LncRNA HCP5调节miR-27b-3p/H2AFZ轴对口腔鳞状细胞癌增殖侵袭迁移的机制研究

doi:10.3969/j.issn.1006-6233.2023.01.004

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Abstract Objective: To investigate the influences of long non-coding RNA (LncRNA) human leukocyte antigen complex P5 (HCP5) proliferation, invasion and migration of oral squamous cell carcinoma (OSCC) by regulating the microRNA (miR)-27b-3p/H2A histone family member Z (H2AFZ) axis. Methods: The cancer tissues and adjacent paracancerous tissues, OSCC cell lines (Tca-811, SCC-9, SCC-25, HN4) and human normal oral keratin-forming cells (hNOK) were collected from 48 OSCC patients treated surgically at our hospital from 2021 to 2022, and the LncRNA HCP5, miR-27b-3p, H2AFZ mRNA expression levels; logarithmic growth phase Tca-811 cells were divided into control group, small interfering RNA negative control (si NC) group, interfering HCP5 expression (si HCP5) group, si HCP5+ inhibitor negative control (inhibitor NC) group, si HCP5+ miR-27b-3p inhibitor (miR-27b-3p) group. Double luciferase was used to verify the targeting relationship between LncRNA HCP5 and miR-27b-3p, miR-27b-3p and H2AFZ; qRT-PCR to detect the expression levels of LncRNA HCP5, miR-27b-3p and H2AFZ mRNA; flow cytometry and MTT to detect apoptosis and proliferation changes of Tca-811 cells. Transwell assay was performed to detect changes in cell invasion and migration; Western blot to detect expression of cyclinD1, cleaved caspase-3, matrix metalloproteinase 2 and H2AFZ protein. Results: Compared with hNOK cells and adjacent tissues, the expressions of LncRNA HCP5 and H2AFZ mRNA in OSCC cells and tissues were increased, and the expression of miR-27b-3p was decreased (P<0.05). There was a targeting relationship between miR-27b-3p with LncRNA HCP5, H2AFZ. Compared with the si NC group and the control group, the apoptosis rate, cleaved caspase-3 and miR-27b-3p expressions in the si HCP5 group were higher than those in the si NC group and the control group (P<0.05), the OD570 (24 h, 48 h), the number of invasive and migrating cells and the expressions of CyclinD1, MMP-2, HCP5, H2AFZ were lower (P<0.05); inhibiting the expression of miR-27b-3p reversed the inhibitory effect of interfering with HCP5 on the malignant behavior of Tca-811 cells (P<0.05). Conclusion: Interfering with LncRNA HCP5 can inhibit the proliferation, invasion and migration of OSCC by targeting up-regulation of miR-27b-3p and inhibiting the expression of H2AFZ.

Key words： LncRNA HCP5 miR-27b-3p/H2AFZ axis Proliferation Invasion Migration Oral squamous cell carcinoma

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.01.004 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I1/18

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