丙戊酸钠协同紫杉醇对肺癌A549细胞株血管新生相关因子表达的影响

doi:10.3969/j.issn.1006-6233.2017.03.004

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Abstract Objective: To investigate the effects of valproic acid ,one of histone deacetylase(HDAC) inhibitors, combinations with PTX, on the expression of vascular endothelial growth factor (VEGF) and Ang-2 of the lung cancer cell line A549 cell. Explore their significance of cell proliferation, apoptosis and metastasis on A549 cell. Methods: Set the A549 cell to four groups:control group,PTX group,VPA group and VPA combined with PTX group. Determine the mRNA and protein of VEGF and Ang-2 by quantitative PCR and Western Blot. Results: The PTX group,VPA group and VPA combined with PTX can downregulate the VEGF and Ang-2 in mRNA and protein level. The combination group of lowest level of these two genes and protein. The difference between the groups was statistically significant. Conclusion: HDAC inhibitor valproic acid may depend on its regulation of histone to inhibite cell proliferation , promote cell apoptosis of A549 cell and inhibit the angiogenesis by regulating the VEGF and Ang-2 expression.

Key words： Lung cancer Histone deacetylase inhibitor VPA VEGF Ang-2

Fund:河北省医学科学研究重点课题计划项目,(编号:20120163);河北省承德市科学技术研究与发展计划项目,(编号:201422027);承德医学院附属医院科研青年基金项目,(编号:201403)

Corresponding Authors: 李青山

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2017.03.004 OR http://www.hbyxzzs.cn/EN/Y2017/V23/I3/363

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