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| Expression of Hepatocyte Growth Factor-1 in Human Glioma and Effect on Apoptosis of Glioma Cells |
| QUAN Junjie, QU Jianqiang, ZHOU Le, et al |
| The Second Affiliated Hospital of Xi'an Jiaotong University, Shanxi Xi'an 710004, China |
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Abstract Objective: To investigate the expression of hepatocyte growth factor -1 (PTP4A1) in glioma and effect on the apoptosis of glioma cells. Methods: Tissue protein in brain glioma and tumor adjacent tissues were extracted by total protein extraction kit; PTP4A1 protein expression were detected by western blot; siRNA-NC and PTP4A1-siRNA was transfected into the human glioma U251 cells of the logarithmic growth phase, and without any siRNA transfection as the control group, each group of cells transfected for 48h, expression of PTP4A1, Ki67, PCNA, Cleaved, Caspase3, β-catenin, CyclinD1, C-myc protein were detected by Western blot; cell proliferation was detected by CCK8 assay; cell apoptosis was detected by flow cytometry. Results: The expression of PTP4A1 protein in gliomas was significantly higher than that in the tumor adjacent tissues (P<0.01); the expression of PTP4A1 protein in siRNA-NC group and control group had no significant difference (P>0.05), expression of PTP4A1 protein in PTP4A1-siRNA group were significantly lower than the control group (P<0.01); compared with the control group and siRNA-NC group, the cell survival rate and the expression of Ki67, PCNA, β-catenin, CyclinD1, C-myc protein in PTP4A1-siRNA group were significantly decreased, cell apoptosis rate and expression of Cleaved Caspase3 protein was significantly increased (P<0.01). Conclusion: PTP4A1 is higher expression in glioma tissues. And silented PTP4A1 expression can inhibit the proliferation and induce apoptosis of human glioma U251 cells by down regulating the Wnt/β -catenin signaling pathway.
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2017, Vol. 23 
