静态磁场通过激活FGFR1/JAK2/STAT3信号通路促进皮肤成纤维细胞的增殖活性和迁移表型

doi:10.3969/j.issn.1006-6233.2024.05.09

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Abstract Objective: To investigate the regulatory effects of static magnetic field (SMF) on the proliferation activity and migration phenotype of skin fibroblasts and its potential mechanism. Methods: Human skin fibroblasts (HSFs) were divided into five groups: control group, SMF group, SMF+AG group, SMF+LCK group, and SMF+Stattic group. The control group was HSFs cultured normally. HSFs cells in the SMF group were exposed to 1mT SMF. The other three groups were pre-incubated with inhibitors of FGFR1, JAK2, and STAT3 (3 μmoL/L AG, 3 nmoL/L LCK, and 20 μmoL/L Stattic) together with HSFs and exposed to 1 mT SMF. All groups were treated for 24 hours. Cell Counting Kit-8 (CCK-8) was used to analyze cell proliferation activity. ELISA was used to detect the level of Bcl-2-associated X protein (BAX). Western blot was used to detect the expression of apoptotic marker cleaved-caspase3 and FGFR1, JAK2, STAT3, phosphorylated (p)-FGFR1, p-JAK2, p-STAT3, and the expression of cell migration-related phenotypes high-mobility group box 1 (HMGB1), vimentin, vascular endothelial growth factor A (VEGFA), matrix metallopeptidase-9 (MMP-9), and MMP-2. Results: Compared with the control group, the cell proliferation activity in the SMF group was increased, the BAX level was decreased, the protein expression level of cleaved-caspase3 was down-regulated, and the expression levels of p-FGFR1, p-JAK2, p-STAT3, HMGB1, vimentin, VEGFA, MMP-9, and MMP-2 were all significantly up-regulated (all P<0.05). Compared with the SMF group, the cell proliferation activity in the SMF+AG group was decreased, the BAX level was increased, the protein expression level of cleaved-caspase3 was up-regulated, and the expression levels of p-FGFR1, p-JAK2, p-STAT3, HMGB1, vimentin, VEGFA, MMP-9, and MMP-2 were all significantly down-regulated (all P<0.05). Compared with the SMF group, the cell proliferation activity in the SMF+LCK group was decreased, the BAX level was increased, the protein expression level of cleaved-caspase3 was up-regulated, and the expression levels of JAK2, p-JAK2, p-STAT3, HMGB1, vimentin, VEGFA, MMP-9, and MMP-2 were all significantly down-regulated (all P<0.05). Compared with the SMF group, the cell proliferation activity in the SMF+Stattic group was decreased, the BAX level was increased, the protein expression level of cleaved-caspase3 was up-regulated, and the expression levels of STAT3, p-STAT3, HMGB1, vimentin, VEGFA, MMP-9, and MMP-2 were all significantly down-regulated (all P<0.05). Conclusion: SMF promotes the proliferation activity and migration phenotype of skin fibroblasts by activating the FGFR1/JAK2/STAT3 signaling pathway.

Key words： Static magnetic field Skin fibroblasts FGFR1/JAK2/STAT3 signaling pathway Proliferation Migration

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2024.05.09 OR http://www.hbyxzzs.cn/EN/Y2024/V30/I5/750

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