CircRTN4调节miR-224-5p/MYT1L轴对胶质母细胞瘤细胞恶性生物学行为的影响

doi:10.3969/j.issn.1006-6233.2024.01.03

Abstract
Figure/Table
References
Related Citation (0)

Download: PDF (1966 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To investigate the effect of circRNA RTN4 (CircRNA) on the malignant biological behavior of glioblastoma (GM) cells by regulating miR-224-5p/myelin transcription factor-1-like (MYT1L) axis. Methods: U251 cells in logarithmic growth phase were divided into the following groups: blank group, negative control (sh NC) group, silenced CircRTN4 shRNA (sh CircRTN4) group, sh CircRTN4 + inhibitor control (inhibitor NC) group, and sh CircRTN4 + miR-224-5p inhibitor (miR-224-5p inhibitor) group. Transwell experiment, flow cytometry, CCK-8, qRT-PCR, Western blot, and dual-luciferase assay were used to detect cell migration and invasion, apoptosis, proliferation, CircRTN4, miR-224-5p, MYT1L mRNA expression, proliferation protein (ki-67), apoptosis protein (cleaved Caspase-3), and MYT1L protein expression. The targeting relationships between miR-224-5p and CircRTN4, MYT1L were verified. qRT-PCR was used to detect the mRNA expression levels of CircRTN4, miR-224-5p, and MYT1L in GBM cell lines (U118, U87, U251, LN229) and normal human astrocytes (NHA) cells. Results: In U118, U87, U251, and LN229 cells, CircRTN4 and MYT1L mRNA expression increased significantly, while miR-224-5p expression decreased significantly compared to NHA cells (P<0.05). miR-224-5p had targeting relationships with CircRTN4 and MYT1L. Compared with the blank group and sh NC group, the sh CircRTN4 group showed significantly decreased migration, invasion, 24h, 48h A450 values, ki-67, CircRTN4, MYT1L mRNA, and protein expression, increased apoptosis rate, and cleaved Caspase-3 expression (P<0.05). Compared with the sh CircRTN4 + inhibitor NC group, the sh CircRTN4 + miR-224-5p inhibitor group showed significantly increased migration, invasion, 24h, 48h A450 values, ki-67, MYT1L mRNA, and protein expression, decreased apoptosis rate, and cleaved Caspase-3 expression (P<0.05). CircRTN4 expression showed no statistical difference (P>0.05). In vivo experiments demonstrated that interfering with CircRTN4 significantly inhibited tumor mass in nude mice (P<0.05). Conclusion: Silencing CircRTN4 regulates the miR-224-5p/MYT1L axis and inhibits the malignant biological behavior of GM cells.

Key words： Glioblastoma CircRTN4 MiR-224-5p/MYT1L axis Malignant biological behavior

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

URL:

http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2024.01.03 OR http://www.hbyxzzs.cn/EN/Y2024/V30/I1/15

[1] Yuan F,Zhang S,Sun Q,et al.Hsa_circ_0072309 enhances autophagy and TMZ sensitivity in glioblastoma[J].CNS Neurosci Ther,2022,28(6):897-912.
[2] Liu J,Zhao K,Huang N,et al.Circular RNAs and human glioma[J].Cancer Biol Med,2019,16(1):11-23.
[3] Wong C H,Lou U K,Fung F K,et al.CircRTN4 promotes pancreatic cancer progression through a novel CircRNA-miRNA-lncRNA pathway and stabilizing epithelial-mesenchymal transition protein[J].Mol Cancer,2022,21(1):10-24.
[4] Zheng S Q,Qi Y,Wu J,et al.CircPCMTD1 acts as the sponge of miR-224-5p to promote glioma progression[J].Front Oncol,2019,9(1):398-410.
[5] Yu Z,Liu Y,Li Y,et al.miRNA-338-3p inhibits glioma cell proliferation and progression by targeting MYT1L[J].Brain Res Bull,2022,179(1):1-12.
[6] Cao Q,Shi Y,Wang X,et al.Circular METRN RNA hsa_circ_0037251 promotes glioma progression by sponging miR-1229-3p and regulating mTOR expression[J].Sci Rep,2019,9(1):19791-19800.
[7] Gao Y,Gao J,Lin F,et al.CircRNAs in tumor radioresistance[J].Biomolecules,2022,12(11):1586-1593.
[8] Qi Y,Ma N,Chen X,et al.CircRtn4 acts as the sponge of miR-24-3p to promote neurite growth by regulating CHD5[J].Front Mol Neurosci,2021,14(1):660429-660440.
[9] Li J,Sun D,Pu W,et al.Circular RNAs in cancer:biogenesis,function and clinical significance[J].Trends Cancer,2020,6(4):319-336.
[10] Li J,Liu X,Li C,et al.miR-224-5p inhibits proliferation,migration,and invasion by targeting PIK3R3/AKT3 in uveal melanoma[J].Cell Biochem,2019,120(8):12412-12421.
[11] Zhang Q,Chen S,Zhen Y,et al.Circular RNA circFGFR1 functions as an oncogene in glioblastoma cells through sponging to hsa-miR-224-5p[J].Immunol Res,2022,2022(1):251-264.
[12] Zang C S,Huang H T,Qiu J,et al.MiR-224-5p targets EGR2 to promote the development of papillary thyroid carcinoma[J].Eur Rev Med Pharmacol Sci,2020,24(9):4890-4900.
[13] Wang B,Li D,Yao Y,et al.The crucial role of DNA-dependent protein kinase and myelin transcription factor 1-like protein in the miR-141 tumor suppressor network[J].Cell Cycle,2019,18(21):2876-2892.