Nckα基因沉默对上皮性卵巢癌细胞恶性生物学行为的影响

doi:10.3969/j.issn.1006-6233.2022.07.03

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Abstract Objective: To investigate the effect of Nckα gene silencing on the malignant biological behavior of epithelial ovarian cancer cells. Methods: The expression characteristics of Nckα were analyzed in normal ovarian tissue sections,benign ovarian tissue sections and ovarian cancer tissue sections.The effects of Nckα silencing on ovarian cancer cell lines SKOV3 and OVCAR-3 were detected by immunofluorescence,cell invasion,migration,cell proliferation,subcutaneous xenograft animal model,and Western blot. Results: Nckα gene silencing could reduce the invasion and migration ability of ovarian cancer cells.The absorbance of SKOV3 and OVCAR3 cells with Nckα silence was significantly lower than that of the control group,and the absorbance of SKOV3 and OVCAR3 cells with Nckα overexpression was significantly higher than that of the control group (P<0.05).The tumor volume of Nckα silenced group was lower than that of control group (P<0.05).The protein expressions of PI3K P110 α,p70S6K,AKT and P-Akt in SKOV3 cells and OVCAR3 cells silenced by Nckα were significantly lower than those in the control group.The protein expressions of PI3K P110 α,p70S6K,AKT and P-Akt in SKOV3 cells and OVCAR3 cells with Nckα overexpression were significantly higher than those in the control group (P<0.05). Conclusion: Nckα gene silencing can reduce the proliferation and migration ability of epithelial ovarian cancer cells,and delay the growth rate of subcutaneous graft tumor,which may be related to the regulation of PI3K P110 α,p70S6K,AKT and P-Akt protein expression by Nckα gene silencing.

Key words： Nckα gene Ovarian cancer Epithelial lesions

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2022.07.03 OR http://www.hbyxzzs.cn/EN/Y2022/V28/I7/1066

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