LYN通过AKT/mTOR信号通路对胃癌细胞增值及凋亡的影响

doi:10.3969/j.issn.1006-6233.2022.08.05

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Abstract Objective: To investigate the expression level of LYN in gastric cancer and the effect and mechanism of knockdown LYN expression on proliferation and apoptosis of gastric cancer cells.Methods: Immunohistochemistry was used to detect LYN expression in gastric cancer and adjacent tissues.Shrna-LYN was transfected into AGS (gastric adenocarcinoma) cells using Lipofectamine 2000, and untransfected gastric cancer cells were used as control. CCK8 and flow cytometry were used to detect the effects of LYN knockdown on AGS cell proliferation and apoptosis, respectively.Western blot analysis was used to investigate the changes in the expression levels of AKT/mTOR signaling pathway related proteins and apoptosis related proteins Bcl2, Bax, Caspase9 and Casapse3 induced by LYN knockdown, and to study its proliferation and apoptosis mechanism.Bioinformatics software was used to screen out the target gene miR-496, and western blot was used to detect the effect of transfection miR-496 on LYN expression. Rescue experiments were performed to analyze the interaction between miR-496 and LYN. Results: The sample number of LYN overexpression in gastric cancer tissues was 58 cases, which was significantly higher than that in paracancer tissues (28 cases) (P<0.05). After transfection 48 and 72h, the absorbance values of sh-LYN group were (0.0992±0.0346) and (1.0953±0.0379), which were significantly lower than those of NC group (1.4123±0.1161) and (1.6717±0.0120),(P<0.05).After transfection, the apoptosis rate of sh-LYN group (16.3567±0.5556) was significantly higher than that of NC group (P<0.05). After LYN knockdown, the expression levels of Bcl2 (0.4629 ± 0.0294), P-Akt (0.5565 ± 0.0473), P-MTOR (0.6167 ± 0.0459) and p70S6K (0.6049 ± 0.0570) decreased compared with NC control group. The expression of Bax (1.407 ± 0.0528), Caspase-9 (1.5200 ± 0.1063), caspase-3 (1.4170 ± 0.0751) increased. Transfection of miR-496 could inhibit LYN expression in AGS cells. Conclusion: Knockdown LYN expression can inhibit AKT/mTOR signaling pathway, thereby inhibiting proliferation and promoting apoptosis of gastric cancer cells, and its effect on gastric cancer cells is regulated by miR-496.

Key words： LYN Gastric cancer Cell proliferation Cell apoptosis AKT/mTOR signaling pathway

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	SU Rui
	LI Yingjian
	LIU Peng
	et al

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SU Rui,LI Yingjian,LIU Peng, et al. Effects of LYN on Proliferation and Apoptosis of Gastric Cancer Cells through AKT/mTOR Signaling Pathway[J]. HeBei Med, 2022, 28(8): 1256-1261.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2022.08.05 OR http://www.hbyxzzs.cn/EN/Y2022/V28/I8/1256

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