三七总皂苷抑制人类风湿关节炎滑膜成纤维细胞系增殖和促凋亡

doi:10.3969/j.issn.1006-6233.2023.01.010

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Abstract Objective: To investigate the effect and possible mechanism of Panax notoginseng saponins on the proliferation, apoptosis, migration and invasion of human rheumatoid arthritis synovial fibroblasts. Methods: MH7A cells were intervened with different doses (0.5, 1.0, 2.0 mg/mL) of Panax notoginseng saponins for 24h, and then cell proliferation, apoptosis, migration and invasion were detected by CCK-8, flow cytometry, scratch assay and Transwell, respectively. The expression of proteins (Ki-67, PCNA, E-cadherin and N-cadherin) were detected by Western blotting, and the expression of miR-335-5p was detected by qRT-PCR. The miR-335-5p mimic was transfected into MH7A cells, or the miR-335-5p inhibitor was transfected into MH7A cells and then intervened with 2.0mg/mL Panax notoginseng saponins for 24h, and then the same methods as above were used to detect cell proliferation, migration and invasion. Results: Compared with the control group, different doses of Panax notoginseng saponins decreased the A value, scratch healing rate, invasion number and the expression of Ki-67, PCNA and N-cadherin protein in MH7A cells (P<0.05), but increased the apoptosis rate and E-cadherin protein expression(P<0.05), and promoted the expression of miR-335-5p in cells (P<0.05). After up-regulating miR-335-5p, the A value, wound healing rate, invasion number and the expression of Ki-67, PCNA and N-cadherin protein in MH7A cells were all decreased (P<0.05), but the apoptosis rate and E-cadherin protein expression was decreased (P<0.05). Down-regulation of miR-335-5p reversed the effects of Panax notoginseng saponins on the proliferation, apoptosis, migration and invasion of MH7A cells. Conclusion: Panax notoginseng saponins may inhibit the proliferation, migration and invasion of rheumatoid arthritis synovial fibroblasts MH7A and promote cell apoptosis by up-regulating miR-335-5p.

Key words： Panax notoginseng saponins Rheumatoid arthritis Synovial fibroblasts Proliferation Apoptosis

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.01.010 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I1/53

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