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| The Mechanism of SFRP1 on the Activity and Angiogenesis of Dental Pulp Stem Cells through MAPK Signaling Pathway |
| CHE Yilei, FANG Lin |
| Aerospace Center Hospital, Beijing 100049, China |
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Abstract Objective: To explore the molecular regulation mechanism of dental pulp stem cells. Methods: Human dental pulp stem cells (DPSC) were purchased and transfected with si-NC/SFRP1. The mRNA and protein expression level of SFRP1 was detected by qRT-PCR and Western blot. CCK-8 and flow cytometry were used to detect the proliferation activity and apoptosis of cells in each group. Cell wound healing and angiogenesis assays were used to detect the changes of cell migration and angiogenesis ability in each group. Western blot was used to detect the protein changes of p-p38 and p38 in MAPK signaling pathway. Results: After knocking down the expression of SFRP1 in DPSC, the proliferation activity of DPSC decreased and the apoptosis rate increased significantly. Cell migration and angiogenesis ability decreased significantly; the protein content of p-p38 in MAPK signaling pathway was significantly decreased. Conclusion: Knockdown of SFRP1 inhibited the proliferation and angiogenesis of dental pulp stem cells and promoted their apoptosis by inactivating the MAPK signaling pathway.
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[1] Li M,Zhao Y,Hao H,et al.Mesenchymal stem cell-based therapy for nonhealing wounds:Today and tomorrow[J].Wound Repair Regen,2015,23(4):465-482.
[2] Mazini L,Rochette L,Admou B,et al.Hopes and limits of adipose-derived stem cells (ADSCs) and mesenchymal stem cells (MSCs) in wound healing[J].Int Mol Sci,2020,21(4).
[3] Lv F J,Tuan R S,Cheung K M,et al.Concise review:The surface markers and identity of human mesenchymal stem cells[J].Stem Cells,2014,32(6):1408-1419.
[4] Zhang Y,Liu J,Zou T,et al.DPSCs treated by TGF-β1 regulate angiogenic sprouting of three-dimensionally co-cultured HUVECs and DPSCs through VEGF-Ang-Tie2 signaling[J].Stem Cell Res Ther,2021,12(1):281.
[5] Delle M S,Martellucci S,Clementi L,et al.In vitro conditioning determines the capacity of dental pulp stem cells to function as Pericyte-Like cells[J].Stem Cells Dev,2019,28(10):695-706.
[6] Adriztina I,Munir D,Sandra F,et al.Differentiation capacity of dental pulp stem cell into inner ear hair cell using an in vitro assay:a preliminary step toward treating sensorineural hearing loss[J].Eur Arch Otorhinolaryngol,2022,279(4):1805-1812.
[7] Nakashima M,Iohara K.Mobilized dental pulp stem cells for pulp regeneration:Initiation of clinical trial[J].Endod,2014,40(4 Suppl):26-32.
[8] Liu D,Yao S,Wise G E.Regulation of SFRP-1 expression in the rat dental follicle[J].Connect Tissue Res,2012,53(5):366-372. |
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2023, Vol. 29 
