miR-891a-5p对结肠癌细胞肿瘤生物学行为和血管生成潜能的作用

doi:10.3969/j.issn.1006-6233.2023.09.01

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Abstract Objective: To explore the effects of miR-891a-5p on the proliferation, invasion, migration, apoptosis and angiogenesis of colon cancer cells and its mechanism. Methods: The cell with the highest expression of miR-891a-5p in each cell line of FHC, SW480, HCT116, HCT8, and SW620 was screened by PCR for SW480, and miR-891a-5p mimic (miR-891a-5p mimic), miR-891a-5p inhibitor (miR-891a-5p inhibitor) and their corresponding controls were transfected into SW480 cells. SW480 were divided into OE-NC group and OE-NLRP7, and the proliferative ability of SW480 was determined by CCK-8 method, the invasive ability of SW480 was determined by Transwell test, and the migratory ability of SW480 was determined by scratch test; the protein expression of VEGF and MMP-9 was analyzed by Western blot; and the angiogenic ability was examined by HUVEC angiogenesis assay. The targeting relationship between miR-891a-5p and NLRP7 was validated with luciferase reporter gene assay; the interaction between miR-891a-5p and NLRP7 was analyzed with RNA immunoprecipitation. Results: The expression of miR-891a-5p in colon cancer cells was significantly higher than that in human normal colon epithelial cells, and the expression of NLRP7 in colon cancer cells was significantly lower than that in normal stem cells. Inhibition of miR-891a-5p significantly decreased the proliferation, invasion, migration and angiogenesis of SW480 cells (P<0.01). The protein expression levels of VEGF and MMP-9 were significantly decreased (P<0.01). The results of targeting relationship showed that there was a targeting relationship between miR-891a-5p and NLRP7. NLRP7 overexpression could reduce the proliferation, migration, and angiogenesis of SW480 cells (P<0.01). Conclusion: miR-891a-5p can regulate NLRP7 to promote the proliferation, invasion, migration and angiogenesis of colon cancer cells to accelerate the development of colon cancer.

Key words： miR-891a-5p Colon cancer NLRP7 Angiogenesis

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	KANG Lili
	CHEN Zhaowei
	SONG Jian

Cite this article:

KANG Lili,CHEN Zhaowei,SONG Jian. Role of miR-891a-5p on Tumor Biological Behavior and Angiogenic Potential of Colon Cancer Cells[J]. HeBei Med, 2023, 29(9): 1409-1414.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.09.01 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I9/1409

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