大麻二酚抑制小鼠脊髓损伤后炎症反应及促进功能恢复的研究

doi:10.3969/j.issn.1006-6233.2017.05.018

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Abstract Objective: To investigate the inhibition of inflammation by cannabidiol in a mouse model of spinal cord injury and study its mechanism. Methords: 24 male C57Bl/6 mice were randomized into 3 groups: sham-operation group, control group and medication group. We established an spinal cord injury model by clip method, the mice in sham-operate group and control group were administered with a mixture of 1% of tween 80 and sterile saline (0.25mL/10g) and medication group were treated with cannabidiol (30mg/kg ; was dissolved in a mixture of 1% of Tween 80 and sterile saline (1.2mg/mL)) by intraperitioneal injection at 1h、12h、24h、36h、48h、60h after operation.72h after operation , BMS test was used to evaluate the neurological score, the expression levels of HMGB1 and TNF-αin spinal cord tissue were assayed by ELISA. Rsults: The BMS test showed that medication group had a higher score than the control group(P<0.05). The expression levels of HMGB1 and TNF-α in medication group were significant reduced as comparison with control group P<0.05), but significant increased than the sham-operation group(P<0.05). Conclusion: Cannabidiol can inhibit inflammation in a mouse model of spinal cord injury via inhibiting the expression of HMGB1.

Key words： Cannabidiol Spinal cord injury Inflammation Motor function

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2017.05.018 OR http://www.hbyxzzs.cn/EN/Y2017/V23/I5/767

[1] Festoff, B W.Designing drugs that encourage spinal cord injury healing[J].Expert Opinionon Drug Discovery,2014, 9(10):1151~1165.
[2] Frank MG, Weber M D, Watkins L R,et al. Stress sounds the alarmin: The role of the danger-associated molecular pattern HMGB1 in stress-induced neuroinflammatory priming[J].Brain Behavior and Immunity,2015, 48:1~7.
[3] 张莹,杨三强,刘均港,等.血浆置换对系统性红斑狼疮患者外周血HMGB-1、TNF-α水平的影响[J].山东医药,2013,53(18):82~83.
[4] Burstein S. Cannabidiol (CBD) and its analogs: a review of their effects on inflammation[J].Bioorganic & Medicinal Chemistry,2015, 23(7):1377~1385.
[5] Lusilla-Palacios P, Castellano-Tejedor C.Spinal cord injury and substance use: a systematic review[J].Adicciones,2015, 27(4):294~311.
[6] Wang J, Pearse D D.Therapeutic hypothermia in spinal cord injury: the status of its use and open questions[J].International Journal of Molecular Sciences,2015, 16(8):16848~16879.
[7] Sharma H S.Early microvascular reactions and blood-spinal cord barrier disruption are instrumental in pathophysiology of spinal cord injury and repair: novel therapeutic strategies including nanowired drug delivery to enhance neuroprotection[J].Journal of Neural Transmission,2011, 118(1):155~176.
[8] Schiavon A P, Soares L M, Bonato J M,et al.Protective effects of cannabidiol against hippocampal cell death and cognitive impairment induced by bilateral common carotid artery occlusion in mice[J].Neurotoxicity Research,2014, 26(4), 307~316.
[9] Frank M G, Weber M D, Fonken L K,et al.The redox state of the alarmin HMGB1 is a pivotal factor in neuroinflammatory and microglial priming: A role for the NLRP3 inflammasome[J].Brain Behavior and Immunity,2016,215~224.
[10] Hayakawa K, Mishima K, Nozako M,et al. Delayed treatment with minocycline ameliorates neurologic impairment through activated microglia expressing a high-mobility group box1-inhibiting mechanism[J].Stroke,2008, 39(3):951~958.
[11] Gelderblom M, Sobey C G, Kleinschnitz C,et al.Danger signals in stroke[J].Ageing Research Reviews,2015:77~82.
[12] Vénéreau E, Ceriotti C, Bianchi M E.DAMPs from Cell Death to New Life[J].FrontiersInImmunology,2015, 18(6): 422.
[13] 王晓燕,张流忠,周莉,等.脓毒症患者血清CRP、TNF-α水平变化及意义[J].山东医药,2012,58(34):34~35.
[14] Cong L, Chen W.Neuroprotective effect of ginsenoside rd on spinal cord injury rats[J].Basic & Clinical Pharmacology & Toxicology,2016, 119(2): 193~201.
[15] Song F, Zeng K, Liao L,et al.Schizandrin a inhibits microglia-mediated neuroninflammation through inhibiting TRAF6-NF-kB and Jak2-Stat3 signaling pathways[J].PLoS One,2016, 11(2).
[16] Han B, Lu Y, Zhao H,et al.Electroacupuncture modulated the inflammatory reaction in MCAO rats via inhibiting the TLR4/NF-κB signaling pathway in microglia[J].Journal of Clinical and Experimental Pathology,2015, 8(9):11199~11205.
[17] Maier I C, Schwab M E.Sprouting,regeneration and circuit formation in the injured spinal cord: factors and activity[J].Philosophical Transactions of the Royal Society B,2006,361(1473): 1611~1634.