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2020 Vol. 26, No. 4 Published: 2020-04-30 529 Diagnostic Value of IL-8 Polymorphism and its Expression Level in the Susceptibility of Nasopharyngeal Carcinoma PAN Guogang, YANG Bixiu, WANG Chunfang, et al DOI: 10.3969/j.issn.1006-6233.2020.04.001 Objective: To analyze the interleukin-8 (IL-8) genotype polymorphism and its expression level in NPC patients, and to explore its diagnostic value in NPC susceptibility. Methods: A total of 168 NPC patients in our hospital from January 2017 to September 2019 were collected as the experimental group. 232 healthy patients in the same period were selected as the control group (NC group). The polymorphism of IL-8 gene - 251, + 781, + 1633 and + 2767 were analyzed by PCR-RFLP, and the serum IL-8 in NPC patients was detected by ELISA The relationship between IL-8 gene polymorphism and NPC susceptibility was evaluated by logistic regression analysis. The interaction of different exposure factors in NPC susceptibility was analyzed. The diagnostic value of IL-8 level in NPC was evaluated by ROC. Results: Compared with the NC group, the risk of NPC in the IL-8-251 gene locus TT genotype (OR=1.98, 95%CI: 1.00 ~ 3.82) and AA genotype (OR=1.76, 95%CI: 1.02 ~ 3.08) was significantly increased, the risk of NPC in the TA genotype (OR=1.35, 95%CI: 1.01~2.61) was significantly reduced, and the expression level of IL-8 in NPC patients was significantly increased (P<0.05); There was a negative interaction between IL-8-251 SNPs and smoking and EBV infection factors in the occurrence of NPC. The area under the curve (AUC) of IL-8 susceptibility to NPC was 0.852, with 86% sensitivity and 78% specificity. Conclusion: The genotype polymorphism at the -251 locus of IL-8 gene and increased expression of serum IL-8 are related to NPC susceptibility, and interact with smoking and EBV infection exposure. IL-8 level is of diagnostic value for NPC susceptibility. 2020 Vol. 26 (4): 529-533 [Abstract] ( 207 ) HTML (1 KB)  PDF (1307 KB)  ( 351 ) 533 Study of Aspirin Low Molecular Weight Heparin Sodium and Rivashaban in the Prevention of Deep Venous Thrombosis of Lower Extremity after Knee Arthroplasty MO Huagui, HUANG Yuanqiao, LUO Yuqiang, et al DOI: 10.3969/j.issn.1006-6233.2020.04.002 Objective: To investigate the preventive effect of aspirin, low molecular weight heparin sodium and rivashaban on deep venous thrombosis of lower extremity after knee arthroplasty. Methods: 200 patients with total knee replacement in our hospital from July 2017 to April 2019 were selected as the subject of the study.The patients with the group A were given an oral rivasahan treatment, and the povtients in group B was given an oral aspirin, and the group C was given low molecular heparin sodium injection treatment. The prothrombin time (PT), activated partial thromboplastin time (APTT), D-dimer) and platelet value (Plt) were observed and compared among the three groups, and the incidence of deep venous thrombosis of lower extremity was recorded. Results: After anticoagulation, the number of effective treatment cases in group A (66 cases) was significantly higher than that in group B (44 cases, 68.75%) and group C (47 cases, 71.21%). The total effective treatment rate was 94.29%, with a significant difference (P<0.05); There was no significant difference in Pt, APTT and PLT indexes between group A and group B (P>0.05), and there was significant difference in three indexes between group A and group C (P<0.05); One day, one week and 15 days after the treatment, the D-dimer value of group A was significantly lower than that of group B (P<0.05); one week and 15 days after the treatment, the D-dimer value of group A was significantly lower than that of group C (P<0.05); two patients in group A had DVT, the incidence was 5.88%; six patients in group B, the incidence was 17.65%; ten patients in group C, the incidence was 29.4%. There was no significant difference between the two groups (χ2 = 2.267, P = 0.132) and the two groups (DVT χ2=1.308, P=0.253,P=0.253, P<0.05). Conclusion: Compared with low molecular weight heparin, aspirin has a higher therapeutic safety, but a lower therapeutic effect; rivaroxaban has a higher anticoagulant effect, but a higher incidence of occult blood loss and incision complications. Therefore, the anticoagulation needs of patients can be reasonably selected according to the actual situation. 2020 Vol. 26 (4): 533-537 [Abstract] ( 150 ) HTML (1 KB)  PDF (1205 KB)  ( 348 ) 537 Efficacy and Side Effects of Bortezomib Combined with Cyclophosphamide and Dexamethasone in the Treatment of Multiple Myeloma LU Jun, ZHU Xijun DOI: 10.3969/j.issn.1006-6233.2020.04.003 Objective: To investigate the clinical efficacy and side effects of bortezomib combined with cyclophosphamide and dexamethasone in the treatment of multiple myeloma (MM). Methods: 70 cases of patients with multiple myeloma from October 2016 to October 2019 were divided into two groups according to the treatment, Bortezomib plus cyclophosphamide and dexamethasone group (cyclophosphamide group, n = 35) and bortezomib plus thalidomide and dexamethasone group (thalidomide group, n = 35). The clinical efficacy and toxicity of the two groups were statistically analyzed. Results: The deep response rate of cyclophosphamide group was 82.9% (29 / 35) significantly higher than that of thalidomide group 51.4% (18 / 35) (P<0.05). The incidence of side effects was 54.3% (19 / 35) in the cyclophosphamide group and 60.0%(21/35) in the thalidomide group. The difference between the two groups was not significant (P>0.05). Conclusion: The therapeutic effect of bortezomib combined with cyclophosphamide and dexamethasone is better than bortezomib combined with thalidomide and dexamethasone, and it will not increase the side effects of patients. 2020 Vol. 26 (4): 537-540 [Abstract] ( 192 ) HTML (1 KB)  PDF (1176 KB)  ( 459 ) 541 The Distribution and Risk Factors of HPV-16 and HPV-58 Subtypes in Cervical Lesions with Persistent Infection LIU Qunxiang, WANG Li, LI Jiangli DOI: 10.3969/j.issn.1006-6233.2020.04.004 Objective:The distribution of HPV-16 and HPV-58 subtypes and the risk factors leading to cervical lesions were analyzed.Methods:From January 2017 to March 2019, 100 patients with cervical lesions were selected as observation group and 90 patients with normal physical examination as control group. The distribution of HPV-16 and HPV-58 subtypes of human papillomavirus (Human papillomavirus,HPV) subtypes in patients with persistent infection in the observation group was analyzed. According to the general data of the two groups, univariate and binary Logistic regression analysis was used to analyze the influencing factors and independent risk factors of cervical lesions in the observation group.Results:The highest proportion of cervical cancer patients was HPV-16 (73.68%), HPV-58 (78.95%), cervical intraepithelial neoplasia II-III (60.00%), HPV-58 (66.67%), cervical intraepithelial neoplasia I (36.84%), HPV-58 (31.58%), cervicitis (35.72%), HPV-58 (46.43%); the distribution of HPV-16 and HPV-58 in different types of cervical lesions was statistically significant (P<0.05). Single factor and binary logistic regression analysis showed that age of first sexual behavior, number of sexual partners, vaginitis and family history of cervical cancer were the influencing factors and independent risk factors of cervical lesions.Conclusion: The proportion of HPV-16 and HPV-58 in the patients with cervical intraepithelial neoplasia (HPV-16) and HPV-58 (HPV-58) in the patients with cervical intraepithelial neoplasia (HPV-16), followed by cervical intraepithelial neoplasia (II-III), I-phase, and at least cervicitis (Cervicitis). The age of initial sexual behavior, the number of sexual partners, the inflammation of the vagina, the family history of the cervical cancer are the independent risk factors that lead to the cervical lesion. 2020 Vol. 26 (4): 541-545 [Abstract] ( 147 ) HTML (1 KB)  PDF (1201 KB)  ( 509 ) 545 Relationship between Serum Lipid Levels RDW and ET-1 andIn-stent Restenosis after PCI in Patients with CHD LIU Hengyou, LU Jinguo, LIU Xiaohe, et al DOI: 10.3969/j.issn.1006-6233.2020.04.005 Objective: To study the relationship between control blood lipid level, erythrocyte distribution width (RDW) and endothelin-1 (ET-1) and in-stent restenosis (ISR) after percutaneous coronary intervention (PCI) in patients with coronary heart disease (CHD). Methods: A total of 108 patients with coronary heart disease underwent PCI in our hospital from January 2016 to January 2019 were enrolled. Coronary angiography was performed 3 to 12 months after surgery. Patients were divided into ISR group according to angiographic results. There were 17 cases and 91 cases of non-ISR group. Blood lipid levels [total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C)] were measured before PCI and before coronary angiography. The levels of RDW and ET-1 were measured before coronary angiography, and the relationship between serum lipid levels, RDW and ET-1 and ISR in patients with CHD after PCI was analyzed. Results: The levels of TC, TG, and LDL-C before coronary angiography in the ISR group were significantly higher than those in the non-ISR group (P<0.05). The levels of RDW and ET-1 in the ISR group were significantly higher than those in the non-ISR group. The differences were statistically significant (P<0.05). Logistic multivariate regression analysis results showed that TC did not decrease and LDL-C did not meet the target (controlled at 1.8 mmoL/L before coronary angiography) were risk factors for postoperative ISR in patients with coronary heart disease (P<0.05). TG did not decrease and HDL -C is not a risk factor for postoperative ISR in patients with coronary heart disease (P﹥0.05); RDW and ET-1 are risk factors for postoperative ISR in patients with coronary heart disease (P<0.05). Conclusion: TC, LDL-C, RDW and ET-1 are the high risk factors of ISR in patients with CHD after PCI. The detection of TC, LDL-C, RDW and ET-1 is helpful to evaluate the risk of ISR. 2020 Vol. 26 (4): 545-549 [Abstract] ( 134 ) HTML (1 KB)  PDF (1208 KB)  ( 348 ) 549 Expression Relationship and Significance of miR-210 and HIF-1α in Endometrial Polyps LING Ling, XU Guiqin, NI Na DOI: 10.3969/j.issn.1006-6233.2020.04.006 Objective: To observe the expressions of microRNA-210 (miR-210) and hypoxia inducible factor-1α (HIF-1α) in endometrial polyps (EP) and their clinical significances. Methods: From June 2018 to May 2019, 62 patients with endometrial polyps diagnosed by hysteroscopy and resected in our hospital were selected as the study subjects, called polyps group, and 62 normal endometrial patients with infertility or uterine malformations who underwent hysteroscopy and excluded EP during the same period were selected as the control study, called normal endometrium group. The levels of prolactin (PRL), estradiol (E2), testosterone (T), follicle stimulating hormone (FSH) and progesterone (P) were measured by enzyme-linked immunosorbent assay (ELISA), the relative expression levels of miR-210, HIF-1α, estrogen receptor (ER), progesterone receptor (PR) and vascular endothelial growth factor (VEGF) were detected by real-time fluorescence quantitative analysis (qRT-PCR), Pearson method was used to analyze the correlation between the expression levels of miR-210, HIF-1α and ER, PR, VEGF, and the expression levels of miR-210 and HIF-1α in EP tissues. Results: There was no significant difference in serum PRL, E2, T, FSH and P levels between EP patients and normal endometrium (P>0.05). The expression levels of miR-210, HIF-1α, ER and VEGF in polyps were significantly higher than those in normal intima group (P<0.05), and the expression levels of PR were significantly lower than those in normal intima group (P<0.05). MiR-210 was positively correlated with the expression of VEGF (P<0.05), HIF-1α was positively correlated with the expressions of ER and VEGF (P<0.05), and negatively correlated with the expression of PR (P<0.05). The expression level of miR-210 was positively correlated with HIF-1α (P<0.05). Conclusions: The expression of mir-210 and HIF-1 α in endometrial polyps of EP patients was up-regulated, and there was a positive correlation between them. 2020 Vol. 26 (4): 549-553 [Abstract] ( 143 ) HTML (1 KB)  PDF (1259 KB)  ( 270 ) 553 Effect of Oxygen Driven Atomization Inhalation Combined with Ventilator on Acute Heart Failure and the Influence of Blood Gas Index and Inflammatory Factors HUANG Zhengzhuang, YAN Huaixing, LI Yaofei DOI: 10.3969/j.issn.1006-6233.2020.04.007 Objective: To study the rescue effect oxygen-driven nebulization inhalation combined with ventilator on blood gas index and inflammatory factors in patients with acute heart failure. Methods: 65 patients of chronic renal failure were divided into 35 cases in the observation group, from January 2016 to January 2019 in our hospital. The 30 cases in the control group by the simple random number table, On the basis of routine treatment, the control group was treated with non-invasive ventilator, while the observation group was combined with oxygen-driven nebulization inhalation, they continuous treatment for 7 days. The clinical efficacy, the changes of blood gas index, inflammatory factors, cardiac function index and adverse reactions were compared between the two groups. Results: After treatment, the total effective rate of the observation group was significantly higher than the control group (P＜0.05). The blood gas indexe arterial partial pressure of carbon dioxide (PaCO2), arterial partial pressure of oxygen (PaO2), arterial oxygen saturation (SaO2), inflammatory factor high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), cardiac function index N-terminal pro-brain natriuretic peptide (NT-proBNP), heart rate (HR), stroke volume (SV), left ventricular ejection fraction (LVEF) of the two groups, there were significant differences between groups and at interblock and different time points(P＜0.05); The above indicators have interaction among interblock and different time points(P＜0.05); The difference before and after treatment in the observation group was greater than the control group (P＜0.05); There was no significant difference in adverse reactions between the two groups(P＞0.05). Conclusion: Oxygen-driven nebulization inhalation combined with ventilator is well for acute heart failure, which can effectively improveimprove the expression of blood gas indicators and inflammatory factors, promote the recovery of cardiac function, and has good safety, it's worthy of application and promotion. 2020 Vol. 26 (4): 553-558 [Abstract] ( 147 ) HTML (1 KB)  PDF (2329 KB)  ( 389 ) 558 Evaluation of Diagnostic Value of Carotid Ultrasound Combined with TCD in H-type Hypertension Based on Hemodynamic Parameters HUANG Haifen, LI Ren, ZHANG Wenlong DOI: 10.3969/j.issn.1006-6233.2020.04.008 Objective: To investigate the diagnostic value of carotid ultrasound (CAU) combined with transcranial doppler (TCD) to obtain hemodynamic parameters for H-type hypertension. Methods: 108 patients with H-type hypertension (observation group) and 124 patients with simple hypertension (control group) were selected. The average blood flow velocity (Vm), resistance index (RI) and pulsation index (PI) of common carotid artery, middle cerebral artery and vertebral artery were obtained by CAU and TCD.To analyze the value of hemodynamic parameters obtained by CAU combined with TCD in the diagnosis of H-type hypertension. Results: Vm of common carotid artery, middle cerebral artery and vertebral artery in the observation group was lower than that in the control group (P<0.05), RI and PI were higher than those in the control group (P<0.05).Multivariate logistic regression analysis showed that common carotid artery RI and middle cerebral artery RI were independently correlated with type H hypertension (P<0.001).The area under curve (AUC) of common carotid artery RI, middle cerebral artery RI and common carotid artery RI combined with middle cerebral artery RI in diagnosing H-type hypertension was 0.770, 0.677 and 0.850, respectively. Conclusion: Hemodynamic abnormalities in patients with H-type hypertension are more obvious than those in patients with simple hypertension. The hemodynamic parameters obtained by CAU combined with TCD have higher diagnostic value for H-type hypertension. 2020 Vol. 26 (4): 558-563 [Abstract] ( 100 ) HTML (1 KB)  PDF (1279 KB)  ( 279 ) 563 Effects of Momordica Saponin on Proliferation Apoptosis and JAK / STAT5 Pathway of Human Malignant Melanoma Skmel-2 Cells HUANG Cuimin, YE Xiuqun, LUO Yueji DOI: 10.3969/j.issn.1006-6233.2020.04.009 Objective: To investigate the effects of balsam pear saponins on the growth of human malignant melanoma SKMEL-2 cells and JAK/STAT5 pathway. Methods: Cultured human malignant melanoma SKMEL-2 cells were treated with balsam pear saponins at concentrations of 1, 2.5, 5, 7.5 and 10 mg/mL. The proliferation of SKMEL-2 cells was detected by CCK-8 method after 24, 48 and 72 hours, respectively, and the appropriate concentration and time of drug action were screened. SKMEL-2 cells cultured in vitro were randomly divided into three groups: control group, balsam pear saponin group and cisplatin group. After drug treatment, CCK-8 was used to detect cell proliferation, cell viability was measured, and cell apoptosis was detected by flow cytometry, the apoptotic rate was measured. The expression levels of caspase 3, Bcl-2, Bax, JAK, p-JAK, STAT5 and p-STAT5 were detected by immunoblotting (WB). Results: The inhibitory effect of the drug was higher at 48 hours and 72 hours than that at 24 hours, while the inhibitory effect was basically the same at 48 hours and 72 hours. The proliferation of SKMEL-2 was inhibited by balsam pear saponins at concentrations of 1-5, 7.5 and 10 mg/mL, and the inhibition increased with the increase of concentration, but after 48 and 72 hours, the inhibitory effect tended to be stable when the concentration reached 5 mg/mL. SKMEL-2 cells were treated with balsam pear saponins at a concentration of 5 mg/mL for 48 hours. Compared with control group, the relative survival rate, expressions of p-JAK, p-STAT5 and Bcl-2 in balsam pear saponin group and cisplatin group decreased significantly (P<0.05), apoptotic rate, caspase 3 and Bax expression were significantly increased (P<0.05), there was no significant difference in JAK and STAT5 expressions (P>0.05). There was no significant change in each index between balsam pear saponin group and cisplatin group (P>0.05). Conclusion: Momordica charantia saponins can inhibit the growth of SKMEL-2 cells, promote their apoptosis. It may be the mechanism that momordica charantia saponins can down-regulate the activity of JAK/STAT5 pathway, up-regulate the expressions of caspase 3 and Bax, and inhibit the expression of Bcl-2. 2020 Vol. 26 (4): 563-568 [Abstract] ( 93 ) HTML (1 KB)  PDF (1877 KB)  ( 290 ) 568 Effects of Simvastatin on Vascular Endothelial Function and Hemorheology in Patients with Coronary Heart Disease and Angina Pectoris LV Dan, JIN Dapeng, WANG Wenfeng, et al DOI: 10.3969/j.issn.1006-6233.2020.04.010 Objective: To investigate the effects of simvastatin on vascular endothelial function and hemorheology in patients with coronary heart disease and angina pectoris. Methods: 84 patients with coronary heart disease and angina pectoris admitted to our hospital from January 2015 to February 2017 were selected. The patients were divided into experimental group and control group by random number table method, 42 cases in each group. Patients in the control group were treated with routine regimen, while patients in the experimental group were treated with simvastatin on the basis of routine regimen. The vascular endothelial function and hemorheological parameters were compared between the two groups. Results: After treatment, the frequency and duration of angina pectoris attacks in the experimental group were less than those in the control group (3.14 (+0.32) times/week and (3.05 (+0.46) times/min), CK-MB (32.15 (+5.32) ug/L), LDH (147.21 (+13.22) ug/L), CRP (4.02 (+0.59) mg/L and FIB (3.11 (+0.52) g/L levels were lower than those in the control group, and there were significant differences between the two groups. (P<0.05). The levels of ET (55.42 + 2.92) ng/L and TXB2 (93.23 + 6.26) ng/L in the experimental group were significantly lower than those in the control group after treatment, with a significant difference between the two groups (P<0.05). The whole blood viscosity, plasma viscosity, hematocrit and fibrinogen levels in the experimental group were significantly lower than those in the control group (P<0.05). Conclusion: Simvastatin can effectively improve vascular endothelial function and hemorheological parameters in patients with coronary heart disease and angina pectoris. It is effective and worth recommending. 2020 Vol. 26 (4): 568-571 [Abstract] ( 110 ) HTML (1 KB)  PDF (1189 KB)  ( 242 ) 571 The Mechanism of Cucurbitacin D inhibits Proliferation and Migration of SGC-7901 Gastric Cancer Cells by Down-regulating GATA6 Expression FENG Xiaobo, LI Minhong, SHANG Jingjuan, et al DOI: 10.3969/j.issn.1006-6233.2020.04.011 Objective: To investigate the mechanism of cucurbitacin D inhibits proliferation and migration of SGC-7901 gastric cancer cells by down-regulating GATA6 expression. Methods: SGC-7901 cells were cultured in vitro. The inhibition rate of cucurbitacin D on SGC-7901 cells was detected by MTT method and the intervention dose of cucurbitacin D was determined. The cells were divided into control group, cisplatin group, cucurbitacin D low, medium and high dose groups. The control group was not treated. The cisplatin group was given cisplatin with a final concentration of 10.0 μmoL/L. The low, medium and high dose groups were given cucurbitacin D with the final concentration of 2.0, 4.0, 8.0 μmoL/L, respectively. The migration ability of SGC-7901 cells, the expression level of mi-R143 and GATA6 in SGC-7901 cells and the apoptosis and cell cycle were detected after 48 hours of culture. Results: With the increase of cucurbitacin D dose, the inhibition rate of SGC-7901 cells was significantly increased (P<0.05). When the dose of cucurbitacin was 8.0μmoL/L, the inhibition rate was 53.07%. Therefore, 8.0μmoL/L was chosen as the highest intervention dose, and 2.0μmoL/L and 4.0μmoL/L were set as two doses. Compared with the control group, the migration ability of SGC-7901 cells, the relative expression of GATA6 mRNA, and the proportion of G0 / G1 cells in the cisplatin group were significantly decreased, and the relative expression of miRNA-143 mRNA, the apoptosis rate, and the proportion of S-phase cells significantly increased(P<0.05). After treatment with cucurbitin D, the migration ability of SGC-7901 cells, the relative expression of GATA6 mRNA, and the ratio of G0 / G1 cells were significantly decreased, and the relative expression of miRNA-143 mRNA, the rate of apoptosis, and the proportion of S cells significantly increased with dose-dependent (P<0.05), but the effect was not as good as the cisplatin group(P<0.05). Conclusion: Cucurbitacin D can inhibit the proliferation and migration of SGC-7901 gastric cancer cells, and has certain anti-cancer activity. The mechanism may be related to the down-regulation of GATA6 expression by cucurbitacin D. 2020 Vol. 26 (4): 571-576 [Abstract] ( 109 ) HTML (1 KB)  PDF (1553 KB)  ( 265 ) 576 Effects of Fasudil on Cardiac Function and BNP hs-CRP ET-1 and ANP in Patients with Heart Failure CHEN Hongmei, LI Shaosheng DOI: 10.3969/j.issn.1006-6233.2020.04.012 Objective: To explore the effects of fasudil on cardiac function and B-type diuretic natriuretic peptide (BNP), high-sensitivity c-reactive protein (hs-CRP), plasma endothelin-1 (ET-1) and plasma natriuretic peptide (ANP) in patients with heart failure. Methods: 78 patients with heart failure diagnosed and treated in our hospital from September 2018 to September 2019 were selected as the study objects. They were randomly divided into study group and control group, respectively, with 39 patients. In the study group, patients were treated with fasudil, and patients in the control group were treated with conventional treatment. The differences of cardiac function, BNP, hs-crp, et-1 and ANP between the two groups were compared. Results: The NYHA of the two groups were classified as grade III and grade IV before treatment, and grade I and grade II after treatment. There was no significant difference between the two groups (P>0.05). The time of repeated measurement showed the difference before and after treatment was statistically significant (P<0.05). Repeated measurement variance showed that the time point, inter group, and inter group interaction effects of LVEF, LVEDd, LVESd, BNP, hs-CRP, ET-1, ANP in the two groups were statistically significant (P<0.05), and 1, 2, and 3 months after treatment, the level of LVEF in the study patients was higher than that in the control group (P<0.05), and the levels of LVEDd, LVESd, BNP, hs-CRP, ET-1, and ANP were lower than those in the control group Significance (P<0.05). Conclusion: The application of fasudil in patients with heart failure can effectively improve the cardiac function of patients, and has positive significance in reducing the levels of BNP, hs-crp, et-1 and ANP in patients, which is worthy of clinical promotion. 2020 Vol. 26 (4): 576-580 [Abstract] ( 108 ) HTML (1 KB)  PDF (1193 KB)  ( 325 ) 581 Effects of Remote Ischemia on Serum Cardiac Troponin and Systemic Inflammatory Response in Patients Undergoing Cardiac Valve Replacement ZHUANG Huanwei, BAI Shutang, FU Hongdu, et al DOI: 10.3969/j.issn.1006-6233.2020.04.013 Objective: To investigate the effects of remote ischemic treatment (RIPC) on serum cardiac troponin I (cTnI) and systemic inflammatory response in patients undergoing cardiac valve replacement. Methods: A total of 66 patients who underwent cardiac valve replacement in our hospital from August 2017 to August 2019 were selected as the study subjects. They were divided into study group and control group according to the random number table method, with 33 cases in each group. Patients in the study group were treated with RIPC after anesthesia induction, and patients in the control group were treated with conventional tourniquet. The effects of different treatments on CTnI and inflammatory factors (TNF-α,IL-6,IL-8) were compared between the two groups. Results: CTnI concentration in the two groups increased gradually with time after aortic dissection (P<0.05), and the concentration of cTnI in the study group was lower than that in the control group (P<0.05).At the same time, the BNP level of the patients in the two groups was gradually decreased (P<0.05) until 6h before anesthesia induction, and increased rapidly after 24h of aorta opening (P<0.05). However, the BNP level in the study group was lower than that in the control group (P<0.05) after 30min, 6h and 24h of aorta opening. In the other two groups.the levels of TNF-α,IL-6,IL-8 increased at one day after opereation (P<0.05), but the levels of TNF-α,IL-6,IL-8 at 3 and 7 days after operation were showed a dounwardtreat (P<0.05). and the level of TNF-α,IL-6,IL-8 in paeitns in this study group were louer than those inthe control group at 3 and 7 days after surgery (P<0.05).Conclusion: The treatment of distant ischemia can effectively reduce the myocardial ischemia-reperfusion injury, play a protective role in valve replacement surgery, and has a positive significance for the reduction of inflammatory response in patients. 2020 Vol. 26 (4): 581-585 [Abstract] ( 81 ) HTML (1 KB)  PDF (1199 KB)  ( 259 ) 585 APOE Gene Expression in Elderly Patients with AD at Different Age Stages and its Relationship with Cognitive Impairment ZHU Ye, CHEN Yan, PENG Le, et al DOI: 10.3969/j.issn.1006-6233.2020.04.014 Objective: To investigate the expression of apolipoprotein E (APOE) gene in elderly patients with Alzheimer's disease (AD) at different ages and stages, and to analyze the relationship between APOE gene expression and cognitive impairment. Methods: 64 elderly AD patients admitted to our hospital from June 2018 to June 2019 were selected as the main subjects. According to the age of onset, the patients were divided into early-onset AD group (<65 years old) and late-onset AD group (>65 years old). The late-onset AD group was further divided into old-onset group (65-80 years old), old-onset group (80~90 years old) and longevity Group (>90 years old), with 16 cases in each group. The APOE gene expression in peripheral blood of four groups was detected, and the correlation between APOE gene expression and cognitive impairment was analyzed by Pearson correlation. Results: E2/E2 APOE subtypes were not present in all 4 groups. In E3/E3 subtypes, the frequency of group 1 was the highest, 62.50% (10/16), followed by 43.75% of the 2 groups (7/16), in the E3/E4 subtype expression, the frequency of the three groups was the highest, 43.75% (7/16), followed by 37.50% (6/16) of the 4 groups, and the expression of the other subtypes was lower; APOE There are three E1/E3/E4 alleles, of which E3 has the highest frequency of expression, followed by E4, and E2 has the lowest frequency. The MMSE, ADAS-cog, and ADL scores of the 4 groups were statistically significant (P<0.05), and the MMSE scores were: 1 group>2 groups>3 groups>4 groups, and the ADAS-cog score:1 group<2 group<3 group<4 group, ADL score:1 group<2 group<3 group<4 group, the difference was statistically significant (P<0.05). The MMSE score, ADAS-cog score, and ADL score of patients with different APOE genotypes were statistically significant (P<0.05). There was no significant difference in LSD-t test between the two groups (P>0.05). Conclusion: The expression of APOE gene is different in AD patients with different ages, and the expression of APOE4 gene is closely related to cognitive dysfunction. Therefore, the age of AD can be delayed by regulating the expression of APOE4 gene. 2020 Vol. 26 (4): 585-589 [Abstract] ( 93 ) HTML (1 KB)  PDF (1209 KB)  ( 263 ) 589 Correlation between Serum Uric Acid with Artery Calcification in Patients with Chronic Kidney Disease ZHOU Long, HU Chunlan, WU Zecheng , et al DOI: 10.3969/j.issn.1006-6233.2020.04.015 Objective: To investigate the correlation between serum uric acid with coronary artery calcification in patients with chronic kidney disease (CKD). Methods: 142 patients with CKD hospitalized in Chongqing Three Gorges Central Hospital from April 2016 to January 2019 were selected as the research subjects. The arterial calcification was monitored by X-ray at admission. And Simultaneous determination of serum uric acid (UA), urea nitrogen (BUN), serum creatinine (SCr), serum calcium and other blood biochemical indicators. According to the results of coronary artery calcification, the patients were divided into calcification group (66 cases) and non-calcification group (76 cases), Arterial calcification scores and UA levels after hemodialysis were measured, the relationship between Arterial calcification scores with UA and the risk factors of coronary artery calcification were analyzed. Results: The TG, CRP, 24h urinary protein, proportion of hypertension and chronic nephritis history in calcification group were significantly higher than those in non-calcification group (P<0.05), while Hb and eGFR were lower than those in non-calcification group (P<0.05); the UA level before dialysis in calcification group was higher than that in non-calcification group (P<0.05), and the calcification scores and UA level after dialysis were lower than those before dialysis (P<0.05); there was a positive correlation between Arterial calcification scores with UA level in CKD patients (P<0.05); UA and eGFR were risk factors for coronary artery calcification in CKD patients. Conclusion: Serum uric acid can aggravate arterial calcification in patients with CKD. Early intervention of uric acid therapy and adequate dialysis may be an effective way to improve arterial calcification. 2020 Vol. 26 (4): 589-594 [Abstract] ( 124 ) HTML (1 KB)  PDF (2366 KB)  ( 467 ) 594 Effect of Mechanical Thrombectomy Combined with Catheter Thrombolysis on Coagulation Function, Recurrence and Amputation in Patients with Acute Lower Extremity Arterial Embolism WU Ligang, ZENG Hanqiang DOI: 10.3969/j.issn.1006-6233.2020.04.016 Objective: To analyze the effects of mechanical thrombectomy combined with catheter thrombolysis on coagulation function, recurrence and amputation in patients with acute lower extremity arterial embolism. Methods: 84 patients with acute lower extremity arterial embolism in our hospital from January 2016 to August 2018 were randomly divided into study group and control group, 42 cases in each group. The control group was treated with mechanical thrombectomy, and the study group was treated with catheter thrombolysis on this basis. After treatment, the clinical treatment and coagulation function [including prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB), D-Dimer (D-D)] of the two groups were compared, and the amputation rate and recurrence rate of the two groups were compared. Results: After treatment, the total effective rate of the study group was significantly higher than that of the control group (P<0.05). The levels of FIB and D-D in the two groups were significantly lower than those before treatment, and PT and APT were significantly higher than those before treatment (P<0.05); the levels of FIB and D-D in the study group were significantly lower than those in the control group, and PT and APT were significantly higher than those in the control group (P<0.05). The recurrence rate of the study group was significantly lower than that of the control group (P<0.05), but there was no significant difference in amputation rate between the two groups (P>0.05). Conclusion: Mechanical thrombectomy combined with catheter thrombolysis can significantly improve the coagulation function of patients with acute lower extremity arterial embolism, better reduce the recurrence rate, lower the risk of amputation, clinical efficacy is clear. 2020 Vol. 26 (4): 594-597 [Abstract] ( 80 ) HTML (1 KB)  PDF (1186 KB)  ( 282 ) 598 Comparative Study between Full-screen Digital Mammography and Digital Breast Tomography in the Diagnosis of Breast Cancer with Dense Breast and Calcification of Breast QIN Jinxia, ZHAO Yunian, WANG Huihua, et al DOI: 10.3969/j.issn.1006-6233.2020.04.017 Objective: To investigate the diagnosis of breast cancer and the calcification of breast calcification in full-screen digital mammography (FFDM) and digital breast tomography (DBT). Methods: A total of 156 patients with dense breast adenocarcinoma who came to our hospital from March 2017 to March 2018 were enrolled. All patients were diagnosed by DBT and FFDM, and the breast gland density classification, lesion morphology, size, quantity and boundary were analyzed and recorded. The pathological diagnosis was used as the gold standard to evaluate the compactness of the two examination methods. The detection rate and focus characteristics of breast cancer in dense breast by two methods were evaluated. Results: A total of 157 lesions were detected in 156 patients, all of which were breast cancer. One of them was bilateral breast cancer. Using pathological examination as a standard, in 156 patients, 51 patients (32.69%) had breast cancer glandular tissue with high uniformity and dense breast cancer, and 105 patients (67.31%) had uneven and dense breast cancer. The diagnostic accuracy of DBT for dense breast breast masses and calcifications in breasts (78.34%, 54.14%) was significantly higher than that of FFDM (56.69%, 35.67%) (P<0.05). Moreover, the total diagnostic accuracy of DBT for dense mammary gland (82.80%) was significantly higher than that of FFDM (67.53%) (P<0.05). DBT showed a better overall effect on the boundary of dense breast lesions than FFDM (Z=-9.196, P<0.05). DBT showed significantly better burr signs and vascular penetration signs in dense breast cancer than FFDM (P<0.05). There was no significant difference in the total detection rate of breast cancer calcification in dense breasts between the two methods (χ2=2.136, P>0.05). Conclusion: Compared with FFDM, DBT is more conducive to the characteristics of lesions in patients with calcification, and is more suitable for the diagnosis and calcification of breast cancer in dense breast. 2020 Vol. 26 (4): 598-602 [Abstract] ( 121 ) HTML (1 KB)  PDF (1198 KB)  ( 290 ) 602 Clinical Efficacy of Calcitriol Combined with Metformin on Early Diabetic Nephropathy in Type 2 Diabetes Mellitus Patients with Abdominal Obesity CHEN Jiaoyue, SHAN Xiujie, LIU Min, et al DOI: 10.3969/j.issn.1006-6233.2020.04.018 Objective: To investigate the clinical efficacy and mechanism of calcitriol combined with metformin in the treatment of type 2 diabetes mellitus(T2DM) patients with abdominal obesity and early diabetic nephropathy(EDN).Methods: 80 T2DM patients with abdominal obesity and EDN were selected and divided into observation group and control group according to the random number table method, 40 patients in each group.The observation group was treated with calcitriol combined with metformin, while the control group was treated with metformin. Waistline, body mass index, fasting plasma glucose, calcium, phosphorus,glycosylated hemoglobin(HbAlc), leptin(LP), adiponectin(ADP), urinary microalbumin/creatinine (ACR) and 25-(OH)D3 were compared between the two groups before and after treatment. Pearson corelation analysis was used to analyze the correlation between serum 25-(OH)D3 level and ACR. Results: Waistline, BMI, FPG, HbAlc, LP and ACR were significantly decreased in the two groups after treatment, and 25-(OH)D3 and ADP were significantly increased(P<0.05).Compared with the control group, waistline, BMI, FPG , HbAlc, LP and ACR were significantly decreased, while 25-(OH)D3 and ADP were significantly increased in the observation group(P<0.05). Conclusion: Early combination of calcitriol and metformin in T2DM patients with abdominal obesity can increase the level of 25-(OH)D3 and reduce urinary protein, which may be related to the regulation of ADP and LP levels。 2020 Vol. 26 (4): 602-605 [Abstract] ( 161 ) HTML (1 KB)  PDF (1191 KB)  ( 277 ) 605 Correlation between APACHE II Score and Delirium Occurrence in ICU Patients with Spinal Cord Injury Undergoing Invasive Mechanical Ventilation LI Xiaoya, et al DOI: 10.3969/j.issn.1006-6233.2020.04.019 Objective: To investigate the predictive value of acute physiology and chronic health status scoring system II (APACHE II) in patients with cervical spinal cord injury undergoing invasive mechanical ventilation (MV) in intensive care unit (ICU). Methods: A total of 76 patients with spinal cord injury on mechanical ventilation was enrolled in the study. The APACHE II scores of each patient within 24 hours of admission were collected. Each patient was assessed using Confusion Assessment Method for the ICU (CAM-ICU), and the patients were divided into delirium group (n=30) and non-delirium group (n=46) according to the evaluation results. The APACHE II score, MV time, and ICU hospitalization time of the two groups were analyzed with statistical methods,The 76 enrolled patients were further divided into 5 groups according to their APACHE II scores, with an increment of 5. The correlation between the APACHE II score and the incidence of delirium was analyzed for all patients. The receiver operating characteristic curve (ROC) was plotted, the relevant indicators for predicting delirium occurrence were calculated, and the value of APACHE II score in predicting delirium occurrence was assessed. Results: Among 76 patients with spinal cord injury who underwent MV, the incidence of delirium was 39.47% (30/76). The APACHE II score of the delirium group was higher than that of the non- delirium group (26.63±4.27 vs 19.37±4.23, P<0.05). The MV time of the delirium group was longer than that of the non- delirium group, which was statistically different (15.87±4.58 vs11. 80±4.74, P<0.05); the ICU in hospitalization time in the delirium group was longer than that in the non- delirium group, the difference was statistically significant (18.63±5.87 vs 14.11±5.28, P<0.05). The incidence of delirium was linearly related to the APACHE II score. The ROC curve analysis showed that the area under the curve (AUC) of the APACHE II score predicted delirium was 0.884, and the best intercept point for delirium prediction was when APACHE II score>23, with sensitivity of 76.67% and specificity of 84.78%. Conclusion: The incidence of delirium in patients with spinal cord injury undergoing mechanical ventilation increased with the increase of APACHE II score, which has a high predictive value for the occurrence of delirium in such patients. 2020 Vol. 26 (4): 605-609 [Abstract] ( 96 ) HTML (1 KB)  PDF (1424 KB)  ( 239 ) 609 Effects of Probucol on Hemorheology and Coagulation Related Indexes in Patients with Cerebral Infarction ZHANG Xiaojie, GUO Aihong, LI Yanjun DOI: 10.3969/j.issn.1006-6233.2020.04.020 Objective: To study the effect of probucol on Hemorheology and coagulation in patients with cerebral infarction. Methods: 86 patients with cerebral infarction admitted to our hospital from December 2017 to December 2018 were included in the study. They were randomly divided into two groups, 43 in each group. The control group was given routine basic treatment, while the observation group was given probucol on the premise of basic treatment, which lasted for 4 weeks. The changes of plasma viscosity, whole blood high shear viscosity, whole blood low shear viscosity, activated partial thromboplastin time, thrombin time and fibrinogen were compared before and after treatment. The changes of hypersensitive C-reactive protein and matrix metalloproteinase-9 before and after treatment were analyzed, and the adverse reactions during treatment were observed. Results: The total effective rate was 90.70% in the observation group and 74.42% in the control group (P<0.05). The plasma viscosity, whole blood high shear viscosity and whole blood low shear viscosity in the observation group were significantly lower than those before treatment and after treatment in the control group (P<0.05), and the difference of the above indexes before and after treatment was significantly lower than that in the control group (P < 0.05). After treatment, the activated partial thromboplastin time and thrombin time in the observation group were significantly higher than those before treatment and after treatment in the control group (P < 0.05), and the difference of the above indexes before and after treatment was significantly higher than that in the control group (P<0.05); after treatment, the fibrinogen in the observation group was significantly lower than that before and after treatment in the control group (P < 0.05), and the difference before and after treatment was significantly lower than that in the control group (P<0.05). After treatment, the levels of serum high-sensitivity C-reactive protein and matrix metalloproteinase-9 in the observation group were significantly lower than those before and after treatment in the control group (P<0.05), and the differences of the above indexes before and after treatment were significantly lower than those in the control group (P < 0.05). The incidence of adverse reactions was 11.63% in the observation group and 18.60% in the control group (P>0.05). Conclusion: Probucol is effective in the treatment of cerebral infarction. It can effectively reduce plasma viscosity, whole blood high shear viscosity, whole blood low shear viscosity, improve hemorheology and coagulation function, reduce inflammation after cerebral infarction, and has good safety. 2020 Vol. 26 (4): 609-615 [Abstract] ( 102 ) HTML (1 KB)  PDF (1242 KB)  ( 246 ) 615 The Reference Value of Quadruple Screening Down's Syndrome in the Second Trimester ZHU Yanju, KAN Changli, XIAO Yanping, et al DOI: 10.3969/j.issn.1006-6233.2020.04.021 Objective:To explore the reference value of Down's syndrome screening with AFP, HCG, UE3 and inhibin A in the second trimester of pregnancy, so as to provide theoretical and practical basis for predicting the risk rate of pregnant women and children with Down's syndrome and for further screening and diagnosis and treatment according to the risk rate.Methods: A retrospective analysis was made of 201 singleton pregnant women (7 fetuses with Down's syndrome) who were successfully screened and followed up in our hospital from March 2017 to March 2018. To detect HCG,AFPand UE3 、inhibin A . thethree-and four-way screening of Down's syndrome risk values were calculated by suss related parameters, and the effect of different cut value levels on Down's syndrome screening was analyzed.Result: In 201 subjects, the total abnormal rate of mom in different age groups was 24.88%, in which the abnormal rates of HCG, AFP, uE3 and inhibin A were 8.96%, 9.95%, 1.99% and 3.98% respectively. There were 7 cases (3.48%) of chromosomal abnormalities in amniocentesis. Among them, 5 cases (71.43%) had abnormal mom value ≥1. The incidence of chromosomal abnormalities in normal mom was 1.32% (2/151) which was 10.00% (5/50) higher than that in abnormal mom (P<0.05).the SURSS calculation showed that the false-positive rate of AFP+uE3+HCG+Inhibin A was 3.12%, 5.75% and 7.68% respectively, which was 0.85%, 2.01% and 3.52% lower than that of triple screening. The false positive rate of quadruple screening was significantly lower than that of double screening and single screening (P<0.05). Pearson's score showed that Inhibin A was positively correlated with HCG and AFP in normal pregnant women, but negatively correlated with HCG and AFP in Down's syndrome pregnant women (P<0.05),There was no correlation between inhibin A and uE3 (P > 0.05).Conclusion:In the mid pregnancy screening of Down's syndrome, scientific and reasonable HCG+AFP+uE3+inhibin A quadruple screening is more effective and has good clinical application and promotion value. 2020 Vol. 26 (4): 615-619 [Abstract] ( 195 ) HTML (1 KB)  PDF (1206 KB)  ( 468 ) 620 Meta Analysis of the Efficacy and Safety of Docetaxel Combined with Cisplatin for Advanced Non-small Cell Lung Cancer GENG Shuai, GUO Hongju DOI: 10.3969/j.issn.1006-6233.2020.04.022 Objective: To explore the efficacy and safety of docetaxel combined with cisplatin in the treatment of advanced non-small cell lung cancer (NSCLC), and to provide theoretical basis for clinical treatment. Methods: The databases such as Cochrane Library, EMBASE, PubMed, CBM, CNKI, etc. were searched on the Internet. The RCTs of docetaxel and cisplatin in the treatment of advanced NSCLC were searched from the establishment to September 2019. The data of RCTs were integrated and checked by two researchers and analyzed by Revman 5.2 software. Results: In this paper, 14 RCTs (1199 patients) were selected, including 611 in the treatment group and 588 in the control group. In terms of effectiveness, the total effective rate and clinical benefit rate of docetaxel and cisplatin group were significantly different, the total effective rate RR (95% CI) was 1.56 (1.36, 1.80); the clinical benefit rate RR (95% CI) was 1.13 (1.07, 1.19); in terms of safety, the risk of adverse reactions in the treatment group was significantly lower than that in the control group, with leukopenia and thrombocytopenia RR (95% CI) was 0.69 (0.57, 0.83), 0.44 (0.29, 0.65), 0.54 (0.40, 0.73), 0.49 (0.33, 0.72), respectively. Conclusion: Based on the selected evidence, the treatment group was superior to the control group in the treatment of advanced NSCLC. 2020 Vol. 26 (4): 620-625 [Abstract] ( 109 ) HTML (1 KB)  PDF (1685 KB)  ( 317 ) 625 Clinical Analysis of the Relationship between Serum S-100 β and Sleep Disorder and Cognitive Function in the Elderly Patients with Acute Cerebral Infarction FENG Hao, LIU Wei, TANG Xiaomei DOI: 10.3969/j.issn.1006-6233.2020.04.023 Objective: To study the relationship between serum S-100 β and sleep disorder and cognitive function sleep in elderly patients with acute cerebral infarction. Methods: 216 elderly patients with acute cerebral infarction admitted to our hospital from May 2015 to May 2019 were divided into group A (with non-rapid eye movement sleep disorder, 112 cases) and group B (without non-rapid eye movement sleep disorder, 104 cases).All patients accept guide more sleep monitoring, compared two groups of total sleep time (TST), sleep latency (SOL), number of awakening (WUT), sleep efficiency (SE) and rapid eye movement (REM) sleep period (REMT), REM sleep time period of total sleep time percentage(REM%) sleep and non-rem sleep (NREM) sleep stage S1, S2, S3 + S4 sleep time.Montreal Cognitive Assessment Scale (MOCA) was used to assess the cognitive function of the two groups. Serum S-100beta level and its relationship with sleep parameters and cognitive function were analyzed. Results: The sleep time of TST, SE, REMT, REM% and NREM in group A was significantly lower than that in group B (P<0.05), while the sleep time of SOL, WUT and NREM in stage S1 was significantly higher than that in group B (P<0.05).The scores of visual space and executive function, attention, delayed memory, orientation and MOCA in group A were significantly lower than those in group B (P<0.05), but there was no significant difference in naming, language and abstract scores between group A and group B (P>0.05).Serum S-100beta level in group A was significantly lower than that in group B (P<0.05). In elderly patients with acute cerebral infarction with non-rapid eye movement sleep disorder, serum s-100 was negatively correlated with TST, SE, REMT, REM%, S2, S3+S4 (r=-0.466, -0.682, -0.612, -0.646, all P<0.05), positively correlated with SOL, WUT, and S1 (r=0.435, 0.587, 0.187, all P<0.05), and negatively correlated with the total score of MOCA (r=-0.327, P<0.05). Conclusion: Serum S-100beta is associated with sleep quality and cognitive function in elderly patients with acute cerebral infarction and non-REM sleep disorder. 2020 Vol. 26 (4): 625-630 [Abstract] ( 118 ) HTML (1 KB)  PDF (1228 KB)  ( 260 ) 631 Analgesic Effect of Sufentanil Combined with Ropivacaine in Cesarean Section and Its Effect on Gastrointestinal Function ZHAO Yuanyuan, LI Ju, WAN Yan, et al DOI: 10.3969/j.issn.1006-6233.2020.04.024 Objective: To explore the analgesic effect of sufentanil combined with ropivacaine in cesarean section and its effect on gastrointestinal function. Methods: 60 cases of cesarean section mothers in our hospital from April 2017 to April 2018 were selected for the study,were randomly divided into the observation group (n=32) and the control group (n=28). The control group was treated with ropivacaine for analgesia, and the observation group was treated with sufentanil combined with ropivacaine for analgesia. The time of spontaneous urination after anesthesia, the time of the first movement out of bed, the effective analgesia time, the pain score at 12h, 24h and 48h after the operation, the nursing condition, and the adverse reactions of gastrointestinal function recovery time were compared between the two groups. Results: The time of spontaneous urination and the time of getting out of bed for the first time in the observation group was significantly lower than that in the control group, and the effective analgesia time was higher than that in the control group (P<0.05). The pain scores of the observation group at 12h, 24h and 48h after surgery were significantly lower than those of the control group (P<0.05). The colostrum time in the observation group was lower than that in the control group, and the number of lactation at 24h was significantly higher than that in the control group (P<0.05). After surgery, the recovery time of bowel sound, anal exhaust time and anal defecation time in the observation group were significantly lower than those in the control group (P<0.05). The total incidence of hypotension, bradycardia, nausea and vomiting in the two groups was 9.38% and 35.71%, respectively, with statistically significant differences (P<0.05). Conclusion: Sufentanil combined with ropivacaine has obvious analgesic effect on cesarean section parturients, which can effectively improve the gastrointestinal function of patients. It is safe and effective, and worthy of promotion and application. 2020 Vol. 26 (4): 631-634 [Abstract] ( 139 ) HTML (1 KB)  PDF (1177 KB)  ( 283 ) 635 Analysis of Factors Related to Vascular Access Selection and Internal Fistula Use in Patients with End-stage Renal Disease ZHAO Yanhong, LI Hongmei, YANG Chongmeng, et al DOI: 10.3969/j.issn.1006-6233.2020.04.025 Objective: To explore the selection of vascular access and the use of internal fistula in patients with end-stage renal disease. Methods: 80 ESRD patients enrolled for hemodialysis in the blood purification center of the department of nephrology of our hospital from June 2017 to January 2019 were selected as the study objects. According to the establishment of vascular access, the patients were divided into arteriovenous fistula and central venous catheter group.The clinical data of the two groups were compared and the influencing factors of vascular access selection in patients with initial hemodialysis were analyzed. Results: Of the 80 ESRD patients, 12 (15%) established AVF and 68 (85%) established CVC. Univariate analysis showed that gender, age, BMI, glomerular filtration rate and ESRD etiology of ESRD patients in the two groups had no statistically significant difference in vascular access selection (P>0.05). After 6 months of follow-up, the overall incidence of complications was 25% in the AVF group and 57.35% in the CVC group, indicating a statistically significant difference between the two groups (χ2=4.281, P<0.05). In addition, within 6 months of 80 patients undergoing maintenance dialysis, 1 patient in the AVF group was converted to kidney transplantation, and 11 patients were still treated with AVF, with a treatment conversion rate of 8.33% (1/12). In the CVC group, 48 patients were converted to AVF, 5 to peritoneal dialysis, 2 to kidney transplantation, and only 13 remained CVC, with a treatment conversion rate of 80.88% (55/68). Multivariate Logistic regression analysis showed that education level, regional distribution, residence location, and the time from the recommended fistula to the start of dialysis were all relevant influencing factors for the use of AVF(B=-2.954, 3.024, -2.479, -2.259, P均<0.05). Conclusion: Most HD patients were treated with deep vein catheter for the first time, and the use rate of AVF was very low. In addition, there are many factors influencing the use rate of AVF during the first dialysis, including education level, regional distribution, registered permanent residence location, and the recommended time from fistulation to the beginning of dialysis. 2020 Vol. 26 (4): 635-639 [Abstract] ( 95 ) HTML (1 KB)  PDF (1194 KB)  ( 287 ) 639 Clinical Study of Three Different Bone Grafting Methods in the Treatment of Lumbar Degenerative Diseases with TLIF ZHENG Yuesheng, et al DOI: 10.3969/j.issn.1006-6233.2020.04.026 Objective: To explore the clinical effect of three different bone grafting methods in the treatment of lumbar degenerative diseases by TLIF. Methods: The clinical data of 109 patients with lumbar degenerative diseases who were treated by TLIF in Guangzhou General Hospital of Guangzhou Military Region from January 2012 to January 2016 were analyzed retrospectively. According to the fusion mode, they were divided into autoiliac bone + fusion cage (group A, 42 cases), autolaminar articular process bone + fusion cage (group B, 38 cases), allogeneic bone + fusion cage (Group C, 29 cases). The patients were followed up for 3 months, 6 months and 12 months. The operative indexes, fusion rate, VAS score of pain and JOA score of lumbar vertebrae were compared among the three groups. Results: There was no significant difference in operation time, intraoperative blood loss and hospital stay among the three groups (P>0.05). Three months after operation, the fusion rate of group A was higher than that of group B and group C, with statistical difference (P<0.05). There was no statistical difference in the fusion rate of 6 and 12 months after operation (P>0.05). Before operation, there was no significant difference in VAS and JOA scores among the three groups (P>0.05). At 3, 6 and 12 months after operation, the VAS scores of the three groups were lower than that before operation, and there was statistical difference between the three groups at 3 months after operation, but there was no statistical difference between the three groups at 6 and 12 months after operation (P>0.05); at 3, 6 and 12 months after operation, the JOA scores of the three groups were higher than that before operation, and there was no statistical difference between the three groups (P>0.05), and there was statistical difference between the two groups (P<0.05). Conclusion: TLIF is an effective method for the treatment of lumbar degenerative diseases. The short-term effect of the three methods is satisfactory. The interbody fusion rate of the autogenous ilium fusion group is better than that of the autogenous laminectomy fusion group and the allogeneic bone fusion group 3 months after the operation. 2020 Vol. 26 (4): 639-644 [Abstract] ( 87 ) HTML (1 KB)  PDF (1398 KB)  ( 254 ) 644 Effects of Different INR on Thromboembolic Events and Major Adverse Cardiovascular Events in Patients with Coronary Heart Disease Complicated with Atrial Fibrillation XU Xianbin, LU Xusheng, CHEN Erzhou, et al DOI: 10.3969/j.issn.1006-6233.2020.04.027 Objective: To study the effect of INR on thromboembolic events and adverse cardiovascular events in patients with coronary heart disease and atrial fibrillation. Methods: From December 2013 to October 2018, 120 patients with coronary heart disease and atrial fibrillation who had thromboembolic events and adverse cardiovascular events were selected as the observation group, and 110 patients with coronary heart disease and atrial fibrillation who did not have thromboembolic events and adverse cardiovascular events were selected as the control group. The risk factors of thromboembolism and adverse cardiovascular events were analyzed by single factor and multi factor Logistic regression, and the distribution of INR value in the observation group was observed; the predictive value of INR for thromboembolism and adverse cardiovascular events in patients with coronary heart disease and atrial fibrillation was analyzed by ROC curve. Results: Univariate and multivariate Logistic regression analysis showed that the age of INR, left atrial enlargement ≥ 40mm, left ventricular ejection fraction < 50%, hypertension and chronic kidney disease cardiac function grade were the influencing factors and independent risk factors of cardiovascular adverse events and thrombus embolism events in patients with coronary heart disease complicated with AF (P<0.05).There were 6 cases (5.00%) with INR value < 1.5, 4 cases (3.33%) with INR value of 1.5-1.99, 20 cases (16.67%) with INR value of 2.0-2.49,90 cases (75.00%) with INR value ≥ 2.5 in the observation group. The sensitivity and specificity of INR in predicting thrombus embolism and adverse cardiovascular events in patients with coronary heart disease complicated with atrial fibrillation were 77.5%, 100.0%, 0.915 and 2.35 respectively. Conclusion: INR in patients with coronary heart disease complicated with atrial fibrillation is an independent risk factor for thrombus embolism and adverse cardiovascular events, which has certain value in predicting thrombus embolism and adverse cardiovascular events. For example, the higher the probability of thrombus embolism and adverse cardiovascular events in patients with coronary heart disease complicated with atrial fibrillation, the higher the probability of thrombus embolism and adverse cardiovascular events. 2020 Vol. 26 (4): 644-649 [Abstract] ( 93 ) HTML (1 KB)  PDF (1309 KB)  ( 458 ) 649 Clinical Study of Aldosterone Receptor Antagonists in the Prevention and Treatment of Atrial Fibrillation after Pacemaker Operation WU Youhua, LI Nan, XU Baohua DOI: 10.3969/j.issn.1006-6233.2020.04.028 Objective: To evaluate the preventive effects of spironolactone (SPI, one of the aldosterone receptor antagonists) on atrial fibrillation of patients after pacemaker implantation and impacts on left atrial diameter and cardiac function. Methods: From January 2018 to April 2019, a total of 84 patients with dual chamber pacemaker implantation were selected. Atrial fibrillation was excluded before entering the group. The patients were randomly divided into two groups: the spironolactone group (42 cases) and the control group (42 cases). The spironolactone group was treated with oral spironolactone (20mg / D) on the basis of routine treatment, and the control group was treated with routine treatment after pacemaker operation. The patients in the two groups were superceded at 1, 3, 6 and 12 months before and after operation Echocardiography (recording left atrial diameter and left ventricular ejection fraction) and serum aldosterone level were measured. Pacemakers were programmed 1, 3, 6 and 12 months after operation and the recurrence of AF was recorded. Results: After 12-month follow-up visit, there are 7 cases (16.7%) of recurrence of atrial fibrillation in spironolactone group and 15 cases (35.8%) in control group. Therefore, there are no significant difference (p=0.081). Within 12 months after operation, left atrial diameter in spironolactone group is smaller than that of control group (31.3±2.3mm vs. 33.8±2.1mm). Obviously, the difference has statistical significance (P<0.05). However, during each period, there is no significant difference with regard to left ventricular ejection fraction among two groups (P>0.05). The level of plasma aldosterone of spironolactone group is smaller than that of control group in the 3rd and 6th month after operation with significant difference (P<0.05), but the level is increasing after 6 months until there is no significant difference in 12th month (P>0.05). Conclusion: Left atrial expansion can be delayed by taking spironolactone. The mechanism is to suppress the function of aldosterone. Long-term application may reduce and delay recurrence of atrial fibrillation after pacemaker implantation. 2020 Vol. 26 (4): 649-653 [Abstract] ( 73 ) HTML (1 KB)  PDF (1197 KB)  ( 203 ) 653 Correlation between Vitamin A Deficiency and the Severity of Mycoplasma Neumoniae Neumonia in Hildren SONG Xiaotong, SUN Yingjun, ZHANG Yan DOI: 10.3969/j.issn.1006-6233.2020.04.029 Objective: To observe the correlation between vitamin A (VA) deficiency and the severity of M PP in children. Methods: 300 cases of hospitalized children diagnosed with M PP in Cangzhou People's Hospital from March tudied, and they were divided into M PP group (220 cases) and 2017 to August 2018 were s refractory M PP group (RM PP group, 80 cases) according to the degree of illness.VA and immunoglobulin levels in the two groups were compared to observe the normal detection of clinical VA deficiency (CVAD) and subclinical VA deficiency (SVAD) +VA in the two groups, and analyze the relationship between VA deficiency and co-infection and immunoglobulin levels in the children with RM PP. Results: The VA level of common MPP group was significantly higher than that of RM PP group (P<0.05), and the detection rate of CVAD and the levels of IgM, IgA and IgG were significantly lower than that of RM PP group (P<0.05). RM PP combined with infection was found in 27 cases (33.75%) and 53 cases without infection (66.25%); The detection rate of CVAD in RMPP with infection was significantly higher than that in RMPP without infection (P<0.05). The levels of IgM and IgA in CVAD group were significantly higher than those in SVAD group and VA normal group (P<0.05). Conclusion: VA deficiency is related to the severity of M PP, which should be paid enough attention in clinic. 2020 Vol. 26 (4): 653-656 [Abstract] ( 146 ) HTML (1 KB)  PDF (1195 KB)  ( 360 ) 657 Correlation between Plasma ET-1 Level and the Degree of Lower Extremity Vascular Disease in Elderly Type 2 Diabetic Patients SHOU Zhang, ZHAO Yongcai, ZHANG Yan, et al DOI: 10.3969/j.issn.1006-6233.2020.04.030 Objective: To investigate the correlation between plasma endothelin-1 (ET-1) level and the degree of lower extremity vascular disease in elderly patients with type 2 diabetes. Methods: A total of 136 elderly patients with diabetes mellitus and lower extremity vascular disease treated in our hospital from January 2015 to January 2019 were randomly selected. Based on the results of ankle brachial index (ABI) measurement in elderly patients, they were divided into a 84 patients mild lower extremity arteriosclerosis group (0.7≤ABI <0.9), a 36 patients moderate lower extremity arteriosclerosis group (0.5≤ABI <0.7), and a 16 patients severe lower extremity arteriosclerosis group (ABI <0.5),Glucose oxidase method was used to measure fasting blood glucose (FBG), chromatographic method was used to measure glycated hemoglobin (HbA1c), and automatic biochemical analyzer was used to measure the cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL), and triacylglycerol (TG), etc. The enzyme-linked adsorption method was used to measure the plasma ET-1 levels in patients among different lower extremity arteriosclerosis groups, and we analyzed the correlation between the degree of aortic lesions in different limbs and the level of plasma ET-1 . Results: There were significant differences in age, BMI, TC, TG, LDL, HDL, course of disease, blood glucose under fasting, and ET-1 level between groups with different degrees of pathological changes of lower extremity arteries in type 2 diabetes (P <0.05). Logistic regression analysis showed that, fasting blood glucose, duration of diabetes, and ET-1 are independent risk factors that affect the degree of arterial disease in the lower limbs. Spearman rank correlation analysis showed that the degree of diabetic lower extremity vascular lesions was positively correlated with ET-1 (r = 0.336, P = 0.000). Conclusion: The independent risk factors for the degree of lower extremity vascular disease in type 2 diabetes are fasting blood glucose, duration of diabetes, which are positively correlated with ET-1 level. 2020 Vol. 26 (4): 657-660 [Abstract] ( 128 ) HTML (1 KB)  PDF (1294 KB)  ( 325 ) 660 Analysis on Relationship between TBX (20) Gene Polymorphism and Congenital Heart Disease in Newborns MA Lixian DOI: 10.3969/j.issn.1006-6233.2020.04.031 Objective: To analyze the relationship between TBX_ (20) gene polymorphism and congenital heart disease in newborns. Methods: 40 neonates with congenital heart disease from August 2016 to August 2018 were randomly selected as the study group, and 40 healthy children delivered during the same period in our hospital were selected as the control group. The distribution of rs3999950 locus genes in the two groups was analyzed. Results: The 260/280 values of the two groups were about 1.8 when the blood genomic DNA concentration was between 226 and 360ng/ml, which indicated that the extracted genomic DNA had good quality and could be used in PCR amplification. The size of the 5th exon of TBX_20 was 350 bp, which was the same as the TBX_20 gene corresponding to the designed primers. There was no non-specific band. The TBX_20 gene fragments obtained after digestion of restriction enzymes by XbaI and EcoRI are similar to theoretical predictions. The success rate of DNA sample acquisition was more than 99% in both groups. Comparing the sequence and sequencing results of TBX_20 gene obtained from NCBI database, it was found that both of them had the same PCR product, and the sequence homology reached 99.4%. The results showed that the distribution of TBX_20 SNP was consistent with Hardy-Weinberg genetic equilibrium. The SNP locus of TBX_20 gene was rs3999950:c.774T＞C (Ala265Ala). Hardy-Weinberg genetic balance test was carried out to evaluate the population representativeness. The distribution of TBX_20 SNP was consistent with Hardy-Weinberg genetic balance. The frequency of TC genotype at rs3999950 locus in the study group was significantly higher than that in the control group (P＜0.05), The difference of C gene locus between the two groups was also statistically significant (P＜0.05). TC genotype OR＞1 increased the incidence of congenital heart disease. Conclusion: The SNP of TBX (20) gene rs3999950 may be closely related to the occurrence of neonatal congenital heart disease, which deserves clinical attention. 2020 Vol. 26 (4): 660-664 [Abstract] ( 110 ) HTML (1 KB)  PDF (1212 KB)  ( 335 ) 664 Clinical Effect of Thoracoscopic Internal Fixation in Treatment Multiple Rib Fractures and Analysis of Analysis the Ameliorative Effect of Pulmonary Ventilation Disorde LUO Hua, PENG Jianming, YUAN Yuexi DOI: 10.3969/j.issn.1006-6233.2020.04.032 Objective: To analyze the thoracoscopic internal fixation in treatment multiple rib fractures and analysis of analysis the ameliorative effect of pulmonary ventilation disorder.Methods: 112 patients with multiple rib fractures who were treated in our hospital from October 2017 to January 2019, theoe patients were divided into control group (n=54) and treatment group (n=58) according to simple random method. The control group was treated with traditional open reduction and internal fixation, while the treatment group was treated with thoracoscopic internal fixation. The operation conditions, clinical efficacy, visual analogue scale (VAS), blood gas analysis, changes in pulmonary ventilation function before and after the operation and complications between the two groups were compared. Results: The incision length, operation time, intraoperative blood loss, postoperative thoracic drainage volume, postoperative ventilator usage time and hospitalization time of the treatment group were lesser than those in the control group (P<0.05). On the 1st and 3rd day after operation, VAS and arterial blood carbon dioxide partial pressure(PaCO2) in both groups decreased. The treatment group was lower than the control group, while arterial oxygen partial pressure(PaO2) in both groups increased. The treatment group was higher than the control group. 14 days and 6 months after operation, the forced expiratory volume (FEV1), maximum ventilation volume (MVV) and forced vital capacity (FVC) in the first second of the two groups all increased. the treatment group was higher than the control group. VAS, blood gas analysis and pulmonary ventilation function indexes of the two groups had interaction between time and groups. there were statistically significant differences between the two groups in the above indicators 1d and 3d postoperatively (P<0.05), there were statistically significant differences within two groups in the above indicators 1d and 3d postoperatively(P<0.05). The incidence of complications in the treatment group was lower than that in the control group (P<0.05).Conclusion: Thoracoscopic internal fixation is safe and effective in the treatment of multiple rib fractures, which can effectively reduce pulmonary ventilation dysfunction with less postoperative complications. 2020 Vol. 26 (4): 664-668 [Abstract] ( 101 ) HTML (1 KB)  PDF (1199 KB)  ( 172 ) 669 Effects of Notch Signaling Pathway on Proliferation Invasion and Migration of Prostate Cancer PC-3 cells LI Chuangui, WANG Qiang DOI: 10.3969/j.issn.1006-6233.2020.04.033 Objective: To investigate the effects and the mechanism of DAPT blocking and NICD adenovirus activating Notch signaling pathway on proliferation, invasion and migration of prostate cancer PC-3 cells and its possible mechanism. Methods: Efficiency of DAPT blocking and AdNICD activating the Notch signaling pathway was detected by real-time PCR. EdU assay was used to evaluate the cell proliferation. Transwell assay and wound healing assay were used to detect the invasion and migration ability of PC-3 cells, respectively. p-AKT protein expression was detected by using western blot assay. Results: DAPT effectively blocked Notch signaling pathway. After adding DAPT to prostate cancer PC-3 cells, the proliferation, invasion and migration ability of cells decreased significantly, and the phosphorylation level of AKT reduced obviously. Infection of NICD adenovirus effectively activated Notch signaling pathway. After adding AdNICD to prostate cancer PC-3 cells, the proliferation, invasion and migration ability to cells increased significantly, and the phosphorylation level of AKT protein raised evidently. The difference was statistically significant. Conclusion: Notch signaling pathway can affect the proliferation, invasion and migration of PC-3 cells by regulating Akt signaling pathway. 2020 Vol. 26 (4): 669-673 [Abstract] ( 148 ) HTML (1 KB)  PDF (2041 KB)  ( 268 ) 673 Analysis of Organ Function Damage and Prognostic Factors in Children with Different Types of Respiratory Tract Infection LU Rong, LIU Qing, PANG Suijun DOI: 10.3969/j.issn.1006-6233.2020.04.034 Objectives: To analyze and discuss the organ function damage and the influencing factors of prognosis in children with different types of respiratory tract infection. Methods: The clinical data of 74 children with respiratory tract infection treated in our hospital from January 2016 to December 2016 were retrospectively analyzed.According to the different types of infection, the children were divided into the upper respiratory tract infection (URTI) group and the pneumonia group, and the clinical data and biochemical indexes of the two groups were collected, and the functional damage of the organs of the children was checked by using the blood cell analysis and the chest positive slice.After follow-up until December 31, 2018, the prognosis of children in each group was observed. The risk factors affecting respiratory tract infection were analyzed by univariate and multivariate Logistic. Results: 5 (14.28%) of the patients with impaired cardiovascular system in the URTI group were significantly lower than that of the pneumonia group (48.72%), and the difference was statistically significant (χ2=9.98, P =0.02); There were 6 cases (17.14%) with abnormal digestive system in URTI group and 21 cases (53.85%) with abnormal digestive system in pneumonia group, the difference was statistically significant (χ2=10.723, P=0.001). As of December 31, 2018, 35 cases (100.00%) were cured in URTI group and 32 cases (82.05%) were cured or improved in pneumonia group. The results of factor analysis showed that there were significant differences in age, asthma, abnormal ALT, abnormal digestive system, the number of severe pneumonia and the level of PCT between the children with pneumonia and those with good prognos is (P<0.05). Multivariate analysis showed that CK-MB was abnormal, and the PCT was an independent risk factor that affected the prognosis of children with pneumonia (P<0.05). Conclusion: Compared with the URTI group, the organ function of the children with pneumonia is more serious; and the CK-MB>24U/L and PCT are the risk factors that affect the prognosis of the children with pneumonia, and the clinical can reduce the incidence of respiratory tract infection by controlling the above factors. 2020 Vol. 26 (4): 673-677 [Abstract] ( 102 ) HTML (1 KB)  PDF (1200 KB)  ( 191 ) 677 Analysis of the Analgesic Effect of Intermittent Epidural Administration on Primipara and its Influence on NSE S100 β and a β LIU Yumei, WANG Zhanhui, ZHANG Xiuxin, et al DOI: 10.3969/j.issn.1006-6233.2020.04.035 Objective: To study the effect of intermittent epidural administration on labor analgesia and the effects of neuron specific enolase (NSE), S100β protein (S100β) and amyloid β protein (Aβ) in primipara. Methods: 86 primiparas received labor analgesia from January 2018 to March 2019 in our hospital were selected into the study object. They were divided into observation group and control group by simple random grouping method, 43 cases in each group. Two groups of patients were given epidural labor analgesia, the observation group of epidural regular intermittent administration, the control group of epidural continuous administration. The changes of vas, NSE, S100 β and a β levels before and after analgesia were compared between the two groups. In addition, the delivery mode and adverse reactions during analgesia were compared between the two groups. Results: There was no significant difference in VAS score between the two groups at different time points before and after analgesia (P>0.05).The VAS score of the two groups at different time points after analgesia was significantly lower than that before analgesia (P<0.05).The natural delivery rate in the observation group was significantly higher than that in the control group, and the rates of vaginal midwifery and cesarean section were significantly lower than those in the control group (P<0.05).There was no significant difference in NSE, S100β and Aβ levels between the two groups before and after analgesia (P>0.05).There was no significant difference in the incidence of adverse reactions between the two groups (9.30% vs 16.28%) during analgesia (P>0.05). Conclusion: Both regular intermittent epidural administration and continuous epidural administration can be used for labor analgesia of primipara with satisfactory analgesic effect and low incidence of adverse drug reactions. They can maintain the balance of NSE, S100β and Aβ. In contrast, regular intermittent epidural administration has the advantage of high natural delivery rate and has high clinical application value. 2020 Vol. 26 (4): 677-681 [Abstract] ( 88 ) HTML (1 KB)  PDF (1199 KB)  ( 314 ) 681 Analysis of Efficacy and Safety of Microscopic Minimally Invasive Surgery and Traditional Craniotomy Surgery for Parasagittal Sinus and Parafalx Cerebri Meningiomas HUANG Bin, TAN Zhiling, WANG Rongyan, et al DOI: 10.3969/j.issn.1006-6233.2020.04.036 Objective: To compare the efficacy and safety of microsurgery with traditional craniotomy in the treatment of parasagittal sinus meningiomas and parafalx meningiomas. Methods: From January 2015 to January 2018, 86 patients with parasagittal sinus meningiomas and parafalx meningiomas in our hospital were selected for the study. They were divided into observation group (46 cases) and control group (40 cases) by simple random number table method. Observation group was treated with microsurgery and control group with traditional craniotomy. The perioperative condition, Simpson grade of tumor resection, symptom relief and recurrence rate were compared between the two groups. Results: The operation time in the observation group was significantly longer than those in the control group, the amount of bleeding and blood transfusion during operation were significantly less than those in the control group, and the hospitalization time was significantly shorter than those in the control group(P＜0.05). The Simpson grading of tumor resection between the two groups has statistically significant (P＜0.05). After operation, the symptoms of limb dysfunction, sensory disturbance and epilepsy in the observation group were significantly improved compared with those before operation, while the limb dysfunction in the control group were significantly improved (P＜0.05). The limb dysfunction in the observation group was significantly lower than those in the control group (P＜0.05). In the follow-up results showed that the total recurrence rate in the observation group was significantly lower than those in the control group (P＜0.05). Conclusion: Compared with traditional craniotomy surgery, the microsurgical minimally invasive surgery is more effective for parasagittal sinus and parafalx cerebri meningiomas, it has the advantages of less trauma, promoting the recovery of clinical symptoms and low recurrence rate., which is worthy of application and promotion. 2020 Vol. 26 (4): 681-686 [Abstract] ( 109 ) HTML (1 KB)  PDF (1212 KB)  ( 436 ) 686 Clinical Study of Simple Four-point Traction Method and Purse-string Suture Traction Method in the treatment of Severe Rickets with Anastomotic Sacral Hemorrhoids LI Guoli, ZHANG Suyun DOI: 10.3969/j.issn.1006-6233.2020.04.037 Objective: To explore the clinical effect of simple four-point traction method and purse-string suture traction method on the treatment of severe rickets with anastomotic sacral hemorrhoidectomy (PPH). Methods: 60 patients with severe rickets admitted to our hospital from March 2018 to May 2019 were enrolled. The patients were divided into study group and control group according to the simple randomization. The study group adopted a simple four-point traction method. The control group was subjected to a purse-string suture traction method. Visual analog scale (VAS) was used to assess the degree of pain in patients. Defecation incontinence scale (FISI) and defecation obstruction (ODS) scores were used to estimate the degree of defecation incontinence and obstruction. The VAS scores and surgical related indexes were compared between the two groups. FISI score, ODS score, clinical efficacy, postoperative complications. Results: The VAS score of the study group was significantly lower than that of the control group. The intraoperative blood loss of the study group was significantly higher than that of the control group, and the difference was statistically significant (P<0.05). The operation time and postoperative hospital stay of the two groups were statistically significant (P<0.05). The difference was not statistically significant (P>0.05). The FISI score and ODS score of the two groups were significantly lower than those before operation (P<0.05). The FISI score and ODS score of the study group were significantly lower than those of the control group. The total effective rate of the study group was 96.7%, which was higher than that of the control group, but the difference was not statistically significant (P>0.05). The postoperative complication rate of the study group was 6.7%, which was lower than that of the control group (20.0%), but the difference was not statistically significant (P>0.05). Conclusion: PPH treatment of severe rickets using simple four-point traction method is more effective than the purse-string suture traction method in reducing pain, defecation incontinence, obstruction, and reducing postoperative complications. It is worth promoting. 2020 Vol. 26 (4): 686-689 [Abstract] ( 113 ) HTML (1 KB)  PDF (1186 KB)  ( 239 ) 690 Analysis on Correlation between Inflammatory Cytokines in Type 2 Diabetes Mellitus and Cardiovascular and Cerebrovascular Diseases SHAN Hong, WU Yanxiang DOI: 10.3969/j.issn.1006-6233.2020.04.038 Objective: To investigate the correlation between inflammatory cytokines in type 2 diabetes and cardiovascular and cerebrovascular diseases. Methods: From January 2018 to April 2019, 114 patients with type 2 diabetes admitted to our hospital were divided into two groups: the non combined group and the combined group, with 57 cases in each group. In addition, 57 outpatients were selected as the healthy group, All the selected persons were examined by carotid ultrasound to understand the pathological changes, and the levels of TNF - α, C-reactive protein, soluble intercellular adhesion molecule-1, soluble vascular cell adhesion molecule-1 and monocyte chemoattractant protein were detected by blood sampling. The results were analyzed and summarized. Results: The carotid intima thickening, the formation of carotid atherosclerotic plaque and the detection rate of carotid stenosis in the healthy group, the non combined group and the combined group were all gradually increased (P<0.05). The levels of TNF - α, C-reactive protein, soluble intercellular adhesion molecule-1, soluble vascular cell adhesion molecule-1 and monocyte chemoattractant protein in the healthy group, the non combined group and the combined group were gradually increased, and the difference was statistically significant (P<0.05). Conclusion: There is a correlation between the levels of inflammatory factors and cardiovascular and cerebrovascular diseases in type 2 diabetes. Inflammatory factors are risk factors for cardiovascular and cerebrovascular diseases in patients with type 2 diabetes. 2020 Vol. 26 (4): 690-693 [Abstract] ( 94 ) HTML (1 KB)  PDF (1179 KB)  ( 315 ) 693 Analysis on Correlation between the Level of Ficolin-3 in Plasma and the Stage and Degree of Mycoplasma Pneumoniae Infection in Children CAO Yanling, GAO Jiaojiao, LI Wenbin, et al DOI: 10.3969/j.issn.1006-6233.2020.04.039 Objective: To study the correlation between plasma fibrin gel -3 (Ficolin-3) level and disease stages, severity of children with Mycoplasma pneumoniae (MP) infection. Methods: 78 children with MP infection admitted to the Department of Pediatrics from June 2018 to June 2019 were selected as the study objects, and 88 children with MP infection were selected as the control group, the expression levels of ficolin-3 in plasma, interleukin-8 (IL-8), interleukin-10 (IL-10), tumor necrosis factor (TNF-α) were measured by enzyme-linked immunosorbent assay (ELISA), detection of serum immunoglobulin (IgM, IGA) expression by immunoturbidimetry and the correlations between the expression level of ficolin-3 and disease stage, disease degree, IL-8, IL-10, TNF-α, IgM, IgA were analyzed. Results: Compared with the control group, the levels of ficolin-3, IL-8, TNF-α and IgM in the plasma of MMP group were significantly higher (P<0.05), while the levels of IL-10 and IgA were significantly lower (P<0.05). Compared with the convalescent group, the levels of ficolin-3, IL-8, TNF-α and IgM in the plasma of the acute group were significantly higher (P<0.05), while the levels of IL-10 and IgA decreased significantly (P<0.05). Compared with the mild group, the expression levels of ficolin-3, IL-8, TNF-α and IgM in the severe group were significantly higher (P<0.05), while the expression levels of IL-10 and IgA were significantly lower (P<0.05). The expression level of ficolin-3 in plasma of children with MMP was positively correlated with disease stage, disease severity, the expression levels of IL-8, TNF-α and IgM in serum (P<0.05), and negatively correlated with the expression levels of IL-10 and IgA in serum (P<0.05). Conclusion: The expression level of ficolin-3 in plasma of MMP patients is significantly increased, which is positively correlated with disease stage, disease degree, expression levels of serum IL-8, TNF-α and IgM, and negatively correlated with the expressions of serum IL-10 and IgA, it may be a clinical index to evaluate the condition of children with Mycoplasma pneumoniae pneumonia (MMP). 2020 Vol. 26 (4): 693-697 [Abstract] ( 92 ) HTML (1 KB)  PDF (1209 KB)  ( 362 ) 698 The Repairing Effect of CGF Combined with Fibroin Hydroxyapatite Scaffold on Rabbit Mandible Defect CHEN Qi, LI Shiyan, YU Xin DOI: 10.3969/j.issn.1006-6233.2020.04.040 Objective: To explore the role of concentrated growth factor (CGF) complex silk fibroin-hydroxyapatite scaffold in promoting the repair of rabbit mandibular defects. Methods: 48 healthy New Zealand white rabbits were randomly divided into 3 groups, 16 in each group. The right mandibular bone defect model was prepared at the same time. Group A was a composite material group, and group B was a hydroxyapatite group implanted with hydroxyapatite materialGroup C No material is implanted for the blank group. All animals were harvested at 2, 4, 8, and 12 weeks, and tissue samples were prepared. The results were observed in general observation, imaging, and histological analysis. The imaging analysis values were collected and evaluated. The analysis results were analyzed by SPSS17.0. P<0.05 was statistically significant. Results: Gross observation and imaging analysis showed that the new bone condition and osteogenesis rate of group A were significantly better than those of the other two groups. The bone mineral density analysis and new bone area measurement also showed the superiority of composite materials in promoting osteogenesis. Conclusion: Concentrated growth factor (CGF) combined with silk fibroin hydroxyapatite scaffolds showed a good osteogenic effect in the experiment of promoting jaw defects, with obvious osteoinductive effect, which is expected to become a new material for clinical bone defect repair. 2020 Vol. 26 (4): 698-702 [Abstract] ( 78 ) HTML (1 KB)  PDF (1559 KB)  ( 321 )

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