circ_0011385和miR-330-3p在结肠癌中的表达及其对结肠癌细胞恶性行为的影响

doi:10.3969/j.issn.1006-6233.2022.06.07

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Abstract Objective: To investigate the expression of circ_0011385 and miR-330-3p in colon cancer tissues, and further study the effect of circ_0011385 targeting miR-330-3p on the malignant behavior of colon cancer cells (SW1116). Methods: RT-qPCR was performed to detect circ_0011385 and miR-330-3p expression in colon cancer tissues, and further analyze the relationship between the expression levels of circ_0011385 and miR-330-3p and the clinico- pathological characteristics of colon cancer patients. The relationship between circ_0011385 and miR-330-3p was confirmed by dual luciferase reporter assays. SW1116 cells were transfected with si-circ_0011385, si-NC, si-circ_0011385+anti-miR-NC, or si-circ_0011385+anti-miR-330-3p, respectively. Transwell and flow cytometry analysis were used to analyze cell migration, invasion and apoptosis. Results: In colon cancer tissues, circ_0011385 was highly expressed, while miR-330-3p was lowly expressed, and the expression levels of the two were negatively correlated (r=-0.8924). Circ_0011385 and miR-330-3p expression was not correlated with the age and tumor size of colon cancer patients (P>0.05), but it was significantly correlated with the degree of differentiation, TNM stage, and lymph node metastasis (P<0.05). MiR-330-3p is a target gene of circ_0011385. After silencing the expression of circ_0011385, the apoptosis rate of SW1116 cells and the expression level of miR-330-3p were significantly increased (P<0.05), and the number of cell migration and invasion was significantly reduced (P<0.05). Inhibition of miR-330-3p expression significantly reduced the effect of silencing circ_0011385 on SW1116 cell apoptosis, migration and invasion (P<0.05). Conclusion: Circ_0011385 is highly expressed, while miR-330-3p is lowly expressed in colon cancer. Silencing circ_0011385 induces cell apoptosis and inhibit the migration and invasion in colon cancer by up-regulating miR-330-3p expression.

Key words： Colon cancer Circ_0011385 MiR-330-3p Apoptosis Migration Invasion

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	DONG Jinhua
	WU Baohua
	YAN dancuo

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DONG Jinhua,WU Baohua,YAN dancuo. Expression of Circ_0011385 and MiR-330-3p in Colon Cancer and the Influence on the Malignant Behavior of Colon Cancer Cells[J]. HeBei Med, 2022, 28(6): 909-913.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2022.06.07 OR http://www.hbyxzzs.cn/EN/Y2022/V28/I6/909

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