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2020 Vol. 26, No. 7 Published: 2020-07-31 1057 Effect of Tanshinone IIA on Hyperalgesia Induced by Vincristine in Rats with Neuropathic Pain and its Mechanism FU Baojun, JIANG Jingjing, HUANG Yuqiong, et al DOI: 10.3969/j.issn.1006-6233.2020.07.001 Objective: To investigate the effect of intraperitoneal tanshinone IIA on hyperalgesia induced by vincristine in rats with neuropathic pain and its possible mechanism. Methods: A total of 40 healthy male SD rats were randomly divided into 4 groups (10 in each): control group, chemotherapy-induced neuropathic pain (CINP) group (modeled by intraperitoneal injection of vincristine every other day), CINP+NS group (Successful establishment of CINP model and intraperitoneal injection of normal saline), CINP +Tan group (Successful establishment of CINP model and intraperitoneal injection of tanshinone IIA). Mechanical allodynia and heat hyperalgesia was evaluated with thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT). Western blotting was used to detect the expression of phosphorylated c-Jun N-terminal kinase (p-JNK), glial fibrillary acidic protein (GFAP) and Ionized calcium binding adapter molecule (Iba1) in spinal cord, immunofluorescence assay to detect the Co-localization expression of GFAP and p-JNK, reverse transcription-polymerase chain reaction to detect the mRNA expression of tumor Necrosis Factor-α (TNF- α), interleukin- 6 (IL-6) and interleukin-1 (IL-1β) andenzyme-linked immunosorbent assay to detect the protein expression of TNF- α, IL-6 and IL-1β. Results: The TWL and MWT in group CINP were significantly lower those in the control group from 3 days to 14 days after the first injection of vincristine (P<0.01). The MWT and TWL in group CINP+Tan were significantly higher than those in group CINP +NS (P<0.01). Significant up- regulation was found in group CINP as compared with the control group in protein expression of p-JNK, GFAP, TNF- α, IL-6 and IL-1β and the Mrna expression of TNF- α, IL-6 and IL-1β in spinal cord (P<0.01). Significant down-regulation was observed in group CINP+Tan as compared with the CINP+NS group in the protein expression of p-JNK, GFAP, TNF- α, IL-6, IL-1β and the mRNA expression of TNF- α, IL-6 and IL-1β in the spinal cord (P<0.05). The co-localization expression of GFAP and p-JNK were observed in spinal cord. Conclusion: Tanshinone IIA can inhibit the hyperalgesia of neuropathic pain rats induced by vincristine, and its mechanism may be related to the activation of JNK pathway and inflammation of astrocytes. 2020 Vol. 26 (7): 1057-1061 [Abstract] ( 184 ) HTML (1 KB)  PDF (1604 KB)  ( 405 ) 1062 Clinical Observation of Laparoscopic Cholecystectomy and Mini-incision Cholecystectomy for Cholelithiasis and its Effect on Immune Function FANG Jian, KAI Zhe, CHEN Zhuming DOI: 10.3969/j.issn.1006-6233.2020.07.002 Objective: To explore the clinical observation of laparoscopic cholecystectomy and mini-incision cholecystectomy for cholelithiasis and its effect on immune function. Methods: 104 patients with cholecystolithiasis undergoing elective surgery in general surgery of our hospital between October 2017 and April 2019 were divided into the test group(n=52) and the control group(n=52) by simple random grouping method. Patients in the control group were treated with mini-incision cholecystectomy, while patients in the test group were treated with laparoscopic cholecystectomy. The operation conditions, postoperative complications and T lymphocyte subsets between the two groups were compared. Results: The total effective rate of the test group was significantly higher than that of the control group (94.2% vs 76.9%, P<0.05). The incidence of postoperative complications in the test group was lower than that in the control group (11.5% vs. 26.9%, P<0.05). The total incision length, operation time, postoperative hospitalization time, anal exhaust time and ambulation time of the taet group were shorter than those of the control group, and the intraoperative blood loss was less than that of the control group (P<0.05). The levels of CD3+T, CD4+T, CD4+T/CD8+T in the test group were higher than those in the control group (P<0.05). Conclusion: Laparoscopic cholecystectomy with smaller incision cholecystectomy has better therapeutic effect on patients with gallstone, less immunosuppression and better postoperative recovery. 2020 Vol. 26 (7): 1062-1065 [Abstract] ( 146 ) HTML (1 KB)  PDF (1217 KB)  ( 225 ) 1066 Effects of Etomidate on PI3K / AKT Pathway and Apoptosis of Non-small Cell Lung Cancer A549 Cells DU Jia'nan, et al DOI: 10.3969/j.issn.1006-6233.2020.07.003 Objective: To investigate the effect of etomidate on PI3K/AKT pathway and apoptosis of non-small cell lung cancer A549 cell. Methods: A549 cell group, 5-fluorouracil group, etomidate low-dose group and high-dose group were set up. 5-fluorouracil group, etomidate low-dose group and high-dose group were added 5-fluorouracil group and etomidate at different concentrations, and the final concentration was 100.0ug/mL, 100.0ug/mL and 200.0ug/mL for 72 hours. Cell proliferation level was assessed by cell counting kit-8, apoptotic level was measured by flow cytometry, cell invasiveness was measured by Transwell chamber, p-PI3K, p-AKT mRNA and protein levels were measured by RT-PCR and WEST-BLOT. Results: Compared with A549 cell group, the OD value, survival rate, number of perforation, p-PI3K, p-AKT gene and protein were significantly decreased in 5-fluorouracil group, low-dose and high-dose etomidate group(P<0.05), apoptotic rate were significantly increased(P<0.05). And with the increase of etomidate dosage, the OD value, survival rate, number of perforation, p-PI3K, p-AKT gene and protein were gradually decreased (P<0.05), apoptotic rate were gradually increased (P<0.05). Compared with 5-fluorouracil group, the OD value, survival rate, number of perforations, p-PI3K, p-AKT mRNA and protein in low-dose etomidate group were significantly increased(P<0.05), and apoptotic rate were significantly decreased (P<0.05); while the OD value, survival rate, number of perforation, p-PI3K, p-AKT mRNA and protein in high-dose etomidate group were significantly decreased(P<0.05), and apoptotic rate were significantly increased(P<0.05). Conclusion: Etomidate can inhibit the proliferation and invasion of lung cancer A549 cells and promote the apoptosis of lung cancer A549 cells. Its mechanism is related to the inhibition of PI3K/AKT pathway activation and transmission by reducing the levels of p-PI3K, p-AKT mRNA and protein phosphorylation by etomidate. 2020 Vol. 26 (7): 1066-1071 [Abstract] ( 165 ) HTML (1 KB)  PDF (1479 KB)  ( 299 ) 1072 The Predictive Value of the Ratio of Neutrophils to Lymphocytes in Patients with Acute Cerebral Infarction CAO Xiaofeng, WU Minghua DOI: 10.3969/j.issn.1006-6233.2020.07.004 Objective: To explore the predictive value of peripheral blood neutrophil lymphocyte ratio (NLR) in the poor prognosis of patients with acute cerebral infarction. Methods: 660 patients with acute cerebral infarction (onset<72h) from January 2017 to May 2018 in the center for encephalopathy of Jiangsu Hospital of Chinese medicine were retrospectively included. The ability of NLR to predict the unfavorable prognosis of 90 days was tested by using the ROC curve, and the survival of patients was analyzed by Kaplan Meier survival curve. Results: NLR had a certain value in predicting the poor prognosis of patients with acute cerebral infarction. Kaplan Meier survival analysis showed that the survival rate of patients with NLR > 3.23 was significantly lower than that of patients with NRL ≤ 3.23 (log rank P = 0.000). Conclusion: The higher level of NLR has a certain predictive value for predicting the short-term survival of ACI patients in the early stage, and is related to the higher risk of death at 90 days. 2020 Vol. 26 (7): 1072-1074 [Abstract] ( 154 ) HTML (1 KB)  PDF (1264 KB)  ( 311 ) 1075 The Effects of Thoracoscopy and Thoracotomy on Qs/Qt Inflammatory Factors and Lymph Node Clearance During Perioperative One-lung Ventilation in Patients with Esophageal Cancer ZHANG Biao, LI Junqing, GU Jiangkui DOI: 10.3969/j.issn.1006-6233.2020.07.005 Objective: To investigate the effects of thoracoscopy and thoracotomy on Qs/Qt, inflammatory factors and lymph node dissection during perioperative one-lung ventilation in patients with esophageal cancer. Methods: 58 cases of esophageal carcinoma between July 2017 and October 2019 in our hospital were selected prospectively as the research object and divided on the patients’ own willingness into traditional thoracotomy group (the control group, 26 cases) and thoracoscope group (observation group,32 cases). Clinical indicators were collected, Qs/Qt at different time points of single lung ventilation was recorded, inflammatory factor levels were analyzed, and prognosis was observed. Results: Postoperative Qs/Qt levels of 2 groups of patients were significantly elevated at each point of time, and the difference was statistically significant (P<0.05); The same point of time comparison showed that in the observation group Qs/Qt levels of T1, T2 and T3 were (37.28±1.76) %, (44.91±2.57) %, (38.82±3.67) %, significantly higher than those of the control group (30.31±1.80) %, (37.60±3.01) %, (33.28±3.15) %, and the difference was statistically significant (P<0.05). The comparison of clinical indexes showed that blood loss and hospital stay of the observation group were (186.66±71.25)mL, (12.85±2.06)d, significantly lower than the control group (352.93±80.99) mL, (14.46±2.25) d, and the difference was statistically significant (P<0.05). Postoperative comparison showed that 2 d TNF-α, IL-8 levels in preoperative period and postoperative 2 d period were (37.59±11.31)pg/mL, (132.66±34.9) pg/mL, (13.98 ± 5.22)pg/mL, (91.27±28.96) pg/mL, significantly lower than control group (56.83±16.64) pg/mL, (189.63±56.86)pg/mL, (32.60±6.57) pg/mL, (161.28±34.65) pg/mL, and the difference was statistically significant (P<0.05). In addition, the operation time, the number of lymph nodes clearance, the incidence of complications and survival time had no statistically significant difference (P>0.05). Conclusion: Both thoracoscopy and laparotomy had similar effects on lymph node clearance, complications and survival time of esophageal cancer, but thoracoscopy had more advantages in shortening hospital stay and reducing inflammatory response, while the Qs/Qt level of single-lung ventilation during thoracotomy was lower. 2020 Vol. 26 (7): 1075-1078 [Abstract] ( 116 ) HTML (1 KB)  PDF (1315 KB)  ( 246 ) 1079 The Effect of Argatroban on Neurological Deficit Score Barthel Index and Hemorheology in Patients with Posterior Circulation Cerebral Infarction LIU Chunyu, WU Jianhong, YANG Xiaodan DOI: 10.3969/j.issn.1006-6233.2020.07.006 Objective: To analyze the effect of argatroban on hemorheology, neurological deficit and activity of daily life in patients with posterior circulation cerebral infarction. Methods: A total of 120 patients with posterior circulation cerebral infarction admitted to our hospital from September 2017 to September 2019 were randomly divided into two groups by simple random grouping method: the control group was treated in conventional manner and the treatment group was administered argatroban + conventional. The hemorheology indexes, National Institutes of Health Stroke Scale (NIHSS) and the modified Barthel Index (MBI) of the two groups were compared before and after treatment. During the treatment, thromboelastography and coagulation function were performed in the treatment group to evaluate the effect of agatraban on the improvement of coagulation function. Results: Three months after treatment, Hct and whole blood relative shear rate index (high shear) in the control group were significantly reduced compared with corresponding data before treatment (P< 0.05), but there was no significant change in plasma viscosity and the shear rate of whole blood viscosity (high shear). The Hct, plasma viscosity, whole blood relative shear rate index (high shear) and shear rate of whole blood viscosity (high shear) were significantly reduced compared with corresponding data before treatment, and each index after treatment was significantly reduced compared with control group (P< 0.05). The NIHSS scores of the two groups decreased significantly after 3 months of treatment, and the decrease range of the treatment group after treatment was more significant compared with control group (P< 0.05); compared with corresponding data before treatment, the MBI scores of the two groups after 3 months of treatment were significantly increased, and the increase range of the treatment group after treatment was more significant compared with control group (P< 0.05). The prothrombin time, prothrombin time and activated partial thromboplastin time (APTT) in the treatment group were significantly prolonged compared with control group at 60 mg / d, while the prothrombin time, prothrombin time and APTT were significantly shorter at 30 mg / d and 2 d after stopping treatment. The comparison of coagulation function indexes at different time points was statistically significant (P< 0.05). After 60 mg / d, the coagulation index and the activity of coagulation factors in the treatment group decreased significantly, while after 30 mg / d and 2 days of withdrawal, the coagulation index and the activity of coagulation factors returned to the normal range (P< 0.05). Conclusion: The application of argatroban can effectively improve the hemorheology and coagulation function of patients with post circulation cerebral infarction, reduce the degree of neurological deficit, improve the ability of daily life activities, and has good safety, so it is worth clinical application. 2020 Vol. 26 (7): 1079-1083 [Abstract] ( 164 ) HTML (1 KB)  PDF (1249 KB)  ( 407 ) 1084 The Effect of Carotid CTA and Ultrasound in Arterial Plaque and Stenosis Diagnosis and the Imaging Feature LI Hanjian, CHEN Chunmei, XU Shan, et al DOI: 10.3969/j.issn.1006-6233.2020.07.007 Objective: To investigate the diagnostic effect and imaging features of carotid CTA and ultrasound in patients with arterial plaque and stenosis. Methods: Eighty patients with carotid atherosclerosis and stenosis were enrolled in our hospital from September 2018 to September 2019. All patients underwent carotid ultrasound and CTA so as to determine the accuracy of plaque and stenosis, as well as its imaging characteristics. Results: There were 160 carotid arteries in 80 patients. The diagnostic yields of the two methods were both 68.75% and the agreement was 100%. There was no significant difference between the two methods in the classification of soft plaques and mixed plaques (P> 0.05), and the difference in calcified plaque was statistically significant (P＜0.05); the diagnostic yields of carotid ultrasound and CTA for vulnerable plaque was 50.00% and 36.36%, respectively, and the difference was statistically significant (P＜0.05); There was no significant difference in the classification of carotid stenosis between the two methods (P>0.05). Conclusion: Carotid ultrasound and CTA have advantages in the nature and extent of carotid plaque. Reasonable application of two detection methods can improve the diagnostic accordance rate. 2020 Vol. 26 (7): 1084-1087 [Abstract] ( 159 ) HTML (1 KB)  PDF (1501 KB)  ( 692 ) 1088 The Curative Effect of Cilazapril Combined with Isosorbide Mononitrate on COPD and Heart Failure and The Influences on Serum Cardiopulmonary Function Indexes and Inflammatory Factors LIN Dandan, ZHOU Yan, et al DOI: 10.3969/j.issn.1006-6233.2020.07.008 Objective: To explore clinical curative effect of cilazapril combined with isosorbide mononitrate on COPD and heart failure and their influences on serum cardiopulmonary function and inflammatory factors. Methods: A total of 150 patients with COPD and heart failure who were admitted to the hospital from May 2017 to March 2018 were enrolled. They were divided into observation group (n=75) and control group (n=75) by simple random grouping method. The control group was treated with isosorbide mononitrate, while observation group was additionally treated with cilazapril. The clinical curative effect, cardiopulmonary function and inflammatory factors indexes were compared between the two groups. Result: The total response rate of clinical curative effect in observation group was higher than that in control group (90.67% vs 78.67%) (P<0.05). Before treatment, there were no statistical differences in cardiac function, pulmonary function and inflammatory factors indexes between the two groups (P>0.05). After treatment, levels of LVEF, FVC, FEV1 and FEV1/FVC in observation group were higher than those in control group (P<0.05), while levels of LVEDD, BNP, hs-CRP, serum IL-6, IL-8 and TNF-α were lower than those in control group (P<0.05). Conclusion: The curative effect of cilazapril combined with isosorbide mononitrate is significant on COPD combined with heart failure. It can improve response rate of treatment, clinical symptoms and cardiopulmonary function, down-regulate expression of inflammatory factors, and reduce inflammatory response. 2020 Vol. 26 (7): 1088-1091 [Abstract] ( 135 ) HTML (1 KB)  PDF (1198 KB)  ( 270 ) 1092 The Effects and Safety of Different Treatments on Serum β-hCG P and VEGF in Patients with Ectopic Pregnancy LI Fan, WANG Ruping, ZHOU Ping DOI: 10.3969/j.issn.1006-6233.2020.07.009 Objective: To explore the effects of conservative medication, open surgery and laparoscopic surgery on patients with ectopic pregnancy. Methods: The clinical data of 80 patients with ectopic pregnancy admitted to the hospital from March 2016 to March 2019 were retrospectively analyzed. According to different treatment methods, they were divided into conservative medication group (mifepristone + methotrexate + Shapei prescription, 30 cases), open group (openfallopian tube resection, 30 cases) and laparoscopic group (laparoscopic fallopian tube resection, 20 cases). At 1 week after treatment, the levels of serum β-human chorionic gonadotropin (β-hCG), progesterone (P) and vascular endothelial growth factor (VEGF) were compared among the three groups, and the clinical efficacy was evaluated by serum indexes and imaging results. The adverse reactions or complications were monitored in the three groups after treatment. The hospital stay and hospitalization expense were compared among the three groups. At 3 months after treatment, the psychological states of the patients were evaluated by Self-rating Anxiety Scale (SAS) and Self-rating Depression Scale (SDS), and the sexual function was evaluated by Female Sexual Function Index (FSFI). Results: At 1 week after treatment, the levels of serum β-hCG, P and VEGF in open group and laparoscopic group were significantly lower than those in conservative medication group (P<0.05), but there were no significant differences between open group and laparoscopic group (P>0.05). The efficacy was 100.00% in open group and laparoscopic group, which was significantly higher than that in conservative medication group with 63.33% (P<0.05). There were no significant differences in the incidence rates of adverse reactions or complications among the three groups (P>0.05). The hospital stay in conservative medication group was significantly longer than that in open group and laparoscopic group (P<0.05), and the hospitalization expense was significantly lower than that in open group and laparoscopic group (P<0.05). At 3 months after treatment, the reductions of SAS and SDS scores in conservative medication group were greater than those in open group (P<0.05) while the decrease of FSFI score was smaller than that in open group (P<0.05), but there were no significant differences compared with those in laparoscopic group (P>0.05). Conclusion: Surgical treatment of patients with ectopic pregnancy can improve serum β-hCG, P and VEGF faster, and its early efficacy is better than conservative treatment, but conservative treatment is superior to surgical treatment in hospitalization expense and psychological states and sexual function status after treatment. And surgical treatment medication have better safety, and it is necessary to take individualized treatment by combining with the actual situation in clinical practice. 2020 Vol. 26 (7): 1092-1096 [Abstract] ( 108 ) HTML (1 KB)  PDF (1237 KB)  ( 274 ) 1097 An Analysis of the Role of Fecal SFRP2 Gene Methylation Combined with Quantitative Immunochemical Fecal Occult Blood Test in Colorectal Cancer Screening KANG Qian, XIE Hui, WANG Xin, et al DOI: 10.3969/j.issn.1006-6233.2020.07.010 Objective: To investigate the clinical significance of SFRP2 gene methylation combined with quantitative immunochemical fecal occult blood test for colorectal cancer screening. Methods: 106 patients with colorectal cancer,106 patients with advanced adenoma and 106 healthy people who underwent colonoscopy and pathology in the hospital's gastroenterology clinic were selected. Fecal samples were collected for Methylation-specific PCR to detect the methylation of fecal SFRP2 gene promoter and quantitative immunochemical fecal occult blood. Positive rate was calculated. Results: The positive rate of fecal SFRP2 gene methylation and positive QFIT were 77.35%,25.47%, 6.60%, 83.96%,15.09%, 1.87% in the colorectal cancer group, advanced adenoma group and control group, respectively. The cancer group and the advanced adenoma group were higher than the control group (P<0.05), and the colorectal cancer group was higher than the advanced adenoma group (P<0.05). The positive rate of SFRP2 methylation combined with QFIT was 91.51%, 52.83% and 5.66% in the colorectal cancer group, advanced adenoma group and control group, respectively. The detection rate of colorectal cancer group and the advanced adenoma group were higher than the individual test. (P<0.05). SFRP2 gene methylation in feces, the sensitivity of fecal occult blood quantification and combined detection were 77.35%, 83.96% and 93.40%,respectively, and the specificities were 93.40%, 98.11% and 93.40%, respectively. The sensitivity of the combined detection were higher than individual test (P<0.05). Conclusion: SFRP2 methylation combined with fecal occult blood quantification could improve the accuracy of screening colorectal cancer and have potential clinical value. 2020 Vol. 26 (7): 1097-1099 [Abstract] ( 241 ) HTML (1 KB)  PDF (1206 KB)  ( 511 ) 1100 An Observation of the Effect of Zirconia on Mastication Ability and Its Application in Oral Repair LI Yingying, et al DOI: 10.3969/j.issn.1006-6233.2020.07.011 Objective: To observe the effect of zirconia on the mastication ability and the restorative effect. Methods: 96 patients were randomly divided into observation group and control group, 48 patients in each group. Patients in the control group were treated with ni-cr alloy prosthesis and patients in the observation group were treated with zirconia prosthesis. The repair effect of the two groups was observed, as well as the changes of chewing ability, gingival index, IL-6 and IL-8 in gingival crevicular fluid before and after repair, and the repair quality. Results: After treatment, the bite force and chewing ability of the two groups were improved, and the improvement effect of the observation group was better, and the difference between the groups was statistically significant (P<0.05).The high quality rate of treatment in the observation group was better than that in the control group, and the difference between the groups was statistically significant (P<0.05).After treatment, gingival index, bleeding index and plaque index were improved in both groups, and the improvement effect was better in the observation group, with statistically significant difference between groups (P<0.05).The levels of IL-6 and IL-8 in gingival crevicular fluid in the observation group after repair were lower than those in the control group, and the difference between groups was statistically significant (P<0.05). In the observation group, the rate of edge tightness was higher than that of the control group, and the fracture rate of the restoration body and porcelain breakage rate of the restoration body were lower than that of the control group, with statistically significant difference (P<0.05). Conclusion: the application of zirconia prosthesis in the treatment of patients with oral prosthesis has a good effect, which can significantly improve the chewing ability of patients and improve the gingival function. 2020 Vol. 26 (7): 1100-1103 [Abstract] ( 112 ) HTML (1 KB)  PDF (1209 KB)  ( 347 ) 1104 Effects of Sequential Chemoradiotherapy on Curative Effect of Postoperative Adjuvant Therapy for Patients with Advanced Endometrial Cancer and Levels of Serum HE4 CA125 GUO Wei, DIAO Haidan DOI: 10.3969/j.issn.1006-6233.2020.07.012 Objective: To explore effects of sequential chemoradiotherapy on curative effect of postoperative adjuvant therapy for patients with advanced endometrial cancer (EC) and levels of serum HE4, CA125. Methods: The clinical data of 80 patients with advanced endometrial cancer from October 2015 to October 2017 were analyzed retrospectively, According to different treatment methods, they were divided into control group (n=40) and observation group (n=40). The control group was given routine adjuvant chemoradiotherapy, while observation group was given sequential chemoradiotherapy. The clinical curative effect, serum carbohydrate antigen 125 (CA125), human epididymis protein (HE4), incidence of complications, tumor metastasis rate, survival rate and survival time were compared between the two groups. Results: After treatment, total response rate of observation group was higher than that of control group (P<0.05). After treatment, levels of serum HE4 and CA125 in observation group were lower than those in control group (P<0.05). The incidence of complications in observation group was lower than that in control group (P<0.05). The tumor metastasis rate in observation group was lower than that in control group (P<0.05).The patients were followed up for 2 year. The 2-year survival rate of observation group was higher than that of control group, and survival time was better than that of control group (P<0.05). Conclusion: The effects of sequential chemoradiotherapy are good on curative effect of postoperative adjuvant therapy for patients with advanced EC. It can reduce levels of serum HE4 and CA125 level, reduce incidence of complications and tumor metastasis rate, and improve quality of life. 2020 Vol. 26 (7): 1104-1106 [Abstract] ( 100 ) HTML (1 KB)  PDF (1220 KB)  ( 305 ) 1107 Diagnostic Efficacy of miRNA-192-5p in Multiple Myeloma and Its Correlation with Clinical Parameters GAN Dingyun, WU Jun, ZHOU Man DOI: 10.3969/j.issn.1006-6233.2020.07.013 Objective: To analyze the diagnostic efficacy of mirna-192-5p in multiple myeloma and the relationship between mirna-192-5p and clinical parameters. Methods: From May 2016 to may 2019, 90 patients with multiple myeloma treated in our hematology and oncology department were selected as the myeloma group, and 60 normal control group as the healthy people who came to our hospital for physical examination at the same time. The content of mirna-192-5p in the serum of patients with multiple myeloma and normal people was detected by fluorescence quantitative PCR, and the content of mirna-192-5p in the serum of multiple myeloma was analyzed by ROC curve. The correlation between mirna-192-5p and clinical parameters in patients with multiple myeloma was analyzed by Pearson correlation. Results: The expression level of mirna-192-5p was 0.72 ± 0.11 in the serum of patients with multiple myeloma and 1.08 ± 0.15 in the serum of normal control group, with statistical difference between the two groups (P<0.05); The analysis of ROC curve showed that the area under the curve of mirna-192-5p in the diagnosis of multiple myeloma was 0.653, the cutoff value is 0.68, the sensitivity was 77%, the specificity was 56%, P = 0.023; in β2-MG The content of mirna-192-5p in the serum of patients with high G content was significantly lower than that of patients with normal β 2-mg content (P<0.05). The content of mirna-192-5p in the serum of patients with high creatinine level was also significantly lower than that of patients with normal creatinine level (P<0.05). The content of mirna-192-5p in the serum of patients with low hemoglobin and albumin was significantly lower than that of patients with normal level (P<0.05). The content of mirna-192-5p was negatively correlated with the content of IgG and IgA respectively (r = -0.457, - 0.615, P<0.05). Conclusion: Mirna-192-5p has abnormal low expression in the serum of patients with multiple myeloma. It can only be used as an auxiliary diagnostic standard in the diagnosis of multiple myeloma. It has a certain correlation with disease stage and prognosis related indicators. It is a good marker in the progress monitoring of multiple myeloma. 2020 Vol. 26 (7): 1107-1110 [Abstract] ( 68 ) HTML (1 KB)  PDF (1389 KB)  ( 318 ) 1111 The Diagnostic and Therapeutic Values of Fungal D-glucan Detection in COPD Patients with Invasive Pulmonary Fungal Infection ZHOU Anwen, YUAN Hongxia DOI: 10.3969/j.issn.1006-6233.2020.07.014 Objective: To explore the diagnostic and therapeutic value of fungal d-glucan test (G test) for invasive pulmonary fungal infection (IPFI) in patients with chronic obstructive pulmonary disease (COPD). Methods: 200 patients with COPD diagnosed as IPFI from June 2017 to June 2019 in our hospital were selected as the research object. With fungal culture and smear positive results taken as the gold standard, all of the patients took G test, and sensitivity and specificity were calculated before analyzing the D-glucan content in valid and invalid cases. Results: Among the 200 patients to be diagnosed with IPFI, the results of fungal culture and smear were taken as the standard, and a total of 142 positive IPFI patients and 58 negative IPFI patients were detected. A total of 132 patients with positive IPFI and 68 patients with negative IPFI were detected by G test. The ROC curve showed that the AUC of G test for the diagnosis of COPD patients with IPFI was 0.904, the sensitivity was 89.43%, the specificity was 91.38%, and the accuracy was high. During the course of treatment, 118 patients (83.10%) were treated effectively, while 24 patients (16.90%) were not treated effectively. Further measurement of G test value showed no statistically significant difference between the two groups before treatment (P>0.05). After 1 and 2 weeks of treatment, G test value of the effective group significantly decreased, with statistically significant difference (P<0.05). Conclusion: The accuracy of plasma G test in diagnosing fungal bloodstream infection is relatively high, which has a good positive predictive value for the early diagnosis of fungal bloodstream infection, and has certain clinical guiding significance for predicting the treatment effect of patients. 2020 Vol. 26 (7): 1111-1114 [Abstract] ( 132 ) HTML (1 KB)  PDF (1314 KB)  ( 409 ) 1115 Research on the Effect of Ultrasound-guided Peripheral Nerve Block Anesthesia on Cognitive Function Stress Response and Outcome in Elderly Patients Undergoing Hip Replacement ZHONG Changyun, ZHAO Tianlan DOI: 10.3969/j.issn.1006-6233.2020.07.015 Objective: To analyze the effect of ultrasound-guided peripheral nerve block anesthesia on cognitive function, stress response and postoperative outcome in elderly patients undergoing hip replacement. Methods: 92 patients undergoing hip replacement in our hospital were randomly divided into control group and experimental group, 46 cases in each group. The control group was given general anesthesia, and the experimental group was given peripheral nerve block anesthesia under the guidance of ultrasound. The cognitive function of patients after operation was investigated by MMSE. The changes of interleukin-6 (IL-6) and cortisol (COR) levels at different stages were observed to evaluate the stress response. The outcome was evaluated by the dosage of intravenous controlled analgesia (PCIA), the time of getting out of bed, the time of extubation and the length of hospital stay. The adverse symptoms after operation were investigated. Results: The results showed that the MMSE scores of the experimental group was higher than those of the control group at 6 hours and 1 day after operation, and the difference was significant (P<0.05); the dosage of PCIA, time of getting out of bed, time of extubation and length of hospital stay in the experimental group was lower than those in the control group, and the difference was significant (P<0.05); the levels of IL-6 and COR in the first and fourth stages was lower than those in the control group, and the difference was significant(P<0.05); the incidence of adverse symptoms such as headache, vomiting and urinary retention in the experimental group was not significantly different from those in the control group (P>0.05). Conclusion: Ultrasound-guided peripheral nerve block anesthesia has little influence on cognitive function of elderly hip replacement patients, reduces stress response, avoids adverse symptoms, speeds up the outcome of surgery, has obvious analgesic effect and high application value. 2020 Vol. 26 (7): 1115-1118 [Abstract] ( 90 ) HTML (1 KB)  PDF (1221 KB)  ( 429 ) 1119 Comparison of the Diagnostic Value of Three-dimensional Ultrasound Mammography Combined with Full Field Digital Mammography and DBT Alone in Breast Cancer Diagnosis ZHAO Peng, XIAO Yang, LI Hongrui DOI: 10.3969/j.issn.1006-6233.2020.07.016 Objective: To explore the value of three-dimensional ultrasound mammography (DBT) combined with full field digital mammography (FFDM) and single DBT in the diagnosis of breast cancer, so as to provide theoretical and practical basis for the clinical diagnosis of breast cancer. Methods: Based on the gold standard of pathological diagnosis, 300 cases of breast cancer (all female) admitted by Harrison International Peace Hospital from October 2017 to December 2018 were selected for prospective study. FFDM and DBT images were obtained by combo mode on the basis of accurately obtaining breast density type, focus size, calcification focus and tissue type of all subjects. The images of DBT diagnosis were judged, and their data were included in spss19.0 and medcalc12.0 software processing. The sensitivity, specificity and accuracy of the two diagnostic methods and the diagnostic accuracy of evaluation (ROC curve of working characteristics of subjects) were analyzed. Results: 1) By statistical analysis, the results of DBT+FFDM in group A, B, C and D showed no significant difference (P＞0.05) compared with that in group A (ACR1, ACR2) and group B (< 10 mm and > 30 mm), and the results of DBT + FFDM in other indicators were significantly better than that of DBT alone (P＜0.05). 2). Statistical results showed that there was no significant difference between the DBT+FFDM detection of patients under 40 years old, over 50 years old and postmenopausal patients and that of patients with simple DBT diagnosis (P＞0.05), while the DBT+FFDM detection of patients between 40-50 years old and postmenopausal patients was significantly better than that of patients with simple DBT diagnosis (P＜0.05). 3). The AUC of DBT+FFDM was 0.937, 95% CI was 0.878~1.024, while that of DBT+FFDM was 0.781, 95% CI was 0.715~0.843, which was more accurate than that of DBT+FFDM (P＜0.05). Conclusion: In the clinical diagnosis of breast cancer, the diagnostic accuracy of DBT combined with FFDM is higher than that of DBT alone, which can be better used in clinical detection. It is the first choice for breast cancer examination and diagnosis of related breast diseases. It can provide more accurate image diagnosis results for clinical diagnosis and treatment of the disease, shorten the detection time of patients and reduce the clinical diagnosis cost. It has good clinical application value. 2020 Vol. 26 (7): 1119-1124 [Abstract] ( 121 ) HTML (1 KB)  PDF (1423 KB)  ( 402 ) 1125 Comparison of Postoperative Urinary Control Between Prostate Cancer Radical Operation with da Vinci Robot and Laparoscopic Prostate Cancer Radical Operation XU Ting, CAO Dehong, CHEN Ye, et al DOI: 10.3969/j.issn.1006-6233.2020.07.017 Objective: To compare curative effect, postoperative urinary control and safety between prostate cancer (PCa) radical operation with da Vinci robot and laparoscopic PCa radical operation, and to provide reference for developing safe and effective surgical procedures for PCa patients. Methods: A total of 255 PCa patients who were admitted to the hospital from July 2018 to January 2019 were enrolled, and divided into laparoscopic group (laparoscopic PCa radical operation, n=170) and robot group (PCa radical operation with da Vinci robot, n=85). The operation time, intraoperative blood loss, recovery time of intestinal function, postoperative hospital stay and treatment cost were compared between the two groups. The urinary control status in both groups at 1 month, 6 months and 12 months after surgery was followed up. The incidence of complications was compared between the two groups. Before surgery, at 6 months and 12 months after surgery, quality of life in both groups was evaluated by expanded prostate cancer index composite (EPIC). Results: Compared with laparoscopic group, operation time, recovery time of intestinal function and postoperative hospital stay were significantly shorter in robot group, intraoperative blood loss was significantly less, while treatment cost was significantly higher (P<0.05). At 1 month and 6 months after surgery, urinary control rate in robot group was significantly higher than that in laparoscopic group (P<0.05). At 12 months after surgery, there was no significant difference in urinary control rate between the two groups (P>0.05). At 6 months and 12 months after surgery, there were significant differences in quality of life scores between the two groups (P<0.05). At 6 months and 12 months after surgery, total score of quality of life in robot group was significantly higher than that in laparoscopic group (P<0.05). The incidence of complications in robot group was significantly lower than that in laparoscopic group (P<0.05). Conclusion: Compared with laparoscopic PCa radical operation, PCa radical operation with da Vinci robot in treatment of PCa is of significant advantages in terms of promoting rehabilitation process, improving urinary control rate, improving prognosis and reducing complications. However, treatment cost of the latter is higher. 2020 Vol. 26 (7): 1125-1129 [Abstract] ( 79 ) HTML (1 KB)  PDF (1232 KB)  ( 368 ) 1130 Correlation Analysis Between Levels of Four Serum Lipid Indexes Serum Homocysteine and Occurrence of Acute Cerebral Infarction in Patients with H-type Hypertension WANG Junjun, WANG benxiao, TANG Yongliang, et al DOI: 10.3969/j.issn.1006-6233.2020.07.018 Objective: To analyze the correlation between levels of four serum lipid indexes, serum homocysteine (Hcy) and occurrence of acute cerebral infarction (ACI) in patients with H-type hypertension. Methods: Eighty patients with H-type hypertension who were admitted to the hospital from August 2018 to July 2019 were enrolled as study objects. According to presence or absence of ACI, they were divided into ACI group (n=30) and non-ACI group (n=50). Another 40 healthy people after physical examination in the hospital during the same period were enrolled as control group. The general data, levels of four serum lipid indexes [triglyceride (TG), cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C)] and serum Hcy were compared among the three groups. The correlation between levels of four serum lipid indexes, serum Hcy and occurrence of ACI in patients with H-type hypertension was analyzed. Results: The systolic blood pressure in ACI group and non-ACI group was higher than that in control group (P<0.05). Serum TC, TG, LDL-C and Hcy levels in ACI group were higher than those in non-ACI group (P<0.05), while serum HDL-C levels were lower than non-ACI group (P<0.05). Serum TC, TG, LDL-C and Hcy levels were positively correlated with the occurrence of ACI (P<0.05), while serum HDL-C level was negatively correlated with it (P<0.05). Serum TC, TG, HDL-C, LDL-C and Hcy were influencing factors of ACI in patients with H-type hypertension (P<0.05). Conclusion: There is correlation between levels of four serum lipid indexes, serum Hcy and occurrence of ACI in patients with H-type hypertension. Clinical monitoring on levels of four serum lipid indexes and serum Hcy is of important value for assessing occurrence of ACI in patients with H-type hypertension. 2020 Vol. 26 (7): 1130-1132 [Abstract] ( 123 ) HTML (1 KB)  PDF (1199 KB)  ( 233 ) 1133 An analysis of the Clinical Value of Plasma miR-92a and miR-93 in the Diagnosis and Short-term Prognosis of Acute ST Segment Elevation Myocardial Infarction XIU Mingwen, BIAN Yuyao DOI: 10.3969/j.issn.1006-6233.2020.07.019 Objective: To investigate the clinical value of plasma miR-92a and miR-93 in the diagnosis and short-term prognosis of acute ST segment elevation myocardial infarction. Methods: 160 patients with acute ST segment elevation myocardial infarction confirmed by emergence department of our hospital were selected into case group, 152 healthy adults were selected into control group. The plasma levels of miR-92a and miR-93 were measured in the two groups. ROC curve was used to evaluate the diagnostic value of plasma miR-92a and miR-93 in patients with acute ST segment elevation myocardial infarction. The incidence of major cardiovascular events (MACE) in patients with acute ST segment elevation myocardial infarction was observed after 3 months follow-up. Logistic regression analysis was used to explore the influencing factors of MACE in patients with acute ST segment elevation myocardial infarction. Results: Compared with the control group, the plasma level of miR-92a was significantly decreased at 4h, 8h, 12h, 24h, and 48h in the case group, while the level of miR-93 increased(P<0.05). At 72h and 1 week after the onset, there were no significant changes in miR-92a and miR-93 levels between the case group and the control group(P>0.05). MiR-92a was positively correlated with HDL-C, and miR-93 was positively correlated with TG, LDL-C, hs-CRP, NT-proBNP; miR-92a was negatively correlated with TG, LDL-C, hs-CRP, NT-proBNP, and miR-93 was negatively correlated with HDL-C(P<0.05). The AUC, sensitivity, specificity, accuracy and cutoff of miR-93 were 0.851, 76.3%, 79.6%, 82.6%, and 2.35, respectively. The AUC, sensitivity, specificity, accuracy and cutoff of miR-92a were 0.846, 75.9%, 72.6%, 81.1%, and 3.59, respectively. The incidence of MACE in the miR-92a high-expression group was higher than that of the miR-92a low-expression group, and the incidence in the miR-93 high-expression group was lower than that of miR-93 low expression group(P<0.05). Logistic regression analysis showed that high-level miR-93 and low-level miR-92a were risk factors for MACE in patients with acute ST-segment elevation myocardial infarction (OR=4.62, 5.02, P<0.05). Conclusion: The level of miR-92a in patients with acute ST segment elevation myocardial infarction is lower, and miR-93 is higher. MiR-92a and miR-93 are expected to be biomarkers for the diagnosis of acute ST segment elevation myocardial infarction. High level miR-93 and low level miR-92a are risk factors for poor prognosis in patients with acute ST segment elevation myocardial infarction. 2020 Vol. 26 (7): 1133-1138 [Abstract] ( 98 ) HTML (1 KB)  PDF (1343 KB)  ( 277 ) 1139 An Analysis of the Effects of Laparoscopic Kimura Method and Warshaw Method on Perioperative Indexes and Postoperative Complications in Patients with Benign Pancreatic Body and Tail Lesions LIU Fengling, ZHU Zhuoli, ZHANG Daizhong, et al DOI: 10.3969/j.issn.1006-6233.2020.07.020 Objective: To observe the treatment effects and safety of laparoscopic Kimura method and Warshaw method on benign pancreatic body and tail lesions. Methods: Retrospective analysis was performed on clinical data of 83 patients who underwent laparoscopic spleen-preserving distal pancreatectomy in our hospital from January 2015 to October 2018. The patients were divided into Kimura group (49 cases) and Warshaw group (34 cases) according to different surgical methods. The perioperative indexes, and platelet changes and immune function changes before and after surgery were recorded in the two groups. The occurrence of complications were recorded in the two groups, and the long-term prognosis was evaluated with follow-up. Results: The operative time in Kimura group was significantly longer than that in Warshaw group (P<0.05), but there were no significant differences in the intraoperative blood loss, postoperative exhaust time, fasting time, hospital stay and somatostatin application time between the two groups (P>0.05). There was no significant difference in platelet level in the two groups at 1 week, 1 month and 3 months after surgery compared with that before surgery (P>0.05), and there was no difference between groups at the same time point (P>0.05). The levels of CD3+, CD4+ and CD4+/CD8+ in Kimura group at 1 week and 1 month after surgery were not significantly different from those before surgery (P>0.05), and the levels of CD3+and CD4+ at 1 week after surgery in Warshaw group were significantly lower than those before surgery (P<0.05), and there were no significant differences at 1 month after surgery compared with those before surgery (P>0.05), but there were no statistically significant differences in the levels of CD3+, CD4+ and CD4+/CD8+ between the two groups at the same time point (P>0.05). The incidence rate of postoperative splenic infarction and the total incidence rate of complications were 0.00% and 10.20% in Kimura group, which were lower than those in Warshaw group with 11.76% and 29.41% (P<0.05). All patients were followed up for 12 to 37 months. No recurrence or reoperation was found. Conclusion: Both laparoscopic Kimura method and Warshaw method are effective methods for spleen-preserving distal pancreatectomy, and have similar effects on platelet and immune function of patients. Although Kimura method takes longer, it can reduce the incidence rate of complications. Warshaw method is more suitable for those with a large tumor range and difficulty in separating from the spleen blood vessels. 2020 Vol. 26 (7): 1139-1143 [Abstract] ( 126 ) HTML (1 KB)  PDF (1244 KB)  ( 387 ) 1144 The Effect of Radiotherapy on DC Cell in Peripheral Blood of Patients with Lung Cancer BAI Lu, LIU Chengyi, ZHANG Jingjing, et al DOI: 10.3969/j.issn.1006-6233.2020.07.021 Objective: To discuss the dynamic changes of DC cell in peripherd blood of patients with lung cancer before and after different radiotherapy dose. Methods: 30 patients with lung cancer were included in the study. To detect DC cell count, live DC cell number, DC number of apoptotic cells and DC cell activity in Peripheral blood of 30 lung cancer radiotherapy patients by MACS(magnetic activated cell sorting) before and after radiotherapy. Results: The number of DC cells increased gradually at 2000cGy, 4000cGy and 6000cGy by radiotherapy, F=8.53, P < 0.001. The number of live DC cells increased at first and then decreased. The highest level at 4000cGy by radiotherapy, and declined at 6000cGy, and 4000cGy by radiotherapy, F=6.88 and P < 0.05 compared with 2000cGy and 6000cGy by radiotherapy. There was no significant change in the number of apoptotic cells in DC, which was the highest at 4000cGy by radiotherapy. The dose of 6000cGy compared with 1 week before radiothrapy, the dose of 2000cGy and 4000cGy, F=3.68 and P=0.015. The changes of the activity of DC cells in the serum of the patients with lung cancer before and after radiotherapy have no significant difference in the activity of DC cells, F=0.839, P=0.476. Conclusion: Radiation therapy can increase the level of DC cells in peripheral blood of lung cancer patients and promote the action of tumor antigen. 2020 Vol. 26 (7): 1144-1147 [Abstract] ( 149 ) HTML (1 KB)  PDF (2277 KB)  ( 378 ) 1148 Effect Difference of Sequential nCPAP and High-flow Nasal Catheter Humidification Oxygen Therapy after Mechanical Ventilation Withdrawal in Neonatal Pneumonia with Respiratory Failure HE Yanzhi, LI Yanjuan DOI: 10.3969/j.issn.1006-6233.2020.07.022 Objective: To investigate the efficacy of sequential nasal continuous positive airway pressure (NCPAP) and humidified high-flow nasal cannula (HHFNC) after weaning from mechanical ventilation on neonatal pneumonia with respiratory failure. Methods: A total of 110 children patients with neonatal pneumonia complicated with respiratory failure admitted to our hospital were divided into control group (55 cases) and observation group (55 cases) by simple randomization grouping according to the random number table method. Control group was given NCPAP treatment after weaning from mechanical ventilation, and observation group was given HHFNC treatment after weaning from mechanical ventilation. The clinical efficacy, blood gas analysis indicators and respiratory rate, oxygenation index, inflammatory factors, immune function indicators and complications were analyzed in the two groups. Results: There was no significant difference in the total effective rate of treatment between the two groups (P>0.05). Before treatment, there were no statistically significant differences in the partial pressure of arterial oxygen (PaO2), partial pressure of arterial blood carbon dioxide (PaCO2), blood oxygen saturation (SaO2), oxygenation index (P/F) and respiratory rate between the two groups (P>0.05). After 24h of treatment, the differences of SaO2 and P/F before and after treatment in observation group were significantly higher than those in control group (P<0.05), and there were no significant differences in the differences of other indicators compared with those in control group (P>0.05). Before treatment, there were no statistically significant differences in the levels of inflammatory factors [interleukin-6 (IL-6), C-reactive protein (CRP), procalcitonin (PCT)] and immune function indexes [immunoglobulins (IgA, IgM, IgG)] between the two groups (P>0.05). After 24h of treatment, the differences of inflammatory factors and immune function indexes before and after treatment in observation group were significantly greater than those in control group (P<0.05). During treatment, the incidence rate of complications in observation group was significantly lower than that in control group (P<0.05). Conclusion: Sequential HHFNC after weaning from mechanical ventilation has similar efficacy with NCPAP in the treatment of neonatal pneumonia with respiratory failure, but sequential HHFNC can effective improve the inflammatory response, enhance the immune function of children patients, and reduce the risk of complications. 2020 Vol. 26 (7): 1148-1151 [Abstract] ( 90 ) HTML (1 KB)  PDF (1222 KB)  ( 298 ) 1152 Analysis of clinical and pathological characteristics in adults patients with ANCA associated vasculitis and renal involvement TANG Hailian, LI Jianjun, BI Jie, et al DOI: 10.3969/j.issn.1006-6233.2020.07.023 Objective: To investigate the clinical and pathological characteristics of different age groups in adults with ANCA-associated vasculitis and renal damage. Methods: 76 adult patients (age ≥ 18 years) were selected and divided into groups according to age. The patients with age ≥ 60 years old were 36 cases in the elderly group, including 12 males and 24 females; the patients with age ≤ 18 years old < 60 years old were 40 cases in the young and middle age group, including 13 males and 27 females. The clinical data and renal pathological results of the two groups were retrospectively analyzed. Results: In adult patients with ANCA associated vasculitis and renal damage,the clinical manifestations of cough, moderate to severe renal insufficiency, anemia, hypertension and gross Hematuria in the observation group were significantly higher than those in the control group (P<0.05). The pathological types of the two groups were mainly segmental type, and the old aged group accounted for 36.1%, the Middle-aged young group accounted for 67.5%; apart from segmental type, crescentic and scleorotic typeswas more common, accounted for 27.8% and 22.2% in the observation group; crescentic type accounted for 20.0% in the control group. The prevalence of crescent formation, glomerular scleorosis, interstitial fibrosis and tubular atrophy were significantly higher in the observation group than those in the control group, and the differences were statistically significant (P<0.01). Conclusions: The clinical manifestations of elderly patients with renal impairment of ANCA-associated vasculitis are more complicated, especially in the aspects of respiratory system and renal function. With various pathological types, both active and chronic renal lesions in the elderly patients are significant. Severe glomerular damage and tubulointerstitial damage are common. 2020 Vol. 26 (7): 1152-1156 [Abstract] ( 109 ) HTML (1 KB)  PDF (1240 KB)  ( 300 ) 1157 Research on the Relevance Between Expressions of ER and PR and Postoperative Recurrence of Uterine Fibroids XU Xu DOI: 10.3969/j.issn.1006-6233.2020.07.024 Objective: To study the relationship between the expressions of estrogen receptor (ER) and progesterone receptor (PR) and postoperative recurrence in patients with uterine fibroids. Methods: 92 cases of patients with uterine fibroids admitted to our hospital were included in the study. 50 patients who underwent initial surgery were included in initial surgery group, and 42 patients who underwent surgery due to recurrence of fibroids were included into recurrent surgery group, and the expressions of ER and PR in fibroids tissues and adjacent tissues were compared between the two groups. According to the quantity of recurrent fibroids, 42 patients with recurrent surgery were divided into mono-recurrence group and multi-recurrence group, and the expressions of ER and PR in fibroids tissues were compared. Logsitic analysis was used to analyze the relationship between expression levels of ER and PR and postoperative recurrence in uterine fibroids tissues. Results: The positive rates of ER and PR in fibroids tissues in initial surgery group and recurrent surgery group were significantly higher than those in adjacent tissues (P<0.05), and the positive rates of ER and PR in recurrent surgery group were significantly higher than those in initial surgery group (P<0.05). The positive cell counts of ER and PR in fibroids tissues in multi-recurrence group were significantly higher than those in mono-recurrence group (P<0.05). Logistic analysis suggested that the high expressions of ER and PR in fibroids tissues were independent risk factors for postoperative recurrence in patients with uterine fibroids. Conclusions: The expression levels of ER and PR in fibroids tissues of patients with uterine fibroids are higher than those in adjacent tissues. The levels of ER and PR in fibroids tissues of recurrent patients are higher than those of patients with initial surgery, the more the recurrent fibroids, the higher the levels of ER and PR in fibroids tissues. The high expressions of ER and PR in tumor tissues are independent risk factors for postoperative recurrence in patients with uterine fibroids. 2020 Vol. 26 (7): 1157-1159 [Abstract] ( 137 ) HTML (1 KB)  PDF (1193 KB)  ( 335 ) 1160 A Comparative Study of Paclitaxel Combined with Tigiao and Oxaliposide Combined with Tigio in Adjuvant Chemotherapy of Gastric Cancer WANG Chunmei, ZHOU Guozhi DOI: 10.3969/j.issn.1006-6233.2020.07.025 Objective: To compare the effect of the combination of paclitaxel and oxaliplatin in the adjuvant chemotherapy of gastric cancer (GC). Methods: 73 patients with advanced GC diagnosed and treated in our hospital from October 2016 to October 2018 were selected as the main subjects. All of them were diagnosed by operation and pathology, and according to the simple random digital table method, divided into the observation group (n=36, chemotherapy of Oxaliposide and Tigiao) and the control group (n =37, chemotherapy of Tigiopaclitaxel and Tigiao).The recent curative effect and the change of the immune function index of the two groups were observed.The overall survival time (OS) of progression-free survival (PFS) was recorded as of October 31,2019, and also the recurrence rate of the two groups and the occurrence of the adverse reactions were recorded. Results: There was no significant difference between the two groups of disease control rate, the postoperative recurrence rate, the PFS and the OS (Pall> 0.05).The levels of Ig A, IgG and IgM after chemotherapy in the two groups were significantly higher than those before chemotherapy(Pall< 0.05).Compared with the control group, the levels of Ig A and IgG in the observation group were significantly higher than those in the control group (Pall< 0.01).The proportion of neutrophils in the observation group was 25.71%, which was significantly lower than that in the control group (48.57%,P < 0.05). Conclusion: The paclitaxel + tigecycline and oxaliplatin + teggio therapies can achieve similar near-term efficacy and survival time. But the oxaliplatin + teggio therapy can improve the immune function of the GC patients and improve the tolerance of the patients, and is worthy of clinical promotion and application. 2020 Vol. 26 (7): 1160-1164 [Abstract] ( 171 ) HTML (1 KB)  PDF (1386 KB)  ( 616 ) 1165 Research on the Effect of Intracoronary Injection of Sodium Nitroprusside plus Recombinant Human Pro-urokinase on the Cardiac Function and Prognosis in Patients Without Reflow During Emergency PCI WANG Yong, GAO Jiangyan, LV Pei, et al DOI: 10.3969/j.issn.1006-6233.2020.07.026 Objective: To explore the clinical efficacy of intracoronary injection of sodium nitroprusside plus recombinant human pro-urokinase in the treatment of acute ST elevation myocardial infarction (STEMI) after emergency percutaneous coronary intervention (PCI) without reflow and its effect on the cardiac function and prognosis in patients. Methods: The subjects, 60 STEMI patients who did not see reflow during emergency PCI in our hospital from March 2017 to March 2019, were divided into observation group (n=30) and control group (n=30) on the basis of random number table. The observation group was treated with intracoronary injection of sodium nitroprusside plus recombinant, and the control group, sodium nitroprusside in the coronary arteries. The two groups were compared in blood flow classification of thrombolysis in myocardial infarction (TIMI) before and after treatment, bleeding complication rate during and within 24h hrs after the operation, cardiac function parameters [left ventricular end diastolic diameter (LVEDD), left ventricular ejection fraction (LVEF) ] on the 7th day and at the end of the 1st month after the operation as well as the occurrence of major adverse cardiac events (MACE) within 1 month after operation. Results: After treatment, 28 patients from the observation group (93.33%) and 20 patients from the control groups (66.67%) were grade 3 in TIMI blood flow classification (93.33%). The observation group saw much better TIMI blood flow classification than the control group did (P<0.05). At the end of the 1st month after the operation, both groups witnessed remarkably lower LVEDD but higher LVEF as compared with those on the 7th day after the operation (P<0.05), and at the end of the 1st month after the operation, the observation group had obviously lower LVEDD but higher LVEF than the control group did over the same period (P<0.05). The bleeding rate of the observation group during and within 24h after the operation was 16.67% (5/30), seeing no big difference from 10.00% (3/30) of the control group (P>0.05). The incidence of MACE of the observation group within 1 month after the operation was 6.67% (2/30) which was lower than 20.00% (6/30) of the control group, but the difference was not statistically significant (P>0.05). Conclusion: In the treatment of acute STEMI after emergency PCI without reflow, the application of intracoronary injection of sodium nitroprusside plus recombinant can effectively restore coronary blood flow and myocardial reperfusion in patients and improve their cardiac function and prognosis, proved to be safe and reliable. 2020 Vol. 26 (7): 1165-1169 [Abstract] ( 108 ) HTML (1 KB)  PDF (1210 KB)  ( 291 ) 1170 Clinical Comparison of 3% Hypertonic Saline and 20% Mannitol in the Treatment of Severe Craniocerebral Injury with Intracranial Hypertension SONG Mengjiao, ZHANG Lei, ZHOU meihui, et al DOI: 10.3969/j.issn.1006-6233.2020.07.027 Objective: To explore and compare the clinical efficacy of 3% hypertonic saline (HTS) and 20% mannitol (MT) in the treatment of severe craniocerebral injury with intracranial hypertension. Methods: A total of 100 patients with severe craniocerebral injury complicated with intracranial hypertension diagnosed and treated in our hospital from January 2018 to February 2019 were selected as the study objects and divided into treatment group and control group according to the random number table method, with 50 cases in each group. 3% HTS was administered to the treatment group and 20% MT was administered to the control group. The magnitude and duration of ICP decline before and after treatment were compared between the two groups, and the changes of MAP, CVP, CPP, serum sodium and plasma osmotic pressure at different times and the complications after treatment were recorded. Results: Repeated measures of variance analysis showed that the interaction effects of ICP, MAP, CPP, serum sodium and plasma osmotic pressure in the two groups were statistically significant at the time point, between the groups, and between the time point and the group (P<0.05), and the interaction effects of CVP were statistically significant at the time point and the group (P<0.05).After 1, 3 and 6 h of administration, ICP in the treatment group was lower than that in the control group, and MAP, CVP, CPP, serum sodium and plasma osmotic pressure were higher than those in the control group, with statistically significant differences (P<0.05).The other two groups were accompanied by cerebral infarction, delayed intracranial hematoma, diffuse brain swelling and other complications, the overall incidence of the treatment group was 6.00%, the control group was 14.00%, and the difference between the two groups was not statistically significant (χ2=1.778, P>0.05). Conclusion: 3% HTS can effectively reduce ICP in patients with severe craniocerebral injury, which is conducive to improving cerebral perfusion in patients, and has little impact on body circulation, which is worthy of clinical promotion. 2020 Vol. 26 (7): 1170-1174 [Abstract] ( 129 ) HTML (1 KB)  PDF (1213 KB)  ( 288 ) 1175 Research on the Application of DWI-MRI in the Evaluation of Chemoradiotherapy Efficacy in Cervical Cancer QIU Wenwei, PENG Yubin, et al DOI: 10.3969/j.issn.1006-6233.2020.07.028 Objective: To analyze the application value of diffusion-weighted magnetic resonance imaging (DWI-MRI) in the evaluation of chemoradiotherapy efficacy in cervical cancer. Methods: The clinical data of 164 patients with cervical cancer who underwent chemoradiotherapy were retrospectively analyzed. The changes of apparent diffusion coefficient (ADC) were recorded in 164 patients before chemoradiotherapy and at 2, 4 and 8 w of chemoradiotherapy, and the patients were divided into complete remission (CR) group, partial remission (PR) group, stability of disease (SD) group and progression of disease (PD) group according to the efficacy at the end of chemoradiotherapy. The ADC values of each group before and after chemoradiotherapy were compared. Results: The ADC values of 164 patients at each time point after chemoradiotherapy were higher than those before chemoradiotherapy (P<0.05), and the value showed 8 w of chemoradiotherapy > 4 w of chemoradiotherapy > 2 w of chemoradiotherapy (P<0.05). Among 164 patients, 44 cases with CR were enrolled in CR group, and 96 cases with PR were included in PR group, and 24 cases with SD were included in SD group and 0 case in PD group. Before chemoradiotherapy, there was no significant difference in ADC value among the three groups (P>0.05). After chemoradiotherapy, the ADC values in the three groups were higher than those before chemoradiotherapy (P<0.05), and the values showed CR group > PR group > SD group (P<0.05). Conclusion: DWI-MRI has a high application value in the evaluation of chemoradiotherapy efficacy in cervical cancer, and it can as early as possible diagnose the efficacy of chemoradiotherapy, and it is favorable for clinical treatment. 2020 Vol. 26 (7): 1175-1177 [Abstract] ( 130 ) HTML (1 KB)  PDF (1554 KB)  ( 318 ) 1178 A Clinical Study on Changes of Serum NT-proBNP BMP-7 and TGF-β1 Levels in Premature Infants with Respiratory Distress Syndrome QI Changli, et al DOI: 10.3969/j.issn.1006-6233.2020.07.029 Objective: To explore the clinical study on changes of serum Amino-terminal pro-brain natriuretic peptide (NT-proBNP), Bone morphogenetic protein -7 (BMP-7) and Transforming growth factor-β1 (TGF-β1) levels in premature infants with respiratory distress syndrome (RDS). Methods: A total of 106 premature infants with RDS diagnosed in the hospital from April 2016 to April 2018 were selected, and another 53 premature infants with non-RDS of the same age group at the same time period were collected as control group. The expression levels of NT-proBNP, BMP-7 and TGF-β1 in serum samples of premature infants were detected by ELISA in the two groups. The correlation between NT-proBNP, BMP-7 and TGF-β1 and disease severity was analyzed by Pearson correlation analysis. The ROC curve was used to analyze its diagnostic value for RDS. Results: Compared with control group, the expression levels of BMP-7, NT-proBNP and TGF-β1 in serum of RDS patients were significantly increased (P<0.05). Serum NT-proBNP, BMP-7 and TGF-β1 in premature infants with RDS were positively correlated with disease severity (r=0.402, P=0.000; r=0.311, P=0.018; r=0.313, P=0.015). The cut-off value, sensitivity, specificity and AUC of NT-proBNP in the diagnosis of premature infants with RDS were 3655.92 ng/L, 88.7%, 3.8% and 0.985. The cut-off value, sensitivity, specificity and AUC of BMP-7 diagnosis were 44.85 ng/L, 63.2%, 11.3% and 0.829. The cut-off value, sensitivity, specificity and AUC of TGF-β1 diagnosis were 36.73 g/L, 81.1%, 17.0% and 0.829. The sensitivity, specificity and AUC of BMP-7 combined with TGF-β1 were 89.6%, 15.1% and 0.934. Conclusion: The levels of serum NT-proBNP, BMP-7 and TGF-β1 in RDS patients are significantly increased, and their expression levels are positively correlated with disease severity, and they are expected to be diagnostic and prognostic markers. 2020 Vol. 26 (7): 1178-1181 [Abstract] ( 106 ) HTML (1 KB)  PDF (1257 KB)  ( 382 ) 1182 Observation on Effect of Butylphthalide Soft Capsule on Early Senile Parkinson's Disease JIANG Ying, LIANG Jianfen, ZHANG Xingbo, et al DOI: 10.3969/j.issn.1006-6233.2020.07.030 Objective: To investigate the clinical efficacy of butylphthalide soft capsule in the treatment of early senile Parkinson's disease (PD) and analyze its possible mechanism through the change of serological indexes. Methods: 82 PD patients from January 2017 to December 2018 were selected and divided into control group and observation group according to the random number table, 41 cases each. Two groups of patients were given dopaminergic drugs, the observation group on this basis combined with soft capsules of butylphthalide oral, the treatment cycle was 12 weeks. Before and after treatment, Hoehn & Yahr classification (H-Y classification) and mds-updrs (Part 1 and Part 3) were used to evaluate the clinical efficacy. Meanwhile, the activities of superoxide dismutase (SOD), malondialdehyde (MDA), interleukin-6 (IL-6) and neuron specific enolase (NSE) were measured. Results: The MDS-UPDRS-1, MDS-UPDRS-3, and HY scores of Parkinson's disease patients in the observation group after treatment were lower than those in the control group. After treatment in the control group, their SOD, MDA, Both IL-6 and NSE improved in different degrees. After treatment, their SOD activity in the observation group increased significantly compared with that before treatment, and their MDA, IL-6, and NSE significantly decreased compared with before treatment. The difference was statistically significant (P<0.05). Conclusion: The Butylphthalide soft capsules combined with Dopaminergic drugs can reduce the H-Y grade, MDS-UPDRS-1, MDS-UPDRS-3 scores of early PD patients, improve their SOD, MDA, IL-6, NSE levels, and improve the prognosis of patients with PD. 2020 Vol. 26 (7): 1182-1185 [Abstract] ( 160 ) HTML (1 KB)  PDF (1222 KB)  ( 312 ) 1186 Analysis on the Clinical Effect and Survival Factors of Bortezomib Based Chemotherapy in Patients with Multiple Myeloma HUANG Yali, YANG Liping, XIE Zhijun, et al DOI: 10.3969/j.issn.1006-6233.2020.07.031 Objective: To investigate the clinical efficacy of bortezomib-based chemotherapy regimen in the treatment of multiple myeloma (MM) and to analyze the related factors affecting the prognosis of patients. Methods: The clinical data of 80 patients with MM who received bortezomib-based chemotherapy regimen were retrospectively analyzed. The efficacy was evaluated, and Kaplan-Meier method was used for survival analysis, and the 3-year overall survival (OS) rate and progression-free survival (PFS) rate were estimated. Log-rank test and Cox proportional hazard regression model were used for univariate analysis and multivariate analysis on factors that might affect prognosis. Results: Of the 80 patients, the total effective rate was 82.5% and the CR rate was 43.8%. The median follow-up time was 28 (2~89) months, and the 3-year OS of the whole group was 76.4%, and the PFS rate was 57.8%. Univariate analysis showed that prognosis of MM patients was related to age, ISS staging, white blood cell count, homocysteine (Hcy) level, platelet count (PLT), lactate dehydrogenase level, Kappa light chain level, FISH results, efficacy and presence or absence of HSCT treatment (P<0.05), and was not related to gender, DS staging, hemoglobin level, serum creatinine level, urea nitrogen level, bone marrow plasma cell number, MM type, albumin, chromosome condition, bone damage and kidney damage (P>0.05). Cox multivariate analysis showed that PLT≤100×109L-1, Hcy≥22μmol/L and Kappa light chain level≥19.4mg/L were independent risk factors for the prognosis (OS and PFS) of MM patients (P<0.05), and efficacy ≥ VGPR was a beneficial factor for prognosis (OS) (P<0.05). Conclusion: Bortezomib-based chemotherapy regimen has significant efficacy in the treatment of MM. PLT, Hcy and Kappa light chain level are independent risk factors for survival of patients, and efficacy ≥ VGPR is a beneficial factor for survival. 2020 Vol. 26 (7): 1186-1191 [Abstract] ( 105 ) HTML (1 KB)  PDF (1297 KB)  ( 406 ) 1192 Results of CT-guided Needle Biopsy for Solitary Pulmonary Nodules of Different Sizes GAO Wanchun, LI Xiaojun, TAN Binbin, et al DOI: 10.3969/j.issn.1006-6233.2020.07.032 Objective: To analyze the diagnostic results of CT-guided needle biopsy for solitary pulmonary nodules of different sizes. Methods: A total of 108 patients with solitary pulmonary nodules admitted to our hospital from December 2013 to December 2018 were selected and grouped according to the size of lesions. The patients with size>2 cm were set as group A (n=58), and those with size ≤2 cm were set as group B (n=50). CT-guided needle biopsy was performed in the two groups. The needle biopsy results, surgical pathological results and complications were compared between the two groups. Results: The success rate of one-time puncture in group A was significantly higher than that in group B (93.10% vs 80.00%) (χ2=4.086, P<0.05). The accuracy rate of diagnosis in group A was significantly higher than that in group B (89.66% vs 74.00%), the puncture frequency in group A was significantly lower than that in group B [(1.07±0.25) vs (1.20±0.38)]. (χ2=4.539, t=2.126, P<0.05). The incidence rate of complications in group A was significantly lower than that in group B (13.79% vs 30.00%) (χ2=4.208, P<0.05). Conclusion: CT-guided needle biopsy is a simple and reliable technique, and it can accurately identify the benign and malignant solitary pulmonary nodules. However, it has low success rate of puncture, low accuracy rate of pathological diagnosis and many complications on solitary pulmonary nodules with lesion size≤ 2cm, and there is still a certain risk. 2020 Vol. 26 (7): 1192-1194 [Abstract] ( 121 ) HTML (1 KB)  PDF (1206 KB)  ( 597 ) 1195 Analysis of Incidence and Risk Factors of Urethral Stricture After Transurethral Prostate Plasmatomy FENG Wei, ZHU Xiaocong, HU Yafang DOI: 10.3969/j.issn.1006-6233.2020.07.033 Objective: To analyze the incidence and risk factors of urethral stricture after transurethral prostate plasmatomy (PKRP). Methods: 541 patients with PKRP admitted to our hospital from January 2015 to March 2019 were selected as the research objects, followed up for 6 months to analyze the occurrence of postoperative urethral stricture. According to whether or not urethral stricture was complicated, they were divided into two groups: urethral stricture group and urethral normal group. Compared the perioperative information of the two groups, the factors with statistical significance were analyzed by logistic regression analysis. Pearson correlation was used to analyze the relationship between the independent risk factors and the maximum flow rate in patients with PKRP posterior urethral stricture. Results: Among 541 patients who received PKRP, 532 cases were followed up, with a follow-up rate of 98.34%; 31 cases of urethral stricture, with a incidence of 5.83%; by multiple factor Logistic regression analysis, preoperative urethral infection, intraoperative urethral dilatation and plastic surgery, continuous traction time, operation time and postoperative catheter retention time were independent risk factors of urethral stricture after PKRP (P<0.05); According to Pearson correlation analysis, the duration of traction, operation time and indwelling catheter time were negatively correlated with the maximum urinary flow rate (r-3.642, - 2.985, - 5.478, P 0.016, 0.025, 0.000, respectively); According to ROC curve analysis, the AUC of urethral stricture in PKRP patients with preoperative urethral infection or intraoperative urethral dilatation was 0.915, which was significantly higher than that of single index (P<0.05). Conclusion: The urethral stricture after PKRP is not uncommon. It is related to preoperative urethral infection, intraoperative urethral dilatation, continuous traction time, operation time and postoperative indwelling catheter time. The analysis of risk factors of urethral stricture after PKRP is helpful to predict the occurrence of urethral stricture, which is worthy of clinical attention. 2020 Vol. 26 (7): 1195-1199 [Abstract] ( 87 ) HTML (1 KB)  PDF (1433 KB)  ( 273 ) 1200 Comparison on Effects of Remifentanil or Fentanyl Combined with Propofol General Anesthesia on Cardiac Function and Hemodynamic Parameters in Elderly Patients Undergoing Surgery CHEN Yuanli, XIE Jian, CHEN Fei DOI: 10.3969/j.issn.1006-6233.2020.07.034 Objective: To investigate the effects of remifentanil or fentanyl combined with propofol general anesthesia on cardiac function and hemodynamic parameters in elderly patients. Methods: A total of 220 elderly patients admitted to our hospital from December 2017 to June 2019 were randomly divided into observation group (110 cases) and control group (110 cases). The changes of heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial blood pressure (map), left ventricular ejection fraction (LVEF), cardiac stroke volume (SV), cardiac output (CO), cardiac index (CI) were compared before anesthesia induction (t0), before tracheal intubation (T1), immediately after tracheal intubation (T2), at the time of skin incision (T3) and 10 minutes after operation (T4) The operation related indexes and complications of the two groups were recorded. Results: There was no significant difference in HR, SBP, DBP, map, LVEF%, SV, CO and CI between the two groups in t0 and T4 period (P > 0.05). The time of extubation, time of opening eyes, time of spontaneous respiration, time of recovery of orientation force and incidence of adverse reactions in the observation group were significantly lower than those in the control group (P < 0.05). Conclusion: Remifentanil combined with propofol for general anesthesia can stabilize the patient's heart function and hemodynamics, improve the awake time and safety of patients, and it is worthy of popularization. 2020 Vol. 26 (7): 1200-1204 [Abstract] ( 128 ) HTML (1 KB)  PDF (1241 KB)  ( 311 ) 1205 Clinical Efficacy of Closed Vacuum Sealing Drainage (VSD) Combined with Masquelet Technique for Traumatic Sacral Osteomyelitis DU Yuanliang, XIAO Tianjie, GENG Yanxia DOI: 10.3969/j.issn.1006-6233.2020.07.035 Objective: To investigate the clinical efficacy of focal debridement combined with closed vacuum drainage and Masquelet in the treatment of traumatic osteomyelitis of the tibia. Methods: 59 patients with traumatic tibial osteomyelitis who were hospitalized in our hospital from January 2014 to July 2017 to receive focus clearance and closed negative pressure drainage to control bone infection were selected. 31 patients in the observation group were treated with masquelet technology to repair bone defects, and 28 patients in the control group were treated with direct autogenous bone grafting. The complications, healing time and self-care ability (SF-36) of the two groups were compared. Results: The observation group had lower bone resorption complications and fracture healing time than the control group (P<0.05). The self-care ability score of the observation group was higher than that of the control group at 3 months after operation (P<0.05). Conclusion: Focal debridement combined with closed negative pressure drainage and Masquelet technique for the treatment of traumatic tibiofibular osteomyelitis can shorten the time of bone healing, reduce the complications of osteolysis, and greatly improve the patient's ability to live. 2020 Vol. 26 (7): 1205-1208 [Abstract] ( 102 ) HTML (1 KB)  PDF (1217 KB)  ( 290 ) 1209 Comparative Experimental Study on Three Different Materials for Repairing Oral Mucosa Defects in Rats HAN Yongjie, LIU Shijie, DENG Lu DOI: 10.3969/j.issn.1006-6233.2020.07.036 Objective: To investigate the effects of three kinds of materials, namely, homologous (human skin) acellular matrix, heterogeneous (cowhide) acellular matrix, and nanocellulose protein in the repair of oral mucosa defects in rats. Methods: 80 3-month-old male Wister clean rats were randomly divided into three groups: group A (nanocellulose silk fibroin group, n=20), group B (cowhide acellular matrix group, n=20), group C (human skin acellular matrix group, n=20), and group D (blank control group, n=20). Full-thickness buccal mucosa with a diameter of 10 mm was excised from rats in each group. Wounds of rats in groups A, B, and C were respectively covered with silk fibroin, heterogeneous (cowhide) acellular matrix, and homologous (human skin) acellular matrix which had the same diameter and were treated with radicidation. The wounds of rats in group D were covered with vaseline gauze. The wound areas were compared among groups at 1 week, 2 weeks, 3 weeks and 4 weeks after surgery. The numbers of fibroblasts, neovascular endothelial cells and inflammatory cells, and growth of endothelial cells in rats at different time points were analyzed through HE staining and immunohistochemistry. Results: ①There was no wound infection in each group. Transparent membranes could be observed in the grafted area of group A, B, and C at 1 week after surgery. The wound margin of group D was slightly red and swollen. The wound diameter of group D was significantly larger than that of group A, B, and C at 1 week, 2 weeks, and 3 weeks after surgery (P<0.05), without significant difference in wound size among group A, B, and C at each time point (P>0.05). ② HE staining showed that the numbers of fibroblasts and inflammatory cells in group A, B, and C at different time points were significantly smaller than those in group D (P<0.05), without significant difference among group A, B, and C at each time point (P>0.05). ③Immunohistochemistry showed that the expression of CK-positive cells in group A, B, and C was significantly higher than that in group D at 1 week, 2 weeks, 3 weeks and 4 weeks after surgery (P<0.05). The number of CD34-positive cells was significantly larger than that in group D at 1 week, 2 weeks, and 3 weeks after surgery (P<0.05), without significant difference among group A, B, and C at each time point (P>0.05). Conclusion: The effects of nanocellulose protein, autologous and allogeneic cell matrices are similar in repair of oral mucosa damage in rats. 2020 Vol. 26 (7): 1209-1213 [Abstract] ( 120 ) HTML (1 KB)  PDF (3218 KB)  ( 579 ) 1217 Study on the Antibacterial Activity and Reverse Drug Resistance of Compound Honggen Grass Tablet and Its Components against Multidrug Resistant Bacteria HU Junhui, WANG Ying, et al DOI: 10.3969/j.issn.1006-6233.2020.07.038 Objective: To provide the basis for the antibacterial treatment of traditional Chinese medicine, the in vitro antibacterial activity and reverse drug resistance of 5 kinds of Chinese herbal medicines in compound honggencao tablet and its formulation were studied. Methods: The mic value of the drug-resistant bacteria, Acinetobacter baumannii and Klebsiella pneumoniae, isolated from the sputum of the inpatients, was determined by the method of broth dilution, and the index of combined use ∑ FIC was calculated to judge whether the drug resistance was reversed or not. Results: In vitro antibacterial activity test showed that compound Honggen tablet had no antibacterial effect on Acinetobacter baumannii and had antibacterial effect on Klebsiella pneumoniae. The reverse drug resistance test showed that the compound honggencao tablet had synergistic effect with imipenem, cefoperazone sulbactam and gentamycin respectively. The Chinese herbal medicines with imipenem, cefoperazone sulbactam, gentamycin and cefepime respectively had different synergistic effect on Klebsiella pneumoniae. Conclusion: Compound honggencao tablet and its compound 5 are Chinese herbal medicines, which have certain effect on antibacterial activity and reverse drug resistance of multi drug resistant Klebsiella pneumoniae. 2020 Vol. 26 (7): 1217-1219 [Abstract] ( 99 ) HTML (1 KB)  PDF (1195 KB)  ( 343 ) 1220 A Study of the Curative Effect of Entecavir and Tongluo Ruanjian Capsule for Patients with Posthepatitic Cirrhosis and Their Effect on Liver Function and Liver Fibrosis SHEN Lixian, WANG Xiaoling, LI Huiwen DOI: 10.3969/j.issn.1006-6233.2020.07.039 Objective: To explore the clinical effect of entecavir and Tongluo Ruanjian capsule in the treatment of liver fibrosis after hepatitis B, and to observe the influence of entecavir and Tongluo Ruanjian Capsule on serum indexes, liver function and liver fibrosis. Methods: From January 2015 to January 2017, 120 patients with liver fibrosis after hepatitis B were collected as the study object, and divided into control group and observation group with simple random number table method, 60 cases in each group. Both groups were given basic treatment, including vitamin, diammonium glycyrrhizinate enteric coated capsules, etc. On this basis, the patients in the control group were additionally administered with entecavir tablets; the patients in the observation group were additionally administered with Tongluo Ruanjian capsule. Both groups were treated for 6 months and 2 courses. The changes of serum matrix metalloproteinase-13 (MMP-13), tumor necrosis factor α (TNF - α), transforming growth factor β 1 (TGF - β 1), international standard ratio (INR) and end-stage liver disease model score (MELD) were observed and evaluated before and after treatment. The changes of liver function indexes and liver fibrosis indexes were compared before and after treatment. The clinical efficacy and adverse reactions of the two groups were observed and recorded, and the safety of the two groups was evaluated. Results: After two courses of treatment, the total effective rate of clinical treatment in the observation group was 95.00% (57 / 60), significantly higher than 81.67% (49 / 60) in the control group, and the difference was statistically significant (P < 0.01). The serum inflammatory indexes (MMP-13, TNF - α, TGF - β 1), liver function indexes (ALT, GGT, TB), INR and MELD scores in the two groups were significantly lower than those before treatment, and the improvement degree of each index in the observation group was more significant than that in the control group, with statistical significance (P < 0.01). The index of liver fibrosis in the observation group was significantly lower than that in the control group (P < 0.01). During the treatment, no serious adverse reactions were found in the two groups. Conclusion: Entecavir tablet combined with Tongluo Ruanjian capsule has obvious curative effect for patients with liver fibrosis after hepatitis B, which can significantly improve the liver function and liver fibrosis index, and it is safe. 2020 Vol. 26 (7): 1220-1224 [Abstract] ( 150 ) HTML (1 KB)  PDF (1242 KB)  ( 524 ) 1225 An Experimental Research of the Effect of Self-drafting Woman's Blood Soup on Dysfunctional Uterine Bleeding on Rat Model LI Zongguo, REN Chunyan, SU Xidong, et al DOI: 10.3969/j.issn.1006-6233.2020.07.040 Objective: To study the effect of self-drafting woman's blood soup on dysfunctional uterine bleeding on rat model. Methods: A rat model of dysfunctional uterine bleeding was established. The rats were randomly divided into a model group, a positive control group, and low-dose and high-dose groups, with 10 rats in each group. Another ten pregnant rats were used as a blank group. On the first day after the successful modeling (ie, the 8th day of pregnancy), the low-dose and high-dose groups were respectively given 15--30 g/kg of the self-drafting woman's blood soup. The positive control group was intragastrically given self-drafting woman's blood soup for 0.07 g / kg. The blank group and the model group were intragastrically administered with the same dose of self-drafting woman's blood soup once a day for 7 days. The bleeding time and coagulation function, serum hormone levels and uterus and ovarian index of each group were measured. Results: Compared with the model group, the bleeding time, PT and TT of the positive control group and the low-dose and high-dose groups were significantly shortened, and the FIB was significantly increased (P<0.05). The bleeding time, PT and TT of the low- and high-dose groups were lower than those of the positive control group, and FIB was significantly higher than the positive control group, but there was no significant difference (P>0.05). Compared with the model group, the levels of serum E2, P, and FSH in the positive control group and the low-dose and high-dose groups were significantly decreased, and LH was significantly increased (P<0.05). The low and high dose groups and the positive control group can significantly increase the uterus and ovarian index of rats (P<0.01). Conclusion: The self-drafting woman's blood soup can improve the dysfunctional uterine bleeding of the rat model. 2020 Vol. 26 (7): 1225-1228 [Abstract] ( 111 ) HTML (1 KB)  PDF (1201 KB)  ( 260 ) 1229 Relationship between MiR-221/222 Gene Polymorphism and Heart Failure in Men FAN Chunhong, CHEN Xiaoning DOI: 10.3969/j.issn.1006-6233.2020.07.041 Objective: To investigate the regulation of miR-221/222 on the expression and the relation with heart failure. Methods: The peripheral blood samples from male heart failure patients and healthy volunteers were collected and separated for blood cells and plasma, respectively. The genome DNA was extracted, amplified and sequenced for analyzing the distribution of SNP genotypes in the heart failure patients and healthy controls. The plasma miRNA was extracted and the expression of miR-221/222 was detected with a TaqMan assay. The plasma levels of miR-221/222 were analyzed according to the SNP genotypes in heart failure. Results: The distribution of SNP genotypes of rs2858059、rs2858060、rs2858061 and rs34678647 were all comparable between heart failure patients and healthy controls. The genotype of rs113054794 was only C/- in all of the study subjects. The rs182069485 and rs72808106 in the 3'UTR of p27 and DDIT4 encoding genes showed no polymorphism in all of the subjects. The plasma levels of miR-221 and miR-222 were increased in heart failure patients. More importantly, we observed that the T/- genotype of rs34678647 in miR-222 was related with the increase of plasma miR-222 in heart failure patients. Conclusion: Plasma miR-221/222 levels are increased in male heart failure patients. The genotype of rs34678647 is related with the plasma miR-222 levels in heart failure patients. 2020 Vol. 26 (7): 1229-1232 [Abstract] ( 117 ) HTML (1 KB)  PDF (1311 KB)  ( 254 )

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