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| Database-Based Exploration of Abnormal Expression and Prognosis of Centromeric Protein F in Lung Adenocarcinoma |
| ZHOU Meifeng, WANG Lin, WANG Haixia, et al |
| Department of Oncology, Hainan General Hospital/Hainan Affiliated Hospital of Hainan Medical University, Hainan Haikou 570311, China |
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Abstract Objective: To explore the expression of Centromeric protein F(CENPF)and its biological function in LUAD. Methods: CENPF mRNA expression in LUAD was analyzed by searching the Oncomine database, CENPF protein expression levels were determined by immunohistochemical (IHC) staining using the HPA database, and IHC images were obtained from the HPA database. In addition, subgroups and prognosis of LUAD were analyzed by using the Ualcan and GEPIA database tools, respectively. To further explore the CENPF sequence mutations in LUAD patients, the c-Bioportal website was applied. Finally, the LinkedOmics database was applied to analyze the co-expressed genes of CENPF in the LUAD cohort for functional and pathway analysis by the LinkInterpreter module. Results: CENPF was highly expressed in LUAD (P<0.05), which could lead to poor prognosis by Kaplan-Meier analysis (P<0.05). And CENPF mRNA expression was related to smoking status, TP53 mutation and tumor stage in LUAD patients (P<0.05). In addition, CENPF was mutated in LUAD, but it had no effect on LUAD prognosis (P=0.698). Co-expression analysis showed that CENPF co-expressed genes were closely related to cell cycle and cell division process. Conclusion: CENPF can be involved in the development of LUAD as a pro-oncogene and has potential applications as a diagnostic, prognostic indicator and targeted therapy for LUAD.
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2023, Vol. 29 
