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| Research of renal cell carcinoma RASSF2A gene methylation and expression status |
| FAN Bo, WANG Zhongkai, QI Pan, et al |
| The Fourth Hospital of Hebei Medical University, Hebei Shijiazhuang 050000, China |
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Abstract Objective: To investigate the renal cell carcinoma RASSF2A gene methylation and expression status of research, and to analyze the occurrence of renal cell carcinoma development in rats. Methods: Methylation specific polymerase chain reaction (Methylation-Specific PCR, MSP) and reverse transcription - polymerase chain reaction (Reverse Transcriptase-PCR, RT-PCR) to detect 60 cases of renal cell carcinoma patients, paraneoplastic RASSF2A normal tissue gene expression relative situation promoter methylation status and mRNA. Results: In the 60 cases ,The methylation rate of cancer tissue was significantly higher than that of cancer paraneoplastic and normal tissues. The relative expression of RASSF2A gene mRNA was significantly lower than that of cancerous bypass and normal tissue. The difference was statistically significant (P<0.05). There was no significant difference between the methylation rate and the relative expression of mRNA (P>0.05); In the cancer tissue, the relative expression of mRNA in the methylation positive patients was lower than that of the mRNA in the negative methylation patients, and the difference was statistically significant(P<0.05). There was positive correlation between RASSF2A gene methylation and protein expression of β-catenin in renal cell carcinoma tissues(r=0.269,P<0.05). Conclusion: RASSF2A promoter hypermethylation is RCC frequent events, tumor suppressor gene RASSF2A inactivation may be one of the important reasons leading to transcriptional silencing, RASSF2A promoter hypermethylation and mRNA expression decrease may be involved in the RCC the occurrence and development.RASSF2A gene methylation might be related to the high expression of β-catenin gene. It had effect on the invasion and metastasis of renal cell carcinoma through changing the intracellular distribution of β-catenin. It indicated that this signaling pathway was involved in tumorigenesis.
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[1] 王仲凯.肾细胞癌RASSF2A基因启动子甲基化状态及转录表达的研究[D].河北医科大学,2016,1~46.
[2] 樊博,齐盼,张爱莉等.肾细胞癌中DACT2基因启动子区甲基化状态与mRNA表达的研究[J].重庆医学,2017,46(21):2895~2897,2901.
[3] Xia Y1, Xiong N2, Huang Y2 .Relationship between methylation status of RASSF2A gene promoter and endometriosis-associated ovarian cancer[J].Biol Regul Homeost Agents,2018,32(1):21~28.
[4] Kurkjian C, Kummar S, Murgo A J. DNA Methylation: Its Role in Cancer Development and Therapy[J]. Current Problems in Cancer, 2008, 32(5):187~235. |
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2019, Vol. 25 
