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2021 Vol. 27, No. 2 Published: 2021-02-28 177 Effect of Serum Containing Yuzhi Zhixue Granule on PTEN/PI3K/AKT Signal Pathway in Ectopic Endometrial Stromal Cells LIU Yanjie, et al DOI: 10.3969/j.issn.1006-6233.2021.02.001 Objective: To investigate the effect of serum containing Yuzhi Zhixue granule on the signal pathway of phosphatase and tensin homolog deleted on chromosome ten (PTEN)/phosphatidyl inositol-3-kinase (PI3K)/protein kinase B (AKT) in ectopic endometrial stromal cells (ESCs). Methods: The serum containing Yuzhi Zhixue granule was prepared, and human ectopic ESCs cells were cultured in vitro, which were divided into control group (blank serum), low dose group (low dose Yuzhi Zhixue granules serum), middle dose group (middle dose Yuzhi Zhixue granules serum) and high dose group (high dose Yuzhi Zhixue granules serum). CCK-8 method was used to detect cell proliferation; cell invasion was detected by Transwell method; the expression of PTEN mRNA was detected by real-time fluorescent quantitative PCR (qRT-PCR); and the expressions of PTEN, p-PI3K, PI3K, p-AKT and AKT proteins were detected by Western blot (WB). Results: Compared with those in the control group, the OD value, invasive cell number, p-PI3K/PI3K, p-AKT/AKT proteins expression levels of ectopic ESCs cells in the low, middle and high dose groups were significantly lower (P<0.05), and the expressions of PTEN mRNA and protein were significantly higher (P<0.05); at the same time, with the increase of dosage of serum containing Yuzhi Zhixue granule, the OD value, invasive cell number, p-PI3K/PI3K, p-AKT/AKT protein expression level of ectopic ESCs cells decreased gradually (P<0.05), and the expressions of PTEN mRNA and protein increased gradually (P<0.05). Conclusion: Serum containing Yuzhi Zhixue granule can inhibit the proliferation and invasion of ectopic ESCs cells, up-regulate the PTEN expression and inhibit PI3K/AKT signaling pathway. 2021 Vol. 27 (2): 177-181 [Abstract] ( 181 ) HTML (1 KB)  PDF (1454 KB)  ( 179 ) 181 Analysis of Uterine Artery Hemodynamic and Thromboelastography Parameters in Patients with Repeated Implantation Failure ZHANG Lirong, ZHU Li, XU Anli, et al DOI: 10.3969/j.issn.1006-6233.2021.02.002 Objective: To explore the relationship between uterine artery hemodynamic parameters and coagulation function in patients with repeated implantation failure. Methods: The patients undergoing in vitro fertilization-embryo transfor (IVF-ET) treatment in our hospital were divided into the first pregnancy success group, the first pregnancy failure group and the repeated implantation failure group. The basic data, oocyte acquisition, transplantation, uterine artery hemodynamics and thromboelastogram parameters were collected and analyzed. Results: There were no statistically significant difference in the basic data, oocyte acquisition and transplantation of the three groups; the uterine artery hemodynamic parameters PI, RI and S/D in the repeated failure group were higher than those in the other two groups, and the difference were statistically significant; The MA value of the repeated failure group were significantly higher than those in the other two groups. Conclusion: It is very necessary to detect the uterine artery hemodynamic parameters and TEG in patients who were suspected with repeated implantation failure. Once the prethrombotic state or the increase of uterine artery blood flow resistance was found, corresponding treatment should be given in time to improve the success rate. 2021 Vol. 27 (2): 181-186 [Abstract] ( 185 ) HTML (1 KB)  PDF (1323 KB)  ( 336 ) 186 Effects of Vardenafil on the Hearing Function and Cochlea Morphology of Guinea Pigs with Drug Induced Hearing Loss LIANG Yuan, HUANG Yueyan, GUO Xia, et al DOI: 10.3969/j.issn.1006-6233.2021.02.003 Objective: To study the effects of vardenafil on the hearing function and cochlea morphology of guinea pigs with drug induced deafness. Methods: Guinea pigs were randomly divided into blank control group, model control group and treatment group, 10 in each group. Two weeks after gentamicin 120mg·kg-1·d-1 injected into Guinea pigs of treatment group and model control group,vardenafil (1mg·kg-1·d-1) and NS(4ml·kg-1·d-1) were injected into Guinea pigs of the above two groups respectively. ABR thresholds were measured respectively prior to the experiment, 1,2 and 4 weeks post-drugs. The morphological changes of cochlea hair cells were observed by scanning electron microscope. Results: After 2 weeks of gentamicin administration, the ABR threshold of treatment group and model control group were significantly higher than that of the blank control group, with statistically significant differences (all P values <0.01). After vardenafil injection, the ABR threshold shift in the treatment group was smaller than that in the model control group, and the difference was statistically significant (all P<0.01). SEM showed that hair cells of the inner and outer hair cells in model control group displayed mess, fusion and imperfections. In the treatment group, the hair cells displayed slight pathological changes, there wasn't significant difference compared with blank control group. Conclusion: Vardenafil can effectively reduce the damage degree of guinea pig cochlea hair cells caused by gentamicin, which shows that it has obvious improvement effect on drug-induced hearing impairment. 2021 Vol. 27 (2): 186-190 [Abstract] ( 129 ) HTML (1 KB)  PDF (2012 KB)  ( 268 ) 190 Effect of LPA on Biological Characteristics of Cervical Cancer Stem Cells and Regulation of RhoA/Rock2 Pathway ZHOU Yong, LUO Baoping, et al DOI: 10.3969/j.issn.1006-6233.2021.02.004 Objective: To study and explore the effects of lysophosphatidic acid (LPA) on the biological characteristics of cervical cancer stem cells and the regulation of RhoA/Rock2 pathway, further clarify the pathogenesis of cervical cancer and develop cervical cancer targets for the future. Point drugs provide theoretical basis. Methods: Siha cell lines were used as primary cells. The cervical cancer stem cells were cultured with balloon-encapsulated cells to observe the ability of cell colony formation. Cell surface markers were detected by FACS. After the successful cultivation of cervical cancer stem cells, they were divided into control group, LPA group and inhibition group. The control group was cultured normally. In the LPA group, LPA of different concentrations (1, 5, 10, 15, 20 umol/L) was added, and the inhibitor group was The optimal proliferative concentration of LPA cells group was based on the addition of Rho kinase inhibitor Y-27632. Cell proliferation was observed and cell growth curves were plotted. The mRNA levels of LPA, RhoA and Rock2 in each group were detected by PCR. The expression of RhoA and Rock2 was detected. Results: After culture, there were suspension cell spheres with CD44+, CK17+, CD34- and CD105- phenotypes. Cervical cancer stem cells were successfully obtained. After adding LPA, the proliferation of cancer cells was significantly promoted, and there was a time In a dose-dependent manner (P<0.05), addition of Y-27632 significantly inhibited cell proliferation (P<0.05). LPA, RhoA, and Rock2 mRNA levels and western blot were significantly higher in LPA group than in the control group and inhibition. Group (P<0.05). Conclusion: LPA can promote the proliferation of cervical cancer stem cells, and its mechanism of action can be achieved by regulating the RhoA/Rock2 pathway. 2021 Vol. 27 (2): 190-194 [Abstract] ( 135 ) HTML (1 KB)  PDF (2419 KB)  ( 305 ) 195 Experimental Study of lncRNA TINCR Targeting MiR-221 Inhibiting Cardiomyocyte Apoptosis in Rats LI Yumi, ZHANG Pei, TANG Yong, et al DOI: 10.3969/j.issn.1006-6233.2021.02.005 Objective: To investigate the effect of long-chain non-coding RNA (lncRNA) tissue differentiation-induced non-protein coding (TINCR) on the apoptosis of rat cardiomyocytes by targeting microRNA (miR)-221 expression. Methods: The cultured rat cardiomyocytes were divided into control group (normal culture), pcDNA3.1 group (transfected pcDNA3.1 empty vector) and TINCR group (transfected pcDNA3.1-TINCR overexpression vector). The expressions of TINCR and miR-221 were detected by real-time fluorescent quantitative polymerase chain reaction(PCR), the target binding relationship between TINCR and miR-221 was detected by double luciferase reporter gene assay, and the cell viability was detected by methylthiazolyldiphenyl-tetrazolium bromide (MTT), the apoptosis was detected by flow cytometry, and the expressions of B-cell lymphoma-2(Bcl-2) and Bcl-2 Associated X Protein(Bax) protein in cells were detected by Western blotting. Transfect miR-221 mimics or inhibitors into cardiomyocytes and analyze the changes of cardiomyocyte apoptosis; miR-221 mimics and pcDNA3.1-TINCR overexpression plasmid were co-transfected into cardiomyocytes to observe the effect of miR-221 on the viability and apoptosis of TINCR overexpression cardiomyocytes. Results: Compared with the control group, the expression level of miR-221, apoptosis rate and the expression level of Bax protein in TINCR group were significantly decreased, while cell survival rate and the expression level of Bcl-2 protein were significantly increased (P<0.05), but there was no statistical significance between pcDNA3.1 group and the control group (P>0.05). The results of double luciferase reporter gene showed that TINCR could bind to miR-221. Compared with the miR-NC group, the apoptosis rate and Bax protein expression level of the miR-221 group were significantly increased, and the Bcl-2 protein expression level was significantly reduced (P<0.05); and the experimental results of anti-miR-221 group are opposite. After up-regulation of miR-221 expression, the effect of TINCR overexpression on myocardial cell viability and apoptosis was significantly reversed (P<0.05).Conclusion: IncRNA TINCR can inhibit cardiomyocyte apoptosis by targeting miR-221 expression. 2021 Vol. 27 (2): 195-201 [Abstract] ( 111 ) HTML (1 KB)  PDF (1753 KB)  ( 350 ) 201 The Correlation Analysis of Asymmetric Dimethylarginine (ADMA) Level and Phenylketonuria Control ZUO Xuemei, et al DOI: 10.3969/j.issn.1006-6233.2021.02.006 Objective: To observe the correlation between the improvement of clinical symptoms and analyze the factors such as symmetric dimethylarginine and arginine by detecting the levels of asymmetric dimethylarginine and phenylalanine in the blood of children with phenylketonuria and to analyze the predictive value of the disease control in patients with phenylketonuria. Methods: Ninety-six children with PKU who were regularly reexamined in Chengde Maternal and Child Health Hospital from September 2016 to March 2019 were selected. The concentration of phenylalanine (Phe) in the blood was detected by high performance liquid chromatography. Patients were divided into a well-controlled group (Phe240~600μmol/L) and a poorly-controlled group (Phe>600μmol/L) considering the control level of phenylalanine; another 100 healthy children were selected and made the control group. The clinical data and laboratory indicators of the three groups of children were compared using high-performance liquid chromatography. The detection covers PHE, symmetric dimethylarginine(SDMA), asymmetric dimethylarginine (ADMA), arginine (ARG), total homocysteine (HCY). Automatic biochemical detector was used to detect serum creatinine (Serum creatinine, SCR) fine Amino acid (Arginine, ARG), triglyceride (TG), total cholesterol (Totalcholesterol, TC), etc., and the clinical symptoms and intelligence scores of children were recorded; the correlation between ADMA and Phe, intelligence scores and other indicators was analyzed; multifactorial logistic regression analysis of independent related factors affecting children's intelligence development was conducted. Results:Compared with the control group, the intelligence score, ADMA, SDMA, Arg, Hcy water of PKU children were significantly lower than the control group, with statistical differences (P<0.05); compared with the poorly controlled group, the ADMA, SDMA, ARG levels and intelligence scores of the well-controlled group were significantly increased, with statistical differences (P<0.05); DMA, SDMA, ARG and HCY were the main factors affecting the concentration of phenylalanine (P<0.05). Among them, ADMA, SDMA, and ARG were negatively correlated with phenylalanine concentration, and positively correlated with intelligence scores. The correlation coefficients were 0.6, 0.7, and 0.4, respectively. Multivariate logistic regression analysis shows that high PHE level (OR=1.952) is an independent related factor that affects intellectual development (P<0.05), while older age (OR=1.074) and high ADMA level (OR=1.556) are protective factors for children's intellectual development (P<0.05). Conclusion: ADMA can be used as an emerging indicator for evaluating dietary metabolism control in children with PKU, and can effectively predict children's intellectual development. 2021 Vol. 27 (2): 201-206 [Abstract] ( 95 ) HTML (1 KB)  PDF (1228 KB)  ( 196 ) 207 Expression of ATF2 in Cervical Cancer Tissues and the Effect of Silencing ATF2 to Inhibit Autophagy on Chemotherapy Sensitivity of Cervical Cancer Cells ZHOU Zhan, LI Yi DOI: 10.3969/j.issn.1006-6233.2021.02.007 Objective: To investigate the expression of ATF2 in cervical cancer tissues and the effect of silencing ATF2 on the sensitivity of cervical cancer cells to the inhibition of autophagy. Methods: Immunohistochemical method was used to detect the expression of ATF2 in cervical cancer tissues; qRT-PCR and Western blot were used to detect the expression of ATF2 in cervical cancer tissues and cervical cancer cell lines; Cell clone formation experiment was used to detect the proliferation ability of HeLa cells; Western blot was used to analyze of the ratio of LC3 II/LC3 I protein expression levels in HeLa cells; Cellular immunofluorescence staining was used to detect the number of autophagosomes in HeLa cells; Cell flow cytometry was used to detect the apoptosis rate of HeLa cells; Western blot was used to detecte the expression of Bax and Bcl-2 protein in HeLa cells. Results: ATF2 was highly expressed in cervical cancer tissues and cervical cancer cell lines (P<0.01); Silent ATF2 combined with DDP reduced the proliferation capacity of HeLa cells (P<0.001), the ratio of LC3 II/LC3 I protein expression level (P<0.01) and the number of autophagosomes (P<0.01); Silent ATF2 combined with DDP increased the apoptosis rate of HeLa cells (P<0.01), Bax protein expression level was up-regulated (P<0.001), Bcl-2 protein expression level was down-regulated (P<0.001). Conclusion: Atf2 is highly expressed in cervical cancer tissues and cervical cancer cells. Silencing Atf2 inhibits autophagy, enhances the sensitivity of HeLa to DDP, and increases the apoptosis rate, thus enhancing the chemosensitivity of HeLa cells. 2021 Vol. 27 (2): 207-211 [Abstract] ( 120 ) HTML (1 KB)  PDF (2059 KB)  ( 276 ) 211 Experimental Study on the Effect of Metformin on Autophagy in Reducing the Damage of Trophoblastic Cells Induced by High Glucose XIANG Meng, et al DOI: 10.3969/j.issn.1006-6233.2021.02.008 Objective: To investigate the effect of metformin on the trophoblastic cells injury induced by high glucose and its mechanism. Methods: Human chorionic trophoblastic HTR8-SVneo cells were cultured in vitro to establish a high glucose injury model, which was randomly divided into control group, model group, metformin I group, metformin II group and metformin III group. MTT method was used to detect the change of cell survival rate; Apoptosis was detected by Annexin V-FITC/PI method; the expressions of autophagy related gene 7 (ATG7) and Beclin-1 mRNA were detected by real-time fluorescence quantitative PCR (RT-qPCR); and the expressions of ATG7, Beclin-1, microtubule associated protein light chain 3 I/II (LC3I/II) and nuclear porin 62 (p62) were detected by Western blot. Results: The optimal concentration of high glucose induced model was 30 mmol/L. Compared with those in the control group, the HTR8-SVneo cell survival rate and p62 protein expression level in the model group were significantly lower (P<0.05), while the apoptosis rate, LC3II/LC3I value, Beclin-1, ATG7 mRNA and protein expressions were significantly higher (P<0.05), in addition, compared with those in the model group, HTR8-SVneo cell survival rate and p62 protein expression level in metformin I group, metformin II group and metformin III group increased in turn (P<0.05), while the apoptosis rate, LC3II/LC3I value, Beclin-1, ATG7 mRNA and protein expressions decreased in turn (P<0.05), in a dose-dependent manner. Conclusion: Metformin may reduce the damage of human chorionic trophoblastic cells by inhibiting autophagy induced by high glucose. 2021 Vol. 27 (2): 211-217 [Abstract] ( 127 ) HTML (1 KB)  PDF (1657 KB)  ( 318 ) 217 Interventional Effect of Edaravone Combined with 20% Human Albumin on NIHSS Scores and Serum cFN SAA Expression in Patients with Severe Cerebral Hemorrhage WANG Luna, QIAN Jian, YANG Guangyu, et al DOI: 10.3969/j.issn.1006-6233.2021.02.009 Objective: To investigate the effect of edaravone combined with 20% human albumin on NIHSS scores and serum cell fibronectin (cFN) and amyloid A (SAA) expression in patients with severe cerebral hemorrhage Methods: 126 patients with severe cerebral hemorrhage in our hospital from August 2017 to October 2019 were analyzed and divided into observation group (n = 42), control group A (n = 42) and control group B (n = 42). On the basis of conventional treatment, control group A was treated with edaravone, control group B was treated with 20% human albumin, and observation group was treated with edaravone combined with 20% human albumin, All were treated for ten days. The efficacy, adverse reactions, and intracranial pressure, hematoma volume, NIHSS score, Glasgow Coma Scale score (GCS), Barthel Index score (BI), serum cFN, and SAA levels were compared between the three groups before and after treatment. Results: The total effective rate in the observation group after treatment for ten days was 95.24%, which was higher than that of the control group A 69.05% and the control group B 80.95% (P<0.05). The intracranial pressure and hematoma volume of the three groups were reduced after treatment for ten days, and the observation group was lower than those in the control A and B groups (P<0.05). After ten days of treatment in the three groups, the NIHSS scores were lower than before treatment, and the observation group was lower than those in control group A and control B. The GCS and BI scores were higher than before treatment, and the observation group was higher than those in control group A and control group B (P<0.05). The serum cFN and SAA levels in the three groups were lower than those before treatment, and the observation group was lower than those in the control A and B groups (P<0.05). The incidences of adverse reactions in observation group, control group A and control group B were 16.67%, 9.53%, and 7.14%, and there was no significant difference between the groups (P>0.05). Conclusion: Edaravone combined with 20% of human albumin in the treatment of patients with severe cerebral hemorrhage can promote hematoma absorption, improve consciousness and nerve function, improve daily living ability, reduce serum cFN and SAA levels have significant efficacy and high safety. 2021 Vol. 27 (2): 217-222 [Abstract] ( 128 ) HTML (1 KB)  PDF (1222 KB)  ( 270 ) 222 Value of HPV Genotyping Combined with TCT in Screening of Cervical Lesions YAO Xiaoyan, ZHOU Fangfang, MA Weiwei DOI: 10.3969/j.issn.1006-6233.2021.02.010 Objective: To explore the value of human papillomavirus (HPV) genotyping and Thin-Cytologic Test (TCT) in screening cervical lesions. Methods: A total of 984 females with regular sexual life who were treated and completed HPV DNA and TCT screening at the outpatient clinic of the hospital between January and October 2019 were selected as the research subjects. Females with HPV positive and TCT ≥ atypical squamous cells with unclear significance (ASC- US) or HPV negative but TCT ≥ low degree squamous intraepithelial lesion (LSIL) underwent colposcopy biopsy. Pathology was taken as the golden standard to evaluate the value of HPV DNA, TCT and their combined screening in diagnosis of cervical lesions. Results: A total of 252 cases of HPV infections were detected in 984 subjects, with an infection rate of 25.61%. The top five HPV infection subtypes were HPV 16 (35.32%), HPV 52 (25.79%), HPV 58 (19.44%) , HPV 18 (9.52%) and HPV 11 (7.94%). Among them, there were 208 cases (82.54%) with single infection and 44 cases (17.46%) with multiple infections. 78 cases were positive for TCT, with a positive rate of 7.93%. Colposcopy and pathological biopsy confirmed 53 cases (67.95%) of abnormalities, including 33 cases of CIN grade 1, 8 cases of CIN grade 2, 7 cases of CIN grade 3, and 5 cases of cervical cancer. The coincidence rate of TCT screening results and pathological examination (52.83%) was lower than HPV (81.13%) and TCT combined with HPV screening (86.79%) (P<0.05). There was no statistically significant difference in the coincidence rate with pathology between TCT combined with HPV screening and single HPV screening (P>0.05). Conclusion: Compared with pathological results, the overall coincidence rate of HPV DNA combined with TCT screening for precancerous cervical lesions and cervical cancer is higher than that of TCT or HPV, which can make up for the defects of TCT screening alone. However, advantages of combined screening with TCT are not significant, compared with HPV screening alone. 2021 Vol. 27 (2): 222-227 [Abstract] ( 133 ) HTML (1 KB)  PDF (1234 KB)  ( 228 ) 228 Expression and Significance of Cx43-Mediated GJIC and mTOR in Gastric Cancer LIU Huan, ZHAO Yang, SHEN Xingbin, et al DOI: 10.3969/j.issn.1006-6233.2021.02.011 Objective: To discuss the expressions and significance of gap junction intercellular communication (GJIC) mediated by connexin43 (Cx43) and mammalian target of rapamycin (mTOR) in gastric cancer tissues. Methods: The expressions of Cx43 and mTOR in gastric tumor lesions and normal gastric mucosal tissues of 60 patients with gastric cancer were detected by immunohistochemical (SP) and qRT-PCR, and their relationships with clinicopathological parameters was analyzed. Results: The expressions of Cx43 mRNA and protein in gastric tumor were lower than those in normal gastric mucosa.However, the expressions of mTOR mRNA and protein were higher than those of normal gastric mucosa (All P<0.01). Regardless of mRNA expression level or protein expression level, Cx43 was associated with the differentiation degree, TNM stage, invasion depth and lymphatic metastasis of gastric cancer (all P<0.05). However, mTOR mRNA and protein were correlated with the differentiation degree, TNM stage and invasion depth of gastric cancer (all P<0.05). There was no significant difference between protein expression level and lymphatic metastasis (P=0.10), but mRNA expression level was correlated with lymphatic metastasis (P<0.05). Correlation analysis showed that the expression of Cx43 protein was negatively correlated with the expression of mTOR protein in gastric cancer patients (r=-0.656, P<0.01), and the expression of Cx43 mRNA was negatively correlated with the expression of mTOR mRNA(r=-0.528, P< 0.01). Conclusion: Cx43 and mTOR are involved in the occurrence and development of gastric cancer, related to the invasion and metastasis of gastric cancer, and in the evolution of gastric cancer, the decreased expression of Cx43-mediated GJIC may stimulate the increased expression of mTOR , and Cx43 may be a potential therapeutic target for gastric cancer. 2021 Vol. 27 (2): 228-233 [Abstract] ( 186 ) HTML (1 KB)  PDF (2023 KB)  ( 252 ) 233 Study on the Correlation Between PD-L1 at 22C3 Locus and Ki67 EGFR and Blood Indicators MAO Xuelian, WANG Chengxia, CHEN Yan, et al DOI: 10.3969/j.issn.1006-6233.2021.02.012 Objective: To explore the relationship between the expression of programmed death receptor ligand-1(PD-L1) and the mutations of KI67 and EGFR in non-small-cell lung cancer. Methods: The expression of PD-L1(22C3)and Ki67 in 121 NSCLC tissues from November 2018 to July 2019 was detected with immunohistochemistry. The ARMS method was used to detect the mutations of the corresponding tissue EGFR gene, and the flow cytometry method was used to detect the proportion of serum CD3+, CD4+ and CD8+ T cells. The biochemical luminescence method was used to detect LDH, CEA, CA199 and CY211. The χ2 analysis was used to analyze the differences in the distribution of PD-L1 expression in clinical factors, and the Spearman test was used to analyze the correlation between PD-L1 expression and EGFR gene, ki67 and blood indicators. Results: The low expression rate of PD-L1 was 38% (46/121), the high expression rate was 23.2% (28/121), the high expression rate of ki67 was 56.4% (57/101), and the EGFR mutation rate was 41.7% (20/48). The positive expression rate of PD-L1 in patients with stage III+IV and local lymph node metastasis was significantly higher than that in patients with I+II and without lymph node metastasis, and the difference was statistically significant (χ2=6.636, 7.726, P<0.05). The positive expression rate of PD-L1 was significantly correlated with EGFR mutants, high ki67 expression, increased number of CD8+ cells, and increased LDH, and was positively correlated (P<0.05). Conclusion: The expression rate of PD-L1 (22C3) is high in patients with lung cancer, and the expression rate of PD-L1 is significantly increased in patients with advanced staging, lymph node metastasis, high expression of ki67, EGFR mutation, CD8+ T cell number, and LDH. Related to clinical indicators with poor prognosis, it is an indicator for screening Pembrolizumab treatment and may also become a potential prognostic marker for NSCLC. 2021 Vol. 27 (2): 233-237 [Abstract] ( 185 ) HTML (1 KB)  PDF (1217 KB)  ( 562 ) 238 Expression and Clinical Significance of L1CAM Mel-18 and c-myc in Endometrial Carcinoma ZHANG Jin, et al DOI: 10.3969/j.issn.1006-6233.2021.02.013 Objective: To investigate the expression and clinical significance of L1 cell adhesion molecule (L1CAM), melanoma nucleoprotein-18 (Mel-18) and proto oncogene (c-myc) in endometrial carcinoma. Methods: Endometrial carcinoma and paracancer tissue of 80 patients with endometrial cancer in Liaoning Provincial People's hospital from February 2015 to December 2019 were collected for clinical study. The expressions of L1CAM, Mel-18 and c-myc were detected by immunohistochemistry. The relationship between the expression of L1CAM, Mel-18, c-myc and clinicopathological parameters was analyzed. Pearson method was used to analyze the expression correlation of L1CAM, Mel-18 and c-myc. Results: The positive rates of L1CAM, Mel-18 and c-myc in endometrial carcinoma tissues were significantly higher than those in adjacent normal tissues (P<0.005).The expression of L1CAM, Mel-18 and c-myc protein in endometrial carcinoma tissues was correlated with lymph node metastasis and FIGO stage (P<0.05).L1CAM, Mel-18 and c-myc were positively correlated with each other (P<0.05). Conclusion: The positive rates of L1CAM, Mel-18 and c-myc in endometrial carcinoma are increased, and the expression of the three is positively correlated with each other. The expressions of L1CAM, Mel-18 and c-myc were correlated with lymph node metastasis and FIGO stage of endometrial carcinoma. 2021 Vol. 27 (2): 238-241 [Abstract] ( 139 ) HTML (1 KB)  PDF (1690 KB)  ( 631 ) 242 Effect of Total Thoracoscopic Anatomic Segmentectomy for Small Pulmonary Nodules on Surgical Parameters Pulmonary Function and Drainage Volume XU Shijie, HONG Weidong, JIANG Haifeng, et al DOI: 10.3969/j.issn.1006-6233.2021.02.014 Objective: To investigate the effects of total thoracoscopic anatomic pulmonary segmentectomy on surgical parameters, lung function and drainage volume of pulmonary nodules. Methods: 84 patients with small pulmonary nodules in our hospital from February 2016 to April 2020 were prospectively selected and randomly divided into two groups according to the random number table method. Among them, the control group (42 cases) underwent total thoracoscopic lobectomy, and the study group (42 cases) underwent thoracoscopic segmentectomy. Differences in surgical indicators, lung function indicators (FEV1, FVC, FEV1/FVC) and postoperative complications were compared between the two groups. Results: There was no significant difference in intraoperative lymph node dissection between the two groups (P>0.05), but the intraoperative blood loss, drainage volume, drainage time and hospital stay in the study group were all lower than those in the control group, and the difference was statistically significant (P<0.05).Analysis of variance of repeated measures showed that before treatment, there was no significant difference in FEV1%, FVC% and MMV% between the two groups (P<0.05). After 3, 6 and 12 months of treatment, FEV1%, FVC% and MMV% in the study group were significantly higher than those in the control group (P<0.05).In addition, the total incidence of postoperative complications was 4.76% in the study group and 9.52% in the control group, with no statistically significant difference between the groups (χ2=0.718, P>0.05) . Conclusion: The overall efficacy of total thoracoscopic anatomic pulmonary segmentectomy in the treatment of pulmonary nodules is better than that of lobectomy, which can largely retain the normal lung tissue of the patients and has less impact on the pulmonary function of the patients. 2021 Vol. 27 (2): 242-246 [Abstract] ( 156 ) HTML (1 KB)  PDF (1434 KB)  ( 272 ) 246 Expression and Clinical Significance of Serum Vasohibin-1 in Type 2 Diabetic Nephropathy PEI Shuang, SU Jianrong DOI: 10.3969/j.issn.1006-6233.2021.02.015 Objective: To investigate the expression and clinical significance of serum vasohibin-1(VASH-1) in patients with type 2 diabetic nephropathy (DN). Methods: 252 patients with DN were selected as the research group for prospective study. According to the urinary albumin excretion rate (UAER), they were divided into normal albuminuria group (NA group, n=83), microalbuminuria group (MA group, n=85) and large albuminuria group (LA group, n=84). 85 healthy people in the same period were selected as the control group. 24 hours urine was collected for UAER detection, and venous blood was collected for VASH-1, transforming growth factor-β1(TGF-β1), C-reactive protein (CRP),glycosylated hemoglobin(HbA1c) and erythrocyte sedimentation rate(ESR) detection, which were compared among the groups, and the correlation between VASH-1 and other indexes was analyzed. Results: The levels of VASH-1,TGF-β1,UAER,CRP,HbA1c and ESR in the research group were significantly higher than those in the control group. The comparison between the two groups was statistically significant(P<0.05).The levels of research in NA group, MA group and LA group were as follows: LA group> MA group>NA group; the comparison among the three groups was statistically significant(P<0.05).Pearson correlation analysis showed that VASH-1 was positively correlated with TGF-β1, UAER, CRP, HbA1c and ESR (r=0.526, 0.646, 0.529, 0.273, 0.592, P<0.01). Multiple linear regression analysis showed that TGF-β1, UAER, CRP, HbA1c and ESR were independent risk factors for the change of serum VASH-1 level in DN patients. Conclusion: The increased expression of serum VASH-1 in DN patients is closely related to TGF-β1 and inflammatory markers, which provides a new way to study the mechanism of inflammation and fibrosis, to assist diagnosis, to explore therapeutic targets and to develop new drugs in diabetic renal injury. 2021 Vol. 27 (2): 246-250 [Abstract] ( 108 ) HTML (1 KB)  PDF (1284 KB)  ( 174 ) 250 Changes and Significance of Serum Amyloid A and apoB/ApoA1 Ratio in Diabetic Foot XU Yan, CHEN Jian DOI: 10.3969/j.issn.1006-6233.2021.02.016 Objective: To analyze the changes and significance of serum amyloid A (SAA) and apolipoprotein B (ApoB) to apolipoprotein A1 (ApoA1) (apoB/ApoA1) in diabetic foot (DF). Methods: 262 patients with type 2 diabetes mellitus (DM) were selected as the study object. According to whether DF was complicated, they were divided into DF group (n=128) and DM group (n=134), healthy people without high risk factors of DM such as hyperlipidemia and obesity were selected as the control group (n=135). The changes of SAA and ApoB/ApoA1 in peripheral blood of three groups were compared. To analyze whether SAA, apoB/ApoA1, course of DM, HbA1c, FGB were independent risk factors for DF occurrence, and to explore the correlation between SAA and ApoB/ApoA1 and their predictive value for DF occurrence. Results: The course of DM in DF group was significantly longer than that in DM group (P<0.05), and the levels of ApoB, FBG, HbA1c, SAA and ApoB/ApoA1 in DF group were significantly higher than those in DM group and control group (P<0.05). Logistic multiple linear regression analysis show that course of DM, FBG, HbA1c, SAA and ApoB/ApoA1 were independent risk factors for DF. Pearson correlation analysis showed that SAA was positively correlated with ApoB/ApoA1 (r= 0.486, P<0.001). The prediction sensitivity (85.52%) and specificity (89.30%) of SAA and ApoB/ApoA1 were higher than that of SAA or ApoB / ApoA1 alone (P<0.05). Conclusion: SAA and ApoB/ApoA1 in peripheral blood are independent risk factors for DF, which have certain value for DF prediction. 2021 Vol. 27 (2): 250-254 [Abstract] ( 97 ) HTML (1 KB)  PDF (1398 KB)  ( 358 ) 254 Levels of Serum IL-10, CXCL16 in Patients with Acute Coronary Syndrome and Its Clinical Significance MA Weili, CAI Ying, BAO Fengmei, et al DOI: 10.3969/j.issn.1006-6233.2021.02.017 Objective: To explore the levels of serum interleukin-10 (IL-10) and CXC chemokine ligand 16 (CXCL16) in patients with acute coronary syndrome (ACS) and its clinical significance. Methods: A total of 150 patients with ACS admitted to our hospital from June 2018 to June 2019 were selected as study objects (ACS group), who were divided into acute myocardial infarction group (AMI group, n=79) and unstable angina pectoris group (UAP group, n=71) according to the disease type. Also, the patients were divided into mild lesion group (n=65), moderate lesion group (n=52) and severe group (n=33) according to Gensini score. And the patients were divided into single vessel lesion group (n=60), double vessel lesion group (n=58) and triple vessel lesion group (n=32) according to the number of coronary artery lesions. At the same time, 60 patients with stable angina pectoris (SAP group) and 60 healthy volunteers (control group) were randomly selected as controls. The serum IL-10 and CXCL16 levels were detected by enzyme-linked immunosorbent assay. Results: The levels of IL-10 and CXCL16 in ACS group were higher than those in SAP group and control group (P<0.05). The level of IL-10 and CXCL16 in AMI group were higher than those in UAP group (t=9.186, 6.643; P<0.05). With the severity and number of coronary lesions in ACS group, the IL-10 and CXCL16 increased (P<0.05). The serum IL-10 in ACS group was positively associated with the Gensini scores and the number of coronary lesions (r=0.692, 0.702; P<0.05), and the CXCL16 was positively associated with the Gensini scores and the number of coronary lesions (r=0.637, 0.645; P<0.05). The ROC curve analysis showed that IL-10 and CXCL16 have differential diagnostic value for AMI and UAP, and the combined detection of both IL-10 and CXCL16 have a higher differential diagnostic value. The AUC was 0.860 (95%CI: 0.835~0.907), the sensitivity and specificity were 89.67% and 82.39%, and the accuracy was 86.34%. Conclusion: The up-regulation of serum IL-10 and CXCL16 are involved in the pathogenesis of ACS, which are closely related to the Gensini score of coronary lesions and the number of coronary lesions. Early detection of both IL-10 and CXCL16 can be used as important biochemical indexes for clinical differential diagnosis of ACS and assessment of the severity of coronary lesions. 2021 Vol. 27 (2): 254-259 [Abstract] ( 90 ) HTML (1 KB)  PDF (1267 KB)  ( 149 ) 259 Clinical Study of Autologous Adipose Derived Mesenchymal Stem Cells in the Treatment of Trunk Part Postherpetic Neuralgia ZHANG Hong, ZHANG Shuli, YAN Baili, et al DOI: 10.3969/j.issn.1006-6233.2021.02.018 Objective: To investigate the clinical effect of autologous adipose derived mesenchymal stem cells in the treatment of trunk part (thoracic 3-lumbar 12 segments) postherpetic neuralgia. Methods: 110 patients with trunk part (thoracic 3-lumbar 12 segments) postherpetic neuralgia treated in our hospital from February 2017 to March 2020 were selected as the research objects, and were randomly divided into stem cell group and radiofrequency group (55 cases in each group). The patients in the radiofrequency group were treated with conventional nerve root pulse radiofrequency block, and the stem cell group were treated with autologous adipose derived mesenchymal stem cells. The clinical efficacy, incidence of adverse reactions, satisfaction with pain control, visual analogue scoring(VAS) score, quality of life score and sequelae symptoms (itching, tight binding, ant walking) incidence rate before and after treatment were compared between the two groups. Results: The clinical effect of the stem cell group (92.73%) was higher than that of the radiofrequency group (76.36%)(P<0.05). After 1 and 3 months of treatment, the VAS scores of the two groups were significantly decreased (P<0.05), and the VAS scores in the stem cell group was lower than the radiofrequency group (P<0.05). After 1 and 3 months of treatment, the scores of sleep, diet, emotion, daily life and other quality of life of the two groups were significantly decreased (P<0.05), and the scores in the stem cell group was lower than that of the radiofrequency group (P<0.05). The incidence of adverse reactions in the stem cell group was lower than that in the radiofrequency group (5.45% vs 21.28%, P<0.05), and the pain control satisfaction was higher than that in the radiofrequency group (90.91% vs 70.91%, P<0.05). After treatment, the itching, tight binding and ant sense of the two groups were significantly reduced, and the incidence of itching, tight binding and ant sense in the stem cell group was significantly lower than that in the radio frequency group (P<0.05). Conclusion: Autologous adipose derived mesenchymal stem cells have a good clinical effect in the treatment of patients with trunk part (thoracic 3-lumbar 12 segments) postherpetic neuralgia, which can effectively relieve the degree of pain, improve the quality of life of patients, significantly reduce the incidence of adverse reactions and sequelae symptoms, improve the satisfaction of pain control, so it is worthy of clinical application. 2021 Vol. 27 (2): 259-264 [Abstract] ( 406 ) HTML (1 KB)  PDF (1233 KB)  ( 528 ) 264 Effect of Laparoscopic Oviduct Sparing Surgery on Ovarian Reserve Function and Re-productive Function in Patients with Tubal Pregnancy ZHANG Jin, CHEN Mei, XU Xiulan DOI: 10.3969/j.issn.1006-6233.2021.02.019 Objective: To analyze the effect of laparoscopy on ovarian reserve function and fertility function in tubal pregnancy. Methods: 218 patients with tubal pregnancy in our hospital were retrospectively studied. According to different treatments, they were divided into the conservative group (87 cases), the resection group (89 cases), and the retention group (42 cases). After treatment, sex hormone levels, ovarian function parameters, and the repregnancy status of the 3 groups were compared. Results: There were significant differences in the content of sex hormone (FSH, E2, LH, progesterone) and the level of ovarian function (ovarian sectional area, number of sinusoid follicles, PSV, EDV) among the three groups after treatment (P<0.05). Pair comparison showed that the content of three sex hormones (FSH, E2 and LH) in the retention group was significantly lower than that in the conservative group and the resection group, and the progesterone level and various ovarian function parameters in the retention group were significantly higher than that in the conservative group and the resection group, with statistically significant differences between the two groups (P<0.05). Compared at different time points, the E2, progesterone sex hormone levels and various ovarian function parameters of the three groups showed a significant downward trend with time migration, while the FSH and LH sex hormone levels increased significantly. The incidence of non-pregnancy in the conservative group (39.08%), the excision group (41.57%) and the retention group (23.80%) was statistically significant (P<0.05). The incidence of intrauterine pregnancy in the conservative group (32.18%), the excision group (28.09%), and the retention group (26.19%) had statistically significant differences (P<0.05). Conclusion: Compared with other commonly used therapies, laparoscopic tubal preservation surgery in patients with tubal pregnancy can better preserve fertility and has better ovarian reserve function. 2021 Vol. 27 (2): 264-268 [Abstract] ( 91 ) HTML (1 KB)  PDF (1217 KB)  ( 230 ) 268 Analysis of the Effect of Combined Anterior and Posterior Approach of Knee in the Treatment of Complex Tibial Plateau Fractures ZHAO Bo, ZHENG Zhipan DOI: 10.3969/j.issn.1006-6233.2021.02.020 Objective: To analyze the difference of clinical effect of anterior median approach and combined anterior posterior approach in the treatment of complex tibial plateau fractures. Methods: 114 cases of anterolateral patients with posterolateral tibial plateau fracture admitted in Hanchuan People's Hospital from 2014 to 2017 were selected and randomly divided into the control group (anterolateral medial approach) and the experimental group (anterolateral joint approach), with 57 cases each. The morphological indexes (varus angle, femoral tibial angle, caster angle), range of motion (knee flexion, knee extension), scale scores (Rasmussen score, HSS score) and statistical differences of knee function were compared between the two groups before and after treatment (before operation, after operation, at discharge, 3 months after discharge). The incidence of complications was compared between the two groups. Results: There were significant differences between the two groups in postoperative (caster angle, knee flexion, Rasmussen score), discharge (varus angle, femoral tibial angle, caster angle, knee flexion, knee extension, Rasmussen score, HSS score, knee function) and 3 months after discharge (varus angle, femoral tibial angle, caster angle, knee flexion, knee extension, Rasmussen score, HSS score, knee function) There were significant differences in varus angle, femoral tibial angle and caster angle (P<0.05), knee flexion and extension (P<0.05), Rasmussen score and HSS score (P<0.05), and knee function index (P<0.05). The incidence of complications in the experimental group (28.07%) was lower than that in the control group (57.89%), the difference was statistically significant (P<0.05). Conclusion: The treatment of complex tibial plateau fractures by combined anterior and posterior approach can achieve relatively better clinical efficacy, better recovery of knee joint function and fewer complications. 2021 Vol. 27 (2): 268-275 [Abstract] ( 133 ) HTML (1 KB)  PDF (2913 KB)  ( 187 ) 276 A Research on the Relevance of Residual Cholesterol to Acute Coronary Syndrome GAO Tenglong, QIAN Wenru, ZHAO Chunsheng, et al DOI: 10.3969/j.issn.1006-6233.2021.02.021 Objective: To analyze the changes of residual cholesterol (RC) in patients with acute coronary syndrome (ACS) and study the relevance of RC to ACS. Methods: A total of 300 patients with ACS who were hospitalized in the Department of Cardiology of our hospital from January 2017 to February 2018 were selected as the research objects. According to the diagnostic criteria, ACS patients were sub-grouped: acute ST segment elevation myocardial infarction group (STEMI group), acute non-ST segment elevation myocardial infarction group (NSTEMI group), unstable angina pectoris group (UA group), with 108 cases, 88 cases and 104 cases in each group. 100 patients with chest pain and without ACS were selected and made the non-ACS group. The clinical data of the subjects were collected and the differences of blood lipid levels among the four groups were compared. Results:The levels of total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) and residual cholesterol (RC) in STEMI group, NSTEMI group and UA group were significantly higher than those in non-ACS group (P < 0.05). There was no significant difference in HDL-C and non-HDL-C (P > 0.05). The area under RC curve in ROC was 0.710, which was higher than other blood lipid indexes. Conclusion: RC is associated with acute coronary syndrome, and has a certain predictive value in the diagnosis of acute myocardial infarction. 2021 Vol. 27 (2): 276-278 [Abstract] ( 108 ) HTML (1 KB)  PDF (1287 KB)  ( 393 ) 279 An Analysis of Pathogen Spectrum and Risk Factors for Pulmonary Infection in Kidney Transplant Recipients DENG Cong, SUN Weimin, LIN Meishuang, et al DOI: 10.3969/j.issn.1006-6233.2021.02.022 Objective: To investigate the characteristics, pathogen spectrum and risk factors for pulmonary infection in kidney transplant recipients. Methods: We performed a retrospective cohort study reviewing the medical records of 627 patients who had undergone kidney transplantation at The Second Affiliated Hospital Of Guangzhou Medical University from April 2017 to April 2019. Results: One hundred and five(16.75%)patients developed pulmonary infection during the follow-up period. Five cases(4.76%)of pulmonary infection occurred within 1month after surgery, sixty-one cases(58.10%)occurred within 2～6 months and 39 cases(37.14%)occurred more than 6 months after surgery, respectively. Among 105 patients, 70 patients had been detected positively with pathogens. The pathogens isolated from sputum and blood sampls accounted for 89.33%, isolated from throat swab and pleural effusion sampls accounted for 10.67%, respectively. The pathogens identified in 70 patients indicated that 54 (55.10%) cases were bacteria, 16 (16.33%) were fungus, 12 (12.24%) were cytomegalovirus, 1(1.02%)was pneumocystis carinii and 15 (15.31%) were complicated infection. The most common organisms were Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Stenococcus maltophilia and Staphylococcus aureus. Univariate analysis demonstrated that diabetes history(P=0.044), WBC＜4×109L-1(P=0.015), delayed graft function (P=0.008)and acute rejection(P=0.037)were risk factors of pulmonary infection in kidney transplant recipients. Multivariate logistic regression analysis showed that WBC＜4×109L-1(OR=2.499,95%CI 1.321～4.726, P=0.005), delayed graft function(OR=2.894,95%CI 1.128～7.429, P=0.027) and acute rejection(OR=2.233,95%CI 1.121～4.446, P=0.022)were independent risk factors associated with the development of pulmonary infection in kidney transplant recipients. Conclusion: Pulmonary infection most commonly occurs within 2～6 months after kidney transplantation. Collecting various samples from different sites and combination of different detection methods can increase the detection rate of pathogens. The patients who have WBC＜4×109L-1, delayed graft function or acute rejection after kidney transplantation are at increased risk of developing pulmonary infection. 2021 Vol. 27 (2): 279-283 [Abstract] ( 157 ) HTML (1 KB)  PDF (1451 KB)  ( 304 ) 283 Clinical Value of Spiral CT and PET-CT in the Diagnosis of Lung Cancer and Lymph Node Metastasis WANG Jiafu, LIN Lin, LIN Yaoyun, et al DOI: 10.3969/j.issn.1006-6233.2021.02.023 Objective: To explore the clinical value of spiral CT and PET-CT scan in the diagnosis of lung cancer and lymph node metastasis. Methods: 96 patients with lung cancer and suspected lymph node metastasis admitted to our hospital from January 2018 to August 2019 were selected. Within 2 weeks, spiral CT and PET-CT were performed. Based on the final surgical and pathological results, the efficacy of the two diagnostic methods in the diagnosis of lung cancer and lymph node metastasis was compared. Results: The results of surgical pathology showed that among 96 patients with suspected lung cancer lymph node metastasis, 22 had no lymph node metastasis and 74 had lymph node metastasis. Among them, 32 had local recurrence lesions; a total of 102 metastatic lymph nodes were detected, of which diameter There are 6 metastatic lymph nodes of ≤0.5cm, 75 metastatic lymph nodes of 0.5 ~ 1.0cm, and 21 metastatic lymph nodes of diameter ≥1.0cm. The accuracy, sensitivity, specificity, positive prediction rate, and negative prediction rate of spiral CT and PET-CT in the diagnosis of lung cancer lymph nodes were 75.0%, 74.3%, 77.3%, 91.7%, 47.2%; 94.8%, 97.4%, 86.4% , 96.0%, 90.5%. In the detection of metastatic lymph nodes, the total detection rate of PET-CT was significantly higher than that of spiral CT (P<0.05). In the detection of metastatic lymph nodes, the total detection rate of PET-CT was significantly higher than that of spiral CT (P<0.05). Among them, the detection rate of PET-CT was significantly higher than that of spiral CT on ≤0.5cm and 0.5-1.0cm metastatic lymph node metastasis, and the difference was statistically significant (P<0.05); on ≥1.0cm metastatic lymph node, neither Significant difference, not statistically significant (P>0.05). Conclusion: Compared with spiral CT diagnosis, PET-CT diagnosis of lung cancer and lymph node metastasis diagnostic efficiency is more significant, with clinical application value, but the cost is higher, broad-spectrum is weak, so it can be used as a further diagnostic means to improve the diagnostic accuracy when spiral CT is difficult to determine. 2021 Vol. 27 (2): 283-287 [Abstract] ( 132 ) HTML (1 KB)  PDF (1220 KB)  ( 250 ) 288 Predictive Value of Serum CRP PCT Levels and ISS in Severe Multiple Trauma Patients with Abdominal Injury MA Wujian, LI Yucui DOI: 10.3969/j.issn.1006-6233.2021.02.024 Objective: To analyze the diagnostic value of serum C-reactive protein (CRP), procalcitonin (PCT) and injury severity score (ISS) in patients with severe multiple trauma and abdominal injury, and the prediction of death of patients with organ injury. Methods: The clinical data of 93 patients with severe multiple trauma with abdominal injury in our hospital from August 2017 to January 2020 were retrospectively analyzed. According to the presence or absence of organ injury, the patients were divided into group A (48 cases without organ injury) and group B (45 cases with organ injury). The serum CRP, PCT levels and ISS score were analyzed, and the receiver operating characteristic curve (ROC) was used to analyze the diagnostic value of CRP, PCT and ISS scores in organ injury and to predict death of patients with organ injury. Results: Comparison of PCT, CRP and ISS scores between groups A and B showed statistically significant differences (P<0.05). According to the ROC analysis results, the AUC of PCT, CRP and ISS scores for predicting the death of organ injuries were 0.743, 0.808, and 0.800 respectively; according to the best cut-off value: PCT has a sensitivity of 55.6% and specificity is 97.7%; CRP has a sensitivity of 71.1% and specificity of 83.3%; ISS points have a sensitivity of 60.0% and specificity of 89.6%. In group B, 15 patients with organ injury survived and 30 died. The PCT, CRP and ISS scores of the two types of patients were compared, and the difference was statistically significant (P<0.05). According to the ROC analysis results, the AUC of PCT, CRP and ISS scores for predicting the death of organ injuries were 0.977, 0.976, and 0.960, respectively. According to the best cut-off value: PCT has a sensitivity of 97.0% and a specificity of 86.7%; CRP has a sensitivity of 96.7 and specificity of 86.7%; ISS points have a sensitivity of 98.2% and specificity of 80.0%. Conclusion: If there is organ damage in patients with severe multiple injury and abdominal injury, their PCT, CRP levels and ISS score will be significantly increased, and have certain value in the diagnosis of organ damage, but PCT, CRP levels and ISS have higher value in predicting patients' death. 2021 Vol. 27 (2): 288-292 [Abstract] ( 95 ) HTML (1 KB)  PDF (1512 KB)  ( 218 ) 292 Effects of Acute Normovolemic Hemodilution Combined with Autologous Blood Transfusion on Blood Gas Index Coagulation Function and Transfusion Complications in Patients Undergoing Cardiac Valve Replacement ZHANG Bangjian, LIU Yixin, HAN Qingtao, et al DOI: 10.3969/j.issn.1006-6233.2021.02.025 Objective: To explore the effect of acute isovolumetric hemodilution (ANH) combined with autologous blood transfusion on blood gas indexes, coagulation function and blood transfusion complications in patients undergoing heart valve replacement surgery. Methods: 102 patients who underwent heart valve replacement in Panzhihua Central Hospital from January 2018 to January 2019 were selected as the research objects. The simple random number table method was randomly divided into observation group and control group, with 51 cases in each group. The patients in the control group were transfused with untreated remaining bank blood or machine blood, and the patients in the observation group were transfused with ANH combined with autologous blood. Before blood dilution, 15min, 24h, 48h after the end of the operation, record the blood gas index and coagulation function index at each time period, and record the operation time, intraoperative blood loss, chest drainage volume, fluid replacement volume, allogeneic blood transfusion volume, hospitalization Time and complications of blood transfusion within 1 day after operation. Results: There was no statistically significant difference in the operation time, intraoperative blood loss, fluid supplementation, and hospital stay between the two groups of patients (P>0.05). The observation group had significantly less chest drainage and allogeneic blood transfusion. In the control group, the difference was statistically significant (P<0.05). The PvO2, PvCO2, and Fib levels of the two groups of patients decreased first and then increased, while the levels of PT, APTT, and TT increased first and then decreased. The differences between the time points within the group were statistically significant (P<0.05). Except before hemodilution, the differences between the groups at each time point were statistically significant (P<0.05); there were no statistically significant differences in the pH levels of the two groups at each time point in the group or between the time points. (P>0.05). The incidence of blood transfusion complications in the observation group was lower than that in the control group, but the difference between the groups was not statistically significant (P>0.05). Conclusion:Acute isovolumetric hemodilution combined with autologous blood reinfusion has little effect on blood gas indicators and coagulation function in patients undergoing heart valve replacement surgery. It can significantly reduce the volume of foreign blood transfusion and adverse blood transfusion reactions. The combination of the two can protect blood more effectively. It is a safe and effective way of blood transfusion. 2021 Vol. 27 (2): 292-297 [Abstract] ( 113 ) HTML (1 KB)  PDF (1228 KB)  ( 198 ) 297 The Relationship Between Serum Magnesium Decreased and Coronary Artery Disease Complicated with Diabetes SI Yueqiao, FAN Wenjun, GAO Xiuxin, et al DOI: 10.3969/j.issn.1006-6233.2021.02.026 Objective: To explore the correlation between serum magnesium levels and coronary artery disease (CAD) complicated with diabetes. Methods: A total of 467 eligible inpatients with suspected CAD from January 2015 to June 2017 in our hospital were consecutively enrolled in the study. All of them were divided into CAD diabetes (n=129), CAD non-diabetes (n=201), non-CAD diabetes (n=33), and non-CAD non-diabetic group (n=104). Clinical data was collected and total coronary artery calcification (CAC) score was calculated. Multivariate logistic regression model was used to analyze the risk factors of CAD complicated with diabetes. Results: Dyslipidemia, pulse pressure≥60mmHg, CAC score>400, EF<50% and serum magnesium≤0.77mmol/L are all risk factors for CAD with diabetes, serum magnesium≤0.77mmol/L being the highest risk. The risk of serum magnesium decreased in CAD with diabetes group was 2.806 times higher than control group (P<0.05). Conclusion: Serum magnesium decreased is an independent risk factor of CAD complicated with diabetes, which can be used as a biomarker of reference for clinical diagnosis. 2021 Vol. 27 (2): 297-301 [Abstract] ( 149 ) HTML (1 KB)  PDF (1210 KB)  ( 229 ) 301 Effects of Pterygium Excision Combined with Superior Temporal Limbal Stem Cell Transplantation on Postoperative Corneal Astigmatism in Pterygium Patients YANG Junchen, CHENG Tingyu, CHENG Yinong, et al DOI: 10.3969/j.issn.1006-6233.2021.02.027 Objective: To study the efficacy of pterygium excision combined with superior temporal limbal stem cell transplantation in the treatment of pterygium. Methods: A total of 60 pterygium patients admitted to hospital from January 2019 to January 2020 were divided into two groups according to the random number table method, one group was the control group, According to the simple random grouping method, they were randomly divided into two groups (study group and control group with 30 cases each). The control group was treated with pterygium excision alone, while the study group was treated with pterygium excision combined with superior temporal limbal stem cell transplantation. Before and after operation, corneal astigmata, naked eye vision, postoperative corneal wound healing time, total effective rate, complication rate and recurrence rate were compared between the two groups. Results: there was no difference in preoperative corneal astigmatism and naked eye vision between the two groups (P>0.05). The postoperative corneal astigmatism of the two groups was lower than that of the preoperative, and the visual acuity of the naked eye was higher (P<0.05). Compared with the control group, the study group had lower postoperative keratodispersion and recurrence rates, higher naked eye vision and total effective rate, and shorter corneal wound healing time (P<0.05). There was no difference in postoperative complication rate between the two groups (P>0.05). Conclusion: Pterygium excision combined with superior temporal limbal corneal dry cell transplantation is effective in the treatment of pterygium patients, which can effectively reduce the patients' corneal astigmata, improve the level of naked eye vision, and promote the rapid healing of corneal wound, with low complication rate and recurrence rate. 2021 Vol. 27 (2): 301-305 [Abstract] ( 104 ) HTML (1 KB)  PDF (1211 KB)  ( 165 ) 305 Efficacy of Intravenous Thrombolysis with Alteplase on Patients with Acute Ischemic Stroke a Meta-Analysis CHENG Zhirui, LIU Fusheng, WANG Sumei, et al DOI: 10.3969/j.issn.1006-6233.2021.02.028 Objective: To systematically evaluate the efficacy of intravenous thrombolysis with alteplase on patients with acute ischemic stroke. Methods: Electronic database, including CNKI、Wanfang、VIP、CBM、Pubmed、Web of Science、Embase and Cochrane, was systematically search to identify the randomized controlled trial of intravenous thrombolysis intervention for acute ischemic stroke patients. Cochrane Collaboration risk of bias tool was used to assess the quality of included studies. Stata 15.1 and RevMan 5.3 were used to perform data analysis. Results: A total of 28 randomized controlled trial studies (n=2763 patients) were included in the meta-analysis. The results of meta-analysis indicated that compared to control group, intravenous thrombolysis could significantly improve the effective rate (RR:0.95, 95%CI:1.17~1.28, P<0.001)、NIHSS score (SMD:-1.11, 95%CI:-1.41~-0.81, P<0.001)、mRS score (SMD:-0.57, 95%CI:-0.69~-0.45, P<0.001)、BI index (SMD:-0.57, 95%CI:-0.69~-0.45, P<0.001) in patients with acute ischemic stroke. There was no difference in bleeding events between the control group and intravenous thrombolytic therapy(RR:1.18, 95%CI:0.87~1.59, P>0.05).Conclusion: Current evidence suggested that intravenous thrombolysis could significantly improve the neurological deficit in patients with acute ischemic stroke. The above conclusions still need to be further verified by larger sample, high-quality, multi-center randomly controlled trials due to the limit of quality of the included studies. 2021 Vol. 27 (2): 305-312 [Abstract] ( 103 ) HTML (1 KB)  PDF (2273 KB)  ( 142 ) 313 Effects of Ticagrelor and Clopidogrel on Postoperative Complications and Prognosis in Elderly Patients with Acute ST-Segment Elevation Myocardial Infarction After Emergency Percutaneous Coronary Intervention TIAN Feng, BAI Xiaomei, WANG Guidong DOI: 10.3969/j.issn.1006-6233.2021.02.029 Objective: To compare the effects of ticagrelor and clopidogrel on postoperative complications and prognosis in elderly patients (aged ≥ 60 years) with ASTEMI after emergency PCI. Methods: 108 elderly patients with ASTEMI who underwent emergency PCI in our hospital from April 2017 to April 2019 were randomly divided by simple random grouping method into control group receiving loading dose of clopidogrel and study group receiving loading dose of ticagrelor, with 54 cases in each group. Blood flow classification before and after TIMI, postoperative no-reflow and malignant arrhythmia, dyspnea, hemorrhage and MACE3 months after surgery were compared between the two groups. Results: The proportion of TIMI grade 3 (92.59%) in the study group was significantly increased in comparison of the control group (75.93%), and the incidence rate of no-reflow and slow reflow (7.41%) was significantly decreased in comparison of the control group (25.93%) (P<0.05). The incidence rate of stent thrombosis, recurrent myocardial infarction and cardiac death in the study group was significantly decreased in comparison of the control group within 3 months after operation (all P<0.05), but there was no significant difference in the incidence rate of stroke between the two groups (P>0.05). There was no significant difference in the incidence rate of malignant arrhythmia, upper gastrointestinal bleeding and intracranial hemorrhage between the two groups within 3 months after operation (P>0.05). Conclusion: The antithrombotic therapy with ticagrelor in elderly patients with ASTEMI undergoing emergency PCI has definite clinical efficacy and good safety, and can effectively improve the prognosis. 2021 Vol. 27 (2): 313-317 [Abstract] ( 128 ) HTML (1 KB)  PDF (1207 KB)  ( 155 ) 317 Efficacy of Dipyridamole Combined with Gamma Globulin and Aspirin in the Treatment of Kawasaki Disease and its Effects on Immune Function and Coagulation Function of Children Patients WANG Ru, et al DOI: 10.3969/j.issn.1006-6233.2021.02.030 Objective: To investigate the efficacy of dipyridamole combined with gamma globulin (intravenous immunoglobulin, IVIG) and aspirin in the treatment of Kawasaki disease (KD) and its effects on immune function and coagulation function of children patients. Methods: 150 children patients with KD were selected and divided into control group and observation group according to the random number table method, with 75 cases in each group. Control group was treated with aspirin tablets + IVIG, and observation group was treated with dipyridamole on the basis of the above medications, and they were treated for 6~8 weeks. The relief times of clinical symptoms, and peripheral blood T lymphocyte subsets and coagulation function indexes before and after treatment were observed in the two groups. The coronary artery injury after treatment was counted in the two groups. Results: The relief times of fever, diffuse mucosal hyperemia, trunk erythema, cervical lymph node swelling and conjunctival hyperemia in observation group were significantly shorter than those in control group (P<0.05). After treatment, the percentage of peripheral blood CD4+ T lymphocyte subsets and the ratio of CD4+/CD8+ T lymphocyte subsets in the two groups were significantly lower than those before treatment (P<0.05) while the percentage of CD8+ T lymphocyte subsets was significantly higher than that before treatment (P<0.05). The level of plasma fibrinogen (Fbg) in the two groups was significantly lower than that before treatment (P<0.05), and the changes in observation group were significantly greater than those in control group (P<0.05). After treatment, the degree of coronary artery injury in observation group was significantly less than that in control group (P<0.05), and the total incidence rate of coronary artery injury in observation group was significantly lower than that in control group (13.33% vs 40.00%) (P<0.05). Conclusion:Dipyridamole combined with IVIG and aspirin has exact efficacy in treating KD. It can significantly enhance the immune function, improve the coagulation dysfunction, and reduce the coronary artery injury. 2021 Vol. 27 (2): 317-321 [Abstract] ( 151 ) HTML (1 KB)  PDF (1208 KB)  ( 300 ) 321 Analysis of Clinical Efficacy and Safety of Propranolol Combined with Methimazole and Propylthiouracil in Patients with Hyperthyroidism YAO Xiaoyan, XIE Zhouni, ZHOU Li DOI: 10.3969/j.issn.1006-6233.2021.02.031 Objective: To investigate the clinical efficacy of propranolol combined with methimazole and propylthiouracil on hyperthyroidism. Methods: 180 patients with hyperthyroidism were selected as the research objects. The study period was from October 2015 to October 2017. According to a Simple Random Method Using Simple Data, They were divided into a control group (treated with propranolol and propylthiouracil) and observation Sheet Group (given propranolol combined with methimazole treatment), 90 cases in each group, and the total effective rate, free triiodothyronine (FT3), free thyroxine (FT4), and thyroid stimulating hormone ( TSH), osteocalcin (OCN), type I collagen carboxy-terminal peptide β-special sequence (β-CTX) and the incidence of adverse reactions were observed and evaluated. Results: The total effective rate of the observation group was higher than that of the control group (χ2=12.291, P＜0.05). After 3 months of treatment, the observation group's FT3, FT4, OCN, β-CTX were lower than the control group, while TSH was higher than the control group (P<0.05); and the observation group FT3, FT4, TSH, OCN, β-CTX The difference before and after treatment was greater than that of the control group (P<0.05). The incidence of adverse reactions in the observation group was lower than that in the control group (χ2=5.178, P＜0.05). Conclusion: Propranolol combined with methimazole has a good effect in the treatment of patients with hyperthyroidism, which can regulate the level of thyroid hormones and ensure safety. 2021 Vol. 27 (2): 321-326 [Abstract] ( 173 ) HTML (1 KB)  PDF (1218 KB)  ( 419 ) 326 Clinical Effect of Dachaihu Decoction on Acute Pelvic Inflammation and its Effect on Secretory Immunoglobulin A and Serum CRP ZHANG Yamin, ZHANG Liran, et al DOI: 10.3969/j.issn.1006-6233.2021.02.032 Objective: To investigate the clinical effect of Dachaihu Decoction on acute pelvic inflammation and its effect on serum secretory immunoglobulin A(sIgA) and C-reactive protein(CRP). Methods: 144 patients with acute pelvic inflammatory disease from December 2018 to May 2020 were randomly divided into two groups, 72 cases in each group. The control group was treated with cefoxitin sodium and doxycycline hydrochloride. The observation group was treated with Dachaihu Decoction on the basis of the control group. The pelvic effusion, pelvic mass, serum inflammatory factors [CRP, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6)] and sIgA before and after treatment were compared between the two groups. The clinical efficacy and the occurrence of adverse reactions during treatment were recorded and compared between two groups. Results: The effective rate of treatment in the observation group was higher than that in the control group (97.22% vs.84.72%) (P<0.05). Compared with before treatment, the level of pelvic effusion and pelvic mass in the observation group and the control group after treatment were decreased (P<0.05), and the level of pelvic effusion and pelvic mass in the observation group was lower than that in the control group (P<0.05). The levels of CRP, TNF-α and IL-6 in the observation group after treatment were lower than those in the control group, and the levels of sIgA were higher than that in the control group (P<0.05). The rate of adverse reactions in the observation group during treatment was lower than that in the control group (8.33% vs 20.83%)(P<0.05). Conclusion: Dachaihu decoction has better clinical efficacy in the treatment of acute pelvic inflammation, reducing pelvic effusion and pelvic mass size, reducing body inflammatory factors and improving immune function, and reducing the adverse reactions, which is worthy of promotion. 2021 Vol. 27 (2): 326-330 [Abstract] ( 86 ) HTML (1 KB)  PDF (1211 KB)  ( 258 ) 330 Effects of Saccharomyces Boulardii on Symptom Improvement and Serum Inflammatory Factors in Children with Acute Infectious Diarrhea CHEN Dandan, YIN Yangyan, GUI Dongmei DOI: 10.3969/j.issn.1006-6233.2021.02.033 Objective: To study the clinical efficacy of saccharomyces boulardii in the treatment of children with acute infectious diarrhea (AID), and to evaluate its effects on symptom improvement and serum inflammatory factors. Methods: 160 children patients with AID treated in our hospital from April 2017 to April 2019 were selected for the study, and the patients were divided into observation group (odd medical record numbers) and control group (even medical record numbers) using a odd-even number method, with 80 cases in each group. On the basis of routine measures, control group was administrated montmorillonite powder, and observation group was administrated saccharomyces boulardii combined with montmorillonite powder, and they were treated for one week. The effective rate of treatment, symptom improvement, stool frequency traits, intestinal microbial condition, levels of serum inflammatory indexes [interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), hypersensitive C-reactive protein (hs-CRP), interferon-γ (INF-γ)] and occurrence of adverse reactions were compared between the two groups. Results: The effective rate of treatment in observation group was significantly higher than that in control group (92.50% vs 80.00%) (P<0.05). The disappearance times of abdominal pain, vomiting and diarrhea and hospital stay in observation group were significantly lower than those in control group (P<0.05). After treatment, the stool frequency, stool trait score, and levels of IL-6, TNF-α, hs-CRP and INF-γ in the two groups were significantly decreased compared with those before treatment, while the quantity of bacteroides was significantly increased (P<0.05), and the above indexes in observation group were significantly different from those in control group at the same time point (P<0.05). There was no significant difference in incidence rate of adverse reactions between the two groups during treatment (P>0.05). Conclusion: Saccharomyces boulardii has significant clinical efficacy in the treatment of acute infectious diarrhea, and it can effectively improve the symptoms, and reduce the inflammatory response, with good safety and good clinical application value. 2021 Vol. 27 (2): 330-334 [Abstract] ( 82 ) HTML (1 KB)  PDF (1212 KB)  ( 186 ) 334 Efficacy of Hydroxychloroquine Sulfate on Sjogren's Syndrome and Its Effects on Levels of Serum IL-17 ESR CRP and IgG FENG Lin, XU Yangyang, LI Mingyao DOI: 10.3969/j.issn.1006-6233.2021.02.034 Objective: To study the efficacy of hydroxychloroquine sulfate in the treatment of Sjogren’s syndrome (SS) and the effects on levels of serum interleukin-17 (IL-17), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and immunoglobulin G (IgG). Methods: A total of 194 SS patients admitted to our hospital from March 2017 to March 2020 were randomly and evenly divided into observation group and control group, with 97 cases in each group. Both groups were given methylprednisolone on the basis of symptomatic treatment, and observation group was additionally given hydroxychloroquine sulfate during treatment, and the treatment lasted 3 months. The treatment effects and adverse reactions were observed in the two groups, and the Schirmer Test (ST), salivary flow rate (SFR) and levels of serum IL-17, ESR, CRP and IgG were detected before and after treatment. Results: The treatment effects in observation group were significantly better than those in control group (P<0.05), and the effective rates of the two groups were 86.60% and 75.26% (P<0.05). There were no significant differences in ST and SFR between the two groups before treatment (P>0.05). After treatment, ST and SFR were significantly increased (P<0.05), and the differences of ST and SFR in observation group after treatment were higher than those in control group (P<0.05). There were no significant differences in the levels of serum IL-17, ESR, CRP and IgG between the two groups before treatment (P>0.05). After treatment, the levels of serum IL-17, ESR, CRP and IgG were significantly reduced (P<0.05), and the differences of IL-17, ESR, CRP and IgG before and after treatment in observation group were higher than those in control group (P<0.05). The incidence rates of adverse reactions in observation group and control group were 16.49% and 12.37% respectively (P>0.05). Conclusion: Hydroxychloroquine sulfate in the adjuvant treatment of SS can effectively reduce the levels of serum IL-17, ESR, CRP and IgG, thereby improving the symptoms and enhancing the treatment effects. 2021 Vol. 27 (2): 334-339 [Abstract] ( 115 ) HTML (1 KB)  PDF (1230 KB)  ( 173 ) 339 Comparison on Effects of Two Drugs in Treatment of Children with Acute Respiratory Infection on Serum CRP PA and IL-6 Levels REN Bo, LI Ruili, YUAN Xiaofeng DOI: 10.3969/j.issn.1006-6233.2021.02.035 Objective: To compare the effects of cefixime and cefaclor in treatment of children with acute respiratory infection (ARI) on levels of serum C-reactive protein (CRP), prealbumin (PA) and interleukin-6 (IL-6). Methods: During the period from July 2018 to June 2019, 78 ARI children who were treated in the hospital were enrolled as research subjects. They were divided into control group and observation group with random number table, 39 cases in each group. The observation group and control group were treated with cefaclor and bromhexine hydrochloride for suspension and cefixime for suspension, respectively. The curative effect and disappearance time of clinical symptoms, levels of serum CRP, PA, procalcitonin (PCT), IL-6, interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) before and after treatment were compared. The gastrointestinal reactions, adverse reactions such as pus embolus and inflammation during treatment in both groups were statistically analyzed. Results: The total response rate of treatment in observation group was higher than that in control group (94.87% vs 79.49%) (P<0.05), while disappearance time of clinical symptoms was shorter than that in control group (P<0.05). The difference of serum CRP,PA,PCT level and IL-6、TNF-α level in the observation group before and after treatment was greater than that in the control group (P<0.05). Conclusion: Compared with cefixime for Suspension, Cefaclor and Bromhexine Hydrochloride for Suspension is more effective in treatment of ARI children, which can shorten disappearance time of clinical symptoms, and relieve inflammation response. 2021 Vol. 27 (2): 339-343 [Abstract] ( 100 ) HTML (1 KB)  PDF (1205 KB)  ( 239 ) 343 Diagnostic Value of FQ-PCR Combined with IGRA for FGT in Menstrual Blood Mycobacterium Tuberculosis CHEN Hongmei, WANG Qi, FANG Zhe, et al DOI: 10.3969/j.issn.1006-6233.2021.02.036 Objective: To explore the diagnostic value of fluorescent quantitative PCR (FQ-PCR) detection of mycobacterium tuberculosis in menstrual blood combined with interferon gamma release assay (IGRA) for female genital tuberculosis (FGT). Methods: A total of 156 cases of tubal infertility and recurrent abortion admitted to our hospital from July 2016 to September 2019 were selected for etiological examination, fluorescence quantitative PCR detection of Mycobacterium tuberculosis in menstrual blood and detection of interferon gamma release test. The patients were divided into 2 groups with the gold standard of endoscopic findings and pathological results. The rate of missed diagnosis was analyzed in both solo and combined detection. Results: The rate of missed diagnosis in combined detection was 14.29%, which was lower than 42.86% of PCR detection alone and 52.38% of interferon-γ release test (P<0.05).The rate of missed diagnosis in combined detection was 11.76%, which was lower than 35.29% of PCR detection alone and 52.94% of γ-interferon release test (P<0.05). Conclusion: As the early screening methods of FGT in tubal infertility and recurrent abortion, the detection of mycobacterium tuberculosis in menstrual blood by fluorescence quantitative PCR and interferon gamma release test have a certain rate of missed detection. The combined detection of the two diagnostic methods can effectively reduce the rate. 2021 Vol. 27 (2): 343-348 [Abstract] ( 83 ) HTML (1 KB)  PDF (1233 KB)  ( 305 )

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