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2020 Vol. 26, No. 2 Published: 2020-02-29 177 Effect of miR-663 on the Growth Proliferation Migration and Invasion of PG-LH7 and PG-BE1 Cells WANG Yunpeng, et al DOI: 10.3969/j.issn.1006-6233.2020.02.001 Objective: To explore the modulating effect of the microRNA-663 (miR-663) on the proliferation ability and invasive ability in human lung cancer cells PG-LH7 and PG-BE1. Methods: Real time quantitative RT-PCR was used to detect the expression of miR-663 in A549 cells, PG-LH7 cells, PG-BE1 cells, PG-BE1 cells transfected by mir-663ASO and PG-LH7 cells transfected by miR-663mimics; cell count, colony forming assay and CCK8 method were used to detect the proliferation of PG-BE1 cells and PG-LH7 cells transfected by miR-663ASO or miR-663mimics; Transwell cell was used to detect the expression of miR-663ASO or miR-663mimics after transfection The invasion and migration ability of PG-BE1 cells and PG-LH7 cells. Results: The expression level of miR-663 in PG-LH7 cells was lower than that in A549 cells (P<0.05), the expression level of miR-663 in PG-BE1 cells was higher than that in A549 cells (P<0.05), the expression level of miR-663 in PG-LH7 was increased by miR-663mimics transfection (P<0.05), the expression level of miR-663 in PG-BE1 was significantly decreased by miR-663ASO transfection (P<0.05), and the expression level of miR-663mimics was effective MiR-663ASO can effectively inhibit the proliferation and invasion of PG-BE1 cells (P<0.05). Conclusion: Overexpression of miR-663 can promote the proliferation, invasion and migration of PG-LH7, a low metastasis sub line of human lung cancer, while inhibition of miR-663 can inhibit the growth, proliferation, migration and invasion of PG-BE1, a high metastasis sub line. 2020 Vol. 26 (2): 177-180 [Abstract] ( 200 ) HTML (1 KB)  PDF (1412 KB)  ( 305 ) 181 Effects of Low-dose l-t4 on Biochemical Markers of Benign Small Nodules and Its Influencing Factors WANG Shangnong, et al DOI: 10.3969/j.issn.1006-6233.2020.02.002 Objective: To investigate the effect of low-dose l-t4 on the biochemical markers of benign small nodules and the influencing factors. Methods: From January 2016 to January 2018, 78 patients with benign thyroid nodules were enrolled in the endocrinology department of our hospital. They were divided into observation group and control group according to the random number table method. Small doses were taken for the observation group. Left thyroid hormone (l-t4) treatment, the course of treatment is 1 year, no drug treatment for the control group, only clinical observation and follow-up. TSH, JV, JD3, small nodule diameter and thyroid-stimulating hormone levels and serum free thyroxine (FT4) were measured every 3 months. The detection is performed in 4 cycles horizontally. At the same time, a total study of 39 patients in the observation group was conducted to investigate the effect of gender and age on the diameter of small thyroid nodules in patients. Results: After 12 months of treatment, the thyroid volume (8.52±8.68) cm3 and the maximum nodule cross-section diameter (47.88±8.54) mm in the observation group were significantly smaller than those in the control group (13.16±9.88) cm3 and (63.48±10.24) mm, and the difference was statistically significant (P<0.05). After 12 months, the diameter of small nodules in the observation group (4.84±1.80) mm was significantly lower than that in the control group (6.33±1.91) mm, and the difference was statistically significant (P<0.05). After 12 months of treatment, the level of thyrotropic hormone (0.36±0.14) mIU/L in the observation group was significantly lower than that in the control group (1.86±0.78) mIU/L, and serum free thyroxine (26.24±4.04) pmoL/L was significantly higher than that in the control group (16.88±3.52) pmoL/L, the difference was statistically significant (P<0.05). After 12 months, the diameter of small nodules and TSH of the female patients in the observation group were (4.32±1.76) mm and (0.31±0.10) mIU/L, respectively, which were significantly lower than those of the male patients (5.62±1.76) mm and (0.46±0.15) mIU/L, and the difference was statistically significant (P<0.05). FT4 was (29.61±5.42) pmoL/L, which was significantly higher than (21.26±4.37) pmoL/L in men, and the difference was statistically significant (P<0.05). The diameter of small nodules and TSH of patients <45 years old were (4.94±1.26) mm and (0.34±0.13) mIU/L, respectively, and the values of (5.86±1.58) mm and (0.49±0.15) mIU/L of patients ≥45 years old were significantly reduced (P<0.05). FT4 was (28.81±3.07) pmoL/L, which was significantly lower than that of male (22.60±4.00), and the difference was statistically significant (P<0.05). Conclusion: Low-dose l-t4 can effectively control the thyroid benign small nodule biochemical index to inhibit the secretion of TSH and increase the FT4 level and control the growth of benign small nodules. It is more effective for female patients and patients <45 years old. 2020 Vol. 26 (2): 181-184 [Abstract] ( 183 ) HTML (1 KB)  PDF (1251 KB)  ( 437 ) 185 Effect of Astragalus Membranaceus on Viral Myocarditis Inflammatory Factors and Cellular Immunity XU Hui, NIU Ling, AN Xinjiang, et al DOI: 10.3969/j.issn.1006-6233.2020.02.003 Objective: To investigate the effect of Astragalus membranaceus on inflammatory factors and cellular immunity of viral myocarditis patients. Methods: 70 children with viral myocarditis hospitalized in our hospital from April 2016 to March 2018 were selected as the study subjects. According to the random number table method, they were divided into study group (38 cases) and control group (32 cases).The control group received conventional treatment, while the study group continued to use astragalus on the basis of the former.The clinical efficacy, inflammatory factors (TNF-alpha, il-6, il-17) and cellular immunity of the two groups were compared before and after treatment.To evaluate the clinical value of astragalus in children with viral myocarditis. Results: The total effective rate in the study group (89.47%) was greater than that in the control group (68.75%) (χ2 =4.663, P<0.05). Repeated measurement analysis of variance showed that the time point, group, time point and interaction effect of CK, CK-MB, LD, cTnI, TNF - α, IL-6, IL-17, CD3 +, CD4 +, CD4 + / CD8 + levels in the two groups were statistically significant (P<0.05), and the levels of CK, CK-MB, LD, cTnI, TNF - α, IL-6, IL-17 in the study group were lower than those in the control group at 1 or 2 weeks after treatment. The levels of CD3 +, CD4 +, CD4 + / CD8 + were significantly higher than those of the control group (P<0.05). Conclusion: Astragalus membranaceus has a significant effect on children with viral myocarditis. It can effectively improve the immune function of children, reduce the incidence of inflammation, and has a positive significance for the recovery of children after treatment. 2020 Vol. 26 (2): 185-189 [Abstract] ( 160 ) HTML (1 KB)  PDF (1256 KB)  ( 307 ) 190 Efficacy of Sodium Creatine Phosphate in Treatment of Viral Myocarditis in Children and Its Effect on Myocardial Enzyme and Troponin Levels MA Ke, KOU Yan, LU Rong, et al DOI: 10.3969/j.issn.1006-6233.2020.02.004 Objective: To investigate the therapeutic effect of sodium creatine phosphate on viral myocarditis in children and the effect on myocardial enzyme and troponin level. Methods: 94 children with viral myocarditis admitted to our hospital between March 2017 and October 2018 were selected as the subjects of the study. With the consent of the family members of the children, the children were divided into control group and observation group by simple random digital table method. Routine treatment and routine treatment combined with sodium creatine phosphate therapy were performed respectively. The therapeutic effects of the two groups were compared, and the levels of myocardial enzymes and troponin were compared between the two groups. Results: The total effective rate of the observation group was higher than that of the control group (P<0.05), and the CK,CK-MB,LDH,AST level of the two groups decreased after treatment (P1 <0.01, P2 <0.01), and the total effective rate of the observation group was higher than that of the control group (P<0.05). The comparison between the two groups showed that there was no significant difference in myocardial enzyme level between the two groups before treatment (P>0.05). After treatment, the level of CK,CK-MB,LDH,AST in the observation group was lower than that in the control group, the difference was statistically significant (P<0.01), but there was no significant difference in the level of cTnI between the two groups before treatment (P>0.05). After treatment, the level of cTnI in the observation group was significantly lower than that in the control group. Conclusion: In the treatment of viral myocarditis in children, sodium creatine phosphate has obvious therapeutic effect, can effectively reduce the level of myocardial enzymes and troponin, improve the prognosis of children, and is worthy of clinical promotion. 2020 Vol. 26 (2): 190-193 [Abstract] ( 161 ) HTML (1 KB)  PDF (1223 KB)  ( 385 ) 194 Correlation between Serum Galactose Lectin-3 Level and Cardiac Function and Inflammatory Response in Patients with CHF after MI WAN Rong, XU Feng, ZHANG Xiaoxia, et al DOI: 10.3969/j.issn.1006-6233.2020.02.005 Objective: To explore the correlation between serum galactose lectin-3 (Gal-3) levels and cardiac function and inflammatory response in patients with chronic heart failure (CHF) after myocardial infarction (MI). Methods: The clinical data of 156 patients with CHF in our hospital in recent years were analyzed retrospectively. According to the history of MI, 78 patients in the observation group and 78 patients in the control group were divided. The results of Gal-3, inflammation index and echocardiography were compared between the two groups, and the correlation between Gal-3 and cardiac function and inflammation was analyzed. Results: The levels of CRP, TNF - α and Gal-3 in the observation group were (5.93 ± 0.82) mg / L, (16.48 ± 1.05) ng / L, (9.82 ± 1.13) ng / mL, respectively, higher than those in the control group (4.89 ± 0.71) mg / L, (12.17 ± 1.12) ng / L, (6.08 ± 1.04) ng / mL (P<0.05). The LVEF of the observation group was (50.08 ± 2.08)%, lower than that of the control group (57.62 ± 2.19)% (P<0.05). According to Pearson analysis, the level of Gal-3 in the observation group was positively correlated with CRP and TNF - α (r = 0.778, 0.725, P<0.01), and negatively correlated with LVEF (r = -0.854, P<0.01). Conclusion: Gal-3 was positively correlated with inflammatory response and negatively correlated with cardiac function in patients with CHF after MI. 2020 Vol. 26 (2): 194-196 [Abstract] ( 116 ) HTML (1 KB)  PDF (1222 KB)  ( 237 ) 197 Expression of Nuclear Transporter Protein KPNA2 in Colorectal Cancer Tissues and Its Relationship with Clinicopathological Features of Colorectal Cancer MA Chunyan DOI: 10.3969/j.issn.1006-6233.2020.02.006 Objective: To investigate the expression of nuclear transporter protein KPNA2 in colorectal cancer and its relationship with clinicopathological features of colorectal cancer. Methods: 92 patients with colorectal cancer admitted to our hospital from January 2017 to March 2019 were selected as colorectal cancer group. Ninety-two healthy people who had physical examination at the same time were selected as normal control group. Samples of two groups of subjects were collected for immunohistochemical analysis, and the expression of KPNA2 was calculated for all samples. The expression of KPNA2 was compared between the two groups. The difference of KPNA2 expression among patients with different pathological characteristics in colorectal cancer group was compared and logistic regression analysis was performed. Results: The expression rate of KPNA2 in colorectal cancer group was 72.83%, which was higher than that in normal control group. The expression of KPNA2 was different in patients with different tumor size, differentiation degree, T stage, N stage and M stage. Tumor size, T stage, N stage and M stage were positively correlated with KPNA2 expression, while differentiation degree was negatively correlated with KPNA2 expression. All the above were P<0.05. Conclusion: The expression of KPNA2 in colorectal cancer tissues is significantly up-regulated. The high expression of KPNA2 is closely related to the size, differentiation and staging of colorectal cancer. It has certain diagnostic value in judging the occurrence and development of colorectal cancer. 2020 Vol. 26 (2): 197-201 [Abstract] ( 99 ) HTML (1 KB)  PDF (1239 KB)  ( 342 ) 202 Researchon Mechanisms of Dexmedetomidine Alleviating Early Brain Injury in Rats with Subarachnoid Hemorrhage by Down-regulating the Expression of NLRP3 Inflammatory Bodies PENG Chengxu, XIANG Hong, JIN Tao, et al DOI: 10.3969/j.issn.1006-6233.2020.02.007 Objective: To investigate the mechanism of dexmedetomidine in reducing early brain injury in rats with subarachnoid hemorrhage by down-regulating the expression of NLRP3 inflammatory body. Methods: 100 healthy SD rats were randomly divided into healthy control group, model group and dexmedetomidine group (low dose group, middle dose group and high dose group), 20 rats in each group. Rats in model group and dexmedetomidine group were used to construct subarachnoid hemorrhage model by internal carotid artery puncture. After the model was established, surviving rats were identified as successful subarachnoid hemorrhage model by Garcia method. After the completion of the model, rats in the model group were injected with normal saline and rats in the dexmedetomidine group were injected with high, medium and low doses of dexmedetomidine respectively. The rats were sacrificed after 7 days of continuous administration. The amount of subarachnoid hemorrhage and water content in the brain were measured and compared. The levels of serum inflammatory factors were detected by ELISA, and the levels of NLRP3 were detected by immunohistochemical method. Pearson correlation test was used to analyze the correlation between inflammatory factors such as interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and NLRP3 levels. Results: Compared with healthy control group, the amount of subarachnoid hemorrhage and water content in brain of model group and dexmedetomidine group increased significantly, and the amount of subarachnoid hemorrhage and water content in brain of dexmedetomidine group decreased. The amount of hemorrhage and water content of dexmedetomidine group decreased with the increase of dosage. There was significant difference between the two groups (P<0.05). Compared with the healthy control group, the levels of IL-1beta, TNF-alpha, IL-6 and NLRP3 in the model group and dexmedetomidine group were significantly higher, and the levels of inflammatory factors in dexmedetomidine group were lower than those in the model group. The levels of inflammatory factors in dexmedetomidine group decreased gradually with the increase of dosage, and there were significant differences among the groups (P<0.05). Pearson correlation test showed that NLRP3 was positively correlated with IL-1beta, TNF-alpha and IL-6 (P<0.05). Conclusion: dexmedetomidine can reduce the degree of early brain injury, inflammatory response and NLRP3 level in rats with subarachnoid hemorrhage, and the mechanism may be related to down-regulation of the expression of NLRP3 inflammatory bodies. 2020 Vol. 26 (2): 202-204 [Abstract] ( 140 ) HTML (1 KB)  PDF  (0 KB)  ( 124 ) 205 Logistic Regression Analysis of Factors Related to Coronary Stent Restenosis after Stent Implantation in Patients with Coronary Atherosclerotic Heart Disease LU Xiaocao, WANG Xiaolin DOI: 10.3969/j.issn.1006-6233.2020.02.008 Objective: To investigate the influence of Logistic regression analysis on the related factors of in-stent restenosis (ISR) in patients with coronary atherosclerotic heart disease (CHD) after stent implantation. Methods: The clinical data of 105 patients with CHD who underwent stent implantation were retrospectively analyzed. According to the occurrence of ISR after stent implantation, the related factors affecting the occurrence of ISR after stent implantation were analyzed by single factor and multi-factor logistic regression. Results: Among 105 patients with CHD, there were 19 cases of ISR with the incidence 18.10% after operation. Univariate analysis showed that the incidence of ISR in patients with family history of CHD, diabetes mellitushistory, hypertensionhistory, smoking history, stent ≥ 3 pieces, stent length ≥ 30 mm, stent diameter < 3.00 mm, tandem stent, low-density lipoprotein-cholesterol (LDL-C) ≥ 3.37 mmoL/L, routine dosage of statins and discontinuation of aspirin were higher than those without family history of CHD, no diabetes mellitus history, nohypertensionhistory, nosmokinghistory, stent < 3 pieces, stent length < 30 mm, stent diameter < 3.00 mm, noseries stent, LDL-C < 3.37 mmoL/L, high dose of statins, no discontinuation of aspirin (P<0.05). Multivariate logistic regression analysis showed that family history of CHD, history of diabetes mellitus, history of hypertension, smoking history, stent ≥ 3 pieces, stent length ≥ 30 mm, stent diameter < 3.00 mm, tandem stent, LDL-C ≥ 3.37 mmoL/L, routine dosage of statins and discontinuation of aspirin were independent risk factors for coronary ISR in patients with CHD after stent implantation (P<0.05). Conclusion: There are many factors influencing the occurrence of ISR in patients with CHD after stent implantation. Therefore, it is necessary to evaluate the factors reasonably in clinic, and to improve the prognosis of patients with possible ISR in time and with emphasis on the treatment of patients with underlying diseases and the results of coronary angiography. 2020 Vol. 26 (2): 205-210 [Abstract] ( 119 ) HTML (1 KB)  PDF (1250 KB)  ( 351 ) 211 Efficacy of Tripterygium Wilfordii Polyglycoside Combined with Taprolimus in the Treatment of Idiopathic Membranous Nephropathy and its Effect on Urinary C5b-9 and IgG4 LIANG Yan, YAN Xiaohui, WANG Xiaoming, et al DOI: 10.3969/j.issn.1006-6233.2020.02.009 Objective: To study the efficacy of tripterygium wilfordii polyglycoside combined with taprolimus in the treatment of idiopathic membranous nephropathy and its effect on urinary Urinary membrane attack complex (c5b-9), immunoglobulin G4 (IgG4). Methods: From Februny 2015 to January 2016, 92 patients with idiopathic membranous nephropathy were divided into observation group and control group according to the order of admission. The control group was treated with tacrolimus on the basis of conventional therapy. The observation group was treated with tripterygium glycosides on the basis of the control group. The clinical efficacy, renal function, urinary C5b-9, IgG4 and sex hormone levels were compared between the two groups. Results: After treatment, the total effective rate of the observation group was significantly higher than that of the control group [93.48% (43/46) vs 71.27% (33/46)] (P<0.05). The level of serum albumin in the observation group was significantly higher than that in the control group [(34.21 ± 3.87) g vs (24.25 ± 2.42) g] (P<0.05), and the 24h urine protein level was significantly lower than that in the control group [(1.15 ± 0.17) g vs (2.34 ± 0.21) g] (P<0.05). The levels of urinary C5b and IgG4 in the observation group were significantly lower than those in the control group [(45.98 ± 4.32) ng / mg, (65.21 ± 6.54) ug / mmoL vs(1.46 ± 0.21) ng / mg, (6.32 ± 0.69) ug / mmoL] (P<0.05). The levels of SBG, E2 and T in the observation group were significantly higher than those in the control group [(37.54 ± 3.14 nmoL /L, (42.16 ± 4.45) pg /mL, (350.54 ± 35.98) ng/dL vs (26.98 ± 2.87) nmoL / L (35.87 ± 3.76) pg/mL, (287.43 ± 23.76) ng/dL] (P<0.05), FSH level was significantly lower than that of the control group [(6.12 ± 0.65) mIU / mL vs (7.48 ± 0.79) mIU / mL] (P<0.05). Conclusion: Tacrolimus combined with tripterygium wilfordii polyglycoside can be used to treat patients with idiopathic membranous nephropathy, which can effectively reduce the levels of urinary C5b-9 and IgG4, and have little effect on sex hormone levels and good clinical curative effect. 2020 Vol. 26 (2): 211-214 [Abstract] ( 180 ) HTML (1 KB)  PDF (1235 KB)  ( 416 ) 215 Effects of Low and High Doses of Caffeine Citrate on the Numberof Apnea Related Adverse Events and Clinical Outcomes in Premature Infants with Primary Apnea LIU Zhihua, SHI Lili, et al DOI: 10.3969/j.issn.1006-6233.2020.02.010 Objective: To investigate the effects of low-dose and high-dose caffeine citrate on apnea frequency, related adverse reactions and clinical outcomes in premature infants with primary apnea. Methods: 128 children with primary apnea admitted to our hospital from January 2017 to December 2018 were randomly divided into two groups, 64 in each group. The control group was treated with low-dose caffeine citrate, while the observation group was treated with high-dose caffeine citrate. The therapeutic effects, adverse events and clinical outcomes of the two groups were compared. Result: The number of apnea in the observation group was significantly less than that in the control group, and the success rate of weaning and the effective rate of treatment were significantly higher than those in the control group, the difference was statistically significant (all P<0.05). There was no significant difference in the days of oxygen therapy and mechanical ventilation between the two groups (all P>0.05). There was no significant difference in adverse reaction events between the two groups (P>0.05). There was no significant difference in the days of oxygen therapy and mechanical ventilation between the two groups (all P>0.05). There was no significant difference in adverse reaction events between the two groups (P>0.05). There was no significant difference in clinical outcomes between the two groups (P>0.05). Conclusion: When the initial dose of caffeine citrate is 20 ms/kg and the daily maintenance dose is 15 ms/kg, the therapeutic effect of caffeine citrate on primary apnea of premature infants is remarkable. 2020 Vol. 26 (2): 215-217 [Abstract] ( 95 ) HTML (1 KB)  PDF (1222 KB)  ( 345 ) 218 Diagnosis the Value of Dynamic Contrast-enhanced MRI Combined with Serum CA15-3 and CEA on Breast Cancer LI Yuemei, ZHONG Weixiu, YANG Liping DOI: 10.3969/j.issn.1006-6233.2020.02.011 Objective: To explore the diagnostic value of dynamic contrast-enhanced magnetic resonance imaging (MRI) combined with serum CA15-3 and carcinoembryonic antigen (CEA) for breast cancer. Methods: 80 patients with breast cancer admitted to the hospital from January 2018 to February 2019 were included in the breast cancer group. 50 patients with benign breast tumors were selected and included in the benign group, respectively. All patients underwent dynamic contrast-enhanced MRI. Serum CA15-3 and CEA levels were detected. The differences in serological indicators among groups and the value of the indicators alone or combined diagnosis for breast cancer were analyzed. Results: MRI findings of malignant breast lesions showed unclear boundaries with surrounding tissues, irregular margins or burrs, and inhomogeneous enhancement. Findings of benign breast lesions showed masses with clear boundaries with surrounding tissues and smooth edge, round or lobulated shape, and homogeneous enhancement of most lesions. Levels of CA15-3 and CEA in the breast cancer group were significantly higher than those in the benign group (P<0.05). For alone diagnosis, the diagnostic sensitivity and accuracy of CEA were the lowest while diagnostic sensitivity and accuracy of MRI were the highest. The diagnostic sensitivity of MRI combined with CA15-3 and CEA was significantly higher than that of MRI, serum CA15-3 or CEA alone (P<0.05), while the specificity was lower than the other three methods. Conclusion: Dynamic contrast-enhanced MRI combined with serum CA15-3 and CEA can improve the diagnostic sensitivity of breast cancer, which is of value for the early diagnosis of breast cancer. 2020 Vol. 26 (2): 218-221 [Abstract] ( 109 ) HTML (1 KB)  PDF (1703 KB)  ( 311 ) 222 Effects of Scalp-needle Interaction MOTOmed Intelligent Exercise Training on Limb Motor Function and Levels of Serum NGF and BDNF in Patients with Stroke Hemiplegia LI Yingying, FU Qiao, WU Weiwei, et al DOI: 10.3969/j.issn.1006-6233.2020.02.012 Objective: To investigate the effects of scalp-needle interaction MOTOmed intelligent exercise training on limb motor function and levels of serum nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) in patients with stroke hemiplegia. Methods: 80 patients with stroke hemiplegia were divided into control group and study group according to the simple random grouping of random number table method, with 40 cases in each group. The patients were treated with conventional drug symptomatic support, and control group was given routine rehabilitation training, and study group was given scalp-needle interaction MOTOmed intelligent exercise training. The clinical efficacy, limb motor function, balance function, daily activity ability, neurotrophic factor (NGF,BDNF) and quality of life level were compared between the two groups. Results: After treatment, The total effective rate of treatment in study group was significantly higher than that in control group (87.50% vs 65.00%) (P<0.05). After treatment, the upper limb score and lower limb score of Fugl-Meyer motor function assessment scale (FMA), Berg balance scale (BBS) score and modified Barthel index (MBI) score in the two groups were significantly improved compared with those before treatment (P<0.05), and the differences of FMA-upper limb score, FMA-lower limb score, BBS score and MBI score before and after treatment in study group were significantly higher than those in control group (P<0.05). The levels of NGF, BDNF and neurotrophin-3 (NT-3) in the two groups were significantly higher than those before intervention (P<0.05), and the differences of GF, BDNF and NT-3 before and after treatment in study group were significantly higher than those in control group (P<0.05). After treatment, the Stroke Speciality-Quality of Life (SS-QOL) scores in the two groups were significantly increased compared with those before treatment (P<0.05), and the difference of SS-QOL score before and after treatment in study group were significantly higher than those in control group (P<0.05). Conclusion: The rehabilitation treatment regimen of scalp-needle interaction MOTOmed intelligent exercise training has exact efficacy in treating patients with stroke hemiplegia. It can improve the limb motor function, balance function and daily activity function, enhance the levels of neurotrophic factors and promote the recovery of damaged nerves, thus improving the quality of life of patients. 2020 Vol. 26 (2): 222-225 [Abstract] ( 84 ) HTML (1 KB)  PDF (1242 KB)  ( 334 ) 226 Effects of Beraprost Sodium on Scr BUN FIB D-D TNF-alpha, hs-CRP in Elderly Patients with Glomerulosclerosis of T2DM LI Hongwei, WANG Zhiqi, ZHANG Ju DOI: 10.3969/j.issn.1006-6233.2020.02.013 Objective: To investigate the effects of beraprost sodium on serum inflammatory factors, coagulation function and renal function in elderly patients with type 2 diabetes mellitus (T2DM) with glomerulosclerosis. Methods: 78 elderly patients with T2DM glomerulosclerosis admitted to our hospital from January 2016 to January 2019 were randomly divided into control group (treated with benazepril) and research group (treated with benazepril and beraprost sodium), 39 cases in each group by convenient sampling. The levels of serum inflammatory factor hypersensitive C-reactive protein (hs-CRP), tumor necrosis factor-α (TNF-α), coagulation function index D-dimer (D-D), fibrinogen (Fib) and renal function index blood urea nitrogen (BUN) and serum creatinine (SCr) were compared between the two groups before and after treatment. Results: Compared with before treatment, the contents of hs-CRP and TNF-α in the two groups decreased significantly 2 months after treatment (P<0.05); Compared with the control group, the contents of hs-CRP and TNF-α in the study group decreased significantly 2 months after treatment (P<0.05). The concentration of D-D and Fib in the control group did not change significantly after 2 months of treatment compared with that before treatment (P>0.05); The concentration of D-D and Fib in the study group decreased significantly after 2 months of treatment compared with that before treatment (P<0.05), and decreased significantly after treatment compared with that in the control group (P<0.05). The levels of BUN and SCr in the two groups were significantly lower than those before treatment, and the levels of BUN and SCr in the study group were significantly lower than those in the control group after treatment (P<0.05). Conclusion: Beraprost sodium therapy can effectively improve the coagulation function and renal function of elderly patients with glomerulosclerosis of type 2 diabetes mellitus on the basis of routine treatment, and regulate the level of inflammatory factors in vivo. It has significant therapeutic effect, so it is worthy of clinical application. 2020 Vol. 26 (2): 226-229 [Abstract] ( 121 ) HTML (1 KB)  PDF (1247 KB)  ( 349 ) 230 Curative Effect of Pefloxacin Combined with Minocycline Hydrochloride on Periodontitis and the Influences on Early Periodontal Indexes and Osteocalcin Level in Gingival Crevicular Fluid JIANG Tianjiao, WANG Xin, MA Nan DOI: 10.3969/j.issn.1006-6233.2020.02.014 Objective: To explore curative effect of pefloxacin combined with minocycline hydrochloride on periodontitis patients. Methods: During the period from July 2016 to July 2019, 90 periodontitis admitted to the hospital were enrolled as objects. They were divided into single medication group and combination medication group according to random number table method, 45 cases in each group. The two groups were treated with minocycline hydrochlorid or pefloxacin combined with minocycline hydrochloride, respectively. The curative effect, changes in early periodontal indexes before and after treatment and level of gingival crevicular fluid (GCF) related indexes, incidence of adverse reactions and recurrence rate were compared between the two groups. Results: The total response rate of treatment in combination medication group was higher than that in single medication group (93.33% vs75.56%, P<0.05). After treatment, depth of periodontal pocket, plaque index, clinical attachment loss and gingival index in both groups were significantly improved (P<0.05). The above early periodontal indexes in combination medication group were significantly lower than those in single medication group (P<0.05). After treatment, GCF related indexes such as IL-1β, sICAM-1, osteocalcin and MCP-1 in both groups were decreased (P<0.05). The all GCF indexes in combination medication group were significantly lower than those in single medication group (P<0.05. The incidence of adverse reactions and recurrence rate in combination medication group were lower than those in single medication group. There was no significant difference in incidence of adverse reactions between the two groups (17.78% vs28.89%, P>0.05), while there were significant differences in recurrence rate (2.22% vs20.00%, P<0.05). Conclusion: The curative effect of pefloxacin combined with minocycline hydrochloride is significant on periodontitis, which can significantly improve levels of early periodontal indexes and osteocalcin in GCF. And safety is good. 2020 Vol. 26 (2): 230-234 [Abstract] ( 110 ) HTML (1 KB)  PDF (1237 KB)  ( 353 ) 235 Role of Connective Tissue Growth Factor PD98059 and LY294002 in Regulating the Proliferation and Migration of Pulmonary Artery Smooth Muscle Cells of Rat HU Yu, ZHOU Mengjun, et al DOI: 10.3969/j.issn.1006-6233.2020.02.015 Objective: To explored the proliferation and migration of (PASMC) in rat pulmonary artery smooth muscle cells under the intervention of connective tissue growth factor (CTGF), PD98059 and LY294002. Method: PASMC was isolated and cultured, and then were divided into blank group, CTGF group, CP Group, CL group and CPL group. The effects of CTGF, PD98059 and LY294002 on cell proliferation were detected by WST-1, and the effects on cell migration were detected by Transwell, cell scratch and cytoskeleton. Results: Compared with the blank group, the proliferation level of WST-1 cells increased after CTGF (50NG/ml) stimulation of pasmc48, 72, 96 and 120 hours, the number of transmembrane cells increased after 6h and 12h, the scratch width of cells decreased after 12h and 24h, and the cytoskeleton was fuzzy, the difference was statistically significant (P<0.05). After PD98059 (20 μ mol / L) and / or LY294002 (10 μ moL/L) intervention for 24, 48, 72, 96 and 120 hours, WST-1 showed a decrease in cell proliferation; after 6 and 12 hours, Transwell showed a decrease in the number of transmembrane cells, and after 12 and 24 hours, the scratch width and cytoskeleton of the cells were increased, the difference was statistically significant (P<0.05). Conclusion: CTGF can stimulate the proliferation and migration of PASMC and may be one of the factors to promote the pulmonary vascular remodeling of pulmonary hypertension (PAH). PASMC proliferation and migration can be inhibited by PD98059 and / or LY294002, suggesting that ERK1 / 2 and PI3K pathways blocked by CTGF may participate in the pulmonary vascular remodeling of PAH stimulated by CTGF. 2020 Vol. 26 (2): 235-239 [Abstract] ( 101 ) HTML (1 KB)  PDF (2669 KB)  ( 298 ) 240 Evaluation Value of sCD40L and Lp-PLA2 in Myocardial Ischemia/reperfusion Injury During PCI HAN Tingting, WANG Hong, WU Meimei, et al DOI: 10.3969/j.issn.1006-6233.2020.02.016 Objective: To explore the predictive value of soluble CD40 ligand (sCD40L) and lipoprotein associated phospholipase A2 (Lp-PLA2) in acute myocardial infarction (AMI) patients after emergency intervention. Methods: 100 patients with acute myocardial infarction and PCI were selected for the first time in a row. The ischemia-reperfusion injury, clinical data and laboratory indexes were collected. The concentrations of Lp-PLA2 and sCD40L were detected by enzyme-linked immunosorbent assay (ELISA). The predictive value of Lp-PLA2 and sCD40L on myocardial ischemia-reperfusion injury was analyzed. Results: The levels of Lp-PLA2 and sCD40L in the myocardial ischemia / reperfusion injury group were significantly higher than those in the non myocardial ischemia / reperfusion injury group (P<0.05). Multiple logistic regression analysis showed that Lp-PLA2 and sCD40L were independent risk factors of myocardial ischemia-reperfusion injury (or: 1.655, 1.146, P<0.05). The area under ROC curve of them was 0.740 and 0.697 respectively, and the area under ROC curve was 0.811. Conclusion: LPA 2 and soluble CD40 ligands have a good predictive value for the risk of myocardial reperfusion injury after PCI. 2020 Vol. 26 (2): 240-243 [Abstract] ( 115 ) HTML (1 KB)  PDF (1324 KB)  ( 331 ) 244 Effect of Sequential Chemotherapy Combined with 125I RadioactiveSeed Implantation on the Efficacy and Quality of Life of Patients with Recurrent Cervical Cancer CHEN Yongfu, HE Yaowu, LI Jian DOI: 10.3969/j.issn.1006-6233.2020.02.017 Objective: To investigate the effect of sequential chemotherapy combined with 125I radioactive seed implantation on the efficacy and quality of life of patients with recurrent cervical cancer. Methods: 95 patients with recurrent cervical cancer admitted to our hospital from January 2012 to July 2019 were enrolled. According to the simple random stratification method, 50 cases were divided into study group and 45 cases in control group. Sequential chemotherapy (4 courses) was used in the control group, and the study group was treated with sequential chemotherapy combined with 125I radioactive seed implantation. One month after treatment, the clinical efficacy, clinical benefit response (CBR), and quality of life (QLQ-C30 score) were compared between the two groups. Results: The total effective rate (78.00%) and local control rate (90.00%) in the study group were higher than those in the control group (55.56%, 71.11%), and the difference was statistically significant (P<0.05). CBR analysis showed that the patients in the study group recovered significantly, the body mass increased significantly, and the pain degree was significantly reduced, accounting for 74.00%, 78.00%, and 82.00%, respectively, which was higher than the control group’s 46.67%, 51.11%, and 40.00%. The difference was statistically significant (P<0.05).There was no significant difference in the scores of QLQ-C30 between the two groups before treatment (P>0.05). After treatment, the single item and symptom area scores of the study group were lower than the control group, and the functional areas and overall health scores were higher than the control group. The difference was statistically significant (P<0.05). Conclusion: Sequential chemotherapy combined with 125I radioactive seed implantation can improve the therapeutic effect and clinical benefit rate of patients with recurrent cervical cancer, and promote the improvement of patients' quality of life. 2020 Vol. 26 (2): 244-247 [Abstract] ( 126 ) HTML (1 KB)  PDF (1254 KB)  ( 293 ) 248 Study on the Effect of Aripiprazole Combined with Olanzapine on Clinical Efficacy Glucose and Lipid Metabolism of Elderly Schizophrenic Patients CHEN Mingju, et al DOI: 10.3969/j.issn.1006-6233.2020.02.018 Objective: To study the effect of aripiprazole combined with olanzapine on the clinical efficacy, glucose and lipid metabolism of elderly schizophrenic patients. Methods: 120 patients with schizophrenia admitted to our hospital from January 2016 to February 2018 were selected as the research objects, and divided into the control group (60 cases) and the observation group (60 cases) according to the random number table. They were treated for eight weeks. Patients in both groups were given conventional psychiatric medication, while those in the control group were supplemented with aripiprazole on the basis of conventional treatment. The initial dose was set at 10~20 mg, and the dosage was adjusted according to the actual condition of patients. The observation group was supplemented with olanzapine on the basis of treatment of the control group and the starting dose was set at 10~20 mg. The duration of treatment was 8 weeks. Results: The total clinical effective rate of the two groups after treatment was compared, the total clinical effective rate of the control group was 56.66%, the total clinical effective rate of the observation group was 96.66%, the total clinical effective rate of the control group was significantly lower than that of the observation group, the difference was statistically significant (P<0.05). After 8 weeks of treatment, blood lipid and blood glucose between the two groups were compared, and the observation group after treatment was lower than the control group. There was no significant difference in the calculation result of psychological function and material life between the two groups before treatment (P<0.05). After treatment, the quality of life of the two groups improved,but the improvement in quality of life before and after treatment in the observation group was significantly greater than that in the control group(P<0.05). Conclusion: Aripiprazole combined with olanzapine has a significant clinical effect on elderly patients with schizophrenia, has a significant effect on improving glucose and lipid metabolism, and can improve the quality of life of patients with schizophrenia. 2020 Vol. 26 (2): 248-251 [Abstract] ( 118 ) HTML (1 KB)  PDF (1247 KB)  ( 342 ) 252 Effects of Human Cytomegalovirus Infection on Glucose Metabolism C-peptide and Insulin Sensitivity in Patients with Type 2 Diabetes Mellitus BAI Yaobo, SHI Yan, LIU Wei DOI: 10.3969/j.issn.1006-6233.2020.02.019 Objective: To investigate the effects of human cytomegalovirus (HCMV) infection on glucose metabolism, C-peptide and insulin sensitivity in patients with type 2 diabetes mellitus (T2DM). Methods: 154 patients with T2DM admitted to the hospital during the period from February 2016 to February 2018 were included in the T2DM group. 50 healthy adults were included in the control group. The HCMV-DNA viral load, fasting plasma Glucose (FPG), C-peptide (C-P), fasting insulin (Fins), insulin sensitivity index (ISI) and T lymphocyte subsets in all subjects were determined and analyzed. Results: The positive rates of HCMV-DNA in the T2DM group and the control group were 50.64% and 26.00%, respectively. The HCMV-DNA load in the T2DM group was significantly higher than that in the control group (P<0.05). The FPG, ratio of CD4+ lymphocytes and CD4+/CD8+ ratio were increased, the Fins, CP, ISI and ratio of CD8+ T lymphocytes were decreased in the T2DM group, compared with the control group (P<0.05). The increase or increase of each index in HCMV-DNA positive patients was significantly greater than that in HCMV-DNA negative patients (P<0.05). Conclusion: HCMV infection can cause abnormal glucose metabolism in patients with T2DM, reduce insulin sensitivity, aggravate immune imbalance, and promote the progression of the disease. 2020 Vol. 26 (2): 252-254 [Abstract] ( 123 ) HTML (1 KB)  PDF (1229 KB)  ( 318 ) 255 Effect of Wenjing Huoxue Decoction on Hemorheology and Pain Index in Patients with Lumbar Disc Herniation LI Min, et al DOI: 10.3969/j.issn.1006-6233.2020.02.020 Objective: To investigate the effects of Wenjing Huoxuefang fumigation on hemorheology and pain index in patients with lumbar disc herniation. Methods: 96 patients with lumbar disc herniation admitted to our hospital from January 2015 to February 2018 were enrolled. The patients were divided into observation group and control group by random number table, 48 cases each. The control group was treated with traction and massage. The observation group was combined with Wenjing Huoxue Fumigation on the basis of this, and the blood rheology, waist activity index, activities of daily living and pain index were compared between the two groups. Results: The total effective rate of the observation group was significantly higher than that of the control group (P<0.05). After treatment, the VAS score, gait score, straight leg elevation test score, sensory disorder score, dyskinesia score, and daily activities were observed. The scores were significantly improved compared with those before treatment (P<0.05). The VAS scores in the observation group were lower than those in the control group. The gait score, the straight leg elevation test score, the sensory disorder score, the dyskinesia score, and the daily activity impact score were significantly higher. The control group (P<0.05); the lumbar activity index and activities of daily living were significantly higher in the observation group than in the control group (P<0.05). After treatment, the whole blood high-viscosity, Erythrocyte aggregation index, whole blood low-cut viscosity, erythrocyte rigidity index, plasma viscosity were significantly improved compared with before treatment, and the indicators of hemorheology in the observation group were lower than those in the control group (P<0.05). Conclusion: Wenjing Huoxuefang fumigation treatment can effectively improve the blood rheology of patients with lumbar disc herniation, improve the clinical symptoms and pain of patients, and the effect is good, it is recommended. 2020 Vol. 26 (2): 255-258 [Abstract] ( 112 ) HTML (1 KB)  PDF (1242 KB)  ( 270 ) 259 Comparison of the Effects between Biotype Artificial Femoral Head Replacement and PFNA Internal Fixation for the Treatment of Evans III and IV Intertrochanteric Fractures in the Elderly BO Shuxin, WANG Qian, CHEN Ping DOI: 10.3969/j.issn.1006-6233.2020.02.021 Objective: To investigate the clinical effects of biotype artificial femoral head replacement and proximal femoral nail anti-rotation (PFNA) internal fixation in the treatment of elderly Evans III and IV intertrochanteric fractures. Methods: 80 patients with intertrochanteric fractures diagnosed in the hospital from December 2015 to January 2018 were selected as study subjects. They were divided into the control group and the observation group according to the non-randomized clinical concurrent control study and patient voluntary principle, 40 patients in each group. The control group was treated with PFNA internal fixation while the observation group was treated with Corail biotype artificial femoral head replacement. The general conditions of surgery, clinical curative effect, hip function recovery (evaluated by Harris score) and complications were compared between the two groups. Results: The surgical time of the observation group was significantly longer than that of the control group, and intraoperative blood loss was more than that of the control group, while length of hospital stay and postoperative ambulation time were significantly shorter than those of the control group. The clinical total response rate of the observation group was significantly higher than that of the control group after treatment (P<0.05). There was no significant difference in preoperative Harris score between the two groups (P>0.05). The Harris scores of observation group were significantly higher than those of the control group at 1 month, 3 months and 6 months after surgery (P<0.05). The total incidence of postoperative complications in the observation group was significantly lower than that in the control group (P<0.05). Conclusion: Compared with PFNA internal fixation, biological artificial femoral head replacement has more advantages in the treatment of elderly patients with Evans III and IV intertrochanteric fractures in terms of efficacy, prognosis and safety. 2020 Vol. 26 (2): 259-263 [Abstract] ( 116 ) HTML (1 KB)  PDF (1927 KB)  ( 303 ) 270 Study on Changes of AngⅡ, ANP and MMP-9 in Elderly Patients with Chronic Heart Failure Treated with Digoxin Tablets ZHU Xiaoqin, DENG Rensheng DOI: 10.3969/j.issn.1006-6233.2020.02.024 Objective: To analyze the effect of digoxin tablet on angiotensin Ⅱ (Ang Ⅱ) and atrial natriuretic peptide (ANP), matrix metalloprotease 9 (MMP-9) in elderly patients with chronic heart failure. Methods: 128 elderly patients with chronic heart failure were enrolled in our hospital from February 2015 to March 2018. They were divided into two groups according to different treatment methods. The control group was given routine treatment, the observation group was treated with digoxin tablets, and the clinical effect of the two groups was analyzed. Results: There was no significant difference in cardiac function, ang Ⅱ, ANP, MMP-9, TIMP-l, ET-1, cat and GSH PX between the two groups (P>0.05). The level of (53.26 ± 8.67) ng / l was lower than that of the control group (P<0.05). There was no significant difference between the observation group (9.38%) and the control group (4.69%) (P>0.05). Conclusion: Digoxin tablet can reduce the level of ANP, MMP-9, ang Ⅱ, improve the heart function, reduce the oxidative stress response, and does not increase the adverse reactions. 2020 Vol. 26 (2): 270-274 [Abstract] ( 107 ) HTML (1 KB)  PDF (1237 KB)  ( 253 ) 275 Analysis on the Effects and Safety of Huangqi Capsule Assisted with Cefoperazone-sulbactam and Tigecycline in the treatment of MDRAB Pneumonia YANG Shuai, ZHANG Mingyue, KANG Mengjie, et al DOI: 10.3969/j.issn.1006-6233.2020.02.025 Objective: To explore the effects and safety of Huangqi capsule combined with cefoperazone-sulbactam and tigecycline in the treatment of multidrug-resistant Acinetobacter baumannii (MDRAB) pneumonia. Methods: The clinical data of 52 cases of MDRAB pneumonia treated with cefoperazone sulbactam + tegacyclin (control group) and Huangqin capsule + cefoperazone sulbactam + tegacyclin (observation group) were analyzed retrospectively. The bacterial clearance, state of recovery (time of normal temperature recovery, Rale disappearance, lung shadow disappearance) and adverse reactions were recorded, and the infection status (PCT, MMP-9), TCM syndrome (tcmsss), lung injury (lung injury) were compared before and after treatment for 2 weeks Score (LIS)] difference. Results: The bacterial clearance in observation group was significantly better than that in control group (P<0.05), and the normal recovery time of body temperature, disappearance time of rales, disappearance time of lung shadow and total incidence rate of adverse reactions during treatment were significantly lower than those in control group (P<0.05). After 2周 of treatment, the scores of infection status (serum PCT, MMP-9), TCM syndrome (TCMSSS score) and lung injury (LIS score) were lower than those before treatment (P<0.05), and the scores in observation group were lower than those in control group (P<0.05). Conclusion: Huangqi capsule assisted with cefoperazone-sulbactam and tigecycline has significant effects in the treatment of MDRAB pneumonia, and it can also reduce the risk of adverse reactions and has high clinical application value. 2020 Vol. 26 (2): 275-277 [Abstract] ( 131 ) HTML (1 KB)  PDF (1220 KB)  ( 421 ) 278 Application of Bronchial Plug in Da Vinci Robot Thymoma Resection YU Zhiyang, LIU Yang, HUI Kangli, et al DOI: 10.3969/j.issn.1006-6233.2020.02.026 Objective: To observe the clinical effect of bronchial occluder in Thymectomy with the robot system of Leonardo da Vinci (rats). Methods: 48 patients who underwent thymectomy in our hospital from January 2018 to April 2019 were selected and included in this study. According to the different intubation methods, they were divided into two groups: double lumen endotracheal tube group (DLT group, n = 24) and bronchial occluder group (BBS group, n = 24). The anesthesia and operation methods of the two groups were basically the same, and the hands of the two groups were recorded The peak airway pressure (ppeak), PO2 and PCO2 were recorded at four time points before one lung ventilation (T1), 10 minutes after one lung ventilation (T2), 60 minutes after one lung ventilation (T3), and 10 minutes after two lung ventilation (T4), respectively. The voice hoarseness and throat pain and the incidence of complications were recorded. Results: There was no significant difference in the success rate of intubation and satisfaction rate of pulmonary collapse between the two groups (P>0.05). The intubation time and pulmonary collapse time of BBS group were longer than those of DLT group (P<0.05), and the rate of lateral displacement tube was higher than that of DLT group (P<0.05). The peak value of BBS group at T2 and T3 was lower than that of DLT group (P<0.05), but the pO2 and PCO2 of the two groups at T1, T2, T3 and T4 were poor The incidence of hoarseness and pharyngeal pain in BBS group was lower than that in DLT group (P<0.05). Conclusion: In Da Vinci's robotic thymectomy, the bronchial occluder has the advantages of low airway resistance and low incidence of postoperative pharyngeal pain. The patient's comfort is higher, but the time of lung collapse is longer. Compared with the double lumen bronchial tube, the two groups have similar effects and advantages. 2020 Vol. 26 (2): 278-281 [Abstract] ( 133 ) HTML (1 KB)  PDF (1240 KB)  ( 322 ) 282 Study on the Correlation between Serum 25(OH)D Level and Thyroid Function Status During Pregnancy XIAO Min, SU Yan, SUN Chunping DOI: 10.3969/j.issn.1006-6233.2020.02.027 Objective: To explore the correlation between serum 25-hydroxyvitamin D [25(OH)D] level and thyroid function status during pregnancy. Methods: 80 patients with subclinical hypothyroidism (SCH) admitted between June 2017 to April 2018 were enrolled in SCH group. Another 70 healthy pregnant women were selected as control group. The levels of serum 25(OH)D, thyroid peroxidase antibody (TPOAb) free triiodothyronine (FT3), thyroid stimulating hormone (TSH) and free thyroxine (FT4) were detected at 24 to 28 weeks of gestation, and the relationship between 25(OH)D and thyroid function indexes was analyzed. Results: The level of serum TSH and positive rate of TPOAb in SCH group were significantly higher than those in control group (P<0.05) while the levels of serum FT3 and 25(OH)D in SCH group were significantly lower than that in control group (P<0.05), and there was no significant difference in FT4 level between the two groups (P>0.05). The vitamin D deficiency rate in SCH group was significantly higher than that in control group (P<0.05). The levels of serum TSH and FT3 and positive rate of TPOAb in deficiency group were significantly higher than those in insufficiency group (P<0.05), and there was no significant difference in the serum FT4 level between the two groups (P>0.05). 25(OH)D was negatively correlated with the levels of TSH and TPOAb (P<0.05), and 25(OH)D was positively correlated with FT3 level (P<0.05). Conclusion: Vitamin D deficiency in women with advanced pregnancy is closely related to SCH. Vitamin D deficiency and TPOAb positive may be important factors for SCH in women during pregnancy. 2020 Vol. 26 (2): 282-285 [Abstract] ( 115 ) HTML (1 KB)  PDF (1242 KB)  ( 210 ) 286 Effects of Ultra-early Application of Alteplase Intravenous Thrombolysis on Clinical Outcomes of Patients with Cerebral Infarction WANG He, SUN Yuanlin DOI: 10.3969/j.issn.1006-6233.2020.02.028 Objective: To explore the clinical effects of ultra-early application of alteplase intravenous thrombolysis on cerebral infarction and its influence on clinical outcomes. Methods: From January 2017 to January 2019, 96 patients with cerebral infarction in our hospital were selected. According to different treatment methods, they were divided into control group and study group, 48 cases in each group. Control group was given combined treatment of Bayaspirin enteric-coated tablets, clopidogrel hydrogen sulphate tablets and Shuxuetong injection while study group was given ultra-early application of alteplase intravenous thrombolysis, and given routine administration of Bayaspirin enteric-coated tablets and clopidogrel hydrogen sulphate tablet after 24hs, and method and dose in study group were the same as control group, and they were treated for 2 weeks. The neurological deficit, daily living ability, inflammatory factors levels, central nervous system-specific protein level, neuro-specific enolase level, coagulation function and adverse reactions after administration were compared between the two groups. Results: There were no adverse reactions in study group, and there was no significant difference compared with 2.08% in control group (P>0.05). The neurological deficit score, Barthel index score, S100β level, NSE level and various inflammatory factors in the two groups were effectively improved after treatment, and the improvements in study group were better than those in control group (P<0.05). After treatment, the plasma fibrinogen FIB of the two groups was significantly decreased (P<0.05). However, there were no significant differences in the coagulation function indexes between the two groups (P<0.05). Conclusion: Ultra-early alteplase intravenous thrombolysis has reliable effects and good safety in the treatment of patients with cerebral infarction, thus it is worthy of promotion. 2020 Vol. 26 (2): 286-289 [Abstract] ( 138 ) HTML (1 KB)  PDF (1237 KB)  ( 318 ) 290 Analysis on the Correlation between the Levels of TNF-alpha, IL-6 and IL-8 in Synovial Fluid and X-ray Classification and Joint Function in Patients with Traumatic Arthritis WU Xu, YAO Mengwei, GUO Zhen DOI: 10.3969/j.issn.1006-6233.2020.02.029 Objective: To analyze the correlation between the levels of inflammatory factors in synovial fluid and X-ray classification and knee function in patients with traumatic arthritis. Methods: 85 patients with traumatic arthritis (case group) and 45 health examinees (control group) were selected from January 2017 to January 2019. The levels of interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) in the synovial fluid of the two groups were compared. The differences of IL-6, IL-8 and TNF-α level in synovial fluid of patients with different X-ray grades were compared. Pearson correlation was used to analyze the relationship between KSS, HSS and IL-6, IL-8 and TNF-α level in synovial fluid. Results: The levels of IL-6, IL-8 and TNF-alpha in the synovial fluid of the case group were significantly higher than those of the control group (P<0.01). X-ray examination showed that there were 35 cases of grade I, 21 cases of grade II, 19 cases of grade III and 10 cases of grade IV. The levels of IL-6, IL-8 and TNF-α in synovial fluid of patients with different X-ray grading were significantly different (P<0.01), suggesting that the levels of IL-6, IL-8 and TNF-α in synovial fluid would increase with the increase of X-ray grading and the aggravation of bone injury. The average KSS score and HSS score were (63.47±10.46) and (67.98±13.28) respectively. Pearson correlation analysis showed that KSS score and HSS score at admission were negatively correlated with IL-6, IL-8 and TNF-α in synovial fluid (P<0.01), but not with ages and body mass index (P>0.05). Conclusion: The levels of inflammatory factors (IL-6, IL-8, TNF-α) in synovial fluid are closely related to X-ray classification and knee function in patients with traumatic arthritis. With the increase of X-ray classification, knee function is significantly weakened, and the levels of inflammatory factors (IL-6, IL-8, TNF-α) in synovial fluid are significantly increased. 2020 Vol. 26 (2): 290-293 [Abstract] ( 127 ) HTML (1 KB)  PDF (1246 KB)  ( 291 ) 294 Comparative Study on the Effect of Non Bracket Invisible Appliance and Straight Wire Appliance in the treatment of Periodontitis with Malocclusion SHI Min, WANG Hong, et al DOI: 10.3969/j.issn.1006-6233.2020.02.030 Objective: To explore the effect of bracketless invisible appliance and straight wire appliance in the treatment of periodontitis with malocclusion. Methods: 90 patients with periodontitis and malocclusion from January 2018 to January 2019 were randomly divided into two groups, 45 in each group. The control group was treated with straight wire appliance, while the observation group was treated with non-bracket invisible appliance. The periodontal index, orthodontic effect and complication rate were compared between the two groups. Results: The periodontal indexes of the observation group were lower than those of the control group after 6 months of treatment, with statistical significance (P<0.05); there was no significant difference in the orthodontic effect between the two groups after 6 months of treatment (P>0.05); the incidence of complications in the observation group was lower than that in the control group after 6 months of treatment, with statistical significance (P<0.05). Conclusion: In the treatment of periodontitis patients with malocclusion, the application of non bracket invisible appliance can effectively reduce the periodontal index of patients, significantly reduce the incidence of complications, which is worthy of promotion. 2020 Vol. 26 (2): 294-297 [Abstract] ( 103 ) HTML (1 KB)  PDF (1242 KB)  ( 337 ) 298 Effects of MRI Scan Combined with Serum GP73 and AFP Levels on the Diagnostic Efficacy of Primary Hepatic Carcinoma ZHOU Ting, WANG Gang, ZHANG Jun DOI: 10.3969/j.issn.1006-6233.2020.02.031 Objective: To study the effects of MRI scan combined with serum GP73 and AFP levels on the diagnostic efficacy of primary hepatic carcinoma (PHC). Methods: 92 patients with suspected PHC from June 2018 to June 2019 in our hospital were selected for the study. According to the results of pathological examination, the patients were divided into PHC group (47 cases) and benign group (45 cases). 50 healthy examiners in our hospital at the same period were selected as control group, and the MRI scan was performed, and the expression levels of serum alpha-fetoprotein (AFP) and Golgi glycoprotein 73 (GP73) were measured and compared. Kappa consistency test was used to analyze the value of each method for the diagnosis of PHC alone or in combination. Results: MRI showed 48 patients with PHC, including 26 cases of nodular type, 17 cases of massive type and 5 cases of diffuse type. T1WI mainly showed even low or slightly low signal, and T2WI mainly showed uneven high signal, and DWI showed obviously enhanced tumor tissues, and the signal intensity was higher than that of surrounding normal tissues, and some cases showed high signal necrosis and low signal hemorrhage. The ADC value in PHC group was significantly lower than that in benign group and control group (P<0.05), and the levels of serum AFP and GP73 were significantly higher than those in benign group and control group (P<0.05), and the ADC value in benign group was significantly lower than that in control group (P<0.05), and the levels of serum AFP and GP73 were significantly higher than those in control group (P<0.05). Taking pathological results as the gold standard, and consistency Kappa test showed that levels of serum AFP and GP73 had lower diagnostic efficacy for PHC (Kappa value <0.5), and combined with MRI examination could effectively improve the diagnostic efficacy (Kappa value 0.5~0.7), and MRI had good diagnostic value for PHC (Kappa value>0.7), and the combined diagnostic accuracy of the three could be further improved (Kappa value > 0.7). Conclusion: MRI scan combined with serum GP73 and AFP levels can effectively improve the diagnostic accuracy of PHC, and it is of great significance for early treatment of PHC and improvement of prognosis. 2020 Vol. 26 (2): 298-301 [Abstract] ( 116 ) HTML (1 KB)  PDF (1246 KB)  ( 314 ) 302 Exploration of Efficacy of Golimumab Combined with Leflunomide in the treatment of Active RA with Poor MTX Efficacy PAN Lu, ZHAO Hua, et al DOI: 10.3969/j.issn.1006-6233.2020.02.032 Objective: To study the effects of golimumab combined with leflunomide in the treatment of active rheumatoid arthritis (RA) with poor treatment effects of methotrexate (MTX). Methods: 70 patients with active RA with poor MTX treatment effects in the hospital from December 2016 to December 2018 were divided into observation group (golimumab +leflunomide, n=35) and control group (leflunomide, n=35) according to the simple random grouping. The treatment effects were observed in the two groups. Results: After treatment, the morning stiffness time in the two groups was significantly shortened, and the quantities of joint swelling and tenderness were significantly decreased (P<0.05), and the morning stiffness time and quantities of joint swelling and tenderness in observation group were significantly shorter/Less than those in control group (P<0.05). After treatment, the visual analogue pain scale (VAS) scores of patients and doctors in the two groups were significantly lower than those before treatment (P<0.05), and the VAS scores of patients and doctors in observation group after treatment were significantly lower than those in control group (P<0.05). After treatment, the levels of erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and rheumatoid factor (RF) were significantly lower than those before treatment (P<0.05), and the levels of above substances in observation group were significantly lower than those in control group (P<0.05). After treatment, the levels of serum interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), plasma fibrin/fibrinogen degradation product (FDP) and D-dimer (DD) were significantly lower than those before treatment (P<0.05), and the levels of above substances in observation group after treatment were significantly lower than those in control group (P<0.05). The proportions of ARC50 and ARC70 in observation group were significantly higher than those in control group (P<0.05). There were no significant differences in the adverse drug reactions between the two groups (P>0.05). Conclusion: Golimumab combined with leflunomide has good treatment effects in active RA patients with poor MTX treatment effects. And it can effectively alleviate clinical symptoms such as morning stiffness, joint swelling and tenderness, reduce the levels of ESR, CRP, RF and serum-related inflammatory substances, and improve the treatment effects, and it does not increase the adverse reactions, thus it is worthy of clinical promotion. 2020 Vol. 26 (2): 302-306 [Abstract] ( 125 ) HTML (1 KB)  PDF (1259 KB)  ( 402 ) 307 Retrospective Analysis of the Risk Factors and the Causes of short-term Death after Severe Abdominal Trauma LIU Shihui, LV Liangfeng, HU Chengwen DOI: 10.3969/j.issn.1006-6233.2020.02.033 Objective: To analyze the causes and risk factors of short-term death after severe abdominal trauma. Methods: The clinical data of 80 patients with severe abdominal injuries were retrospectively analyzed. According to the outcome, the patients were divided into death group and survival group. The cause of death was analyzed. The causes of death of the patients were analyzed, and the data of gender, age, time from injury to admission, operation time, injury classification, assessment results of injury at admission, detection results of relevant indicators, and whether cardiopulmonary resuscitation was adopted were compared between the two groups. Multivariate Logistic regression analysis was used to identify the risk factors for short-term death after severe abdominal trauma. Results: 15 of 80 patients died (18.75%). Causes of death: central respiratory and circulatory failure in 6 cases (40.00%), traumatic shock in 4 cases (26.67%); acute respiratory distress syndrome in 3 cases (20.00%); multiple organ failure in 2 cases (13.33%). The proportion of patients with age > 60 years old, multiple injuries, ISS score ≥ 16 was significantly higher than that of patients with survival (P<0.05), while the body temperature of patients with death at admission was significantly lower than that of patients with survival, Apache Ⅱ score, alkali surplus, Pt, and significantly higher than that of patients with survival (P<0.05). Logistic multiple regression analysis showed that age, apcahe Ⅱ score, multiple injuries and ISS score were independent risk factors for short-term death after severe abdominal trauma (P<0.05). Conclusion: The cause of death in patients with severe abdominal trauma is central respiratory and circulatory failure and traumatic shock, and is closely related to patient age, APCAHE II score, multiple trauma, and ISS scores. 2020 Vol. 26 (2): 307-310 [Abstract] ( 234 ) HTML (1 KB)  PDF (1239 KB)  ( 756 ) 311 Comparative Study of Standard Large Flap and Conventional Flap Decompression in the Treatment of Severe Craniocerebral Injury LI Qing, LI Weidong, DING Yuan, et al DOI: 10.3969/j.issn.1006-6233.2020.02.034 Objective: To observe the therapeutic effect of standard large bone flap and conventional bone flap decompression on patients with severe craniocerebral injury. Methods: 90 patients with severe craniocerebral injury were divided into observation group and control group according to random number table method, 45 cases in each group. The observation group was treated with standard large bone flap decompression combined with dural reticulotomy, while the control group was treated with conventional bone flap decompression combined with radial dural incision. Intracranial pressure (ICP), brain metabolic index, disturbance of consciousness, prognosis of disease and ability of daily living after treatment were compared between the two groups. Results: The ICP, CCP, SVO2, GCS, GOS and Bathel of the patients in the observation group were better than those in the control group (P<0.05). Conclusion: Compared with conventional craniotomy and craniotomy, standard large craniotomy combined with duratomy can effectively reduce intracranial pressure, promote brain metabolism and improve the prognosis of severe craniocerebral injury. 2020 Vol. 26 (2): 311-314 [Abstract] ( 101 ) HTML (1 KB)  PDF (1248 KB)  ( 316 ) 315 Risk Factors for Wrist Joint Recovery after Traumatic Distal Radial Fracture Fixation LI Jili, YAO Heng, WANG Wei, et al DOI: 10.3969/j.issn.1006-6233.2020.02.035 Objective: To explore the risk factors of wrist joint recovery after traumatic distal radius fracture fixation. Methods: From August 2011 to October 2017, 98 patients with traumatic distal radius fracture were selected and divided into good group and bad group according to the recovery effect of wrist joint 6 months after operation. The clinical characteristics of the two groups were compared, including sex, age, body mass index, time of treatment, osteoporosis, fracture type, fracture AO classification, injury energy, fracture of styloid process of ulna, last reexamination of palm angle, last reexamination of radial shortening and functional exercise. Logistic regression model was used to analyze the risk factors of poor wrist joint recovery after fixation of traumatic distal radius fracture. Results: Among the 98 patients, 26 cases recovered poorly, accounting for 26.53%, 72 cases recovered well, accounting for 73.47%. The proportion of irregular exercise was 53.85%, 65.38%, 57.69%, 46.15%, 46.15% and 42.31% in the poor group, which was higher than 31.94%, 15.28%, 16.67%, 16.67%, 15.28%, 9.72% in the good group (P<0.05). Logistic regression analysis showed that age (> 65 years), osteoporosis, comminuted fracture, palmar tilt angle at the last reexamination (< 10 degrees), radial shortening (> 5mm) at the last reexamination, and functional exercise (irregular) were the risk factors for poor wrist recovery (P<0.05). Conclusion: The poor recovery of wrist joint after internal fixation of traumatic distal radius fracture is closely related to age ≥ 65 years old, osteoporosis, comminuted fracture, palm angle < 10 度, radius shortening ≥ 5mm and irregular functional exercise. 2020 Vol. 26 (2): 315-319 [Abstract] ( 129 ) HTML (1 KB)  PDF (1563 KB)  ( 426 ) 320 Application of 3D Printing Navigation Internal Fixation for Femoral Neck Fracture GU Yang, CHEN Jin, ZHU Xijun, et al DOI: 10.3969/j.issn.1006-6233.2020.02.036 Objective: To design the hollow nail internal fixation channel and operation navigation device for the patients with femoral neck fracture before operation, and discussed its application value by 3D printing navigation internal fixation technology. Methods: 86 cases with femoral neck fracture admitted in our hospital from January 2015 to June 2017 were randomly divided into the observation group and the control group, with 43 cases in each group. All cases were treated with closed reduction and cannulated screw internal fixation. Patients in the control group received routine operation scheme, while for patients in the observation group, nail path position and navigator were designed by preoperative 3D printing navigation fixation technology. The basic indexes of surgery, postoperative screw distribution, Harris hip function score at 3 and 12 months after surgery, fracture healing status at the end following-up were observed and compared in 2 groups. Results: The duration from admitting to surgery and the duration of surgery in the observation group were much longer than those in the control group, the amount of bleeding during surgery in the observation group was much more than that in the control group, the times of fluoroscopy and of nail piercing in the observation group were much less than those in the control group, the differences were statistically significant (P＜ 0.05). The parallel degree of screws at front and side in the observation group were significantly lower than that in the control group, and the ratio of screw distribution area in the observation group was significantly higher than that in the control group (P＜ 0.05). At 3 months after operation, the Harris hip function score in the observation group was much better than it in the control group (P＜ 0.05). There were no significant differences in Harris hip function score between the two groups at 12 months after operation (P＞ 0.05). There was no significant difference in incidence of fracture nonunion, femoral head necrosis, Garden indexes and Harris hip function score at the end of following-up (P＞ 0.05). Conclusion: Nail path position and navigator designed by preoperative 3D printing navigation fixation technology can effectively guide cannulated screw internal fixation for patients with femoral neck fracture. It can reduce the frequency of fluoroscopy and of nail piercing, and will not increase the risk of adverse prognosis. It helps patients perform functional exercises as early as possible. 2020 Vol. 26 (2): 320-324 [Abstract] ( 156 ) HTML (1 KB)  PDF (2192 KB)  ( 358 ) 329 Effect of Juglone on Proliferation and Apoptosis of Oral Squamous Cell Carcinoma Tca8113 Cells by Regulating CDK4 Expression WU Jian'an, SHANG Xia, HU Qian DOI: 10.3969/j.issn.1006-6233.2020.02.038 Objective: To investigate effect of juglone on proliferation and apoptosis of oral squamous cell carcinoma Tca8113 cells by regulating CDK4 expression. Methods: Oral squamous cell carcinoma Tca8113 cells were divided into 4 groups: control group, low-dose, middle-dose, high-dose juglone groups (0μmoL/L, 25μmoL/L, 50μmoL/L, 100μmoL/L). The morphological changes of cells in different concentration groups were observed by inverted microscope.The inhibitory effect of juglone on the proliferation of Tca8113 cells was determined by MTT assay. The effect of walnut juglone on Tca8113 cell cycle distribution was analyzed by flow cytometry. The expression of CDK4 in Tca8113 cells was analyzed by immunocytochemistry. Results: 24 hours after the action of walnut quinone, the number of cells was decreased, the cytoplasm became rough, the cells became smaller and rounder, and the higher the concentration of walnut quinone, the more obvious the change was. MTT results showed that OD values of Tca8113 cells in low, medium and high dose groups were lower than those in control group (P<0.05), OD values of Tca8113 cells in medium and high dose groups were lower than those in low dose group (P<0.05), apoptotic rates in high, medium and low dose groups were significantly higher than those in control group (P<0.05), and apoptotic rates in high, medium and low dose groups were higher than those in low dose group (P<0.05). The positive rate of CDK4 in high-dose group was higher than that in medium-dose group (P<0.05); the positive rate of CDK4 in high, middle and low-dose group was lower than that in control group (P<0.05); the positive rate of CDK4 in high and middle-dose group was lower than that in low-dose group (P<0.05); and the positive rate of CDK4 in high-dose group was lower than that in middle-dose group (P<0.05). Conclusion: Juglone can inhibit the proliferation of oral squamous cell carcinoma Tca8113 cells, induce apoptosis, down-regulate the expression of CDK4 protein, and inhibit the invasion of tumors. 2020 Vol. 26 (2): 329-332 [Abstract] ( 98 ) HTML (1 KB)  PDF (1461 KB)  ( 349 ) 333 Effect of Shenshao Oral Liquid on CD14 / TLR4 Mediated Secondary Inflammatory Injury in the Brain of Rats with Experimental Cerebral Hemorrhage LIU Hongwei, TANG Xingguo, LI Binbin, et al DOI: 10.3969/j.issn.1006-6233.2020.02.039 Objective: To research Shenshao Oral Liquid Inhibits Secondary Inflammatory Injure Induced by CD14/TLR4 after Intracerebral Hemorrhage in Experimental Rats. Methods: Experimental rats were randomly divided into sham operation group, model group and intervention group. Each group further was subdivided into 6 h group, 12 h group, 24 h group, 72 h group, and 7 d group. ICH group and SS group were used to duplicate the model of cerebral hemorrhage. SS group was given Shenshao oral liquid (8ml/d) by gavage after waking up. The number and protein expression of CD14, TLR4 and IL-8 positive cells were detected by immunohistochemistry and Western blotting. Results: The edema was appeared after 6h after the ICH model was established, and the water content of brain was gradually increased at 12h to 72h, and reached to the highest level at 72h, then the brain edema was gradually decreased, and ultimately got right original level at 7d. Compared with the ICH group, the water content of brain in the rats of the intervention group was obviously decreased (P<0.05). The expression of CD14 in brain tissue cells of sham group was slight; CD14 protein began to express around hematoma in ICH group at 6h after operation, and a small number of positive cells increased with time, reached the peak at 72h, and then decreased gradually. The trend of TLR4 and IL-8 protein expression was consistent with that of CD14. The expression level of TLR4 and CD14 was positively correlated with brain water content (P<0.05), and the expression level of TLR4 and IL-8 was significantly decreased after Shenshao oral liquid. Conclusion: Shenshao oral liquid may reduce the inducing factors of CD14 / TLR4 Signal pathway, reduce the secondary inflammatory injury of brain tissue around hematoma after cerebral hemorrhage, and play a protective role in brain tissue. 2020 Vol. 26 (2): 333-337 [Abstract] ( 145 ) HTML (1 KB)  PDF (1806 KB)  ( 386 ) 338 Analysis of Detection Value of Serum Anti-bFGF Autoantibodies in Active Autoimmune Aiseases MAO Hongbo, et al DOI: 10.3969/j.issn.1006-6233.2020.02.040 Objective: To investigate the expression significance of serum anti-basic fibroblast growth factor (bFGF) autoantibodies in patients with active autoimmune diseases. Methods: The clinical data of 139 patients with active autoimmune diseases were retrospectively analyzed. 46 healthy subjects who were admitted to the hospital for physical examination at the same time were used as control group. The levels of serum anti-bFGF-immunoglobulin (Ig)G antibody and anti-bFGF-IgM antibody were compared among patients with different types of diseases and kidney damage, and the correlation between the above indicators and urinary albumin excretion rate (UAER) and its predictive value on kidney damage were analyzed by Pearson correlation coefficient model and receiver operating characteristic (ROC) curve respectively. Results: The levels of serum anti-bFGF-IgG antibody and anti-bFGF-IgM antibody in patients with active systemic lupus erythematosus (SLE), Sjogren's syndrome (SS), rheumatoid arthritis (RA) and other types of autoimmune diseases were significantly higher than those of healthy subjects, and the levels in RA patients were significantly higher than those in SLE patients (P<0.05). The levels of serum anti-bFGF-IgG antibody, anti-bFGF-IgM antibody and UAER in patients with autoimmune diseases and kidney damage were significantly higher than those of patients with non-kidney damage (P<0.05). Levels of serum anti-bFGF-IgG antibody and anti-bFGF-IgM antibody in patients with active autoimmune diseases showed a significant positive correlation with UAER level (r>0, P<0.05). There was nos ignificant difference in the area under ROC curve (AUC) of serum anti-bFGF-IgG antibody and anti-bFGF-IgM antibody in predicting kidney damage in patients with active autoimmune diseases (P>0.05), and the prediction sensitivities were 83.33% and 91.67%, ans the specificities were 64.08% and 61.17%, and the cut-off values were 208.01×10-3 and 224.79×10-3 respectively. Conclusion: The expressions of anti-bFGF autoantibodies are significantly increased in patients with active autoimmune diseases. Clinical testing can provide a reference for disease assessment and lay a foundation for pathological mechanism research. 2020 Vol. 26 (2): 338-341 [Abstract] ( 102 ) HTML (1 KB)  PDF (1349 KB)  ( 309 ) 342 Relationship between Cytomegalovirus Infection and Serum TNF-alpha IL-1 and IL-6 in Spontaneous Abortion Patients ZHENG Chunjiao, WU Zhiming, HUANG Sujing, et al DOI: 10.3969/j.issn.1006-6233.2020.02.041 Objective: To explore the relationship between cytomegalovirus infection and serum TNF-alpha, IL-1 and IL-6 in patients with spontaneous abortion. Methods: The clinical data of 110 cases of spontaneous abortion pregnant women (observation group) and 70 cases of normal pregnant women (control group) were collected from January 2017 to January 2019. The observation group was divided into two subgroups according to the detection results of HCMV-IgM, HCMV-DNA and HCMV-RNA in peripheral blood, and either of them was positive. For HCMV active infection, 26 cases were active infection and 84 cases were inactive infection. The positive rates of HCMV-IgM, HCMV-DNA and HCMV-RNA, the levels of inflammatory cytokines (TNF-a, IL-1, IL-6) and the levels of inflammatory cytokines in active infection group and inactive infection group were compared between the observation group and the control group. The relationship between HCMV active infection and TNF-a, IL-1, IL-6 was analyzed. Results: The positive rates of HCMV-IgM, HCMV-DNA and HCMV-RNA in the observation group were 37.37%, 16.36% and 2364% respectively, which were significantly higher than those in the control group (7.14%, 4.29% and 5.71%), the differences were statistically significant (P<0.05). The serum levels of TNF-alpha, IL-1 and IL-6 in active infection group were higher than those in inactive infection group, the differences were statistically significant (P<0.05), it is suggested that serum TNF - α, IL-1 and IL-6 levels are related to active infection, and the higher the level, the higher the risk of active infection. Conclusion: HCMV infection is one of the important causes of spontaneous abortion, and HCMV infection is related to the levels of TNF - α, IL-1 and IL-6. The higher the level, the higher the risk of active infection, may play an important role in the occurrence of abortion. 2020 Vol. 26 (2): 342-345 [Abstract] ( 133 ) HTML (1 KB)  PDF (1236 KB)  ( 278 )

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