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| The Role and Clinical Value of Th22 Cells in Peripheral in Rheumatoid Arthritis |
| TANG Yan, et al |
| Daping Hospital & Institute of Surgery Research, Army Medical University / The Third Military Medical University, Chongqing 400042, China |
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Abstract Objective: To study the role and clinical value of Th22 cells levels in peripheral blood in rheumatoid arthritis (RA). Methods: According to the DAS28 score, 190 patients with RA were divided into DAS28≤3.2 (52 cases), 3.2<DAS28≤5.1 (63 cases), DAS28>5.1 (75 cases), 60 healthy subjects were selected as the control group. The levels of Th22 cells in peripheral blood of each group were detected by flow cytometry. The levels of related cytokines (IL-22, IL-17) were detected by enzyme-linked immunosorbent assay (ELISA). The differences of Th22 cells in peripheral blood were compared. The predictive value of Th22 cell in peripheral blood was evaluated in the degree of activity in RA. Results: Compared with healthy controls, the levels of Th22 cells and cytokine IL-22 in peripheral blood in patients with RA were significantly increased (P<0.05); The levels of Th22 cells, IL-22 and IL-17 in peripheral blood in different activity degrees of RA were statistically signifiant (P<0.05). Among them, the level of the above indicators in the DAS28>5.1 group was the highest, the 3.2<DAS28≤5.1 group was the second, and the DAS28≤3.2 group was the lowest. The level of Th22 cells in peripheral blood in patients with RA group was positively correlated with IL-22, IL-17 and DAS28 (r=0.831, P=0.000; r=0.575, P=0.025; r=0.673, P=0.014). ROC curves analysis showed that the area under curve of Th22 cells was 0.874 (95%CI 0.801~0.948), and optimal operating point (OOP) was 8.5%, which had 84.38% sensiticity and 81.48% specificity in predicting the activity degree of RA patients. Conclusions: The level of Th22 cells in peripheral blood is closely related to the occurrence of development of RA patients, and there is certain application value for predicting the activity degree of RA.
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2019, Vol. 25 
