西洋参总皂苷通过Nrf2-ARE通路对压力性尿失禁的治疗作用

doi:10.3969/j.issn.1006-6233.2023.11.09

Abstract
Figure/Table
References
Related Citation (3)

Download: PDF (2413 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To explore the therapeutic effect of total saponins of American ginseng on stress urinary incontinence by regulating Nrf2/ARE pathway. Methods: A stress urinary incontinence rat model was established.Sixty rats were randomly assigned to the following groups:Control group,Stress Urinary Incontinence (SUI) group,and SUI groups treated with low,medium,and high doses of American Ginseng Total Saponins (PQSL,PQSM,PQSH).Additionally,one SUI group received Nrf2-specific inhibitor ML385 (PQSH+ML385).Each group consisted of 10 rats.The positive sneezing rates were recorded.Urodynamic parameters,including MBC,LPP,ALPP,as well as intravesical pressure (voided volume,residual volume,voiding efficiency) were measured.Masson staining was used to assess collagen fiber levels in urethral tissues.Oxidative stress indicators (MDA,SOD,GSH-Px) were determined in urethral tissues.Western blot analysis was performed to assess the expression of Nrf2,HO-1,and NQO1 in brain tissue. Results: In the Control group,sneezing tests were consistently negative.In the SUI group,all 50 rats showed positive sneezing test results.The positive sneezing rates in PQSL,PQSM,and PQSH groups were 60% (6/10),40% (4/10),and 20% (2/10),respectively,whereas in the PQSH+ML385 group,the rate was 70% (7/10),and in the SUI group,it was 80% (8/10).Compared to the Control group,the SUI group exhibited reduced MBC,LPP,ALPP levels,urinary efficiency,GSH-Px content,and SOD activity,along with increased urine volume,residual volume,and MDA content (P<0.05).In comparison with the SUI group,the PQSL,PQSM,and PQSH groups demonstrated elevated MBC,LPP,ALPP levels,urinary efficiency,GSH-Px content,and SOD activity,and reduced urine volume,residual volume,and MDA content,with effects showing concentration-dependent patterns (P<0.05).When compared to the PQSH group,the PQSH+ML385 group displayed decreased MBC,LPP,ALPP levels,urinary efficiency,GSH-Px content,and SOD activity,alongside increased urine volume,residual volume,and MDA content (P<0.05).Compared to the Control group,the SUI group showed significantly decreased expression of Nrf2,HO-1,and NQO1 in urethral tissue (P<0.05).In contrast,the PQSL,PQSM,and PQSH groups exhibited significantly increased expression of Nrf2,HO-1,and NQO1 in urethral tissue,with effects being dose-dependent (P<0.05).When compared to the PQSH group,the PQSH+ML385 group showed significantly decreased expression of Nrf2,HO-1,and NQO1 in urethral tissue (P<0.05). Conclusion: American Ginseng Total Saponins may improve urinary function in stress urinary incontinence by reducing oxidative stress in the urethra.This effect appears to be associated with the activation of the Nrf2-ARE signaling pathway.

Key words： American ginseng total saponin Stress urinary incontinence Voiding function Nrf2/ARE signaling

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

URL:

http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.11.09 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I11/1805

[1] Abdel-Fattah M,Cooper D,Davidson T,et al.Single-incision mini-slings for stress urinary incontinence in women[J].New England Journal of Medicine,2022,386(13):1230-1243.
[2] Bergstrom B S.Stress urinary incontinence is caused predominantly by urethral support failure[J].International Urogynecology Journal,2022,33(3):523-530.
[3] Sawettikamporn W,Sarit-Apirak S,Manonai J.Attitudes and barriers to pelvic floor muscle exercises of women with stress urinary incontinence[J].BMC Women's Health,2022,22(1):1-8.
[4] Chen WH,Jiang YH,Kuo HC.Urinary oxidative stress biomarkers in the diagnosis of detrusor overactivity in female patients with stress urinary incontinence[J].Biomedicines,2023,11(2):357.
[5] Liu X,Guan PY,Yu CT,et al.Curcumin alleviated lipopolysaccharide-induced lung injury via regulating the Nrf2-ARE and NF-κ B signaling pathways in ducks[J].Journal of the Science of Food and Agriculture,2022,102(14):6603-6611.
[6] Sun Q,Zeng C,Du L,et al.Mechanism of circadian regulation of the NRF2/ARE pathway in renal ischemiareperfusion[J].Experimental and Therapeutic Medicine,2021,21(3):1-1.
[7] 汤慧丽,王宪昌,李佳,等.西洋参皂苷类成分及其生物活性、质量控制的研究进展[J].中国中药杂志,2022,47(1):36-47.
[8] 刘静,陈希,金宏飞,等.西洋参茎叶总皂苷抑制氧糖剥夺/复氧引起的BV-2小胶质细胞炎性活化作用及其机制研究[J].上海中医药大学学报,2023,37(2):7-13.
[9] 陈韶慧,燕美琴,潘晋兵,等.人参皂苷Rb1对雌性大鼠产后压力性尿失禁模型修复作用的研究[J].中国药物与临床,2021,21(16):2770-2772.
[10] Amend B,Harland N,Knoll J,et al.Large animal models for investigating cell therapies of stress urinary incontinence[J].International Journal of Molecular Sciences,2021,22(11):6092.
[11] Liu J,Liu Z,Tang Y,et al.Treatment with platelet-rich plasma attenuates proprioceptor abnormalities in a rat model of postpartum stress urinary incontinence[J].International Urogynecology Journal,2022,33(8):2159-2167.
[12] Lu D,Jiayi L I.Research progress of fractional CO₂ laser therapy in female stress urinary incontinence[J].Journal of Shanghai Jiao Tong University (Medical Science),2022,42(5):685.
[13] Luo L,Li L,Chen X,et al.Research progress on compliance of pelvic floor muscle training in patients with urinary incontinence[J].Open Journal of Urology,2021,11(6):187-199.
[14] Monti M,Fischetti M,Santangelo G,et al.Update on surgical treatment of female stress urinary incontinence[J].Minerva Obstetrics and Gynecology,2020,73(2):140-144.
[15] Chapple C R.Contemporary surgical management of stress urinary incontinence[J].Current Opinion in Urology,2020,30(2):271.