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| Mechanistic of Gas6/MerTK Pathway Induces Mitochondrial Autophagy in the Progression of Lung Cancer |
| LU Qin, ZHANG Tingting, CHEN Mingzhi |
| Yixing People's Hospital / Yixing Hospital Affiliated to Jiangsu University, Jiangsu Yixing 214200, China |
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Abstract Objective: To investigate the possible mechanism of mitochondrial autophagy in the progression of lung cancer by downregulating the Gas6/MerTK signaling pathway. Methods: Calu-1 and A-427 cell lines were cultured and divided into (1) control group, Gas6 intervention (downregulation of Gas6) group, (2) control group, rapamycin (autophagy inducer, RAPA) group. Western blotting was used to detect the protein expression levels of Gas6, MerTK, LC3Ⅱ, and Beclin1. Transmission electron microscopy was employed to observe autophagic vesicles in cells. Colony formation assay was conducted to assess the cloning ability of Calu-1 and A-427 cells. CCK-8 assay was performed to measure cell viability. Scratch assay was used to evaluate the migration ability of Calu-1 and A-427 cells. Immunofluorescence was utilized to detect the expression levels of LC3 in Calu-1 and A-427 cells. Results: Compared with the control group, the Gas6 intervention group showed a significant decrease in Gas6 and MerTK protein levels in Calu-1 and A-427 cells (P<0.05), a significant increase in LC3Ⅱ and Beclin1 protein levels (P<0.05), and the formation of autophagic vesicles with abundant autophagic vacuole accumulation. The cloning ability, cell viability, and migration distance of Calu-1 and A-427 cells were significantly reduced (P<0.05). Compared with the control group, the RAPA group exhibited an increase in LC3 protein expression in Calu-1 and A-427 cells (P<0.05). The cloning ability, cell viability, and migration distance of Calu-1 and A-427 cells were significantly reduced (P<0.05). Conclusion: Downregulating the Gas6/MerTK signaling pathway can induce mitochondrial autophagy in Calu-1 and A-427 cells and inhibit their proliferation, activity, and migration abilities.
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2023, Vol. 29 
