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2024 Vol. 30, No. 8
Published: 2024-08-31
1233
Role of the NKILA-miR-889-3p Axis in Regulating Proliferation Migration and Invasion of Oral Squamous Cell Carcinoma Cells
YANG Shuo, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.01
Objective:
To analyze the expression and clinical significance of the long non-coding RNA NKILA in oral squamous cell carcinoma (OSCC), investigate the regulatory mechanism between NKILA and its potential target miRNA, and elucidate its role in OSCC development.
Methods:
Eighty OSCC patients confirmed by pathology and corresponding adjacent normal oral mucosa tissue samples were collected. The expression level of NKILA and its correlation with prognosis were detected using qRT-PCR. The effects of NKILA on OSCC cell proliferation, migration, and invasion were evaluated through colony formation assays and Transwell assays. Subcellular fractionation experiments were conducted to determine the subcellular localization of NKILA. LncBase was used to predict potential target miRNAs of NKILA. AGO
2
immunoprecipitation experiments were performed to validate the interaction between miR-889-3p and NKILA.
Results:
NKILA expression was significantly downregulated in OSCC tissues compared to adjacent normal tissues (P<0.0001). Low expression of NKILA was significantly associated with advanced TNM stage, lymph node metastasis, and distant metastasis in OSCC patients (P=0.001, P=0.046, P=0.011). Cell experiments demonstrated that knockdown of NKILA significantly promoted OSCC cell migration and invasion (P<0.05), while overexpression of NKILA significantly inhibited these cellular functions (P<0.05). Subcellular localization experiments showed that NKILA primarily localized in the cytoplasm. miR-889-3p was identified as a potential target of NKILA, and overexpression of NKILA significantly upregulated miR-889-3p expression (P<0.05), and vice versa. AGO2 immunoprecipitation experiments further confirmed that miR-889-3p specifically binds to and regulates NKILA (P<0.05).
Conclusion:
This study elucidates the downregulation of NKILA in OSCC development and its potential functional mechanisms. It reveals an important regulatory interaction between NKILA and miR-889-3p, providing new insights for the study and treatment of OSCC.
2024 Vol. 30 (8): 1233-1238 [
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1238
Effects of LncRNA SNHG16 on the Proliferation Migration and Invasion of Esophageal Cancer Cells via the miR-212-3p/FAM3C Axis
HOU Yingli, FENG Xiaona, LI Chunhui, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.02
Objective:
To investigate the effect of long non-coding small nucleolar RNA host gene 16 (LncRNA SNHG16) targeting microRNA-212-3p (miR-212-3p)/Family with sequence similarity 3 member C (FAM3C) axis on the proliferation, migration, and invasion of esophageal cancer cells.
Methods:
Human esophageal cancer cells KYSE-510 were cultured in vitro and divided into five groups: control group, si-NC group, si-SNHG16 group, si-SNHG16+inhibitor NC group, and si-SNHG16+miR-212-3p inhibitor group. The expression levels of LncRNA SNHG16, miR-212-3p, and FAM3C mRNA were detected using RT-qPCR. The proliferation of KYSE-510 cells was assessed using the CCK-8 assay, apoptosis was analyzed by flow cytometry, migration was evaluated by wound healing assay, and invasion was measured by Transwell assay. The dual-luciferase reporter assay was used to verify the targeting relationship between LncRNA SNHG16 and miR-212-3p, as well as between miR-212-3p and FAM3C.
Results:
Compared with the control and si-NC groups, the si-SNHG16 group showed decreased levels of LncRNA SNHG16 and FAM3C mRNA, reduced OD values, lower wound healing rates, decreased invasion numbers, and increased miR-212-3p levels and apoptosis rates (P<0.05). Compared with the si-SNHG16+inhibitor NC group, the si-SNHG16+miR-212-3p inhibitor group showed decreased miR-212-3p levels and apoptosis rates, and increased FAM3C mRNA levels, OD values, wound healing rates, and invasion numbers (P<0.05), with no difference in LncRNA SNHG16 levels (P>0.05). Bioinformatics prediction revealed the presence of target binding sites between miR-212-3p and both LncRNA SNHG16 and FAM3C, which was further validated by the dual-luciferase reporter assay (P<0.05).
Conclusion:
LncRNA SNHG16 is upregulated in esophageal cancer. Knockdown of LncRNA SNHG16 can target and upregulate miR-212-3p, inhibiting the expression of FAM3C, thereby suppressing the proliferation, migration, and invasion of esophageal cancer cells and promoting apoptosis.
2024 Vol. 30 (8): 1238-1244 [
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1244
Effects of GLA on IL-1β-Induced Autophagy and Apoptosis in Articular Chondrocytes via PINK1/Parkin Pathway
GAO Xiang, LI Wei, CHEN He
DOI: 10.3969/j.issn.1006-6233.2024.08.03
Objective:
To investigate the effect of geniposide (GLA) on PTEN-induced kinase 1 (PINK1)/E3 ubiquitin ligase (Parkin) signaling pathway in regulating autophagy and apoptosis of articular chondrocytes induced by interleukin-1β (IL-1β).
Methods:
Articular chondrocytes were divided into Control, IL-1β, Low-GLA, Medium-GLA, High-GLA, and Suramin groups. Cell proliferation was assessed using the Cell Counting Kit-8 (CCK8) assay. Autophagy was detected by transmission electron microscopy (TEM). Apoptosis was measured by flow cytometry. Levels of inflammatory factors (MMP3, TNF-α, MMP13) and reactive oxygen species (ROS) in chondrocytes were detected by ELISA. Protein expressions of cleaved caspase-3, microtubule-associated protein 1 light chain 3 (LC3) I, LC3II, P62, PINK1, and Parkin were analyzed by Western blot.
Results:
Compared with the Control group, the IL-1β group showed decreased cell viability, LC3II/I ratio, PINK1, and Parkin, while autophagic vacuole number, apoptosis rate, MMP3, TNF-α, MMP13, ROS, cleaved caspase-3, and P62 were increased (P<0.05). The Low-GLA, Medium-GLA, and High-GLA groups exhibited higher cell viability, autophagic vacuole number, LC3II/I ratio, PINK1, and Parkin than the IL-1β group, with lower apoptosis rate, MMP3, TNF-α, MMP13, ROS, cleaved caspase-3, and P62 (P<0.05). The Suramin group showed lower cell viability, autophagic vacuole number, LC3II/I ratio, PINK1, and Parkin than the High-GLA group, with higher apoptosis rate, MMP3, TNF-α, MMP13, ROS, cleaved caspase-3, and P62 (P<0.05).
Conclusion:
GLA inhibits the progression of osteoarthritis (OA) by activating autophagy and inhibiting apoptosis in IL-1β-induced articular chondrocytes, possibly through the activation of the PINK1/Parkin signaling pathway.
2024 Vol. 30 (8): 1244-1249 [
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12
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1250
Hypoxia Promotes the Molecular Mechanism of High-Altitude Polycythemia by Mediating the Demethylation of Hepcidin mRNA through HIF-1α
BAI Jie, HUANG He, ZHANG Lian, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.04
Objective:
To investigate the roles of HIF-1α and Hepcidin in the pathogenesis of high-altitude polycythemia (HAP).
Methods:
Peripheral blood hemoglobin concentration [Hb], hemoglobin mass (Hbmass), blood volume, and peripheral blood O
2
saturation (SpO
2
) were measured in subjects with high-altitude polycythemia (HAE group), healthy individuals residing at the same high altitude (HH group), and healthy individuals born at high altitude but residing at low altitude for over 2 years (LH group). ELISA was used to determine the levels of HIF-1α and Hepcidin in peripheral blood serum. HepG2 cells were cultured under hypoxia in vitro, with experimental groups including Control and Hypoxia groups. The expression levels of HIF-1α and Hepcidin mRNA and protein were measured. m6A content analysis, RNA immunoprecipitation (RIP), dual-luciferase reporter assay, and mRNA stability analysis were used to explore the molecular mechanisms by which HIF-1α mediates m6A demethylation of Hepcidin mRNA.
Results:
Compared with the LH group, the HH group had increased levels of [Hb], Hbmass, and blood volume, and decreased SpO
2
; compared with the HH group, the HAE group had increased levels of [Hb], Hbmass, and blood volume, and decreased SpO
2
(P<0.05). Compared with the Control group, the Hypoxia group showed increased relative expression levels of HIF-1α mRNA and protein, and decreased relative expression levels of Hepcidin mRNA and protein in HepG2 cells (P<0.05). The m6A (%) level in the Hypoxia group was lower than in the Control group (P<0.05). mRNA stability analysis showed that the relative expression level of Hepcidin mRNA in the Hypoxia group was lower than in the Control group (P<0.05). Dual-luciferase reporter assay showed that relative luciferase activity was higher in HepG2 cells co-transfected with Hepcidin 3'-UTR and HIF-1α shRNA compared to cells co-transfected with Hepcidin 3'-UTR and shRNA NT (P<0.05). RIP results showed that the enrichment level of Hepcidin 3'-UTR was higher in the FTO Ab group compared to the IgG group (P<0.05).
Conclusion:
Hypoxia-induced upregulation of HIF-1α mediates the demethylation of Hepcidin mRNA, contributing to the progression of high-altitude polycythemia.
2024 Vol. 30 (8): 1250-1255 [
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11
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1255
Stasis-Removing Method on Glucose and Lipid Metabolism and the IRS-1/PI3K/Akt2 Signaling Pathway in an Obese Type 2 Diabetes Mellitus Model
DUN Zhihua, QIU Yaru, WEI Xiufeng, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.05
Objective:
To explore the effects and mechanisms of the spleen-fortifying, phlegm-resolving, and stasis-removing method on glucose and lipid metabolism in rats with type 2 diabetes mellitus (T2DM).
Methods:
T2DM was induced in rats using a high-fat diet combined with a low dose of streptozotocin. The rats were divided into control, model, and three treatment groups with low, medium, and high doses of the spleen-fortifying, phlegm-resolving, and stasis-removing formula. Serum inflammatory cytokines, biochemical indicators, and key enzyme activities related to glucose metabolism in the liver were measured using ELISA kits. mRNA levels of key insulin signaling pathway targets were detected by qPCR. Western blotting was used to assess the expression of phosphatidylinositol 3-kinase (PI3K), phosphorylated PI3K (p-PI3K), protein kinase B (Akt), phosphorylated Akt (p-Akt), and glucose transporter 2 (Glut2) in the liver.
Results:
Compared with the control group, the model group had significantly elevated serum levels of fasting blood glucose (FBG), fasting insulin (FINS), total cholesterol (TC), triglycerides (TG), free fatty acids (FFA), and low-density lipoprotein cholesterol (LDL-C), and significantly reduced high-density lipoprotein cholesterol (HDL-C). The treatment with the spleen-fortifying, phlegm-resolving, and stasis-removing formula significantly reduced FINS, TG, TC, LDL-C, and FFA levels, while increasing HDL-C levels compared with the model group. Additionally, serum levels of CRP, IFN-γ, TNF-α, IL-6, IL-1β, and NO were significantly higher in the model group compared to the control group, but were significantly reduced after treatment. The activities of PEPCK, FBPase, G6Pase, and GP enzymes were elevated in the model group but decreased significantly following treatment. Moreover, the expression of IRS-1, p-PI3K, and p-Akt2 was increased in the model group but significantly reduced with the treatment.
Conclusion:
The spleen-fortifying, phlegm-resolving, and stasis-removing method may improve glucose and lipid metabolism in T2DM rats by activating the IRS-1/PI3K/Akt2 signaling pathway.
2024 Vol. 30 (8): 1255-1261 [
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1261
Effect of Hyperoside on Neuroinflammation in Bacterial Meningitis Rats by Regulating the TXNIP/NLRP3 Signaling Pathway
CHEN Jie, JIANG Huilin, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.06
Objective:
To analyze the effects of Hypericin (Hyp) on neuroinflammation in bacterial meningitis (BM) rats by regulating the thioredoxin-interacting protein (TXNIP)/nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) signaling pathway.
Methods:
Fifteen healthy rats were randomly selected as the control group (tail vein injection of saline). BM model rats were randomly divided into BM group (tail vein injection of saline), L-Hyp group (tail vein injection of 10mg/kg Hyp), H-Hyp group (tail vein injection of 50mg/kg Hyp), TXNIP-AAV group (tail vein injection of TXNIP-AAV), AAV group (tail vein injection of AAV-NC), and Resveratrol (Res) group (tail vein injection of 30mg/kg Res). Neurological function after treatment was evaluated using the Loeffler score; white blood cell (WBC) count was determined using a blood cell analyzer; reactive oxygen species (ROS), interleukin (IL)-1β, IL-6, and tumor necrosis factor-alpha (TNF-α) levels in cerebrospinal fluid (CSF) were analyzed by ELISA; brain tissue water content was measured; pathological changes in brain tissue were observed using HE staining; cell apoptosis in brain tissue was observed using TUNEL staining; and protein expressions of TXNIP, NLRP3, caspase-1, apoptosis-associated speck-like protein (ASC), ionized calcium-binding adaptor molecule 1 (Iba1), and IL-1β in brain tissue were analyzed using Western blotting.
Results:
Compared with the control group, the BM group showed increased levels of ROS, WBC, IL-1β, IL-6, and TNF-α in CSF, increased brain tissue water content and cell apoptosis rate, and decreased Loeffler scores (P<0.05). Compared with the BM group, the L-Hyp and H-Hyp groups showed increased Loeffler scores, and decreased levels of ROS, WBC, IL-1β, IL-6, and TNF-α in CSF, brain tissue water content, and cell apoptosis rate (P<0.05). Compared with the H-Hyp group, the TXNIP-AAV and AAV groups showed increased levels of ROS, WBC, IL-1β, IL-6, and TNF-α in CSF, brain tissue water content, and cell apoptosis rate, and decreased Loeffler scores (P<0.05), whereas the Res group showed increased Loeffler scores and decreased levels of ROS, WBC, IL-1β, IL-6, and TNF-α in CSF, brain tissue water content, and cell apoptosis rate (P<0.05). The brain tissues of rats in the control group showed normal morphology; in the BM, TXNIP-AAV, and AAV groups, brain tissues showed morphological changes, disordered cell arrangement, and nuclear shrinkage; in the L-Hyp, H-Hyp, and Res groups, brain tissues showed improved morphology, with reduced cell necrosis and shrinkage. Compared with the control group, the BM group showed increased expressions of TXNIP, NLRP3, caspase-1, ASC, Iba1, and IL-1β in brain tissues (P<0.05). Compared with the BM group, the L-Hyp and H-Hyp groups showed decreased expressions of TXNIP, NLRP3, caspase-1, ASC, Iba1, and IL-1β in brain tissues (P<0.05). Compared with the H-Hyp group, the TXNIP-AAV and AAV groups showed increased expressions of TXNIP, NLRP3, caspase-1, ASC, Iba1, and IL-1β in brain tissues, while the Res group showed decreased expressions of these proteins (P<0.05).
Conclusion:
Hypericin may inhibit neuroinflammation in BM rats, possibly through inhibition of the TXNIP/NLRP3 pathway.
2024 Vol. 30 (8): 1261-1267 [
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1267
Regulation of Th17/Treg Balance by BAFF in a Mouse Model of Immune Thrombocytopenia
LI Wei, MA Xihu, LIU Xiao, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.07
Objective:
To explore the regulatory role of B-cell activating factor (BAFF) on the balance of helper T cell 17 (Th17) and regulatory T cell (Treg) in vivo in a mouse model of immune thrombocytopenia (ITP).
Methods:
Guinea pig anti-mouse platelet serum (GP-APS) was prepared, and 150 adult male BALB/c mice (7-8 weeks old) were randomly divided into 5 groups (n=30 per group): control (blank control), ITP (induced by GP-APS), ITP+rhBAFF (ITP mice treated with intravenous injection of recombinant human BAFF protein at 50μg/kg/50μL), ITP+rhBAFF+DAPT (combined with Notch1 inhibitor DAPT), and ITP+rhBAFF+PGD (combined with PI3K/Akt inhibitor Polygalacin D). DAPT was injected at 100μg/kg, and PGD at 25 μg/kg, both with a total volume of 50μL and administered once daily. Peripheral blood (1mL) was collected from mice after 1 week for serum and mononuclear cell isolation. Immunofluorescence staining was used to detect BAFF and Notch1 localization in mononuclear cells. Platelet counts in peripheral blood were determined. Enzyme-linked immunosorbent assay (ELISA) was performed to measure BAFF levels in mouse serum. Western blotting was conducted to assess protein expression of PI3K, AKT, Notch1, p-Akt(Thr308), and p-Akt(Ser473) in mononuclear cells. Flow cytometry was used to analyze changes in Th17/Treg ratio in mononuclear cells.
Results:
BAFF and Notch1 were co-localized on the cell membrane of mononuclear cells in peripheral blood of ITP mice. Compared with the control group, ITP group showed increased expression of BAFF, Notch1, p-Akt(Thr308), p-Akt(Ser473), increased Th17 proportion, decreased platelet count, and decreased Treg proportion (P<0.05). Compared with the ITP group, ITP+rhBAFF group exhibited increased BAFF, Notch1, p-Akt(Thr308), p-Akt(Ser473) expression, increased Th17 proportion, decreased platelet count, and decreased Treg proportion (P<0.05). Compared with the ITP+rhBAFF group, both ITP+rhBAFF+DAPT and ITP+rhBAFF+PGD groups showed decreased BAFF, Notch1, p-Akt(Thr308), p-Akt(Ser473) expression, increased platelet count, increased Treg proportion, and decreased Th17 proportion (P<0.05).
Conclusion:
BAFF promotes an increase in Th17 proportion and decrease in Treg proportion in a mouse model of immune thrombocytopenia by activating the Notch1/PI3K/Akt signaling pathway.
2024 Vol. 30 (8): 1267-1272 [
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1272
Effects of Leonurine on Aortic Endothelial Cell Injury in Rats with Coronary Heart Disease by Regulating the RhoA/ROCK Signaling Pathway
GUO Yanhong, XIE Yuemin, FENG Qiang, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.08
Objective:
To investigate the effect and mechanism of leonurine (Leo) on aortic endothelial cell injury in rats with coronary heart disease (CHD) by regulating the Ras homolog gene family member A (RhoA)/Rho-associated coiled-coil containing protein kinase (ROCK) signaling pathway.
Methods:
Seventy-five successfully modeled CHD rats were randomly divided into five groups: model group (CHD group), low, medium, and high dose Leo groups (Leo-L, Leo-M, Leo-H groups), and Leo-H dose group combined with RhoA activator LPA group (Leo-H+LPA group), with 15 rats in each group. The Leo-L, Leo-M, and Leo-H groups were gavaged with 15, 30, and 60mg·kg
-1
·d
-1
of Leo, respectively. The Leo-H+LPA group was gavaged with 60mg·kg
-1
·d
-1
of Leo and intraperitoneally injected with 1mg·kg
-1
·d
-1
of LPA. The Control and CHD groups were administered equal volumes of normal saline in the same manner for four weeks. Cardiac function indicators were measured by echocardiography. Total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) levels were measured using an automatic biochemical analyzer. Enzyme-linked immunosorbent assay (ELISA) was used to detect levels of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), nitric oxide (NO), endothelin-1 (ET-1), endothelial cell-specific molecule-1 (EMS1), and vascular cell adhesion molecule-1 (VCAM-1). Hematoxylin and eosin (HE) staining was used to observe aortic endothelial damage. Flow cytometry was used to detect apoptosis of aortic endothelial cells. Western blot analysis was used to detect the expression of proteins related to the RhoA/ROCK signaling pathway.
Results:
Compared with the Control group, the CHD group showed significant aortic wall thickening, uneven staining, cell swelling, disordered arrangement, and rough endothelium; significant increases in left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD), TC, TG, LDL-C, IL-6, TNF-α, ET-1, VCAM-1, EMS1 levels, cell apoptosis rate, and RhoA, ROCK1, and ROCK2 protein expression (P<0.05); and significant decreases in left ventricular ejection fraction (LVEF), fractional shortening (FS), HDL-C, and NO levels (P<0.05). Compared with the CHD group, the Leo-L, Leo-M, and Leo-H groups showed uniform aortic staining, clear arterial wall structure, tightly arranged and morphologically normal cells, and smoother endothelium; significant decreases in LVEDD, LVESD, TC, TG, LDL-C, IL-6, TNF-α, ET-1, VCAM-1, EMS1 levels, cell apoptosis rate, and RhoA, ROCK1, and ROCK2 protein expression (P<0.05); and significant increases in LVEF, FS, HDL-C, and NO levels (P<0.05). The Leo-H+LPA group reversed the protective effects of Leo on CHD rats (P<0.05).
Conclusion:
Leonurine may improve cardiac function, reduce blood lipids, and inhibit inflammation in CHD rats by inhibiting the RhoA/ROCK signaling pathway, thereby alleviating aortic endothelial cell injury.
2024 Vol. 30 (8): 1272-1279 [
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1279
Impact of Tangeretin on TXNIP/NLRP3 Signaling Pathway Regulation and Cell Pyroptosis in Severe Pneumonia Rats
ZHOU Hong, ZHANG Peipei, LIU Huixue, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.09
Objective:
This study aimed to explore the influence of tangeretin on the TXNIP/NLRP3 signaling pathway in regulating cell pyroptosis in severe pneumonia rats.
Methods:
Sprague-Dawley rats were divided into control group, severe pneumonia group, low-dose tangeretin group, high-dose tangeretin group, high-dose tangeretin + pcDNA group, and high-dose tangeretin + pcDNA-TXNIP (TXNIP activator) group, with 12 rats in each group. Except for the control group, all other groups were induced to establish a severe pneumonia rat model by tracheal instillation of Klebsiella pneumoniae. Drug administration began immediately after successful modeling and continued for 4 weeks. Lung function parameters including peak expiratory flow (PEF) and forced vital capacity (FVC) were monitored using an animal lung function meter. Levels of interleukin (IL)-1β and IL-18 in bronchoalveolar lavage fluid were measured by ELISA. Changes in lung wet-to-dry weight ratio were evaluated. Lung histopathological changes were assessed by HE staining. Cell pyroptosis in lung tissues was detected using propidium iodide (PI) staining. Expression levels of TXNIP, NLRP3, cleaved caspase-1, and N-terminal fragment of gasdermin D (GSDMD-N) in lung tissues were analyzed by Western blot.
Results:
Compared with the control group, rats in the severe pneumonia group showed severe lung tissue damage, decreased PEF and FVC, increased levels of IL-1β and IL-18 in bronchoalveolar lavage fluid, increased lung wet-to-dry weight ratio, elevated cell pyroptosis rate in lung tissues, and increased expression of TXNIP, NLRP3, cleaved caspase-1, and GSDMD-N proteins (P<0.05). Compared with the severe pneumonia group, rats in the low-dose tangeretin and high-dose tangeretin groups exhibited improved lung tissue damage, increased PEF and FVC, decreased levels of IL-1β and IL-18 in bronchoalveolar lavage fluid, decreased lung wet-to-dry weight ratio, reduced cell pyroptosis rate in lung tissues, and decreased expression of TXNIP, NLRP3, cleaved caspase-1, and GSDMD-N proteins (P<0.05). The protective effect of high-dose tangeretin on severe pneumonia was reversed by pcDNA-TXNIP.
Conclusion:
Tangeretin may inhibit inflammation and cell pyroptosis in severe pneumonia rats by blocking the TXNIP/NLRP3 pathway.
2024 Vol. 30 (8): 1279-1284 [
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1284
Effect of Urolithin A on Malignant Biological Behavior of Triple-Negative Breast Cancer Cells via AKT/mTOR Signaling Pathway
A Jigu, HAN Jizhen, CHEN Yanyan, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.010
Objective:
To investigate the effect of urolithin A (UA) on the malignant biological behavior of triple-negative breast cancer (TNBC) cells and its regulatory mechanism on the protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway.
Methods:
The safety and toxicity of UA were assessed on human normal breast epithelial cell line MCF-10A and TNBC cell line MDA-MB-231 using the CCK-8 assay. MDA-MB-231 cells were randomly divided into the following groups: MDA-MB-231, MDA-MB-231 + UA, MDA-MB-231 + AKT activator (SC79), and MDA-MB-231 + UA + AKT activator (UA + SC79). The proliferation activity of each group was detected by colony formation assay. Cell migration and invasion abilities were examined by wound healing and Transwell assays. Apoptosis was assessed by flow cytometry. The activation levels of AKT/mTOR signaling pathway-related proteins were detected by Western blot.
Results:
UA at a concentration of 7.5μmoL/L was determined to be safe for normal breast cells and toxic to breast cancer cells. Compared to the MDA-MB-231 group, the UA group showed decreased colony formation (105.67±9.18), cell migration rate (12.92±2.13), invasion cell count (56.33±3.68), and p-AKT/AKT (0.12±0.02) and p-mTOR/mTOR (0.16±0.03) levels (P<0.05), while the apoptosis rate (22.1±1.75) increased (P<0.05). In contrast, the SC79 group showed increased colony formation (222±17.57), cell migration rate (62.7±6.14), invasion cell count (233.33±15.43), and p-AKT/AKT (0.58±0.07) and p-mTOR/mTOR (0.74±0.08) levels (P<0.05), and decreased apoptosis rate (4.57±0.57) (P<0.05). The addition of SC79 reversed the inhibitory effects of UA on the malignant biological behavior of MDA-MB-231 cells (P<0.05).
Conclusion:
UA can inhibit the proliferation, migration, invasion, and anti-apoptotic activities of TNBC cells by suppressing the AKT/mTOR signaling pathway.
2024 Vol. 30 (8): 1284-1290 [
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1290
Effect of LncRNA OIP5-AS1 on Proliferation of Neuroblastoma by Regulating the miR-186-5p/KIF14 Signaling Axis
AN Yanxiao, JIAO Hanliang, QI Yanwei, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.011
Objective:
To determine the role of long non-coding RNA (LncRNA) Opa-interacting protein 5 antisense RNA 1 (OIP5-AS1) in NB and its potential molecular mechanisms.
Methods:
Neuroblastoma cell lines SK-N-SH and human neuroblastoma BE2C were cultured in vitro. The expression of OIP5-AS1, miR-186-5p, and kinesin family member 14 (KIF14) was measured by quantitative real-time PCR. Cell proliferation was assessed using the CCK-8 assay, colony formation assay, and EdU incorporation assay. KIF14 protein levels were detected by Western blot. Target genes were predicted using the online database Starbase3.0 and verified by dual-luciferase reporter assay.
Results:
The expression level of OIP5-AS1 in SK-N-SH cells was significantly higher than that in BE2C cells (1.88±0.14 vs 1.00±0.07, P<0.05). After downregulating OIP5-AS1 expression in the shOIP5-AS1 group, SK-N-SH cell viability, colony formation ability (111.33±15.57 vs 154.67±20.74 vs 149.33±17.01, P<0.05), and DNA synthesis ability (EdU positive cell ratio: 35.09±8.02% vs 67.87±7.21% vs 63.33±7.17%, P<0.05) were weaker than those in the NC group and shCtrl group. Bioinformatics prediction and dual-luciferase reporter assay confirmed the targeting regulation between OIP5-AS1 and miR-186-5p as well as miR-186-5p and KIF14 3'-UTR. Compared with the NC group and shCtrl group, the shOIP5-AS1 group showed downregulated KIF14 mRNA relative expression (0.41±0.09, P<0.05) and KIF14 protein expression (0.20±0.02, P<0.05). Conversely, the shOIP5-AS1+miR-186-5p inhibitor group exhibited increased KIF14 mRNA relative expression and KIF14 protein expression (0.83±0.12 and 0.62±0.04, respectively) compared to the shOIP5-AS1+miR-NC group. RNA-binding protein immunoprecipitation results also showed that, compared with the control (IgG), the Ago2 antibody could enrich OIP5-AS1 and miR-186-5p. Compared with the shOIP5-AS1+miR-NC group, the shOIP5-AS1+miR-186-5p inhibitor group had significantly increased cell viability and colony formation ability (180.0±11.36 vs 136.0±14.53, P<0.05). In rescue experiments, compared with the shOIP5-AS1+miR-186-5p inhibitor+siNC group, the shOIP5-AS1+miR-186-5p inhibitor+siKIF14 group showed significantly decreased cell viability and colony formation ability (139.33±8.96 vs183.03±18.0, P<0.05).
Conclusion:
The expression level of OIP5-AS1 is significantly upregulated in SK-N-SH cells and acts as a competing endogenous RNA (ceRNA) to competitively inhibit miR-186-5p, thereby upregulating KIF14 expression and promoting NB progression.
2024 Vol. 30 (8): 1290-1296 [
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1296
Causal Relationship between Immune Cell Phenotypes and HSP27 Using Mendelian Randomization Method
CHEN Xi, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.012
Objective:
To investigate the causal effects of immune cell phenotypes on HSP27.
Methods:
This study utilized a two-sample Mendelian randomization (MR) analysis to determine the causal relationship between immune cell phenotypes and HSP27 expression levels. Based on publicly available genetic data, we explored the causal relationships between 731 immune cell phenotypes and HSP27 expression, encompassing four types of immune traits: median fluorescence intensity (MFI), relative cells (RC), absolute cells (AC), and morphological parameters (MP). Comprehensive sensitivity analyses were used to validate the robustness, heterogeneity, and horizontal pleiotropy of the results.
Results:
Bidirectional FDR correction showed that HSP27 expression did not significantly affect immune cell phenotypes (P>0.05). However, when examining the causal impact of immune cell phenotypes on HSP27 expression, we found causal relationships in three categories of immune traits (MFI, RC, AC) involving 21 immune phenotypes (P<0.05). Among them, 12 immune phenotypes promoted HSP27 expression (IVW:P<0.05, OR<1), including: IgD-CD24-AC; IgD-CD24-%lymphocyte; Myeloid DC AC; CD62L-myeloid DC AC; Activated Treg %CD4 Treg; CD38 on IgD+ CD38dim; CCR2 on granulocyte; CD80 on CD62L+ myeloid DC; CD80 on monocyte; CD8 on TD CD8br; CD4 on activated & secreting Treg; CD11c on granulocyte. Additionally,9 immune phenotypes inhibited HSP27 expression (IVW:P<0.05, OR>1), namely: CD62L-plasmacytoid DC AC; Naive CD4+ AC; CD14+ CD16+ monocyte AC; CD3 on T cell; CD3 on CD8br; HVEM on CM CD4+; HVEM on naive CD4+; CX3CR1 on CD14-CD16-; CD45 on Mo MDSC.
Conclusion:
This study reveals a significant genetic correlation between immune cell phenotypes and HSP27, which is important for understanding the pathological mechanisms of HSP27. It provides new insights for the future diagnosis and treatment of clinical diseases and helps develop more accurate biomarkers and therapeutic approaches. Moreover, our findings expand the scope of immunological research and offer new evidence for further studies on the interactions between immune cells and heat shock proteins in immune responses and diseases.
2024 Vol. 30 (8): 1296-1303 [
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1304
Analysis of CT Segmental Texture Characteristics and Relationship with Pulmonary Function Indices in COPD Patients
WANG Caicai, WANG Yuan, LI Jiangtao, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.013
Objective:
To analyze the CT segmented texture characteristics and the relationship with pulmonary function indicators in patients with chronic obstructive pulmonary disease (COPD).
Methods:
From March 2022 to March 2023, 80 patients with COPD were selected as the study subjects (study group), and 60 healthy people who received physical examination in the hospital were included in the control group. All patients underwent high-resolution CT scans and CT segmented texture characteristics and pulmonary function tests. The pulmonary function indicators [forced expiratory volume in the first second as a percentage of predicted value FEV1%), forced vital capacity (FVC), FEV1/FVC] and texture characteristics of the whole lung region and the outer lung region were compared between the two groups The correlation between CT texture characteristics and pulmonary function indicators was analyzed.
Results:
There were no statistical differences in age and gender between groups (P>0.05). The FEV1%, FVC, and FEV1/FVC in the study group were lower than those in the control group (P<0.05). The whole lung GLCM contrast, whole lung GLCM entropy, whole lung GLSZM area size non-uniformity, outer lung GLCM contrast, outer lung GLCM entropy, outer lung GLSZM area size non-uniformity, and outer lung LBP eigenvalue in the study group were higher than those in the control group (P<0.05). Pearson correlation analysis showed that whole lung GLCM contrast, whole lung GLCM entropy, whole lung GLSZM area size non-uniformity, outer lung GLCM contrast, outer lung GLCM entropy, outer lung GLSZM area size non-uniformity, and outer lung LBP eigenvalue were negatively correlated with FEV1%, FVC and FEV1/FVC (P<0.05). Multivariate regression analysis showed that whole lung GLCM contrast, whole lung GLCM entropy, whole lung GLSZM area size non-uniformity, outer lung GLCM contrast, outer lung GLCM entropy, outer lung GLSZM area size non-uniformity, and outer lung LBP eigenvalue had significant effects on FEV1%, FVC and FEV1/FVC in patients with COPD (P<0.05), and the R2 values were 0.682, 0.657 and 0.715 respectively, suggesting that the model had high goodness of fit.
Conclusion:
CT segmented texture characteristics are closely related to pulmonary function indicators of COPD patients. The GLCM contrast, GLCM entropy, GLSZM area size non-uniformity, and LBP eigenvalue of the whole lung and outer lung can be used as important reference indicators for imaging evaluation and early diagnosis of COPD.
2024 Vol. 30 (8): 1304-1309 [
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1309
Effect of Tiotropium Bromide Combined with Budesonide Inhalation Therapy on Lung Function and Sleep Quality in Patients with Asthma-Colonial Overlap Syndrome
WANG Baolan, WANG Liang, MA Ting
DOI: 10.3969/j.issn.1006-6233.2024.08.014
Objective:
To evaluate the effect of tiotropium bromide combined with budesonide inhalation therapy on lung function and sleep quality in patients with asthma-chronic obstructive pulmonary disease overlap syndrome (ACOS).
Methods:
A total of 98 ACOS patients treated in our hospital from January 2019 to January 2024 were sampled. They were divided into two groups using the random number table method, with 49 cases in each group. The control group was treated with budesonide inhalation, and the observation group was treated with tiotropium bromide based on the control group. Both groups were treated for 3 months. The two groups of patients were compared before and after treatment in terms of inflammation levels [interleukin 6 (IL - 6), tumor necrosis factor alpha (TNF alpha), hypersensitive c-reactive protein (hs - CRP)], immune function [17 (Th17 and regulatory T helper T cells , of Th17 / Treg cells (Treg)], airway remodeling factor [transforming growth factor beta 1 - (TGF β1) and osteopontin (OPN) and matrix metalloproteinases 9 (MMP 9)], lung function (forced expiratory volume in 1 second (FEV1) and forced vital capacity (FVC), and the largest independent ventilation per minute (MVV). Chronic obstructive pulmonary disease (CAT) score and asthma symptom score (ACT) were used to evaluate the disease symptoms before and after treatment, and Pittsburgh Sleep Quality Index (PSQI) scale was used to compare the sleep quality of the two groups after 3 months of treatment.
Results:
Before treatment, there were no significant differences in serum inflammatory factors, Th17, Th17/Treg, airway remodeling factors, lung function, CAT, and PSQI scores between the two groups (P>0.05). Compared with before treatment, the serum IL-6, TNF-α, hs-CRP, Th17, Th17/Treg, TGF-β1, OPN, MMP-9 levels, ACT and CAT scores in both groups were decreased after treatment, while Treg level, lung function FEV1, FVC, and MVV levels were increased. The difference was statistically significant(P<0.05). There were statistically significant differences in serum inflammatory factors, Th17, Th17/Treg, airway remodeling factors, lung function, CAT, and PSQI scores before and after treatment between the two groups (P<0.05). The sleep status of the observation group after treatment was better than that of the control group, and the difference was statistically significant (P<0.05).
Conclusion:
The combination of tiotropium and budesonide in the treatment of ACOS helps improve lung function and sleep quality.
2024 Vol. 30 (8): 1309-1315 [
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1316
Changes in Serum Cysc SAA Levels and Their Correlation with Cognitive Impairment in Parkinson's Disease Patients
LIN Baoping, ZHU Zuoquan, GUI Xin, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.015
Objective:
To analyze the changes in levels of serum cystatin C (CysC) and SAA in patients with Parkinson’s disease (PD) and their relationship with cognitive impairment.
Methods:
Patients who received treatment and physical examination in the hospital from August 2019 to August 2023 were selected as the research subjects, including 150 PD patients as the case group and 90 healthy volunteers as the control group. Cognitive function was assessed using Montreal Cognitive Assessment Scale (MoCA). The general data, serum CysC and SAA levels, and MoCA scores were compared. The levels of CysC and SAA were compared among patients with different Hoehn and Yahr (H-Y) stages. The CysC and SAA levels were compared between the case group and the control group. The correlation of CysC and SAA with H-Y staging and MoCA score was analyzed using Pearson analysis.
Results:
The levels of CysC and SAA in the case group were higher while the MoCA score was lower than in the control group (P<0.05). CysC and SAA revealed the control group < the early PD group < the middle and late PD group, while MoCA score revealed the control group > the early PD group > the middle and late PD group (P<0.05). CysC and SAA levels revealed the control group < normal cognition group < cognitive impairment group (P<0.05). The levels of serum CysC and SAA in PD patients were positively correlated with H-Y staging (r=0.610, 0.565, P<0.05), and were negatively associated with MoCA score (r=-0.352, -0.533, P<0.05).
Conclusion:
The levels of CysC and SAA in the serum of PD patients are increased. With the increase of H-Y stage, the levels of CysC and SAA rose while the MoCA score decreased. The levels of serum CysC and SAA in PD patients were positively correlated with H-Y staging and negatively correlated with MoCA score.
2024 Vol. 30 (8): 1316-1319 [
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1319
Impact of Cardiopulmonary Exercise Testing-Guided Cardiac Rehabilitation on Cardiorespiratory Function Exercise Tolerance and Quality of Life in Elderly Patients with CHD and HFpEF
ZHANG Yunshan, QIAN Jiali, CHEN Xiuli
DOI: 10.3969/j.issn.1006-6233.2024.08.016
Objective:
To explore the effects of cardiopulmonary exercise testing (CPET)-guided cardiac rehabilitation on cardiorespiratory function, exercise tolerance, and quality of life in elderly patients with coronary heart disease (CHD) and heart failure with preserved ejection fraction (HFpEF).
Methods:
A retrospective analysis was conducted on the clinical data of 103 elderly patients with coronary heart disease and HFpEF admitted to Kunming Second People's Hospital from June 2020 to May 2022. Patients were divided into two groups: the control group (51 cases) and the observation group (52 cases). The control group received conventional symptomatic treatment, while the observation group received CPET-guided cardiac rehabilitation in addition to the conventional treatment for 12 weeks. Changes in cardiorespiratory function [anaerobic threshold (AT), peak oxygen consumption (VO
2
peak), exercise duration (ED), and ventilatory equivalent for carbon dioxide slope (VE/VCO
2
slope)], myocardial enzyme levels [creatine kinase-MB (CK-MB), creatine kinase (CK), and lactate dehydrogenase (LDH)], N-terminal pro-brain natriuretic peptide (NT-proBNP), exercise tolerance [6-minute walk test (6 MWT)], Minnesota Living with Heart Failure Questionnaire (LHFQ) scores, and prognosis (1-year readmission rate and 1-year mortality rate) were compared before and after treatment.
Results:
After treatment, the observation group showed significantly higher AT 14.65±2.03mL·kg
-1
·min
-1
, VO
2
peak mL·kg
-1
·min
-1
, and ED [(436.89±46.85) s] compared to the control group (11.24±1.95)mL·kg
-1
·min
-1
,(21.65±2.49)mL·kg
-1
·min
-1
, and (378.46±46.18)s, respectively. The VE/VCO
2
slope in the observation group (30.27±2.75)mmoL/L was lower than in the control group (35.05±2.80)mmoL/L, with statistically significant differences (P<0.05). The observation group also had lower levels of CK-MB (136.84±24.35) IU/L, CK (28.48±4.29)IU/L, LDH (26.48±5.85)IU/L, and NT-proBNP (1535.25±32.09)pg/mL compared to the control group (325.45±35.48)IU/L, (56.26±5.84) IU/L, (49.32±6.88)IU/L, and (1716.73±39.42)pg/mL respectively, with statistically significant differences (P<0.05). The 6 MWT in the observation group [(405.46±52.28) m] was greater than in the control group [(345.19±48.64) m], with a statistically significant difference (P<0.05). LHFQ scores for emotional, physical, and other domains, as well as the total score, were significantly lower in the observation group [(8.26±1.95) points, (22.49±3.68) points, (21.58±3.39) points, and (56.29±5.39) points] compared to the control group [(11.38±2.26) points, (25.55±3.80) points, (24.68±3.97) points, and (63.45±6.98) points], with statistically significant differences (P<0.05). The 1-year readmission rate and 1-year mortality rate in the observation group were 15.38% and 5.77%, respectively, compared to 31.37% and 15.69% in the control group, with no statistically significant difference (P>0.05).
Conclusion:
CPET-guided cardiac rehabilitation can suppress myocardial enzyme levels, improve cardiorespiratory function, and enhance exercise tolerance and quality of life in elderly patients with coronary heart disease and HFpEF.
2024 Vol. 30 (8): 1319-1326 [
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1326
Analysis of Influencing Factors and Prediction Model for Patients with Acute Pancreatitis Complicated by Abdominal Infection
GE Chunyang, CAI Zhaohui, ZUO Shuang, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.017
Objective:
To analyze the influencing factors of patients with acute pancreatitis (AP) complicated by abdominal infection and to construct a prediction model, providing a reference for early prevention and treatment of AP with abdominal infection.
Methods:
Data from 103 AP patients admitted to Hefei Second People's Hospital between January 2021 and December 2023 were collected. Patients were divided into infected and non-infected groups based on the presence of abdominal infection. Independent influencing factors for AP with abdominal infection were identified, and a prediction model was constructed. The predictive value of the model for abdominal infection was assessed using ROC curve analysis.
Results:
Among the 103 AP patients, 32.04% (33/103) had abdominal infections. The predominant pathogens were Gram-negative bacteria, with Escherichia coli and Klebsiella pneumoniae showing high resistance to common antibiotics. Logistic regression identified age, length of hospital stay, APACHE II score at admission, CRP, PCT, and total NK cell count as independent influencing factors for AP with abdominal infection (P<0.05). ROC curve analysis showed the model's AUC was 0.843 (95% CI0.792~0.896), with a sensitivity of 61.36%, specificity of 92.06%, Youden index of 0.534, Homser-Lemeshow test P value of 0.267, and concordance rate of 96.12%.
Conclusion:
The risk of abdominal infection in AP patients is relatively high and is associated with age, length of hospital stay, APACHE II score at admission, CRP, PCT, and total NK cell count. The logistic prediction model effectively predicts the risk of abdominal infection in AP patients.
2024 Vol. 30 (8): 1326-1330 [
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1331
Correlation Analysis of the Expression Levels of Transmembrane Protein Klotho and Fibronectin in Keloids and Their Relationship with Postoperative Recurrence
SUN Yanping, LIU Pengchao, HU Jinhong, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.018
Objective:
To investigate the correlation between Klotho protein binding to fibronectin Fn and postoperative recurrence in keloids.
Methods:
A total of 43 patients with ear keloids and 37 general cosmetic seekers who visited our department from April 2020 to April 2023 were selected. The subjects were divided into a study group (keloid tissues and serum) and a control group (normal tissues and serum). Immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) were used to detect and compare the expression of Klotho protein and the epithelial-mesenchymal transition marker fibronectin FN in tissue and serum samples from both groups. Keloid patients were followed up at 1 month, 3 months, 6 months, and 12 months postoperatively to record recurrence, which divided them into recurrence and non-recurrence groups. Statistical methods were used to analyze the correlation between Klotho expression and fibronectin FN expression and postoperative recurrence in keloids.
Results:
Immunohistochemistry results showed that the positive rate of Klotho in the study group was lower than in the control group (P<0.05), at 37.20%. The positive rate of FN in the study group was 72.10%, higher than in the control group (P<0.05). The positive rate of Klotho in the recurrence group was 21.40%, lower than in the non-recurrence group (P<0.01), and the positive rate of FN in the recurrence group was 92.90%, higher than in the non-recurrence group (P<0.05). There was a statistically significant correlation between the levels of Klotho and FN proteins (r=-0.307, P<0.05). Elisa results showed that the expression level of Klotho in the study group was 65.99 (55.05,79.06) ng/mL, lower than in the control group (P<0.05), and the expression level of FN in the study group was 83.56 (62.67,91.85) ng/mL, higher than in the control group (P<0.01). Logrank tests indicated that negative Klotho expression and positive FN protein expression were associated with postoperative recurrence (both P<0.05).
Conclusion:
The combination of Klotho protein and the epithelial-mesenchymal transition marker FN can be used as an important marker to predict postoperative recurrence of keloids.
2024 Vol. 30 (8): 1331-1336 [
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1336
Correlation of Th1/Th2 Balance and HLA-DQA1 Gene Polymorphism with Hemodialysis Catheter-Related Infections
HE Fan, YANG Wenjun, MA Li, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.019
Objective:
To explore the correlation between Th1/Th2 balance, human leukocyte antigen DQA1 (HLA-DQA1) gene polymorphism, and catheter-related infections during hemodialysis.
Methods:
Forty-eight patients with hemodialysis catheter-related infections treated at our hospital from January 2020 to January 2024 were selected as the observation group. Two hundred sixty patients undergoing hemodialysis without catheter-related infections during the same period were selected as the control group. The differences in Th1 cells, Th2 cells, associated cytokines, and HLA-DQA1 gene polymorphisms between the two groups were compared.
Results:
The Th1, Th2, TNF-α, TGF-1, IL-4, and IL-6 levels in the observation group were (43.35±9.10)%, (3.10±0.92)%, (46.22±8.87) pg/mL, (50.05±13.36) pg/mL, (1.03±0.35) pg/mL, and (40.05±11.65) pg/mL, respectively, higher than those in the control group (P<0.05), while Th1/Th2 levels were (14.01±2.20), lower than those in the control group (P<0.05). There was a statistically significant difference in HLA-DQA1 genotype between the observation group and the control group (P<0.05). The TNF-α, IL-4, and IL-6 of the observation group's deceased patients were (58.07±2.65) pg/mL, (1.22±0.21) pg/mL, and (46.67±9.96) ng/L, respectively, higher than those of the surviving patients (P<0.05). There was no statistically significant difference in HLA-DQA1 gene polymorphism between surviving and deceased patients in the observation group (P>0.05).
Conclusion:
Patients with hemodialysis catheter-related infections exhibit elevated levels of Th1 cells, Th2 cells, and associated factors. There are differences in HLA-DQA1 genotypes between patients with and without infections, with TNF-α, IL-4, and IL-6 levels associated with prognosis.
2024 Vol. 30 (8): 1336-1340 [
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1340
Study on the Role of Modified Platelet-Rich Fibrin in Socket Preservation for Preservation of Alveolar Bone Quantity and Maintenance of Alveolar Bone Morphology
YANG Yi, MA Yuanjun, CAI Wei, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.020
Objective:
To analyze the role of modified platelet-rich fibrin (PRF) in socket preservation for the preservation of alveolar bone quantity and maintenance of alveolar bone morphology.
Methods:
A total of 105 patients undergoing socket preservation surgery from September 2021 to September 2023 were randomly divided into an observation group (n=52, treated with modified PRF) and a control group (n=53, conventional treatment). Clinical treatment outcomes, changes in alveolar bone quantity, and alveolar bone morphology before and after treatment were compared between the two groups.
Results:
Compared with the control group, the observation group had a shorter healing time (P<0.05), and higher wound healing rates at 1 week and 2 weeks postoperatively (P<0.05). At 4 months postoperatively, there were no significant differences in bone crest level (BC1) between the two groups (P>0.05), but the observation group had higher mean gray value (BD2) and bone gain (BG) compared to the control group (P<0.05), while BC2 was lower in the observation group (P<0.05). At 6 months postoperatively, the observation group showed greater improvements in alveolar bone width, height, density levels, and improvement rates compared to the control group (P<0.05). At 3 months postoperatively, the sulcus bleeding index (SBI) was lower in the observation group than in the control group (P<0.05).
Conclusion:
Modified PRF used in socket preservation surgery demonstrates good osteogenic effects, aids in wound healing, and plays a beneficial role in preserving alveolar bone quantity and maintaining alveolar bone morphology.
2024 Vol. 30 (8): 1340-1344 [
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1344
Effectiveness of Microscopic Root Canal Treatment Based on Periodontal Microbial Ecology Assessment in the Treatment of Radicular Groove-Associated Pulpal-Periodontal Lesions
ZHANG Na, WANG Ning, FANG Ling, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.021
Objective:
To evaluate the effectiveness of microscopic root canal treatment based on periodontal microbial ecology assessment in the treatment of radicular groove-associated pulpal-periodontal lesions.
Methods:
A total of 118 patients diagnosed with radicular groove-associated pulpal-periodontal lesions from October 2020 to October 2023 were randomly divided into two groups: the control group (n=59) treated with periodontal tissue regeneration surgery, and the observation group (n=59) treated with microscopic root canal treatment combined with periodontal tissue regeneration surgery. The distribution of periodontal microbiota, gingival index (GI), sulcus bleeding index (SBI), plaque index (PLI), tooth mobility (TM), as well as treatment efficacy and safety were compared between the two groups before and after surgery.
Results:
At 1 week postoperatively, the observation group showed lower detection rates of Streptococcus mutans, Porphyromonas gingivalis, and Fusobacterium nucleatum in periodontal pockets compared to the control group (P<0.05). The observation group also exhibited lower GI, SBI, PLI, and TM compared to the control group at 1 week postoperatively (P<0.05). The clinical efficacy in the observation group was superior to that in the control group, and both groups did not experience significant complications.
Conclusion:
Microscopic root canal treatment for radicular groove-associated pulpal-periodontal lesions demonstrates significant effectiveness, effectively improves periodontal microbiota and microbial ecology, and reduces tooth mobility.
2024 Vol. 30 (8): 1344-1349 [
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1349
Application Study of bFGF Combined with Ultrasonic Bone Scalpel in Removal of Impacted Mandibular Teeth
WEI Endian, FU Xiao, YOU Jingxiu
DOI: 10.3969/j.issn.1006-6233.2024.08.022
Objective:
To explore the application effectiveness of recombinant bovine basic fibroblast growth factor (bFGF) combined with ultrasonic bone scalpel in the removal of impacted mandibular teeth.
Methods:
A total of 105 patients with impacted mandibular teeth treated at our hospital from October 2020 to October 2023 were randomly divided into a control group (n=52) and an observation group (n=53) using simple random grouping. The control group underwent bFGF combined with conventional rotary drilling for extraction, while the observation group underwent bFGF combined with ultrasonic bone scalpel for extraction. Surgical-related indicators, clinical treatment outcomes, postoperative swelling, restricted mouth opening recovery, expression levels of inflammatory factors [tumor necrosis factor-alpha (TNF-α), intercellular adhesion molecule 1 (ICAM1), myeloperoxidase (MPO)] in gingival crevicular fluid, and dry socket occurrence were compared between the two groups.
Results:
The operation time and intraoperative blood loss in the observation group were significantly lower than those in the control group (P<0.05). Before and after surgery, the improvement in systolic blood pressure, diastolic blood pressure, and blood glucose levels in the observation group was lower than that in the control group (P<0.05). The excellent and good treatment rate in the observation group was 98.11%, which was higher than 82.69% in the control group (P<0.05). Postoperative swelling and incidence of restricted mouth opening were lower in the observation group compared to the control group (P<0.05). The increase in TNF-α, ICAM1, and MPO levels in the gingival crevicular fluid was lower in the observation group than in the control group (P<0.05). The total incidence of complications in the observation group was lower than that in the control group (P<0.05).
Conclusion:
bFGF combined with ultrasonic bone scalpel for the treatment of impacted mandibular teeth is effective, improves inflammatory responses in gingival crevicular fluid, and reduces the risk of complications.
2024 Vol. 30 (8): 1349-1353 [
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1354
Diagnostic Value and Imaging Features of Retinoblastoma (Rb) on MSCT and MRI
JIANG Shijie, WANG Li, ZHANG Hui, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.023
Objective:
To compare the diagnostic value of MSCT and MRI in the detection of retinoblastoma (Rb).
Methods:
A total of 125 suspected Rb patients treated at our hospital from January 2019 to June 2023 were included. MSCT and MRI were performed, with pathological findings used as the gold standard for diagnosis. Sensitivity, specificity, accuracy, and consistency with pathological results were analyzed for both imaging modalities.
Results:
The positivity rates of Rb detected by MRI, MSCT, and pathology were 44.00% (55/125), 52.80% (66/125), and 45.60% (57/125), respectively, with no statistically significant difference (P>0.05). The Kappa value for MRI compared to pathology was 0.644, lower than the Kappa value of 0.729 for MSCT. The sensitivity of MRI (78.94%) was lower than that of MSCT (92.98%), with a statistically significant difference (P<0.05). The specificity of MRI (85.29%) was higher than that of MSCT (80.88%), while the accuracy of MRI (82.40%) was lower than that of MSCT (86.40%), but these differences were not statistically significant (P>0.05). MSCT showed irregular thickening of the eyeball wall, irregular nodular soft tissue density in the vitreous cavity, and patchy calcifications within the lesion. MRI revealed uneven thickening of the eyeball ring, irregular mass-like abnormal signal shadows in the vitreous cavity, iso-intense signal on T1-weighted imaging (T1WI), slightly hyper-to hypo-intense mixed signal on T2-weighted imaging (T2WI), and heterogeneous enhancement on contrast-enhanced scans.
Conclusion:
Compared to MRI, MSCT demonstrates higher consistency with pathological findings and higher sensitivity in detecting Rb, indicating superior diagnostic value.
2024 Vol. 30 (8): 1354-1357 [
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1358
MRI Imaging Features and Diagnostic Value in Patients with Cruciate Ligament Injuries of the Knee
DAI Chengzhong, WANG Haodong
DOI: 10.3969/j.issn.1006-6233.2024.08.024
Objective:
To observe imaging features of MRI in patients with anterior cruciate ligament (ACL) injury of knee joint, and to analyze their diagnostic value.
Methods:
This study included 109 patients with knee joint injuries treated at our hospital from March 2020 to December 2023. All patients underwent MRI and arthroscopy, with arthroscopy serving as the "gold standard." Patients were divided into an ACL group and a non-ACL group. The MRI imaging features (direct and indirect signs) of both groups were compared. Kappa consistency analysis was used to evaluate the diagnostic value of each imaging feature for ACL injuries, and sensitivity and specificity were calculated.
Results:
Arthroscopy revealed 46 cases of ACL injuries, including 32 complete tears, 9 partial tears, and 5 tibial avulsions. There were significant differences between the ACL group and the non-ACL group in three direct signs (ACL discontinuity, abnormal course, and edema and thickening) and ten indirect signs (Blumensaat angle, PCL angle, PCL index, bone contusion, notch sign, anterior tibial displacement, uncovered posterior horn of the meniscus, empty intercondylar notch sign, single-layer display of the lateral collateral ligament, and single-layer display of the PCL) (P<0.05). Kappa consistency analysis showed that among the direct signs, the abnormal course had the highest Kappa consistency (Kappa=0.790, P<0.001), and among the indirect signs, Blumensaat angle had the highest Kappa consistency (Kappa=0.569, P<0.001). Sensitivity was highest for the direct sign of abnormal course, while specificity was highest for the indirect sign of empty intercondylar notch sign (P<0.05).
Conclusion:
MRI imaging features of ACL injuries are distinct, with primary signs being edema and thickening, and abnormal course. The indirect sign of Blumensaat angle can serve as a primary diagnostic basis, while the indirect signs of empty intercondylar notch sign, notch sign, and single-layer display of the PCL can serve as key auxiliary indicators.
2024 Vol. 30 (8): 1358-1362 [
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1362
Efficacy of rTMS Combined with Pramipexole in Elderly Patients with Parkinson's Disease and Impact on Gait Parameters and Serum Levels of S100β NSE BDNF
SHI Jie, GENG Xiaokang, LI Xiao, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.025
Objective:
To explore the efficacy of repetitive transcranial magnetic stimulation (rTMS) combined with pramipexole in the treatment of elderly patients with Parkinson' s disease (PD) and its impact on gait indicators, serum central nervous specific protein (S100β), neuron-specific enolase (NSE), and brain-derived neurotrophic factor (BDNF).
Methods:
A total of 102 elderly inpatients diagnosed with PD from May 2020 to May 2023 were included in this study. Patients were divided into medication group (single) and combined treatment group (double) using odd and even numbers, with 51 patients in each group. Patients in the medication group received pramipexole therapy starting at 0.125 mg three times daily, increasing weekly up to a maximum of 4.5 mg/day. Patients in the combined treatment group received rTMS in addition to pramipexole, with one session per day for 5 days followed by 2 days of rest, for a total of 8 weeks of treatment. Clinical efficacy, emotional function, gait parameters, cortical excitability indices, serum biochemical parameters, and adverse reactions were compared between the two groups.
Results:
The total effective rate in the combined treatment group (86.27%) was higher than that in the medication group (68.63%) (P<0.05). After treatment, both groups showed significant decreases in HAMD and FAQ scores (P<0.05). Post-treatment, STEF and BBS scores increased while MAS scores decreased in both groups, with MAS scores lower in the combined treatment group (P<0.05). MEP decreased, and RMT and CMCT increased significantly post-treatment in both groups (P<0.05). Post-treatment, S100β and NSE levels decreased while BDNF levels increased significantly in both groups (P<0.05). There was no significant difference in the incidence of adverse reactions between the combined treatment group (9.80%) and the medication group (7.84%) (P>0.05).
Conclusion:
rTMS combined with pramipexole effectively treats elderly patients with PD, improving emotional, motor, and neurological functions with a high safety profile. This combined approach warrants further promotion in clinical practice.
2024 Vol. 30 (8): 1362-1367 [
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1367
Comparative Study of Precise CT-Guided Percutaneous Vertebroplasty versus Traditional Percutaneous Vertebroplasty for Thoracolumbar Compression Fractures
LIAO Wei, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.026
Objective:
To retrospectively compare the clinical efficacy of percutaneous kyphoplasty (PKP) assisted by precise CT-guided puncture with traditional percutaneous methods in patients with osteoporotic vertebral compression fractures (OVCFs).
Methods:
A retrospective analysis was conducted on 56 patients who underwent PKP for single-segment OVCFs between January 2017 and June 2021. Patients were divided into two groups based on different methods of intraoperative puncture site localization: Group A (precise CT-guided puncture) and Group B (traditional method). Each group consisted of 28 patients. The number of puncture attempts, fluoroscopy times, and operation duration were compared between the two groups. Clinical efficacy was assessed using Visual Analogue Scale (VAS) scores preoperatively and postoperatively. Incidence of procedure-related complications was also monitored.
Results:
All 56 patients underwent successful puncture and were followed up for an average of 10 months (range: 8~16 months). In Group A (precise CT-guided), the mean operation time was 34.1±7.5 minutes, fluoroscopy times during surgery wer 9.9±2.5, and cement injection volume per fractured vertebra was 5.7±0.8mL. Preoperative VAS score was 8.2±0.8, which decreased to 1.4±0.6 at 6 months postoperatively. In Group B (traditional method), the mean operation time was 48.8±9.5 minutes, fluoroscopy times were 14.5±3.5, and cement injection volume per fractured vertebra was 5.6±0.6mL. Preoperative VAS score was 8.2±0.7, which decreased to 1.5±0.6 at 6 months postoperatively. There were no statistically significant differences between the two groups in terms of cement injection volume and VAS scores at 6 months postoperatively (P>0.05). However, significant differences were found in the number of puncture attempts, operation time, and fluoroscopy times during surgery (P<0.05). Group A achieved a significantly higher single-puncture success rate of 92.9% (26/28) compared to 53.6% (15/28) in Group B. Complications included cement leakage towards the posterior or lateral aspects of the vertebra in 2 cases in Group A and in 3 cases involving both anterior and lateral aspects in Group B. No neurological or spinal cord injury complications occurred in either group, and there were no wound infections.
Conclusion:
Precise CT-guided puncture based on preoperative PACS system CT measurements significantly improves the success rate of single-puncture procedures and enhances the accuracy of percutaneous techniques compared to traditional methods. This approach reduces the number of fluoroscopy sessions and operation time, offering a more precise, minimally invasive alternative for treating OVCFs. It is deemed a safe, reliable, and effective technique.
2024 Vol. 30 (8): 1367-1376 [
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1376
Effects of Pedicle 6-Screw Internal Fixation through the Injured Vertebra and Pedicle 4-Screw Internal Fixation across the Injured Vertebra on Spinal Stability in A1 Type Thoracolumbar Fractures
CHEN Bo, GUO Yifan, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.027
Objective:
To study the effects of different internal fixation operations on the spinal stability of A1 thoracolumbar fracture.
Methods:
A retrospective analysis was conducted on the clinical data of 146 patients with A1 type thoracolumbar fractures treated in our hospital from January 2021 to January 2023. Based on the method of internal fixation surgery, 82 patients who underwent six-screw transpedicular fixation were included in Group A, and 64 patients who underwent four-screw transpedicular fixation were included in Group B. Perioperative indicators, sagittal Cobb angle, vertebral height compression rate, Oswestry Disability Index (ODI), stress indicators, and postoperative complications were compared between the two groups.
Results:
No significant differences were found between Group A and Group B in terms of intraoperative blood loss (248.49±22.36 mL vs. 244.03±20.71 mL), bed rest duration (4.92±1.24 days vs. 5.03±1.10 days), and hospital stay length (8.35±1.76 days vs. 8.75±1.43 days) (P>0.05). The surgery time was significantly longer in Group A (129.80±17.02 minutes) compared to Group B (122.95±15.63 minutes) (P<0.05). At the T2 time point, the sagittal Cobb angle, vertebral height compression rate, and ODI were significantly lower in Group A than in Group B (P<0.05). There were no significant differences in the levels of prostaglandin E2 (PGE2) and norepinephrine (NE) between the two groups at 24 hours and 3 days postoperatively (P>0.05). The incidence of postoperative complications did not differ significantly between the two groups (χ
2
=2.598, P=0.107).
Conclusion:
Although the six-screw transpedicular fixation surgery has a longer duration, it more effectively restores spinal stability and improves functional outcomes in patients with A1 type thoracolumbar fractures.
2024 Vol. 30 (8): 1376-1381 [
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1381
Analysis of Risk Factors for Invasive Klebsiella Pneumoniae Liver Abscess Syndrome
ZHANG Tiezhao, CAO Xidong, LI Kefan, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.028
Objective:
To analyze the clinical characteristics and risk factors associated with invasive Klebsiella pneumoniae liver abscess syndrome (IKPLAS).
Methods:
A retrospective analysis was conducted on the clinical data of 442 patients with Klebsiella pneumoniae liver abscess admitted to the Affiliated Hospital of Chengde Medical University from August 1, 2014, to June 31, 2023. The patients were divided into the IKPLAS group (431 cases) and the non-IKPLAS group (11 cases). Clinical features were compared between the two groups. Univariate and multivariate logistic regression analyses were used to identify risk factors for IKPLAS in Klebsiella pneumoniae liver abscess patients.
Results:
Univariate analysis indicated that diabetes, perihepatic abscess, neutrophil ratio, hemoglobin level, gas-containing abscess, and SOFA score were associated with the development of IKPLAS in patients with Klebsiella pneumoniae liver abscess. Multivariate logistic regression analysis identified diabetes at admission [OR=8.922 (95%CI: 1.676~47.505)], neutrophil ratio, and hemoglobin level as independent risk factors for IKPLAS, with OR values of 0.947 (95%CI:0.903~0.997) and 0.970 (95% CI:0.949~0.997), respectively.
Conclusion:
Diabetes, decreased neutrophil ratio, and reduced hemoglobin are independent risk factors for the development of IKPLAS in patients with Klebsiella pneumoniae liver abscess.
2024 Vol. 30 (8): 1381-1386 [
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1386
Correlation Study of Platelet Parameters NF-κB IL-6 Levels with Disease Severity and Prognosis in Patients with Cirrhosis Complicated by Upper Gastrointestinal Bleeding
CAI Xu, XU Shuai, SHANG Shuai
DOI: 10.3969/j.issn.1006-6233.2024.08.029
Objective:
To analyze the correlation between platelet parameters, serum nuclear factor-κB (NF-κB), interleukin-6 (IL-6) levels, and the disease severity and prognosis in patients with cirrhosis complicated by upper gastrointestinal bleeding (UGIB).
Methods:
A total of 155 patients with cirrhosis complicated by UGIB, treated at our hospital from January 2021 to January 2024, were included in the study. Based on the amount of blood loss before admission, they were divided into a mild group (n=70) and a moderate to severe group (n=85). Additionally, based on the prognosis, they were categorized into a good prognosis group (n=122) and a poor prognosis group (n=33). Platelet parameters [platelet count (PLT), mean platelet volume (MPV), plateletcrit (PCT), platelet distribution width (PDW)], serum NF-κB, and IL-6 levels were measured in all patients. Spearman rank correlation analysis was used to evaluate the relationship between these parameters and the severity and prognosis of cirrhosis complicated by UGIB.
Results:
The PLT, MPV, PCT, PDW, serum NF-κB, and IL-6 levels in the moderate to severe group were significantly higher than those in the mild group (P<0.05). Similarly, these parameters were higher in the poor prognosis group compared to the good prognosis group (P<0.05). Spearman rank correlation analysis indicated a positive correlation between PLT, MPV, PCT, PDW, serum NF-κB, IL-6 levels, and the severity of cirrhosis complicated by UGIB (r=0.226,0.311,0.387,0.311,0.351,0.386; P<0.05).
Conclusion:
Platelet parameters, NF-κB, and IL-6 levels are closely related to the severity and prognosis of patients with cirrhosis complicated by UGIB. Early detection of these serum indicators can provide a reference for clinical assessment of disease severity and prognosis.
2024 Vol. 30 (8): 1386-1390 [
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Construction of a Predictive Model for Recurrent Embryo Implantation Failure in Patients with Unexplained Infertility Based on Vaginal Doppler Ultrasound and Clinical Data
FENG Lin, SI Fan, XU Lili, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.030
Objective:
To construct a predictive model for recurrent embryo implantation failure in unexplained infertility patients based on vaginal Doppler ultrasound examination results and clinical data, and to analyze the influencing factors.
Methods:
The study group consisted of 198 unexplained infertility patients with recurrent embryo implantation failure from June 2020 to December 2022 at our hospital. The control group included 100 infertility patients with successful embryo implantation during the same period. All patients underwent vaginal Doppler ultrasound examinations. The results of the ultrasound examinations and clinical data were compared between the two groups. Receiver Operating Characteristic (ROC) curve analysis was used to evaluate the predictive value of multiple factors on embryo implantation outcomes. A Logistic regression model was established to identify the influencing factors of recurrent embryo implantation failure and to validate the model.
Results:
The study group had a higher age, higher AMH levels, higher rate of abnormal autoantibodies, and more induced abortions compared to the control group (P<0.05). The study group had a thinner endometrial thickness on the day of transfer and a higher uterine artery pulsatility index compared to the control group (P<0.05). ROC curve analysis showed that age, AMH, number of induced abortions, autoantibodies, endometrial thickness on the day of transfer, and uterine artery pulsatility index had predictive value for embryo implantation outcomes in infertility patients (AUC=0.706, 0.720, 0.611, 0.649, 0.719, 0.771). The Logistic regression model showed that advanced age, a higher number of induced abortions, abnormal autoantibodies, and a higher uterine artery pulsatility index were risk factors for recurrent implantation failure in infertility patients (OR>1, P<0.05); higher AMH levels and greater endometrial thickness on the day of transfer were protective factors for embryo implantation outcomes (OR<1, P<0.05). The ROC curve drawn based on the Logistic regression model showed that the model's predictive value for recurrent implantation failure in infertility patients was AUC=0.928, P<0.05, indicating that the model was reliable and stable.
Conclusion:
Recurrent implantation failure in infertility patients may be related to factors such as age, AMH levels, number of induced abortions, autoantibodies, endometrial thickness on the day of transfer, and uterine artery pulsatility index. Clinicians can use transvaginal Doppler ultrasound examination before embryo implantation, combined with clinical data, to comprehensively evaluate the patient's embryo implantation outcomes and take targeted interventions to achieve a favorable pregnancy outcome.
2024 Vol. 30 (8): 1390-1396 [
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Comparative Study of Remifentanil Combined with Propofol versus Propofol Alone in Anesthesia for Hysteroscopic Electrosurgical Procedures
TONG Bin, WU Yue, HE Rui, et al
DOI: 10.3969/j.issn.1006-6233.2024.08.031
Objective:
To investigate the application efficacy of two total intravenous anesthesia regimens, remifentanil combined with alfentanil versus propofol combined with alfentanil, during hysteroscopic electrosurgical procedures.
Methods:
From January 2023 to June 2023, a total of 107 patients undergoing hysteroscopic electrosurgical procedures were included and randomly allocated using a random number table into a control group (n=53) receiving propofol combined with alfentanil anesthesia and an observation group (n=54) receiving remifentanil combined with alfentanil anesthesia. Pain intensity (evaluated using VAS scores), sedation levels (assessed by Ramsay Sedation Scale), vital signs, Modified Aldrete Score (MAS), and adverse reactions were compared between the two groups.
Results:
Compared with the control group, the observation group showed significantly lower VAS scores at 2 hours and 12 hours postoperatively (P<0.05) and higher Ramsay Sedation Scale scores at 2 hours postoperatively (P<0.05). There were no significant differences in preoperative heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP), and oxygen saturation (SPO
2
) between the two groups (P>0.05). However, HR, SBP, DBP, and SPO
2
levels were significantly higher in the observation group than in the control group at 5 minutes after the start of surgery and at 2 hours postoperatively (P<0.05). Significant group effects, time effects, and interaction effects were observed for VAS scores, Ramsay Sedation Scale scores, and vital signs (P<0.05). The observation group had higher Modified Observer's Assessment of Alertness/Sedation (MOAA/S) scores at 1, 3, and 5 minutes after surgery compared to the control group (P<0.05). There was no statistically significant difference in the incidence of adverse reactions between the two groups (P>0.05).
Conclusion:
Remifentanil combined with alfentanil provides better pain control and sedation during early postoperative period in patients undergoing hysteroscopic electrosurgical procedures compared to propofol combined with alfentanil. Patients recover faster to a clear consciousness state, and their vital signs are more stable during anesthesia.
2024 Vol. 30 (8): 1396-1401 [
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