|
|
|
| Effect of Ribozyme on Blockade of IGF-1 and IGF-1R Expression as Well as Inhibition of Scar Formation |
| DENG Zhongyuan, LI Xiaojian, HOU Haijing, et al |
| Guangzhou Red Cross Hospital Affiliated to Jinan University, Guangdong Guangzhou 510220, China |
|
|
|
|
Abstract Objective: To discuss insulin-like growth factor receptor 1 (IGF-1) and its receptor (IGF-1R) expression in scar tissue and the effect of ribozyme on the expression level. Methods: 18 cases of Pathological scar tissue from operation excision (9 cases of scar keloid, 9 cases of scar hyperplasia ) were selected in Department of Burn and Plastics, Guangzhou Red Cross Hospital from 2015 May to 2016 July, meanwhile 20 cases of normal skin tissue specimens were collected. The expression level of tissue was observed by using hematoxylin eosin staining, the expression level of IGF-1 mRNA in each tissue specimen and its receptor mRNA were detected by using real-time fluorescence quantitative PCR, scar tissue fibroblasts were derived to culture cells to obtained IGF-1 and its receptor ribozyme, the cells in the logarithmic growth phase with good growth state in 2-4 generation + ribozyme were selected to culture,72h later, the expression level of IGF-1 mRNA and its receptor mRNA were detected by using real-time fluorescence quantitative PCR. Results: The differences were statistically significant in the expression level of IGF-1 mRNA in normal skin, hypertrophic scar and keloid tissue and its receptor mRNA (P <0.05), the comparison between groups showed that normal skin hypertrophic scar tissue < hypertrophic scar tissue < scar keloid tissue and the differences were statistically significant (P <0.05).Conclusion: The expression level of IGF-1 mRNA in scar tissue and its receptor mRNA are significantly higher than those in normal tissues, ribozyme has the effect on blockade of IGF-1 mRNA and its receptor mRNA expression, which may play a role in inhibiting scar formation.
|
|
|
|
|
|
[1] 席文群,成洪波,张国明,等.慢病毒介导的RNA干扰技术在滤过术后抗瘢痕的研究[J].眼科新进展,2013,33(5):489~492.
[2] 粱茶,朱斌,唐冰,等.胰岛素样生长因子1在病理性瘢痕中的表达[J].中国现代医生,2012,50(21):24~25.
[3] 刘爱东,王玥,庞久玲,等.基质金属蛋白酶7和转化生长因子β1在病理性瘢痕中的表达:与正常瘢痕及正常皮肤组织比较[J].中国组织工程研究,2012,16(7):1165~1168.
[4] 刘杜鹃,张可佳,丁玉红,等.病理性瘢痕综合治疗的研究进展[J].中国老年学杂志,2013,33(7):1731~1733.
[5] 胡成久,郭瑞珍,王燕.皮肤病理性瘢痕和瘢痕癌中Ras基因家族12/13位密码子突变的检测[J].实用医学杂志,2012,28(4):576~578.
[6] 付晋凤,谭加.病理性瘢痕的发生机制与修复[J].中华损伤与修复杂志(电子版),2013,8(4):6~12.
[7] Saber H, Himali J J, Beiser A, et al. Abstract T P130: High Serum Insulin-Like Growth Factor 1 is Associated with Lower Risk of Ischemic Stroke: The Framingham Heart Study[J].Stroke, 2015, 46(1):~130.
[8] 蔡浩, 唐冰,朱家源,等.病理性瘢痕中胰岛素样生长因子1及受体mRNA的表达[J].中国组织工程研究,2012,16(46):8629~8635. |
|
|
|
2017, Vol. 23 
