miR-24-3p靶向BMP8B对成骨细胞功能影响的实验研究

doi:10.3969/j.issn.1006-6233.2023.01.0011

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Abstract Objective: To investigate the targeting relationship between miR-24-3p and bone morphogenetic protein 8B (BMP8B), and to analyze their effects on osteoblast function. Methods: Human periodontal ligament stem cells (hPDLSCs) were isolated and cultured in vitro and induced to differentiate into osteoblasts. The osteoblasts were identified by alkaline phosphatase (ALP) staining and alizarin red staining; the osteoblasts were divided into blank control group, negative control group (inhibitor-NC group), silencing miR-24-3p expression group (miR-24-3p-inhibitor group), and co-transfection group (miR-24-3p-inhibitor +BMP8B-siRNA group). Real-time fluorescent quantitative PCR method was used to detect the levels of miR-24-3p and BMP8B mRNA; CCK-8 method and flow cytometer were used to detect the proliferation and apoptosis of bone cells in each component; ALP activity detection kit was used to detect ALP activity; Western blotting was used to detect the levels of BMP8B protein and functional activity-related proteins (PICP, BGP, OPN) in each component of bone cells. The TargetScan database and the dual luciferase reporter gene experiment were used to predict and verify the targeting relationship between miR-24-3p and BMP8B respectively. Results: Compared with the blank control group and inhibitor-NC group, the osteoblast proliferation inhibition rate and apoptosis rate in the miR-24-3p-inhibitor group were decreased, ALP activity and expression of PICP, BGP, OPN were increased (P<0.05); compared with miR-24-3p-inhibitor group, the osteoblast proliferation inhibition rate and apoptosis rate in the miR-24-3p-inhibitor+BMP8B-siRNA group were increased, ALP activity and expression of PICP, BGP, OPN were decreased (P<0.05). The dual luciferase reporter gene experiment confirmed that miR-24-3p targeted regulation of BMP8B. Conclusion: Inhibition of miR-24-3p may promote the proliferation and differentiation of osteoblasts and inhibit cell apoptosis by targeting up-regulation of BMP8B expression.

Key words： miR-24-3p Bone morphogenetic protein 8B Osteoblasts Proliferation Apoptosis

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.01.0011 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I1/59

[1] Hirschfeld J,Reichardt E,Sharma P,et al.Interest in orthodontic tooth alignment in adult patients affected by periodontitis:a questionnaire-based cross-sectional pilot study[J].Periodontol,2019,90(9):957-965.
[2] Juan RGS,José ARC,Sandra OHG,et al.Efficacy of clindamycin compared with amoxicillin-metronidazole after a 7-day regimen in the treatment of periodontitis in patients with diabetes:a randomized clinical trial[J].Rmd Open,2020,8(1):1-9.
[3] 李军最,马鸳霞,李兴艳.长链非编码RNA调控脂肪干细胞成骨分化的研究进展[J].中华实验外科杂志,2019,36(1):186-189.
[4] Li Z,Sun Y,Cao S,et al.Downregulation of miR-24-3p promotes osteogenic differentiation of human periodontal ligament stem cells by targeting SMAD family member 5[J].Cell Physiol,2018,234(5):1-11.
[5] Mahli A,Seitz T,Beckrge T,et al.Bone morphogenetic protein-8B expression is induced in steatotic hepatocytes and promotes hepatic steatosis and inflammation in vitro[J].Cells,2019,8(5):457-463.
[6] 邓亚军,解琪琪,李文洲,等.miRNAs在破骨细胞中的研究进展[J].生命科学研究,2019,23(1):58-62.
[7] 张硕,王非凡,刘洪臣.环状RNA调控骨髓间充质干细胞成骨分化的研究进展[J].口腔颌面修复学杂志,2019,20(5):304-308.
[8] Wu Y,Lian K,Sun C.LncRNA LEF1-AS1 promotes osteogenic differentiation of dental pulp stem cells via sponging miR-24-3p[J].Mol Cell Biochem,2020,8(11):1-13.
[9] Nathir I,Hashim RD,Fawzi OF.Efficacy of hemodialysis and other therapeutic measures in controlling hypocalcemia,hyperphosphatemia and elevated ALP in a group of patients with end-stage renal disease[J].Ann Trop Med Public Health,2020,23(14):698-705.
[10] Yang C,Qiao S,Song Y,et al.Procollagen type I carboxy-terminal propeptide (PICP) and MMP-2 are potential biomarkers of myocardial fibrosis in patients with hypertrophic cardiomyopathy[J].Cardiovasc Pathol,2019,43(77):107150-107157.
[11] Zhang C,Ma K,Li WY.IL-6 Promotes Cancer stemness and oncogenicity in U2OS and MG-63 osteosarcoma cells by upregulating the OPN-STAT3 pathway[J].Cancer,2019,10(26):6511-6525.
[12] Yaghoobi A,Samani KG,Farrokhi E.The effect of hydro-alcoholic extract of nigella sativa on Bmp7 and Bmp8b expression in rats fed with a high-fat diet[J].Jundishapur Nat Pharm Prod,2020,7(2):56-60.
[13] Ghasemzadeh-Hasankolaei M,Sedighi-Gilani MA,Eslaminejad MB.Induction of ram bone marrow mesenchymal stem cells into germ cell lineage using transforming growth factor-β superfamily growth factors[J].Reprod Domest Anim,2018,49(4):588-598.
[14] Chen Z,Lu S,Xu M,et al.Role of miR-24,furin,and transforming growth factor-β1 signal pathway in fibrosis after cardiac infarction[J].Med Sci Monit,2017,5(23):65-70.