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2020 Vol. 26, No. 1 Published: 2020-01-31 1 Analysis on Correlation between Serum ox-LDL IL-18 and Lp-PLA2 Levels and Carotid Atherosclerotic Plaques in Patients with Cerebral Infarction ZHANG Jun, LU Zuneng DOI: 10.3969/j.issn.1006-6233.2020.01.001 Objective: To analyze the correlation between serum oxidized low density lipoprotein (oxidized low density lipoprotein, ox-LDL), interleukin-18 (IL-18), lipoprotein-associated phosphatase A2 (Lp-PLA2) and carotid atherosclerotic plaque in patients with cerebral infarction. Methods: 120 patients with cerebral infarction (study group) and 35 healthy volunteers (control group) who were admitted to the hospital from March 2018 to March 2019 were enrolled in the study. General data of both groups were collected. Serum ox-LDL, IL-18 and Lp-PLA2 levels were compared between the two groups. Carotid intima-media thickness (CIMT) and the maximum area of plaques were measured. Crouse score of carotid plaques was calculated. The correlation between serum ox-LDL, IL-18 and Lp-PLA2 levels and carotid atherosclerotic plaques was analyzed. Results: Serum ox-LDL, IL-18 and Lp-PLA2 levels, CIMT, the maximum area of plaques, Crouse score of carotid plaques in the study group were significantly higher than those of the control group (P<0.05). Correlation analysis showed that CIMT, the maximum area of plaques and Crouse score were positively correlated with serum ox-LDL, IL-18 and Lp-PLA2 levels (P<0.05). Conclusion: The changes of serum ox-LDL, IL-18 and Lp-PLA2 levels may be effective predictors of carotid atherosclerotic plaque formation in patients with cerebral infarction. 2020 Vol. 26 (1): 1-3 [Abstract] ( 295 ) HTML (1 KB)  PDF (1187 KB)  ( 388 ) 4 Effect of Rdiotherapy combined with EGFR-TKI Targeting Therapy on Serum Tumor Markers and Therapeutic Effect in Patients with Stage Ⅳ Non-small Cell Lung Cancer YANG Jiu, HONG Mei, LIU Zhiyuan, et al DOI: 10.3969/j.issn.1006-6233.2020.01.02 Objective: To explore the radiotherapy combined EGFR - TKI targeted therapy Ⅳ NSCLC serum tumor markers and therapeutic effect. Methods: From October 2017 to September 2018, 92 patients with stage Ⅳ NSCLC in our hospital were selected as the research objects. The treatment methods were divided into two groups, 46 cases in each group. The patients in each group were treated with radiotherapy alone and EGFR-TKI as the combined group. The clinical efficacy, serum biochemical indicators, immune function and toxic and side effects of the two groups were compared. Results: The total effective rate was 82.61% in the combined group, significantly higher than 54.35% in the radiotherapy group (χ2=8.51,P<0.05). Compared within the group, serum biochemical indexes of VEGF, CEA, CA125, MMP9 and CYFRA21-1 in the two groups were significantly decreased after treatment (all P＜0.05). After treatment, all biochemical indexes in the combined group were significantly lower than those in the radiotherapy group (P＜0.05). Immune function after treatment compared, CD4+ and NK level joint group were significantly higher than in radiotherapy group (P＜0.05). The CD8+ level was significantly lower than that of the radiotherapy group (P＜0.05). Conclusion: Radiotherapy combined with EGFR-TKI can improve the clinical therapeutic effect, effectively control tumor progression, improve immune function, and provide a better internal environment without increasing toxic and side effects. 2020 Vol. 26 (1): 4-7 [Abstract] ( 204 ) HTML (1 KB)  PDF (1212 KB)  ( 395 ) 8 The Value of Neutrophil/lymphocyte Ratio in Assessing the Severity and Prognosis of Ventilator-associated Pneumonia XIE Xiaobing, GAO Yun, LIU Kai, et al DOI: 10.3969/j.issn.1006-6233.2020.01.003 Objective: To evaluate the value of neutrophil/lymphocyte ratio in assessing the severity and prognosis of ventilator-associated pneumonia (VAP). Methods: The clinical data of 210 patients with VAP who were admitted to our hospital from December 2014 to December 2018 were analysized retrospectively. According to the acute physiology and chronic health evaluation II (APACHE II) within 24 hours, the patients were divided into low risk group (APACHE II score <10, 67 cases), intermediate risk group (10≤APACHE II score≤20, 70 cases) and high-risk group (APACHE II score > 20, 73 cases). According to clinical prognosis, they were divided into survival group (159 cases) and death group (51 cases). The clinical data of the patients in different groups were recorded including APACHE II score, clinical pulmonary infection score (CPIS), sepsis related organ failure assessment (SOFA), neutrophil (NEU), (lymphocyte (LYM), NLR, procalcitonin, (PCT), hypersensitive C reactive protein (hs-CRP). The logistics regression, correlation analysis and receiver operator characteristic curve (ROC) were used to analyze the data of different groups. Results: The difference of NLR levels among different severity groups was statistically significant (P<0.05), which high-risk group was the highest, intermediate risk group was the second, and low risk group was the lowest. The NLR level in the death group was significantly higher than that in the surviving group, the difference was statistically significant (P<0.05). The multivariate logistic regression analysis showed that NLR, prealbumin (PA), APACHE II, CPIS, SOFA were independent risk factors for poor prognosis. The correlation analsis showed that NLR level in the death group were positively correlated with APACHE II score, CPIS score, and SOFA score (r1=0.842, P1<0.001; r2=0.805, P2<0.001; r3=0.901, P3<0.001). There was a significant negative correlation between NLR and PA levels (r= -7.047, P<0.001). ROC curves analysis showed that the area under curve of NLR level was 0.833 (95%CI:0.735~0.931), and threshold (OOP) was 9.52, which had 81.58% sensitivity and 81.58% specificity in predicting poor prognosis. Conclusions: The NLR level has a high predictive value for assessing the severity and prognosis of patients with VAP, which is worthy of clinical promotion. 2020 Vol. 26 (1): 8-12 [Abstract] ( 225 ) HTML (1 KB)  PDF (1256 KB)  ( 448 ) 13 Changes of Serum IL-17 Th17 and Treg Cells in Patients with Acute Exacerbation of COPD before and after Treatment and the Correlation with Lung Function WU Guixiong, ZHU Shuiquan, DONG Shufeng, et al DOI: 10.3969/j.issn.1006-6233.2020.01.004 Objective: To observe the changes of serum interleukin-17 (IL-17), Th17 cells and regulatory T cells (Treg) in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) before and after treatment, and explore their correlation with lung function. Methods: 300 patients with AECOPD (AECOPD group) and 100 healthy controls (control group) were selected. The levels of serum Th17,Treg cells and IL-17 levels were detected by flow cytometry. and lung function was detected to obtain indicators such as the forced expiratory volume in 1s (FEV1), forced vital capacity (FVC) and FEV1/FVC. Results: Serum IL-17, Th17 cells and Th17/Treg ratio in AECOPD group were significantly higher than those in the control group (P<0.05). Serum IL-17, Th17 cells and Th17/Treg ratio in AECOPD group significantly decreased after treatment (P<0.05) while Treg cells increased significantly (P<0.05), with significant differences between groups (P<0.05). Pearson correlation analysis showed that serum Th17, IL-17 and Th17/Treg ratio in patients with AECOPD were negatively correlated with FEV1, FVC and FEV1/FVC before and after treatment (P<0.05) while Treg cells were positively correlated with FEV1, FVC and FEV1/FVC (P<0.05). ROC curve analysis showed that the areas under curves of serum Th17, Treg, Th17/Treg and IL-17 levels for predicting death of patients with AECOPD were 0.840, 0.812, 0.975 and 0.880, respectively. The area under the curve of Th17/Treg was the largest, and there were statistically significant differences, compared with the other three indexes (P<0.05). Conclusion: Serum IL-17, Th17 and Treg are closely related to lung function in patients with AECOPD. They can be used as important indicators for judgement of the condition and prognosis. Besides, the diagnostic efficiency of Th17/Treg is the highest. 2020 Vol. 26 (1): 13-17 [Abstract] ( 177 ) HTML (1 KB)  PDF (1331 KB)  ( 387 ) 18 Efficacy of Laparoscopic Radical Surgery combined with Intraperitoneal Hyperthermic Perfusion Chemotherapy on Advanced Rectal Cancer and Its Effects on Levels of Serum TK1, COX-2 and VEGF WU Lei, MENG Yong, CHENG Si DOI: 10.3969/j.issn.1006-6233.2020.01.005 Objective: To investigate the efficacy of laparoscopic radical surgery combined with intraperitoneal hyperthermic perfusion chemotherapy in the treatment of advanced rectal cancer and its effects on levels of serum thymidine kinase 1 (TK1), cyclooxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF). Methods: 100 patients with advanced rectal cancer in our hospital were observed. 43 patients in single group underwent laparoscopic radical surgery, and 57 patients in combined group underwent laparoscopic radical surgery combined with intraperitoneal hyperthermic perfusion chemotherapy. The therapeutic effects, occurrence of complications, recurrence, metastasis and survival, levels of serum TK1, COX-2 and VEGF and physical function status were observed in the two groups. Results: After treatment, the total effective rate in combined group was significantly higher than that in single group (78.95% vs 58.14%) (P<0.05). The incidence rates of anastomotic leakage, stump fistula, abdominal infection, intestinal obstruction and myelosuppression during treatment in combined group were not significantly different from those in single group (P>0.05). The recurrence rate and metastasis rate in combined group were significantly lower than those in single group while the 2-year survival rate was significantly higher than that in single group (P<0.05), and the progression-free survival rate in combined group was significantly higher than that in single group (P<0.05). After treatment, the decreases of serum TK1, COX-2 and VEGF in combined group were greater than those in single group (P<0.05). There were significant differences in the group effects, time effects and interaction effects of KPS (P<0.05), and the KPS scores at 1 year and 2 years after surgery in combined group were significantly higher than those in single group (P<0.05), and the KPS scores in the two groups were significantly increased at 1 year and 2 years after surgery (P<0.05). Conclusion: Laparoscopic radical surgery combined with intraperitoneal hyperthermic perfusion chemotherapy has exact efficacy and low incidence rate of complications in the treatment of advanced rectal cancer, and it can help reduce the tumor recurrence rate and metastasis rate, improve the survival, regulate the levels of serum TK1, COX-2 and VEGF and inhibit tumor cell proliferation, and it is conducive to the recovery of physical function. 2020 Vol. 26 (1): 18-22 [Abstract] ( 157 ) HTML (1 KB)  PDF (1323 KB)  ( 302 ) 23 Analgesic Effects of Lidocaine Combined with Ropivacaine Hydrochloride and Sufentanil Citrate on Self-controlled Epidural Labor of Puerperas and its Influence on Lactation Function WANG Zhanhui, LIU Yumei, LIU Xue DOI: 10.3969/j.issn.1006-6233.2020.01.006 Objective: To explore the analgesic effects of lidocaine combined with ropivacaine hydrochloride and sufentanil citrate on self-controlled epidural labor of puerperas and its influence on lactation function. Methods: 200 cases of primiparas with full-term pregnancy were randomly divided into two groups, namely, each 100 cases in observation group and control group. Control group was given natural delivery with whole-course accompany delivery model, and observation group was given painless delivery (self-controlled epidural labor analgesia of lidocaine + ropivacaine hydrochloride+sufentanil citrate). The analgesic effects, labor process, lactation and stress-related hormones levels were observed in the two groups. Results: There were significant differences in the between-group effects, time-point effects and interaction effects ofbetween-group and time-point of visual analogue scale (VAS) score between the two groups (P<0.05). Immediately after analgesia (T1), at 10min after analgesia (T2), at 30min after analgesia (T3), at 7~8 cm of uterus orifice opening (T4), at complete opening of uterus orifice (T5) and at fetal delivery time (T6), the VAS score in observation group showed a significant downward trend (P<0.05), and the scores in control group showed significant increase firstly and then significant decrease (P<0.05), and the scores at T1~T6 in observation group were significantly lower than those in control group (P<0.05). The active time in first stage of labor in observation group was significantly shorter than that in control group (P<0.05), and there were no significant differences in the first stage of labor latency, the second stage of labor and the third stage of labor between the two groups (P>0.05). The lactation starting time in observation group was significantly shorter than that in control group (P<0.05), and the lactation amount was significantly higher than that in control group (P<0.05). There were significant differences in the between-group effects, time-point effects and interaction effects of between-group and time-point of plasma prolactin (PRL) concentration between the two groups (P<0.05). At 24 h~48 h after delivery, the plasma PRL concentration in the two groups was increased significantly (P<0.05), and the increase in observation group was significantly greater than that in control group (P<0.05). After 30min of delivery, the levels of serum adrenal cortical hormone (ACTH), cortisol (COR), norepinephrine (NE) and epinephrine (E) were significantly decreased in observation group (P<0.05), and the levels were significantly decreased in control group (P<0.05), and the levels of above indicators in observation group were significantly lower than those in control group (P<0.05). Conclusion: The effect of lidocaine combined with ropivacaine hydrochloride and sufentanil citrate is better than that of traditional natural labor. It can obviously reduce labor pain, shorten labor process, reduce stress response, improve lactation function, and achieve the effect of early lactation and multiple lactation. 2020 Vol. 26 (1): 23-27 [Abstract] ( 170 ) HTML (1 KB)  PDF (1208 KB)  ( 382 ) 28 The Relationship between Serum Leptin and Adiponectin Levels with Thyroid Hormone and Bone Metabolism in Patients with Hyperthyroidism ZHANG Tao, YU Yang, SUI Miao, et al DOI: 10.3969/j.issn.1006-6233.2020.01.007 Objective: To compare and analyze the changes of serum leptin and adiponectin levels in patients with hyperthyroidism and their relationships with thyroid hormone levels and bone metabolism. Methods: 78 patients with hyperthyroidism from April 2018 to March 2019 were selected as hyperthyroidism group, and 56 healthy volunteers were selected as control group. At the same time, patients with hyperthyroidism were divided into osteoporosis group (21 cases) and non-osteoporosis group (57 cases) according to whether they had osteoporosis. Serum leptin level was detected by radioimmunoassay and serum adiponectin level was detected by enzyme-linked immunosorbent assay (ELISA). The levels of serum thyroid hormone indexes such as triiodothyronine (T3), tetraiodothyronine (T4), free triiodothyronine (FT3), free thyroxine (FT4), parathyroid hormone (PTH), thyrotropin (TSH) were detected by chemiluminescence. The levels of bone metabolism-related cytokines such as interleukin-6 (IL-6), tumor necrosis factor (TNF), calcitonin (CT) and insulin-like growth factor-II (IGF-II) were measured by ELISA. The levels of osteocalcin (BGP) and bone specific alkaline phosphatase (BAP) were measured by radioimmunoassay. The levels of N-terminal osteocalcin (N-MID) and bone-derived alkaline phosphatase (ABAP) were measured by chemiluminescence. Bone mineral density (BMD) of femoral neck was measured. Pearson method was used to analyze serum leptin and adiponectin in patients with hyperthyroidism and their correlations with each index; the diagnostic value of serum leptin and adiponectin in hyperthyroidism-induced osteoporosis was analyzed by receiver operating characteristic curve (ROC). Results: The levels of serum leptin and adiponectin in hyperthyroidism group were significantly higher than those in control group (P < 0.05); the levels of T3, T4, FT3, FT4 and PTH in thyroid hormone indexes of hyperthyroidism group were significantly higher than those of control group (P < 0.05), and TSH level was significantly lower (P < 0.05); the levels of bone metabolism-related cytokines TNF, CT and IGF-II in hyperthyroidism group were significantly lower than those in control group (P < 0.05). The level of IL-6 was significantly higher than that of the control group (P < 0.05). The levels of ALP, BGP, BAP, N-MID and ABAP in hyperthyroidism group were significantly higher than those in control group (P < 0.05), while BMD in femoral neck was significantly lower (P < 0.05). Pearson analysis showed that serum leptin was positively correlated with adiponectin (P < 0.05), and positively correlated with T3, T4, FT3, FT4 and PTH (P < 0.05), negatively correlated with TSH (P < 0.05); Pearson analysis showed that leptin and adiponectin were positively correlated with ALP, BGP, BAP, N-MID, ABAP and IL-6 (P < 0.05), but negatively correlated with BMD, TNF, CT and IGF-II of femoral neck (P < 0.05). The serum leptin and adiponectin levels in osteoporosis group were significantly higher than those in non-osteoporosis group (P < 0.05). ROC analysis showed that the AUC of leptin in the diagnosis of osteoporosis caused by hyperthyroidism was 0.806, the sensitivity was 74.36%, the specificity was 76.79%, and the truncation value was 7.31ng/mL. The AUC of adiponectin in the diagnosis of osteoporosis caused by hyperthyroidism was 0.849, the sensitivity was 71.79%, the specificity was 85.71%, and the truncation value was 249.86 mg/dL. Conclusion: Serum leptin and adiponectin levels are abnormally increased in patients with hyperthyroidism. It is closely related to thyroid hormone level and bone metabolism index. It can also be used as an important index for early diagnosis of osteoporosis caused by hyperthyroidism. 2020 Vol. 26 (1): 28-32 [Abstract] ( 186 ) HTML (1 KB)  PDF (1409 KB)  ( 424 ) 33 Effects of Laparoscopy and Laparotomy on Immune Indexes and Cytokines in Children with Appendiceal Perforation Peritonitis and Sepsis WAN Mananjie, LI Chengchen DOI: 10.3969/j.issn.1006-6233.2020.01.008 Objective: To investigate the effects of laparoscopy and laparotomy on immune indexes and cytokines in children with appendiceal perforated peritonitis and sepsis. Methods: 64 children with appendicitis perforated peritonitis admitted to our hospital from March 2017 to June 2018 were enrolled. All subjects were divided into observation group and control group by simple random number table method, 32 cases in each group. Laparoscopic surgery was performed in the observation group, and traditional open surgery was performed in the control group. The changes of IgG, IgM, IL-6, IL-10 levels and postoperative complications were compared between the two groups before and after surgery. Results: Before operation, there was no significant difference in the levels of IgG, IgM, IL-6 and IL-10 between the two groups (P>0.05). On the 1st day after operation, the levels of IgG and IL-10 in the two groups were lower than those before operation, while the levels of IL-6 were higher than those before operation. The difference was statistically significant (P<0.05). There was no significant difference in the levels of IgM between the two groups before operation (P>0.05). On the 3rd day after operation, the levels of IgG and IL-6 in the two groups were lower than those of the 1st day after operation, while the levels of IL-10 were higher than those of the 1st day after operation. The difference was statistically significant (P<0.05). There was no significant difference between the levels of IgM and the 1st day after operation (P>0.05). There was no significant difference in postoperative complications between the two groups (P>0.05). The incidence of postoperative complications in the observation group was 6.25%, which was lower than that in the control group (25.00%), and the difference was statistically significant (P<0.05). Conclusions: Laparoscopy is superior to laparotomy in improving immune indexes and cytokine levels in children with appendical perforation peritonitis and sepsis, which is worthy of clinical promotion. 2020 Vol. 26 (1): 33-36 [Abstract] ( 128 ) HTML (1 KB)  PDF (1215 KB)  ( 273 ) 37 The Value of Serum OPG and TRAIL in Preoperative Evaluation of Lymphatic Metastasis of Esophageal Cancer TAN Zhigang, JIANG Guojun, DAI Ting, et al DOI: 10.3969/j.issn.1006-6233.2020.01.009 Objective: To investigate the relationship between serumosteoprotegerin (OPG), tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and lymphatic metastasis of esophageal cancer, and to explore its value in preoperative evaluation of lymphatic metastasis of esophageal cancer. Methods: 48 patients with esophageal cancer were selected as the study object, among which 24 patients with lymph node metastasis confirmed by postoperative pathology were the study group, and 24 patients without lymph node metastasis were the control group. The serum levels of OPG and trail were detected by ELISA and caspase protein was detected by Western blot. ROC curve was used to analyze serum OPG, trail and trail / OPG ratio for preoperative evaluation of esophageal cancer with or without lymphatic metastasis. Results: The serum OPG level in esophageal cancer patients with lymphatic metastasis was significantly higher than those without lymph node metastasis, whereas the serum TRAIL level and the ratio of TRAIL/OPG were significantly lower (P＜0.05). The best cut-off points of serum OPG and TRAIL for the diagnosis of esophageal cancer with lymphatic metastasis were ＞0.495pmoL/mL and＜0.485pmoL/mL, respectively, with the diagnostic sensitivity, specificity and accuracy of 91.7%, 37.5%, 64.6% and 75.0%,58.3%,67.0%, respectively. The sensitivity, specificity and accuracy of TRAIL/OPG ratio at the best cut-off point (＜0.693) for the diagnosis of esophageal cancer with lymphatic metastasis were 79.2%,83.3% and 81.3%, respectively. Conclusion: TRAIL-mediated apoptotic pathway may be related to lymphatic metastasis of esophageal cancer, thus TRAIL/OPG ratio can be a promising auxiliary clinical index for evaluating lymphatic metastasis of esophageal cancer before surgery. 2020 Vol. 26 (1): 37-40 [Abstract] ( 148 ) HTML (1 KB)  PDF (1219 KB)  ( 294 ) 41 Effect of Laparoscopic Cadical Gastrectomy for Distal Gastric Cancer of Billroth ⅡAnastomosis Combined with Braun Anastomosis on Intestinal Barrier Function and Serum Inflammatory Factors Levels in Gastric Cancer TIAN Qinglin, LI Yang, CHEN Xiaofeng DOI: 10.3969/j.issn.1006-6233.2020.01.010 Objective: To explore the effect of laparoscopic radical gastrectomy for distal gastric cancer of Billroth Ⅱanastomosis combined with Braun anastomosis on intestinal barrier function and serum inflammatory factors levels in gastric cancer. Methods: A retrospective analysis was performed on clinical data of each 56 patients who underwent laparoscopic radical gastrectomy for distal gastric cancer of Billroth Ⅱanastomosis (control group) and BillrothⅡanastomosis combined with Braun anastomosis (observation group) in our hospital. Perioperative indicators (operative time, gastrointestinal reconstruction time, intraoperative blood loss, postoperative exhaust time, postoperative hospital stay) and occurrence of postoperative complications were recorded in the two groups, and the intestinal barrier function [diamine oxidase (DAO), D-lactic acid], inflammatory factors [interleukin-6 (IL-6), interleukin-10 (IL-10)] and immune function [complements C3 , C4] were compared between the two groups before operation and at third day after operation. Results: There were no significant differences in the operative time, gastrointestinal reconstruction time, intraoperative blood loss, postoperative exhaust time and postoperative hospital stay between the two groups (P>0.05). The total incidence rate of postoperative complications in observation group was significantly lower than that in control group (P<0.05). At 3d after operation, the intestinal barrier function (serum DAO, D-lactic acid) and inflammatory factors (serum IL-6, IL-10) in the two groups were higher than those before operation (P<0.05), and the indexes in control group were higher than those in observation group (P<0.05). The immune function indexes (serum complements C3, C4) in the two groups were lower than those before operation (P<0.05), and the indexes in control group were lower than those in observation group (P<0.05). Conclusion: Billroth Ⅱanastomosis combined with Braun anastomosis has significant effects in laparoscopic radical gastrectomy for distal gastric cancer. And it can improve the postoperative intestinal barrier function and inflammatory level, and reduce the impact of surgery on immune function so as to help reduce postoperative complications. 2020 Vol. 26 (1): 41-44 [Abstract] ( 193 ) HTML (1 KB)  PDF (1200 KB)  ( 345 ) 45 Efficacy of Zoledronic Acid Combined with Calcium Carbonate D3 in the Treatment of Diabetic Osteoporosis and its Effects on Bone Mineral Density and Bone Metabolism Indexes REN Hua, ZHANG Zhiliang, CAI Wei, et al DOI: 10.3969/j.issn.1006-6233.2020.01.011 Objective: To investigate the efficacy of zoledronic acid combined with calcium carbonate D3 in the treatment of diabetic osteoporosis (DOP) and its effects on bone mineral density and bone metabolism indexes. Methods: 110 patients with DOP admitted to our hospital from September 2015 to May 2018 were selected and randomly group. 55 patients in control group were treated with Caltrate D3, and 55 patients in experimental group were given zoledronic acid combined with calcium carbonate D3. The clinical efficacy, bone mineral density, bone metabolism indexes, pain, incidence rate of complications and inflammatory factors were analyzed in the two groups. Results: After treatment, the total effective rate in experimental group (94.54%) was significantly higher than that in control group (80.00%) (P<0.05). After treatment, the bone mineral density values of lumbar vertebrae L1~4, femoral neck, femoral trochanter and Wards triangle region in the two groups were significantly increased compared with those before treatment (P<0.05), and the increases in experimental group were greater than those in control group (P<0.05). There were no significant differences in the levels of bone metabolism indexes of serum phosphorus and serum calcium between the two groups before and after treatment (P>0.05). After treatment, the levels of bone metabolism indexes of bone alkaline phosphatase (BALP), bone gla protein (BGP) and β-collagen fragment (β-CTX) and pain visual analogue scale (VAS) score in the two groups were decreased compared with those before treatment (P<0.05), and the decreases in experimental group were greater than those in control group (P<0.05). During treatment, there was not statistically significant difference between the two groups in the incidence rate of complications (P>0.05). After treatment, the levels of interleukin (IL-6), interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) in the two groups were significantly decreased compared with those before treatment, and the decreases in experimental group were greater than those in control group (P<0.05). Conclusion: Zoledronic acid combined with calcium carbonate D3 in the treatment of DOP can increase bone mineral density, inhibit bone resorption, promote bone formation and reduce body inflammation, and is safe and effective. 2020 Vol. 26 (1): 45-49 [Abstract] ( 151 ) HTML (1 KB)  PDF (1224 KB)  ( 358 ) 50 Correlation between Apolipoproteins B/A1 and Homocysteine with Elderly Coronary Heart Disease QIAN Jinhua, HU Xiaowei DOI: 10.3969/j.issn.1006-6233.2020.01.012 Objective: To investigate the relationship between the ratio of apolipoprotein B/A1 and the level of homocysteine and coronary heart disease (CAD) in the elderly. Methods: From January 2017 to June 2019, 81 patients in Huangshan people's hospital were selected for coronary angiography. 36 patients in group A were 30% < 70% of the degree of coronary stenosis, 45 patients in group B were ≥ 75% of the degree of coronary stenosis, and 30 patients in the control group were negative in the same period. The levels of serum Hcy, apoB, ApoA1, HDL-C and LDL-C were measured. The ratio of serum apoB / A1 and the relationship between serum Hcy and senile coronary heart disease were analyzed. Results: The levels of aopa1, apoB, apoB / A1, Hcy and LDL-C in group A and group B were significantly higher than those in the control group (P < 0.05), and the levels increased with the aggravation of patients' condition; the levels of HDL-C in group A and group B were significantly lower than those in the control group (P<0.05). ApoB / A1 were the risk factor of coronary artery stenosis (P<0.01). Conclusion: Apolipoprotein B/ A1 and homocysteine show a rising trend with the increase of the condition of patients with coronary heart disease, and the level of AopB/ A1 of great significance to the prediction of elderly patients with coronary heart disease. And is worthy of clinical use. 2020 Vol. 26 (1): 50-53 [Abstract] ( 133 ) HTML (1 KB)  PDF (1214 KB)  ( 304 ) 54 The Diagnostic Value of CRP Combined with PCT in Postoperative Pulmonary Infection of Elderly Patients with Hip Fracture NI Yu DOI: 10.3969/j.issn.1006-6233.2020.01.013 Objective: To investigate the changes of serum CRP and PCT levels in elderly patients with hip fracture and their diagnostic value in postoperative pulmonary infection. Method: 80 elderly patients with hip fracture treated with surgery were selected as the objects of observation. According to the occurrence of pulmonary infection, they were divided into the infection group (n=40) and the non-infection group (n=40).Immune turbidimetric method, chemiluminescence method were used respectively to measure CRP and PCT levels,before surgery, 12 hours after operation, postoperative 4 7 days, the change trend of the two kinds of indexes were observed, and evaluate the CRP and PCT specific degrees, the sensitivity of diagnosis of lung infection. Results: 12 hours after the operation, the CRP level in the infected group and the non-infected group was higher than that before the operation, and P<0.05.4 7 days postoperative infection group, CRP levels is increased after 12 hours, and P<0.05. 12 hours after the operation, the PCT level of the infected group and the non-infected group was significantly higher than that before the operation, and P<0.05. PCT level in the infection group 4 7 days after surgery was higher than that 12 hours after surgery, and P < 0.05.ROC curve analysis showed that the AUC values of the two detection indexes were 0.8238 and 0.9156 at the optimal boundary point, respectively. Both CRP and PCT had good accuracy in diagnosis. The diagnostic sensitivity of PCT was significantly higher than that of CRP, and the diagnostic specificity of CRP was slightly higher than that of PCT. Conclusion: CRP and PCT have high application value in the early diagnosis of elderly patients with postoperative hip fracture complicated by pulmonary infection. 2020 Vol. 26 (1): 54-57 [Abstract] ( 142 ) HTML (1 KB)  PDF (1271 KB)  ( 287 ) 58 Study on Lnc RNA PCGEM1 Regulating the Invasion and Metastasis of Esophageal Cancer Cells through the TGF beta/Smad Signaling Pathway LIU Yong, WANG Bin, JIN Jianqin, et al DOI: 10.3969/j.issn.1006-6233.2020.01.014 Objective: To investigate the regulation of long-chain noncoding RNA PCGEM1(Lnc RNA PCGEM1)in the invasion and metastasis of esophageal cancer cells through the TGF β2/Smad2 signaling pathway. Methods: Cancer tissues and adjacent normal tissues of 62 EC patients were collected. QRT-pPCR was used to detect the expression of Lnc RNA PCGEM1 in EC and corresponding paracancer tissues and different cells. Lnc RNA PCGEM1 silenced cell lines were constructed, and the cells were divided into Eca-109-siPCGEM1 group, negative control Eca-109-siNC group, and Eca-109 as blank control group.MiR-148a silenced cell lines of Eca-109-siPCGEM1 cells were constructed, which were divided into siPCGEM1+ simiR-148a group and siPCGEM1+siNC group.Eca-109 cell proliferation was detected by plate cloning assay.The effect of Eca-109 cell migration was detected by scratch assay. The bioinformatics website StarBase was used to predict the complementary binding microRNA (miRNA) of PCGEM1, and the targeted binding genes of corresponding miRNA were predicted based on the Targetscan website. Western Blot analysis of TGF β2/Smad2 signaling pathway protein expression. Results: QRT-PCR results showed that the expression level of Lnc RNA PCGEM1 in EC tissues and esophageal cancer cell lines was higher than that in adjacent normal tissues (P<0.05). Compared with other cell lines, Lnc RNA PCGEM1 had the highest expression in Eca-109 cells (P<0.05). Compared with the blank control group, the number of cell clones and migration in the Eca-109-siPCGEM1 group was significantly reduced, and the expression of miR-148a was significantly increased (P<0.05)..There was no significant difference between the blank control group and Eca-109-siNC group (P>0.05).Compared with the siPCGEM1+siNC group, the number of cell clones, number of migration were significantly increased in the siPCGEM1+ simiR-148a group (P<0.05). The prediction results of StarBase website showed that Lnc RNA PCGEM1 could be complementary to miR-148a, and the prediction analysis of Targetscan website showed that miR-148a had a targeted binding site with TGF β2.Western blot results showed that the expressions of TGF β2 and Smad 2 in Eca-109-siPCGEM1 group were significantly lower than those in blank control group and Eca-109-siNC group (P<0.05).The expression levels of the above indicators in the blank control group and Eca-109-siNC group were not statistically significant (P>0.05). Conclusion: Lnc RNA PCGEM1 is highly expressed in esophageal cancer. High expression of Lnc RNA PCGEM1 may enhance the TGF β2/Smad2 signaling pathway by downregulation of miR-148a, thus promoting the development of EC. 2020 Vol. 26 (1): 58-62 [Abstract] ( 141 ) HTML (1 KB)  PDF (2165 KB)  ( 388 ) 63 Effects of Neoadjuvant Chemotherapy and ISR on Anorectal Dynamics and Defecation in Patients with Rectal Cancer LIU Yang, LI Dapeng, GAO Fei, et al DOI: 10.3969/j.issn.1006-6233.2020.01.015 Objective: To explore the effects of neoadjuvant chemotherapy and ISR on anorectal dynamics and defecation in patients with rectal cancer. Methods: 73 rectal cancer patients who underwent ISR in our hospital from January 2015 to November 2016 were selected as the study objects. According to the treatment methods, they were divided into two groups: the control group (35 cases) received conventional ISR, and the observation group (38 cases) received neoadjuvant chemotherapy combined with ISR. The anorectal motility, defecation ability and adverse reactions were compared between the two groups. Results: In the two groups, the changes of hpz, ARP and MSP were significant (P < 0.05) compared with each other at different time points, and the changes of ARP and MSP in the control group were significantly higher than those in the control group at 1 month, 3 months and 6 months after operation (P < 0.05). There was no significant difference between the two groups (P > 0.05). Infection, intestinal obstruction, urinary retention and other events occurred in the 2 groups * but there was no significant difference in the incidence of adverse events between the 2 groups (P>0.05). Conclusion: Neoadjuvant chemotherapy combined with ISR in the treatment of rectal cancer has certain influence on anorectal motility, but it does not increase defecation difficulties and other adverse events. 2020 Vol. 26 (1): 63-66 [Abstract] ( 110 ) HTML (1 KB)  PDF (1214 KB)  ( 287 ) 67 Efficacy of CRRT on Severe Pancreatitis and Its Influence on Pancreatic Microcirculation Indexes and Serum Ghrelin and PCT Levels LIU Yan, CHEN Qin, YUAN Xue, et al DOI: 10.3969/j.issn.1006-6233.2020.01.016 Objective: To analyze the efficacy of continuous renal replacement therapy (CRRT) in the treatment of severe pancreatitis and its influence on pancreatic microcirculation indexes and serum Ghrelin and procalcitonin (PCT) levels. Methods: The clinical data of each 102 patients with severe pancreatitis treated by CRRT (observation group) and conventional treatment (control group) were retrospectively analyzed. The times of clinical indexes remission were recorded in the two groups, and pancreatic microcirculation indexes [pancreatic blood flow (BF), blood flow volume (BV)], serological markers [Ghrelin, PCT, thromboxane A2 (TXA2)] and acute physiology and chronic health evaluation (APACHE II), and Balthazar computed tomography (CT) severity index (CTSI) were compared between the two groups before treatment and after 3d of treatment. Results: The recovery time of serum amylase, bowel sounds, abdominal pain remission and abdominal distension remission in observation group were lower than those in control group (P<0.05). After 3d of treatment, the pancreatic microcirculation indexes (pancreatic BF, BV) in the two groups were increased compared with those before treatment (P<0.05), and the indexes in observation group were higher than those in control group (P<0.05). Serological markers (serum Ghrelin, The PCT, TXA2) and scores of APACHE II and CTSI in the two groups were decreased compared with those before treatment (P<0.05), and the markers and scores in observation group were lower than those in control group (P<0.05). Conclusions: CRRT therapy has significant effects in the treatment of severe pancreatitis. And it can not only promote the relief of symptoms, but also relieve the pancreatic microcirculation disorder, and it has positive significance in disease outcomes of patients. 2020 Vol. 26 (1): 67-70 [Abstract] ( 147 ) HTML (1 KB)  PDF (1211 KB)  ( 360 ) 71 Efficacy of Terbutaline Combined with Glucocorticoid and Glutathione in the treatment of Acute Exacerbation of Chronic Obstructive Pulmonary Disease and Its Effects on Inflammatory Factors Levels LIU Xiaodong, HU Ke DOI: 10.3969/j.issn.1006-6233.2020.01.017 Objective: To study the efficacy of terbutaline combined with glucocorticoid and glutathione in the treatment of acute exacerbation of chronic obstructive pulmonary disease (AECOPD) and its effects on inflammatory factors. Methods: 150 patients with AECOPD admitted to the hospital from December 2017 to December 2018 were included in the study. According to the clinical drug regimens, they were divided into group A (tebutaline, n=35) and group B (tebutaline +glucocorticoid, n=61) and group C (tebutaline + glucocorticoid + glutathione, n=54). The treatment effects were observed in the three groups. Results: There was a significant difference in the effective rate of treatment among the three groups (P<0.05), and the effective rate of treatment was the highest in group C, followed by group B, the lowest in group A. After treatment, the scores of cough, expectoration and shortness of breath in groups A, B and C showed downward trends in turn (P<0.05). After treatment, the levels of forced vital capacity (FVC), forced expiratory volume in 1 second (FEV1) and FEV1/FVC showed upward trend in turn in group A, group B and group C (P<0.05). After treatment, the levels of serum C-reactive protein (CRP) and tumor necrosis factor-α (TNF-α) showed downward trends in groups A, B and C (P<0.05). There were no significant differences in the adverse drug reactions among the three groups (P>0.05). Conclusion: Terbutaline combined with glucocorticoid and glutathione can effectively improve lung function, relieve clinical symptoms, reduce the body inflammatory factors, and improve effective rate of treatment in patients with AECOPD, and it has few adverse drug reactions. 2020 Vol. 26 (1): 71-75 [Abstract] ( 98 ) HTML (1 KB)  PDF (1192 KB)  ( 393 ) 76 Inhibiting MicroRNA-27b Regulation of Nrf2/ARE Signaling Pathway on Brain Injury in Rat Model of Hypertensive Cerebral Hemorrhage LIU Xiaojiang, LI Jun, GUAN Yixiang DOI: 10.3969/j.issn.1006-6233.2020.01.018 Objective: To investigate the effect of microrna-27b (mir-27b) inhibition on brain injury in hypertensive cerebral hemorrhage (HICH) rats, and to detect the expression of Nrf2 / are signal pathway and its downstream protein through the intervention of mir-27b antagonist, and to explore the therapeutic mechanism of mir-27b in anti-oxidation and neuroinflammation in hypertensive cerebral hemorrhage. Methods: The model of hypertensive intracerebral hemorrhage was established by injecting blood into the brain. QRT-PCR was used to detect the expression of mir-27b and Nrf2 mRNA in the brain of rats with hypertensive intracerebral hemorrhage; Western blot was used to detect the expression of Nrf2 / are signal pathway protein nuclear factor-related factor-2 (Nrf2), heme oxygenase (HO-1) and quinone oxidoreductase (NQO1) before and after administration of mir-27b antagonist; the kit was used to detect the superoxide dismutation of rats with hypertensive intracerebral hemorrhage before and after administration The changes of SOD, TNF - α and IL-1 β in rats with hypertensive cerebral hemorrhage were detected by TUNEL and flow cytometry. Results: Mir-27b and Nrf2 mRNA levels were significantly different before and after hich (P < 0.05); mir-27b antagonists could induce Nrf2, HO-1 and NQO1 protein expression in Nrf2 / are pathway; mir-27b antagonists could alleviate oxidative stress response (OS) in hich rats The mir-27b antagonist can alleviate the apoptosis of nerve cells and reduce the brain injury. Conclusion: Inhibition of mir-27b expression can significantly reduce the degree of brain injury in rats with hypertensive intracerebral hemorrhage, and its mechanism may be related to the activation of Nrf2 / are. 2020 Vol. 26 (1): 76-79 [Abstract] ( 153 ) HTML (1 KB)  PDF (1504 KB)  ( 302 ) 80 Meta-analysis of the Relationship between the Expression Level of GINS and the Prognosis of Tumor Patients WANG Shibo, et al DOI: 10.3969/j.issn.1006-6233.2020.01.019 Objective: To evaluate the relationship between GINS expression levels and the prognosis of tumor patients used by meta-analysis. Methods: The articles about the relationship between the expression level of GINS and the prognosis of cancer patients in Wanfang medical network, VIP database and PubMed database were retrieved. The retrieval time was up to April 30, 2019, and the meta-analysis was carried out with Revman 5.3 software. Results: 7 articles including 722 patients included in the study about the relationship between GINS gene expression levels and overall survival (OS), the results showed high GINS gene expression of tumor patients significantly shortened OS (HR = 2.11, 95% CI 1.43 ~ 3.10, P = 0.0002);3 articles including 991 patients included in the study about the relationship between GINS gene expression levels and disease-free survival (DFS), the results showed high GINS gene expression of tumor patients significantly shortened DFS (HR = 1.60, 95% CI 1.38 ~ 1.86, P = 0.00001). Conclusion: The high expression of GINS gene has a certain relationship with the poor prognosis of tumor patients, suggesting that GINS may be a potential target for tumor prognosis. 2020 Vol. 26 (1): 80-83 [Abstract] ( 239 ) HTML (1 KB)  PDF (1534 KB)  ( 321 ) 84 Serum Levels of HIF-1α and VEGF in Patients with Craniocerebral Injury and Their Relationship with Severity Degree and Prognosis DAI Ling, et al DOI: 10.3969/j.issn.1006-6233.2020.01.020 Objective: To investigate the serum levels of HIF-1α and VEGF in patients with craniocerebral injury and their relationship with severity degree and prognosis. Methods: 198 patients with craniocerebral injury (study group) and 70 cases of health examinees (control group) in Panzhihua Central Hospital from February 2017 to December 2018 were selected. The study group was re-divided into four subgroups according to the Glasgow Coma Scale (GCS) and post-treatment daily living ability, namely mild group (n=62), moderate group (n=76), severe group (n=60), good prognosis group (n=140) and poor prognosis group (n=58). The levels of HIF-1α and VEGF were detected by enzyme-linked immunosorbent assay (ELISA) on the 1st, 3rd and 7th day after admission, and their relationship with prognosis were compared. Results: The serum levels of HIF-1a and VEGF in the study group were significantly higher than those in the control group (P<0.05). The levels of serum HIF-1α and VEGF on the 1st, 3rd and 7th day after admission ranking in a descending order severe group, moderate group and mild group (P<0.001). The serum levels of HIF-1α and VEGF in the poor prognosis group were significantly higher than those in the prognosis group (P<0.001). Logistic regression analysis showed moderate degree (OR=3.39, 95%CI: 2.66~4.32), severe degree (OR=5.19, 95%CI: 3.89~6.92), serum HIF-1α≥62.24ng/ml (OR=3.48, 95%CI: 2.47~4.90), and VEGF≥160.91pg/ml (OR=4.67, 95%CI: 2.81~7.76) were risk factors for good prognosis in patients with craniocerebral injury. Conclusion: The levels of serum HIF-1 α and VEGF increased in patients with brain injury, and even higher in patients with poor prognosis. Therefore, the levels of serum HIF-1 α and VEGF have an important relationship with the prognosis of patients with brain injury. 2020 Vol. 26 (1): 84-87 [Abstract] ( 123 ) HTML (1 KB)  PDF (1205 KB)  ( 491 ) 88 MiR-23a Promoting Cell Proliferation of Human Tongue Squamous Cell Carcinoma Cell through Regulating PPP2R5E TAO Yadong, et al DOI: 10.3969/j.issn.1006-6233.2020.01.021 Objective: To investigate the effect mechanism of miR-23a on the proliferation of human tongue squamous cell carcinoma cells. Methods: Clinical tissue samples of tongue squamous cell carcinoma were collected and Tca8113 and CAL27 were cultured. Real-time quantitative PCR was performed to detect the expressions of miR-23a and PPP2R5E in Clinical tissue samples of tongue squamous cell carcinoma. MTT assay, colony formation assay and growth curve assay were used to detect the effect of miR-23a and PPP2R5E on the proliferation of human tongue squamous carcinoma cell. Luciferase reporter assay verified the regulatory relationship between mir-23a and PPP2R5E. Results: The expression of miR-23a in tongue squamous cell carcinoma was significantly up-regulated(P<0.01). MiR-23a promoted the proliferation of tongue squamous cell carcinoma cells. Bioinformatics prediction and luciferin reporting experiments showed that PPP2R5E was a direct target gene of miR-23a. The expression of PPP2R5E was decreased in tongue squamous cell carcinoma. PPP2R5E inhibited the proliferation of tongue squamous cell carcinoma cells. Overexpression of PPP2R5E can reverse the proliferation promoting effect of mir-23a on tongue squamous cell carcinoma cells. Conclusion: MiR-23a can promote the proliferation of tongue squamous cell carcinoma cells through PPP2R5E. MiR-23a plays an oncogene role in the occurrence and development of tongue squamous cell carcinoma. 2020 Vol. 26 (1): 88-92 [Abstract] ( 152 ) HTML (1 KB)  PDF (1990 KB)  ( 400 ) 93 Effect of Anticoagulant Therapy on Serum TNF-α and Interleukin Expression in Patients with Chronic Obstructive Pulmonary Disease CHEN Qingyun, LI Jiwei, LIAO Dongchen, et al DOI: 10.3969/j.issn.1006-6233.2020.01.022 Objective: To study effect of TNF-α and interleukin(IL-18andIL-6)in patients with chronic obstructive pulmonary disease using anticoagulant drugs. Method: 94 patients were divided into two groups, 47 in each group. The conventional group used common therapy, and the anticoagulation group used anticoagulant drugs on the basis of the conventional group. Observe the following indicator changes. Results: The effective rate of COPD patients in the anticoagulation group was significantly higher than that in the conventional group(P＜0.05). There was no difference between the two groups before treatment (P > 0.05). After treatment, the levels of TNF - α, IL-18 and IL-6 in the two groups were significantly lower than those in the anticoagulant group (P < 0.05). The patients in the routine group and anticoagulant group had different degrees of ecchymosis, increased platelet count and other symptoms. The single ecchymosis disappeared by itself, and the platelet and coagulation time returned to the normal level. There was no difference between the two groups (P > 0.05). Conclusion: The expression of TNF - α, IL-18 and IL-6 in the serum of patients with COPD can be significantly inhibited by antitussive and expectorant drugs combined with anticoagulant therapy. The clinical effect of COPD is obviously improved, which is worth popularizing. 2020 Vol. 26 (1): 93-96 [Abstract] ( 151 ) HTML (1 KB)  PDF (1207 KB)  ( 294 ) 97 Effect of Nalmefene Hydrochloride Combined with Mouse Nerve Growth Factor in Newborns with Hypoxic Ischemic Encephalopathy and Its Influence on Background Electroencephalogram Activity and Prognosis DING Lili, WANG Jun DOI: 10.3969/j.issn.1006-6233.2020.01.023 Objective: To explore the effect of Nalmefene hydrochloride combined with mouse nerve growth factor (mNGF) in newborns with hypoxic ischemic encephalopathy (HIE), and to analyze on its influence on background electroencephalogram activity and prognosis. Methods: 104 cases of neonatal HIE were selected as subjects and divided into routine group and combined group by simple randomization method. The routine group was given basic support and mNGF therapy. The combined group was given basic support + mNGF + Nalmefene hydrochloride. Background electroencephalogram activity changes before and after treatment, clinical effect, drug adverse reactions and sequel were compared. Results: There was no significant difference in baseline data between the two groups (P> 0.05). After treatment, the incidences of resting, burst-suppression, low voltage, diffuse americium wave and single-rhythm discharges in the two groups decreased significantly (P< 0.05). After treatment, the incidences of abnormal electroencephalogram activity in the combined group were significantly lower than those in the routine group (P< 0.05). The total effective rate was 88.00% in the combined group, which was significantly higher than that of 70.00% in the routine group (P< 0.05). There was no significant difference in the incidence of every drug adverse reaction and total drug adverse reactions between the two groups (P> 0.05). The incidences of epilepsy and total poor prognosis in the combined group were 4.00% and 4.00%, respectively, which were significantly lower than those of 14.00% and 16.00% in the routine group (P< 0.05). Conclusion: Nalmefene hydrochloride combined with mNGF for neonatal HIE can significantly improve electroencephalogram activity, achieve ideal effect, safety and reliability, and effectively reduce adverse prognosis. 2020 Vol. 26 (1): 97-100 [Abstract] ( 130 ) HTML (1 KB)  PDF (1214 KB)  ( 333 ) 101 Effects of Metformin Combined with Ethinyl Estradiol Cyproterone on Polycystic Ovary Syndrome HOU Jing, ZHANG Yi, GUO Yanwei, et al DOI: 10.3969/j.issn.1006-6233.2020.01.024 Objective: To investigate the effect of Metformin combined with Ethinyl Estradiol Cyproterone in the treatment of polycystic ovary syndrome (PCOS). Methods: 90 PCOS cases in our hospital from January 2017 to January 2018 were randomly divided into control group (oral Ethinylestradiol Cycloprogesterone) and observation group (oral Ethinylestradiol Cycloprogesterone + Metformin) by the random number table with simple randomization method, 45 cases in each group, corresponding treatment was given. The clinical data of the two groups were recorded .Serum galactose lectin - 3 (Galectin - 3) level, sex hormones, blood lipid, fasting blood sugar, insulin levels and other indicators of the patients were measured before and after treatment. Results: Before treatment, there was no significant difference between the observation group and the control group (P>0.05). After treatment, the difference before and after treatmentthe of body mass index (BMI), waist-to-hip ratio (WHR), Galectin-3 level, luteinizing hormone (LH), testosterone (T), estradiol (E2), triglyceride (TG), high-density lipoprotein (HDL),low-density lipoprotein (LDL) and fasting serum glucose (FPG), fasting insulin (FINS), homeostasis model assessment-insulin resistance index (HOMA-IR) and ovulation rate and pregnancy rate were significantly higher than those of the control group, abortion rate was significantly lower than those of the control group, and the difference were statistically significant (P<0.05). There were no significant differences in the difference between follicle stimulating hormone (FSH) and total cholesterol (TC) in the observation group and the control group (P>0.05). Conclusion: Metformin combined with Ethinyl Estradiol Cyproterone has a good therapeutic effect on PCOS,which can effectively regulate sex hormone levels and alleviate insulin resistance. 2020 Vol. 26 (1): 101-104 [Abstract] ( 115 ) HTML (1 KB)  PDF (1224 KB)  ( 270 ) 105 Effect of Mechanical Nickel-titanium Instrument on Stress Reaction and Therapeutic Effect in Children with Deciduous Dental Pulp Disease Treated with Root Canal ZHANG Lei DOI: 10.3969/j.issn.1006-6233.2020.01.025 Objective: To analyze the effect of nitinol instrument on endodontic treatment of children with pulpopathy of primary teeth, and summarize the clinical experience. Methods: From May 2017 to May 2019, 100 children with primary dental pulp disease in our hospital were selected for comparative experiment, and randomly divided into control group and test group with 50 cases each by random number method. Children in the control group were prepared with conventional hand nitinol instruments, while children in the test group were prepared with mechanical nitinol instruments. The time of root canal preparation, the rate of good preparation effect and complications were recorded and analyzed. Results: The operation time of root canal preparation (62.1, 6.27, s) in the control group was significantly higher than that of the test group (45.18, 4.29, s), and there was a statistical difference (χ2=10.698,P＜0.001), and the number of children with good preparation effect in the control group (38 cases) was significantly lower than that of the test group (49 cases). In the control group, the good rate (76%) was lower than that of the control group (98%)(χ2=10.698,P＜0.001), and the root canal obstruction (3,6%), lateral shift (1,2%) and the deformation (1,2%) of the children in the control group were all occurred. The overall incidence of complications (10%) was slightly lower Control (2%)(χ2=2.837,P=0.092). Conclusion: In the root canal treatment of children with pulpopathy of deciduous teeth, the use of mechanical nitinol instruments for root canal preparation can greatly reduce the operation time of root canal preparation, improve the preparation effect. For a certain extent, it can improve the postoperative recovery, which is worth promoting in the relevant clinical treatment. 2020 Vol. 26 (1): 105-108 [Abstract] ( 118 ) HTML (1 KB)  PDF (1200 KB)  ( 448 ) 109 Analysis of Pathogenic Microorganism, Drug Resistance and Factors in Children with Acute Attack of Bronchial Asthma Complicated with HAP ZHANG Chuanling, FANG Daihua, TIAN lijun DOI: 10.3969/j.issn.1006-6233.2020.01.026 Objective: To investigate the pathogenic microorganisms and drug resistance in children with acute attack of bronchial asthma complicated with hospital acquired pneumonia (HAP), and to analyze its risk factors. Methods: The clinical data of 609 cases of acute attack of bronchial asthma in children were retrospectively analyzed in our hospital from January 2016 to December 2018, and they were divided into HAP group and non-HAP group according to whether HAP occurred or not. The pathogenic microorganisms detection and drug susceptibility test were carried out of the HAP group, and the distribution characteristics and drug resistance were analyzed according to the test results, also the risk factors of of HAP in children with acute attack of bronchial asthma were analyzed by single factor analysis and multiple factor Logistic regression analysis method. Results: There were 30 cases with HAP among 609 children with acute attack of bronchial asthma, and the incidence rate of HAP was 4.93% (30/609). A total of 39 pathogens were detected in the HAP group, including 32 Gram-negative bacteria (82.50%), 4 Gram-positive bacteria (10.26%), 1 fungus (2.56%), 1 Mycoplasma (2.56%) and 1 Chlamydia (2.56%), and there was no virus or tuberculosis infection. The main Gram-negative bacteria in HAP pathogenic bacteria were highly resistant to ampicillin cefazolin and ceftriaxone (>35.00%), but the resistance rates to piperacillin-tazobactam, amikacin and imipenem were low (<15.00%). The main Gram-positive bacteria in HAP pathogenic bacteria showed high resistance to penicillin, oxacillin, erythromycin and clindamycin (>45.00%), but low resistance to gentamicin, vancomycin, levofloxacin, compound sulfamethoxazole, ciprofloxacin and rifampicin (<10.00%). Logistic regression analysis showed that invasive operation of respiratory system, preventive using of antibiotics, glucocorticoid using time >14d and hospitalization time >7d were independent risk factors for HAP in children with acute attack of bronchial asthma (OR = 3.655, 2.740, 2.596, 2.542, P < 0.05). Conclusion: Gram-negative bacteria are the main pathogens of HAP in children with acute attack of bronchial asthma, following by Gram-positive bacteria, and the main Gram-negative bacteria are lowly resistant to piperacillin-tazobactam, amikacin and imipenem, and the main Gram-positive bacteria are lowly resistant to gentamicin, vancomycin, levofloxacin, compound sulfamethoxazole, ciprofloxacin and rifampicin, in addition, invasive operation of respiratory system, preventive using of antibiotics, glucocorticoid using time and hospital stay too long can increase the risk of HAP, and it is necessary to use antibiotics reasonably in clinical work and take active intervention measures to reduce the occurrence of HAP. 2020 Vol. 26 (1): 109-113 [Abstract] ( 130 ) HTML (1 KB)  PDF (1214 KB)  ( 306 ) 114 Clinical Significance of Quantitative CT Measurement of Abdominal Fat Distribution in the Guidance of Total Surgery of Rectal Cancer WEI Jun, JIA Ruijuan, QIU Lei, et al DOI: 10.3969/j.issn.1006-6233.2020.01.027 Objective: To explore the clinical significance of quantitative CT measurement of abdominal fat distribution in the laparoscopic of total surgery of rectal cancer. Methods: The clinical data of 106 patients with rectal cancer admitted to our hospital from Jan. 2018 to Jan. 2019 were retrospectively analyzed. All patients were given laparoscopic radical resection of rectal cancer and given CT examination before surgery. According to the CT quantitative measurement results, patients with visceral fat area (VFA) > 100 cm2 were defined as VFA-L group (54 cases), and patients with VFA≦100 cm2 were defined as VFA-S group (52 cases). The intraoperative indicators (intraoperative blood loss, incision length, operative time, number of cases of conversion to laparotomy, lymph node dissection quantity) and postoperative indicators (postoperative feeding time, postoperative exhaust time, oxygen inhalation time, postoperative hospital stay, incidence rate of complications) indicators and mesangial integrity were compared between the two groups. Results: There were no significant differences in incision length, postoperative feeding time, postoperative exhaust time and oxygen inhalation time between the two groups (P>0.05), and the intraoperative blood loss in VFA-L group was greater than that in VFA-S group, and the operative time and postoperative hospital stay were longer than those in VFA-S group, and the lymph node dissection quantity was less than that in VFA-S group, and the number of cases of conversion to laparotomy was higher than that in VFA-S group, and the incidence rate of postoperative complications was higher than that in VFA-S group, and the mesangial integrity rate was lower than that in VFA-S group (P<0.05). Conclusions: High VFA in patients will increase the operation difficulty and risk of laparoscopic radical resection of rectal cancer, increase the possibility of postoperative complications and prolong hospital stay. Quantitative CT measurement of abdominal fat distribution can evaluate the operation difficulty and risk and provide a basis for surgical procedures and preoperative preparation. 2020 Vol. 26 (1): 114-117 [Abstract] ( 158 ) HTML (1 KB)  PDF (1213 KB)  ( 385 ) 118 Experience in the Treatment of Congenital Tenosynovitis due to Thumb Stenosis in Children SONG Defu, MA Yulong, WANG Xiaowei, et al DOI: 10.3969/j.issn.1006-6233.2020.01.028 Objective: To investigate the effect of mini-incision technique for congenital trigger thumb release. Methods: The clinical data of 30 children with congenital trigger thumb treated in our hospital from July 2016 to July 2018 were retrospectively analyzed. Patients were randomly divided into control group (n=39) and study group (n=39) according to different surgical treatment methods. The control group was treated with conventional surgical methods, and the study group received the mini-incision technique. Then the clinical efficacy, operation time, recovery time of affected finger function, hospital stay, complications, visual analogue scale (VAS) score and joint activity were compared between the two groups. Results: The total effective rate of the study group was significantly higher than that of the control group (P<0.05), and the operation time, the recovery time of the affected finger function and the hospitalization time of the study group were significantly shorter than those of the control group (P<0.05). VAS score and joint activity between the two groups showed no difference before treatment (P>0.05). After treatment, the VAS score was decreased, meanwhile the joint activity was increased in both groups after treatment, and the observation group had lower VAS score as well as better joint activity than those of the control group (P<0.05). The incidence rate of the complications in the study group was significantly lower than that in the control group (P<0.05). Conclusion: Mini-incision technique for congenital trigger thumb release has the advantages of simple operation, small wound, quick recovery (immediate intraoperative recovery of the affected finger extension and flexion function), definite efficacy, etc. It is a safe and reliable method for the treatment of children with congenital trigger thumb, which is worthy of clinical promotion and application. 2020 Vol. 26 (1): 118-121 [Abstract] ( 149 ) HTML (1 KB)  PDF (1213 KB)  ( 278 ) 122 Correlation Analysis of Immune Function, Microecological Changes and Correlation in Children with Wheezing Bronchitis REN Hua, ZHONG Hongping, LI Baocun, et al DOI: 10.3969/j.issn.1006-6233.2020.01.029 Objectives: To study the relationship between autoimmune function and microecological changes in children with wheezing bronchitis. Methods: 140 children with wheezing bronchitis admitted to our hospital from July 2015 to April 2018 were selected as the object of this study, as the observation group and 125 children with normal physical examination in our hospital as the control group. The indexes of autoimmune function and microecology were observed in the two groups. The correlation analysis was used to analyze the correlation between immune function and microecological indexes. Results: The levels of CD3+, CD4+, CD4+/CD8+, NK and interferon gamma in the observation group were as follows: (52.57±2.05)%, (23.62±0.89)%, (1.25±0.2)%, (12.88±0.36%)%, (44.02±1.51)ng/L, It was significantly lower than the control group (67.86±2.89)%, (41.53±1.37)%, (1.86±0.26)%, (17.51±0.22)%, (58.2±1.72)ng/L. The difference was significant (P<0.05). The IL-4, IL-10 indicators are: (60.49±3.53)ng/L, (14.13±1.18)ng/L, It was significantly higher than that of the control group (28.74±1.73)ng/L, (5.89±0.92)ng/L. The difference was significant (P<0.05). In the observation group, Bifidobacterium and Lactobacillus were as follows: (4.33±0.97)lg CFU, (5.07±0.60)lg CFU. It was significantly lower than the control group. (9.33±0.76)lg CFU, (9.56±0.65)lg CFU, Escherichia coli and staphylococci are Escherichia coli and Staphylococcus, respectively: (7.06±0.49)lg CFU, (4.11±0.82)lg CFU, It was significantly higher than that of the control group.(4.87±0.68)lg CFU, (1.74±0.42)lg CFU. The difference was significant (P<0.05). The indexes of CD3, CD4 and NK, interferon gamma were positively correlated with Bifidobacillus and Lactobacillus, negatively correlated with Escherichia coli and Staphylococcus, and negatively correlated with Bifidobacillus and Lactobacillus, and positively correlated with Escherichia coli and Staphylococcus. Conclusion: The imbalance of immune function and intestinal microecological flora in children with wheezing bronchitis may affect each other and the whole process of the disease. 2020 Vol. 26 (1): 122-125 [Abstract] ( 154 ) HTML (1 KB)  PDF (1211 KB)  ( 276 ) 126 Clinical Characteristics of Children with MPP Increased by D-dimer and Effect of Low Molecular Weight Heparin on Adjuvant Therapy LUO Jiaojiao, et al DOI: 10.3969/j.issn.1006-6233.2020.01.030 Objectives: To analyze the clinical characteristics of children with elevated D-dimer and the effect of low molecular weight heparin (LMWH) in children with MPP. Methods: 99 hospitalized children with Mycoplasma pneumoniae pneumonia with D-dimer elevation from May 2014 to may 2019 were randomly divided into three groups: group A, group B and group C. all the children in the three groups were given anti infection and routine symptomatic treatment. On this basis, group a combined with 100iu / kg LMWH for subcutaneous injection, and group B combined with 50iu / kg LMWH for basic treatment Group C maintained routine and anti infection treatment. Analysis of three groups of children before and after treatment chest imaging results and clinical symptoms, compared with the cost of hospitalization and hospitalization days. In addition, 33 cases of MPP without abnormal D-dimer in the same period of hospitalization were selected as the control group, and ESR and CRP and other inflammatory indexes, chest imaging results, clinical symptoms and hospitalization costs were compared between group C and control group. Results: In the control group, the inflammatory indicators such as ESR and CRP were significantly lower than those in the group C, and the hospital cost, length of stay, duration of fever reduction and cough remission of the former were significantly lower than those in the group C (all P<0.05). The majority of chest imaging findings in the control group were small films, while the majority of chest imaging findings in the group C were large films. The difference between the two groups was statistically significant (P<0.05). The hospitalization cost, length of stay, chest imaging improvement time and cough relief time of children in group a and group b were significantly lower than those in group C (all P<0.05). There was no significant difference in antipyretic time between groups A, B and C (P>0.05). In this study, all the children in each group had normal electrolyte and liver and kidney functions, and no adverse reactions such as bleeding and coagulation mechanism disorder occurred. Conclusion: In children with MPP, the symptoms and inflammatory indexes of children with abnormal D-dimer and chest imaging examination are more serious than those of normal children. Low molecular weight heparin can effectively promote the absorption of pulmonary lesions in children with MPP with elevated D-dimer, shorten the hospitalization time and reduce the cost of children, and will not increase the incidence of adverse reactions in a short period of time. 2020 Vol. 26 (1): 126-129 [Abstract] ( 148 ) HTML (1 KB)  PDF (1196 KB)  ( 452 ) 130 Study on Effect of Butylphthalide Capsule on Vascular Dementia after Stroke LIN Zhecong, HAN Qiaolin, WU Xiyan, et al DOI: 10.3969/j.issn.1006-6233.2020.01.031 Objective: To investigate the clinical effect of butylphthalide capsule in the treatment of post-stroke vascular dementia. Methods: 70 patients with vascular dementia hospitalized in our hospital from July 2015 to December 2017 were prospectively selected. They were divided into two groups according to simple random number table, 35 cases in each group. The control group received nimodipine orally for routine treatment.The clinical efficacy and adverse reactions during treatment were observed and compared between the two groups, cognitive function was evaluated by simple mental state scale (MMSE), (CDR) was used to evaluate the curative effect of dementia severity, and activity of daily living scale (ADL) was used to evaluate daily students before and after treatment. The changes of serum MMSE, CDR, ADL level before and after treatment were compared. Results: Compared with the clinical efficacy of the two groups, the total effective rate of the observation group was significantly higher than that of the control group (P< 0.05). The analysis of variance of repeated measurements showed that the interaction of time factor and time factor of MMSE, CDR, ADL score and grouping had statistical significance (P< 0.05). It showed that the differences of MMSE, CDR and ADL score before and after treatment in the observation group was different from those before and after the control group. The difference of MMSE, CDR and ADL score in the observation group was significantly greater than that in the control group before and after the treatment. The analysis of variance of repeated measurements showed that the interaction of time factors and time factors of SOD, MDA and BDNF levels and groups had statistical significance (P< 0.05). It showed that the differences of SOD, MDA and BDNF levels before and after treatment in the observation group were different from those before and after treatment in the control group. The differences of SOD, MDA and BDNF levels in the observation group before and after treatment were significantly greater than those in the control group. There were no serious adverse reactions related to this study. Conclusion: Butylphthalide capsule is effective in the treatment of post-stroke vascular dementia. It can significantly improve the degree of dementia, cognitive function and ability of daily living. No adverse reactions occurred during the treatment. It is safe and effective in clinical treatment, and is worthy of clinical application and promotion. 2020 Vol. 26 (1): 130-133 [Abstract] ( 116 ) HTML (1 KB)  PDF (1192 KB)  ( 369 ) 134 Analysis on the Incidence Rate and Influencing Factors of Postoperative Complications in Children with Hypospadias HU Wen DOI: 10.3969/j.issn.1006-6233.2020.01.032 Objective: To observe the incidence rate of postoperative complications in children with hypospadias, and analyze the influence factors. Methods: 300 children with hypospadias in the hospital were selected. All of them were treated with one-stage urethroplasty. The occurrence of postoperative complications was observed. The risk factors were analyzed by Logistic regression analysis. Results: Of the 300 children, there were 48 cases with urinary fistula, 5 cases with infection, 4 cases with flap necrosis and 5 cases with urethral stricture. The total incidence rate of complications was 20.67% (62/300). The incidence of postoperative complications in children with age not less than 2 years old, type III and type IV hypospadias, preoperative penile curvature, Koyanagi operation and Snodgrass operation was significantly higher than that with age less than 2 years, type I and type II, no preoperative penile curvature, Magpi operation, Onlay operation, Mathieu operation and Duckett operation (P<0.05). Logistic regression analysis showed that age not less than 2 years old, type III and type IV hypospadias, preoperative penile curvature, Koyanagi operation and Snodgrass operation were independent risk factors for postoperative complications in hypospadias children (P<0.05). Conclusion: The incidence of postoperative complications in hypospadias children is relatively higher, mainly on urinary fistula. Age, hypospadias type, preoperative penile curvature and surgical methods are all influencing factors of postoperative complications. Clinically, corresponding preventive measures can be taken against these factors. 2020 Vol. 26 (1): 134-137 [Abstract] ( 136 ) HTML (1 KB)  PDF (1180 KB)  ( 290 ) 138 Study on the Mechanism of Iucain Negulating TRAIL Gene to Inhibit the Proliferation and Induce Apoptosis of Cervical Cancer Cells JIN Cuihong, et al DOI: 10.3969/j.issn.1006-6233.2020.01.033 Objective: To investigate the effect of procaine on proliferation and apoptosis of cervical cancer cells and its mechanism. Methods: SiHa cells were treated with procaine with final concentrations of 0.0 mm / L, 0.5 mm / L, 1 mm / L, and 2 mm / L. pcDNA and pcDNA trail were transfected into untreated SiHa cells respectively, which were recorded as pcDNA group and pcDNA trail group. After Si NC and Si trail were transfected into SiHa cells, they were treated with procaine with 2 mm / L, which were recorded as procaine 2 mm / L + Si NC group and procaine 2 mm / L + Si trail group, respectively The liposome method was used. MTT method was used to detect cell proliferation; flow cytometry was used to detect apoptosis; QRT PCR was used to detect trail mRNA expression; Western blot was used to detect protein expression. Results: Procaine can inhibit the expression of CyclinD1, Bcl-2, p21, Caspase-8 and Bax, inhibit the proliferation of SiHa, promote apoptosis and promote the expression of trail. Overexpression of trail inhibited the proliferation of SiHa and promoted apoptosis. Inhibition of TRAIL expression reversed the inhibition of proliferation and apoptosis of cervical cancer cell line SiHa by procaine. Conclusion: Procaine can inhibit the proliferation of SiHa and promote the apoptosis of cervical cancer cells, which may be related to the regulation of trail. It will provide new ideas and targets for the treatment of cervical cancer. 2020 Vol. 26 (1): 138-142 [Abstract] ( 131 ) HTML (1 KB)  PDF (1944 KB)  ( 310 ) 143 Relationship between the Position of the Tip of the Central Venous Catheter Positioned by Ultrasound and Flow Rate DU Yue, LIU Huiling, DING Huajie, et al DOI: 10.3969/j.issn.1006-6233.2020.01.034 Objective: To analyze the relationship between the position of catheter tip and flow rate in patients undergoing transjugular central venous catheter placement. Methods: 225 patients with maintenance hemodialysis were treated with transjugular central venous catheterization. According to the position of the tip of the catheter, the Group 1 is located in the superior vena cava, the Group 2 is located in the upper middle level of the right atrium (including the upper vena cava into the right atrium level), and the Group 3 is in the other locations within the heart chamber. The actual pump flow (ml/min) at the arteriovenous end of the catheter within 1 hour after the first use of CVC for dialysis within 24 hours after surgery was recorded, and the flow between the groups was compared. Results: The median blood flow of the Group 1 was 240 ml/min. The median blood flow of the Group 2 was 250 ml/min, and the median blood flow of the Group 3 was 230 ml/min, there was a statistically significant difference (P<0.05) in catheter flow between the three groups with different catheter tip positions. Further comparison between the groups was made, p (Group 1/Group 2)=0.001、p (Group 2/Group 3) = 0.000, difference was statistically significant. Conclusion: The dialysate flow of the central venous catheter through the internal jugular vein is related to the tip position of the catheter. The best level of blood flow is in the upper middle part of the right atrium. Ultrasound monitoring the tip position of the catheter has a certain value. 2020 Vol. 26 (1): 143-145 [Abstract] ( 175 ) HTML (1 KB)  PDF (1196 KB)  ( 459 ) 150 Effects Analysis of Clinical Curative Effect and Immune Function on Inflammatory on Infliximab Combined with Traditional Chinese Medicine Bowel Disease Treatment during Active Stage LIU Wei DOI: 10.3969/j.issn.1006-6233.2020.01.036 Objective: To study effects of infliximab (IFX) combined with traditional Chinese medicine (TCM) on clinical curative effect and immune function in inflammatory bowel disease (IBD) treatment during active stage. Methods: 94 IBD patients during active stage were enrolled. According to simple random and voluntary principle, they were divided into control group (8 cases) and study group (56 cases). The control group was treated with IFX, while study group was treated with TCM on basis of control group. The inflammatory cytokines, lymphocyte subsets, scores of TCM syndromes and clinical curative effect before and after treatment was compared between the two groups. Results: The total response rate of treatment in study group was significantly higher than that in control group (χ2=4.351, P<0.05). Before treatment, there were no significant differences in levels of inflammatory factors (CRP, IL-6, TNF-α) between the two groups (P>0.05). After treatment, decrease amplitude of the above indexes in study group was significantly greater than that in control group (P<0.05). Before treatment, there were no significant differences in levels of lymphocyte subsets (NK cells, CD3+, CD4+, CD4+/CD8+) between the two groups (P>0.05). After treatment, increase amplitude of the above indexes in study group was significantly greater than that in control group (P<0.05). Before treatment, scores of TCM syndromes in control group and study group were (14.96±4.50) points and (15.24±4.16) points, respectively (P>0.05). After treatment, scores of TCM syndromes in control group and study group were (7.34±0.68) points and (5.65±0.49) points. Compared with that before treatment, score decrease amplitude in study group was significantly greater than that in the control group (P<0.05). Conclusion: The curative effect of IFX combined with Anchang Yuyang decoction is significant, which can reduce levels of inflammatory factors, enhance levels of lymphocyte subsets, improve total response rate of treatment, improve immune function, reduce scores of TCM syndromes and effectively treat IBD patients during active stage. 2020 Vol. 26 (1): 150-153 [Abstract] ( 139 ) HTML (1 KB)  PDF (1205 KB)  ( 296 ) 154 Effect of Astragalus Membranaceus on Cytokines and HSP70 in Children with Viral Myocarditis NIU Ling, AN Xinjiang, XU Hui, et al DOI: 10.3969/j.issn.1006-6233.2020.01.037 Objective: To observe the effect of Astragalus membranaceus on cytokines and HSP70 in children with viral myocarditis (VMC). Methods: 70 children with viral myocarditis clinically confirmed in our hospital from April 2016 to March 2018 were selected as research objects, randomly divided into 38 cases in the research group and 32 cases in the control group. The control group used conventional treatment, observation group in the conventional treatment on the basis of oral huangqi granules. Venous blood of the children was extracted one week later, and the following indicators, il-1, il-6, ifn-gamma, tnf-alpha and HSP70, were detected respectively. Results: 1. Il-1, il-6, ifn-gamma and tnf-alpha of the children in the study group and the control group were significantly decreased after treatment compared with those before treatment (P<0.05), with statistically significant differences. Il-1, il-6, ifn-gamma and tnf-alpha of the children in the study group decreased significantly after treatment compared with that of the control group (P<0.05), with statistically significant differences. 2. HSP70 level of children in the study group and control group increased significantly after treatment compared with that before treatment; The level of HSP70 in the study group was significantly different from that in the control group (P<0.05). Conclusion: Astragalus can reduce the concentration of il-1, il-6, ifn-gamma and tnf-alpha in children with viral myocarditis, and up-regulate the level of HSP70. 2020 Vol. 26 (1): 154-157 [Abstract] ( 143 ) HTML (1 KB)  PDF (1214 KB)  ( 300 ) 158 Comparison on Efficacy of Docetaxel, Irinotidine and Tiggio in Patients with Advanced Gastric Cancer WANG Wenming, DUAN Aixiong, JIANG Qi'an, et al DOI: 10.3969/j.issn.1006-6233.2020.01.038 Objective: To investigate the clinical efficacy and safety of docetaxel combined with irinotecan and irinotecan combined with teggio in patients with advanced gastric cancer. Methods: 35 patients with advanced gastric cancer receiving chemotherapy with docetaxel combined with tiggio in the department of oncology of our hospital between September 2013 and April 2018 were selected as the experimental group, and 25 patients receiving irinotecan combined with tiggio as the control group. The disease control rate and toxicity reaction after chemotherapy were compared between the two groups. The clinical value of docetaxel and irinotidine combined with teggio in patients with advanced gastric cancer were evaluated. Results: The tumor remission rate and control rate of the experimental group were 34.29% and 71.43%, respectively, while those of the control group were 36% and 72%. The difference between the two groups was not statistically significant (χ2=0.019, 0.002, P>0.05). After chemotherapy toxicity of two groups of reactions including leukopenia, thrombocytopenia, nausea and vomiting, hair loss, neurotoxicity, and extremities syndrome, etc., and all kinds of reaction is given priority to with 0 ~ Ⅱ degrees, differences were not statistically significant (P>0.05). Conclusion: Docetaxel combined with tegeor and irinotecan combined with tegeor can effectively control the development speed of cancer patients, reduce the occurrence of toxic reactions, and both of them have the same effect, and the patients with toxic reactions can tolerate, which is worthy of clinical application. 2020 Vol. 26 (1): 158-161 [Abstract] ( 316 ) HTML (1 KB)  PDF (1195 KB)  ( 387 ) 162 Effect of Wuling Capsule on Clinical Efficacy and Negative Emotion in Patients with Post-stroke Depression WANG Shijun, LUAN Shuxin, WAN Hongquan, et al DOI: 10.3969/j.issn.1006-6233.2020.01.039 Objective: To investigate the effect of Wuling Capsule on the efficacy and safety of post-stroke depression patients. Methods: The subjects were 90 patients with post-stroke depression who were treated in our hospital from June 2017 to July 2018. They were simply randomly divided into treatment group (45 cases) and control group (45 cases). In the control group, patients with post-stroke depression were treated with western medicine fluoxetine, while in the treatment group, patients were treated with traditional Chinese medicine Wuling capsule. Through the treatment of two different schemes, the treatment effects of the control group and the treatment group were compared and analyzed. It mainly includes the improvement of clinical indicators and clinical treatment effect, the change of cytokines in patients after treatment and the adverse reactions of patients after treatment. Results: The HAMD score and Bi score of the treatment group were significantly higher than those of the control group (P < 0.05); the total effective rate of the treatment group was 91.67%, significantly higher than 70.00% of the control group (P < 0.05); the cytokine IL-6 and TNF - α of the treatment group were significantly lower than those of the control group (P < 0.05); compared with the control group, The rate of adverse reactions in the treatment group was significantly lower than that in the control group (P < 0.05). Conclusion: Wuling Capsule improves the clinical efficacy of patients with post-stroke depression. In terms of adverse reactions, Wuling Capsule also has a certain improvement effect. 2020 Vol. 26 (1): 162-165 [Abstract] ( 151 ) HTML (1 KB)  PDF (1189 KB)  ( 313 ) 166 Clinical Observation of Three Different Drugs for Treatment of Lower Ureteral Calculi CAO Xue, LI Honglu, TONG Xiaoshu DOI: 10.3969/j.issn.1006-6233.2020.01.040 Objective: To observe the clinical efficacy of three different drugs (tamsulosin, nifedipine and racemic anisodamine) in the treatment of lower ureteral calculi. Methods: 90 patients with lower ureteral calculi in our hospital from January 2016 to December 2018 were randomly divided into tamsulosin group, nifedipine group, racemic anisodamine group and control group, with 22 cases, 23 cases, 22 cases and 23 cases respectively. All four groups needed a large amount of water to ensure the daily total urine volume > 2000 ml, of which tamsulosin group. Group B, nifedipine group and racemic anisodamine group were given 0.4 mg po qd, 10 mg potid and 10 mg po tid, while blank control group was not given drug intervention for 2 weeks. To observe and compare the clinical efficacy, the size of stone removal, the time of stone removal, the size of residual stones and adverse reactions of each group. Results: The total effective rate was tamsulosin group > racemic anisodamine group > nifedipine group > control group (P<0.05); lithotripsy size was tamsulosin group > racemic anisodamine group > nifedipine group > control group (P<0.05); lithotripsy time was tamsulosin group < nifedipine group < racemic anisodamine group < control group (P<0.05). There was no significant difference between nifedipine group and racemic anisodamine group (P>0.05); residual stone size of tamsulosin group < nifedipine group < racemic anisodamine group < control group (P<0.05); no significant adverse reactions occurred in the four groups during the experiment. Conclusion: Tamsulosin has better clinical efficacy than nifedipine and racemic anisodamine in the treatment of lower ureteral calculi. Nifedipine and racemic anisodamine have similar therapeutic effects, and the three groups have better safety. 2020 Vol. 26 (1): 166-168 [Abstract] ( 149 ) HTML (1 KB)  PDF (1193 KB)  ( 292 ) 169 Study on Drug Resistance Distribution and Genotyping of Streptococcus Pneumoniae in Children with Influenza A in A Tertiary Hospital ZHU Jun, KONG Yan, SHI Xiaodi, et al DOI: 10.3969/j.issn.1006-6233.2020.01.041 Objective: To investigate the distribution and genotyping of Streptococcus pneumoniae (SPN) resistance in children with influenza A in a class a hospital of grade III. Methods: 476 children with influenza A treated in Gaochun People's Hospital of Nanjing City were selected from January 2016 to January 2019, samples of pharyngeal swab or nasal swab were collected, after identification of Streptococcus pneumoniae by OPTOXIN test and automatic microbiological identification instrument, the agar dilution method was used to confirm the susceptibility of bacteria by automatic bacterial identification/susceptibility system, and polymerase chain reaction (PCR) was used to detect the drug resistance genotypes of Streptococcus pneumoniae. Results: 36 strains of Streptococcus pneumoniae (7.56%) were isolated from throat swabs or nasal swabs of 476 children with influenza A. Among them, 35 strains (97.22%) were resistant to erythromycin, 31 strains (86.11%) were resistant to clindamycin, 36 strains (100.00%) were resistant to penicillin, 3 strains (8.33%) were resistant to vancomycin, 30 strains (83.33%) were resistant to azithromycin, 32 strains (88.11%) were resistant to ceftazidime. 89%, 1 strain (2.78%) was resistant to meropenem, 36 strains (100.00%) were resistant to tetracycline, 11 strains (30.56%) were resistant to linezolid and 36 strains (47.22%) were resistant to moxifloxacin. 36 strains of Streptococcus pneumoniae contained 32 strains of pbp2B resistance gene (88.89%), 17 strains of ermA resistance gene (47.22%), 21 strains of mefB resistance gene (58.33%) and 30 strains of tetM resistance gene (83.33%). The mutations of pbp2B, ermA, mefB and tetM genes were highly consistent with their drug resistance phenotypes, the detection rates of pbp2B gene in Streptococcus pneumoniae resistant to penicillin and ceftazidime were 88.89% and 100.00% respectively, the detection rates of either or both of ermA and mefB genes exist simultaneously in erythromycin-resistant and azithromycin-resistant Streptococcus pneumoniae were 97.14% and 93.33%, respectively, and the detection rate of tetM gene in tetracycline-resistant Streptococcus pneumoniae was 83.33%. Conclusion: Streptococcus pneumoniae is highly resistant to penicillin, erythromycin, tetracycline and other antibiotics. Its resistance is related to the expression of related resistance genes (pbp2B, ermA, mefB, tetM). Etiological detection should be strengthened to facilitate rational selection of antibiotics. 2020 Vol. 26 (1): 169-172 [Abstract] ( 95 ) HTML (1 KB)  PDF (1215 KB)  ( 367 ) 173 Effects of Warfarin on Coagulation Platelet Parameters and Hemorheology in Patients with Atrial Fibrillation BAI Chongyang, ZHANG Huizhong DOI: 10.3969/j.issn.1006-6233.2020.01.042 Objective: To investigate the coagulation function, platelet parameters and hemorheology of warfarin in patients with atrial fibrillation. Methods: 94 patients with atrial fibrillation were enrolled from our hospital from October 2016 to October 2018. The patients were simple random divided into two groups, 47 in each group. One group was the control group and treated with aspirin. The other group was the observation group, which was treated with warfarin. The changes of coagulation function, platelet parameters and blood rheology related indexes before and after treatment were compared between the two groups. Results: After treatment, the coagulation function related indicators, partial activated thrombin time (APTT), thrombin time (TT) and prothrombin time (PT) were significantly higher in the observation group than in the control group (P<0.05). Platelet-related indicators in the observation group after treatment, platelet surface membrane glycoprotein IIb/IIIa (GPIIb/IIIa), platelet alpha granule membrane glycoprotein (CD62p), platelet membrane glycoprotein fibrinogen receptor (PAC-1) were significantly lower In the control group (P<0.05). After treatment, the fibrinogen (FIB), low blood viscosity and high blood viscosity of the observation group were significantly lower than those of the control group (P<0.05). The difference between the two groups was statistically significant. Conclusion: Warfarin in patients with atrial fibrillation can effectively improve blood coagulation, platelet and blood rheology, has clinical application value. 2020 Vol. 26 (1): 173-176 [Abstract] ( 152 ) HTML (1 KB)  PDF (1184 KB)  ( 294 )

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