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2020 Vol. 26, No. 10 Published: 2020-10-31 1585 Effects of β-carboline Alkaloids on SGC-7901 Tumor Bearing Mice through FAK/PI3K/AKT/mTOR Pathway FAN Yuxiang, ZENG Fanye, ZHANG Hongliang DOI: 10.3969/j.issn.1006-6233.2020.10.001 Objective: To investigate the effect of β-carboline alkaloids extracted from Peganum harmala L. on the expression of FAK, PI3K, AKT, mTOR, Bcl-2 and Bax in transplanted tumor tissue of human gastric cancer SGC-7901 mice. Methods: The transplanted tumor model was established in mice. 50 Kunming mice were randomly divided into 5 groups: blank control group, 5-fluorouracil group, low-, medium-and high-dose β-carboline alkaloids groups, with 10 mice in each group. The tumor inhibition rate was calculated, the pathological changes of tumor tissues were detected by HE staining, the apoptosis of tumor cells was detected by TUNEL method, the mRNA expression of FAK, PI3K, Akt, mTOR, Bcl-2 and Bax were detected by RT-PCR The expression of FAK, PI3K, Akt, mTOR, Bcl-2 and Bax were detected by blot and immunohistochemistry. Results: The middle and high dose of β-carboline alkaloids can inhibit the growth of SGC-7901 mice tumor and reduce the tumor weight (P<0.01), which is better than the low dose of β-carboline alkaloids (P<0.05). He staining and TUNEL results showed that β-carboline alkaloids could damage the gastric tumor cells and induce apoptosis. RT-PCR, Western blot and immunohistochemistry showed that the middle and high doses of β-carboline alkaloids could significantly reduce the expression of FAK, PI3K, AKT, mTOR and Bcl-2 protein (P<0.01), and increase the expression of Bax protein (P<0.01), which was better than the low doses of β-carboline alkaloids (P<0.05) and the difference was statistically significant. Conclusion: The inhibitory effect of β-carboline alkaloids on gastric cancer is related to the decrease of Bcl-2, FAK, PI3K, AKT, mTOR protein expression and the increase of Bax protein expression, which indicates that β-carboline alkaloids may be a potential therapeutic drug for gastric cancer. 2020 Vol. 26 (10): 1585-1590 [Abstract] ( 145 ) HTML (1 KB)  PDF (2151 KB)  ( 284 ) 1591 The Role and Mechanism of Vitamin D in Regulating Vascular Endothelial Growth Factor in Neonatal Rats with Broncho Pulmonary Dysplasia ZHAO Ting, WANG Yanmei, WANG Le DOI: 10.3969/j.issn.1006-6233.2020.10.002 Objective: To study the effect of VEGF regulated by vitamin D (VD) and/or VD receptor (VDR) on bronchopulmonary dysplasia (BPD) in neonatal rats. Methods: BDP neonatal rats were constructed and divided neonatal rats into control group and BDP group. Rat lung epithelial type Ⅱ cells (RLE-6TN) were cultured, and divided into VDR overexpression group (Le-VDR lentivirus infection group), VDR overexpression control group (Le-Ctrl group), VDR knockdown group (Sh-VDR infection group), VDR knockdown control group (Sh-Ctrl group), VD treatment alone group, VEGF-A silencing group (siVEGF-A group), and Le-VDR and siVEGF-A co-treatment group (Le-VDR + siVEGF-A group). The regulated effect of VD and VDR on lung cells was confirmed by CCK-8 detection, flow cytometry, wound healing detection and in vitro adhesion detection. VDR and VEGF-A mRNA were detected by RTqPCR, and VDR and VEGF-A protein were detected by Western blot. Results: Compared with the control group, the VD level and VDR expression in BPD neonatal rats were downregulated (P<0.01); overexpression of VDR in RLE-6TN cells increased the cell proliferation, migration, and adhesion ability (all P<0.01), and the apoptosis rate were decreased (P<0.01); however, the effect of knocking down VDR in cells was opposite. VD stimulation inhibited cell apoptosis and increased cell proliferation, migration and adhesion (P<0.05); VDR overexpression positively regulated vascular endothelial growth factor (VEGF)-A (P<0.05); Moreover, VEGF-A and VDR were co-localized in the lung tissue and cells of BPD rats; furthermore, the expression levels of VEGF-A in lung tissue of BPD neonatal rats were decreased (P<0.05). Conclusion: VD inhibits the development of BPD by up-regulating the expression of VEGF-A. 2020 Vol. 26 (10): 1591-1596 [Abstract] ( 137 ) HTML (1 KB)  PDF (2026 KB)  ( 284 ) 1596 Diagnosis Value of Combined Detection of Serum TNF-α NSE and MCP-1 in Early Sepsis-related Encephalopathy MENG Zhenfa, LI Yiping, TAN Demin, et al DOI: 10.3969/j.issn.1006-6233.2020.10.003 Objective: To detect serum levels of tumor necrosis factor-α (TNF-α), neuron-specific enolase (NSE), monocyte inflammatory protein-1 (MCP-1) in patients with sepsis-associated encephalopathy (SAE), and to explore the values of TNF-α, NSE and MCP-1 in the early diagnosis of SAE. Methods: 178 patients admitted to our hospital from July 2016 to September 2019 and finally diagnosed as sepsis were selected as the study subjects. Among them, 82 patients without associated encephalopathy (sepsis group) and 96 patients with associated encephalopathy (SAE group). The serum levels of TNF-α and MCP-1 were detected by ELISA and automatic chemiluminescence immunoassay, the correlations between the levels of TNF-α, NSE, MCP-1 and acute physiology and chronic health evaluation scoring system Ⅱ(APACHE Ⅱ), sequential organ failure assessment(SOFA) score were analyzed, and the diagnostic values of TNF-α, NSE and MCP-1 for SAE were analyzed. Results: There was no significant difference in the ratio of male to female, age between the two groups (P>0.05). TNF-α, NSE, MCP-1, APACHE II, SOFA score and 28-day mortality in SAE group were significantly higher than those in sepsis group (P<0.05). Serum TNF-α was positively correlated with NSE, MCP-1, NSE and MCP-1 in SAE patients, and TNF-α, NSE and MCP-1 were positively correlated with APACHE II and SOFA score (P<0.05). The AUCs of TNF-α, NSE and MCP-1 in the diagnosis of SAE were 0.912 (95% CI: 0.869-0.954), 0.795 (95% CI: 0.730-0.861), 0.869 (95% CI: 0.818-0.920), and the truncation values were 6.289 pg/mL, 8.429 ng/mL and 62.265 pg/mL, respectively, the specificities were 86.5%, 78.1% and 77.1%, and the sensitivities were 82.9%, 70.7% and 79.3%. The AUC of the three combination in the diagnosis of SAE patients was 0.978 (95% CI: 0.960-0.995), the specificity was 88.5%, and the sensitivity was 98.8%. Conclusions: TNF-α, NSE and MCP-1 are closely related to the occurrence of SAE and may be involved in the pathogenesis of SAE. The combined detection of TNF-α, NSE and MCP-1 has a good predictive value for the early diagnosis of SAE. 2020 Vol. 26 (10): 1596-1600 [Abstract] ( 121 ) HTML (1 KB)  PDF (1382 KB)  ( 281 ) 1600 Study on the Role and Mechanism of Beclin1-Regulated Autophagy in Embryo Fragment Formation NIU Huanfu, NIU Shufang, WEI Zefeng, et al DOI: 10.3969/j.issn.1006-6233.2020.10.004 Objective: To study the role and possible mechanism of beclin1-regulated autophagy in embryo fragment formation. Methods: A total of 240 patients who underwent in vitro fertilization of eggs in our hospital from July 2016 to October 2019 were selected. Embryo (3PN) was selected and subdivided into the following four groups according to different fragment contents: low fragment group (fragment proportion <10%), medium and low fragment group (25%> fragment proportion ≥10%), medium and high fragment group (50%> fragment proportion ≥25%), and high fragment group (fragment proportion ≥50%), with 60 cases in each group. The selected embryos were cultured in G2 blastocyst medium and interferon (40ng/mL) interferon was used for 36h. Beclin1, PI3K, LC3B, mTOR and AKT were quantitatively determined by western blot. Results: Before and after interference, Beclin1 protein, PI3K protein, LC3B protein, mTOR protein and AKT protein content in the four groups were significantly increased, with statistically significant differences (P<0.05). Before interference, the contents of the six proteins were compared, from high to low: low fragment group, medium and low fragment group, medium and high fragment group, and high fragment group, with statistically significant differences (P<0.05). After infection, the contents of Beclin1 protein, PI3K protein and LC3B protein in the four groups were significantly different (P<0.05). Beclin1 was significantly correlated with PI3K, LC3B, mTOR and AKT (P<0.05). Conclusion: A high proportion of embryo fragments will inhibit Beclin1, PI3K, LC3B, mTOR and AKT protein content. Beclin1's regulation of autophagy may produce interferon-like effects, thus affecting the expression of related channel proteins. 2020 Vol. 26 (10): 1600-1604 [Abstract] ( 122 ) HTML (1 KB)  PDF (1197 KB)  ( 365 ) 1604 Relationship between Levels of Serum Hmga1 CK19 and CA125 and the Severity of Cervical Cancer KANG Yongjun, LI Min, ZHANG Xiaohui, et al DOI: 10.3969/j.issn.1006-6233.2020.10.005 Objective: To study the relationship between levels of serum high mobility proteina1 (Hmga1), cytokeratin (CK) 19 and carbohydrate antigen (CA) 125 and the severity of cervical cancer to provide bases for clinical diagnosis and evaluation of the condition. Methods: The clinical data of 70 patients with cervical cancer (cervical cancer group) diagnosed in the hospital from January 2019 to June 2019 were retrospectively analyzed. The clinical data of 40 patients with cervical intraepithelial neoplasia (CIN) (precancerous lesion group) discovered during gynecologic tumor screening and 30 healthy women (control group) were collected for comparison. The levels of hmga1, CK19 and CA125 in serum of the three groups were compared, and their relationship with the severity of cervical cancer was analyzed. Results: The levels of hmga1, CK19 and CA125 in serum showed cervical cancer grouP> precancerous lesion grouP> control group (P<0.05). In cervical cancer group, there were no significant differences in levels of serum hmga1, CK19 or CA125 between patients with different tumor diameters and pathological types (P>0.05), while levels of serum hmga1, CK19 and CA125 were significantly higher in stage III~IV than in stage I~II, significantly higher in patients with moderate and poor differentiation than in patients with high differentiation, and significantly higher in patients with lymph node metastasis than in patients without (P<0.05). Conclusion: The levels of hmga1, CK19 and CA125 in serum of patients with cervical cancer are significantly increased, and are closely related to stage, differentiation and metastasis. They can be used as auxiliary means for clinical diagnosis of cervical cancer and assessment of the severity of the disease. 2020 Vol. 26 (10): 1604-1608 [Abstract] ( 143 ) HTML (1 KB)  PDF (1191 KB)  ( 379 ) 1608 Expressions of AP-2α and ITGB1 in Cervical Squamous Cell Carcinoma and The Correlation with Prognosis LI Bo, WU Liqun, FANG Lian, et al DOI: 10.3969/j.issn.1006-6233.2020.10.006 Objective: To analyze the relationships between AP-2α and ITGB1 expressions and prognosis of cervical squamous cell carcinoma. Methods: 93 cases of cervical squamous cell carcinoma were selected from October 2013 to December 2016 in our hospital, and 93 cases of hysteromyoma patients who needed total hysterectomy at the same time were selected as control. Cervical squamous cell carcinoma and normal cervical tissue were collected during operation, the expression levels of AP-2α and ITGB1 were detected by immunohistochemical staining, the relationship between the two levels and clinicopathological features, prognosis of cervical squamous cell carcinoma were analyzed, and Cox regression model was used to analyze the risk factors of adverse prognosis in patients with cervical squamous cell carcinoma. Results: The positive expression rate of AP-2α protein in cervical squamous cell carcinoma was significantly lower than that in normal cervical tissue, and the positive expression rate of ITGB1 protein was significantly higher than that in normal cervical tissue (P<0.05). The expression levels of AP-2α and ITGB1 were correlated with myometrial invasion, FIGO stage and lymph node metastasis (P<0.05). There was a negative correlation between AP-2α and ITGB1 expressions in cervical squamous cell carcinoma (r=-0.582, P<0.05). The 3-year overall survival rate of AP-2α positive group was significantly higher than that of AP-2α negative group, and the 3-year overall survival rate of ITGB1 positive group was significantly lower than that of ITGB1 negative group (P<0.05). FIGO stage III, lymph node metastasis, AP-2α negative and ITGB1 positive were independent risk factors for adverse prognosis of cervical squamous cell carcinoma (all P<0.05). Conclusion: Compared with normal cervical tissues, the expression of AP-2α in cervical squamous cell carcinoma is decreased, the expression of ITGB1 is increased. Both of them are associated with myometrial invasion, FIGO staging, lymph node metastasis and survival rate of patients with cervical squamous cell carcinoma. They are independent risk factors for adverse prognosis of patients with cervical squamous cell carcinoma. They may be used as markers for prognosis evaluation. 2020 Vol. 26 (10): 1608-1613 [Abstract] ( 99 ) HTML (1 KB)  PDF (1408 KB)  ( 311 ) 1613 Effects of Anti-bronchial Syrup Combined with Glucocorticoid on NF-κB Signaling Pathway in Model Rats with Cough Variant Asthma GUO Xin, ZHUGE Shurui, QI Xusheng DOI: 10.3969/j.issn.1006-6233.2020.10.007 Objective: To study the effects of anti-bronchial syrup combined with glucocorticoid on NF-κB signaling pathway in model rats with cough variant asthma. Methods: 50 SD rats were selected and randomly divided into control group, model group, anti-bronchial syrup group, glucocorticoid group and combined group, with 10 rats in each group. On the 1st day of the experiment, the other four groups except control group were injected intraperitoneally with ovalbumin (OVA) and aluminum hydroxide, and they were treated for three times with once/7days for rat asthma model construction. On the 21st day of the experiment, they were given ovalbumin inhalation for atomization stimulation, and control group was stimulated with normal saline for 3 times/week with 30 min/time. Each drug-administered group was pretreated with drugs at 30 min before atomization stimulation, and control group was treated with the same amount of normal saline. 3 weeks after stimulation, HE staining was used to observe the pathological changes of the lung tissue, and the thickness of airway wall and the thickness of smooth muscle were measured. Western blot was used to detect the expression levels of IκB, NF-κB, MMP-9 and VEGF proteins in rat lung tissue. Results: HE staining showed that combined group could significantly improve the airway wall epithelial tissue necrosis and shedding, and a large number of inflammatory cell infiltration. The analysis of airway wall and smooth muscle thickening showed that the airway wall and smooth muscle thickening in the other four groups were significantly thickened compared with control group (P<0.05). Compared with model group, the degree of tissue thickening in drug-treated groups was significantly decreased, and the degree in combined group was significantly lower than that in single drug groups (P<0.05). Compared with control group, the expression level of IκB protein was significantly decreased in the other four groups, and the expression levels of NF-KB, MMP-9 and VEGF were significantly increased (P<0.05). Compared with model group, the expression level of IKB protein in single drug groups and combined group was significantly increased, and the expression levels of NF-KB, MMP-9 and VEGF were significantly decreased (P<0.05), and the changes of protein expression in combined group were significantly higher than those in single drug groups (P<0.05). Conclusion: Kangzhi syrup combined with dexamethasone can reduce the hydrolysis of I κ B protein, decrease the release of NF-κ B protein, down regulate the expression of MMP-9 and VEGF, and inhibit the airway remodeling of cough variant asthma rats. 2020 Vol. 26 (10): 1613-1617 [Abstract] ( 104 ) HTML (1 KB)  PDF (1535 KB)  ( 471 ) 1617 The Relevance Between the Expressions of MiR-335-5p and Transcription Factor ATF2 in Cervical Cancer RAN Wei, LIAO Ping, LIU Xinglan, et al DOI: 10.3969/j.issn.1006-6233.2020.10.08 Objective: To investigate the expressions of microRNA-335-5p (miR-335-5p) and activated transcription factor 2 (ATF2) in cervical cancer and their relationship with clinicopathological characteristics of cervical cancer patients. Methods: 72 patients with cervical cancer who underwent surgical treatment in our hospital from August 2017 to October 2019 were selected as the experimental group, 65 patients with uterine leiomyoma who underwent surgical treatment in the same period were selected as the control group, and cervical tissues of cervical cancer and uterine leiomyoma patients who underwent surgical resection were taken as the research samples. Real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the expression of miR-335-5p, ATF2 mRNA and matrix metalloproteinase 2(MMP2) mRNA; immunohistochemistry was used to detect the positive expression rate of ATF2 and MMP2 protein; the relationships between the expression of miR-335-5p, ATF2 and the clinicopathological characteristics of cervical cancer were analyzed; the factors affecting cervical cancer were analyzed by logistic regression analysis; and the correlation of expression of mir-335-5p, Atf2 and MMP2 was analyzed. Results: The expression levels of miR-335-5p, ATF2 mRNA and MMP2 mRNA in the experimental group were significantly higher than those in the control group (P<0.05). The positive expression rate of ATF2 and MMP2 protein in the experimental group was significantly higher than that in the control group (P<0.05). The expression levels of miR-335-5p, ATF2 and MMP2 in cervical cancer tissues were correlated with FIGO stage, depth of invasion and lymph node metastasis (P<0.05). Logistic multivariate regression analysis showed that miR-335-5p, ATF2 and MMP2 were risk factors for cervical cancer. There was a positive correlation between the expression of miR-335-5p, Atf2 and MMP2 in cervical cancer (P<0.05). Conclusion: The expressions of miR-335-5p and ATF2 are high in cervical cancer tissues. Both of them are related to FIGO stage, depth of invasion and lymph node metastasis, and they may be biological indicators affecting the occurrence and development of cervical cancer. 2020 Vol. 26 (10): 1617-1623 [Abstract] ( 81 ) HTML (1 KB)  PDF (1555 KB)  ( 460 ) 1623 Detection of Serum SIL-2R Level and T lymphocyte Subsets Levels and The Correlation in Secretory Otitis Media CHEN Xuan, LI Dongdong, SHI Yan DOI: 10.3969/j.issn.1006-6233.2020.10.009 Objective: To study the correlation between serum soluble interleukin-2 receptor (SIL-2R) level and T lymphocyte subsets levels in secretory otitis media (SOM). Methods: A total of 235 patients with SOM admitted to the hospital from March 2015 to December 2019 were selected as observation group. Another 100 healthy people who underwent physical examination in our hospital at the same time period were selected as control group. The levels of SIL-2R and T lymphocyte subsets-related indexes were measured in the two groups. The correlation between SIL-2R and T lymphocyte subsets was analyzed by Pearson correlation. Results: The levels of CD3, CD4 and CD4 / CD8 in the observation group were significantly lower than those in the control group (P<0.05), and the level of CD8 in the observation group was significantly higher than that in the control group (P<0.05). The level of SIL-2R in the observation group was significantly higher than that in the control group (P<0.05); the level of serum SIL-2R was significantly negatively correlated with CD3, CD4, CD4 / CD8 (P<0.05), and positively correlated with CD8 (P<0.05). Conclusions: There is a certain correlation between SIL-2R and T lymphocyte subsets levels. 2020 Vol. 26 (10): 1623-1625 [Abstract] ( 103 ) HTML (1 KB)  PDF (1150 KB)  ( 297 ) 1625 Comparison of Different Doses of Sufentanil and Fentanil on Hemodynamics and Myocardial Protection During Perioperative Period of Cardiac Valve Replacement ZHU Tongtong, WANG Chaoxia, QI Yue DOI: 10.3969/j.issn.1006-6233.2020.10.010 Objective: To analyze the effects of different doses of sufentanil and fentanil on hemodynamics and myocardial protection during perioperative cardiac valve replacement.Methods: A total of 100 patients undergoing elective heart valve replacement in hospital from January 2018 to December 2019 were selected and randomly divided into group A (fentanyl 7μg/kg), group B (sufentanil 1μg/kg), group C (Sufentanil 1.5μg/kg), 25 patients in Group D (Sufentanil 2 μg/kg), observe the four groups of patients 5 minutes before anesthesia induction (T0), 10 minutes after anesthesia induction (T1), intubation Heart rate (HR), mean arterial pressure (MAP), cardiac troponin (cTnI) at 1 min (T2), 3 min (T3) after intubation, 5 min (T4) after intubation, 10 min (T5) after intubation ), Creatine Phosphokinase Isozyme (CK-MB).Results: There were statistically significant differences in the interaction effects of HR, MAP, cTnI and CK-MB levels between the four groups, time points, and time points with the groups (P<0.05).Comparison of HR, MAP, cTnI and CK-MB levels between the four groups at T0 and T1 showed no statistically significant difference (P>0.05);T2 time point, the HR, MAP, CK-MB level comparison between the four groups, the difference Statistically significant (P <0.05); T3 time point, MAP, cTnI, CK-MB levels between the four groups, the difference was statistically significant (P <0.05); T4 time point, MAP, cTnI between the four groups Compared with CK-MB levels, the difference was statistically significant (P <0.05); at T5, the HR, MAP, cTnI, and CK-MB levels between the four groups were statistically significant (P <0.05).Conclusion: Sufentanil and fentanyl in cardiac valve replacement have similar effects on the hemodynamics of patients, but the myocardial protection of sufentanil is higher than that of fentanyl, and the effects between different doses are similar, suggesting that Reduce the amount of sufentanil used. 2020 Vol. 26 (10): 1625-1630 [Abstract] ( 90 ) HTML (1 KB)  PDF (1208 KB)  ( 274 ) 1630 The Correlation of Serum IL-17 and TLR4 in Patients with COPD and Their Correlation with FEV1%pred mMRC Grading and CAT Score SUN Yin, WEI Haiyan DOI: 10.3969/j.issn.1006-6233.2020.10.011 Objective: To investigate the expression of serum interleukin-17 (IL-17) and toll-like receptor 4 (TLR4) in patients with chronic obstructive pulmonary disease (COPD) and their correlation with the forced expiratory volume in the first second as a percentage of the predicted value (FEV1%pred), the modified British medical research council (mMRC grading), and the COPD Assessment Test (CAT) score.Methods: The levels of serum IL-17 and TLR4 in patients in three groups [COPD group, healthy smoking control group (C1), healthy non-smoking control group (C2), 80 cases in each group] were measured by ELISA method, and analyzed their relevance with FEV1%pred, mMRC grading, and CAT score. Results: The levels of serum IL-17 and TLR4 in patients of COPD group were significantly higher than those in health controls (C1 group and C2 group) (P<0.05). Serum IL-17 in group C1 was significantly higher than that in group C2 (P<0.05); serum TLR4 in group C1 was significantly lower than that in group C2 (P<0.05). The levels of serum IL-17 and TLR4 in the patients of COPD group were negatively correlated with FEV1%pred (P<0.05). The levels of serum IL-17 and TLR4 in COPD group were positively correlated with mMRC grading and CAT score (P<0.05). There was a positive correlation between the serum IL-17 level and TLR4 level in the patients of COPD group (P<0.05). Conclusion: The levels of IL-17 and TLR4 in patients with chronic obstructive pulmonary disease were significantly up-regulated, and they were positively correlated with the severity of the disease. 2020 Vol. 26 (10): 1630-1633 [Abstract] ( 102 ) HTML (1 KB)  PDF (1169 KB)  ( 377 ) 1634 Endoscopic Surgery For Cerebral Hemorrhage in Basal Ganglia Region by Transfrontal LI Peng, ZHANG Yi, YANG Xiao, et al DOI: 10.3969/j.issn.1006-6233.2020.10.012 Objective: To evaluate the effect of neuroendoscopic-assisted transfrontal approach in the treatment of intracerebral hemorrhage in Basal Ganglia. Methods: Analysis 86 cases of Basal ganglia region hemorrhage patients who underwent endoscopic surgery by transfrontal or microscopes surgery by transtemporal,including intraoperative,postoperative and prognosis,and related data. Endoscopic transfrontal group (observation group) 43 Cases with Microscopes surgery group (control group) 43 cases. Results: The clearance rate of Hematoma in Endoscopic transfrontal group is 89±7.3%, in microscopes surgery group is 73±6.8%.The clearance rate of Hematoma, time for surgery,amount of blood lost during surgery,biomarkers in observation group are better than the control group. The score of NIHSS、BI、mRS in observation group is better than the control group. Conclusion: Neuroendoscopic-assisted transfrontal approach for hypertensive cerebral hemorrhage can effectively improve the therapeutic effect, reduce the operation time and intraoperative bleeding, improve the prognosis of patients. 2020 Vol. 26 (10): 1634-1637 [Abstract] ( 79 ) HTML (1 KB)  PDF (1158 KB)  ( 265 ) 1637 Prognostic Factors of Patients with Cerebral Infarction in Emergency Thrombolysis REN Chao, ZHAO Jun, ZHANG Xiaoming DOI: 10.3969/j.issn.1006-6233.2020.10.013 Objective: To explore the prognostic factors and predictive value of thrombolytic patients with emergency cerebral infarction. Methods: The data of 248 patients with acute cerebral infarction treated by thrombolysis in hospital from August 2017 to August 2019 were analyzed and divided into poor prognosis group (n=104) and good prognosis group (n=144) according to the patients' neurological recovery. All patients received intravenous thrombolysis. The risk factors were observed and analyzed that may affect the prognosis of thrombolytic patients with emergency cerebral infarction, and perform multivariate logistic regression. And do some prognostic value analysis on the prognosis of some factors/indicators. Results: The results of univariate analysis showed that the onset-treatment time, incidence of diabetes history, homocysteine, blood glucose, and systolic blood pressure before intravenous thrombolysis in the poor prognosis group were significantly different from those in the good prognosis group (P<0.05) ; Multivariate regression results show that: onset-treatment time index, NIHSS index, previous diabetes history index, homocysteine index, blood glucose index, and systolic blood pressure index before intravenous thrombolysis are the significant factors affecting the poor prognosis of patients with acute cerebral infarction thrombolysis Influencing factors (P<0.05). Three of these indicators (homocysteine, blood glucose, and systolic blood pressure before intravenous thrombolysis) were analyzed by ROC, which showed that it had a certain predictive evaluation value for the prognosis of patients with acute cerebral infarction thrombolysis. The AUC (0.95CI) were: 0.749 (0.595~0.942), 0.783 (0.666~0.921), 0.678 (0.548-0.839). The combined application has a higher value in predicting the prognosis of patients with acute thrombolysis of cerebral infarction, and its AUC (0.95CI) is 0.819 (0.637~0.996). Conclusion: There are many independent risk factors that influence the prognosis of patients with acute cerebral infarction thrombolysis. Clinicians need to pay close attention to the onset-treatment time, NIHSS, previous medical history of diabetes, homocysteine, blood glucose, systolic blood pressure before intravenous thrombolysis and other factors. 2020 Vol. 26 (10): 1637-1642 [Abstract] ( 114 ) HTML (1 KB)  PDF (1252 KB)  ( 295 ) 1642 Expression of DJ-1 in Prostate Cancer and its Relationship with Curative Effect of Neoadjuvant Endocrine Therapy WEI Gaomeng, TANG Yujin, HUANG Yongping DOI: 10.3969/j.issn.1006-6233.2020.10.014 Objective: To explore the expression of DJ-1 in prostate cancer and its relationship with curative effect of neoadjuvant endocrine therapy. Methods: The cancer tissues and para-cancerous fresh tissues of 98 patients who were confirmed with prostate cancer by puncture in the hospital from January 2015 to April 2016 were collected. The paraffin blocks of benign prostatic hyperplasia (BPH) tissues from 98 BPH patients were collected. The expression level of DJ-1 protein in each tissue was detected by immunohistochemistry. The expression level of DJ-1 protein in prostate cancer patients before and after neoadjuvant endocrine therapy was detected. χ2 test was applied to analyze the relationship between DJ-1 and clinical pathological parameters of prostate cancer, curative effect of neoadjuvant endocrine therapy. Kaplan-Meier survival curve and Log-rank method were applied to detect the relationship between DJ-1 and biochemical recurrence-free survival (BRFS). Results: The expression level of DJ-1 protein in BPH tissues was significantly lower than that in prostatic cancer tissues (P<0.05). There was no significant difference in the above index between BPH tissues and para-cancerous tissues (P>0.05). The expression level of DJ-1 protein in para-cancerous tissues was significantly lower than that in prostatic cancer tissues (P<0.05). After neoadjuvant endocrine therapy, positive expression of DJ-1 was decreased (P<0.05). There were significant differences in Gleason staging, lymph node metastasis, TNM staging and clinical curative effect between patients with positive DJ-1 and negative DJ-1 (P<0.05). The 3-year BRFS rates in DJ-1 negative (-), weak positive (+), moderate positive (++) and strong positive (+++) group were 93.33%, 87.50%, 74.07% and 70.27%, respectively. The differences among all groups were statistically significant detected by Log-rank test (P<0.05). Conclusion: The expression of DJ-1 is associated with tumor differentiation, lymph node metastasis, TNM staging, clinical curative effect of neoadjuvant endocrine therapy and 3-year BRFS rate, which is of certain predictive value for prognosis of patients with prostate cancer. 2020 Vol. 26 (10): 1642-1647 [Abstract] ( 67 ) HTML (1 KB)  PDF (1484 KB)  ( 268 ) 1647 The Association Between the Expression of lncRNA SNHG20 and Radiosensitivity Prognosis of Breast Cancer WEN Lanxiang, QIN Shiyun, CHEN Lijun DOI: 10.3969/j.issn.1006-6233.2020.10.015 Objective: To detect the expression of lncRNA SNHG20 in breast cancer and normal breast tissue, and to analyze its relationships with radiosensitivity and prognosis of breast cancer. Methods: 119 patients with breast cancer admitted to our hospital from November 2013 to October 2016 were selected as the research objects, with their biopsy cancer tissue being collected, and 119 normal breast tissues retained in our hospital pathological database during the same period were selected as the control group. Real-time fluorescence quantitative (qRT-PCR) was used to detect the expression of lncRNA SNHG20, and then its association with clinicopathological characteristics and radiosensitivity of breast cancer patients were analyzed. Cox regression model was used to analyze the independent risk factors for prognosis of breast cancer patients. Results: The expression level of lncRNA SNHG20 in breast cancer tissue was significantly higher than that in normal breast tissue (P<0.05). The expression of lncRNA SNHG20 in breast cancer tissues was correlated with differentiation, TNM stage, lymph node metastasis and adjuvant chemotherapy (all P<0.05). The remission rate of patients with high expression of lncRNA SNHG20 after radiotherapy was significantly lower than that of patients with low expression of lncRNA SNHG20 (P<0.05). The 3-year overall survival rate of patients with high expression of lncRNA SNHG20 was significantly lower than that of patients with low expression of lncRNA SNHG20 (P<0.05). TNM stage III, lymph node metastasis and high expression of lncRNA SNHG20 were independent risk factors for poor prognosis of breast cancer patients (all P<0.05). Conclusion: LncRNA SNHG20 is highly expressed in breast cancer, and is related to low radiosensitivity, TNM stage, lymph node metastasis and overall survival rate of breast cancer patients. It may be a marker of radiosensitivity and prognosis of breast cancer, and provide a theoretical basis for improving the clinical efficacy and prognosis of breast cancer patients. 2020 Vol. 26 (10): 1647-1651 [Abstract] ( 101 ) HTML (1 KB)  PDF (1242 KB)  ( 281 ) 1652 Correlation Analysis of Serum Total IgE, ECP and HRF Concentrations in Patients with Chronic Urticaria Complicated with Hp Infection KONG Dehua, HUANG Wei, ZHAO Guiping, et al DOI: 10.3969/j.issn.1006-6233.2020.10.016 Objective: To analyze the correlation between serum total immunoglobulin E(IgE), eosinophil cationic protein (ECP) and histamine release factor (HRF) and helicobacter pylori (Hp) infection in urticaria. Methods: 76 patients with urticaria were divided into mild group (13 cases), moderate group (38 cases) and severe group (25 cases) according to the severity of the disease. They were divided into Hp positive group (48 cases) and Hp negative group (28 cases) according to the Hp infection, at the same time, 68 healthy volunteers were collected as control group. The levels of total IgE, ECP and HRF in blood of each group were compared and correlation analysis was carried out. Results: The levels of serum total IgE, ECP and HRF in urticaria group were higher than those in control group (P<0.05). The levels of serum total IgE, ECP and HRF in severe urticaria group were higher than those in moderate group and mild group, and the serum total IgE, ECP and HRF levels in moderate group were higher than those in mild group (P<0.05). The levels of serum IgE, ECP, HRF and HP in HP positive urticaria group were higher than those in HP negative urticaria group (P<0.05). Pearson correlation analysis showed that serum total IgE, ECP, HRF levels were positively correlated with HP (P<0.05). Conclusion: Urticaria infection combined with HP infection can promote the expression of serum total IgE, ECP and HRF, and increase the severity of the disease. 2020 Vol. 26 (10): 1652-1655 [Abstract] ( 101 ) HTML (1 KB)  PDF (1168 KB)  ( 285 ) 1655 Effect of Internal Fixation of Compression Plate and Bridge Combined Internal Fixation on Clinical Efficacy Fracture Healing and Complications of Patients with Fracture of Femoral Shaft TANG Chengjie, LIU Jingjing DOI: 10.3969/j.issn.1006-6233.2020.10.017 Objective: To analyze the clinical value of internal fixation of compression plate and bridge combined internal fixation in the treatment of elderly patients with fracture of femoral shaft. Methods: 159 patients with fracture of femoral shaft treated in our hospital from January 2017 to January 2019 were made the research objects. According to the different surgical methods, 72 patients treated with traditional internal fixation of compression plate were included into the control group, and 87 patients treated with bridge combined internal fixation were included into the observation group. The clinical data of all patients were reviewed retrospectively, and the clinical efficacy, condition during operation and hospitalization were compared between the two groups, as well as fracture healing, hip function and complications. Results: There was no difference in the excellence and good rate of clinical treatment between the two groups (93.06% vs 95.40%) and no difference in length of surgical incision (P>0.05). The operation time, length of hospitalization stay and time of fracture healing in the observation group were shorter than those in the control group. The intraoperative blood loss and postoperative drainage volume were less than those in the control group, and the postoperative pain relief was more obvious than that in the control group. During follow-up, compared with those in the control group, mobility and function of hip joint were better, and the incidence of postoperative complications was lower. The differences were statistically significant (P<0.05). Conclusion: The internal fixation of compression plate and bridge combined internal fixation in the treatment of elderly patients with fracture of femoral shaft both have good clinical effect, but the intraoperative condition of bridge combined internal fixation is better and the recovery of hip joint function is better and the safety is higher. It is worthy of clinical promotion. 2020 Vol. 26 (10): 1655-1659 [Abstract] ( 92 ) HTML (1 KB)  PDF (1184 KB)  ( 249 ) 1659 Clinical Efficacy of Fusion Technology and Coflex Implantation in the Treatment of Senile Degenerative Lumbar Spinal Stenosis GE Xinjiang, et al DOI: 10.3969/j.issn.1006-6233.2020.10.018 Objective: To study the effect of Coflex implantation and total lamina decompression bone grafting fusion and internal fixation in the treatment of senile degenerative lumbar spinal stenosis. Methods: A retrospective analysis was conducted among 62 cases of hospitalized elderly patients with degenerative lumbar spinal stenosis disease from January 2018 to June 2019. Patients were divided into two groups according to the different operation methods, namely the control group and the research group, 31 cases in each. The former were treated with total lamina decompression bone grafting fusion and internal fixation, and the latter were treated with Coflex implantation. Pre- and post-operative data of both groups were compared, including the evaluation values with Japanese Orthopaedic Association (JOA), Visual Analogue Scale (VAS) and intraoperative blood loss, operative time, wound drainage volume, length of hospital stay and incidence rate of complication. Results: JOA score, VAS score, intraoperative blood loss, operation time, wound drainage volume, length of hospital stay and complication rate of the research group were (22.48±2.74), (2.52±0.31), (106.43±17.62) mL, (64.89±8.48) min, (80.52±16.07) mL, (5.48±0.91) d, and (3.23%) respectively. Those of the control group were (20.72±2.41) points, (3.06±0.64) points, (178.25±24.81) mL, (114.72±15.63) min, (136.41±25.26) mL, (8.75±1.52) d, and 9.68%. Compared with the control group, the research group had higher postoperative JOA score, lower VAS score, shorter operative time and hospitalization time, and less intraoperative blood loss and wound drainage (P<0.05).The incidence rate of postoperative complications of the study group was slightly lower than that of the control group, but the difference was not statistically significant (P>0.05). Conclusion: The effect of Coflex implantation in the treatment of senile patients with degenerative lumbar spinal stenosis is better than that of total lamina decompression bone grafting fusion and internal fixation, with high safety and effectiveness. 2020 Vol. 26 (10): 1659-1663 [Abstract] ( 86 ) HTML (1 KB)  PDF (1184 KB)  ( 226 ) 1663 Effect of Laparoscopic Dismembered Pyeloplasty on Perioperative Indexes Postoperative Complications and Prognosis in Children with Hydronephrosis Caused by UPJO ZHOU Enhui DOI: 10.3969/j.issn.1006-6233.2020.10.019 Objective: To study the effect of laparoscopic dismembered pyeloplasty (LDP) on perioperative indexes, postoperative complications and prognosis in children with hydronephrosis caused by ureteropelvic junction obstruction (UPJO). Methods: A retrospective analysis was conducted. 190 children with hydronephrosis caused by UPJO diagnosed and treated in the hospital between September 2016 and September 2019 were selected by convenience sampling. They were divided into the study group (106 cases) and the control group (84 cases) according to the operation methods. The former were treated with LDP and the latter were treated with open pyeloplasty. Observe and compare the perioperative indicators and postoperative complications of the two groups, and evaluate the renal morphology and renal function of the children after surgery. Results: The operation time of the study group was longer than that of the control group. The intraoperative blood loss, length of hospital stay and the dosage of analgesics were less or shorter than those of the control group (P<0.05). The incidence of postoperative complications was significantly lower than that of the control group (5.66% vs 14.19%, P<0.05). The thickness of renal parenchyma and glomerular filtration rates (GFR) in the two groups were increased, while the renal volume and serum creatinine (Scr) levels were decreased in 6 months after operation. Besides, the increase or decrease in study group was greater than that in the control group at 3 months after operation (P<0.05). However, there was no significant difference between the two groups at 6 months after operation (P>0.05). Conclusion: LDP is safe and effective in the treatment of children with hydronephrosis caused by UPJO. It can reduce the intraoperative blood loss and postoperative complications, promote postoperative recovery of renal morphology and renal function, and improve the prognosis. 2020 Vol. 26 (10): 1663-1667 [Abstract] ( 100 ) HTML (1 KB)  PDF (1312 KB)  ( 319 ) 1668 The Study of the Effects of Medication Time and Dose of Oxytocin on the Severity of Neonatal Jaundice ZHU Tong'e, ZHANG Jingjing, LI Xiumin DOI: 10.3969/j.issn.1006-6233.2020.10.020 Objective: To analyze the effect of medication time and dose of oxytocin on the severity of neonatal jaundice. Methods: A total of 1,320 pregnant women who underwent vaginal delivery in Jiaozhou Central Hospital from September 2019 to April 2020 were enrolled. Among them, 570 cases treated with oxytocin before delivery were included in experimental group and 750 oxytocin-free cases were included in control group. The changes of maternal umbilical cord serum total bile acid (TBA) after delivery, and neonatal transcutaneous bilirubin within 4d after birth were compared between the two groups. The incidences of neonatal jaundice in both groups were recorded. Meanwhile, the incidences of neonatal jaundice and transcutaneous bilirubin levels were compared among subgroups of the experimental group (pregnant women in the experimental group were divided into oxytocin use 1 ~ 2 h group, 3 ~ 4 h group and 4 ~ 5 h group according to the medication time of oxytocin, and they were divided into 5 ~ 10U group, 11 ~ 16U group and > 16U group according to the dose of oxytocin used) . Results: The maternal umbilical cord blood TBA levels in experimental group were significantly higher than those in the control group after delivery (P<0.05). On the 1st, 2nd, 3rd and 4th day after birth, neonatal percutaneous bilirubin levels in experimental group were higher than those in control group (P<0.05). The incidences of neonatal pathological jaundice and physiological jaundice and total incidence of jaundice in experimental group were higher than those in control group (P<0.05). In experimental group, the incidences of jaundice and percutaneous bilirubin within 4d after birth in neonates born by puerperae in 3 ~ 4 h group were lower than those born by 1 ~ 2 h group and 4 ~ 5 h group (P<0.05). The incidences of jaundice in neonates born by puerperae in 5 ~ 10 U group and 11 ~ 16 U group were lower than those born by > 16 U group (P<0.05). On the 2nd, 3rd and 4th day after birth, percutaneous bilirubin in neonates born by puerperae in 11 ~ 16 U group and > 16 U group was higher than 5 ~ 10 U group (P<0.05). Conclusion: Prenatal application of oxytocin may increase the risk of neonatal jaundice, and the incidence of neonatal jaundice is relatively higher in puerperae with oxytocin dose higher than 16U. Besides, the effect of oxytocin medication time of 3 ~ 4 h and dose of 11 ~ 16 U is the least. 2020 Vol. 26 (10): 1668-1673 [Abstract] ( 136 ) HTML (1 KB)  PDF (1193 KB)  ( 408 ) 1673 Effect of Suture, Monopolar and Bipolar Electrocoagulation and Ultrasonic Knife in Hemostasis on Ovarian Function and Prognosis of Patients Undergoing Laparoscopic Ovarian Endometriosis Cyst Excision ZHANG Xuegang, LIU Qin, LU Xiaojuan, et al DOI: 10.3969/j.issn.1006-6233.2020.10.021 Objective: To investigate the effect of suture, monopolar and bipolar electrocoagulation and ultrasonic knife in hemostasis on ovarian function and prognosis of patients undergoing laparoscopic excision of endometriosis cyst .Methods: The clinical data of 133 patients with ovarian endometriosis cyst from May 2017 to April 2019 were analyzed retrospectively. Based on the hemostatic methods adopted in routine laparoscopic ovarian endometriosis cyst resection, the patients were divided into suture group (36 cases), monopolar electrocoagulation group (32 cases), bipolar electrocoagulation group (30 cases) and ultrasonic knife group (35 cases). The follicle stimulating hormone (FSH) and the sinusoidal follicle count (AFC) were observed at 1 month, 3 months,6 months and 12 months after operation. And menstrual changes and pregnancy outcomes were recorded in the follow-up 12 months after operation. Results: The level of follicle stimulating hormone (FSH) in the four groups at 1,3,6 and 12 months after operation was gradually increased, and the FSH level in the suture group at 12 months after operation was significantly lower than those in the other groups (P<0.05). The level of sinusoidal follicle count (AFC) decreased in all patients at 1 month after operation, and gradually increased at 3,6 and 12 months after operation. There was a statistical difference in the groups (P<0.05).The number of natural pregnancy cases in the suture group was 30(83.33%), which was significantly higher than that in the monopolar group, the bipolar group and the ultrasonic knife group (P<0.05), and the number of abnormal menstrual cases in the suture group was 3(8.33%), which was significantly lower than that in the other three groups (P<0.05). Conclusion: Monopolar electrocoagulation, bipolar electrocoagulation and ultrasonic knife hemostasis can cause great harm to the ovarian function of patients undergoing laparoscopic ovarian cystectomy, and seriously affect the prognostic effect. 2020 Vol. 26 (10): 1673-1677 [Abstract] ( 109 ) HTML (1 KB)  PDF (1193 KB)  ( 376 ) 1677 Application of Ropivacaine Infiltration Anesthesia Combined with Sufentanil in Labor Analgesia of Primipara ZHANG Jianbo, SU Ran DOI: 10.3969/j.issn.1006-6233.2020.10.022 Objective: To explore the application of ropivacaine intraspinal anesthesia combined with sufentanil in labor analgesia of primipara. Methods: 100 cases of primipara who delivered in our hospital were selected as the research object. The selected time range was from December 2016 to July 2018. They were divided into control group or compound group, 50 cases of primipara in each group. The control group was given ropivacaine intraspinal anesthesia, and the compound group was given ropivacaine intraspinal anesthesia combined with sufentanil. Anesthesia index, duration of labor, visual analogue scale (VAS) scores of 5, 30, 60 and 90 minutes before and after anesthesia, delivery mode and incidence of adverse reactions were compared between the two groups. Results: The onset time of analgesia (7.13±1.97) min, complete block time (12.59±2.34) min and duration of analgesia (153.43±26.57) min in the compound group were significantly shorter than those in the control group (16.45±2.14) min, 24.56±2.81) min and (106.35±31.27) min respectively The time of the third stage of labor (6.71±1.47) min was shorter than that of the control group (532.26±207.34) min, (46.86±10.47) min, (10.13±2.69) min; after anesthesia, the pain of the two groups of parturients was more relieved, but the pain degree of the compound group of parturients was (3.68±0.41), (2.56±0.33), (1.64±0.22), (1.57±0.21) respectively. It was significantly lower than that of the control group (4.76±0.49), (3.14±0.32), (2.58±0.24), (2.46±0.23); the natural delivery rate of 86.00% in the compond group was significantly higher than that of 62.00% in the control group (P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05). Conclusion: The effect of ropivacaine combined with sufentanil on labor analgesia of primipara is better. It can take effect quickly, shorten the time of complete block, prolong the duration of analgesia, shorten the time of the first labor process, the second labor process and the third labor process, reduce the degree of pain of primipara and improve the rate of normal labor. 2020 Vol. 26 (10): 1677-1682 [Abstract] ( 80 ) HTML (1 KB)  PDF (1204 KB)  ( 259 ) 1682 An Analysis of the Diagnostic Value of Transvaginal Ultrasound Combined with Abdominal Ultrasound in Ectopic Pregnancy WU Qing, TANG Rong, CHEN Gang DOI: 10.3969/j.issn.1006-6233.2020.10.023 Objective: To explore the diagnostic value of vaginal ultrasound combined with abdominal ultrasound in the diagnosis of ectopic pregnancy (EP). Methods: A total of 115 patients who were diagnosed as possible EP were selected for the study. All patients underwent transvaginal ultrasound and abdominal ultrasound. The diagnostic results of transvaginal ultrasound, abdominal ultrasound and combined examination were evaluated, and the diagnostic efficacy was calculated, and the diagnostic accuracy rates of each type of EP by combined examination and single examination were compared, and the images of transvaginal ultrasound and abdominal ultrasound were analyzed. Results: The sensitivity and accuracy rate of transvaginal ultrasound were higher than those of abdominal ultrasound (P<0.05), and the sensitivity and accuracy rate of combined examination were higher than those of transvaginal ultrasound or abdominal ultrasound (P<0.05). There was no significant difference in the positive predictive value of each examination alone or in combination (P>0.05), and the negative predictive value of combined examination was higher than that of transvaginal ultrasound or abdominal ultrasound (P<0.05). The accuracy rate of tubal pregnancy and total accuracy rate of combined examination and transvaginal ultrasound were higher than those of abdominal ultrasound (P<0.05), and the accuracy rate of tubal pregnancy and total accuracy rate of combined examination were higher than those of transvaginal ultrasound (P<0.05). The coincidence rate of combined examination and transvaginal ultrasound in the diagnosis of EP was higher than that of abdominal ultrasound (P<0.05). Conclusion: The combination of transvaginal ultrasound and abdominal ultrasound promotes the diagnostic efficacy of EP and has certain clinical application value. 2020 Vol. 26 (10): 1682-1685 [Abstract] ( 84 ) HTML (1 KB)  PDF (1573 KB)  ( 261 ) 1685 Effect of TEVAR on Aortic Remodeling in Patients with Stanford Type B Aortic Dissection at Different Stages LI Jian, TONG Xiwen, WANG Kun, et al DOI: 10.3969/j.issn.1006-6233.2020.10.024 Objective: To compare the therapeutic effects of thoracic endovascular aortic repair (TEVAR) on patients with Stanford B-type aortic dissection (AD) in different stages and the effect on aortic remodeling shape. Methods: A total of 96 patients with Stanford B-type AD treated with TEVAR were enrolled in the study, including 62 patients in the chronic phase (chronic phase group) and 34 patients in the subacute phase (subacute phase group). The clinical data, diameters of true and false lumen before and after operation, complete absorption rate of thrombus in false lumen at the stent segment, aortic remodeling rate, reoperation rate and prognosis were compared between the two groups. Results: The rates of true lumen collapse and tumor-like expansion in the chronic phase group were significantly higher than those in the subacute phase group (P<0.05); and the rate of complete thrombosis was significantly lower than the subacute phase group (P<0.05). The increase in diameter of the true lumen and reduction in diameter of the false lumen in the subacute phase group were significantly greater than those in the chronic phase group (P<0.05). The complete absorption rate of thrombus in false lumen at the stent segment after operation and aortic remodeling rate in the subacute phase group were significantly higher than those in the chronic phase group (P<0.05); and the reoperation rate was significantly lower than the chronic phase group (P<0.05). Follow-up found that the survival rate of the subacute phase group was significantly higher than that of the chronic phase group (χ2=4.810, P=0.028). Conclusion: Compared with chronic Stanford B ad, subacute phase is more stable, and the recovery of true and false lumen, thrombus complete absorption rate and aortic remodeling after TEVAR are better, which can obtain better prognosis. 2020 Vol. 26 (10): 1685-1689 [Abstract] ( 108 ) HTML (1 KB)  PDF (1259 KB)  ( 345 ) 1689 Effects of Stereotactic Targeting Minimally Invasive Surgery on Perioperative Indexes and Prognosis in Patients with Brain Tumors GAO Haifeng, et al DOI: 10.3969/j.issn.1006-6233.2020.10.025 Objective: To observe the effects of stereotactic targeting minimally invasive surgery on perioperative indexes and prognosis in patients with brain tumors. Methods: The clinical data of 152 patients with brain tumors were retrospectively analyzed. According to the treatment procedures, they were divided into stereotactic targeting minimally invasive surgery group (study group, n=84) and conventional craniotomy group (control group, n=68). The operation condition was observed. The patients were followed up for 1 year after operation, and the improvements of neurological function [neurological impairment score (CCD)] and activity of daily life (ADL) were compared between the two groups before and after treatment. Survival rate and recurrence rate at 1 year after operation and occurrence of complications were observed. Results: The operation condition in study group was superior to that in control group (P<0.05). At 1 year after operation, the CCS scores in the two groups were decreased compared with those before operation, and the score in study group was lower than that in control group (P<0.05). The ADL scores were increased compared with those before operation, and the score in study group was higher than that in control group (P<0.05). The survival rate at 1 year after operation in study group was higher than that in control group (P<0.05) while the recurrence rate was lower than that in control group (P<0.05). The incidence rate of complications in study group was lower than that in control group (P<0.05). Conclusions: Compared with traditional craniotomy, stereotactic targeting minimally invasive surgery for brain tumors can improve the operation condition, promote the recovery of neurological function and activity of daily life, improve safety, and effectively improve the prognosis, therefore it is more advantageous. 2020 Vol. 26 (10): 1689-1692 [Abstract] ( 89 ) HTML (1 KB)  PDF (1170 KB)  ( 292 ) 1693 Comparison of Different Dosages of Dexmedetomidine and Propofol in Fiberoptic Bronchoscopy Guided Endotracheal Intubation CONG Lu, WANG Shanjuan DOI: 10.3969/j.issn.1006-6233.2020.10.026 Objective: To explore the application of different dosages of dexmedetomidine and propofol in tracheal intubation guided by fiberbronchoscope. Methods: From January 2017 to January 2019, 80 patients undergoing bronchofiberscope guided endotracheal intubation in our hospital were divided into four groups with 20 cases in each group according to the anesthesia dosage of dexmedetomidine and propofol. The hemodynamic indexes MAP, HR and SpO2 at T0, T1, T2, T3, T4 and T5 were compared between the two groups. Results: Repeated measurement analysis of variance showed that HR, SpO2, MAP time factors, time factors and group interaction were statistically significant (P<0.05); further comparison between the two groups showed that SpO2 value at T2, T3, T4 time was significantly lower than that at to time, map value at T2, T3, T4 time was significantly higher than that at to time; the fluctuation of HR, SpO2, MAP with time in group A was smaller than that in group B, group B was smaller than that in group C, and group C was smaller than that in group D, the difference was statistically significant (P<0.05). The success rate of intubation in group D (70.00%) was significantly lower than that in group A (95.00%) and group C (95.00%), the time of apnea in group C was significantly higher than that in group A, group B, group D, the time of intubation in group C was significantly lower than that in group A, group B, group D (P<0.05). The incidence of respiratory inhibition and bradycardia in group C was significantly higher than that in the other three groups, and the incidence of cough in group D was significantly higher than that in the other three groups (P<0.05). Conclusion: Different dosages of dexmedetomidine and different doses of propofol are safe and effective in fiberoptic bronchoscopy guided endotracheal intubation, and 1.0 μ g / kg dexmedetomidine and 0.8 mg / kg propofol are suitable for stabilizing hemodynamics, improving the success rate of intubation and reducing adverse reactions. 2020 Vol. 26 (10): 1693-1697 [Abstract] ( 80 ) HTML (1 KB)  PDF (1176 KB)  ( 239 ) 1698 Clinical Study on the Correlation between High Positive Rate of HBsAg and Vaccination in Children AN Hebing, MA Yuanyuan, et al DOI: 10.3969/j.issn.1006-6233.2020.10.027 Objective: Through the statistical study on the detection of HBsAg in 0~1 year-old infants within 2 weeks after vaccination, the positive rate was close to 10%. To analyze whether the low titer of HBsAg was positive and the positive rate was relatively high, whether it was related to vaccination, and to explore its mechanism and clinical significance. Methods: Children aged 0~1 years who were vaccinated with hepatitis B vaccine in pediatric ward and immune clinic from 2016 to 2019 were selected. The serum samples of 193 cases with low HBsAg titer positive were detected by chemiluminescent microparticle method within 2 weeks after vaccination. The same detection method was used to retest the HBsAg of these 193 infants aged 0~1 years after half a year, and the positive rate was counted After the vaccine was diluted with different concentrations, the same detection method was used to detect HBsAg, the dilution multiple of positive results was counted, and then the concentration of vaccine in different stages of infants was calculated by intramuscular injection, and the detection results of different types were statistically analyzed. Results: In 193 infants, HBsAg was re-examined half a year later. Four cases were positive, with a positive rate of only 2.1%, indicating that the low titer of HBsAg detected within 2 weeks in 0~1 year old infants with hepatitis b vaccine was directly related to vaccination, and the difference was statistically significant (P<0.05) , HBsAg vaccine in different concentration, test results and vaccines in the different stages of infant concentration analysis of vaccine to dilute concentration in the body should be characterized by negative result, the cause of the high positive rate and low HBsAg drops degrees may be related to stimulate the body to produce the vaccine immune effect, the difference have statistical word meaning (p<0.05). Conclusion: the hepatitis b vaccine inoculation of 0 and 1 years old infants and young children appear HBsAg. Low-grade high positive rate and correlated with vaccination, most of the infants and young children for half a year after testing HBsAg, overcast, there are a few not may be cloudy and vaccination has nothing to do, is once infected with HBV became second liver carrier, through above research and analysis, can provide theoretical basis for the doctor to the clinical diagnosis of whether children are infected with second liver. 2020 Vol. 26 (10): 1698-1701 [Abstract] ( 108 ) HTML (1 KB)  PDF (1168 KB)  ( 301 ) 1702 Analysis on Value of Multivoxel and Monovoxel of 1H-MRS in the Diagnosis of Low-grade Glioma Acute and Subacute Cerebral Infarction and Viral Encephalitis BAI Xuedong, QIU Hengzhi, WANG Shenglin, et al DOI: 10.3969/j.issn.1006-6233.2020.10.028 Objective: To explore the value of H-MRS SVS and CSI in the diagnosis of low-grade gliomas, Acute and subacute cerebral infarction and viral encephalitis. Methods: The clinical and imaging data of 76 patients in our hospital were analyzed retrospectively, including 27 cases of viral encephalitis, 28 cases of cerebral infarction, 21 cases of low-grade gliomas. All patients were examined with magnetic Verio 3.0T MR for routine MR, SVS and CSI, and the spectral data were analyzed with spectroscopy software to compare the ratio of MRS metabolites in the three groups. Results: Cho / Cr, NAA / CHO, NAA / Cr in low-grade gliomas, viral encephalitis and cerebral infarction analysis of variance showed significant differences, further comparisons: There were significant differences in CHO / Cr, NAA / CHO, NAA / Cr between low-grade gliomas and encephalitis (P<0.05), Cho / Cr, NAA / CHO, NAA / CR of low-grade glioma were higher than those of encephalitis group and cerebral infarction group (P<0.05), There was no significant difference in NAA / CHO and NAA / Cr (P>0.05). Lac peak was found in 2 cases of low-grade gliomas, 18 cases of MI peak and 24 cases of cerebral infarction. When Cho / Cr diagnostic threshold was 2.35, the sensitivity and specificity of differentiating low-grade gliomas from viral encephalitis and cerebral infarction were 90.5% and 91% respectively. Conclusion: The combination of multivoxel and single voxel technology of 1 HMRS has certain clinical value in the differential diagnosis of low-grade glioma, acute and subacute cerebral infarction and viral encephalitis. Cho / Cr ratio is of great value in differentiating low-grade glioma from acute and subacute viral encephalitis and cerebral infarction. 2020 Vol. 26 (10): 1702-1706 [Abstract] ( 148 ) HTML (1 KB)  PDF (1584 KB)  ( 740 ) 1706 Application of Dexmetomidine Combined with Cookgas Air-Q Intubated Laryngeal Mask Tracheal Intubation in General Anesthesia Airway Management in Patients with Cervical Spine Injury and Its Effect on Postoperative Cognitive Function AN Haiyan, SUN Yanbin, et al DOI: 10.3969/j.issn.1006-6233.2020.10.029 Objective: To study the value of dexmetomidine combined with Cookgas Air-Q intubated laryngeal mask tracheal intubation in general anesthesia airway management in patients with cervical spine injury and its effect on postoperative cognitive function. Methods: 120 patients with cervical spine injury admitted in our hospital from October 2018 to December 2019 were divided into control group (dexmedetomidine combined with tracheal intubation) and observation group (dexmedetomidine combined with Cookgas Air-Q intubated laryngeal mask tracheal intubation) according to the random digital table method, each with 60 cases. The changes of Ramsay score, heart rate, blood pressure, cognitive function and stress hormone indexes before injecting narcotic drugs (T0), 1 min after injection(T1), immediately after intubation (T2) and 1 minute after intubation (T3) and the occurrence of adverse reactions were compared. Results: Hemodynamic indexes (SpO2, HR, map) were significantly lower in T1 than in T0, and increased in T2 and T3, and the range of hemodynamic indexes in the observation group was lower than that in the control group (P<0.05). Ramsay score increased significantly at T1 than at T0, and then decreased significantly at T2 and T3. Ramsay score of patients in the observation group at T2, T3 was significantly higher than that in the control group (P<0.05). The success rate and tolerance rate of intubation once were significantly higher than those in the control group and the esmolol use rate and agitation rate were significantly lower than those in the control group(P<0.05). The levels of serum adrenal hormone and thyroidene were significantly higher at T2 than at t0 and T1, and then significantly lower at T3, the levels of adrenal hormone and thyroid hormone in the observation group were significantly lower than those in the control group (P<0.05). The score of 1 d MMSE after operation was significantly lower than that of 1 d before operation, and 3d, 5d after operation were significantly increased. The MMSE score of the observation group at 1d, 3d after operation was significantly higher than that of the control group, and the incidence of postoperative cognitive impairment and postoperative pharyngeal pain was significantly lower than that of the control group(P<0.05). Conclusion: Dexmedetomidine combined with cook gas air-Q laryngeal mask airway intubation can effectively stabilize the vital signs and oxidative stress state of patients with cervical spine injury, improve the success rate and tolerance of the first intubation, and improve cognitive function and sedation state. 2020 Vol. 26 (10): 1706-1712 [Abstract] ( 106 ) HTML (1 KB)  PDF (1225 KB)  ( 318 ) 1712 Study on the Correlation between Serum Hcy and NT-pro-BNP and Clinical Prognosis of Patients with Chronic Heart Failure HAI Lan, LEI Xiaoting, WANG Peng DOI: 10.3969/j.issn.1006-6233.2020.10.030 Objective: To explore the correlation between serum homocysteine (Hcy) and N-terminal pro-brain natriuretic peptide (NT-pro-BNP) and clinical prognosis of patients with chronic heart failure (CHF). Methods: 70 cases of CHF patients admitted to department of cardiology of our hospital from January 2017 to March 2019 were included as observation group, and 70 healthy persons who came to the hospital for physical examination during the same period were selected as control group. The ultrasound detection indexes [left ventricular ejection fraction (LVEF), left ventricular end-diastolic diameter (LVEDD)] and serum Hcy and NT-pro-BNP levels were compared between the two groups. Spearman correlation analysis was used to analyze the correlation between serum Hcy and NT-pro-BNP and LVEF and LVEDD. Patients in observation group were treated with anti-heart failure therapy. The occurrence of major adverse cardiovascular events (MACE) were followed up within 1 year after discharge. According to the follow-up results, the patients were divided into MACE group and non-MACE group, and the serum Hcy and NT-pro-BNP levels were compared between the groups. Results: The LVEF of observation group was less than that of control group while the LVEDD and serum Hcy, NT-pro-BNP were significantly higher than those of control group (all P<0.05). Spearman correlation analysis showed that serum Hcy and NT-pro-BNP were negatively correlated with LVEF (r=-0.867,-0.892, all P<0.05), and were positively correlated with LVEDD (r= 0.812, 0.870, all P<0.05). Serum Hcy and NT-pro-BNP of MACE group were significantly higher than those of non-MACE group (all P<0.05). Conclusion: Serum Hcy and NT Pro BNP levels are closely related to cardiac function in patients with CHF, which can provide basis for predicting clinical prognosis. 2020 Vol. 26 (10): 1712-1715 [Abstract] ( 118 ) HTML (1 KB)  PDF (1175 KB)  ( 252 ) 1715 A Study of the Relevance Between lncRNA CCHE1 and Clinical Features Prognosis of Lung Adenocarcinoma TANG Rongbin, WANG Gang DOI: 10.3969/j.issn.1006-6233.2020.10.031 Objective: To explore the relevance between long non-coding RNA (lnc RNA) CCHE1 expression and clinical features, prognosis of lung adenocarcinoma. Methods: Eighty-three patients with lung adenocarcinoma who underwent surgical resection from December 2015 to December 2016 were enrolled. The paraffin blocks of lung cancer tissues and adjacent normal tissues were collected. The expression of lnc RNA CCHE1 was detected by qRT-PCR. The relevance between its expression and clinicopathological features of patients were analyzed. The prognosis of patients was followed up. The value of lnc RNA CCHE1 expression in prognosis determining was analyzed. Results: The expression of lnc RNA CCHE1 in lung adenocarcinoma tissues was significantly higher than that in adjacent tissues (P<0.05). The expression of lnc RNA CCHE1 in patients with high TNM stage, lymph node metastasis and poor differentiation degree was significantly higher than that with low TNM stage, without lymph node metastasis and with high differentiation degree (P<0.05). AUC of lnc RNA CCHE1 for diagnosis of lung adenocarcinoma was 0.925 (95%CI: 0.876-0.974). When lnc RNA CCHE1 was not lower than 29.18, the sensitivity and specificity were 94.0% and 89.2%, respectively. 22 patients died and 61 survived. The expression of lnc RNA CCHE1 in the dead was (73.15 ± 8.45), which was significantly higher than that in the survivors(P<0.05). The 3-year progression-free survival (PFS) rate and overall survival rate in patients with high expression of lnc RNA CCHE1 were 32.84% (22/67) and 68.66% (46/67), which were significantly lower than those with low expression (P<0.05). TNM stage, lymph node metastasis, differentiation degree and lnc RNA CCHE1 level were independent factors affecting PFS and OS in patients with lung adenocarcinoma after 3 years of surgery (P<0.05). Conclusion: The expression of lnc RNA CCHE1 is increased in lung adenocarcinoma tissues. Its expression is relevant to clinicopathological features and prognosis of patients, which can be applied as a potential molecular marker of lung adenocarcinoma. 2020 Vol. 26 (10): 1715-1720 [Abstract] ( 129 ) HTML (1 KB)  PDF (1394 KB)  ( 360 ) 1720 A Value Assessment of NT ProBNP, CTnI and D-D Levels in the Risk of Acute Pulmonary Embolism WANG Qingsong, WANG Zhuxiu DOI: 10.3969/j.issn.1006-6233.2020.10.032 Objective: To investigate the value of plasma N-terminal B-type natriuretic peptide (NT proBNP), Troponin (cTnI), D-Dimer (D-D) levels in the risk of acute pulmonary embolism (APE). Methods: The clinical data of 120 patients with high-risk APE admitted to the hospital from January 2017 to January 2019 were analyzed retrospectively. The proportion of APE patients was statistically analyzed. The levels of NT proBNP, cTnI and D-D were compared between the occurrence group and the non-occurrence group. The plasma NT-proBNP, cTnI, and D-D levels were analyzed to evaluate whether APE occurred in high-risk APE. Results: The patients who got APT accounted for 22.88%, and the levels of NT proBNP, cTnI and D-D in the occurrence group were higher than that in the non-occurrence group (P<0.05). Plasma NT-proBNP, cTnI, D-D levels had the best cutoff points to assess whether APE occurs, which were 1987.38pg/mL, 75.41μg/mL and 5.86mg/L, and the specificity and AUC of the combination of NT proBNP, cTnI and D-D were the highest. There were significant differences between the AUC of NT proBNP, cTnI and D-D level combination assessment and those of single assessment (P<0.05). Conclusion: The levels of NT proBNP, cTnI and D-D in the plasma of APE patients are all increased, and the combination of the levels of NT proBNP, cTnI and D-D is ideal to the evaluation of the occurrence of APE. 2020 Vol. 26 (10): 1720-1723 [Abstract] ( 92 ) HTML (1 KB)  PDF (1307 KB)  ( 258 ) 1723 Clinical Observation of 5-FU Sustained-release Seeds Implantation Combined with Tegafur in the treatment of Digestive Tract Tumor LIU Xianxing, LIANG Jingbo, CHANG Fan, et al DOI: 10.3969/j.issn.1006-6233.2020.10.033 Objective: To observe the curative effect of endoscopic implantation of slow-releasing 5-fluorour acil in combination with tegafur on gastrointestinal tumors. Methods: From March 2011 to March 2014, 70 patients with gastrointestinal tumors were treated in our hospital. These enrolled patients were simple randomly divided into observation group and control group according to the random principles, with 35 cases in each group. Both groups were treated with tegafur, and the observation group was treated with 5-fluorour acil on the basis of control group. The efficacy and survival quality of the two groups were compared after 3 months. Results: After 3 months' treatment, the observation group effective rate was higher (P<0.05). when survival quality efficiency rate and total efficiency rate were comapared, the observation group was higher than the control group respectively (P＜0.05). After 3 months of treatment, the fatigue status of the two groups was compared. The observation group was slightly less fatigued and had lower fatigue score than control group(P<0.05). Conclusion: Curative therapy of endoscopic implantation of slow-releasing 5-fluorour acil in combination with tegafur is effective and can improve the survival quality for patients with gastrointestinal tumors. Thus, it's well worth of being popularized. 2020 Vol. 26 (10): 1723-1727 [Abstract] ( 82 ) HTML (1 KB)  PDF (1480 KB)  ( 233 ) 1727 The Establishment and Clinical Value of Medians of Serum Markers in Down's Syndrome Prenatal Screening MI Dongqing, SONG Ge, PENG Yuanyuan, et al DOI: 10.3969/j.issn.1006-6233.2020.10.034 Objective: To establish the median equation of serum markers in prenatal screening in the early and middle pregnancy in Shijiazhuang area and explore the screening efficiency and clinical value of the localized median equation. Methods: We collected the screening data of the 28930 pregnant women in the first and second trimester from January 2017 to November 2018. The median values of PAPP-A, free hCGβ, AFP, uE3 and NT in both the software record and in local population were compared. Then the data were applied to the evaluation of the risk of Down's syndrome and compared the difference of the screening efficiency. Results: 1. The median concentrations of PAPP-A, free hCGβ, uE3 in Shijiazhuang were higher than those of Caucasian population. The difference was statistically significant(P<0.05). There was no significant difference in the median concentration of AFP between the local population and the Caucasian population(P>0.05). 2. There were significant differences between the MoM median of serum markers of local population and software median equation (P<0.05). 3. By using the median of local population, the high-risk rate of trisomy 21 was 2.33%. The detection rate of trisomy 21 was 94.59%. By using the software's built-in median, the high-risk rate of trisomy 21 was 2.16%. The detection rate of trisomy 21 was 86.48%. The difference was statistically significant(P<0.05). Conclusion: The medians of serum markers were significantly different between the population of Shijiazhuang and the Caucasus population. The application of the localized median can significantly improve the screening efficiency. For the development of Down's screening in Shijiazhuang, it is of great significance to establish the localized median database of serums markers in the first and second trimester of pregnancy. 2020 Vol. 26 (10): 1727-1733 [Abstract] ( 122 ) HTML (1 KB)  PDF (1914 KB)  ( 300 ) 1733 To Investigate the Value of Noninvasive Prenatal DNA (NIPT) in Diagnosing Abnormal of Abnormal Karyotype in Elderly Pregnant Women MA Zhihui DOI: 10.3969/j.issn.1006-6233.2020.10.035 Objective: To explore the value of noninvasive prenatal DNA (NIPT) in the diagnosis of abnormal karyotype in elderly pregnant women. Methods: 1467 elderly pregnant women who were admitted to our hospital from January 2016 to January 2019 and voluntarily received NIPT test were selected as subjects. Peripheral blood samples of all subjects were collected and free DNA of fetuses was extracted. After PCR amplification, NIPT detection was used to calculate the risk of chromosomal diseases in children. Meanwhile, amniocentesis tests were performed on pregnant women with high-risk NIPT test results. The abnormal NIPT test results and the verification results of amniotic fluid karyotype in 1467 pregnant women were compared, and the coincidence rate of the abnormal NIPT test results and the verification results of amniotic fluid karyotype were analyzed. To compare the detection rate of abnormal karyotype in pregnant women of different ages. Results: There were 24 cases of 1467 elderly pregnant women with abnormal NIPT detection, among which 6 cases indicated high risk of trisomy 21, 4 cases indicated high risk of trisomy 18, 3 cases indicated high risk of trisomy 13, 7 cases indicated sex chromosome abnormalities, and 4 cases indicated other chromosome abnormalities. Amniocentesis test was performed in 24 pregnant women, and 13 cases of fetal chromosomal abnormalities were diagnosed, including 6 cases of trisomy 21, 3 cases of trisomy 18, 1 case of trisomy 13, 2 cases of sex chromosome abnormalities and 1 case of other chromosomal abnormalities. The remaining 11 pregnant women with normal nuclear type were confirmed by amniotic fluid. The coincidence rate of NIPT detection with the verification result of amniotic fluid karyotype was 54.17%, among which the coincidence rate of NIPT detection with the verification result of amniotic fluid karyotype was 100.00%, 75.00%, 33.33%, 28.57% and 25.00%, respectively. The detection rate of abnormal karyotype in elderly pregnant women aged ≥40 years was 1.82% (9/494), which was significantly higher than 0.41% (4/973) in elderly pregnant women aged 35-39 years (P<0.05).. Conclusion: The value of NIPT detection in elderly pregnant women is fair, and the detection rate of trisomy 21 and trisomy 18 is high. Which is worthy of clinical application. With the increase of the age of pregnant women, the higher the detection rate of abnormal nuclear type of NIPT, which should be paid attention to. 2020 Vol. 26 (10): 1733-1738 [Abstract] ( 95 ) HTML (1 KB)  PDF (1199 KB)  ( 303 ) 1738 An Analysis of the Relevance Between Treg/Th17 Cell Imbalance, Expression Levels of Related Cytokines and Aplastic Anemia WANG Rui, DONG Caili, ZHAO Yunting, et al DOI: 10.3969/j.issn.1006-6233.2020.10.036 Objective: To explore the relevance between Treg/Th17 cell imbalance, expression levels of related cytokines and aplastic anemia (AA). Methods: 94 AA patients who were admitted to the cooperative hospital from December 2016 to June 2018 were enrolled. According to disease severity, they were categorized into mild AA (MAA) group (41 cases) and severe AA (severe AA) group (53 cases). 50 healthy people who underwent physical examination in the hospital during the same period were enrolled as control group. The levels of Treg and Th17 in peripheral blood were detected by flow cytometry. The levels of serum IL-10, IL-6 and IL-17 were detected by enzyme-linked immunosorbent assay (ELISA). The relevance between Treg/Th17 cell imbalance, related cytokines and AA severity, prognosis was analyzed. Results: Treg cell level and Treg/Th17 in SAA group were lower than those in control group and MAA group, while Th17 level was higher than that in control group and MAA group (P<0.05). The level of IL-10 in SAA group was lower than that in control group and MAA group, while levels of IL-6 and IL-17 were higher than those in control group and MAA group (P<0.05). The levels of Treg, Treg/Th17 and IL-10 were negatively correlated with disease severity (r1=-0.604, r2=-0.661, r3=-0.611, P<0.05). Th17, IL-6 and IL-17 were positively correlated with disease severity (r1=0.475, r2=0.486, r3=0.535, P<0.05). After multivariate Logitic regression model analysis, Treg and Treg/Th17 were protective factors influencing disease prognosis (acute recurrence or death) (P<0.05). Conclusion: There is an imbalance of Treg/Th17 cells and related cytokines in AA patients. The imbalance of Treg/Th17 cells is closely related to the severity and prognosis of AA. 2020 Vol. 26 (10): 1738-1742 [Abstract] ( 77 ) HTML (1 KB)  PDF (1193 KB)  ( 278 ) 1742 The Clinical Efficacy of Anlotinib Hydrochloride in the Treatment of Advanced Non-Small Cell Lung Cancer Patients ZHANG Yu, LI Yanfang, ZHANG Gaiqin, et al DOI: 10.3969/j.issn.1006-6233.2020.10.037 Objective: To observe the quality of life and clinical efficacy on anlotinib hydrochloride in the treatment of advanced non-small cell lung cancer patients. Methods: 37 cases who were diagnosed with non-small cell lung cancer were collected from our hospital;anlotinib hydrochloride was administered to all patients, once a day, 12 mg a time, before breakfast, then the clinical efficacy, effect on patients' quality of life, and adverse reactions of anlotinib hydrochloride in the treatment of this disease were analyzed. Results: The objective remission rate was 35.1%, while the disease control rate of anlotinib hydrochloride was 89.2%. After treatment, the scores of daily life, emotional control, activity, social/family life and other scores of patients were higher than before treatment, and the total score (181.39±14.73) after treatment was higher than before treatment (160.73±12.16). The differences were statistically significant (P<0.05). Anlotinib hydrochloride had the highest incidence of hypertension in patients with non-small cell lung cancer after treatment, accounting for 13.5%, followed by hand-foot syndrome and thyroid dysfunction, accounting for 10.8% and 8.1%, respectively. Six of the patients had serious adverse reactions, accounting for 8.1%. Conclusion: Anlotinib has certain advantages in the efficacy and safety of treatment of non-small cell lung cancer, and can improve the quality of life of patients, and it can provide treatment for patients with second-and third-line chemotherapy failure and the refractory. 2020 Vol. 26 (10): 1742-1746 [Abstract] ( 95 ) HTML (1 KB)  PDF (1190 KB)  ( 255 ) 1746 Effect of Xiaoer Chaigui Tuire Granule on Physical Signs and Immune Function in the Treatment of Acute Upper Respiratory Tract Infection ZHAO Xin, HE Yan-yao, TIAN Jing DOI: 10.3969/j.issn.1006-6233.2020.10.038 Objective: To investigate the effect of Xiaoer Chaigui Tuire granule on physical signs and immune function in the treatment of acute upper respiratory tract infection. Methods: 228 children with acute upper respiratory tract infection in our hospital from January 2013 to December 2017 were selected, and divided into two groups according to random number method, each with 114 cases. Observation group received Xiaoer Chaigui Tuire granule and ribavirin injection, while control group received routine treatment. Temperature at admission, 24h, 48h and 72h were recorded and compared between groups. Then the clinical efficacy and immune function were analyzed. Results: The total effective rate was significantly higher in observation group than in control group (97.37% vs 84.21%, P<0.05). At 24h, 48h and 72h after admission, body temperature showed a decrease in both groups (P<0.05), and was lower in observation group than in control group (P<0.05). After treatment, there was an increase in CD3+, CD4+ and CD4+/CD8+ levels, and a decrease in CD8+ level in both groups (P<0.05), and observation group had significantly higher CD3+, CD4+ and CD4+/CD8+ levels, and lower CD8+ level than those of control group after treatment (P<0.05). CRP and WBC were decreased in both groups after treatment (P<0.05), and were lower in observation group than in control group (P<0.05). The clinical effect of observation group was positively correlated with immune function indicators CD3+, CD4+, CD8+, and CD4+/CD8+. Conclusion: Application of Xiaoer Chaigui Tuire granule as an adjuvant treatment for children with acute upper respiratory tract infection can effectively enhance the immune function and rapidly reduce the body temperature. 2020 Vol. 26 (10): 1746-1750 [Abstract] ( 157 ) HTML (1 KB)  PDF (1187 KB)  ( 298 ) 1750 Curative Efficacy of Nicorandil on Acute Coronary Syndrome and Its Effects on Coronary Microcirculation and Serum Inflammation Indexes WANG Yahong, XIAO Hongyan, PENG Qi DOI: 10.3969/j.issn.1006-6233.2020.10.039 Objective: To analyze the effects of nicorandil on TIMI classification, coronary microcirculation, and serum inflammation indexes after CABG (coronary artery bypass surgery) in patients with ACS (acute coronary syndrome). Methods: 86 patients with ACS admitted to our hospital from January 2018 to December 2019 were grouped with random number table (43 cases in each group). The basic treatment group was given routine clinical treatment after CABG surgery, and the nicorandil group was treated with nicorandil more than the treatment group, and the TIMI blood flow classification, coronary microcirculation, serum inflammation indicators, and adverse reactions were compared between the two groups. Results: After 4 weeks of treatment, the TIMI grade of the nicorandil group was significantly better than that of the basic treatment group. The IMR, IL-6, hs-CRP, and TNF-a indexes of the nicorandil group were significantly lower than those of the basic treatment group. The differences were statistically significant (P<0.05). The incidence of adverse reactions (9.30%) in the nicorandil group was compared with the basic treatment group (6.98%), and the difference was not statistically significant (P>0.05). Conclusion: Nicorandil applied to patients with ACS after CABG surgery can effectively improve coronary reperfusion, reduce inflammation, and has fewer adverse reactions, which is worthy of reference. 2020 Vol. 26 (10): 1750-1754 [Abstract] ( 109 ) HTML (1 KB)  PDF (1187 KB)  ( 251 ) 1754 Curative Effect of Sulperazone Combined with Ambroxol on Neonatal Infectious Pneumonia and Their Influences on Immunity SU Lina, LI Wenlin, CHEN Caihua, et al DOI: 10.3969/j.issn.1006-6233.2020.10.040 Objective: To observe the clinical curative effect of sulperazone combined with ambroxol hydrochloride on neonatal infectious pneumonia, and to explore their influences on respiratory flora and immune status. Methods: The clinical case data of 98 neonates with infectious pneumonia who were admitted to the hospital from October 2017 to October 2019 were retrospectively analyzed. According to different medication methods, they were divided into control group (n=46) and combination group (n=52). The control group was given sulperazone for anti-infective treatment on basis of routine oxygen inhalation and nutritional support, while combination group was given ambroxol hydrochloride for aerosol inhalation on basis of control group. The clinical curative effect, changes in arterial oxygen partial pressure (PaO2) and arterial partial pressure of carbon dioxide (PaCO2), disappearance time of clinical symptoms, length of hospital stay, immunoglobulin (IG) level and adverse reactions were compared between the two groups. Results: The total response rate in combination group was higher than that in control group (P<0.05). After treatment, level of PaO2 was increased in both groups, and the combination group had larger rangeability than control group (P<0.05). After treatment, level of PaCO2 was decreased in both groups, and the combination group had larger rangeability than control group (P<0.05). The disappearance time of asthma and lung signs in combination group were earlier than that in control group, and hospital stay was significantly shorter than that in control group (P<0.05). After treatment, levels of IgM, IgA, and IgG in combination group were higher than those in control group (P<0.05). There was no significant difference in total incidence of adverse reactions between the two groups (P>0.05). Conclusion: The curative effect of sulperazone combined with ambroxol hydrochloride for aerosol inhalation neonatal is significant on neonatal pneumonia. It can effectively improve arterial blood gas, promote disappearance of clinical symptoms, and shorten the course of disease. 2020 Vol. 26 (10): 1754-1758 [Abstract] ( 116 ) HTML (1 KB)  PDF (1188 KB)  ( 655 ) 1758 Analysis of Preventive Detection Results of 731 Cases of Fetal and Neonatal Hemolytic Disease MA Ling DOI: 10.3969/j.issn.1006-6233.2020.10.041 Objective: Detection of irregular antibody and its titer in serum of pregnant women,To assess the risk of fetal and neonatal hemolytic disease due to maternal-infant Blood Group incompatibility.Methods: from 2015 to 2019, blood samples from 731 pregnant women with hemolytic disease of the newborn were collected, Abo and RH blood group systems are tested for blood group, and antibody titers are measured by indirect antiglobulin assay in test tubes, Objective: to analyze the correlation between the detection rate of Abo Blood Group System Antibody titer ≥64 and RH blood group system titer ≥32 and the incidence rate of neonatal diseases.Results: of the 731 blood samples from pregnant women and newborns suspected of neonatal hemolytic disease, The detection rate of ABO-hdfn (+) in Abo blood group system was 11.36% (83/731) , The detection rate of Rh-HDFN (+) in RH blood group system was 2.06% (15/731) , There were statistical differences (p < 0.05);Of the 98 high-risk HDFN pregnant women, 74 cases of hemolytic disease of the newborn, The incidence of HDFN was 75.51% (74/98) , and the higher the antibody titer, the higher the risk of HDFN, The prevalence of HDFN was highest when the titer of antibody was ≥1:128, The positive rate of 0 ~ 3 days after birth was significantly higher than 4 ~ 10 days after birth, There was significant difference (88.71% vs 52.78% p < 0.05).Conclusion: The detection rate of Abo Blood Group system is higher than that of RH blood group system, The highest number and detection rate of type O were found in pregnant women, and the prevalence rate was proportional to the value of antibody, The positive rate was higher when the samples were sent within 3 days after birth. 2020 Vol. 26 (10): 1758-1760 [Abstract] ( 93 ) HTML (1 KB)  PDF (1149 KB)  ( 257 )

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