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| Study on the Mechanism of miR-216a-3p and miR-128-3p Inhibiting the Phenotypic Deterioration of Oral Squamous Cell Carcinoma Cells by Regulating RGS17 |
| WANG Hui |
| Jinan Hospital of Traditional Chinese Medicine, Shandong Jinan 250012, China |
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Abstract Objective: To observe the effects of miR-216a-3p and miR-128-3p on the phenotypic expression of OSCC cells,and explore their potential mechanisms. Methods: The expression of miR-216a-3p,miR-128-3p and G protein signal regulator 17 (RGS17) in cancer tissues,adjacent tissues,human normal oral keratinocytes (hNOK) and OSCC cancer cells (SCC-9,SCC-25,HN4) were detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction (RT-qPCR); mimic NC group (transfected mimic),mimic-miR-216a-3p group (transfected miR-216a-3p mimic),mimic-miR-128-3p group (transfected miR-128-3p mimic),si-NC group (transfected si-NC),si-RGS17 group (transfected si-RGS17),mimic-miR-216a-3p+pcDNA group (co-transfected miR-216a-3p mimic and pcDNA),and mimic-miR-216a-3p+pcDNA-RGS17 group(co-transfected miR-216a-3p mimic and pcDNA-RGS17) were transfected into SCC-9 cells.5-bromo-2-deoxyuracil (EdU) staining and clonogenic assay were used to detect cell proliferation; Transwell test was used to detect the ability of cell migration and invasion; Annexin V / propidium iodide (PI) staining was used to detect the ability of apoptosis; Double luciferase report assay and RNA sedimentation assay were used to verify the targeting relationship; The RGS17 protein was detected by Western blot (WB). Results: Compared with adjacent tissues or hNOK cells,the expression of miR-216a-3p and miR-128-3p in OSCC tissues (stage Ⅰ,Ⅱ,Ⅲ) and cancer cells (SCC-9,SCC-25,HN4) was significantly decreased,and the expression of RGS17 was significantly increased (P<0.05).Compared with the mimic-NC group,the positive rate of EdU,the rate of clone formation,the number of migrating and invasive cells in the mimic-miR-216a-3p and mimic-miR-128-3p groups were significantly lower,and the apoptosis rate was significantly higher (P<0.05).The cells in si-RGS17 group had similar changes to those in mimic-miR-216a-3p and mimic-miR-128-3p cells.miR-216a-3p and miR-128-3p jointly targeted and negatively regulated RGS17.Overexpression of RGS17 significantly attenuated the proliferation,migration-invasion inhibition and apoptosis-promoting effects of miR-216a-3p and miR-128-3p on SCC-9 cells. Conclusion: MiR-216a-3p and miR-128-3p could inhibit the proliferation,migration and invasion of OSCC cells and promote apoptosis,which may be related to targeting RGS17.
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[1] Bugshan A,Farooq I.Oral squamous cell carcinoma:metastasis,potentially associated malignant disorders,etiology and recent advancements in diagnosis[J].F1000Res,2020,9(4):229.
[2] Ali Syeda Z,Langden SSS,Munkhzul C,et al.Regulatory mechanism of microRNA expression in cancer[J].Int Mol Sci,2020,21(5):1723.
[3] He B,Zhao Z,Cai Q,et al.miRNA-based biomarkers,therapies,and resistance in Cancer[J].Int Biol Sci,2020,16(14):2628-2647.
[4] Sun YH,An Q,Yan R,et al.MicroRNA-216a-5p suppresses esophageal squamous cell carcinoma progression by targeting KIAA0101[J].Oncol Rep,2020,44(5):1971-1984.
[5] Yu Q,Zhang N,Jiang Y,et al.RGS17 inhibits tumorigenesis and improves 5-fluorouracil sensitivity in nasopharyngeal carcinoma[J].Onco Targets Ther,2018,11(11):7591-7600.
[6] Qu XH,Shi YL,Ma Y,et al.LncRNA DANCR regulates the growth and metastasis of oral squamous cell carcinoma cells via altering miR-216a-5p expression[J].Hum Cell,2020,33(4):1281-1293.
[7] Zhou Y,Zhang S,Min Z,et al.Knockdown of circ_0011946 targets miR-216a-5p/BCL2L2 axis to regulate proliferation,migration,invasion and apoptosis of oral squamous cell carcinoma[J].BMC Cancer,2021,21(1):1085.
[8] Yao Y,Xu Q,Yan L,et al.MiRNA-128 and MiRNA-142 regulate tumorigenesis and EMT in oral squamous cell carcinoma through HOXA1[J].Cancer Manag Res,2020,12(10):9987-9997.
[9] Ma J,Wei H,Li X,et al.Hsa-miR-149-5p suppresses prostate carcinoma malignancy by suppressing RGS17[J].Cancer Manag Res,2021,13(3):2773-2783.
[10] Hu P,Zhou G,Zhang X,et al.Long non-coding RNA Linc00483 accelerated tumorigenesis of cervical cancer by regulating miR-508-3p/RGS17 axis[J].Life Sci,2019,34(10):116789.
[11] Wang SL,Dong XW,Zhao F,et al.MiR-203 inhibits cell proliferation,invasion,and migration of ovarian cancer through regulating RGS17[J].Biol Regul Homeost Agents,2021,35(3):1109-1115. |
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2022, Vol. 28 
