circ_0001658通过靶向miR-1179调控乳腺癌细胞的增殖细胞周期和凋亡

doi:10.3969/j.issn.1006-6233.2023.08.04

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Abstract Objective: To investigate the effects of circ_0001658 on proliferation, cell cycle and apoptosis of breast cancer BT-549 cells and its molecular mechanism. Methods: A total of 30 breast cancer tissues and corresponding paracancer tissues were selected. BT-549 cells were cultured in vitro and divided into si-NC group, si-circ_0001658 group, miR-NC group, miR-1179 group, si-circ_0001658+anti-miR-NC group, si-circ_0001658+anti-miR-1179 group. RT-qPCR was used to detect the expression levels of circ_0001658 and miR-1179. The expression levels of circ_0001658 and miR-1179 were detected by RT-qPCR. Cell proliferation was detected by MTT. Cell cycle was detected by PI single staining. Cell apoptosis was detected by flow cytometry. The expression of related proteins was detected by Western blot. The targeting relationship between circ_0001658 and miR-1179 was detected by dual luciferase reporter assay. Results: circ_0001658 expression was significantly increased (1.00±0.11 vs 4.24±0.53) and miR-1179 expression was significantly decreased (1.00±0.10 vs 0.45±0.05) in breast cancer tissues in comparison with paracancerous tissues (P<0.05). Silencing circ_0001658 decreased the OD value of BT-549 cells (1.45±0.18 vs 0.81±0.07), the proportion of S phase cells (28.19±2.36 vs 15.33±1.16), the protein expression of PCNA (0.66±0.07 vs 0.34±0.04), CyclinD1 (0.69±0.08 vs 0.28±0.03) and Bcl-2 (0.59±0.06 vs 0.21±0.02), and increased the proportion of G₀-G₁ phase cells (47.35±3.28 vs 62.24±5.14), the apoptosis rate (6.58±0.75 vs 25.34±2.86), and the protein expression of p27 (0.36±0.03 vs 0.79±0.08) and Bax (0.25±0.03 vs 0.65±0.06) (P<0.05). Circ_0001658 regulation of miR-1179 expression (P<0.05), and down-regulated miR-1179 reversed the effects of circ_0001658 silencing (P<0.05). Conclusion: Silencing circ_0001658 inhibited BT-549 cell proliferation and promoted cell apoptosis through targeted up-regulation of miR-1179.

Key words： circ_0001658 miR-1179 Breast cancer Proliferation Cell cycle Apoptosis

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	LI Huan
	XIE Xianxin
	SUN Tao

Cite this article:

LI Huan,XIE Xianxin,SUN Tao. circ_0001658 Regulates Proliferation Cell Cycle and Apoptosis of Breast Cancer Cells by Targeting miR-1179[J]. HeBei Med, 2023, 29(8): 1252-1258.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.08.04 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I8/1252

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