载5-氟尿嘧啶细胞囊泡对结直肠癌细胞株HCT116的杀伤作用

doi:10.3969/j.issn.1006-6233.2023.06.03

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Abstract Objective: To explore the killing effect of 5-fluorouracil cell vesicles on colorectal cancer cell line HCT116. Methods: The HCT116 group, cell vesicles group (EVs suspension 5mL, 10⁶ cells/mL), 5-fluorouracil group (5-fluorouracil 5mL, 30μg/mL), and 5-fluorouracil drug-loaded vesicles group (EVs suspension 5mL, 10⁶ cells/mL+5-fluorouracil 5mL, 30μg/mL) were set up, and 6 parallel samples of each well of the above groups were incubated for 72h. After incubation, the levels of cell proliferation, invasion and apoptosis were determined, and the levels of miR-128 and PIK3 were determined by RT-PCR and western blotting. Results: The OD value, survival rate, number of monoclonal formation, number of membrane penetration, migration distance, apoptosis rate, miR-128 level, PI3K mRNA and protein levels in cell vesicles group were not significantly different from those in HCT116 group(P>0.05). OD value, survival rate, number of monoclonal formation, number of membrane penetration, migration distance, PI3KmRNA and protein levels in 5-fluorouracil group and 5-fluorouracil drug-loaded vesicles group were significantly lower than those in HCT116 group and cell vesicles group(P<0.05), and the apoptosis rate and the level of miR-128 were significantly higher than those in HCT116 group and cell vesicle group (P<0.05). OD value, survival rate, number of monoclonal formation, number of membrane penetration, migration distance, PI3K mRNA and protein levels in 5-fluorouracil drug-loaded vesicles group were significantly lower than those in 5-fluorouracil group, and the apoptosis rate and miR-128 level were significantly higher than those in 5-fluorouracil group(P<0.05). Conclusion: Extracellular vesicles loaded with 5-fluorouracil can significantly inhibit the proliferation and invasion level of colorectal cancer cell line HCT116, and enhance its apoptosis level, which may be related to the mechanism that extracellular vesicles loaded with 5-fluorouracil can promote the high expression of miR-128 and low expression of PI3K in colorectal cancer cell line HCT116.

Key words： 5-fluorouracil Colorectal cancer Proliferation of cells

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.06.03 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I6/893

[1] Xi Y,Xu P.Global colorectal cancer burden in 2020 and projections to 2040[J].Transl Oncol,2021,14(10):101174.
[2] Biller LH,Schrag D.Diagnosis and treatment of metastatic colorectal cancer:a review[J].JAMA,2021,325(7):669-685.
[3] Herrmann IK,Wood MJA,Fuhrmann G.Extracellular vesicles as a next-generation drug delivery platform[J].Nat Nanotechnol,2021,16(7):748-759.
[4] Verweij F J,Balaj L,Boulanger CM,et al.The power of imaging to understand extracellular vesicle biology in vivo[J].Nat Methods,2021,18(9):1013-1026.
[5] Guo Y,Guo Y,Chen C,et al.Circ3823 contributes to growth,metastasis and angiogenesis of colorectal cancer:involvement of miR-30c-5p/TCF7 axis[J].Mol Cancer,2021,20(1):93.
[6] He F,Song Z,Chen H,et al.Long noncoding RNA PVT1-214 promotes proliferation and invasion of colorectal cancer by stabilizing Lin28 and interacting with miR-128[J].Oncogene,2019,38(2):164-179.
[7] Wang H,Wang X,Zhang H,et al.The HSF1/miR-135b-5p axis induces protective autophagy to promote oxaliplatin resistance through the MUL1/ULK1 pathway in colorectal cancer[J].Oncogene,2021,40(28):4695-4708.
[8] Zhang W,Wang Z,Cai G,et al.Circ_DOCK1 regulates USP11 through miR-132-3p to control colorectal cancer progression[J].World Surg Oncol,2021,19(1):67.
[9] Zhang X,Li T,Han Y N,et al.miR-125b promotes colorectal cancer migration and invasion by dual-targeting CFTR and CGN[J].Cancers,2021,13(22):5710.
[10] Zheng J,Yang T,Gao S,et al.miR-148a-3p silences the CANX/MHC-I pathway and impairs CD8⁺ T cell-mediated immune attack in colorectal cancer[J].FASEB,2021,35(8):e21776.
[11] Zhou L,Li J,Tang Y,et al.Exosomal LncRNA LINC00659 transferred from cancer-associated fibroblasts promotes colorectal cancer cell progression via miR-342-3p/ANXA2 axis[J].Transl Med,2021,19(1):8.
[12] Bai L,Gao Z,Jiang A,et al.Circular noncoding RNA circ_0007334 sequestrates miR-577 to derepress KLF12 and accelerate colorectal cancer progression[J].Anticancer Drugs,2022,33(1):409-422.