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| MicroRNA-122-5P Inhibits TPA-Induced Invasive Migration of SW480 Cells by Targeting MMP-9 to |
| ZHANG Lijun, SHI Hao, JIANG Zhuoyan, et al |
| The Affiliated Xinhua Hospital of Dalian University, Liaoning Dalian 116021, China |
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Abstract Objective: To study the effect of microRNA-122-5p on the expression, invasion and migration of MMP-9 in human colorectal cancer SW480 cells induced by TPA. Methods: Bioinformatics was used to screen colorectal cancer differential genes and predict their target miRNA. SW480 cells were treated with TPA (100 nM), and gene and protein expression levels were detected by RT-qPCR and Western blot; gelatin zymography was used to detect MMP-9 cell exocytosis; Transwell was used to detect cell invasion and migration ability. Results: MMP-9 was highly expressed in colon cancer tissues, and the upstream target gene miR-122-5P was screened; TPA induced a time-dependent upregulation of MMP-9 mRNA expression (P<0.01) and a downregulation of miR-122-5p expression (P<0.01); overexpression of miR-122-5p significantly downregulated TPA-induced MMP-9 mRNA and protein expression (P<0.05), the extracellular secretion of MMP-9 was reduced, and the invasion and migration ability of cells were significantly decreased (P<0.01). Conclusion: miR-122-5p may induce MMP-9 expression by regulating TPA and thus inhibit the invasive and migratory ability of colorectal cancer SW480 cells, providing a new idea to overcome the metastasis study of colorectal cancer.
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2023, Vol. 29 
