranibizumab or conbercept combined with ahemd glaucoma valve (AGV) in the treatment of neovascular glaucoma (NVG) and their influence on vascular endothelial growth factor (VEGF) and interleukin- 6 (IL-6) levels in aqueous humor. Methods: A total of 96 patients (96 eyes) with NVG admitted to our hospital from January 2016 to May 2018 were selected, and the clinical data of all patients were retrospectively analyzed. Patients were divided into ranibizumab group (group A, 34 cases, 34 eyes), conbercept group (group B, 33 cases, 33 eyes) and control group (group C, 29 cases, 29 eyes) according to different treatment regimens. The neovascularization regression of iris and chamber angle surface was observed among patients. The intraocular pressure, visual acuity and aqueous humor VEGF and IL-6 levels in the three groups were compared before and after surgery. The incidence rate of postoperative complications was recorded. Results: The neovascularization regression of iris and chamber angle surface was good in groups A and B, and there were still new blood vessels in group C, and the difference among the three groups was significant (P<0.05), and there was no statistical difference between group A and group B (P>0.05). There were statistical significance in the between-group effect, time-point effect and interaction of time-point and between-group of intraocular pressure among the three groups before surgery and at 2 w, 1 month, 3 months and 6 months after surgery (P<0.05). The intraocular pressure in the three groups at 2 w, 1 month, 3 months and 6 months after surgery was significantly decreased compared with that before surgery, and the improvement of visual acuity in group A and group B was better than that in group C at 6 months after surgery (P<0.05), but there were no significant differences in the above indexes between group A and group B (P>0.05). There was no significant difference in visual acuity among the three groups at 6 months after surgery (P<0.05). After treatment, the levels of VEGF and IL-6 in aqueous humor were decreased significantly in the three groups compared with those before treatment (P<0.05), and the comparisons of differences of the above indexes in the three groups before and after treatment were statistically significant (P<0.05). There was no statistically significant difference in the incidence rate of complications among the three groups (P>0.05). Conclusion: Intravitreal injection of ranibizumab or conbercept combined with AGV both have reliable efficacy and safety in the treatment of NVG, and they can effectively reduce intraocular pressure, improve visual acuity, promote neovascularization regression, and inhibit the expression of vasoactive substances and inflammatory factors in aqueous humor. The two drugs have comparable treatment effects. 2021 Vol. 27 (5): 789-794 [Abstract] ( 286 ) HTML (1 KB)  PDF (1273 KB)  ( 377 ) 794 Effect of Different Incision of Phacoemulsification on Ocular Surface of Diabetic Patients SU Qi, ZHANG Xin, ZHAO Yan, et al DOI: 10.3969/j.issn.1006-6233.2021.05.020 Objective: To study the effect of different phacoemulsification incision on ocular surface of diabetic patients. Methods: 120 cases of diabetic patients with cataract in our hospital were selected as the research object, all of them received phacoemulsification. The patients were divided into three groups, 40 cases and 40 eyes in each group. Group A was treated with limbal incision, group B was treated with upper clear corneal incision, and group C was treated with temporal clear corneal incision. Dry eye symptom score, corneal fluorescein staining (FL) score, tear film break-up time (but) and basic tear secretion test (SIT) were evaluated before operation and 1, 7, 14, 30 days after operation. Results: Postoperative dry eye symptom scores increased, BUT shortened, FL score increased, and SIt decreased in all three groups. In addition, postoperative index recovery of group A was significantly better than that of group B and Group C (both P<0.05). Conclusion: In phacoemulsification, corneoscleral incision has little effect on tear film stability and good safety. 2021 Vol. 27 (5): 794-799 [Abstract] ( 111 ) HTML (1 KB)  PDF (1735 KB)  ( 217 ) 799 Observation on the Curative Effect of Partial Intersphincteric Resection for Low Rectal Cancer Under Laparoscopic Full Transabdominal Approach CHEN Qingkuang, ZHAO Zimin, ZHEN Honghong, et al DOI: 10.3969/j.issn.1006-6233.2021.05.021 Objective: To observe the clinical efficacy and quality of life of laparoscopic partial internal sphincter resection for low rectal cancer. Methods: From January 2015 to December 2019, 181 patients were selected and divided into observation group, control group 1 and control group 2 according to different surgical methods. The intraoperative and postoperative recovery, radical tumor resection, anal function and quality of life score were collected. Results: The distance between tumor and anal margin in the observation group was lower than that in control group 1 ( 3.72±0.74cm vs 5.67±0.54cm, P<0.05 ), which was statistically significant. Among the three groups of surgical indicators, the amount of bleeding in the observation group and control group 1 was less than that in control group 2 ( 52.3±28.2ml vs 48.2±20.9 ml vs 96.2±27.3 ml ), the operation time was shorter (197±6.8min vs 195±5.4 min vs 208±10.7 min ), and the postoperative hospitalization time was shorter (13.5±4.9 d vs 13.1±3.7d vs 17.3±4.7d ), with statistical significance ( P<0.05 ). There was no significant difference in postoperative complications among the three groups. There was no significant difference in distal margin distance, circumferential margin and lymph node dissection among the three groups in tumor radical resection. The overall survival rates in the three groups were 93.3 %, 90.2 % and 91.7 %, respectively ( P>0.05 ), with no statistical significance. The comparison of anal function between the two groups showed that the resting pressure of anal canal, maximum systolic pressure of anal canal, maximum tolerated volume and defecation frequency in control group 1 were faster than those in the observation group within 3 months after operation, and the good rate of defecation function was higher ( 85.2 %vs 58.3%, P<0.05 ). However, there was no statistical difference in anal function between 3 and 6 months after surgery ( P>0.05 ).In the quality of life score of tumor patients, the anus-preserving patients in the observation group were significantly higher than those in control group 2 ( P<0.05 ). The gradual recovery of anal function helps to improve the quality of life. Conclusion: Laparoscopic total transabdominal partial internal sphincter resection and anal sphincter preservation for patients with low rectal cancer has good postoperative clinical efficacy and anal function recovery, which is helpful to improve the quality of life of patients and has certain safety and feasibility. 2021 Vol. 27 (5): 799-805 [Abstract] ( 109 ) HTML (1 KB)  PDF (1498 KB)  ( 124 ) 805 Clinical Efficacy of Isokinetic Combined with Proprioceptive Training in the Treatment of Knee Osteoarthritis JIA Wenping, ZANG Chuanyan, ZHANG Hongqian, et al DOI: 10.3969/j.issn.1006-6233.2021.05.022 Objective: To explore the effect of isokinetic exercise combined with proprioceptive training on knee flexion and extensor strength, coordination and limb balance function in patients with knee osteoarthritis. Methods: 60 patients with knee osteoarthritis were selected from the Department of Rehabilitation Medicine, Qingdao Binhai University affiliated Hospital. The patients were randomly divided into 3 groups (n = 20). Group A was treated with physical factor therapy and routine exercise therapy, group B was treated with physical factor therapy and isokinetic concentric combined eccentric muscle strength training, and group C was treated with proprioceptive training on the basis of group B. The peak torque (PT), the ratio of flexion to extensor force (H/Q) and the active angle reappearance value (AAR) were measured by isokinetic tester before and after treatment; balance training instrument was used to measure the time-consuming (Time) and average trajectory error (ATE) before and after treatment; knee joint pain and quality of life were evaluated with VAS and WOMAC scale before and after treatment. Results: After training, the isokinetic index, dynamic balance index, VAS and WOMAC were significantly improved in the three groups(P<0.05). Pairwise comparison of PT and H/Q after treatment, C=B>A. There were significant differences between groups of AAR and Time (P<0.05), C>B>A. After treatment, the ATE value was pairwise compared, C>B=A. The comparison between groups was statistically significant of VAS score and WOMAC score after treatment (P<0.05), C>B>A. There were no adverse reactions in the three groups during the treatment period. Conclusion: Isokinetic concentric combined eccentric training can reduce the pain of patients with knee osteoarthritis, enhance the muscle strength and coordination of knee flexion and extensor, and improve the function of knee joint. 2021 Vol. 27 (5): 805-810 [Abstract] ( 77 ) HTML (1 KB)  PDF (1268 KB)  ( 178 ) 810 Efficacy of Incision Thread-drawing and Incision Drainage on High Horseshoe-shaped Perianal Abscess and Its Influence on VAS Score and Complications TAN Zhen, QIN Pengpai, TIAN Lei DOI: 10.3969/j.issn.1006-6233.2021.05.023 Objective: To explore the effects of incision thread-drawing and incision drainage on high horseshoe-shaped perianal abscess and its influence on VAS score and complications. Methods: 384 patients with high horseshoe-shaped perianal abscess treated in our hospital from January 2017 to December 2019 were retrospectively analyzed. Propensity score matching was used to successfully match the incision drainage group (control group, 92 cases) and the incision thread-drawing group (observation group, 92 cases) in accordance with the ratio of 1:1. The perioperative parameters, operative efficacy, postoperative recovery status [grading of incision redness and swelling, visual analogue scale (VAS)], incidence rate of complications, postoperative incision infection and recurrence within 3 months were compared between the two groups. Results: The operative time, postoperative recovery time, hospital stay and wound healing time in observation group were shorter than those in control group, and the hospitalization cost was lower than that in control group (P<0.05), and the good improvement rate was higher than that in control group (P<0.05), and the incidence rate of complications was lower than that in control group (P<0.05). At 7d after surgery, the grading of incision redness and swelling in observation group was better than that in control group, and the VAS score was lower than that in control group (P<0.05). The postoperative incision infection rate and recurrence rate within 3 months in observation group were lower than those in control group (P<0.05). Conclusion: Compared with incision drainage for high horseshoe-shaped perianal abscess, incision thread-drawing can effectively improve the operative efficacy, promote postoperative recovery, shorten hospital stay, relieve postoperative pain and incision redness and swelling, reduce occurrence of complications, and lower the postoperative infection and recurrence rate. 2021 Vol. 27 (5): 810-814 [Abstract] ( 125 ) HTML (1 KB)  PDF (1240 KB)  ( 123 ) 814 Curative Effect and Safety of Hip Replacement Through Superpath Approach and Traditional Posterolateral Approach on Femoral Neck Fracture CAO Liangguo DOI: 10.3969/j.issn.1006-6233.2021.05.024 Objective: To analyze the differences in curative effect and safety of hip replacement through superpath approach and traditional posterolateral approach on femoral neck fracture. Methods: A retrospective analysis was performed on the treatment data of 138 patients with femoral neck fracture who underwent hip replacement in the hospital from December 2017 to December 2019. According to different surgical approaches, patients given superpath approach and traditional posterolateral approach were included in study group (n=78) and control group (n=60), respectively. The surgical related conditions, postoperative curative effect, VAS score, postoperative leaving bed time, hospital stay, standing walking test and incidence of complications were compared between the two groups. Results: The surgical related conditions such as operation time, intraoperative blood loss and incision length in study group were significantly better than those in control group (P<0.05). There were significant differences in postoperative good rate between study group and control group (94.87% vs 73.33%) (P<0.05). At 48h after surgery, the postoperative recovery indexes such as drainage volume, leaving bed time, hospital stay, hospitalization cost and standing walking test in study group were better than those in control group (P<0.05). The incidence of postoperative complication in study group was significantly lower than that in control group (7.69% vs 36.67%). Conclusion: The hip replacement through superpath approach for treatment of femoral neck fracture can promote rapid recovery of patients, with significant curative effect and good safety. 2021 Vol. 27 (5): 814-819 [Abstract] ( 86 ) HTML (1 KB)  PDF (1481 KB)  ( 155 ) 819 Curative Effect of Posterior Short-Segment Combined with Vertebral Pedicle Internal Fixation for Thoracolumbar Burst Fracture PANRUKE Wusiman, AIKEPAER Wubuli, YANG Lei, et al DOI: 10.3969/j.issn.1006-6233.2021.05.025 Objective: To analyze the curative effect of posterior short-segment combined with vertebral pedicle internal fixation for thoracolumbar burst fracture. Methods: 176 patients with thoracolumbar burst fractures admitted to the hospital from June 2016 to January 2018 were enrolled. Among them, 92 patients underwent posterior short-segment combined with vertebral pedicle internal fixation (observation group). 84 patients underwent long-segment pedicle fixation through posterior approach (control group). The perioperative indicators, score of visual analogue scale (VAS) of pain in back and loin, Oswestry dysfunction index (ODI), Frankel functional grading at the last follow-up, Cobb's angle and improvement of vertebral anterior edge height were compared between the two groups. The occurrence of adverse events was observed. Results: The operation time, intraoperative blood loss and postoperative hospital stay in observation group were significantly less than those in control group (P<0.05). At the last follow-up, there was no significant difference in the improvement of VAS score, ODI score or Frankel functional grading between the two groups (P>0.05), while the recovery of Cobb's angle and vertebral anterior edge height in observation group was significantly better than that in control group (P<0.05). There were no adverse events after operation in both groups. And there was no case with the failure of internal fixation. Conclusion: Posterior short-segment combined with vertebral pedicle internal fixation can effectively restore the height of the injured vertebrae in the treatment of thoracolumbar burst fracture and correct the kyphosis. The curative effect is better than that of long-segment pedicle fixation. 2021 Vol. 27 (5): 819-823 [Abstract] ( 72 ) HTML (1 KB)  PDF (1408 KB)  ( 116 ) 823 Effect of Hemodialysis Combined with Hemodiafiltration and Hemoperfusion on Renal Anemia in Patients MIAO Changxian, BAO Pin, YAN Xiaolu, et al DOI: 10.3969/j.issn.1006-6233.2021.05.026 Objective: To explore the effect of hemodialysis combined with hemodiafiltration and hemoperfusion on renal anemia in patients. Methods: 100 patients who underwent maintenance hemodialysis in our hospital from August 2018 to April 2020 were selected. According to the simple random number method, they were divided into group A (hemodialysis combined with hemodiafiltration) and group B (hemodialysis combined with hemoperfusion), with 50 cases in each group. The renal anemia indicators [hemoglobin (Hb), hematocrit (Hct), reticulocyte percentage (Ret), serum iron] and blood calcium, blood phosphorus and parathyroid hormone (iPTH) were compared between the two groups before and after treatment, and the incidence rate of adverse events was counted in the two groups. Results: After treatment, Hb, Hct, Ret and serum iron were significantly higher than those in group A (P<0.05). After treatment, the blood calcium level in group B was significantly higher than that in group A while the levels of blood phosphorus and iPTH were significantly lower than those in group A (P<0.05). The incidence rate of adverse reactions in group B was significantly lower than that in group A (P<0.05). Conclusion: Compared with hemodialysis combined with hemodiafiltration can more effectively correct renal anemia, reduce iPTH level and improve calcium and phosphorus metabolism in patients with maintenance hemodialysis. 2021 Vol. 27 (5): 823-826 [Abstract] ( 105 ) HTML (1 KB)  PDF (1225 KB)  ( 233 ) 827 Relationship between Arterial Blood Flow Parameters and Prognosis of Threatened Abortion in Early Pregnancy ZHANG Liying, DING Yuanyuan, SUN Yaqin DOI: 10.3969/j.issn.1006-6233.2021.05.027 Objective: To investigate the predictive value of arterial blood flow parameters on threatened abortion in early pregnancy and its relationship with prognosis. Methods: 200 pregnant women with threatened abortion of 5-8 weeks in our hospital from January 2017 to January 2018 were selected as the study group, and 100 normal early intrauterine pregnancy women were selected as the control group. All patients underwent transabdominal ultrasound, uterine artery blood flow parameters were measured, and all pregnant women were followed up to 16 weeks. In the study group, 102 cases of pregnant women with failure of fetal protection were regarded as failure group, 98 cases of pregnant women with success of fetal protection were regarded as success group. The arterial blood flow parameters of the three groups were compared, and the diagnostic value of the arterial blood flow parameters for pregnancy outcome was compared. The serum progesterone and β -hcg levels of the three groups were compared, and the endometrial thickness and the endometrial vascularization parameters of the study group and the control group were compared. Results: There was no significant difference in the peak systolic flow rate of the three groups of pregnant women (P>0.05); the end-diastolic flow rate of the pregnant women in the failed pregnancy loss group was lower than that in the successful pregnancy loss group and the control group, and the pulsation index and resistance index were higher than those in the successful pregnancy loss group and the control group (P<0.05); the end-diastolic flow rate, pulsatility index, and resistance index of the successful fetal pregnancy group were higher than those of the control group (P<0.05). The cut-off value, sensitivity and specificity of uterine artery blood flow parameters have good diagnostic value. The receiver operating curve shows that the peak systolic flow rate/end-diastolic flow rate (S/D), pulsatility index (PI), and resistance index (RI) all have good diagnostic value. The levels of serum progesterone and β-HCG in the successful and unsuccessful groups were lower than those in the control group, and the levels of serum progesterone and β-HCG in the unsuccessful group were lower than those in the unsuccessful group (P<0.05). There was no significant difference in endometrial thickness between the two groups of pregnant women (P>0.05); the endometrial volume, vascular index (VI), blood flow index (FI), and vascular blood flow index (VFI) of the study group were lower than those of the control group (P<0.05). Conclusion: Arterial blood flow parameters have higher predictive value for threatened abortion in early pregnancy, and their sensitivity and specificity are higher, which is beneficial to maternal prognosis and worthy of further clinical promotion. 2021 Vol. 27 (5): 827-830 [Abstract] ( 139 ) HTML (1 KB)  PDF (1229 KB)  ( 110 ) 831 Effects of Self-made Liji Yangyin Tongluo Decoction Combined with Acupuncture and Moxibustion in the Treatment of Ischemic Stroke and Its Influence on Intracranial Blood Flow and Levels of Peripheral Blood APN ET and Cor ZHANG Ling, HUANG Rong, WEI Congxia, et al DOI: 10.3969/j.issn.1006-6233.2021.05.028 Objective: To explore the effects of self-made Liji Yangyin Tongluo decoction combined with acupuncture and moxibustion in the treatment of ischemic stroke (IS) and its influence on intracranial blood flow and levels of peripheral blood adiponectin (APN), endothelin (ET) and cortisol (Cor). Methods: 186 patients with IS were divided into control group and observation group according to the random number table method, with 93 cases in each group. Control group was treated with conventional western medicine combined with self-made Liji Yangyin Tongluo decoction, and observation group was added with acupuncture and moxibustion based on control group. After 30 d of treatment, the clinical efficacy, neurological function and motor function and quality of life, intracranial blood flow velocity and serum biochemical indicators were observed in the two groups. Results: The total effective rate of treatment was higher in observation group than in control group (90.32% vs 79.57%) (P<0.05). After treatment, the National Institutes of Health Stroke Scale (NIHSS) score in the two groups was significantly reduced while the Fugl-Meyer motor function scale score, modified Barthel index (MBI) score and stroke-specific quality of life scale (SS-QOL) score were significantly increased compared to before treatment, and the blood flow velocities of anterior cerebral artery (ACA), middle cerebral artery (MCA) and posterior cerebral artery (PCA) were obviously increased compared with those before treatment, and the level of APN was significantly lower than that before treatment while the levels of ET and Cor were markedly higher than those before treatment, and the changes in observation group were larger than those in control group (P<0.05). Conclusion: Self-made Liji Yangyin Tongluo decoction combined with acupuncture and moxibustion has significant efficacy in the treatment of IS, and it can significantly improve the neurological function, motor function and intracranial blood flow status. 2021 Vol. 27 (5): 831-835 [Abstract] ( 100 ) HTML (1 KB)  PDF (1243 KB)  ( 170 ) 835 Influence of Operation Combined with Corneal Limbal Stem Cell Autograft Transplantation on Visual Quality Corneal Diopter and Tear Film Function in Pterygium Patients HUANG liumeng, XIE Shunong DOI: 10.3969/j.issn.1006-6233.2021.05.029 Objective: To treat pterygium patients with pterygium excision combined with corneal limbal stem cell autograft transplantation, and to explore the effects on visual quality, corneal diopter and tear film function. Methods: A total of 120 pterygium patients who were treated in the hospital from September 2017 to December 2019 were enrolled as the research objects. They were divided into study group (n=60) and control group (n=60) by random number table method. The control group was treated with pterygium excision, while study group was treated with corneal limbal stem cell autograft transplantation after pterygium excision. Their effects on visual quality, corneal diopter and tear film function were analyzed. Results: After surgery, SR and IMTFcutoff were significantly increased, while TF-OS was decreased in both groups. SR and IMTFcutoff in study group were significantly higher than those in control group, while TF-OS was lower than that in control group (P<0.05). After surgery, UCVA, corneal vertical curvature and horizontal curvature were significantly increased, while CAD was decreased in both groups. UCVA, corneal vertical curvature and horizontal curvature in study group were significantly lower than those in control group, while CAD was higher than that in control group (P<0.05). After surgery, BUT and SIT were significantly increased in both groups, which were significantly higher in study group than control group (P<0.05). After surgery, scores of OSDI and CFS were significantly decreased in both groups, which were lower in study group than control group (P<0.05). After surgery, incidence of complications and recurrence rate in study group were 1.67% and 5.00%, which were significantly lower than those in control group (13.33%, 18.33%) (P<0.05). Conclusion: Pterygium excision combined with corneal limbal stem cell autograft transplantation in the treatment of pterygium can significantly improve corneal diopter, tear film function and visual quality, which can reduce damage to eye epithelial cells and postoperative recurrence rate, with better prognosis. 2021 Vol. 27 (5): 835-840 [Abstract] ( 90 ) HTML (1 KB)  PDF (1264 KB)  ( 190 ) 840 Risk Factors of Upper Gastrointestinal Bleeding in Patients with Coronary Heart Disease After PCI HAO Jinyao, CHENG Dejun, WANG Kecheng DOI: 10.3969/j.issn.1006-6233.2021.05.030 Objective: To investigate the risk factors of upper gastrointestinal bleeding in patients with coronary heart disease after percutaneous coronary intervention (PCI). Methods: The data of 1960 patients with coronary heart disease admitted to our hospital from June 2015 to June 2019 and treated by PCI were retrospectively analyzed. In the follow-up of 1 year after surgery, the incidence of upper gastrointestinal bleeding was counted, patients with upper gastrointestinal bleeding were recorded as the occurrence group, and patients without upper gastrointestinal bleeding were recorded as the non-occurring group, the basic data of the two groups were compared, and the independent risk factors for upper gastrointestinal tract after PCI were determined by Logistic multivariate analysis. Results: In the follow-up of 1 year, a total of 20 cases of 1960 coronary heart disease patients after PCI had upper gastrointestinal bleeding, the incidence was 1.07%. Univariate analysis showed that there were no significant difference in the proportions of gender, smoking history, drinking history, comorbidities, time from onset of coronary heart disease to consultation, medication after PCI, diseased branches, location of the lesion, degree of stenosis , hemoglobin, and platelet count between the two groups (P>0.05) ), there were statistically significant differences in the proportions of age, past history of digestive tract disease, chronic renal insufficiency, history of acute myocardial infarction, PCI operation time and creatinine clearance (P<0.05). Logistic multivariate analysis showed that age > 60 years old, past history of digestive tract disease, chronic renal insufficiency, history of acute myocardial infarction, PCI operation time > 90 min and creatinine clearance ≤ 60 mL/min all affect the upper digestion of patients with coronary heart disease after PCI independent risk factors for tract bleeding. Conclusion: Patients with coronary heart disease after PCI are at risk of upper gastrointestinal bleeding, and older >60 years old, past gastrointestinal medical history, chronic renal insufficiency, history of acute myocardial infarction, PCI operation time> 90 min and creatinine clearance ≤ 60 mL/min are all independent risk factors affecting upper gastrointestinal bleeding in patients with coronary heart disease after PCI. 2021 Vol. 27 (5): 840-845 [Abstract] ( 141 ) HTML (1 KB)  PDF (1255 KB)  ( 173 ) 845 Craniofacial Features of Adolescent Skeletal Type-II High-Angle Disease and the Clinical Effect of Implants Anchorage Combined with MBT Orthodontic Technique WANG Hongyu, DONG Shitao, et al DOI: 10.3969/j.issn.1006-6233.2021.05.031 Objective: To explore the craniofacial features of adolescent skeletal type-II high-angle disease and the clinical effect of implants anchorage combined with MBT orthodontic technique. Methods: A total of 70 adolescents with skeletal type-II high-angle disease who were treated in the hospital from January 2015 to January 2018were enrolled. The adolescents were divided into combination group (30 cases) and control group (30 cases) by random number table method. The combination group was treated with implants anchorage combined with MBT straight wire orthodontics, while control group was treated with MBT straight wire orthodontics combined with non-implant anchorage. The total response rate of surgery, differences in molar displacements, convex distance and inclination angle of maxillary central teeth before and after surgery were compared between the two groups. The occurrence of complications such as root absorption and periodontal lesions during treatment in both groups was statistically analyzed. Results: X-rays head positioning analysis showed that ALFH/PLFH, U1-L1/N-Me, ANS-U1/N-Me and Me-l1/N-Me in vertical direction in high-angle disease group were greater than normal values, soft tissue nasolabial angle (Cm-SN-Ls) amd H2 angle (NB-PgLs) were greater than normal values (P<0.05). The total response rate of surgery in combination group was higher than that in control group, while incidence of complications during treatment was lower than that in control group (P<0.05). After treatment, differences of molar displacements in combination group were less than those in control group, while difference in convex distance and inclination angle of maxillary central teeth were greater than those in control group (P<0.05). Conclusion: Craniofacial features of adolescent skeletal type-II high-angle disease include maxillary protrusion and mandibular retraction. The effective rate of implants anchorage combined with MBT orthodontic technique is high in treatment of adolescent skeletal type-II high-angle disease, which can reduce incidence of postoperative complications. 2021 Vol. 27 (5): 845-850 [Abstract] ( 91 ) HTML (1 KB)  PDF (1624 KB)  ( 304 ) 850 Efficacy of Intermittent Positive Pressure Ventilation Combined with Pulmonary Surfactant in the Treatment of Neonatal Respiratory Distress Syndrome ZHU Daomou, ZHONG Lihua, CHEN Caihua DOI: 10.3969/j.issn.1006-6233.2021.05.032 Objective: To explore the efficacy of intermittent positive pressure ventilation (IPPV) combined with pulmonary surfactant (PS) in the treatment of neonatal respiratory distress syndrome (NRDS). Methods: The data of 153 NRDS patients were analyzed retrospectively from December 2017 to December 2019. All the children were treated with IPPV, and the children with PS were included in the study group (n = 82 cases), while those without PS treatment were included in the control group (n=71). The curative effect, related treatment, arterial blood gas analysis indexes before and after treatment, incidence of adverse reactions, and lung function indexes during follow-up were compared between the two groups. Results: The total response rate in the study group was significantly higher than that in the control group (96.34% vs 84.51%) (P<0.05). The oxygen therapy time, ventilation time, treatment costs and hospital stay of the study group were significantly shorter and lower than those of the control group (P<0.05). After treatment, the arterial partial pressure of carbon dioxide (PaCO2), oxygenation index (OI), arterial partial pressure of oxygen (PaO2), and blood oxygen saturation (SpO2) were significantly improved (P<0.05). And the improvement in the study group was significantly greater than that in the control group (P<0.05). The incidence of bronchopulmonary dysplasia was significantly lower in the study group than in the control group (P<0.05). The study group had lower respiratory rate than the control group, and the tidal volume, volume to peak tidal expiratory flow and time to peak were significantly larger and longer than the control group (P<0.05). Conclusion: Applying iPPV combined with PS in the treatment of NRDS can significantly improve the arterial blood gas analysis indexes in children, and shorten the time of treatment, with significant therapeutic effects. 2021 Vol. 27 (5): 850-855 [Abstract] ( 97 ) HTML (1 KB)  PDF (1408 KB)  ( 128 ) 855 Bedside Ultrasound Evaluation of the Effect of Nalbuphine Combined with Remifentanil on Gastric Emptying Function in Mechanically Ventilated Patients NI Xun, XU Ke, DING Tingting, et al DOI: 10.3969/j.issn.1006-6233.2021.05.033 Objective: To investigate the effect of nalbuphine combined with remifentanil on gastric emptying function in mechanically ventilated patients. Methods: A total of 100 patients requiring mechanical ventilation were selected from this hospital from July to December 2020, and they were randomly divided into remifentanil group and combination group, with 50 cases in each group. Both groups were treated with mechanical ventilation. The remifentanil group was given continuous intravenous pumping of remifentanil for 48 hours for analgesia and sedation, and the combination group was given remifentanil and given nalbuphine continuous intravenous pumping for 48 hours. Ultrasound was used to detect the gastric emptying time (GET) and motility index (MI) before analgesia and 48 hours after analgesia and sedation. Analgesia before and after analgesia and sedation 1 hour, 2 hours, 12 hours, 24 hours, 48 hours,heart rate (HR) and mean arterial pressure (MAP) were recorded. The occurrence of adverse reactions of patients were observed. Results: After analgesia and sedation, GET was prolonged and MI decreased in the two groups, and GET was shortened and MI increased in the combined group (P<0.05). HR slowed and MAP decreased at 1 hour, 2 hours, 12 hours, 24 hours, 48 hours after analgesia and sedation in the two groups (P<0.05). There was no significant difference in HR and MAP at each time point after analgesia and sedation in the two groups (P>0.05). There was no significant difference in the adverse reaction rate between the two groups (P>0.05). Conclusion: Nalbuphine combined with remifentanil is safe and reliable, can shorten the GET of mechanically ventilated patients, and increase the MI without increasing the incidence of adverse reactions. 2021 Vol. 27 (5): 855-859 [Abstract] ( 94 ) HTML (1 KB)  PDF (1253 KB)  ( 280 ) 859 Study on the Effect of Rewarmed and Pressurized Blood Transfusion and Blood Quality MA Yintu, et al DOI: 10.3969/j.issn.1006-6233.2021.05.034 Objective: To study the effect of rapid infusion and observe the change of RBC morphologic,hemolysis rate and blood potassium during the different temperature and compression in order to provide a theoretical basis for the development of wartime rewarmed and pressurized transfusion devices. Methods: The 400ml whole blood stored at 4℃ for 10 days were slected. The pressurizing and rewarming devices were installed on the infusion stand before turning on the power and setting the repressurized parameters to 0Kpa,5Kpa,10Kpa,15Kpa and 20Kpa. The blood transfusion line was inserted into the rewarming tube of the heating instrument, and the thermostat was set up by adjusting the thermostat to set the heating temperature.A simulated rewarmed and pressurized infusion test was performed to detect the red blood cell morphology,the shape of the red blood cells and the blood transfusion effect after the blood flowed through the 40℃、45℃、50℃、55℃ heating tube under different pressure conditions. The variety in free hemoglobin concentration, serum potassium and blood flow rate were compared to evaluate the effect of infusion on blood quality and flow rate under different temperature and pressure conditions compared with untreated. Results: In a certain range for rewarming and pressurizing blood transfusion, the red blood cell hemolysis rate fluctuates at 0.035-0.093%, the measured value of plasma free hemoglobin varied between 0.075-0.184 g/L, and the blood potassium concentration fluctuates between 7.44mmol/L-9.75mmol/L. The test results showed no significant difference before and after infusion (P>0.05). With the increase of external temperature and pressure, a small amount of deformed red blood cells and cell debris could be seen under the high magnification when the temperature is higher than 55℃.The blood flow rate was positively correlated with the temperature and pressure intensity. Conclusion: The rewarmed and pressurized blood would not cause significant erythrocyte hemolysis in a short time under the temperature below 55℃ and pressure less than 20Kpa.The blood free hemoglobin and erythrocyte hemolysis rate had no significant change, with almost no effect on blood quality, but higher temperature and pressure were able to cause mild hemolysis of red blood cells and abnormal changes in morphology. The quality of blood was completely up to the standard requirements of the 2012 edition of the Management Regulations of Clinical Blood Transfusion in Medical Institutions. 2021 Vol. 27 (5): 859-863 [Abstract] ( 197 ) HTML (1 KB)  PDF (1534 KB)  ( 690 ) 864 Efficacy of Bortezomib Combined with Cyclophosphamide and Dexamethasone in the Treatment of Multiple Myeloma and Its Influence on Renal Function Indexes and Adverse Reactions WANG Meijia, YANG Lihua, YAN Qingxia DOI: 10.3969/j.issn.1006-6233.2021.05.035 Objective: To investigate the efficacy of bortezomib combined with cyclophosphamide and dexamethasone (VCD regimen) in the treatment of multiple myeloma (MM) and its influence on renal function indexes and adverse reactions. Methods: 112 patients with MM were randomly divided into control group and observation group according to the random number table method, with 56 cases in each group. Control group was given thalidomide, cyclophosphamide and dexamethasone chemotherapy (CTD regimen), and observation group was given bortezomib, cyclophosphamide and dexamethasone chemotherapy (VCD regimen), and they were treated for 10 courses by taking 3 weeks as a course of treatment. The clinical efficacy of the two groups was evaluated and the blood test results and renal function indexes were compared between the two groups before and after treatment, and the adverse reactions were observed in the two groups. Results: The total clinical effective rate in observation group was significantly higher than that in control group (92.86% vs 71.43%) (P<0.05). After 10 courses of treatment, the serum M protein content, bone marrow plasma cell ratio and blood β2 microglobulin (β2-MG) of the two groups were significantly lower than those before treatment (P<0.05) while the levels of serum hemoglobin (Hb) and platelet factor 4 (PF4) were significantly higher than those before treatment (P<0.05), and the changes in observation group were significantly more than those in control group (P<0.05). After 10 courses of treatment, the levels of blood urea nitrogen (BUN), serum creatinine (SCr) and uric acid (UA) in observation group were significantly lower than those before treatment and those in control group (P<0.05). There were no statistically significant differences in the incidence rates of thrombocytopenia, leukocytopenia, neutropenia, fatigue, nausea and abdominal distension and peripheral neuropathy between the two groups (P<0.05). Conclusion: Bortezomib combined with cyclophosphamide and dexamethasone has a significant efficacy in the treatment of MM. It can effectively reduce the serum M protein, bone marrow plasma cell ratio and β2-MG, and promote the increase of serum Hb content and high-level expression of PF4. And it can play a preventive and ameliorating role in early renal function damage, with mild toxic and side effects and better clinical safety. 2021 Vol. 27 (5): 864-869 [Abstract] ( 261 ) HTML (1 KB)  PDF (1270 KB)  ( 1175 ) 869 Effects of Colloidal Bismuth Pectin Combined with Aluminum Phosphate Gel on Serum GAS ET IL-6 and IL-12 in Patients with Chronic Atrophic Gastritis WU Danna, CHEN Chaocong, YU Huajun, et al DOI: 10.3969/j.issn.1006-6233.2021.05.036 Objective: To explore effects of colloidal bismuth pectin (CBP) combined with aluminum phosphate gel (APG) on serum gastrin (GAS), endothelin (ET), interleukin-6 (IL-6) and interleukin-12 (IL-12) in patients with chronic atrophic gastritis (CAG). Methods: 164 CAG admitted to the hospital from February 2017 to February 2020 were enrolled as study objects. They were divided into observation group and control group by simple random method, 41 cases in each group. The control group was treated with amoxicillin and CBP. On this basis, observation group was treated with APG. After treatment, negative conversion ratio of Helicobacter pylori (Hp), pathological scores, levels of serum GAS, ET, IL-6 and IL-12 were compared between the two groups. The curative effect and safety were evaluated. Results: ①The total response rates of treatment and negative conversion ratios of Hp in observation group and control group were (86.75%, 75.31%) and (69.88%, 59.26%), respectively. There was no significant difference between the groups (P>0.05). ②After treatment, pathological scores such as gastric atrophy, dysplasia, intestinal metaplasia, chronic inflammation and activity in both groups were significantly decreased (P<0.05). After treatment, pathological scores such as atrophy, chronic inflammation and activity in observation group were lower than those in control group (P<0.05). ③After treatment, GAS and IL-12 in both groups were increased, while ET and IL-6 were decreased (P<0.05). The increase or decrease amplitude of each index in observation group was significantly higher than that in control group (P<0.05). ④There was no significant difference in incidence of adverse reactions between the two groups (P>0.05). Conclusion: Pectin bismuth combined with Aluminium Phosphate Gel can improve the inflammatory state and gastric mucosal function in patients with chronic atrophic gastritis. 2021 Vol. 27 (5): 869-873 [Abstract] ( 126 ) HTML (1 KB)  PDF (1245 KB)  ( 174 ) 873 Efficacy of Tolvastan Combined with Nesiritide in the Treatment of Acute Heart Failure and Its Effect on The Levels of NT-proBNP cTnT and sST2 SUN Wei, JIAN Zhifei, et al DOI: 10.3969/j.issn.1006-6233.2021.05.037 Objective: To investigate the efficacy of topaplatin combined with nesiritide in the treatment of acute heart failure (AHF) and its effect on the levels of N-terminal pro-brain natriuretic peptide (NT-proBNP), troponin T (cTnT) and soluble ST2 (sST2). Methods: 200 patients with AHF were randomly divided into the control group (n = 100, nesiritide) and the observation group (n = 100, tovapritide + nesiritide) according to the method of residues of random number table. The clinical efficacy, ventricular remodeling, serum NT-proBNP, cTnT, sST2 expression and safety were compared between the two groups. Results: The total effective rate of the observation group was 93.00%, which was higher than that of the control group (82.00%) (P<0.05). The changes of left ventricular end-systolic volume index (LVESVI), left ventricular end-diastolic volume index (LVEDVI) and left ventricular mass index (LVMI) in the observation group were significantly higher than those in the control group before and after treatment. The changes of serum NT-proBNP, cTnT and sST2 levels in the observation group before and after treatment were significantly higher than those in the control group (P<0.05). The incidence of adverse reactions in the observation group was 6.00%, which was lower than that in the control group (15.00%) (P<0.05). Conclusion: Tovaptan combined with nesiritide is superior to nesiritide in the treatment of AHF. It can not only delay ventricular remodeling, but also reduce the expression of serum NT-proBNP, cTnT and sST2 and reduce the incidence of adverse reactions. 2021 Vol. 27 (5): 873-877 [Abstract] ( 137 ) HTML (1 KB)  PDF (1255 KB)  ( 244 )

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