氟伐他汀钠通过下调lncRNA PTPRG-AS1表达对结直肠癌SW620细胞增殖凋亡的影响

doi:10.3969/j.issn.1006-6233.2023.01.013

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Abstract Objective: To explore the effect and possible mechanism of fluvastatin sodium on the proliferation and apoptosis of colorectal cancer SW620 cells. Methods: SW620 cells and normal colorectal epithelial FHC cells were cultured in vitro, and the expression of lncRNA PTPRG-AS1 in the cells was detected by qRT-PCR. SW620 cells were divided into control group, fluvastatin sodium-low, medium and high groups, si-PTPRG-AS1 group, si-NC group, fluvastatin sodium+pcDNA-PTPRG-AS1 group, fluvastatin sodium+pcDNA group. CCK-8 method, clone formation assay and flow cytometry were used to detect cell viability, clone formation number, apoptosis rate, and Western blot was used to detect the expression of apoptosis-related proteins (cleaved-caspase3, cleaved-caspase9). Results: The expression of lncRNA PTPRG-AS1 in colorectal cancer SW620 cells was higher than that in FHC cells (4.38±0.31 vs 1.00±0.00, P<0.05). The cell viability and the number of colonies formed in the fluvastatin sodium-low, medium and high groups were lower than those in the control group(P<0.05), but the apoptosis rate and protein (cleaved-caspase3, cleaved-caspase9) expression were higher than those in the control group(P<0.05), and the expression of lncRNA PTPRG-AS1 was lower than that of the control group(P<0.05). The cell viability and the number of colonies formed in the si-PTPRG-AS1 group were lower than those in the si-NC group(P<0.05), but the apoptosis rate and protein (cleaved-caspase3, cleaved-caspase9) expression were higher than those in the si-NC group(P<0.05). The cell viability and the number of colonies formed in the fluvastatin sodium+pcDNA-PTPRG-AS1 group were higher than those in the fluvastatin sodium+pcDNA group(P<0.05), but the apoptosis rate and protein (cleaved-caspase3, cleaved-caspase9) expression were lower than those in the fluvastatin sodium+pcDNA group(P<0.05). Conclusion: Fluvastatin sodium may inhibit the proliferation and promote apoptosis of colorectal cancer SW620 cells by down-regulating the expression of lncRNA PTPRG-AS1.

Key words： Colorectal cancer Fluvastatin sodium LncRNA PTPRG-AS1 Cell proliferation Apoptosis

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.01.013 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I1/70

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