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| Detection Value of Th Cell Level in Peripheral Blood in Hepatitis Cirrhosis and Hepatocellular Carcinoma |
| CHEN Tian, et al |
| The Eleventh People's Hospital of Shenzhen, Guangdong Shenzhen 518109, China |
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Abstract Objective: To explore the detection value of peripheral blood Th cells in hepatitis, cirrhosis and liver cancer. Methods: 91 patients with liver diseases admitted by our department from March 2017 to March 2018 were selected as subjects, among which 34 were patients with chronic hepatitis b, 31 were patients with liver cirrhosis and 26 were patients with liver cancer. Meanwhile, 30 healthy subjects with normal liver function were selected as the control group. Using enzyme-linked immunosorbent (ELISA) method above and the control group in the peripheral blood of patients with Th cytokines - Th1 class factor [interferon (IFN) - gamma, interleukin (IL) - 12, tumor necrosis factor (TNF) - alpha], Th2 factor [IL - 4), IL - 6, IL - 10], the cells and the determination of Th cells (CD3 +, CD4 +, CD8 +). Results: IL-2, IFN-gamma, TNF-a, IL-4 and IL-10 in hepatitis B group were higher than those in cirrhosis group, while IL-6 was lower than that in cirrhosis group (P<0.05). The levels of IL-12, IL-6 and IL-10 in the hepatitis b group were higher than those in the liver cancer group, while the levels of ifn-fractionation and TNF- were significantly lower than those in the liver cancer group (P<0.05). The levels of ifn-beta, TNF-, IL-4 and IL-10 in the liver cirrhosis group were lower than those in the liver cancer group and IL-6 was higher than those in the liver cancer group, with statistically significant differences (P<0.05). IL-12, ifn-beta, TNF-, IL-4, IL-6, and IL-10 in the hepatitis b group and the cirrhosis group were lower than those in the control group, with statistically significant differences (P<0.05). IL-12, ifn-fractionation, IL-4, IL-6, and IL-10 in the liver cancer group were all lower than those in the control group, with statistically significant differences (P<0.05). There were also statistically significant differences in CD3+, CD4+, CD8+ and CD4+/CD8+ between the hepatitis B group, the liver cirrhosis group and the liver cancer group (P<0.05). Conclusion: The expression of Th1 and Th2 cytokines is abnormal in patients with hepatitis, cirrhosis and hepatocellular carcinoma. The balance of Th1/Th2 is destroyed and the clearance of HBV virus is inhibited. The detection of Th1/Th2 cytokines in patients with hepatitis, cirrhosis and hepatocellular carcinoma should be emphasized
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2019, Vol. 25 
