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| Study on the Correlation between Peripheral Blood MiR-223 and MiR-146a Levels and Acute Ischemic Stroke |
| YANG Yuanyuan, et al |
| The Fourth Affiliated Hospital of Harbin Medical University, Heilongjiang Harbin 150001, China |
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Abstract Objective: To investigate the expressions of miR-223 and miR-146a in peripheral blood of acute ischemic stroke and their relationships with the occurrence of disease. Methods: 76 cases of acute ischemic stroke diagnosed and treated in the Fourth Affiliated Hospital of Harbin Medical University from January 2017 to April 2018 were selected as the subjects (case group), according to the TOAST etiological classification, there were 33 cases of main artery atherosis type, 19 cases of cardiogenic embolism type, 16 cases of small artery occlusion type, and 8 cases of unknown cause type. 60 healthy subjects were as the control group. The expression levels of miR-223 and miR-146a in peripheral blood were detected by RT-PCR, NIHSS score was used to assess the severity of acute ischemic stroke, the relationships between miR-223 and miR-146a levels with acute stroke were analyzed. Results: The expression level of miR-223 in peripheral blood of case group was significantly higher than that of control group (P<0.05), the expression level of miR-146a was significantly lower than that of the control group (P<0.05); there was no significant difference in the expression levels of miR-223 and miR-146a between the different etiological subtypes (P>0.05); there was a significant positive correlation between miR-233 level with NIHSS score in case group (r=0.774, P=0.000), there was a significant negative correlation between miR-146a level with NIHSS score (r=0.741, P=0.000); hypertension, hyperglycemia, platelet count, INR and miR-223 were risk factors for acute ischemic stroke, miR-146a is a protective factor for acute ischemic stroke. Conclusion: The miR-223 in peripheral blood of patients with acute ischemic stroke is high expressed, the miR-146a is low expressed, it is closely related to the severity of the patient's condition
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2019, Vol. 25 
