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2020 Vol. 26, No. 8 Published: 2020-08-31 1233 Research on MiR-1231 Inhibiting Meningioma Cell Proliferation by Targeting EGFR/PI3K/AKT Pathway CHEN Bo, GUO Yutao, et al DOI: 10.3969/j.issn.1006-6233.2020.08.001 Objective: To investigate the effect of miR-1231 on meningioma cell proliferation by targeting the EGFR /PI3K /AKT pathway. Methods: Bioinformatics software and luciferase reporter gene experiments were used to analyze and verify the targeted binding between miR-1231 and EGFR. RT-PCR was used to detect miR-1231 expression and EGFR mRNA expression in meningiomas cells. Western blot was used to detect EGFR protein expression, PI3K and AKT phosphorylation levels; CCK-8 was used to detect meningioma cell viability; colony formation assay and EdU assay was used to detect meningioma cell proliferation. Results: Compared with normal meningeal cells, the expression of miR-1231 in meningiomas cells was significantly down-regulated (P<0.05), and the expression of EGFR was significantly up-regulated (P<0.05). EGFR was confirmed as a target gene of miR-1231. Compared with miR-NC group, miR-1231 significantly inhibited meningiomas cell proliferation and inhibited EGFR/PI3K/AKT pathway (P<0.05). Compared with anti-miR-NC group, anti- miR-1231 significantly promotes meningioma cell proliferation and activates the EGFR /PI3K /AKT pathway (P<0.05). Compared with miR-NC + EGFR-NC group, miR-1231 + EGFR-NC significantly inhibits meningiomas cells EGFR /PI3K /AKT pathway (P<0.05), significantly reduced cell proliferation (P<0.05). Compared with miR-NC + EGFR-NC group, miR-NC + EGFR significantly activated meningiomas cells PI3K /AKT pathway (P<0.05) ), significantly increased cell proliferation ability (P<0.05), and could significantly reverse the inhibitory effects of miR-1231 + EGFR-NC on PI3K /AKT pathway and cell proliferation ability of meningiomas cells. Conclusion: MiR-1231 can inhibit the proliferation of meningioma cells by targeting the EGFR to regulate the PI3K /AKT pathway. 2020 Vol. 26 (8): 1233-1236 [Abstract] ( 207 ) HTML (1 KB)  PDF (1993 KB)  ( 367 ) 1237 Effects of Razumab on Serum VE-Cadherin and bFGF in Diabetic Retinopathy Patients Before and After Operation SUN Wenjuan, JI Fengtao, LI Yongrong DOI: 10.3969/j.issn.1006-6233.2020.08.002 Objective: To investigate the effects of razumab on serum vascular endothelial cadherin (VE-cadherin) and basic fibroblast growth factor (bFGF) in patients with diabetic retinopathy during vitrectomy. Methods: 85 patients with diabetic retinopathy admitted to our hospital from March 2018 to May 2019 were randomly divided into two groups, 43 cases in study group and 42 cases in control group. The control group received vitrectomy, while the study group received intravitreal injection of razumab on the basis of the control group. The operation condition, visual acuity improvement and levels of serum vascular endothelial growth factor (VEGF), bFGF and VE-cadherin were observed in the two groups, and the complications were evaluated. Results: The operation time and intraoperative bleeding volume in the study group were significantly less than those in the control group (P<0.05). The macular fovea retinal thickness was significantly smaller than that in the control group (P<0.05). The visual acuity improvement rate was significantly higher than that of the control group (83.72 % VS 57.14 %, P<0.05). After treatment, the levels of serum VEGF, bFGF and VE-cadherin in the two groups were lower than those before treatment (P<0.05), and the levels of serum VEGF, bFGF and VE-cadherin in the study group were significantly lower than those in the control group (P<0.05). In addition, the incidence of postoperative complications in the study group was 6.98%, which was significantly lower than 23.81% in the control group (P<0.05). Conclusion: The intravitreal injection of rapunzumab before operation can optimize the operation environment, reduce the risk of operation, effectively improve vision and reduce postoperative complications. The clinical effect is significant and worthy of promotion. 2020 Vol. 26 (8): 1237-1240 [Abstract] ( 106 ) HTML (1 KB)  PDF (1200 KB)  ( 296 ) 1241 Effect of Ultrasound-guided Lauryl Alcohol Assisted Radiofrequency Ablation on Blood Flow Classification Pain and Sex Hormone Levels in Patients with Uterine Leiomyoma LI Huimin, HUANG Beibei DOI: 10.3969/j.issn.1006-6233.2020.08.003 Objective: To investigate the effects of blood flow grading, pain improvement and serum sex hormone levels on uterine leiomyoma patients undergoing radiofrequency ablation guided by transabdominal ultrasound. Methods: From January 2017 to May 2018, 58 patients undergoing radiofrequency ablation were divided into study group (30 cases) and control group (28 cases) by random number table method. The patients with uterine myoma in the control group were treated with radiofrequency ablation guided by transabdominal ultrasound. The changes of sex hormones in blood flow, pain score (VAS) and follicle-forming hormone (FSH), luteinizing hormone (LH) and serum estradiol (E2) were compared before and after 3 months of treatment. Results: There was no significant difference in blood flow distribution, VAS score and serum sex hormone content between the two groups before ablation(P>0.05). After 3 months of ablation, the blood flow grade of the two groups decreased significantly(P<0.05). The improvement effect of VAS was better than that of control group(P<0.05).There was no significant difference in the concentrations of FSH, LH and E2 between the two groups after ablation(P>0.05). Conclusion: For patients with uterine leiomyoma with transabdominal ultrasound guided radiofrequency ablation using polycoylenol adjuvant therapy, the blood flow grading, pain relief can be more significantly improved, and do not affect the level of sex hormone in patients, the clinical application value is high. 2020 Vol. 26 (8): 1241-1245 [Abstract] ( 159 ) HTML (1 KB)  PDF (1210 KB)  ( 297 ) 1246 The Effect of Bone Cement-Reinforced Pedicle Screw Fixation in the Treatment of Osteoporosis with Disc Degenerative Disease and Its Influencing Factors WANG Ke, et al DOI: 10.3969/j.issn.1006-6233.2020.08.004 Objective: To observe the effect of bone cement-reinforced pedicle screw (PMMA-PS) in the treatment of osteoporosis (OP) with disc degenerative disease (DDD) and related factors. Methods: The data of 80 cases of OP with DDD in our hospital from August 2014 to June 2019 were analyzed retrospectively. According to the different therapeutic methods, the patients were divided into PMMA group (n = 37, with PMMA-PS internal fixation treatment) and control group (n = 43, with regular PS internal fixation treatment).The clinical data, operation time, intraoperative blood loss and screw loosening rate were recorded. Before and 3 months after operation, the height of adjacent intervertebral space, the Cobb angle of fixed segment and the visual simulation score of pain (VAS), were observed, as well as the low back pain score of the Japanese Orthopaedic Association (JOA), and the Oswestry Disability Index (ODI). Further, single factor and logistic multiple regression analysis are adopted to analyze the risk factors that affect the recovery effect. Results: Compared with pre-operation date and data of the control group, the height of adjacent intervertebral space, Cobb angle, VAS score, JOA score and ODI index of fixed segment in PMMA group were significantly improved 3 months after operation (P all<0.05). The single factor analysis showed that there was a significant difference in the score of age, bone density, operation, preoperative VAS and pre-operative JOA(P all<0.05).The multiple logistic regression analysis showed that the preoperative VAS score, bone density and operation were the independent factors of the recovery in PS (P all<0.05). Conclusion: Compared with simple PS internal fixation, PMMA-PS internal fixation can effectively improve low back and leg pain in patients with OP with DDD and promote postoperative functional recovery. PMMA-PS internal fixation has certain clinical application value. And the pre-operative VAS score, bone density, and the operation mode can influence the recovery effect, and the prognosis of the patient can be improved by enhancing the bone strengthening mode such as OP treatment and the like. 2020 Vol. 26 (8): 1246-1250 [Abstract] ( 94 ) HTML (1 KB)  PDF (1198 KB)  ( 235 ) 1251 Comparative Analysis of Sputum Acid Fast Bacilli Smear and Gene Xpert MTB in Adult Pulmonary Tuberculosis Patients with and without T2DM HE Haini, et al DOI: 10.3969/j.issn.1006-6233.2020.08.005 Objective: To study the difference of sputum acid fast bacilli smear and gene xpert MTB detection between adult pulmonary tuberculosis patients with and without T2DM. Methods: 43 patients with tuberculosis with T2DM and 43 patients without T2DM who were admitted to our hospital from January 2019 to December 2019 were studied to evaluate the difference between the two groups of patients with T2DM smear and Gene Xpert MTB. Results: The incidence of hemoptysis in tuberculosis group with T2DM was significantly higher than that without T2DM. The sensitivity of two methods in tuberculosis combined with T2DM group had no significant difference, P>0.05. The diagnostic sensitivity of MTB method was significantly higher than that of acid fast bacilli smear method in pulmonary tuberculosis without T2DM group (P<0.05). There was no significant difference between the two methods in the diagnosis of pulmonary tuberculosis complicated with T2DM, P>0.05. There was no statistical significance between the two detection methods in pulmonary tuberculosis combined with T2DM group (P>0.05). There was statistical significance between the two detection methods of acid fast bacilli smear and gene xpert MTB in the pulmonary tuberculosis without T2DM group, P<0.05. Conclusion: The diagnostic effect of the two methods is similar when pulmonary tuberculosis complicated with T2DM. The sensitivity of gene xpert MTB is better in patients without T2DM. 2020 Vol. 26 (8): 1251-1254 [Abstract] ( 100 ) HTML (1 KB)  PDF (1188 KB)  ( 287 ) 1255 Relationship between Levels of CRP cTnT TSLP miR-30c-5p and Severity of Coronary Artery Disease in Patients with Stable Angina Pectoris HUANG Yubing, LIAO Wang, WANG Miao, et al DOI: 10.3969/j.issn.1006-6233.2020.08.006 Objective: To investigate the relationship between the levels of C-reactive protein (CRP), serum troponin T (cTnT), thymic stromal lymphopoietin (TSLP), miR-30c-5p and the severity of coronary artery disease in patients with stable angina pectoris (SAP). Methods: The clinical data of 90 SAP patients confirmed by coronary angiography (CAG) admitted to the hospital from May 2016 to May 2019 were retrospectively analyzed. They were divided into mild groups according to SYNTAX score (0~22 points, 41 cases). , Moderate group (23~32 points, 32 cases), Severe group (≥33 points, 17 cases). Collect clinical data of three groups of patients, collect blood to measure the CRP, cTnT, TSLP, miR-30c-5p levels of the three groups of patients. The severity of coronary artery disease in the three groups of patients was evaluated by SYNTAX score, and CRP, cTnT, Relationship between TSLP, miR-30c-5p levels and SYNTAX score. Results: The CRP, cTnT, TSLP, miR-30c-5p levels, and SYNTAX scores of the three groups of patients were statistically significant (P all<0.05). Pairwise comparisons of LSD-t tests showed statistically significant differences (P all<0.05). Pearson correlation analysis showed that CRP, cTnT, TSLP and SYNTAX scores were significantly positively correlated (P<0.01), while miR-30c-5p was significantly negatively correlated with SYNTAX scores (P<0.01). Conclusion: CRP, cTnT, TSLP, miR-30c-5p levels correlate with the severity of coronary artery disease in patients with stable angina pectoris. Early detection of the above biological markers can help determine the severity of coronary artery disease in patients with stable angina pectoris. 2020 Vol. 26 (8): 1255-1259 [Abstract] ( 136 ) HTML (1 KB)  PDF (1200 KB)  ( 281 ) 1260 Analysis on the Relationship between the Changes of Serum Hs-CRP ESR and Prognosis in Patients with Active Tuberculosis FENG Xiong, ZHENG Youbin DOI: 10.3969/j.issn.1006-6233.2020.08.007 Objective: To investigate the changes of serum high-sensitivity C-reactive protein (hs CRP) and ESR in patients with active pulmonary tuberculosis and their relationship with prognosis. Methods: The clinical data of 79 patients with active pulmonary tuberculosis treated in our hospital from May 2018 to may 2019 were retrospectively analyzed. According to the sputum smear and sputum culture results after 4 months of anti tuberculosis treatment, they were divided into negative group (62 cases) and positive group (17 cases). The levels of hs-CRP and ESR in the two groups were detected, and the relationship between the levels of hs-CRP and ESR and the prognosis of patients with active pulmonary tuberculosis was analyzed by multivariate logistic regression. Results: There was no significant difference between the two groups (P>0.05). Before treatment, there was no significant difference in hs-CRP and ESR between the two groups (Pall>0.05). After treatment, the levels of hs-CRP and ESR in the two groups were lower than those before treatment, the difference was statistically significant (P<0.05), and the levels of hs-CRP and ESR in the negative group were lower than those in the positive group, the difference was statistically significant (P<0.05). Multivariate logistic regression analysis showed that serum hs-CRP and ESR levels were independent risk factors affecting the prognosis of patients with active tuberculosis (P<0.01). Conclusions:The changes of serum high-sensitivity C-reactive protein and ESR have a certain relationship with the prognosis of patients with active pulmonary tuberculosis. Dynamic monitoring of the changes of serum high-sensitivity C-reactive protein and ESR has a guiding significance for evaluating the prognosis of patients with active pulmonary tuberculosis. 2020 Vol. 26 (8): 1260-1263 [Abstract] ( 252 ) HTML (1 KB)  PDF (1189 KB)  ( 714 ) 1264 Effects of Madopar Combined with Pramipexole on Plasma MicroRNA -124, MicroRNA -137 and Non-Motor Symptoms in Patients with Parkinson's Disease WANG Jing, ZHANG Zhuo, LI Xue DOI: 10.3969/j.issn.1006-6233.2020.08.008 Objective: To study the effects of madopar combined with pramipexole on plasma microRNA -124(miR-124), microRNA -137(miR-137) and non-motor symptoms in patients with Parkinson's disease. Methods: 82 patients with Parkinson's disease who were treated in our hospital from July 2017 to April 2019 were randomly divided into control group and research group, 41 cases in each. The control group was treated with madopar, The research group was treated with madopar and pramipexole. The clinical effects of the two groups were observed and compared. The Parkinson's disease score scale (UPDRS), plasma miR-124 and miR-137 levels, improvement of non-motor symptoms and drug side effects were recorded and compared. Results: 3 months after treatment, the total effective rate of the research group was higher than that of the control group (P<0.05). One month and three months after treatment, the UPDRS scores of the two groups decreased, and the research group was lower than the control group. The UPDRS scores of the two groups were statistically different concerning different treatment methods and time (P<0.05). One month and three months after treatment, the plasma miR-124 in the two groups increased, and the research group was higher than the control group. the plasma miR-137 in the two groups decreased, and the research group was lower than the control group. the plasma miR-124 and miR-137 in the two groups were statistically different in different treatment methods and time (P<0.05). The rates of depression, anxiety and insomnia in the study group were lower than those in the control group (P<0.05). there was no statistical difference in the incidence rates of cognitive impairment, somnolence, constipation and hypotension between the two groups (P>0.05). There was no statistical difference in the total incidence of side effects between the two groups (P>0.05). Conclusion: Madopar combined with pramipexole can improve the curative effect of Parkinson's disease, regulate the expression of miR-124 and miR-137 in plasma, and improve non-motor symptoms. 2020 Vol. 26 (8): 1264-1268 [Abstract] ( 117 ) HTML (1 KB)  PDF (1211 KB)  ( 291 ) 1269 Predictive Value of Serum HAP IL-6 on the Hemorrhage Volume in Patients with Aneurysmal Subarachnoid Hemorrhage CAO Bing, DING Qi, LIU Chunjiang, et al DOI: 10.3969/j.issn.1006-6233.2020.08.009 Objective: To explore the predictive value of serum heparanase (HPA), interleukin (IL) -6 on the hemorrhage volume in patients with aneurysmal subarachnoid hemorrhage (aSAH) and its risk factors, so as to provide theoretical basis for clinical diagnosis and treatment. Methods: From June 2015 to June 2019, 219 patients with aSAH were selected as the study subjects. According to glasgow outcome scale (GOS), the patients were divided into poor prognosis group (85 cases) and good prognosis group (134 cases). The serum levels of HPA, IL-6 and hs-CRP were measured by ELISA. Univariate analysis and multivariate logistic regression were used to analyze the risk factors of prognosis in patients with aSAH. The predictive value of serum HPA, IL-6 and hs-CRP on prognosis in patients with aSAH were evaluated by ROC curve. Results: The serum levels of HAL, IL-6 and hs-CRP in patients with poor prognosis group were higher than those of in patients with good prognosis group (P<0.01). The area under ROC curve of HPA, IL-6 and hs-CRP were 0.846 (95% CI:0.791-0.891), 0.818 (95% CI:0.761-0.867) and 0.732 (95% CI:0.668-0.790) respectively in predicting large hemorrhage volume in patients with Asah, and the prediction effect of HAP and IL-6 were better than hs-CRP. The multivariate logistic regression analysis showed that the age over 65 years old (OR=3.365), history of hypertension (OR=1.792), GCS score <12 points (OR=7.699), HPA≥3.91 ng/mL (OR=1.988), IL-6≥47.34 pg/mL (OR=2.195) and hs-CRP≥7.87 mg/L (OR=1.784) were the risk factors of prognosis in patients with aSAH. Conclusion: Serum levels of HPA, IL-6 and hs-CRP are closely related to the prognosis of aSAH patients. They could be used as indicators for predicting the prognosis in patients with aSAH, but the predictive value of HPA and IL-6 are better than those of hs-CRP. 2020 Vol. 26 (8): 1269-1272 [Abstract] ( 118 ) HTML (1 KB)  PDF (1345 KB)  ( 299 ) 1273 Regulatory Effect of Comprehensive Treatment with Blue Light Irradiation on Serum Indexes of Neonatal Hemolysis with Hyperbilirubinemia LI Kequan, et al DOI: 10.3969/j.issn.1006-6233.2020.08.010 Objective: To study the regulatory effect of blue light irradiation combined with comprehensive treatment on serum indexes of hyperbilirubinemia caused by hemolytic disease of the newborn. Methods: 90 children with hyperbilirubinemia caused by hemolytic disease of the newborn were randomly divided into two groups: the intervention group was treated with blue light irradiation combined with comprehensive drug treatment; the control group was treated with comprehensive drug treatment. The levels of TBIL, MDA, neurofunctional molecules, liver and kidney functional molecules were detected before and after treatment. Results: With one case in the intervention group being ineffective, the total effective rate was 97.78% (44/45), significantly higher than that in the control group (χ2=10.88, P=0.001). There was no difference in serum TBIL content between the two groups before treatment; After treatment, the serum TBIL content of the intervention group was significantly lower than that of the contro group (P<0.05). Before the treatment, there was no significant difference in the content of α - glutathione-s transferase and serum glutamate dehydrogenase between the two groups; after the treatment, the content of serum glutamate dehydrogenase in the intervention group was lower than that in the control group (P<0.05). There was no difference in serum MDA between the two groups before treatment, while, After treatment, the serum MDA content of the combined group decreased significantly, which was lower than that of the drug group (P<0.05). There was no difference in serum NSE content between the two groups before treatment; after treatment, the serum NSE content of the intervention group was significantly lower than that of the control group (P<0.05). Conclusion: In the treatment of hyperbilirubinemia, the combination of blue light irradiation and comprehensive treatment has a good effect. It not only effectively reduces the bilirubin content, but also protects children's nerve function, which has an extremely important clinical application value. 2020 Vol. 26 (8): 1273-1276 [Abstract] ( 114 ) HTML (1 KB)  PDF (1194 KB)  ( 258 ) 1277 Expression of MicroRNA-145 in Peripheral Blood of Children with Lower Respiratory Tract Infection of Respiratory Syncytial Virus and Its Association with Th1/Th2 Balance MAI Langjun, ZHAI Jiayu, LIN Lieju, et al DOI: 10.3969/j.issn.1006-6233.2020.08.011 Objective: To explore the relationship between miR-145 and Th1/Th2 balance by detecting the levels of miR-145 and Th1/Th2 cytokines in peripheral blood of children with lower respiratory tract infection caused by respiratory syncytial virus (RSV). Methods: 60 children with RSV bronchiolitis and pneumonia who were admitted to our hospital from June 2018 to November 2019 were selected as the experimental group, and 60 healthy children who had health examination in our hospital during the same period were selected as the control group. Real-time quantitative PCR (qRT-PCR) was adapted to detect the expression of miR-145 in peripheral blood mononuclear cells of all subjects, Th1 subgroup and Th2 percentage were detected by double fluorescence flow cytometry, and the expression levels of Interleukin-2(IL-2), Interleukin-4(IL-4), Interferon-γ(IFN-γ) and Interleukin-5(IL-5) in plasma of all subjects were detected by enzyme-linked immunosorbent assay (ELISA). Results: The expression level of miR-145 in the experimental group was significantly higher than that in the control group (P<0.05); the percentage of Th1 cells and Th1/Th2 ratio in the experimental group were significantly lower than those in the control group (P<0.05), and there was no significant difference between the two groups (P>0.05); the expression level of IL-2 and IFN-γ in plasma of experimental group was significantly lower than that of control group, and the expression level of IL-4 and IL-5 was significantly higher than that of control group (P<0.05); the levels of miR-145 of the experimental group were negatively correlated with the levels of IL-2, IFN-γ, Th1/Th2 ratio (P<0.05), and positively correlated with the levels of IL-4 and IL-5 (P<0.05). Conclusion: The up-regulation of miR-145 expression in peripheral blood of children with RSV lower respiratory tract infection is closely related to Th1/Th2balance. 2020 Vol. 26 (8): 1277-1280 [Abstract] ( 94 ) HTML (1 KB)  PDF (1199 KB)  ( 234 ) 1281 The Association Between the Expressions of Serum S-CD105 VEGF MMP9 and Recurrence of Non-small Cell Lung Cancer After Radical Operation LI Yanming, HAO Yanbing, YANG Jilei, et al DOI: 10.3969/j.issn.1006-6233.2020.08.012 Objective: To investigate the relationship between the expression of serum soluble CD105 (S-CD105), vascular endothelial growth factor (VEGF), matrix metalloproteinase 9 (MMP9) and recurrence of non-small cell lung cancer (NSCLC) after radical operation. Methods: 102 patients with NSCLC treated in our hospital from September 2014 to October 2017 were selected as the study subjects, who were followed up for 2 years. They were divided into recurrence group (n=44) and non-recurrence group (n=52) based on the recurrence. Another 60 healthy persons were selected as the control group during the same period. The levels of serum S-CD105, VEGF and MMP9 were detected by ELISA, the changes of levels of serum S-CD105, VEGF and MMP9 were observed, the recurrence rates of patients with different levels of S-CD105, VEGF, MMP9S-CD105, VEGF and MMP9 were observed, and the predictive value of the expression of three factors for recurrence after NSCLC and the influencing factors were analyzed. Results: The levels of serum S-CD105, VEGF and MMP9 in the relapsed group were significantly higher than those in the non-relapsed group and the control group (P<0.05). The recurrence rate of patients with high expressions of serum S-CD105, VEGF and MMP9 was higher than that of patients with low expressions (P<0.05). MMP9 had the highest specificity but lower sensitivity in predicting recurrence of NSCLC, and AUC predicted by VEGF was the highest, and the sensitivity and specificity were slightly higher than those predicted by S-CD105. TNM stage, chemotherapy cycle, S-CD105, VEGF and MMP9 were independent risk factors for recurrence of NSCLC. Conclusion: Serum S-CD105, VEGF and MMP9 are highly expressed in patients with recurrent NSCLC, postoperative recurrence rate of patients with high expressions of the three factors is high. These three factors have certain predictive values for postoperative recurrence and are independent risk factors for postoperative recurrence. 2020 Vol. 26 (8): 1281-1285 [Abstract] ( 112 ) HTML (1 KB)  PDF (1425 KB)  ( 282 ) 1286 Efficacy of Levamisole Combined with Glucocorticoid in Treating Primary Nephrotic Syndrome of Children and Its Effects on Serum SPD-1 and SPD-L1 Levels LI Xiaomei, DENG Xiulan DOI: 10.3969/j.issn.1006-6233.2020.08.013 Objective: To study the curative efficacy of levamisole combined with glucocorticoid in treatment of children with primary nephrotic syndrome and its effects on serum soluble programmed death receptor 1(sPD-1) and soluble programmed death receptor ligand 1(sPD-L1) levels. Methods 90 children with primary nephrotic syndrome who received therapy from January 2016 to October 2018 in our hospital were selected, according to the random number table, and they were divided into the observation group and the control group, 45 cases in each. Besides basic treatment, the control group was treated with prednisone acetate tablets, and the observation group was additionally administered levamisole. After continuous treatment of 8 weeks, the clinical efficacy, the changes of the 24-hour urine protein quantity, albumin(ALB), total cholesterol (TC), triglyceride (TG), immunoglobulin(Ig), T lymphocyte subsets, serum sPD-1, sPD-L1 and recurrence rate of 6 months were compared between the two groups. Results: After treatment, the total effective rate of the observation group was significantly higher than that the control group(95.56%vs82.22%)(P＜0.05); the 24-hour urine protein quantity, TC, TG in the observation group were (0.35±0.08)g, (5.05±0.74)mmol/L and (1.35±0.20)mmol/L, which were significantly lower than the control group (0.74±0.19)g, (5.82±0.69)mmol/L and (1.69±0.23)mmol/L. The ALB in observation group were (38.94±3.71)g/L, which were significantly higher than the control group (34.12±3.90)g/L, anf the difference was statistically significant(P<0.05); the IgG, IgA, IgM, CD4+ and CD4+/CD8+ were (9.21±1.68)g/L, (0.90±0.15)g/L, (1.48±0.12)g/L, (40.23±2.17)%, (1.52±0.19), which were significantly higher than the control group(7.65±1.84)g/L, (0.79±0.13)g/L, (1.33±0.09)g/L, (37.46±2.29)%, (1.38±0.15), and the difference was statistically significant(P<0.05); the serum sPD-1, sPD-L1 in the observation group were (1.11±0.25)ng/ml and (69.56±7.40)pg/ml, which were significantly lower than the control group(1.63±0.36)ng/ml and (82.36±8.27)pg/ml, the difference was statistically significant(P<0.05); the follow-up for 6 months showed that the recurrence rate in the observation group was significantly lower than that in the control group(7.00%vs20.00%)(P<0.05). Conclusion: Levamisole combined with glucocorticoid is effective for children with primary nephrotic syndrome, which can effectively improve the laboratory indexes and reduce the recurrence rate. It's internal mechanism may regulate the immune function and serum sPD-1 and sPD-L1, and it's worth popularizing. 2020 Vol. 26 (8): 1286-1290 [Abstract] ( 203 ) HTML (1 KB)  PDF (1217 KB)  ( 634 ) 1291 An Analysis of the Effects of MicroRNA-663b on Cell Activity and Apoptosis of Human Intervertebral Disc Degeneration Nucleus Pulposus Cells Induced by Low Glucose WANG Peng, ZUO bin, TANG Jiaguo, et al DOI: 10.3969/j.issn.1006-6233.2020.08.014 Objective: To study the regulatory mechanism of miR-663b on the proliferation and apoptosis of human intervertebral disc degeneration nucleus pulposus cells induced by low glucose. Methods: The expression of miR-663b in degenerative nucleus pulposus cells of human disc herniation and glucose (5, 1 mmol/L) -treated discs was detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction (qRT-PCR) experiments. The low glucose group + miR-NC group (transfected with miR-NC), the low glucose group + miR-663b group (transfected with miR-663b mimics), and the low glucose group + anti-miR-NC group (transfected with anti-miR -NC), low glucose group + anti-miR-663b group (transfected with anti-miR-663b), divided with the plastid method, were transfected into 1 mmol/L treated disc degeneration nucleus pulposus cells. Cell Counting Kit (CCK-8) method, flow cytometry, and western blot were used to detect the absorbance (OD) value of cells, the apoptosis rate of cells, and cell cycle-dependent protein kinase inhibitor 1A (P21) , protein expression of cysteine aspartic protease (Caspase-3) in cells. Results: The expression of miR-663b in tissues of human disc herniation and 1 mmol/L glucose (low glucose group) treated disc degeneration nucleus pulposus cells were significantly reduced (P<0.05). The cells of the low glucose group were inhibited from proliferating, their apoptotic capacity was improved, and the protein expression of P21 and Caspase-3 was increased. The expression of miR-663b was significantly increased in the low-glucose group cells overexpressing miR-663b, the cell proliferation ability was also significantly increased, and the apoptotic capacity was significantly reduced. The protein expression levels of P21 and Caspase-3 were inhibited, while miR-663b's low-glucose group cells had opposite effect. Conclusion: MiR-663b can promote the proliferation of human intervertebral disc degeneration nucleus pulposus cells induced by low glucose and inhibit apoptosis. 2020 Vol. 26 (8): 1291-1295 [Abstract] ( 97 ) HTML (1 KB)  PDF (1736 KB)  ( 230 ) 1296 Effects of Magnesium Sulfate, Nifedipine Tablets Combined with Diazepam on Blood Pressure SEng and SFlt-1 and Pregnancy Outcomes in Patients with Severe Preeclampsia WANG Hong, YANG Wen, ZHOU Yan, et al DOI: 10.3969/j.issn.1006-6233.2020.08.015 Objective: To explore the effects of magnesium sulfate, nifedipine tablets (Nf) combined with diazepam on blood pressure, serum soluble endoglin (sEng) and soluble vascular endothelial growth factor receptor 1 (sFlt-1) and pregnancy outcomes in patients with severe preeclampsia (S-PE). Methods: A total of 160 S-PE patients admitted to our hospital from June 2018 to June 2019 were selected for study. They were divided into control group and study group in a 1: 1 ratio according to the simple random number table method, with 80 cases in each group. Patients in control group were treated with magnesium sulfate + Nf while patients in study group were additionally given diazepam on the basis of control group. The clinical efficacy after 1 week of treatment and 24h blood pressure [systolic blood pressure (SBP), diastolic blood pressure (DBP)] and serum indicators (sEng, sFlt-1) before treatment and 1 week after treatment were compared between the two groups, and the pregnancy outcomes and occurrence of adverse drug reactions during treatment were recorded. Results: 1 week after treatment, the total effective rate in study group was significantly higher than that in control group(92.50% vs 81.25%) (P<0.05). The levels of 24h SBP and 24h DBP, serum sEng and sFlt-1 in the two groups were significantly decreased compared with those before treatment, and the above indicators in study group were significantly different from those in control group at the same period (P<0.05). The pregnancy outcome follow-up revealed that in study group it was better than in control group (P<0.05). The adverse drug reaction rate in study group was significantly lower than that in control group during treatment (P<0.05). Conclusion: For patients with severe preeclampsia, the application of magnesium sulfate, nifedipine tablets combined with diazepam can effectively improve the blood pressure, alleviate vascular endothelial damage and promote pregnancy outcomes, and it has higher safety and better application value. 2020 Vol. 26 (8): 1296-1299 [Abstract] ( 144 ) HTML (1 KB)  PDF (1192 KB)  ( 300 ) 1300 Detection of Plasma PCT, NLR and MCP-1 in the Diagnosis of Liver Cirrhosis Complicated with Spontaneous Bacterial Peritonitis and the Prognosis Evaluation XU juan, LIU xiaojing, QIN Lijun DOI: 10.3969/j.issn.1006-6233.2020.08.016 Objective: To investigate the detection of plasma procalcitonin (PCT), neutrophil-to-lymphocyte ratio (NLR) and monocyte chemotactic protein-1 (MCP-1) in the diagnosis of liver cirrhosis with spontaneous bacterial peritonitis (SBP) and the value of prognosis evaluation. Methods: A total of 146 patients with liver cirrhosis and ascites treated in our hospital from December 2017 to December 2018 were selected and divided into SBP group (n=76) and non-SBP group (n=70) according to clinical manifestations and laboratory examination results. Another 51 healthy examinees in the same period in our hospital were randomly selected as control group. The levels of plasma PCT, NLR and MCP-1 were measured among the three groups, and receiver operating characteristic (ROC) curve was plotted to analyze the diagnostic value of PCT, NLR and MCP-1 on liver cirrhosis with SBP. The changes of plasma PCT, NLR and MCP-1 before and after treatment were analyzed in SBP group, and the value of PCT, NLR and MCP-1 in the prognosis evaluation of liver cirrhosis with SBP was investigated. Results: The levels of plasma PCT, NLR and MCP-1 in SBP group were significantly higher than those in non-SBP group and control group (P<0.05), and the level of plasma MCP-1 in non-SBP group was significantly higher than that in control group (P<0.05), and there were no significant differences in the PCT and NLR compared with those in control group (P>0.05). ROC curve results showed that when the optimal cutoff value of PCT was 0.94ng/ml, the AUC for diagnosis of liver cirrhosis with SBP was 0.850 (95% CI: 0.787-0.912), and when the optimal cutoff value of NLR was 13.72, the AUC was 0.721 (95% CI: 0.639-0.803), and when the optimal cutoff value of MCP-1 was 0.69μg/L, the AUC was 0.810 (95% CI: 0.741-0.879), and the AUC of combination of the three was 0.866 (95% CI: 0.807-0.926). The AUC, sensitivity and specificity of the combined diagnosis were better than those of PCT, NLR or MCP-1 alone. The levels of plasma PCT, NLR and MCP-1 in patients with liver cirrhosis and SBP after treatment were significantly lower than those before treatment (P<0.05). Conclusion: The levels of plasma PCT, NLR and MCP-1 are abnormally elevated in patients with liver cirrhosis and SBP. Detection of plasma PCT, NLR and MCP-1 has certain clinical value in the early diagnosis and prognosis evaluation of liver cirrhosis and SBP. 2020 Vol. 26 (8): 1300-1303 [Abstract] ( 82 ) HTML (1 KB)  PDF (1330 KB)  ( 287 ) 1304 Effects of Propranolol Combined with Methimazole on Serum PTH OCN ALP Ca and P Levels of Patients with Hyperthyroidism XU Wen, CHEN Chaocong, XU Wenjuan DOI: 10.3969/j.issn.1006-6233.2020.08.017 Objective: To analyze the effects of propranolol combined with methimazole on serum parathyroid hormone (PTH), osteocalcin (OCN), alkaline phosphatase (ALP), calcium (Ca) and phosphorus (P) levels in patients with hyperthyroidism. Methods: A total of 200 patients with hyperthyroidism who were admitted to the hospital from December 2017 to December 2018 were selected as subjects. They were divided into the observation group (n=100) and the control group (n=100) with simple random method. The control group was treated with methimazole while the observation group was treated with propranolol combined with methimazole. The clinical curative effect, serum PTH, OCN, ALP, Ca and P levels, the incidence of adverse reactions and recurrence rate were compared between the two groups. Results: After treatment, the levels of serum PTH and Ca in the observation group were significantly higher than those in the control group. The levels of OCN, ALP and P were significantly lower than those in the control group (P<0.05). The total effective rate of the observation group after treatment was significantly higher than that of the control group (88.00% vs 70.00%) (P<0.05). The incidence of adverse reactions in the observation group after treatment was significantly lower than that in the control group (6.67% vs 24.44%) (P<0.05).The recurrence rate of the observation group was 3.00%, which was significantly lower than that of the control group (11.00%) (P<0.05). Conclusion: The clinical curative effect of propranolol combined with methimazole is obvious in the process of hyperthyroidism treatment. The comprehensive treatment can reduce the levels of serum OCN, ALP and P, and increase the levels of PTH and Ca. 2020 Vol. 26 (8): 1304-1306 [Abstract] ( 85 ) HTML (1 KB)  PDF (1170 KB)  ( 308 ) 1307 Efficacy of Valproic Acid Combined with Ziprasidone on Bipolar Disorder and Its Effect on Serum Total Bilirubin Albumin and Inflammatory Factors LI Yan, HE Xiance DOI: 10.3969/j.issn.1006-6233.2020.08.018 Objective: To study clinical evaluation of magnesium valproate sustained release tablets combined with ziprasidone in treatment of bipolar disorder and effect on serum total bilirubin, albumin and inflammatory factors. Methods: 90 cases with bipolar disorder from October 2014 to October 2016 in our hospital were researched, and according to the random number table they were divided into treatment group and control group, 45 cases in each. Magnesium valproate sustained release tablet was administered to the control group, while the observation group was treated with magnesium valproate sustained release tablets combined with ziprasidone. They were compared on clinical treatment effect, serum albumin, total bilirubin and inflammatory factor levels, score of BRMS, Quality of Life and HAMD and safety. Results: After treatment, the difference between the total effective rate in treatment group (93.33%) and the total effective rate in the control group (77.78%) was statistically significant (P<0.05). The levels of serum albumin and total bilirubin in the treatment group were significantly higher than those in the control group (P<0.05), while the levels of inflammatory factors were significantly lower in the treatment group than in the control group (P<0.05). Scores of BRMS and HAMD in both groups were significantly decreased, while the life quality score was significantly increased, and the improvement of mania scale, Hamilton depression scale and life quality score in the treatment group was significantly better than that in the control group(P<0.05). Conclusion: The efficacy of magnesium valproate sustained release tablets combined with ziprasidone in the treatment of bipolar disorder was significant in reducing the score of BRMS and HAMD and improving serum total bilirubin, albumin, inflammatory factor levels, and it has less adverse reactions, being safe and reliable. 2020 Vol. 26 (8): 1307-1311 [Abstract] ( 104 ) HTML (1 KB)  PDF (1207 KB)  ( 634 ) 1312 Analysis of Effect of Modified Nuss in the treatment of Children's Funnel Chest Effect and Postoperative Complications LI Jibin, SUN Yufang DOI: 10.3969/j.issn.1006-6233.2020.08.019 Objective: To analyze the effect of modified minimally invasive funnel chest correction (Nuss) in the treatment of children's funnel chest and the factors that lead to postoperative complications. Methods: From February 2016 to February 2018, 90 children undergoing Nuss operation in our hospital were analyzed retrospectively. the results were observed and divided into groups according to whether the complications occurred after operation. The general data of patients were analyzed by univariate analysis. The statistically significant indexes were included in multivariate Logistic regression, and the independent risk factors leading to complications were analyzed. Results: The operation was successfully completed in 90 cases. A total of 21 cases had postoperative complications, but after corresponding treatment, there was no serious problem when they were discharged from hospital. All the children were followed up. During the follow-up period, there was no obvious discomfort and no plate displacement occurred. Univariate analysis showed that age, the symmetry of funnel chest, the number of plates inserted and the time of operation were the influencing factors of complications, and the difference was statistically significant (P<0.05). Multivariate Logistic regression showed that the operation time was too long, asymmetric funnel chest and the number of plates placed was an independent risk factor for complications, and the difference was statistically significant. Learning significance (P<0.05). Conclusion: Nuss operation has a good effect on the treatment of pectus excavatum in children. The long operation time, asymmetric pectus excavatum and the number of plates placed are independent risk factors for postoperative complications. In clinical practice, high attention should be paid to this type of children to prevent serious complications caused by the above factors. 2020 Vol. 26 (8): 1312-1316 [Abstract] ( 89 ) HTML (1 KB)  PDF (1194 KB)  ( 254 ) 1317 The Curative Effect of Early Interventional Embolization on the Treatment of Intracranial Aneurysm and Its Effect on the Patients' Neurological Function Mental State and Activities of Daily Living LI Jiangtao, YANG Hua, FAN Yujun DOI: 10.3969/j.issn.1006-6233.2020.08.020 Objective: To explore the therapeutic effect of early interventional embolization in the treatment of intracranial aneurysms, and to observe the effects of early interventional embolization on the neurological function, mental state and activities of daily living of the patients. Methods: The patients with intracranial aneurysm (n=84) from March 2016 to March 2019 were selected as experimental subjects, and divided into two groups with the random digital table method. The control group (n=42) was treated with advanced interventional embolization, and the observation group (n=42) was treated with early intervention. The clinical effect, complication rate, prognosis, neurological function score (NIHSS), daily life ability score (ADL) and mental state score (KPS) were observed before and after treatment. Results: The total effective rate of the observation group was 95.24% in comparison with 71.43% of the total effective rate of the control group(χ2=8.571, P<0.05).Compared with 14.29% of the mortality rate and 23.81% of the disability rate in the control group, the mortality rate in the observation group was 2.38% and the disability rate was 4.76% The difference between the two groups was obvious and statistically significant(P<0.05). The results of repeated ANOVA showed that the NHISS score, KPS score, ADL score, intra-subject effect and inter-subject effect of different time points were statistically significant(P<0.05).The NHISS score of the patients in the observation group was (14.50±1.81), which was significantly lower than that in the control group [(20.19±4.24), P<0.05].The scores of KPS (86.05±6.84) were higher than that in the control group[(78.36±7.58), P<0.05]. The score of ADL (78.45±7.62) was significantly higher than that in the control group[(61.57±8.06), P<0.05], and the MMSE score (26.52±4.17) was significantly higher than that in the control group[(19.86±4.71), P<0.05].Compared with 26.19% of the total complications in the control group, the total incidence of complications in the observation group was 7.14%. There was significant difference between the two groups(χ2=5.486, P<0.05). Conclusion: Compared with advanced interventional embolism, early interventional embolism has a better effect on intracranial aneurysm, which can significantly improve the neurological status of patients, improve the ability of daily living, restore neurological function, effectively reduce complications, and the prognosis of patients is good. 2020 Vol. 26 (8): 1317-1320 [Abstract] ( 107 ) HTML (1 KB)  PDF (1195 KB)  ( 502 ) 1321 Expression and Significance of Mismatch Repair Protein in Malignant Epithelial Tumors in Different Parts of the Large Intestine DUAN Yongdong, DAI Junli DOI: 10.3969/j.issn.1006-6233.2020.08.021 Objective: To investigate the expression of mismatch repair protein (MMR) in colorectal cancer tissues in different parts of the large intestine and the significance of MMR expression in colorectal cancer. Methods: The expression and significance of mismatch repair proteins (msh1, MSH2, MSH6, and PMS2) in colorectal cancer tissues were analyzed by immunohistochemical method (SP method), and the expression significance of MMR protein in different parts of colorectal cancer was analyzed. Deletion of one or more of msh1, MSH2, MSH6 and PMS2 proteins was determined as mismatch repair gene defect (dmmr), and all expression levels were determined as mismatch repair gene integrity (PMMR). Results: In 168 cases of colorectal cancer, there were 141 cases (83.93%) of PMMR, 27 cases (16.07%) of dmmr, 56 cases (71.79%) of MMR protein expression loss group (dmmr) and 22 cases (28.21%) of MMR protein non deletion group (PMMR) in 78 cases of normal colorectal mucosa adjacent to carcinoma (P<0.05). In 47 cases of right colon cancer, there were 19 cases (40.42%) of dmmr, 5 cases (5.43%) of left colon cancer, 3 cases (10.34%) of 29 cases of rectal cancer. Furthermore, there was a certain relationship between dmmr and clinicopathological features of colorectal cancer. The dmmr in the group with lymph node metastasis and invasion into the muscular layer was significantly lower than that in the group without lymph node metastasis and the group with invasion of muscle layer (P<0.05). Conclusion: The expression of mismatch repair protein in colorectal cancer is higher than that in normal intestinal mucosa, and the expression of mismatch repair protein is different in different parts of colorectal cancer. Different status of mismatch repair protein is related to the progress of colorectal cancer, and suggests that the prognosis of colorectal cancer with mismatch repair protein deficiency may be better. 2020 Vol. 26 (8): 1321-1323 [Abstract] ( 154 ) HTML (1 KB)  PDF (2535 KB)  ( 347 ) 1324 Long-term Visual Recovery, Changes of Corneal Endothelial Cells and Related Factors in Patients with Cataract Surgery Assisted by Femtosecond Laser DUAN Qiong, et al DOI: 10.3969/j.issn.1006-6233.2020.08.022 Objective: To investigate the long-term vision recovery, corneal cell changes and related influencing factors after femtosecond laser assisted cataract surgery. Methods: 73 cases of cataract patients who underwent femtosecond laser assisted cataract surgery in our hospital from August 2015 to July 2017 were selected as the research objects. The changes of visual acuity and corneal endothelial cell count were observed before operation, 1 day after operation, 1 week after operation, 1 month after operation and 6 months after operation; According to the loss of corneal endothelial cells, they were divided into severe loss group and general loss group. Firstly, the variables with statistical significance were included in logistic regression and the univariate analysis was used to analyze the risk factors of severe corneal endothelial cell loss. Results: There were significant differences in myopia, hyperopia and corneal endothelial cell count at different time points (P<0.05). The visual acuity of naked myopia and naked hyperopia at 1 week, 1 month and 6 months after operation was significantly higher than that before operation. 1 week after operation was significantly higher than 1 day after operation. 1 month after operation was significantly higher than 1 day and 1 week after operation. 6 months after operation was significantly higher than 1 day, 1 week, 1 month and 6 months after operation, the difference was statistically significant (P<0.05). Conclusion: Femtosecond laser-assisted cataract surgery can improve the visual acuity of patients, but there is corneal loss in patients. Age, preoperative lens nucleus hardness grade, preoperative anterior chamber depth and preoperative axial length are the influencing factors and independent risk factors of severe corneal loss, which should be paid special attention to in clinical. 2020 Vol. 26 (8): 1324-1328 [Abstract] ( 91 ) HTML (1 KB)  PDF (1203 KB)  ( 332 ) 1329 An Analysis of Diagnostic Value on Etiological Classification of PCT and CRP/Alb Ratio in Serum of Patients Suffering from SAP XU Mojun, SUN Xiangjun DOI: 10.3969/j.issn.1006-6233.2020.08.023 Objective: To analyze diagnostic value on etiological classification for stroke associated pneumonia (SAP), by detection on the PCT and CRP/Alb ratio in serum of patients suffering from stroke. Methods 368 patients suffering from SAP hospitalized from January 2017 to November 2019 were selected, and then, based on the results of sputum culture, were divided into the positive group(n=211) and the negative group(n=157). The patients who were hospitalized with stroke but without infection were made the control group(n=157). The clinical data of each group were analyzed retrospectively, and the PCT and CRP/Alb ratio in serum , the results of sputum culture and the serological examination of the pathogen as well. Results:Compared with the control group, the PCT and CRP/Alb ratio in serum of the patients in the observation group were significantly elevated(0.73±0.22vs0.07±0.03; 0.87±0.23vs0.48±0.19 P<0.05). Among the 368 patients suffering from SAP, 211(57.3%)sputum cultures were positive. Gram-positive bacteria were 79(37.3%), Gram-negative bacteria, 91(43.0%), fungus, 17(8.2%) , the rest of sputum cultures, 24(11.4%), mainly including Escherichia coli, Klebsiella Pneumoniae, acinetobacter Bowman, Staphylococcus Aureus, Streptococcus Pneumoniae, Candida Albicans, Candida Tropicalis, Candida Glabrata, et al. The PCT and CRP/Alb ratio in serum of the patients the positive group of sputum bacteria culture were higher than the negative group(1.19±0.34vs0.62±0.27; 2.19±0.45vs1.49±0.28 P<0.05). After examining the serological identification results of the patients in the observation group, 243 cases (66.3%) had bacterial infection, 56 cases (15.2%) had viral infection, 43 cases (11.7%) had mycoplasma infection, 26 (7.1%) of the rest were found with other infections. The PCT and CRP/Alb ratio in serum of the patients of the bacterial infection group were significantly higher than the viral infection group and the mycoplasma infection group(1.26±0.28vs0.51±0.14vs 0.12±0.03;2.37±0.69vs1.79±0.56vs0.38±0.24 P<0.05). Conclusion: The most common pathogens in patients with SAP were bacteria, virus, mycoplasma and fungi. Serum CRP/Alb ratio has a certain diagnostic value on etiological classification in predicting the occurrence of SAP, It is helpful to the etiological diagnosis and it guides the clinical treatment. 2020 Vol. 26 (8): 1329-1332 [Abstract] ( 93 ) HTML (1 KB)  PDF (1198 KB)  ( 261 ) 1333 A Study of Gd-EOB-DTPA Dynamic Contrast Enhanced MRI in Quantitatively Assessing Liver Fibrosis in Rabbits ZHANG Liping, WU Xiuli, HUANG Baosheng, et al DOI: 10.3969/j.issn.1006-6233.2020.08.024 Objective: To explore quantitative parameters obtained by dynamic contrast-enhanced magnetic resonance imaging (DCE MRI) with gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA) in diagnosing and discriminating liver fibrosis (LF) in a rabbit model. Methods: LF group was established in 60 rabbits by the injection of 50% CCl4 oil solution, whereas 20 rabbits injected with normal saline served as the control group. 15 objects in the LF group and 5 in the control group were randomly selected at the end of the 4th, 5th, 6th, 15th week after injection of 50% CCl4 oil solution, respectively. All rabbits underwent pathological examination to determine the LF stage using the Metavir classification system. DCE MRI was performed, and quantitative parameters, including trans, Kep , Ve, Vp and Re were measured and compared among the different LF stages. Results: In all, 16, 14, 12, 10, 11 rabbits of stage F0, F1, F2, F3 and F4, respectively, were included in this study. trans (r=0.913) increased, Kep (r =-0.757) and Re (r =-0.804) decreased with LF stage progression (P<0.001), while no significant correlation was found for Ve or Vp. The AUC of trans was higher than that of other quantitative parameters, with an AUC of 0.96, 0.96, 0.96 and 0.99 for staging ≥F1、≥F2、≥F3、≥F4, respectively. Conclusion: Gd-EOB-DTPA DCE MRI parameters are closely associated with LF progression, indicating that this may be a promising method for quantitatively differentiating early-stage LF. 2020 Vol. 26 (8): 1333-1337 [Abstract] ( 86 ) HTML (1 KB)  PDF (1628 KB)  ( 267 ) 1338 Observation on Therapeutic Effects of Endoscopic Submucosal Dissection in Patients with Early Gastrointestinal Cancer and Precancerous Lesions JIN Yingchun, ZHAO Jin, ZHOU Jin DOI: 10.3969/j.issn.1006-6233.2020.08.025 Objective: To investigate the therapeutic effects of endoscopic submucosal dissection (ESD) in patients with early gastrointestinal cancer and precancerous lesions. Methods: 77 patients with early gastrointestinal cancer and precancerous lesions who underwent endoscopic mucosal resection (EMR) or ESD in the Department of Gastroenterology of the hospital from August 2015 to August 2017 were selected as subjects. The size and type of lesions in the two groups were recorded. The complete resection rate, en bloc resection rate, operation time, length of hospital stay, intraoperative and postoperative complications, and follow-up recurrence were recorded. Results: There were 32 patients (35 lesions) in ESD group and 45 patients (50 lesions) in EMR group. The lesion diameter in ESD group was significantly larger than that in EMR group (P<0.05). The complete resection rate of ESD group and EMR group was 97.14% and 68.89%, respectively, and the whole resection rate was 100.00% and 75.56%, respectively, with significant difference between the two groups (P<0.05). There was no significant difference in the complete resection rate and the whole resection rate of lesions ≤ 20 mm between the two groups (P>0.05). The complete resection rate and the total resection rate of lesions ≥ 20 mm in ESD group were significantly higher than those in EMR group (P<0.05). The operation time in ESD group was higher than that in EMR group (P<0.05). There was no significant difference in bleeding, perforation and postoperative recurrence between the two groups (P>0.05). Conclusion: The curative effect and safety of ESD are good in the treatment of early gastrointestinal cancer and precancerous lesions. Compared with EMR, the resection effect of ESD is better. However, the duration of ESD is longer and there's a high risk for the surgeon. Clinically, the patient's clinical data, condition of lesions and indications can be combined for choosing surgical method reasonably. 2020 Vol. 26 (8): 1338-1342 [Abstract] ( 123 ) HTML (1 KB)  PDF (1216 KB)  ( 277 ) 1343 Contrast-Enhanced Ultrasound Combined with Quantitative Analysis in the Diagnosis of Benign and Malignant Thyroid Nodules TA Na, WANG Xia, JING Jiangxin DOI: 10.3969/j.issn.1006-6233.2020.08.026 Objective: To analyze the application of contrast-enhanced ultrasound combined with quantitative analysis in the diagnosis of benign and malignant thyroid nodules. Methods:Retrospective analysis was conducted of clinical data of 72 patients with thyroid nodules who underwent contrast-enhanced ultrasound examination in our hospital from May 2019 to December 2019. The characteristics of contrast-enhanced ultrasound enhancement mode of benign and malignant thyroid nodules were observed. The time-intensity curve was used to analyze blood flow perfusion parameters such as contrast medium transit time, peak intensity, peak time, and area under the curve. Using the postoperative pathological results as a control, the diagnostic value of contrast-enhanced ultrasound combined with quantitative analysis technology was analyzed. Results: Contrast-enhanced ultrasound showed that among 72 patients with thyroid nodules, 43 were benign nodules and 29 were malignant nodules. The pathological types of benign nodules are thyroid adenomas and nodular goiters. Their contrast-enhanced ultrasound features are uniform enhancement or no enhancement inside, peripheral ring enhancement, and clear boundaries. The pathological types of malignant nodules are papillary thyroid carcinoma. Contrast-enhanced ultrasound is characterized by low or uneven enhancement, irregular morphology, and blurred borders. Postoperative pathology confirmed 47 benign nodules and 25 malignant nodules. The sensitivity of contrast-enhanced ultrasound in diagnosing malignant thyroid nodules was 88.00%, the specificity was 85.11%, and the accuracy was 86.21%.Compared with benign thyroid nodules, Peak and AUC in diagnosing malignant thyroid nodules were relatively lower, and had a later TIP (P<0.05). Compared with normal tissues surrounding thyroid malignant nodules, Peak and AUC of thyroid malignant nodules were lower and TIP was later (P<0.05). Compared with normal tissue around benign thyroid nodules, Peak and AUC of benign thyroid nodules were slightly lower, TIP was slightly later, and MTT was slightly slower, but there were no significant differences(P>0.05). Conclusion: Contrast-enhanced ultrasound combined with quantitative analysis technology can be used for the differential diagnosis of benign and malignant thyroid nodules, and the quantitative parameters Peak, AUC and TIP can reflect the vascular perfusion regularity of malignant thyroid nodules. 2020 Vol. 26 (8): 1343-1346 [Abstract] ( 97 ) HTML (1 KB)  PDF (1194 KB)  ( 303 ) 1347 An Analysis of the Effect of Extensive Hysterectomy Combined with Pelvic Lymphadenectomy on Cervical Cancer and the Influencing Factors of Postoperative Pelvic Floor Dysfunction LI Yuanyuan, YANG Daping, SUN Yanyan DOI: 10.3969/j.issn.1006-6233.2020.08.027 Objective: To investigate the effect of extensive hysterectomy combined with pelvic lymphadenectomy for cervical cancer, and to analyze the influencing factors of postoperative pelvic floor dysfunction (PFD). Methods: 63 patients with cervical cancer who were treated in our hospital from January 2015 to June 2019 were divided into study group (32 cases) and control group (31 cases) with random table method. All patients underwent extensive hysterectomy combined with pelvic lymphadenectomy. As to reatment, the study group took laparoscopy, and the control group took conventional open surgery. The intraoperative and postoperative conditions and complications were compared between the two groups. Binary logistic regression was used to analyze the risk factors of postoperative PFD. Results: The operation time, intraoperative blood loss, time to get out of bed, anal exhaust time, postoperative defecation time, and hospitalization time of the study group were significantly lower than those of the control group. The number of lymph node dissection in the study group was significantly higher than that of the control group. It is tatistical significance (P<0.05). The complication rate of the study was 56.25%, which was significantly lower than that of the control group (87.10%). The difference was statistically significant (P<0.05). A total of 38 cases of PFD occurred after operation. The factors of age, tumor diameter, presence or absence of ejaculation, and indwelling catheter were the influencing factors of postoperative PFD in patients with cervical cancer (P<0.05). Multivariate binary logistic analysis reveals that age ≥ 45 years, tumor diameter ≥ 4cm, menopausal, urethral catheter indwelling time ≥ 7d were risk factors for postoperative PFD in patients with cervical cancer (P<0.05). Conclusion: Laparoscopic extensive hysterectomy combined with pelvic lymphadenectomy for cervical cancer patients has a good effect, but incidence rate of postoperative PFD is high. Proper measures should be taken to aged menopausal patients whose tumor diameter is too large, and urethral catheter is long indwelled. 2020 Vol. 26 (8): 1347-1351 [Abstract] ( 125 ) HTML (1 KB)  PDF (1201 KB)  ( 373 ) 1352 Distribution and Drug Resistance of Nosocomial Pneumonia Pathogens After Craniocerebral Surgery MU Huaibo, SONG Chao DOI: 10.3969/j.issn.1006-6233.2020.08.028 Objective: To understand pathogens distribution and drug resistance of HAP after craniocerebral operation, and to provide evidence for clinical anti-infection empirical treatment. Methods: A retrospective analysis was conducted on the bacteria and their drug-resistance of urinarytract infections isolated from the sputum specimens of 60 pneumonia patients from January 2018to August 2018 after their intracranial surgery. Results: 185 strains of pathogens were found, including 165 strains of gram negative bacteria, accounting for 89.19%, gram positive bacteria, 18 strains, accounting for 9.73% and 2 strains of fungi, accounting for 1.08%.The detection rate of the top five pathogens were 57 strains of Pseudomonas aeruginosa, accounting for 30.81%, 32 strains of Klebsiella pneumonia, accounting for 17.3%, Bauman 27 Acinetobacter strains, accounting for 14.59%, 25 strains of Escherichia coli, accounting for 13.51%, and 12 strains of Serratia liquefaciens, accounting for 6.49%. Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Serratia liquefaction have better sensitivity to imipenem, meropenem, cefoperazone sulbactam, piperacillin tazobactam, amikacin, ciprofloxacin and cefepime. Acinetobacter baumannii only has good sensitivity to cefoperazone sulbactam, and has resistance to other drugs. Vancomycin-resistant strains of Staphylococcus aureus was not found, and it has a good sensitivity to doxycycline and minocycline. Conclusion: After craniocerebral operation, most common infection microorganism of HAP was Gram-negative bacteria, and parts showed multidrug resistance. 2020 Vol. 26 (8): 1352-1356 [Abstract] ( 80 ) HTML (1 KB)  PDF (1180 KB)  ( 276 ) 1357 An Evaluation of Intestinal Trefoil Factor on Early Intestinal Dysfunction in Septic Shock Patients and Its Association with Severity of the Disease WU Changhui, et al DOI: 10.3969/j.issn.1006-6233.2020.08.029 Objective: To investigate the value of the evaluation of intestinal trefoil factor (TFF3) in early intestinal dysfunction in septic shock patients and its relationship with the severity of the disease. Methods: From November 2016 to October 2019, 150 patients with septic shock within 24 hours after admission in the Department of critical medicine of Dazhou Central Hospital were selected as the study objects. According to whether early intestinal dysfunction was complicated during hospitalization, they were divided into non intestinal dysfunction group (n = 72) and intestinal dysfunction group (n = 78). Clinical data were collected and serum TFF3 was detected by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). Pearson test was used to analyze the correlations between serum TFF3 level and Acute Physiology And Chronic Health Evaluation Ⅱ (APACHE Ⅱ), C-reactive protein (CRP), blood urea nitrogen (BUN), blood creatinine (SCr) and gastrointestinal function score; multivariate logistic regression analysis of binary variables was used to screen the independent risk factors of early intestinal dysfunction in septic shock patients; and the ROC curve was drawn to evaluate the predictive values of APACHE Ⅱ score, SCr and TFF3 in patients with septic shock complicated with early intestinal dysfunction. Results: The APACHE Ⅱ score, levels of CRP, BUN, SCr and serum TFF3 of patients in intestinal dysfunction group were significantly higher than those of patients in non intestinal dysfunction group (P<0.05). There was a positive correlation between serum TFF3 level and APACHE Ⅱ score, CRP, BUN, SCr and gastrointestinal function score (P<0.05). Multivariate logistic regression analysis showed that the increases of APACHE Ⅱ score, SCr and serum TFF3 level were independent risk factors for septic shock complicated with early intestinal dysfunction (P<0.05). The ROC curve showed that the area under the curve (AUC) of serum TFF3 level in patients with septic shock complicated with early intestinal dysfunction was higher than that of APACHE Ⅱ score and SCr, and the sensitivity and specificity were 82.10% and 98.60% respectively when the best cutoff value was 12.97 ng/ml. Conclusions: The serum TFF3 level of septic shock patients with early intestinal dysfunction is increased, which can be used as a serum marker to evaluate the early intestinal dysfunction of septic shock patients. 2020 Vol. 26 (8): 1357-1361 [Abstract] ( 87 ) HTML (1 KB)  PDF (1408 KB)  ( 261 ) 1362 An Analysis of Diagnosing Pulmonary Ground Glass Nodules of Different Pathological Types Through High Quantitative Parameter of Resolution CT WEI Fang, KONG Xiangchen, TIAN Xiaolei DOI: 10.3969/j.issn.1006-6233.2020.08.030 Objective: To explore the value of high resolution CT (HRCT) in the differential diagnosis of different pathological types of lung ground glass nodules (GGN). Methods: 210 patients who were admitted to our hospital from Dec. 2017 to Dec. 2019 with lung GGN were selected as the research object, and all patients underwent imageological examination and pathological examination. Comparison was conduct among patients with different pathological types of lung GGN on differences of lesion size, 3 d and 2 d ratios, solid component size, shape, edge, burr, boundry points and lobulation, cavitation. Results: Pathological examination showed that among the 210 patients, pulmonary inflammation, AAH, AIS, MIA and IAC were 24, 30, 50, 62 and 44, respectively. Imaging data showed that the lung GGN presented burr, lobulation, cavitation sign, pleural depression sign, irregular lesion and fuzzy boundary, and the inflammatory group <AAH group <AIS group <MIA group <IAC group, with statistically significant differences (P<0.05).In addition, as to the size of lesions and the size of solid components, data showed the inflammatory group <AAH group <AIS group <MIA group <IAC group; the difference was statistically significant (P<0.05).There was no significant difference between the five groups (P>0.05).In addition, the ROC curve showed that the AUC of solid component size in diagnosing MIA and IAC lung GGN was 1.000 and 0.937, respectively, with high accuracy, sensitivity and specificity: MIA was 100% and 100%, IAC was 88.6% and 90.3%, with good diagnostic value. Conclusion: High resolution CT has important value in distinguishing pulmonary ground-glass nodules of different pathological types, and can be comprehensively evaluated according to qualitative and quantitative data of HRCT imaging characteristics. 2020 Vol. 26 (8): 1362-1366 [Abstract] ( 166 ) HTML (1 KB)  PDF (1949 KB)  ( 508 ) 1367 An Analysis of the Impact of Abnormal Sperm Morphology on the Outcome of Assisted Pregnancy and Neonatal Deformity Rate During the in Vitro Fertilization Cycle LIU Limin, et al DOI: 10.3969/j.issn.1006-6233.2020.08.031 Objective: To evaluate the effect of abnormal sperm morphology on the outcome of assisted pregnancy and the rate of neonatal deformities during the in vitro fertilization cycle. Methods: From January 2013 to January 2020, 1820 patients were selected who were accepting assisted pregnancy during the in vitro fertilization cycle for the first time in our reproductive medical center. The percentage of normal morphological sperm of patients was measured and they were divided into three groups (<1.0%, 1.0~4.0% and> 4.0%). The outcomes of assisted pregnancy among the three groups, including clinical pregnancy rate, live birth rate, and natural abortion rate, were compared. Then the safety, including preterm birth, infant weight and deformity rate, were further compared. Results: The three groups included 145, 726, and 949 cases, respectively. There was no significant difference in general information (age of women, body mass index, basic FSH, age of men and infertility years.) among the three groups before assisted pregnancy (P>0.05); the percentage of morphological sperm had no significant effect on the outcomes of assisted pregnancy, such as clinical pregnancy rate, live birth rate and spontaneous abortion rate. Follow-up of 777 deliveried couples showed that there was no statistical difference in preterm birth rate and infant weight among the three groups (P>0.05), and abnormal sperm morphology will not increase the deformity rate of the newborn. Conclusion: Abnormal sperm morphology did not affect the assisted pregnancy outcome (including clinical pregnancy rate, live birth rate and spontaneous abortion rate), nor the safety of the newborn (including premature birth, infant weight and deformity rate). The study could provide certain reference for the safety of patients with abnormal sperm morphology on selecting assisted pregnancy. 2020 Vol. 26 (8): 1367-1370 [Abstract] ( 88 ) HTML (1 KB)  PDF (1193 KB)  ( 288 ) 1371 Curative Effects of Lanthanum Carbonate in Maintenance Hemodialysis and Its Influence on Coronary Artery Calcification LI Wan, ZHOU Ping, LI Qin, et al DOI: 10.3969/j.issn.1006-6233.2020.08.032 Objective: To explore the effects of lanthanum carbonate (LC) in maintenance hemodialysis (MHD) and the influence on coronary artery calcification (CAC). Methods: A total of 105 hyperphosphatemia patients who underwent MHD and were admitted to the hospital from August 2018 to July 2019 were enrolled as study objects. They were divided into control group (n=52) and observation group ( n=53) by simple random grouping method. The former was treated with calcium carbonate, while the latter was treated with LC. The levels of serum calcium (Ca), serum phosphorus (P), intact parathyroid hormone (iPTH), bone alkaline phosphatase (BALP) and fibroblast growth factor (FGF-23), left ventricular mass index (LVMI), coronary artery calcification scores (CACS) before and after treatment and adverse reactions were compared between the two groups. Results: The effective rate of treatment in observation group was better than that in control group (P<0.05). Differences of serum Ca, P, iPTH, FGF-23 and ALP levels in observation group before and after treatment were larger than those in control group (P<0.05), while the differences of LVMI and CACS were smaller than those in control group (P<0.05). There was no significant difference in incidence of adverse reactions between the two groups (P>0.05). Conclusion: The curative effect of LC is good on MHD combined with hyperphosphatemia, which can improve CAC in patients. 2020 Vol. 26 (8): 1371-1375 [Abstract] ( 91 ) HTML (1 KB)  PDF (1197 KB)  ( 283 ) 1376 Expressions and Clinical Significance of Creatine Kinase and Lactate Dehydrogenase in Serum of Beonatal Bilirubin Encephalopathy CHEN Xiuxia, et al DOI: 10.3969/j.issn.1006-6233.2020.08.033 Objective: To investigate the expressions and clinical significance of creatine kinase (CK) and lactate dehydrogenase (LDH) in serum of neonatal bilirubin encephalopathy. Methods: 120 children with hyperbilirubinemia who were hospitalized in Qingdao Municipal Hospital from January 2017 to October 2019 were selected, among them, 67 children with bilirubin encephalopathy were in the case group and 53 children without bilirubin encephalopathy were in the control group. The levels of serum CK and LDH were measured by enzymology rate method; the level of serum total bilirubin (TBIL) was measured by caffeine azo colorimetry; Pearson correlation method was used to analyze the correlations between the levels of serum CK, LDH and level of serum TBIL, BAEP; and the related factors of neonatal bilirubin encephalopathy were analyzed. Results: The peak value of serum total bilirubin, the proportion of acidosis, the level of serum TBIL and BAEP in the case group were significantly higher than those in the control group (P<0.05); the levels of serum CK and LDH in the case group were significantly higher than those in the control group (P<0.05); the results of Pearson analysis showed that the levels of serum CK and LDH were positively correlated with level of serum TBIL and BAEP (P<0.001); and the results of logistic regression analysis showed that the main risk factors of bilirubin encephalopathy were high peak value of total bilirubin, acidosis, serum CK and LDH levels (P<0.05). Conclusion: The levels of CK and LDH in the serum of neonatal bilirubin encephalopathy are increased, which are certainly related to the level of serum TBIL and BAEP. They are risk factors of neonatal bilirubin encephalopathy and may be used as reference indexes to evaluate neonatal bilirubin encephalopathy. 2020 Vol. 26 (8): 1376-1379 [Abstract] ( 97 ) HTML (1 KB)  PDF (1195 KB)  ( 325 ) 1380 Analysis of Effect of Neoadjuvant Radiotherapy and Chemotherapy on Postoperative Complications and LARS in Patients with Locally Advanced Rectal Cancer CHAI Guangjin, XU Man, LI Weiwei, et al DOI: 10.3969/j.issn.1006-6233.2020.08.034 Objective: To study the incidence of postoperative complications and low-order anterior resection syndrome (LASRS) in patients with locally advanced rectal cancer. Methods: From March 2015 to June 2018, 115 patients with rectal cancer diagnosed in our hospital and treated with neoadjuvant radiotherapy and chemotherapy and surgery were divided into no LARS group and LARS group according to the situation of the patients. The postoperative complications of the two groups were analyzed, and the baseline data of the two groups were analyzed. The influencing factors of LARS were analyzed by univariate and multivariate Logistic regression analysis. Results: Of the 115 patients, 46 had Lars, 69 had no Lars. Univariate analysis showed that the time from the completion of operation and the position of anastomotic stoma were the suspicious related factors of Lars, and the difference was statistically significant (P<0.05). Multivariate logistic regression analysis showed that 3~6 months from the completion of the operation and less than 5cm from the anal margin were the related risk factors, and the difference was statistically significant (P<0.05). There was no significant difference in the incidence of other complications between patients with Lars and those without Lars (P>0.05). Conclusion: 3~6 months before operation and less than 5cm from anal margin were the influencing factors of Lars in locally advanced rectal cancer patients after neoadjuvant chemotherapy. There was no significant difference in the incidence of other complications between patients with Lars and those without Lars. 2020 Vol. 26 (8): 1380-1384 [Abstract] ( 135 ) HTML (1 KB)  PDF (1192 KB)  ( 447 ) 1385 Effects of Propofol Combined with Remifentanil on Hemodynamics and Brain Cell Function in Patients with Emergency Craniocerebral Injury GAO Sanjie, ZHANG Xianjie, XIA Leqiang, et al DOI: 10.3969/j.issn.1006-6233.2020.08.035 Objective: To investigate the effects of propofol combined with remifentanil on hemodynamics and brain cell function in patients with emergency craniocerebral injury. Methods: 64 patients with emergency craniocerebral injury surgery in our hospital from March 2018 to September 2019 were selected as the research objects. The subjects were randomly divided into study group and control group, 32 cases in each group. The control group used remifentanil anesthesia during the operation, and the study group used propofol and remifentanil anesthesia. The hemodynamic levels and brain cell function indexes of the two groups were observed and compared before operation (T0), 1 hour after anesthesia (T1), at the end of operation (T2), and 2 hours after operation (T3). Results: There were no significant differences in the levels of SBP, DBP, NSE and MBP between the two groups before surgery (P>0.05). At 1 h after anesthesia and at the end of the operation, the SBP and DBP of the two groups were lower than those before the operation, while the NSE and MBP levels were higher than the preoperative ones, and the difference was statistically significant (P<0.05). At 2 h after operation, the SBP, DBP, NSE, and MBP levels in the two groups were higher than those before surgery, 1 h after anesthesia, and at the end of surgery, and the differences were statistically significant (P<0.05). Conclusion: Propofol combined with remifentanil can effectively improve the hemodynamic level of patients with emergency craniocerebral injury and protect their brain cell function, which is beneficial to the patient's prognosis and is worthy of clinical application. 2020 Vol. 26 (8): 1385-1388 [Abstract] ( 104 ) HTML (1 KB)  PDF (1196 KB)  ( 244 ) 1389 Effects of High Flow Humidified Oxygen Therapy on the Expression Levels of CRP and PCT in Elderly Patients with Pneumonia WANG Guoyu, BA Chunhe, et al DOI: 10.3969/j.issn.1006-6233.2020.08.036 Objective: To compare the therapeutic effect of high flow humidified oxygen therapy and conventional oxygen therapy on pneumonia in elderly patients, and to provide basis for clinical application of high flow rate humidification oxygen therapy. Methods: 80 elderly patients with pneumonia who were admitted to our hospital from October 2016 to December 2019 were selected and randomly divided into the control group and the research group according to different treatment methods. Patients in the control group were treated with transnasal continuous positive pressure ventilation therapy, and patients in the research group were treated with high-flow humidified oxygen therapy. There were 40 cases in each group. The clinical efficacy and PCT, CRP, SpO2, RR, HR levels of the two groups before and after treatment were compared. Results: The comparison of clinical symptom indexes and PCT, CRP, SpO2, RR, HR levels between the two groups before treatment revealed no statistical differences(P>0.05).After treatment, the disappearance time of pulmonary rales, shortness of breath, expectoration and the length of hospital stay were shorter than the control group (P<0.05). PCT, CRP, RR, HR level of the research group were lower, and SpO2 level was higher, than the control group, and the difference was significant (P<0.05). Conclusion: The clinical effect of HFNC in the treatment of pneumonia in the elderly is significantly better than that of traditional oxygen therapy. 2020 Vol. 26 (8): 1389-1391 [Abstract] ( 85 ) HTML (1 KB)  PDF (1176 KB)  ( 306 ) 1392 The Effect of Hypotonic Contrast Agent Iohexol and Isoosmotic Contrast Agent Iodixanol on Renal Function in Enhanced CT LIU Yong, LING Xuefeng, CHEN Guozhang DOI: 10.3969/j.issn.1006-6233.2020.08.037 Objective: To compare the effect of iohexol, a hypotonic contrast agent, with iodixanol, an isoosmolar contrast agent, on the renal function of patients. Methods: 80 cases of enhanced CT in the radiology department of the People's Hospital of Haixi Autonomous Prefecture from March 2018 to December 2019 were analyzed retrospectively. According to the use of different contrast agents, they were divided into the observation group and the control group, 40 cases in each group. The renal function, contrast effect and adverse reactions of the two groups were compared before and after operation. Results: The levels of SCR, UAER and BUN in the observation group were (84.32±6.42) umol /L, (83.48±10.49) mg /24h, (4.90±1.12) mmol /L, respectively, which were significantly different from those in the control group (P<0.05); the contrast results showed that the excellent and good rate in the observation group was 95.0%, in comparison with 90.0% of the other group, and the difference was not statistically significant (P>0.05). The adverse reaction in one week follow-up was found in 2 patients in the observation group, accounting 5.0%, lower than 20.0% in the control group, with statistical significance (P<0.05). Conclusion: The application of icosahol in enhanced CT can reduce the influence on renal function of patients on the basis of ensuring the contrast effect, and has few adverse reactions, which is worthy of clinical reference. 2020 Vol. 26 (8): 1392-1394 [Abstract] ( 237 ) HTML (1 KB)  PDF (1169 KB)  ( 1054 ) 1395 A Study of Curative Effect of Split Nuclear Technique on Small Incision Cataract in Anterior Chamber Horizontal Space WU Zidong, CHEN Xuehong, ZHANG Hong, et al DOI: 10.3969/j.issn.1006-6233.2020.08.038 Objective: To investigate the curative effect of split nuclear technique on small incision cataract in anterior chamber horizontal space. Methods: The 110 patients with simple age-related cataract treated in our hospital were divided into control group and experimental group according to the patients' voluntary choice of surgical methods. 51 patients in the control group with 56 eyes underwent cataract phacoemulsification, and 59 patients in the experimental group with 68 eyes underwent small incision cataract. Correlative analysis was conducted of visual acuity, astigmatism, central corneal thickness, and complications before and after treatment in the horizontal space of the anterior chamber. Results: Before operation, the visual acuity of the control group and the experimental group were all less than 0.5, and it was improved in both groups compared with that at 1d, 1mo, and 3mo after surgery (P<0.05). And the control group and the experimental group were compared with each other at the same time. The difference was small (P>0.05). The difference of corneal astigmatism in the control group and the experimental group was small (P>0.05) before operation, and the difference was greater than that before surgery (P<0.05), and as to the rest time, there was no difference (P>0.05); the central corneal thickness of the control group and the experimental group was not significant compared with that before the operation, 1 day, 1mo, and 3mo after the operation (P>0.05). At different time points, the central corneal thickness ratio was significantly larger 1d after the operation, compared with the preoperative period (P<0.05), and in the remaining time it was smaller (P>0.05). Compared with the control group, the incidence of postoperative adverse events in the experimental group was reduced and there was a statistical difference. (8.82% vs 21.43% χ2=5.257, P<0.05). Conclusion: Clip splitting technique on small incision cataract in anterior chamber horizontal space can effectively improve the visual acuity, and it is safe and worthy of clinical application. 2020 Vol. 26 (8): 1395-1399 [Abstract] ( 104 ) HTML (1 KB)  PDF (1186 KB)  ( 270 ) 1400 An Analysis of Predictors of Intrauterine Balloon Tamponade Failure in Persistent Postpartum Hemorrhage After Vaginal Delivery ZHANG Zhaoju, TANG Gui'e DOI: 10.3969/j.issn.1006-6233.2020.08.039 Objective: To explore the influencing factors and predictive value of balloon tamponade failure in persistent postpartum hemorrhage after vaginal delivery. Methods: 80 cases of PPH patients after vaginal delivery, between September 2016 and September 2019, were retrospectively selected as the research subjects, and divided, by the outcomes of filling the balloon catheter, into success group (59 cases) and failure group (21 cases). The basic data and surgical indicators of the two groups were collected, Logistic analysis was conducted to the influencing factors, and the predictive value was analyzed by ROC curve. Results: Basic data comparison shows that in failure group had, than the success group, significantly higher occurrence of high maternal age, preeclampsia, scar uterus, placenta previa, placenta increta, weakness of contractions, diffuse intravascular coagulation, shock, blood transfusion and macrosomia (P<0.05). Surgical indicators showed that in terms of 24 h blood loss, balloon infusion volume and indwelling time, failure group had significantly higher figure than the success group (P<0.05). Logistic analysis showed that age, preeclampsia, blood loss, balloon infusion volume and indwelling time were all influencing factors for the failure of balloon catheter filling (P<0.05). ROC curve results showed that the age, preeclampsia, blood loss, balloon infusion volume, the indwelling time and the five joint AUC were 0.684, 0.791, 0.669, 0.850, 0.869, 0.940, and sensitivity were 0.797, 0.815, 0.763, 0.661, 0.814, 0.898, specific degrees were 0.524, 0.667, 0.619, 0.762, 0.857, 0.905. Conclusion: Age, preeclampsia, large blood loss, low balloon infusion volume and short indwelling time were all the reasons for failure of balloon compression hemostasis, and multiple combinations could improve the clinical prediction effect. 2020 Vol. 26 (8): 1400-1403 [Abstract] ( 121 ) HTML (1 KB)  PDF (1301 KB)  ( 441 ) 1404 An Analysis of the Association Between Serum Tumor Markers Levels and Pathological Features and Prognosis in Patients with Ovarian Cancer XING Huarui, FENG Yani, LI Xiaofang, et al DOI: 10.3969/j.issn.1006-6233.2020.08.040 Objective: To study the serum levels of human epididymis protein 4 (HE4), cancer antigen 125 (CA125) and chitinase protein 40 (YKL-40) in patients with ovarian cancer and their association with pathological features and prognosis. Methods: A total of 92 patients with ovarian masses who underwent surgery in Qionghai Hospital of traditional Chinese Medicine from January 2016 to August 2019 were included in the study. According to the results of surgical pathology, they were divided into benign group (n=52) and malignant group (n=40). The levels of serum HE4, CA125 and YKL-40 were compared between the two groups. The relationship between serum HE4, CA125 and YKL-40 levels and different pathological features was analyzed in patients with ovarian cancer. The survival curves of the subjects were plotted to analyze the association between postoperative serum HE4, CA125 and YKL-40 levels and prognosis of patients. Results: The serum levels of HE4, CA125 and YKL-40 in malignant group were significantly higher than those in benign group (P<0.05). The serum HE4 level in patients with ovarian cancer was correlated with pathological tissue types and lymphatic metastasis (P<0.05), and serum CA125 was correlated with operation staging, tissue types, lymphatic metastasis and age (P<0.05), and serum YKL-40 was correlated with operation staging and lymphatic metastasis (P<0.05). The serum levels of HE4, CA125 and YKL-40 after operation in patients with ovarian cancer were significantly lower than those before operation (P<0.001). According to the median method, patients with ovarian cancer were divided into postoperative serum HE4 high-level group (n=17) and low-level group (n=23), CA125 high-level group (n=15) and low-level group (n=25), YKL-49 high-level group (n=22) and low-level group (n=18). The survival curves of the subjects found there was no significant difference in the survival curve of patients with postoperative serum HE4 high-level and low-level (χ2=2.42, P>0.05), and there was a significant difference in the survival curve of patients with serum CA125 high-level and low-level (χ2=4.12, P<0.05), and there was no significant difference in the survival curve of patients with serm YKL-40 high-level and low-level (χ2=1.94, P>0.05). Conclusion: The serum levels of HE4, YKL-40 and CA125 in patients with ovarian cancer can indicate the condition and prognosis of patients to some extent. 2020 Vol. 26 (8): 1404-1408 [Abstract] ( 102 ) HTML (1 KB)  PDF (6499 KB)  ( 376 )

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