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| Protective Effect of Angelica Polysaccharides on Energy Metabolism Disorder of H9c2 Myocardial Cells Induced by Hypoxia-reoxygenation Injury |
| HUO Lichao, LI Mengli, QIAO Chengdong |
| The First Hospital of Lanzhou University, Gansu Lanzhou 730010, China |
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Abstract Objective: To explore the protective effect of Angelica polysaccharide (ASP) on energy metabolism disorder of H9c2 myocardial cells induced by hypoxia-reoxygenation damage. Methods: MTT was used to detect the effect of polysaccharides from Angelica sinensis on the proliferation of H9c2 cells. The hypoxia-reoxygenation model of Angelica sinensis polysaccharide intervention and non-intervention was constructed in vitro; cell morphology was observed; apoptosis was detected by flow cytometry; mitochondrial membrane potential detection kit was used to detect cells. Mitochondrial membrane potential changes; kits were used to detect the activity of Na+-K+ATPase and Ca2+-Mg2+ATPase and mitochondrial respiratory enzyme chain complex I and IV in LDH and cardiomyocytes in culture supernatant of cardiomyocytes. Results: Angelica polysaccharide inhibited the proliferation of H9c2 cells in a concentration-dependent manner. Hypoxia-reoxygenation induced apoptosis of H9c2 cells, decreased mitochondrial membrane potential, and increased LDH in H9c2 cell supernatant. Na+-K+ATPase and Ca2+-Mg2+ATPase in cells and mitochondrial respiratory enzyme chain complex I, IV activity decreased; Angelica polysaccharide can inhibit cell apoptosis in a concentration-dependent manner, increase mitochondrial membrane potential, reduce LDH in supernatant, increase Na+-K+ATPase, Ca2+-Mg2+ATPase and mitochondrial respiratory enzyme chain complex I, IV activity. Conclusion: Angelica polysaccharide can protect cardiomyocytes from hypoxia-reoxygenation injury by improving myocardial cell energy metabolism disorder caused by hypoxia-reoxygenation.
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2019, Vol. 25 
