血清HMGB1 Cav-1水平与股骨骨折髓内钉固定术后延迟愈合的关系

doi:10.3969/j.issn.1006-6233.2023.06.029

Abstract
Figure/Table
References
Related Citation (7)

Download: PDF (1431 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To investigate the relationship between serum high mobility group protein B1 (HMGB1) and caveolin-1 (Cav-1) levels and delayed healing after intramedullary nail fixation of femoral fractures. Methods: A total of 70 patients admitted to our hospital from January 2018 to July 2022 with delayed healing after intramedullary nail fixation of femur fractures were selected as the delayed healing group, and 70 patients with normal healing after intramedullary nail fixation of femur fractures were selected in a 1:1 ratio as the normal healing group. Clinical data of the patients were collected and serum HMGB1 and Cav-1 levels were measured by ELISA. The factors influencing delayed healing after intramedullary nail fixation of femoral fractures were analyzed by multi-factor logistic regression, and the ROC curve was used to analyze the predictive value of serum HMGB1 and Cav-1 levels on delayed healing after intramedullary nail fixation of femoral fractures. Results: Serum HMGB1 (2.71±0.42 ng/ml vs 3.68±1.04 ng/ml) and Cav-1 (10.85±1.89 ng/ml vs 13.03 ± 2.07 ng/ml) levels were reduced in the delayed healing group compared to the normal healing group (t=7.204, 6.497, all P<0.001). Multi-factor logistic regression analysis showed that increasing age (OR=1.095, 95% CI: 1.036 to 1.157) was an independent risk factor for delayed healing after intramedullary nail fixation of femoral fractures, and HMGB1 (OR=0.936, 95% CI: 0.904 to 0.969) and Cav-1 (OR=0.599, 95% CI: 0.470 to 0.763) were elevated as independent protective factors (all P<0.05). ROC curve analysis showed that serum HMGB1, Cav-1 levels combined predicted a greater area under the curve (AUC) for delayed healing after intramedullary nail fixation of femoral fractures than HMGB1, Cav-1 predicted individually (Z=2.352, 2.621, all P<0.05). Conclusion: Decreased serum HMGB1 and Cav-1 levels are closely associated with delayed healing after intramedullary nail fixation of femoral fractures and may be an auxiliary predictor of delayed healing after surgery.

Key words： Intramedullary nail fixation of femoral fractures Delayed healing High mobility group protein B1 Caveolin-1

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

URL:

http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.06.029 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I6/1032

[1] 中华医学会骨科学分会创伤骨科学组,中国医师协会骨科医师分会创伤专家工作委员会.成人股骨颈骨折诊治指南[J].中华创伤骨科杂志,2018,20(11):921-928.
[2] 陈文瑶,黄晶,阙祥勇,等.髓内钉固定治疗多节段股骨骨折的疗效分析[J].创伤外科杂志,2022,24(3):198-202.
[3] Yang J,Zhang X,Liang W,et al.Efficacy of adjuvant treatment for fracture nonunion/delayed union:a network meta-analysis of randomized controlled trials[J].BMC Musculoskelet Disord,2022,23(1):481.
[4] 王小璐,崔宇,张令强.促进骨折愈合的治疗策略及机制研究进展[J].生命科学,2021,33(1):121-130.
[5] 张子霄,卞涛.HMGB1参与慢性气道炎症性疾病的研究进展[J].国际呼吸杂志,2021,41(24):1890-1895.
[6] Yang J,Shah R,Robling AG,et al.HMGB1 is a bone-active cytokine[J].Cell Physiol,2008,214(3):730-739.
[7] 孙月彤,张炳强,邹伟.陷窝蛋白-1与干细胞衰老、自我更新的研究进展[J].生物技术通讯,2020,31(1):88-92.
[8] Lee YD,Yoon SH,Park CK,et al.Caveolin-1 regulates osteoclastogenesis and bone metabolism in a sex-dependent manner[J].Biol Chem,2015,290(10):6522-6530.
[9] Silverman SL,Kupperman ES,Bukata SV.Fracture healing:a consensus report from the international osteoporosis foundation fracture working group[J].Osteoporos Int,2016,27(7):2197-2206.
[10] Oyama M,Ukai T,Yamashita Y,et al.High-mobility group box 1 released by traumatic occlusion accelerates bone resorption in the root furcation area in mice[J].Periodontal Res,2021,56(1):186-194.
[11] Ranzato E,Patrone M,Pedrazzi M,et al.Hmgb1 promotes wound healing of 3T3 mouse fibroblasts via RAGE-dependent ERK1/2 activation[J].Cell Biochem Biophys,2010,57(1):9-17.
[12] Lee YD,Yoon SH,Park CK,et al.Caveolin-1 regulates osteoclastogenesis and bone metabolism in a sex-dependent manner[J].Biol Chem,2015,290(10):6522-6530.
[13] Zou B,Zhang Y,Li T,et al.A mutation of cysteine 46 in IKK-β promotes mPGES-1 and caveolin-1 expression to exacerbate osteoclast differentiation and osteolysis[J].Biochem Pharmacol,2020,2(172):113762.
[14] Liang LL,Su ZB.In vitro effect of caveolin-1 as a slow-release material on bone-tendon junction healing:a comparative study[J].Kaohsiung Med Sci,2019,35(3):175-182.
[15] 李蕴,刘邦忠,刘光华,等.低强度脉冲超声对兔桡骨骨折愈合及小窝蛋白-1基因表达的影响[J].复旦学报(医学版),2018,45(2):206-213.